INSTRUCTION MANUAL

HI98193
Dissolved Oxygen
BOD/OUR/SOUR Meter

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 Dear Customer, Thank you for choosing a Hanna Instruments product.
Please read this instruction manual carefully before using the instrument.
This manual will provide you with the necessary information for correct use
of the instrument, as well as a precise idea of its versatility.
If you need additional technical information, do not hesitate to e‑mail
us at [email protected] or view our worldwide contact list at
www.hannainst.com.

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All rights are reserved. Reproduction in whole or in part is prohibited without the written consent of
the copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895, USA.
 PRELIMINARY EXAMINATION .............................................................................................. 4

TABLE OF CONTENTS
GENERAL DESCRIPTION ...................................................................................................... 5
FUNCTIONAL DESCRIPTION ................................................................................................. 6
SPECIFICATIONS ................................................................................................................ 8
PROBE FUNCTIONAL DESCRIPTION ...................................................................................... 9
PROBE CONNECTION AND PREPARATION ............................................................................. 10
OPERATIONAL GUIDE ......................................................................................................... 11
OUR MEASUREMENT .......................................................................................................... 20

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SOUR MEASUREMENT ........................................................................................................ 22
TEMPERATURE MEASUREMENT ........................................................................................... 25

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DO CALIBRATION PROCEDURE ............................................................................................. 25
GOOD LABORATORY PRACTICE (GLP) .................................................................................... 29

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SETUP ............................................................................................................................... 30

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LOGGING ........................................................................................................................... 42
AutoEnd ............................................................................................................................ 46
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PRESSURE CALIBRATION .................................................................................................... 47
TEMPERATURE CALIBRATION (for technical personnel only) .................................................... 49
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PC INTERFACE .................................................................................................................... 52
BATTERIES REPLACEMENT ................................................................................................... 59
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PROBE MAINTENANCE ........................................................................................................ 60

TROUBLESHOOTING GUIDE ................................................................................................. 61
ACCESSORIES .................................................................................................................... 62
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 Remove the instrument from the packing material and examine it carefully to make sure that no
PRELIMINARY EXAMINATION

damage has occurred during shipping. If there is any damage, notify your Dealer or the nearest
Hanna Customer Service Center.
Each instrument is supplied with:
• HI764073 Polarographic probe with built-in temperature sensor and 4 m (13.4’) cable
• HI76407A Membrane cap (2 pcs.)
• HI7040 Bicomponent Zero Oxygen Solution

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• HI7041S Electrolyte solution (30 mL)

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• HI920015 Micro USB cable

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• HI92000 PC Software
• DO protective cap

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• 100 mL Plastic Beaker (2 pcs.)
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• 1.5V AA Batteries (4 pcs.)
• Instruction manual and Quick Reference Guide
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• Certificate

Note: Save all packing material until you are sure that the instrument functions correctly. All
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defective items must be returned in the original packing with the supplied accessories.
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 The HI98193 is a state of art, microprocessor based, dissolved oxygen instrument with many automated

GENERAL DESCRIPTION
and specific application features, designed to provide laboratory results and accuracy under harsh
industrial conditions.
All measurements are automatically compensated for temperature. Manual salinity compensation in
water allows direct determination of dissolved oxygen in saline waters. With its internal barometer,
the instrument is able to automatically compensate for changes in barometric pressure so there is no
need for charts, altitude information or external barometric pressure information.
The instrument contains built-in application software for the calculation of Biochemical Oxygen

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Demand (BOD), Oxygen Uptake Rate (OUR) and Specific Oxygen Uptake Rate (SOUR).

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HI98193’s calibration has been greatly simplified when compared to other dissolved oxygen
instruments. It is provided with a series of new features which add entirely new dimensions to the

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measurement of DO, by allowing the user to dramatically improve the reliability of the measurement:
• One or two points automatic dissolved oxygen calibration.
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• One point manual dissolved oxygen calibration using a value in milligrams per liter or percent
saturation entered by the user.
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• One or two points user temperature calibration.
• Messages on the graphic LCD for an easy and accurate calibration.
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• User-selectable “calibration time out” to remind when a new calibration is necessary.

Moreover, the meter offers an extended temperature range from -20.0 °C to 120.0 °C
(-4.0 °F to 248.0 °F).
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Other features include:

• Log on demand up to 400 samples.
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• Auto Hold feature, to freeze first stable reading on the LCD.

• GLP feature, to view last calibration data.
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• PC interface.
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 FRONT VIEW
FUNCTIONAL DESCRIPTION

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1) Liquid Crystal Display (LCD).

2) F1, F2, F3 functional keys.
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3) / keys to manually increase/decrease the parameters or to scroll through the menu.

4) ON/OFF ( ) key, to turn the instrument ON and OFF.
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5) LIGHT ( ) key, to toggle display backlighting.

6) GLP key, to display Good Laboratory Practice information.
7) CAL key, to enter/exit calibration mode.
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8) SETUP key, to enter/exit SETUP mode.

9) RCL key, to enter/exit view logged data mode (RCL means RECALL).
10) MODE key to change DO measuring unit when in DO measurement mode, or to toggle between
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standard and pressure in DO calibration.

11) RANGE key, to switch between DO, BOD, OUR AND SOUR.
12) HELP key to enter/exit contextual help.
13) ESC key to leave current mode, exit calibration, setup, help, etc.

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 TOP VIEW

FUNCTIONAL DESCRIPTION
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14) Electrode DIN connector.

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15) USB connector.
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 SPECIFICATIONS
Dissolved Oxygen Range 0.00 to 50.00 mg/L / 0.0 to 600.0 % saturation
Resolution 0.01 mg/L / 0.1 % saturation
Accuracy ±1.5 % of reading ±1 digit
Barometric Pressure Range 450 to 850 mmHg
Resolution 1 mmHg
Accuracy ±3 mmHg within ±15 % from the calibration point

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Temperature Range -20.0 to 120.0 °C (-4.0 to 248.0 °F)

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Resolution 0.1 °C (0.1 °F)

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Accuracy ±0.2 °C (±0.4 °F) (excluding probe error)

DO Calibration • One or two points automatic calibration at 100 % (8.26 mg/L)

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and 0 % (0 mg/L).
• One point manual using a value entered by the user in %
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saturation or mg/L.
Temperature Calibration One or two point at any in range temperature value
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Pressure Calibration One point at any in range pressure value
Temperature Automatic from 0.0 to 50.0 °C (32.0 to 122.0 °F)
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Compensation
Pressure Compensation Automatic from 450 to 850 mmHg
Salinity Compensation Automatic from 0 to 70 g/L
DO Probe HI764073 Polarographic Probe
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LOG On demand, 400 samples

1.5V AA batteries (4 pcs.) / approx. 200 hours of continuous use
Battery Type / Life
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without backlight (50 hours with backlight)

Auto Power Off User selectable: 5, 10, 30, 60 minutes or disabled
PC Connectivity Opto-isolated USB
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Dimensions 185 x 93 x 35.2 mm (7.3 x 3.6 x 1.4”)

Weight 400 g (14.2 oz)
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Environment 0 to 50 °C (32 to 122 °F) max. RH 100% IP67

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 PROBE FUNCTIONAL DESCRIPTION
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1. DO Probe
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2. Protective Cap
3. Watertight Shielded Cable
4. Polypropylene Probe Body
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5. Temperature Sensor
6. O-Ring Seal
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7. Silver Chloride Anode

8. Platinum Cathode (sensor)
9. Oxygen Permeable PTFE® Membrane
10. Membrane Cap

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 To take measurements, connect the DO probe to the meter securely by
PROBE CONNECTION AND PREPARATION

aligning the pins with the socket located on the top of the meter, pushing
the plug in and tightening the threaded ring.
Probes shipped from Hanna Instruments are dry. To hydrate the probe
and prepare it for use, connect it to the meter and proceed as follows:
1. Remove the red and black plastic cap. This cap is for shipping
purposes and can be thrown away.

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2. Wet the sensor by soaking the bottom 21/2 cm (1’’) of the probe in
electrolyte (HI7041S) for 5 minutes.

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3. Rinse the membrane cap (HI76407A) supplied in the kit with the
meter with electrolyte solution while shaking it gently. Refill with

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4. Tap gently the sides of the membrane cap with your finger tip to
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ensure that no air bubbles are trapped. To avoid damaging the
membrane, do not tap it directly on the bottom.
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5. Make sure that the rubber O-Ring sits properly inside the membrane
cap.
6. With the sensor facing down, slowly screw the cap clockwise. Some
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electrolyte will overflow.

When not in use and during polarization (see PROBE CONDITIONING
page 11), use the protective transparent cap supplied in the kit with
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the meter.
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 OPERATIONAL GUIDE
INITIAL PREPARATION
The instrument is supplied complete with batteries. See Batteries Replacement for details, page 59.
To prepare the instrument for field measurements close the USB communication socket and all unused
connector sockets with proper stopper (to ensure waterproof protection).
Connect the DO probe to the 7-pin connector. Make sure that the probe sleeve is properly inserted
and tighten the threaded ring.
Turn the instrument ON by pressing ON/OFF key.

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At start-up the display will show the Hanna logo for a few seconds, followed by the percentage indication

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of the remaining battery life, then enters the probe conditioning mode. The probe will be conditioned

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for one minute, and afterwards the instrument will enter measurement mode.
If the DO probe is not connected or is damaged, the conditioning period is skipped.
To save battery life, the Auto Power Off feature turns the instrument off after a set period (default 30 min)

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if no button is pressed. To set another period or to disable this feature, see SETUP menu on page 30.
The Auto Light Off backlight feature turns the backlight off after a set period (default 1 min) with no
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buttons pressed. To set another period or to disable this feature, see SETUP on page 30.
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PROBE CONDITIONING
Probe polarization is essential for stable, accurate measurements. Probe polarization ensures the
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electrodes are conditioned and any oxygen dissolved in the electrolyte is consumed, so the only oxygen
present will be oxygen diffusing from sample across the PTFE membrane. It is recommended to wait
for at least 15 minutes to ensure precise conditioning of the probe.
When the probe is properly polarized, dissolved oxygen is consumed as it passes through the PTFE
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membrane into the electrolyte at the platinum cathode, and an accurate DO reading is displayed.
When the probe is not polarized, oxygen from the test solution as well as oxygen present in the
electrolyte solution are detected, resulting in an incorrect DO reading.
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 SALINITY COMPENSATION
OPERATIONAL GUIDE

If the sample contains significant concentration of salinity, the read out values must be corrected, taking
into account the lower degree of oxygen solubility in this situation. Before taking any DO measurements
remember to set the salinity value from the SETUP menu (page 30).
The salinity affects the DO concentration, decreasing its value. The table below shows the maximum
oxygen solubility at various temperatures and salinity levels.
°C Salinity (g/l) at Sea Level °F

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0 g/l 10 g/l 20 g/l 30 g/l 35 g/l

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0 14.60 13.64 12.74 11.90 11.50 32.0

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2 13.81 12.91 12.07 11.29 10.91 36.5
4 13.09 12.25 11.47 10.73 10.38 39.2
6 12.44 11.65 10.91 10.22 9.89 42.8

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8 11.83 11.09 10.40 9.75 9.44 46.4
10 11.28 10.58 9.93 9.32 9.03 50.0
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12 10.77 10.11 9.50 8.92 8.65 53.6
14 10.29 9.68 9.10 8.55 8.30 57.2
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16 9.86 9.28 8.73 8.21 7.97 60.8
18 9.45 8.90 8.39 7.90 7.66 64.4
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20 9.08 8.56 8.07 7.60 7.38 68.0

22 8.73 8.23 7.77 7.33 7.12 71.6
24 8.40 7.93 7.49 7.07 6.87 75.2
25 8.24 7.79 7.36 6.95 6.75 77.0
26 8.09 7.65 7.23 6.83 6.64 78.8
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28 7.81 7.38 6.98 6.61 6.42 82.4

30 7.54 7.14 6.75 6.39 6.22 86.0
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32 7.29 6.90 6.54 6.19 6.03 89.6

34 7.05 6.68 6.33 6.01 5.85 93.2
36 6.82 6.47 6.14 5.83 5.68 96.8
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38 6.61 6.28 5.96 5.66 5.51 100.4

40 6.41 6.09 5.79 5.50 5.36 104.0
42 6.22 5.93 5.63 5.35 5.22 107.6
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44 6.04 5.77 5.48 5.21 5.09 111.2

46 5.87 5.61 5.33 5.07 4.97 114.8
48 5.70 5.47 5.20 4.95 4.85 118.4
50 5.54 5.33 5.07 4.83 4.75 122.0

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 Note: The relationship between salinity and chlorinity for seawater is given by the equation

OPERATIONAL GUIDE
below: Salinity (g/l) = 1.80655 Chlorinity (g/l)
BAROMETRIC PRESSURE COMPENSATION
The dissolved oxygen saturation value varies with pressure, so it is important to compensate the effect
that pressure has on DO measurements.
Altitude, Meters above Sea Level
°C 0 300 600 900 1200 1500 1800 2100 2400 2700 3000 3300 3600 3900 4000 °F

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m m m m m m m m m m m m m m m

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0 14.6 14.1 13.6 13.1 12.6 12.1 11.7 11.2 10.8 10.4 10.0 9.7 9.3 9.0 8.9 32.0

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2 13.8 13.3 12.8 12.4 11.9 11.5 11.0 10.6 10.2 9.9 9.5 9.2 8.8 8.5 8.4 35.6
4 13.1 12.6 12.2 11.7 11.3 10.9 10.5 10.1 9.7 9.3 9.0 8.7 8.4 8.0 7.9 39.2

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6 12.4 12.0 11.5 11.1 10.7 10.3 9.9 9.6 9.2 8.9 8.6 8.2 7.9 7.6 7.5 42.8

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8 11.8 11.4 11.0 10.6 10.2 9.8 9.5 9.1 8.8 8.4 8.1 7.8 7.5 7.3 7.2 46.4
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10 11.3 10.9 10.5 10.1 9.7 9.4 9.0 8.7 8.4 8.1 7.8 7.5 7.2 6.9 6.8 50.0
12 10.8 10.4 10.0 9.6 9.3 8.9 8.6 8.3 8.0 7.7 7.4 7.1 6.9 6.6 6.5 53.6
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14 10.3 9.9 9.6 9.2 8.9 8.5 8.2 7.9 7.6 7.4 7.1 6.8 6.6 6.3 6.2 57.2
16 9.9 9.5 9.2 8.8 8.5 8.2 7.9 7.6 7.3 7.0 6.8 6.5 6.3 6.1 6.0 60.8
18 9.5 9.1 8.8 8.5 8.1 7.8 7.6 7.3 7.0 6.8 6.5 6.3 6.0 5.8 5.7 64.4
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20 9.1 8.8 8.4 8.1 7.8 7.5 7.3 7.0 6.7 6.5 6.2 6.0 5.8 5.6 5.5 68.0
22 8.7 8.4 8.1 7.8 7.5 7.2 7.0 6.7 6.5 6.2 6.0 5.8 5.6 5.4 5.3 71.6
24 8.4 8.1 7.8 7.5 7.2 7.0 6.7 6.5 6.2 6.0 5.8 5.6 5.4 5.2 5.1 75.2
25 8.3 8.0 7.7 7.4 7.1 6.8 6.6 6.4 6.1 5.9 5.7 5.5 5.3 5.1 5.0 77.0
26 8.1 7.8 7.5 7.2 7.0 6.7 6.5 6.2 6.0 5.8 5.6 5.4 5.2 5.0 4.9 78.8
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28 7.8 7.5 7.3 7.0 6.7 6.5 6.2 6.0 5.8 5.6 5.4 5.2 5.0 4.8 4.7 82.4
30 7.6 7.3 7.0 6.8 6.5 6.3 6.0 5.8 5.6 5.4 5.2 5.0 4.8 4.6 4.6 86.0
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32 7.3 7.0 6.8 6.5 6.3 6.1 5.8 5.6 5.4 5.2 5.0 4.8 4.7 4.5 4.4 89.6
34 7.1 6.8 6.6 6.3 6.1 5.9 5.6 5.4 5.2 5.0 4.9 4.7 4.5 4.3 4.3 93.2
36 6.8 6.6 6.3 6.1 5.9 5.7 5.5 5.3 5.1 4.9 4.7 4.5 4.4 4.2 4.1 96.8
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38 6.6 6.4 6.1 5.9 5.7 5.5 5.3 5.1 4.9 4.7 4.5 4.4 4.2 4.1 4.0 100.4
40 6.4 6.2 5.9 5.7 5.5 5.3 5.1 4.9 4.7 4.6 4.4 4.2 4.1 3.9 3.9 104.4
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42 6.2 6.0 5.8 5.6 5.3 5.2 5.0 4.8 4.6 4.4 4.3 4.1 4.0 3.8 3.8 107.6
44 6.0 5.8 5.6 5.4 5.2 5.0 4.8 4.6 4.5 4.3 4.1 4.0 3.8 3.7 3.7 111.2
46 5.8 5.6 5.4 5.2 5.0 4.8 4.7 4.5 4.3 4.2 4.0 3.9 3.7 3.6 3.5 114.8
48 5.7 5.5 5.3 5.1 4.9 4.7 4.5 4.4 4.2 4.0 3.9 3.7 3.6 3.5 3.4 118.4
50 5.5 5.3 5.1 4.9 4.7 4.6 4.4 4.2 4.1 3.9 3.8 3.6 3.5 3.4 3.3 122.0

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 The HI98193 meter contains a built-in barometer, and it is able to automatically compensate for
OPERATIONAL GUIDE

changes in barometric pressure. If another pressure value than the barometer’s reading is to be used,
then the manual pressure feature must be enabled from the SETUP menu (see page 30), and afterwards
the pressure value can be set using the / keys.
The table below contains a conversion altitude (m) to pressure (mmHg) for the altitude values from
the previous table.
Pressure (mmHg) Altitude (m)

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Altitude

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0 300 600 900 1200 1500 1800 2100 2400 2700 3000 3300 3600 3900 4000

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Pressure

760 732 705 679 654 630 607 584 563 542 522 503 484 467 461

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DO MEASUREMENTS
Make sure the probe’s protective cap has been removed.
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In order to take accurate dissolved oxygen measurements make sure that the instrument has been
calibrated (see page 25 for details).
Press RANGE to access the DO measure screen. If necessary press MODE to change the measuring unit.
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Immerse the tip of the probe into the sample to be tested. Allow approximately one minute for the
reading to stabilize (hourglass symbol turns off).
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On the screen are displayed:

• Dissolved Oxygen reading in the selected unit (% saturation or mg/L)
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• Temperature reading in the selected unit (°C or °F)

• Pressure reading in the selected unit (mmHg, inHg, atm, psi, kPa, mbar). If the Manual Pressure
option is enabled ( displayed in front of the pressure value) the pressure value can be changed
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using the / keys.

For accurate dissolved oxygen measurements, a water movement of 0.3 m/s is required. This is to
ensure that the oxygen-depleted membrane surface is constantly replenished. A moving stream will
provide adequate circulation.

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 BOD MEASUREMENTS

OPERATIONAL GUIDE
Biochemical oxygen demand (BOD) is an indicator for the concentration of biodegradable organic
matter present in a sample of water. It can be used to infer the general quality of the water and its
degree of pollution. BOD measures the rate of oxygen uptake by microorganisms in a sample of water
at a fixed temperature and over a given period of time. To ensure that all other conditions are equal, a
very small amount of microorganism seed is added to each sample being tested. This seed is typically
generated by diluting activated sludge with deionized water. The samples are kept at 20 °C in the
dark and tested for dissolved oxygen (DO) after five days. The loss of dissolved oxygen in the sample,

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once corrections have been made for the degree of dilution, is called the BOD5.

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Before measuring BOD, remember to set the BOD configuration from the SETUP menu (see page 30).

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Press RANGE to access the BOD measurement screen.

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Press BOD Data to view the BOD initial data management screen.
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Press Compute to evaluate the BOD for a specified sample (available only when the measurement
is stable and at least one initial BOD data record has been memorized).
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BOD initial data management screen

BOD Data is pressed while in BOD measurement screen.
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Press Add new to add a new BOD initial data record (the key is available only when the measurement
is stable). A 200 records memory space is available for BOD initial data.
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Press View ID to view the saved BOD initial values (the key is available only when at least one initial
BOD data record has been memorized).
By pressing Add New the BOD Parameters screen will be displayed:
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 BOD Parameters:
OPERATIONAL GUIDE

• Bottle ID: a number used to identify a specific bottle.

Range: 0000 to 9999.
• The type of the Sample: Sample or Seed.
• Bottle Volume: the total volume of the BOD bottle.
Range: 0.1 to 300.0 mL.
• Sample Volume: the volume of sample in the BOD bottle.

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Range: 0.1 to 300.0 mL (for a seed sample this value is 0.0 mL and cannot be set).

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• Seed Volume: the volume of seed in the BOD bottle.
Range: 0.0 to 300.0 mL.

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Press / keys to modify the selected parameter’s value.
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Press Save to save the BOD parameters and the initial DO, temperature, pressure and salinity values
for the specified bottle.
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If a bottle with the same ID already exists, the instrument will ask for replacement confirmation.
Press Replace to replace the existing record, or ESC to return to the previous screen without replacing.
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When a new record is saved the meter will display a message indicating the remaining free BOD
initial data space in %.
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By pressing View ID a list of all the saved BOD initial data records will be displayed. The seed records
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will have the symbol “*” displayed after the bottle ID.

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 Use the / keys to scroll the list of BOD initial data records.

OPERATIONAL GUIDE
Press More to view detailed information for the selected record.

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Press Pg Up/Pg Down to view the next/previous screen of information.

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Use the / keys to view the detailed information about the next/previous record.
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If Delete is pressed, use the / keys to highlight the record to be deleted and then press CFM.
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Press ESC to exit.

If Delete All is pressed the instrument asks for confirmation. Press CFM to confirm or ESC to exit
without deleting.
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BOD evaluation
From the BOD measure screen press Compute to evaluate the BOD for a specified sample. The
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following screen will be displayed.

If the date of the current measurement is previous to the date of the selected measurement then the
Eval. BOD key will not be displayed.
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Press MORE to view detailed information for the selected record.

Use / keys to select the bottle for BOD evaluation.
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 Press Eval. BOD to compute the BOD for the selected bottle. If the time difference between the current
OPERATIONAL GUIDE

reading and the selected reading is less than 1 day the instrument will ask for record replacement
confirmation, and the BOD can’t be evaluated.

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Press CFM to replace the selected records DO, temperature, pressure and salinity values with the

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current values.

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Press ESC to return to the previous screen without replacing.

n If the conditions regarding the time difference are met, after pressing the Eval. BOD key, the instrument

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will display the computed BOD value.
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Press LOG to save the BOD result.

Press ESC to return to the BOD measure screen.
If the final DO reading or DO loss doesn’t meet the criteria for BOD measurements set from the
instrument’s setup, a warning message will be displayed.
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Press any key to clear the warning message from the display or press HELP to view detailed infor‑
mation about the warning.
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 Note: If the Autodelete BOD start data option is enabled in SETUP (see page 30), when the

OPERATIONAL GUIDE
BOD result is saved (LOG key is pressed) the corresponding BOD initial data record will be
automatically deleted from the instrument’s memory.

Seed Correction
In case that the BOD was evaluated for a seeded sample and the list of the saved seed BOD values
is not empty, the Correct functional key will be displayed.
Press Correct to view the list of the saved seed BOD values.

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Select the desired seed BOD and then press Correct to compute the corrected BOD value. The
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instrument will display the corrected BOD value.
If the information about the BOD of a certain seed doesn’t exist at the moment of the BOD
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evaluation for a seeded sample, the sample’s BOD can be corrected at a later time from the BOD
recall menu (view logged BOD data).
In order to perform a seed correction from the BOD recall, press RCL key from the BOD
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measurement screen to enter BOD recall, select the desired BOD record and press More.
The instrument will display a complete set of information about the selected record.
Press Correct to view the list of the seed values.
Select the desired seed BOD and then press Correct to compute the corrected BOD value. The new
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BOD value will be displayed.

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Note: If the final DO value is greater than the initial DO value an error message will be
displayed.
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 The OUR is used to determine the oxygen consumption or respiration rate in water. It is defined as
OUR MEASUREMENT

the mg/L of oxygen consumed per hour.

The following equation is used for OUR determination:
where:

DOSTART = Dissolved oxygen level at start of test

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DOEND = Dissolved oxygen level at end of test

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tELAPSED = Elapsed time of test in seconds

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total volume/sample volume = Dilution factor of sample
Before starting an OUR test remember to set the OUR configuration from the SETUP menu (page 30).

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OUR measurement screen:
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Press Start to begin a new OUR test.

If the DO value is less than the minimum start DO value the meter will display an error message, and
the test cannot be started.
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w w

If the minimum start DO condition is met the instrument will display the instantaneous oxygen
consumption rate and the amount of time that has passed from the beginning of the test.
w
w

If the DO reading is less than the minimum end DO value set during OUR configuration a warning icon
will be displayed and a beep will be heard every two seconds. Press Stop to stop the test and the beeper.

20
 OUR MEASUREMENT
To end the OUR test before the maximum time interval set during OUR configuration press Stop.
If Stop is pressed before the minimum time for the OUR test has elapsed, the instrument will display
a warning message.

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t
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Press Resume to continue the test or Stop to end the test.

n
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At the end of the test the meter will display the computed OUR value and the duration of the test.
Press LOG to save a complete set of data regarding the OUR test.
eo .i
Press Start to begin a new OUR test.
.n ls
w oo

Notes: If at the end of the test the DO reading is less than the minimum end DO value set
during OUR configuration, a warning message will be displayed.
w .t
w w

Press any key to clear the message from the screen, or press HELP to view detailed information
w

about the warning.

If the DO value at the end of the test is greater than the DO value from the beginning of the
test an error message will be displayed.
w

Press Start to begin a new OUR test or ESC to return to the OUR measure screen.

21
 The Specific Oxygen Uptake Rate (SOUR), also known as the oxygen consumption or respiration rate,
SOUR MEASUREMENT

is defined as the milligram of oxygen consumed per gram of volatile suspended solids (VSS) per
hour. This quick test has many advantages: rapid measure of influent organic load and biodegradability,
indication of the presence of toxic or inhibitory wastes, degree of stability and condition of a sample,
and calculation of oxygen demand rates at various points in the aeration basin.
The following equation is used for SOUR determination:

SOUR = OUR / Solids Weight

cs h
t
where:
OUR is the Oxygen Uptake Rate (see equation on page 20)

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ni .t
Solids Weight is the Total solids or the Volatile suspended solids weight in g/L
Temperature correction:

n The SOUR value is corrected to 20 °C (68 °F) according to the Farrel and Bhide equation:

.c
SOUR20=SOURTxΘ(20-T)
eo .i
Where T is the measured temperature in °C and Θ is a temperature dependent variable:
Θ= 1.05 for T above 20 °C
.n ls
Θ= 1.07 for T below 20 °C
This calculation is valid only for temperature values in the range 10 to 30 °C. Temperature correction is
performed only if the option SOUR @ 20 °C is enabled from SOUR configuration in the setup menu.
w oo

Before starting a SOUR test remember to set the SOUR configuration from the setup menu.
SOUR measurement screen:
w .t
w w

If the SOUR value is corrected to 20 °C (68 °F) the message “@20°C”, or ”@68°F” according to
the currently selected temperature unit, will be displayed above the measured temperature.
w

Press Start to begin a new SOUR test.

If the DO value is less than the minimum start DO value, the meter will display an error message,
and the test cannot be started.
w

22
 If the minimum start DO condition is met the instrument will display the instantaneous specific oxygen

SOUR MEASUREMENT
consumption rate and the amount of time that has passed from the beginning of the test.

In case that the SOUR value is corrected to 20 °C (68 °F) and the measured temperature isn’t in the

cs h
t
range 10 to 30 °C the temperature value will blink to alert that the temperature correction isn’t valid.

o.
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.c
eo .i
If the DO reading is less than the minimum end DO value set during SOUR configuration, a warning
icon will be displayed and a beep will be heard every two seconds. Press Stop to stop the test and
.n ls
the beeper.
w oo

To end the SOUR test before the maximum interval set during SOUR configuration press Stop.
If Stop is pressed before the minimum time for the SOUR test has elapsed the instrument will display
w .t

a warning message.
w w w
w

23
 Press LOG to save a complete set of data regarding the SOUR test.
SOUR MEASUREMENT

Press Start to begin a new SOUR test.

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t
Notes: If the DO reading is less than the minimum end DO value set during SOUR configuration,

o.
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a warning message will be displayed.
Press any key to clear the message from the screen, or press HELP to view detailed information

n about the warning.

.c
If the DO value is greater than the DO value from the beginning of the test an error message
will be displayed.
eo .i
.n ls
w oo

Press Start to begin a new SOUR test or ESC to return to the SOUR measure screen.
w .t
w w w
w

24
 The DO probe has a built-in temperature sensor.

TEMPERATURE MEASUREMENT
The measured temperature is indicated on the display.
Allow the probe to reach thermal equilibrium before taking any measurements. This can take several
minutes. The greater the difference between the temperature at which the probe was stored and the
temperature of the sample, the longer the time will be.

Note: If “----” is displayed instead of the measured temperature, the DO probe is not properly
connected or the temperature is out of range. This also indicates the possibility of a broken

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probe cable.

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.c
eo .i
The following options are available for the Dissolved Oxygen calibration:

DO CALIBRATION PROCEDURE
• One point automatic zero calibration at 0% saturation or 0 mg/L
.n ls
• One point automatic slope calibration at 100% saturation or 8.26 mg/L
• Two points automatic calibration at 0% saturation (0 mg/L) and 100% saturation (8.26 mg/L)
• One point manual calibration using a standard value set by the user in % saturation or mg/L
w oo

When automatic calibrations are performed it is assumed that the standard value is the saturated DO
value at 25 °C, 0 g/L salinity and 760 mmHg.
When manual calibrations are performed it is assumed that the standard value is the DO value at the
current pressure, temperature and salinity.
w .t

INITIAL PREPARATION
Make sure the probe is ready for measurements (see probe connection and preparation on page 10),
w w

i.e. the membrane is filled with electrolyte and the probe is connected to the meter.
For an accurate calibration, it is recommended to wait for at least 15 minutes to ensure precise
conditioning of the probe.
w

Remove the protective cap from the DO probe.

Make sure the salinity value has been set to the salinity of the standard (see SETUP for details).
One point automatic zero calibration
w

Submerge the probe into HI7040 zero oxygen solution and stir gently
for 2-3 minutes.
Press CAL. The calibration menu will be displayed.
Press DO to select the DO calibration.

25
 The DO calibration screen will be displayed and the standard 0% saturation (or 0 mg/L, depending
DO CALIBRATION PROCEDURE

on the currently selected measuring unit), will be automatically selected.

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The hourglass icon will be shown on the display until the reading becomes stable.
When the reading is stable and within range of the selected standard, the CFM functional key is

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displayed.

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eo .i
Press CFM to confirm the calibration point.
.n ls
Press ESC to leave calibration. The instrument will return to the main screen and will memorize the
zero calibration data.
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One point automatic slope calibration

It is suggested to perform the slope calibration in air. Allow the probe tip to dry.
Press CAL. The calibration menu will be displayed. Press DO to select the DO calibration. The 100%
saturation standard (or the 8.26 mg/L standard, according to the currently selected measuring unit),
will be automatically selected.
w .t
w w w

The hourglass icon will be shown on the display until the reading becomes stable.
When the reading is stable and within range of the selected buffer, the CFM functional key is displayed.
w

26
 Press CFM to confirm the calibration point.

DO CALIBRATION PROCEDURE
The instrument will return to the main screen and will memorize the slope calibration data.
Two points automatic calibration
Submerge the probe into HI7040 zero oxygen solution and stir gently for 2-3 minutes.
Press CAL. The calibration menu will be displayed.
Press DO to select the DO calibration. The DO calibration screen will be displayed and the standard
0% saturation (0 mg/L) will be automatically selected.
The hourglass icon will be shown on the display until the reading becomes stable.

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When the reading is stable and within range of the selected standard, the CFM functional key is
displayed.

o.
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Press CFM to confirm the calibration point.
The meter will automatically select the 100% saturation (8.26 mg/L) standard.

n
Gently shake the probe dry. Leave the probe in air.

.c
The hourglass icon will be shown on the display until the reading becomes stable.
When the reading becomes stable within an acceptable range of the selected standard, the CFM
eo .i
functional key is displayed.
Press CFM to confirm the calibration point. The instrument will return to the main screen and will
.n ls
memorize the calibration data.
w oo

One point manual calibration

First determine the dissolved oxygen value of the sample (use a Winkler titration). Place the probe in
the sample and provide adequate stirring.
Access the DO calibration screen as described in the previous DO calibration procedures.
Press the Manual functional key.
w .t

The standard value can be changed using the / keys in the range 0 to 100% saturation or
0.00 to 8.26 mg/L, depending on the currently selected measuring unit.
w w w
w

Set the standard value using the / keys to the determined DO value.
The hourglass icon will be shown on the display until the reading becomes stable.
When the reading is stable and within range of the selected standard, the CFM functional key is
displayed

27
 DO CALIBRATION PROCEDURE

Press CFM to confirm the calibration point.

The instrument will return to the main screen and will memorize the calibration data.
Notes: If the manual pressure feature is enabled, during the DO calibration it is possible

cs h
to switch between changing the standard value or the pressure value by pressing the

t
Pressure/Standard functional key or the MODE key.

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.c
eo .i
If a previous calibration has been performed it is possible to erase the calibration by pressing
the Clear functional key while in the DO calibration screen.
.n ls
w oo

The “Calibration cleared” message will be displayed for a few seconds and the meter will
return to the main screen. If the manual pressure feature is enabled, the Clear key will be
w .t

active only for 5 seconds after accessing the DO calibration screen, and afterwards it will be
replaced by the Pressure/Standard functional key.
w w

If the DO value measured by the instrument is not within range of the selected standard,
the “Wrong standard” error message will be shown on the display and the calibration can’t
be confirmed.
w
w

While in manual calibration mode it is possible to return to the automatic calibration mode
by pressing the AUTO functional key. The meter will select the standard that is closest to the
current DO reading.

28
 GLP is a set of functions that allows storage and retrieval of data regarding the maintenance and

GOOD LABORATORY PRACTICE (GLP)

status of the electrode.
All data regarding DO calibration is stored for the user to review when necessary.

EXPIRED CALIBRATION
The calibration time out can be set (see SETUP for details, page 30) from 1 to 7 days or can be disabled.
The instrument is provided with a real time clock (RTC) in order to monitor the time elapsed since

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the last DO calibration.

o.
The real time clock is reset every time the instrument is calibrated and the “Expired Calibration”

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status is triggered when the instrument detects a calibration time out. The “CAL DUE” tags will start
blinking to warn the user that the instrument should be recalibrated.

n
.c
For example, if a 4 days time out has been selected, the instrument will issue the alarm exactly 4
days after the last calibration.
eo .i
However, if at any moment the expiration value is changed (e.g. to 5 days), then the alarm will be
immediately recalculated and appear 5 days after the last calibration.
.n ls
Notes: When the instrument is not calibrated or the calibration is cleared (default values loaded)
there is no “Expired Calibration”, and the display always shows the “CAL DUE” tags blinking.
When an abnormal condition in the RTC is detected, the instrument forces the “Expired
w oo

Calibration” status.

LAST DO CALIBRATION DATA

Data from the last DO calibration is stored automatically after a successful calibration. To view the last
w .t

calibration data, press GLP when the instrument is in the DO, BOD, OUR or SOUR measurement mode.
w w w

The instrument will display GLP data related to calibration including calibration standard, salinity,
w

pressure and temperature.

Note: “No user calibration” message is displayed if the calibration was cleared or the instrument
hasn’t been calibrated for dissolved oxygen.

29
 Setup mode allows viewing and modifying the measurement parameters.
SETUP

The following table lists the SETUP parameters, their valid range and the factory default settings.

Item Description Valid value Default

Calibr. Time-out Number of days after Disabled, 1 to 7 Disabled
calibr. warning is displayed days
Salinity The solution’s salt content 0 to 70 g/L 0 g/L

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BOD Configuration

o.
Sample min delta The minimum diff. between the 0.00 to 50.00 mg/L 0.00 mg/L

ni .t
DO start and the end DO val.
Sample min end DO The minimum end DO value 0.00 to 50.00 mg/L 0.00 mg/L

n
Seed min delta DO The minimum diff. between the 0.00 to 50.00 mg/L 0.00 mg/L

.c
start and the end DO val.
eo .i
Seed min end DO The minimum end DO value 0.00 to 50.00 mg/L 0.00 mg/L
OUR Configuration
.n ls
Min time The minimum time for the OUR test 1 to 3600 sec. 1 sec.
Max time The maximum time for the OUR test 1 to 3600 sec. 3600 sec.
Min start DO The minimum DO value for starting 0.01 to 50.00 mg/L 0.01 mg/L
w oo

the OUR test

Min end DO The minimum DO value at the end 0.00 to 50.00 mg/L 0.00 mg/L
of the OUR test
Total volume The total volume of the solution to 0.1 to 300.0 mL 0.1 mL
be tested
w .t

Sample volume The volume of sample in the 0.1 to 300.0 mL 0.1 mL

solution to be tested
w w

SOUR configuration
Min time The minimum time for the SOUR 1 to 3600 sec. 1 sec.
test
w

Max time The maximum time for the SOUR 1 to 3600 sec. 3600 sec.
test
w

Min start DO The minimum DO value for starting 0.01 to 50.00 mg/L 0.01 mg/L
the SOUR test
Min end DO The minimum DO value at the end 0.00 to 50.00 mg/L 0.00 mg/L
of the test

30
 SETUP
Total volume The total volume of the solution to 0.1 to 300.0 mL 0.1 mL
be tested
Sample volume The volume of sample in the 0.1 to 300.0 mL 0.1 mL
solution to be tested
Solids weight Total solids or Volatile Suspended 0.1 to 300.0 g/L 0.1 g/L
solids weight
SOUR @ 20 °C Correct the SOUR value to 20 °C Enabled or Disabled

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Disabled

o.
Autodelete BOD Automatically delete BOD start Enabled or Disabled

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start data data, after BOD compute Disabled
Manual pressure Set the pressure value using Enabled or Disabled

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the / keys disabled
Pressure unit mmHg, inHg, mmHg
eo .i
atm, mbar, psi,
kPa
.n ls
Temperature unit °C or °F °C
Backlight Backlight Level 0 to 7 4
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Contrast Contrast Level 0 to 20 10

Auto light off Time backlight remains ON 1, 5, 10, 30 1
Auto power off Time until instrument is powered Disabled 30
OFF 5, 10, 30, 60
w .t

Date / Time 01.01.2006 to current date/time

12.31.2099
w w

00 :00 to 23 :59
Time format AM/PM or 24 24 hours
hours
w

Date Format DD/MM/YYYY YYYY/MM/DD

YYYY/MM/DD
w

MM/DD/YYYY
YYYY/MM/DD
YYYY-MM-DD
Mon DD, YYYY
DD-Mon-YYYY
31
 SETUP

Language Message display language Up to 3 languages English

Beep ON Beeper Status Enabled or Disabled Disabled
Instrument ID Instrument identification 0000 to 9999 0000
Baud Rate Serial Communication to PC 600, 1200, 2400, 4800, 9600
9600
Meter Information Displays general information

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PARAMETER SCREENS

o.
Calibration Timeout

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Highlight Calibration timeout.

n
.c
eo .i
.n ls
Press Modify.
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Use the / keys to set the desired value.

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Press Accept to confirm or ESC to return without saving.

Note: If enabled “CAL DUE” warning will be displayed after Calibration timeout period has
w w

elapsed since the previous calibration.

Salinity
Highlight Salinity.
w
w

Press Modify.

32
 SETUP
Use / keys to change the salinity value. Press Accept to confirm or ESC to exit without saving.
BOD Configuration
Highlight BOD configuration.

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Press Select

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eo .i
.n ls
Parameters:
• Sample min ∆ DO -the minimum acceptable difference between the initial and final DO values
w oo

for a sample. If the difference is less than this value the meter will show a warning message when
evaluating the BOD.
Range: 0.00 to 50.00 mg/L.
• Sample min end DO - the minimum acceptable final DO value for a sample. If the final DO value
is less than this value the meter will show a warning message when evaluating the BOD.
w .t

Range: 0.00 to 50.00 mg/L.

• Seed min ∆ DO - the minimum acceptable difference between the initial and final DO values
w w

for a seed sample. If the difference is less than this value the meter will show a warning message
when evaluating the BOD.
Range: 0.00 to 50.00 mg/L.
w

• Seed min end DO - the minimum acceptable final DO value is less than this value the meter will
show a warning message when evaluating the BOD.
Range: 0.00 to 50.00 mg/L.
w

Press Prev/Next to select a different parameter.

Press / keys to modify the selected parameter’s value.
Press Save to save the new BOD configuration.
Press ESC to leave without changing.
33
 OUR Configuration
SETUP

Highlight OUR configuration.

Press Select.

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Parameters:
eo .i
• Min time - the minimum time for the OUR test.
Range: 1 to 3600 seconds.
.n ls
• Max time - the maximum time for the OUR test. The test will stop automatically when the maximum
time has elapsed.
Range: 1 to 3600 seconds.
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• Min start DO - the minimum accepted DO value for starting the OUR test. If the DO reading is
less than this value the test cannot be started.
Range: 0.01 to 50.00 mg/L.
• Min end DO - the minimum accepted DO value at the end of the test. If the DO reading at the
end of the OUR test is less than this value a warning message will be displayed.
w .t

Range: 0.00 to 50.00 mg/L.

• Total volume - the volume of the diluted mixture.
w w

Range: 0.1 to 300.0 mL

• Sample volume - the volume of sample in the diluted mixture.
Range: 0.1 to 300.0 mL.
w

Press Prev/Next to select a different parameter.

Press / keys to modify the selected parameter’s value.
w

Press Save to save the new OUR configuration.

Press ESC to leave without changing.

34
 SOUR Configuration

SETUP
Highlight SOUR configuration.

Press Select.

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.c
• Min time - the minimum time for the SOUR test.
Range: 1 to 3600 seconds.
eo .i
• Max time - the maximum time for the SOUR test. The test will stop automatically when the
maximum time has elapsed.
.n ls
Range: 1 to 3600 seconds.
• Min start DO - the minimum accepted DO value for starting the SOUR test. If the DO reading is
less than this value the test cannot be started.
w oo

Range: 0.01 to 50.00 mg/L.

• Min end DO - the minimum accepted DO value at the end of the test. If the DO reading at the
end of the SOUR test is less than this value a warning message will be displayed.
Range: 0.00 to 50.00 mg/L.
w .t

• Total vol - the volume of the diluted mixture.

Range: 0.1 to 300.0 mL
• Sample vol - the volume of sample in the diluted mixture.
w w

Range: 0.1 to 300.0 mL.

• Solids weight: Total solids or Volatile suspended solids weight.
Range: 0.1 to 300.0 g/L.
w

• SOUR @ 20 °C: If this option to enabled the SOUR value is corrected to 20 °C.
w

Press Prev/Next to select a different parameter.

Press / keys to modify the selected parameter’s value.
Press Save to save the new SOUR configuration.
Press ESC to leave without changing.

35
 Autodelete BOD Start Data
SETUP

Highlight Autodelete BOD start data.

cs h
Press the displayed functional key to enable/disable the feature.

t
If enabled the BOD initial data record used in BOD result evaluation is deleted automatically after the

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BOD result has been saved into the instrument’s memory (LOG key pressed).
If disabled, the user has to delete BOD initial data records that were used in BOD result evaluation,

n
entering View initial BOD data mode.

.c
Manual Pressure
Highlight Manual pressure.
eo .i
.n ls
w oo

Press the displayed functional key to enable/disable the feature.

If enabled, the pressure can be entered by the user, while in measurement screen, using / keys.
Pressure Unit
w .t

Highlight Pressure unit.

w w w

Press Modify.
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36
 Use / keys to highlight the desired pressure unit.

SETUP
Press Accept to confirm or ESC to exit without saving.
Temperature Unit
Highlight Temperature Unit.

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Press the displayed functional key in order to change the temperature unit.
Backlight

n
Highlight Backlight.

.c
eo .i
.n ls

Press Modify.
w oo
w .t

Use / keys to change intensity then press Accept to confirm.

w w

Press ESC to leave without changing.

Contrast
Highlight Contrast.
w
w

Press Modify.

37
 SETUP

Use / keys to change intensity then press Accept to confirm.

Press ESC to leave without changing.

cs h
Auto Light off

t
Highlight Auto Light off.

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.c
eo .i
Press one of the functional keys to change the option.
.n ls
Auto power off
Highlight Auto power off.
w oo
w .t

Press Modify.
w w w

Use / keys to select interval then press Accept.

Press ESC to leave without changing.
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38
 Date/Time

SETUP
Highlight Date / Time.

Press Modify.

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t
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ni .tn
Use / keys to select item. Use / keys to change the focused values.

.c
Press Accept to confirm the new setting, or ESC to leave without changing.
eo .i
Time Format
Highlight Time format.
.n ls
w oo

Press functional keys to change the option.

Date Format
w .t

Highlight Date Format.

w w w

Press Modify.
w

Use / keys to select date format then press Accept.

Press ESC to leave without changing.

39
 Language
SETUP

Highlight Language.

cs h
Use the desired functional key to change the option. Wait until the new language is loaded.

t
If any language can be loaded, the instrument will work in safe mode. In this mode all messages are

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displayed in English and Help is not available.
Beep On

n
Highlight Beep On.

.c
eo .i
.n ls
Press the displayed functional key to enable/disable beep.
w oo

When enabled, the beep sounds as a short beep every time a key is pressed or when the calibration
can be confirmed.
A long beep alerts that the pressed key is not active or a wrong condition is detected while in calibration.
Instrument ID
w .t

Highlight Instrument ID.

w w w

Press Modify.
w

Use / keys to change the instrument’s ID. Press Accept to confirm or ESC to exit without saving.

40
 Baud Rate

SETUP
Highlight Baud Rate.

Press Modify.

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.c
Use / keys to select the desired communication baud. Press Accept to confirm or ESC to exit.
Meter Information
eo .i
Highlight Meter Information.
.n ls
w oo

Press Select.
w .t
w w w

The meter information is displayed:

-Firmware version
-Language version
w

-DO and Temperature factory calibration date/time

-Battery Capacity

41
 This feature allows the user to log DO, BOD, OUR and SOUR measurements. All logged data can be
LOGGING

transferred to a PC through the USB port using HI92000 application.

The maximum logging space is 400 records.
LOGGING THE CURRENT DATA
To store the current reading into memory, press LOG.

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The instrument will display for a few seconds the record number and the amount of the free log

n
space (in %).

.c
If the LOG space is full, the “Log space is full” message will be displayed for a few seconds when
eo .i
Log key is invoked.
.n ls
w oo

Enter View Logged Data mode and delete records in order to free log space.
VIEW LOGGED DATA
Press RCL to retrieve the stored information while in measurement mode for the specific range (DO,
BOD, OUR, SOUR).
w .t

The list of records is displayed.

DO recall:
w w w

BOD recall:
w

42
 OUR recall:

LOGGING
SOUR recall:

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o.
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If there is no logged data, the instrument will display “No Records”.
Use / keys to scroll the list of records.

n
.c
Press Delete All to enter Delete All screen.
eo .i
Press Delete to enter Delete records screen.
Press More to view more information of the focused record.
.n ls
If More is pressed, a complete set of information is displayed.
Press Pg Up or Pg Down to toggle between information screens.
DO recall:
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BOD recall:
w w w
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43
 Note: “S.C. “ message in the title bar means seed corrected.
LOGGING

“not S.C.” message in the title bar means seed not corrected.

cs h
The Correct functional key will be displayed if the BOD result was not seed corrected.

t
Note: For a seed corrected sample, the last page will show the Seed bottle ID used for correction.

o.
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OUR recall:

n
.c
eo .i
.n ls
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SOUR recall:
w .t
w w w
w

Note: In case that the SOUR value was corrected to 20 °C the message “(@20 °C)” will
be displayed before the SOUR value.

44
 LOGGING
Use / keys to view the complete log information about the next/previous record while are
displayed.

cs h
If Delete is pressed.

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o.
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.c
eo .i
Use / key to highlight the record to be deleted and then press CFM.
.n ls
Press ESC to exit.
If Delete All is pressed the instrument asks for confirmation. Press CFM to confirm or ESC to exit
without deleting.
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45
 To freeze the first stable reading on the LCD press AutoEnd while the instrument is in DO measurement
AUTOEND

mode.
The “Wait” symbol will blink until the reading is stable.

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When the reading is stable “Hold” icon will be displayed.

n
.c
eo .i
.n ls
Press Continue at any moment in order to enter continuous reading mode.
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46
 The HI98193 meter has an internal barometer for automatic pressure compensation for DO readings.

PRESSURE CALIBRATION
The instrument is factory calibrated for pressure measurements and no user calibration is needed. If
the pressure reading is inaccurate, a pressure calibration should be performed.
For an accurate calibration follow the instructions below.
In order to perform a pressure calibration a reference barometer with at least 1 mmHg resolution is
necessary.
Press CAL from any measure mode (DO, BOD, OUR or SOUR). The calibration menu will be displayed.

cs h
t
o.
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.c
eo .i
Press Pressure functional key to select the pressure calibration. The pressure calibration screen will
be displayed.
.n ls
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Using the / keys, enter the true local barometric pressure read from the reference barometer. Do
NOT use the pressure reported by the weather bureau. Weather bureaus correct pressures to sea level.
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When the reading is stable within range of the entered barometric pressure the CFM functional key
is displayed.
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w

Press CFM to confirm the calibration.

The instrument will return to the main screen and will memorize the calibration data.

47
 Note: If a pressure calibration has been previously performed it is possible to erase the calibration
PRESSURE CALIBRATION

by pressing the Clear functional key.

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The “Calibration cleared” message will be displayed for a few seconds and the meter will

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return to the main screen.
If the measured pressure is too far from the calibration point the “Wrong pressure” error

n message will be shown on the display and the calibration can’t be confirmed.

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Verify if the value read from the reference barometer was entered correctly.
Contact the Hanna Service Department if calibration cannot be performed.
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48
 TEMPERATURE CALIBRATION (for technical personnel only)
All the instruments are factory calibrated for temperature.
Hanna’s DO probes are interchangeable and no temperature calibration is needed when they are
replaced.
If the temperature measurements are inaccurate, temperature recalibration should be performed.
For an accurate recalibration follow instructions below.
The temperature calibration can be performed in one or two points.
It is better to perform a two points calibration.
The calibration can be performed in any 2 points that have at least 25 °C distance between. It is

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recommended that the first point be near 0 °C and the second point near 50 °C.

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Press CAL from any measure mode (DO, BOD, OUR or SOUR).

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The calibration menu will be displayed.

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Press the T functional key to select the temperature calibration.
• Prepare a vessel containing ice and water and another one containing hot water (at approximately
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50 °C or 122 °F). Place insulation material around the vessels to minimize temperature changes.
Use a calibrated thermometer with a resolution of 0.1 °C as a reference thermometer.
• Ensure the DO probe is connected to the meter.
• Immerse the DO probe into the vessel with ice and water as close as possible to the reference
thermometer.
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• Allow a few seconds for the probe to stabilize.

w w w

• Use the / keys to set the calibration point values to that of the ice and water mixture,
measured by the reference thermometer.
w

49
 • When the reading is stable and within range of the selected calibration point, the CFM functional
TEMPERATURE CALIBRATION (for technical personnel only)

key is displayed.

• Press CFM to confirm.

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• The second expected calibration point is displayed.

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• Immerse the DO probe into the second vessel as close as possible to the reference thermometer.
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• Allow a few seconds for the probe to stabilize.
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• Use the / key to set the calibration point value to that measured by the reference thermometer.
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• When the reading is stable and within range of the selected calibration point, the CFM functional
key is displayed.
w
w

• Press CFM to confirm. The instrument will return to the main screen.

50
 Note: If the reading is not within range of the selected calibration point or the difference

TEMPERATURE CALIBRATION (for technical personnel only)

between first selected point and second selected point is less than 25 °C, “Wrong” message
will blink.

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t
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If the cause of “Wrong“ message is the difference between calibration points increase the

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temperature of the vessel with hot water until the calibration points are more than 25 °C
apart.

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If the cause of “Wrong“ message is the temperature reading value change the probe and
restart calibration.
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If calibration cannot be performed contact Hanna Service.
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If a temperature calibration has been previously performed it is possible to erase the

calibration by pressing the Clear functional key.
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The “Calibration cleared” message will be displayed for a few seconds and the meter will
return to the main screen.
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For one point calibration press ESC after first point was confirmed.
The instrument will return to the main screen and will memorize the calibration data.
w
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51
 Data transmission from the instrument to the PC can be done with the HI92000 Windows® compatible
PC INTERFACE

software (optional). HI92000 also offers graphing and on-line help feature.
Data can be exported to the most popular spreadsheet programs for further analysis.
To connect your instrument to a PC, use a USB cable connector. Make sure that your instrument is
switched off and plug one connector to the instrument USB socket and the other to the serial or USB
port of your PC.
Note: If you are not using Hanna Instruments HI92000 software, please see the following

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instructions.

t
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SENDING COMMANDS FROM PC

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It is also possible to remotely control the instrument with any terminal program. Use a USB cable
to connect the instrument to a PC, start the terminal program and set the communication options as

n follows: 8, N, 1, no flow control.

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COMMAND TYPES
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To send a command to the instrument follow the next scheme:
<command prefix> <command> <CR>
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where: <command prefix> is a selectable 16 ASCII character.
<command> is the command code.
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Note: Either small or capital letters can be used.

SIMPLE COMMANDS
KF1 Is equivalent to pressing functional key 1
KF2 Is equivalent to pressing functional key 2
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KF3 Is equivalent to pressing functional key 3

RNG Is equivalent to pressing RANGE
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MOD Is equivalent to pressing MODE

CAL Is equivalent to pressing CAL
w

UPC Is equivalent to pressing the UP arrow key

DWC Is equivalent to pressing the DOWN arrow key
RCL Is equivalent to pressing RCL
w

SET Is equivalent to pressing SETUP

GLP Is equivalent to pressing GLP
OFF Is equivalent to pressing OFF

52
 CHR xx Change the instrument range according to the parameter value (xx):

PC INTERFACE
• xx=20 DO range
• xx=21 BOD range
• xx=22 OUR range
• xx=23 SOUR range
The instrument will answer for these commands with:
<STX> <answer> <ETX>

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where: <STX> is 02 ASCII code character (start of text)

t
<ETX> is 03 ASCII code character (end of text)

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<answer>:
<ACK> is 06 ASCII code character (recognized command)

n <NAK> is 21 ASCII code character (unrecognized command)

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<CAN> is 24 ASCII code character (corrupted command)
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COMMANDS REQUIRING AN ANSWER
The instrument will answer for these commands with:
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<STX> <answer> <checksum> <ETX>
where the checksum is the bytes sum of the answer string sent as 2 ASCII characters.
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All the answer messages are with ASCII characters.

RAS Causes the instrument to send a complete set of readings in according with the current range:
• DO, temperature, and pressure on the DO and BOD range
• DO, temperature, pressure, OUR/SOUR value, OUR/SOUR test time on the OUR/SOUR range
• BOD result, start DO value and end DO value when in the BO result screen (meter mode 25)
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The answer string contains:

• Meter mode (2 chars):
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- 20 - DO range
- 21 - BOD range
- 22 - OUR range
w

- 23 - SOUR range
- 25 - BOD result screen
• Meter status (2 chars of status byte): represents a 8 bit hexadecimal encoding
w

• 0x10: temperature probe connection

• 0x20: DO measure unit (0 = %, 1= mg/L)
• 0x01: new GLP data available
• 0x02: new setup parameter

53
 • 0x04: out of calibration range
PC INTERFACE

• 0x08: the meter is in autoend mode

• Reading status: R - in range, O - over range, U - under range
• The following status flags are sent for all modes except mode 25
• DO reading range flag
• temperature reading range flag
• pressure reading range flag
• OUR/SOUR reading range flag (sent only if in OUR/SOUR measure mode)

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• The following status flag is sent only for mode 25
• BOD reading range flag

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• Readings
• The following values are sent for all modes except mode 25

n • DO reading, including sign and decimal point (8 chars)

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• temperature, including sign and decimal point (8 chars)
• pressure value, including sign and decimal point, always in mmHg (11 chars)
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• OUR/SOUR reading, including sign and decimal point (sent only if in OUR/SOUR
measure mode) (8 chars)
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• OUR/SOUR counter (sent only if in OUR/SOUR measure mode) (4 chars)
• The following values are sent only for mode 25
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• BOD reading, including sign and decimal point (8 chars)

• initial DO value, including sign and decimal point [mg/L] (6 chars)
• final DO value, including sign and decimal point [mg/L] (6 chars)
MDR Requests the instrument model name and firmware code (16 ASCII chars).
GLP Requests the calibration data record.
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The answer string contains:

• the number of calibrated buffers (1 char)
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• calibrated buffer unit (0 = %, 1 = mg/L) (1 char)

• buffer value including sign and decimal point (6 chars)
• calibrated buffer unit (0 = %, 1 = mg/L) (this value is sent only if there is a two point
w

calibration) (1 char)
• buffer value including sign and decimal point (this value is sent only if there is a two point
calibration) (6 chars)
w

• salinity value (3 chars)

• pressure value in mmHg, including sign and decimal point (11 chars)
• temperature value including sign and decimal point (8 chars)
• calibration time: yymmddhhmmss (12 chars).

54
 PAR Requests the setup parameters setting.

PC INTERFACE
The answer string contains:
• backlight value (1 char)
• contrast value (2 chars)
• instrument ID (4 chars)
• calibration alarm timeout (2 chars)
• Setup information (2 chars): 8 bit hexadecimal encoding:
• 0x01: - beep is on

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• 0x04: - Celsius / Fahrenheit display (°C if the bit is set)

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• 0x10: - manual pressure (1 if activated, 0 otherwise)

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• Auto Light Off time (3 chars)
• Auto Power Off time (3 chars)

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• Salinity value (3 chars)

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• Pressure unit (1 char): 0 - mmHg, 1 - inHg, 2 - atm, 3 - mbar, 4 - psi, 5 - kPa
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• BOD configuration values
• sample minimum delta DO, including sign and decimal point (6 chars)
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• sample minimum end DO, including sign and decimal point (6 chars)
• seed minimum delta DO, including sign and decimal point (6 chars)
• seed minimum end DO, including sign and decimal point (6 chars)
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• OUR configuration values

• minimum time in seconds (4 chars)
• maximum time in seconds (4 chars)
• minimum start DO including sign and decimal point (6 chars)
• minimum end DO including sign and decimal point (6 chars)
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• total volume including sign and decimal point (6 chars)

• sample volume including sign and decimal point (6 chars)
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• SOUR configuration values

• minimum time in seconds (4 chars)
• maximum time in seconds (4 chars)
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• minimum start DO including sign and decimal point (6 chars)

• minimum end DO including sign and decimal point (6 chars)
• total volume including sign and decimal point (6 chars)
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• sample volume including sign and decimal point (6 chars)

• solids weight including sign and decimal point (6 chars)
• SOUR temperature correction (1=enabled, 0=disabled) (1 char)
• The short name of the selected language (3 chars)

55
 NSLx Requests the number of logged samples (4 chars)
PC INTERFACE

The command parameter (x - 1 char)

• D - the request is for DO
• B - the request is for BOD
• O - the request is for OUR
• S - the request is for SOUR
• I - the request is for BOD initial values
LODDxxx Requests the xxxth DO logged data

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LODBxxx Requests the xxxth BOD logged data
LODOxxx Requests the xxxth OUR logged data

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LODSxxx Requests the xxxth SOUR logged data
LODIxxx Requests the xxxth initial BOD value logged data

n LODDALL Requests all the DO log on demand

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LODBALL Requests all the BOD log on demand
LODOALL Requests all the OUR log on demand
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LODSALL Requests all the SOUR log on demand
LODIALL Requests all the initial BOD values log
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The answer string for each record contains:
• The logged mode (2 chars)
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• 20 - DO range
• 21 - BOD range
• 22 - OUR range
• 23 - SOUR range
• 24 - BOD initial values
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• DO log data:
• Measurement unit (0 = %, 1 = mg/L) (1 char)
w w

• DO value including sign and decimal point (8 chars)

• Salinity value [g/L] (3 chars)
• Pressure value in mmHg, including sign and decimal point (11 chars)
w

• Temperature value including sign and decimal point (8 chars)

• BOD log data:
• Sample type (1 = sample, 0 = seed) (1 char)
w

• Seed corrected (1 = corrected, 0 = not corrected) (1 char)

• Bottle ID (4 chars)
• BOD value including sign and decimal point [mg/L] (8 chars)
• Bottle volume including sign and decimal point [ml] (6 chars)

56
 • Sample volume including sign and decimal point [ml] (6 chars)

PC INTERFACE
• Seed volume including sign and decimal point [ml] (6 chars)
• Start salinity value [g/L] (3 chars)
• End salinity value[g/L] (3 chars)
• Start pressure value in mmHg, including sign and decimal point (11 chars)
• End pressure value in mmHg, including sign and decimal point (11 chars)
• Start temperature value, including sign and decimal point (8 chars)
• End temperature value, including sign and decimal point (8 chars)

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• Start DO value including sign and decimal point [mg/L] (8 chars)
• End DO value including sign and decimal point [mg/L] (8 chars)

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• Seed ID (for seed corrected samples) (4 chars)
• OUR log data:

n
• Start DO value including sign and decimal point [mg/L] (8 chars)

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• End DO value including sign and decimal point [mg/L] (8 chars)
• Salinity value [g/L] (3 chars)
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• Start pressure value in mmHg, including sign and decimal point (11 chars)
• End pressure value in mmHg, including sign and decimal point (11 chars)
.n ls
• Start temperature value, including sign and decimal point (8 chars)
• End temperature value, including sign and decimal point (8 chars)
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• Total volume, including sign and decimal point [ml] (6 chars)

• Sample volume, including sign and decimal point [ml] (6 chars)
• Delta time [s] (4 chars)
• OUR value, including sign and decimal point [mg/L/h] (8 chars)
• SOUR log data:
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• Start DO value including sign and decimal point [mg/L] (8 chars)

• End DO value including sign and decimal point [mg/L] (8 chars)
w w

• Salinity value [g/L] (3 chars)

• Start pressure value in mmHg, including sign and decimal point (11 chars)
• End pressure value in mmHg, including sign and decimal point (11 chars)
w

• Start temperature value, including sign and decimal point (8 chars)

• End temperature value, including sign and decimal point (8 chars)
• Total volume, including sign and decimal point [ml] (6 chars)
w

• Sample volume, including sign and decimal point [ml] (6 chars)

• Delta time [s] (4 chars)
• SOUR value, including sign and decimal point [mg/h/g] (8 chars)
• Solids weight, including sign and decimal point [g/L] (6 chars)

57
 • SOUR temperature correction (1=SOUR@20°C, 0=SOUR not corrected) (1 char)
PC INTERFACE

• BOD DATA log data:

• Sample type (1 = sample, 0 = seed) (1 char)
• Bottle ID (4 chars)
• DO value, including sign and decimal point [mg/L] (8 chars)
• Bottle volume, including sign and decimal point [ml] (6 chars)
• Sample volume, including sign and decimal point [ml] (6 chars)
• Seed volume, including sign and decimal point [ml] (6 chars)

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• Salinity value [g/L] (3 chars)
• Pressure value in mmHg, including sign and decimal point (11 chars)

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• Temperature value, including sign and decimal point (8 chars)
• Log time: yy mm dd hh mm ss (12 chars)

.c
Notes: “Err8” is sent if the instrument is not in measurement mode.
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“Err6” is sent if the requested range is not available.
“Err4” is sent if the requested set parameter is not available.
.n ls
“Err3” is sent if the Log on demand is empty.
“Err9” is sent if the battery power is less than 30%.
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Invalid commands will be ignored.

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w

58
 To replace the batteries, follow the next steps:

BATTERIES REPLACEMENT
• Turn OFF the instrument.
• Open the battery compartment by removing the four screws from the back of the instrument.
• Remove the old batteries.
• Insert four new 1.5V AA batteries in the battery compartment while paying attention to the correct
polarity.
• Close the battery compartment using the four screws.

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If the battery capacity is less than 20 % the serial communication and the backlight feature are not

t
available.

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Note: The instrument is provided with the BEPS (Battery Error Prevention System) feature,
w

which automatically turns the instrument off when the batteries level is too low to ensure
reliable readings.
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59
 The oxygen probe is made of reinforced plastic for maximum durability.
PROBE MAINTENANCE

A thermistor temperature sensor provides temperature measurements

of the sample. Use the protective cap when not in use.
To replace the membrane or refill with electrolyte, proceed as follows:
Remove the protective cap by gently twisting, and pulling it off the
body of the probe (see fig. 1).
Unscrew the membrane cap by turning it counterclockwise (see fig. 2).

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Wet the sensor by soaking the bottom 21/2 cm (1’’) of the probe in

t
electrolyte for five minutes.

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Rinse the new membrane cap, supplied with the meter with electrolyte
solution while shaking it gently. Refill with clean electrolyte solution.

n Gently tap the sides of the membrane cap with your finger tip to ensure

.c
that no air bubbles remain trapped. Do not tap directly the bottom
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with your finger, as this will damage the membrane.
Make sure that the rubber O-Ring sits properly inside the membrane
.n ls
cap. With the sensor facing down, slowly screw the membrane cap
clockwise. Some electrolyte will overflow.
The Platinum cathode (#8 in the Probe Functional Description page 9)
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should always be bright and untarnished. If it is tarnished or stained,

the cathode should be cleaned. You can use a clean lint-free paper
towel or cloth. Rub the cathode very gently side to side 4-5 times. This
will be enough to polish and remove any stains without damaging the
w .t

platinum tip. Afterwards, rinse the probe with deionized or distilled

water and install a new membrane cap using fresh electrolyte and
w w

follow the steps above. Recalibrate the instrument.

w

Important
In order to have accurate and stable measurements, it is important that the membrane surface is in
perfect condition. This semipermeable membrane isolates the sensor elements from the environment
w

but allows oxygen to enter. If any dirt is observed on the membrane, rinse carefully with distilled or
deionized water. If any imperfections still exist, or any damage is evident (such as wrinkles or tears-holes),
the membrane should be replaced.
Make sure that the O-Ring sits properly in the membrane cap.

60
 TROUBLESHOOTING GUIDE
SYMPTOMS PROBLEM SOLUTION

Reading fluctuates up DO probe not properly Connect the probe.

and down (noise). connected.

Display shows DO Reading out of range. Recalibrate the meter;

reading blinking. Check the sample is

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within measurable

o.
range.

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Meter fails to calibrate Broken DO probe. Replace the probe.

n
.c
or gives faulty
readings.
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At startup the meter One of the keys is Check the keyboard
.n ls
displays Hanna logo blocked. or contact your local
permanently. office.
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“Err xx” message Internal error. Contact your local

displayed at startup. office or any Hanna
Service Center.

Meter shuts off. Dead accumulators, Recharge accumu‑

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Auto Power Off feature lators or replace

is enabled: in this accumulator; Press
w w

case, meter shuts off ON/OFF.

after selected period of
non use.
w

The instrument does Initialization error. Press and hold down

not start when ON/OFF for about 20
w

pressing ON/OFF. seconds or disconnect

and then connect one
accumulator.

61
 ACCESSORIES

Code Description
HI98501 Digital thermometer (range: -50.0 to 150.0 °C/-58.0 to 302 °F)
HI7040L Zero Oxygen Solution
HI7041S Refilling Electrolyte Solution (30 mL)
HI764073 Spare probe with 4 meter (13.4’) cable

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HI76407A/P Spare membranes (5 pcs.)

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HI92000 Windows® compatible software application
HI920015 Micro USB cable

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62
 Recommendations Before using Hanna products, make sure that they are entirely suit‑
able for your specific application and for the environment in which
for users they are used. Operation of these instruments may cause unaccept‑
able interferences to other electronic equipment. Take all necessary
steps to correct such interferences.
During operation, ESD wrist straps should be worn to avoid possible
damage to the electrode by electrostatic discharges.
Any variation introduced by the user to the supplied

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t
equipment may degrade the instruments’ EMC performance.
To avoid electrical shock, do not use these instruments when voltages

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at the measurement surface exceed 24 Vac or 60 Vdc.
To avoid damage or burns, do not perform any measurement in

n microwave ovens.

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Warranty The HI98193 is warranted for two years against defects in workmanship and materials
when used for their intended purpose and maintained according to instructions.
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Electrodes and probes are guaranteed for six months. This warranty is limited to
repair or replacement free of charge.
Damage due to accidents, misuse, tampering or lack of prescribed maintenance is
not covered.
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If service is required, contact the dealer from whom you purchased the instrument.
If under warranty, report the model number, date of purchase, serial number and
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the nature of the problem. If the repair is not covered by the warranty, you will be
notified of the charges incurred. If the instrument is to be returned to Hanna Instru‑
ments, first obtain a Returned Goods Authorization (RGA) number from the Technical
w

Service department and then send it with shipping costs prepaid. When shipping any
instrument, make sure it is properly packed for complete protection.
w

Hanna Instruments reserves the right to modify the design, construction or appearance of its
products without advanced notice.
 World
Headquarters

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Hanna Instruments Inc.
Highland Industrial Park

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584 Park East Drive
Woonsocket, RI 02895 USA
www.hannainst.com

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Local Office
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Hanna Instruments USA
270 George Washington Highway
Smithfield, RI 02917
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Phone: 800.426.6287
Fax: 401.765.7575
e-mail: [email protected]
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MAN98193 03/16 Printed in ROMANIA