BC-2800Vet Operator's Manual 12.0 EN H-2810-20-47202 ECM12.0 PDF

BC-2800Vet Auto Hematology Analyzer
Operator’s Manual
© 2005-2010 Shenzhen Mindray Bio-medical Electronics Co., Ltd. All rights Reserved.
For this Operator’s Manual, the issued date is 2010-09.
Intellectual Property Statement
SHENZHEN MINDRAY BIO-MEDICAL ELECTRONICS CO., LTD. (hereinafter called

Mindray) owns the intellectual property rights to this Mindray product and this manual. This
manual may refer to information protected by copyright or patents and does not convey any
license under the patent rights or copyright of Mindray, or of others.
Mindray intends to maintain the contents of this manual as confidential information.

Disclosure of the information in this manual in any manner whatsoever without the written
permission of Mindray is strictly forbidden.
Release, amendment, reproduction, distribution, rental, adaptation, translation or any other
derivative work of this manual in any manner whatsoever without the written permission of
Mindray is strictly forbidden.
, , are the trademarks, registered or otherwise, of Mindray in

China and other countries. All other trademarks that appear in this manual are used only for
informational or editorial purposes. They are the property of their respective owners.
Responsibility on the Manufacturer Party
Contents of this manual are subject to change without prior notice.
All information contained in this manual is believed to be correct. Mindray shall not be liable
for errors contained herein or for incidental or consequential damages in connection with the
furnishing, performance, or use of this manual.
Mindray is responsible for the effects on safety, reliability and performance of this product,
only if:
all installation operations, expansions, changes, modifications and repairs of this product
are conducted by Mindray authorized personnel;
the electrical installation of the relevant room complies with the applicable national and
local requirements; and
the product is used in accordance with the instructions for use.
I
z It is important for the hospital or organization that employs this equipment
to carry out a reasonable service/maintenance plan. Neglect of this may
result in machine breakdown or personal injury.
z Be sure to operate the analyzer under the situation specified in this manual;
otherwise, the analyzer will not work normally and the analysis results will
be unreliable, which would damage the analyzer components and cause
personal injury.
z This equipment must be operated by skilled/trained clinical professionals.
II
Warranty
THIS WARRANTY IS EXCLUSIVE AND IS IN LIEU OF ALL OTHER WARRANTIES,

EXPRESSED OR IMPLIED, INCLUDING WARRANTIES OF MERCHANTABILITY OR
FITNESS FOR ANY PARTICULAR PURPOSE.
Exemptions
Mindray's obligation or liability under this warranty does not include any transportation or
other charges or liability for direct, indirect or consequential damages or delay resulting from
the improper use or application of the product or the use of parts or accessories not approved
by Mindray or repairs by people other than Mindray authorized personnel.
This warranty shall not extend to:
Malfunction or damage caused by improper use or man-made failure.
Malfunction or damage caused by unstable or out-of-range power input.
Malfunction or damage caused by force majeure such as fire and earthquake.
Malfunction or damage caused by improper operation or repair by unqualified or

unauthorized service people.
Malfunction of the instrument or part whose serial number is not legible enough.
Others not caused by instrument or part itself.
III
Company Contact
Manufacturer: Shenzhen Mindray Bio-Medical Electronics Co., Ltd.
E-mail Address: [email protected]
Tel: +86 755 26582479 26582888
Fax: +86 755 26582934 26582500
EC-Representative: Shanghai International Holding Corp. GmbH(Europe)
Address: Eiffestraβe 80, Hamburg 20537, Germany
Tel: 0049-40-2513175
Fax: 0049-40-255726
IV
Table of Contents
1 Using This Manual ................................................................................... 1-1

1.1 Introduction ............................................................................................ 1-1
1.2 Who Should Read This Manual ............................................................. 1-2
1.3 How to Find Information......................................................................... 1-3
1.4 Conventions Used in This Manual ......................................................... 1-4
1.5 Special Terms Used in This Manual....................................................... 1-5
1.6 Symbols ................................................................................................. 1-6
2 Understanding Your Analyzer................................................................. 2-1

2.1 Introduction ............................................................................................ 2-1
2.2 Intended Use.......................................................................................... 2-2
2.3 User Interface......................................................................................... 2-4
2.3.1 LCD ............................................................................................. 2-7
2.3.2 Input Devices .............................................................................. 2-7
2.3.3 Recorder...................................................................................... 2-9
2.3.4 Keyboard Interface...................................................................... 2-9
2.3.5 Serial Ports.................................................................................. 2-9
2.3.6 Parallel Port................................................................................. 2-9
2.3.7 Power Supply for the Floppy Disk Drive ..................................... 2-9
2.3.8 Power Indicator ........................................................................... 2-9
2.3.9 Optional Devices ......................................................................... 2-9
2.4 Instrument Software ............................................................................. 2-10
2.4.1 Main Screen .............................................................................. 2-10
2.4.2 Screen saver ............................................................................. 2-11
2.4.3 System Menu ............................................................................ 2-12
2.5 Reagents, Controls and Calibrators..................................................... 2-14
2.5.1 Diluent ....................................................................................... 2-14
2.5.2 Lyse ........................................................................................... 2-15
2.5.3 Rinse ......................................................................................... 2-15
2.5.4 E-Z Cleanser ............................................................................. 2-15
2.5.5 Probe Cleanser ......................................................................... 2-15
2.5.6 Controls and Calibrators ........................................................... 2-15
3 Understanding the System Principles ................................................... 3-1

3.1 Introduction ............................................................................................ 3-1
3.2 Aspiration ............................................................................................... 3-2
3.3 Dilution ................................................................................................... 3-3
1
Table of Contents
3.4 WBC/HGB Measurement....................................................................... 3-4

3.4.1 Volumetric Metering .................................................................... 3-4
3.4.2 Measurement Principles.............................................................. 3-5
3.4.3 Derivation of WBC-Related Parameters ..................................... 3-6
3.4.4 HGB............................................................................................. 3-7
3.5 RBC/PLT Measurement ......................................................................... 3-8
3.5.1 Volumetric Metering .................................................................... 3-8
3.5.2 Measurement Principles.............................................................. 3-9
3.5.3 Derivation of RBC-Related Parameters ...................................... 3-9
3.5.4 Derivation of PLT-Related Parameters ..................................... 3-10
3.6 Wash .................................................................................................... 3-12
4 Installing Your Analyzer .......................................................................... 4-1

4.1 Introduction ............................................................................................ 4-1
4.2 Installation Requirements....................................................................... 4-2
4.2.1 Space Requirements................................................................... 4-2
4.2.2 Power Requirements................................................................... 4-2
4.2.3 General Environment .................................................................. 4-3
4.3 Unpacking .............................................................................................. 4-4
4.3.1 Unpacking and Inspecting the Analyzer...................................... 4-4
4.3.2 How to move the analyzer........................................................... 4-4
4.4 Installation Procedure ............................................................................ 4-5
4.4.1 Connecting Regents.................................................................... 4-5
4.4.2 Installing Recorder Paper............................................................ 4-7
4.4.3 Connecting the Keyboard............................................................ 4-8
4.4.4 Connecting the Printer (Optional) ............................................... 4-9
4.4.5 Connecting the Bar-Code Scanner (Optional) ............................ 4-9
4.5 Starting the Analyzer ............................................................................ 4-10
5 Customizing the Analyzer Software ....................................................... 5-1

5.1 Introduction ............................................................................................ 5-1
5.2 Password ............................................................................................... 5-2
5.2.1 Entering the administrator password .......................................... 5-2
5.2.2 Resuming the common user password....................................... 5-3
5.3 Editing Settings ...................................................................................... 5-4
5.3.1 Reagent....................................................................................... 5-5
5.3.2 Printing and Communication Settings......................................... 5-7
5.3.3 Date and Time........................................................................... 5-11
5.3.4 Gain........................................................................................... 5-13
5.3.5 Count......................................................................................... 5-16
5.3.6 Reference Range ...................................................................... 5-19
5.3.7 Other Settings ........................................................................... 5-21
5.3.8 Special Fluidic Settings (for user-defined animals)................... 5-24
2
Table of Contents
6 Operating Your Analyzer ......................................................................... 6-1

6.1 Introduction ............................................................................................ 6-1
6.2 Initial Checks.......................................................................................... 6-2
6.3 Power-on................................................................................................ 6-3
6.4 Daily Quality Control .............................................................................. 6-4
6.5 Selecting Animal .................................................................................... 6-5
6.6 Define an animal .................................................................................... 6-6
6.7 Sample Collection and Handling............................................................ 6-7
6.7.1 Whole Blood Samples................................................................. 6-7
6.7.2 Prediluted Samples ..................................................................... 6-7
6.8 Running Whole Blood Samples ........................................................... 6-10
6.8.1 Entering Sample Information .................................................... 6-10
6.8.2 Running the Samples................................................................ 6-14
6.8.3 Special Functions ...................................................................... 6-15
6.9 Running Prediluted Samples ............................................................... 6-17
6.9.1 Entering Sample Information .................................................... 6-17
6.9.2 Running the Samples................................................................ 6-21
6.9.3 Special Functions ...................................................................... 6-22
6.10 Shutdown ............................................................................................. 6-24
7 Reviewing Sample Results ..................................................................... 7-1

7.1 Introduction ............................................................................................ 7-1
7.2 Browsing All Sample Results ................................................................. 7-2
7.2.1 Browsing in the Histogram Mode ................................................ 7-2
7.2.2 Browsing in the “Table” mode...................................................... 7-4
7.3 Searching for Specific Sample Results ................................................ 7-11
7.3.1 Starting a search ....................................................................... 7-11
7.3.2 Reviewing Search Result in the “Table” Mode .......................... 7-12
7.3.3 Reviewing Search Result in the “Histogram” Mode .................. 7-19
8 Using the QC Programs .......................................................................... 8-1

8.1 Introduction ............................................................................................ 8-1
8.2 QC Editing.............................................................................................. 8-2
8.2.1 Entering the “Quality control” screen .......................................... 8-2
8.2.2 Selecting a QC File ..................................................................... 8-2
8.2.3 Editing L-J Settings ..................................................................... 8-3
8.3 Running the Controls ............................................................................. 8-6
8.3.1 Whole Blood Mode...................................................................... 8-6
8.3.2 Prediluted Mode .......................................................................... 8-7
8.4 Reviewing QC Results ......................................................................... 8-10
8.4.1 L-J Graph .................................................................................. 8-10
8.4.2 QC Table ................................................................................... 8-12
9 Using the Calibration Programs ............................................................. 9-1
3
Table of Contents
9.1 Introduction ............................................................................................ 9-1

9.2 When to calibrate ................................................................................... 9-2
9.3 How to Calibrate..................................................................................... 9-3
9.3.1 Preparing Your Analyzer.............................................................. 9-3
9.3.2 Auto Calibration Program............................................................ 9-4
9.3.3 Manual Calibration Program ..................................................... 9-10
10 Maintaining Your Analyzer .................................................................... 10-1

10.1 Introduction .......................................................................................... 10-1
10.2 General Guidelines .............................................................................. 10-2
10.3 Using the “Maintenance” Program ....................................................... 10-3
10.3.1 Diluent Prime............................................................................. 10-4
10.3.2 Rinse Prime............................................................................... 10-5
10.3.3 Lyse Prime ................................................................................ 10-6
10.3.4 Zap Aperture ............................................................................. 10-7
10.3.5 Flush Aperture........................................................................... 10-8
10.3.6 Probe Cleanser Cleaning .......................................................... 10-9
10.3.7 E-Z Cleanser Cleaning............................................................ 10-11
10.3.8 Lyse Test ................................................................................. 10-13
10.3.9 Clean Bath .............................................................................. 10-15
10.3.10 Drain Bath............................................................................. 10-16
10.3.11 Drain Tubing ......................................................................... 10-18
10.3.12 Clean Wipe Block ................................................................. 10-19
10.3.13 Prepare to ship ..................................................................... 10-21
10.4 Using the “Status” Program................................................................ 10-23
10.5 Using the “Self-test” Program ............................................................ 10-24
10.5.1 Testing the fluidic system ........................................................ 10-25
10.5.2 Testing Motors and Recorder/Printer ...................................... 10-25
10.5.3 Testing Valves ......................................................................... 10-26
10.5.4 Testing A/D Interrupt................................................................ 10-27
10.6 Log ..................................................................................................... 10-28
10.7 Viewing System Configuration ........................................................... 10-30
10.8 Printing Management......................................................................... 10-31
10.9 Calibrating Sample Probe Position .................................................... 10-32
10.10 Replacing the Probe Wipe ................................................................. 10-36
10.11 Replacing the Filter of the Vacuum Chamber .................................... 10-37
10.12 Maintaining Recorder ......................................................................... 10-39
11 Troubleshooting Your Analyzer ............................................................ 11-1

11.1 Introduction .......................................................................................... 11-1
11.2 Errors without available error messages ............................................. 11-2
11.3 Errors indicated by error messages ..................................................... 11-3
12 Appendices ............................................................................................ 12-1
4
Table of Contents
A Index ....................................................................................................... 12-1
B Specifications ..........................................................................................B-1
C Precautions, Limitations and Hazards ...................................................C-1
D Communication .......................................................................................D-1
5
1 Using This Manual
1.1 Introduction
This chapter explains how to use your Auto Hematology Analyzer operator’s manual, which is
shipped with your BC-2800Vet auto hematology analyzer and contains reference information
about the BC-2800Vet and procedures for operating, troubleshooting and maintaining the
analyzer. Read this manual carefully before operating your analyzer and operate your
analyzer strictly as instructed in this manual.
 Operate your analyzer strictly as instructed in this manual.
 All illustrations in this manual are provided as examples only. They may not
necessarily reflect your analyzer setup or data displayed and must not be
used for any other purpose.
1-1
Using This Manual
1.2 Who Should Read This Manual
This manual contains information written for clinical laboratory professionals to
 learn about the BC-2800Vet hardware and software;
 customize system settings;
 perform daily operating tasks;
 perform system maintenance and troubleshooting.
1-2
Using This Manual
1.3 How to Find Information
This operator’s manual comprises 11 chapters and 4 appendices. Refer to the table below to
find the information you need.
If you want to … See …

learn about the intended use and parameters of the Chapter 2 Understanding
BC-2800Vet Your Analyzer
learn about the hardware and software of the BC-2800Vet Chapter 2 Understanding
Your Analyzer
learn about how the BC-2800Vet works Chapter 3 Understanding the
System Principles
learn about how to install the BC-2800Vet Chapter 4 Installing Your
Analyzer
learn about how to define/adjust system settings Chapter 5 Customizing the
Analyzer Software
learn about how to use the BC-2800Vet to perform your daily Chapter 6 Operating Your
operating tasks Analyzer
learn about how to review the saved analysis results Chapter 7 Reviewing Sample
Results
learn about how to use the quality control programs Chapter 8 Using the QC
Programs
learn about how to calibrate the BC-2800Vet Chapter 9 Using the
Calibration Programs
learn about how to maintain/service the BC-2800Vet Chapter 10 Maintaining Your
Analyzer
learn about the meanings of the error messages and how to Chapter 11 Troubleshooting
correct the problems Your Analyzer
learn about the technical specifications of the BC-2800Vet Appendix B Specifications
see the summary of all safety messages included in this Appendix C Precautions,
manual Limitations and Hazards
learn about the communication protocol of the BC-2800Vet Appendix D Communication
1-3
Using This Manual
1.4 Conventions Used in This Manual
This manual uses certain typographical conventions to clarify meaning in the text:
 All capital letters enclosed in [ ] indicate a key name (either on the built-in keypad
or the external keyboard), such as [ENTER].
 All capital, bold and italic letters indicate a special operation defined in the
following section, such as SELECT.
 Bold letters included in “ ” indicate text you can find on the screen, such as
“Prepare to ship”.
 Bold letters indicate defined screen areas/fields, such as System Status area, or
chapter titles, such as Chapter 1 Using This Manual.
All illustrations in this manual are provided as examples only. They may not necessarily
reflect your analyzer setup or data displayed.
1-4
Using This Manual
1.5 Special Terms Used in This Manual
When you read … It means …

to press the arrow keys ([←][→] [↑][↓]) as needed to move the
CLICK
cursor to a certain software button on screen and press
[ENTER].
to press the arrow keys ([←][→] [↑][↓]) as needed to move
cursor to the desired edit box and use the built-in keypad or
the external keyboard to enter the desired characters or digits.
ENTER
Note that besides the numeric keys you may also use the
[PgUp] or [PgDn] keys to enter digits; or to scan the number in
using the bar-code scanner.
cursor to the character or digit to the left of the one you want to
delete and press [DEL]; or to press the arrow keys
DELETE
([←][→][↑][↓]) as needed to move the cursor to the character
or digit to the right of the one you want to delete and press
[BackSpace] on the external keyboard.
to move the cursor to the character or digit you want to change
MODIFY and re-enter the desired one using either the built-in keypad or
the external keyboard.
SELECT from “ ** ”
cursor to the desired edit box and press [ENTER] to display
pull-down list
the pull-down list and press [↑] or [↓] to move the cursor to the
desired item and press [ENTER] to select it.
to press the arrow keys ([←][→] [↑][↓]) as needed to the
SELECT
desired item and press [ENTER].
 This analyzer adopts a fixed decimal point. You can enter the digits without
bothering to look for the [.] on the external keyboard.
1-5
Using This Manual
1.6 Symbols
You will find the following symbols in this manual.
When you see… Then…

read the statement below the symbol. The statement is
alerting you to an operating hazard that can cause
personnel injury.
alerting you to a possibility of analyzer damage or unreliable
analysis results.
alerting you to information that requires your attention.
read the statement below the symbol . The statement is

alerting you to a potentially biohazardous condition.
You may find the following symbols on the analyzer or the reagents.
When you see… It means…

EQUIPOTENTIALITY
CAUTION, CONSULT ACCOMPANYING

DOCUMENTS.
BIOLOGICAL RISK
HIGH VOLTAGE
ALTERNATING CURRENT
USE BY
1-6
Using This Manual
SERIAL NUMBER
DATE OF MANUFACTURE
TEMPERATURE LIMITATION
CONSULT INSTRUCTIONS FOR USE
THE DEVICE IS FULLY CONFORMANCE

WITH THE COUNCIL DIRECTIVE
CONCERNING EC LOW VOLTAGE
DIRECTIVE (73/23/EEC) AND EC
DIRECTIVE OF ELECTROMAGNETIC
COMPATIBILITY (2004/108/EC).
THE LABEL APPLIES TO U.S.A ONLY:

FEDERAL LAW RESTRICTS THIS DEVICE
TO SALE BY OR ON THE ORDER OF A
VETERINARIAN.
MANUFACTURER
AUTHORISED REPRESENTATIVE IN THE

EUROPEAN COMMUNITY
IRRITATING SUBSTANCE
THE FOLLOWING DEFINITION OF THE

WEEE LABEL APPLIES TO EU MEMBER
STATES ONLY: THE USE OF THIS SYMBOL
INDICATES THAT THIS PRODUCT SHOULD
NOT BE TREATED AS HOUSEHOLD
WASTE. BY ENSURING THAT THIS
PRODUCT IS DISPOSED OF CORRECTLY,
YOU WILL HELP PREVENT BRINGING
1-7
Using This Manual
POTENTIAL NEGATIVE CONSEQUENCES

TO THE ENVIRONMENT AND HUMAN
HEALTH. FOR MORE DETAILED
INFORMATION WITH REGARD TO
RETURNING AND RECYCLING THIS
PRODUCT, PLEASE CONSULT THE
DISTRIBUTOR FROM WHOM YOU
PURCHASED THE PRODUCT.
1-8
Using This Manual
（4）
（3）
（2）
（1）
Figure 1-1 Back of the analyzer
(1)
Equipotentiality.
（2）
 Connect only to a properly earth grounded outlet;
 To avoid electric shock, disconnect power cord prior to removing or replacing fuse;
 Replace fuse only with the type and rating specified.
（3）
Biological risk.
（4）
The following definition of the WEEE label applies to EU member states only: The use of this
symbol indicates that this product should not be treated as household waste. By ensuring that
1-9
Using This Manual
this product is disposed of correctly, you will help prevent bringing potential negative
consequences to the environment and human health. For more detailed information with
regard to returning and recycling this product, please consult the distributor from whom you
purchased the product.
（5）
Figure 1-2 Warning label
（5）
To avoid being injured, do not put hand under the motor when the machine is running.
1-10
Using This Manual
（6）
Figure 1-3 Warning label
（6）
To avoid electrical shock, disconnect the power supply before maintaining this device.
1-11
Using This Manual
（7）
Figure 1-4 warning label
（7）
High Voltage
1-12
2 Understanding Your Analyzer
2.1 Introduction
The BC-2800Vet Auto Hematology Analyzer is a quantitative, automated hematology

analyzer and leukocyte differential counter for Diagnostic Use in veterinary laboratories.
2-1
Understanding Your Analyzer
2.2 Intended Use
 The purpose of this analyzer is to identify the normal animal patient, with all
normal system-generated parameters, and to flag or identify patient results
that require additional studies.
The analyzer is used for the quantitative determination of a maximum of 18 parameters and 3
histograms of blood samples. See Table 2-1 for details.
White Blood Cell or leukocyte WBC

Lymphocyte Lymph#
Monocyte Mon#
Granulocyte Gran#
Lymphocyte percentage Lymph%
Monocyte percentage Mon%
Granulocyte percentage Gran%
Red Blood Cell or erythrocyte RBC

Hemoglobin Concentration HGB
Mean Corpuscular (erythrocyte) Volume MCV
Mean Cell (erythrocyte) Hemoglobin MCH
Mean Cell (erythrocyte) Hemoglobin Concentration MCHC
Red Blood Cell (erythrocyte) Distribution Width RDW

Hematocrit HCT
Platelet PLT
Mean Platelet Volume MPV
Platelet Distribution Width PDW
Plateletcrit PCT
Eosinophils percentage Eos%
White Blood Cell Histogram WBC Histogram

Red Blood Cell Histogram RBC Histogram
Platelet Histogram PLT Histogram
2-2
Table 2-1 Parameters and animals
Parameter Dog Cat Horse Rat Mouse Rabbit Monkey Cow Pig Buffalo Sheep Camel Goat User-
defined
WBC √ √ √ √ √ √ √ √ √ √ √ √ √ √
Lymph# √ √ √ √ √ √ √ √
Mon# √ √ √ √ √ √ √ √
Gran# √ √ √ √ √ √ √ √
Lymph% √ √ √ √ √ √ √ √
Mon% √ √ √ √ √ √ √ √
Gran% √ √ √ √ √ √ √ √
RBC √ √ √ √ √ √ √ √ √ √ √ √ √ √
HGB √ √ √ √ √ √ √ √ √ √ √ √ √ √
MCV √ √ √ √ √ √ √ √ √ √ √ √ √ √
MCH √ √ √ √ √ √ √ √ √ √ √ √ √ √
MCHC √ √ √ √ √ √ √ √ √ √ √ √ √ √
RDW √ √ √ √ √ √ √ √ √ √ √ √ √ √
HCT √ √ √ √ √ √ √ √ √ √ √ √ √ √
PLT √ √ √ √ √ √ √ √ √ √ √ √
MPV √ √ √ √ √ √ √ √ √ √ √ √
PDW √ √ √ √ √ √ √ √ √ √ √ √
PCT √ √ √ √ √ √ √ √ √ √ √ √
Eos% √ √
WBC √ √ √ √ √ √ √ √ √ √ √ √ √ √
Histogram
RBC √ √ √ √ √ √ √ √ √ √ √ √ √ √
Histogram
PLT √ √ √ √ √ √ √ √ √ √ √ √
Histogram
2-3
2.3 User Interface
Figure 2-1 Front view
1 ---- LCD 2 ---- Keypad

3 ---- Recorder 4 ---- Power indicator
5 ---- Aspirate key 6 ---- Sample probe
2-4
Figure 2-2 Back view
1 --- Power switch 2 --- Equipotentiality

3 --- Waste outlet(Red) 4 --- Lyse inlet(Orange)
5 --- Rinse inlet(Blue) 6 --- Diluent inlet(Green)
2-5
Figure 2-3 Left view
1 --- RS-232 port1 2 --- Parallel port

3 --- RS-232 port2 4 --- Power Interface of Floppy Disk Drive
5 --- Keyboard interface
2-6
2.3.1 LCD
The LCD is located on the front panel of the analyzer, as Figure 2-4 shows. It displays all
alphanumeric and graphic data.
2.3.2 Input Devices

The input devices include the aspirate key, built-in keypad and PS/2 keyboard.
 Aspirate key
The aspirate key is located behind the sample probe, as Figure 2-4 shows. You can press the
key to start the selected run cycle or dispense diluent.
Figure 2-4 Sample probe and aspirate key
1.Sample Probe 2. Aspirate Key
2-7
 Built-in keypad
The 18-key keypad is located below the LCD, as Figure 2-5 shows.
Figure 2-5 Build-in keypad
 PS/2 keyboard
The analyzer can also be controlled by an external PS/2 keyboard that should be connected
to the analyzer’s keyboard interface. See Table 2-2 Key functions for the correspondence
between the keypad keys and the keyboard keys and for their functions.
Table 2-2 Key functions
Keypad PS/2 keyboard Function

[MENU] [Esc] Press it to enter/exit the system menu.
[PRINT] [P] or [p] Press it to print out data by the recorder or printer.
[HELP] [H] or [h] Press it to call out the analyzer’s on-line help.
[DEL] [Delete] or [Del] Press it to delete data and characters.
[ENTER] [Enter] Press it to confirm or execute an operation
[↑], [↓], [↑], [↓], Press them to move the cursor.
[←], [→] [←], [→]
[F1], [F2], [F3], [F1], [F2], [F3], Press them to use various functions. See the rest of this
[F4], [F5] [F4], [F5] manual or the analyzer’s on-line help for details.
[PgUp][PgDn] [PageUp] Press them to scroll the screen.
[PageDown]
[MODE] [Ctrl+A] Switch to another analysis mode (works only in the
Count screen).
[DILUENT] In the prediluted mode, press it if you want to dispense
diluent into the sample tube.
/ Other keys Use them as needed. See the rest of this manual or the
analyzer’s on-line help for details.
2-8
2.3.3 Recorder
A thermal recorder is located on the front panel. It prints out analysis reports and other related
information.
2.3.4 Keyboard Interface

A PS/2 keyboard can be connected here.
2.3.5 Serial Ports

The analyzer provides two RS-232 ports, one for connecting the scanner and the other for
connecting a computer (host).
2.3.6 Parallel Port

The analyzer provides a parallel port to connect a printer or a floppy disk drive (a floppy disk
drive is needed to upgrade the system software; the drive can only be connected by a
Mindray-supplied cable).
2.3.7 Power Supply for the Floppy Disk Drive

It supplies power to the connected floppy disk drive. Only the drive power cable supplied by
Mindraycan be used.
2.3.8 Power Indicator

The power indicator tells you whether the analyzer is on, off or in the screen saver mode.
2.3.9 Optional Devices

 Printer
An external printer can be connected to the parallel port at the left side of the analyzer. You
can use it to print out a detailed report and other related information.
 Bar-code scanner
A bar-code scanner can be connected to the RS-232 port 1 of the analyzer. You can use it to
scan the bar-coded sample IDs into the analyzer.
 Use the printer and scanner of the specified model.
2-9
2.4 Instrument Software
2.4.1 Main Screen

After finishing the startup procedure, the analyzer enters the “Count” screen, which is the
screen to be used most frequently, hence the name “Main screen”, as Figure 2-6 shows.
Title Area Count Mode Area System Status Area
Error Message Help Area Reagent Status

Area Figure 2-6 “Count” screen Area
 Title Area
The Title area displays the title of the current screen.
 Count Mode Area
The Count Mode area displays in which analysis (count) mode, whole blood or prediluted,
the next sample is to be analyzed.
 System Status Area
The System Status area displays whether this analyzer is ready for the next analysis. When
it displays “Ready”, it means this analyzer is ready and you can proceed to analyze the next
sample. When it displays “Waiting”, it means the analyzer is not ready for the next run yet.
When it displays “Running”, it means this analyzer is analyzing a sample.
 System Time Area
2-10
The System Time area displays the system time.
 Sample Information Area
The Sample Information area has two sub-areas, the upper titled “Current sample” and the
lower “Next sample”.
The “Current sample” refers to the sample, whose analysis result is displayed on the
“Count” screen. The animal information (type, name, gender, age), sample ID, time of
analysis, mode and owner’s name are respectively displayed in the fields of the “Current
sample” area.
The “Next Sample” refers to the sample to be analyzed next. Its sample ID is displayed in the
“Next sample” area.
 Analysis Result Area
The Analysis Result area displays the analysis result.
 Error Message Area
The Error Message area displays error messages one by one, alternating every two
seconds.
 Reagent Status Area
The Reagents Status area displays how many counts the remaining reagents are enough for.
Note that when it displays “＞300 counts”, it indicates the reagents are enough for over 300
counts and there is also enough space left in the waste container for the counts; when it
displays “0 counts”, it indicates either at least one of the reagents is insufficient or the waste
container is full.
 Menu Area
When you press [MENU], this area displays the system menu.
 Help Area
The Help area reminds you how to proceed to the next step.
2.4.2 Screen saver

This analyzer will enter the screen saver if it has been idle at the “Count” screen for 10
minutes. When it happens, the sample probe will retract into the analyzer, the LCD will turn
dark and the power indicator will be flickering. You can press any key to resume the display
and reposition the sample probe.
2-11
2.4.3 System Menu

Press the [MENU] button and the system menu, shown in Figure 2-7 below, will pop up.
Figure 2-7 System menu
The system menu contains 8 programs. The programs followed by “ ”s have further
sub-menus. See Figure 2-8 for the expanded menu.
Figure 2-8 Fully expanded system menu
2-12
You can select the desired program as instructed below.
If you want to… Select…
analyze samples Count

select animal type Animal
review sample results Review
run the QC program Quality Control
customize system software Setup
maintain/service the analyzer Service
calibrate the analyzer Calibration
shut down the analyzer Shutdown
2-13
2.5 Reagents, Controls and Calibrators
Because the analyzer, reagents (diluent, rinse, lyse, probe cleanser and E-Z cleanser),
controls, and calibrators are components of a system, performance of the system depends on
the combined integrity of all components. You should only use the Mindray-specified reagents
(see Appendix B Specifications), which are formulated specifically for the fluidic system of
your analyzer in order to provide optimal system performance. If other reagents are used, the
analyzer may not meet the performance specified in this manual and may provide unreliable
results. All references related to reagents in this manual refer to the reagents specifically
formulated for this analyzer.
Each reagent package must be examined before use. Inspect the package for signs of
leakage or moisture. Product integrity may be compromised in packages that have been
damaged. If there is evidence of leakage or improper handling, do not use the reagent.
 Store and use the reagents as directed by instructions for use of the
reagents.
 When you have changed the diluent, rinse or lyse, run a background to see
if the results meet the requirement.
 Pay attention to the expiration dates and open-container stability days of all
the reagents. Never use expired reagents.
 After installing new reagents, let the reagents stand for a while before using
them.
2.5.1 Diluent
The diluent is formulated to meet the following requirements:
 To dilute the blood samples;
 To provide the blood cells with an environment similar to the blood plasma;
 To maintain the cell volume of each red blood cell and platelet during the count
and sizing portion of the measurement cycle;
 To provide a conductive medium for impedance counting of white and red blood
cells and platelets.
2-14
2.5.2 Lyse
The lyse is formulated to meet the following requirements:
 To rapidly break down red blood cell walls, release the hemoglobin from the cell,
and reduce the size of cellular debris to a level that does not interfere with white
blood cell counting.
 To convert hemoglobin to a complex whose absorbance is determined by the

hemoglobin concentration.
2.5.3 Rinse
The rinse is formulated to rinse the bath and metering tubes and to provide proper meniscus
formation in the metering tubes and maintain it during each measurement cycle.
2.5.4 E-Z Cleanser

The E-Z (enzymatic) cleaner is an enzyme-based isotonic, cleaning solution and wetting
agent formulated to clean the fluidic lines and bath.
2.5.5 Probe Cleanser

The probe cleanser is an alkaline cleaning solution formulated to clean the fluidic lines,
aperture and bath.
2.5.6 Controls and Calibrators

The controls and calibrators are used to verify accurate operation of and calibrate the
analyzer.
The controls are commercially prepared whole-blood products used to verify that the analyzer
is functioning properly. They are available in low, normal, and high levels. Daily use of all
levels verifies the operation of the analyzer and ensures reliable results are obtained. The
calibrators are commercially prepared whole-blood products used to calibrate the analyzer.
Read and follow the instructions for use to use the controls and calibrators. All references
related to controls and calibrators in this manual refer to the controls and calibrators reagents
specifically formulated for this analyzer. Controls and calibrators can be purchased from
Mindray or Mindray-authorized distributors.
2-15
3 Understanding the System
Principles
3.1 Introduction
The two independent measurement methods used in this analyzer are:
 the impedance method for determining the WBC, RBC, and PLT data;
 the colorimetric method for determining the HGB.
During each analysis cycle, the sample is aspirated, diluted and mixed before the
determination for each parameter is performed.
3-1
Understanding the System Principles
3.2 Aspiration
This analyzer can process two types of blood samples – whole blood samples and prediluted
blood samples.
If you are going to analyze a whole blood sample, you can simply present the sample to the
sample probe and press the aspirate key to aspirate 13μL of the sample into the analyzer.
If you are going to analyze a capillary blood sample, you should first manually dilute the
sample (20 μL of capillary sample needs to be diluted by 1.6 mL of diluent) and then present
the pre-diluted sample to the sample probe and press the aspirate key to aspirate 0.7 mL of
the sample into the analyzer.
3-2
3.3 Dilution
Usually in blood samples, the cells are too close to each other to be identified or counted. For
this reason, the diluent is used to separate the cells so that they are drawn through the
aperture one at a time as well as to create a conductive environment for cell counting.
Moreover, red blood cells usually outnumber white blood cells by 1,000 times. For this reason,
lyse needs to be added to the sample to eliminate the red cells before the WBC counting.
In addition, to ensure the accuracy of the counting and leukocyte differential, the analyzer
sets different RBC dilution ratios and the amount of lyse for different animal type.
3-3
3.4 WBC/HGB Measurement
3.4.1 Volumetric Metering

An accurate cell count cannot be obtained unless the precise volume of diluted sample that
passes through the aperture during the count portion of the analysis cycle (the count cycle) is
known. This analyzer uses a volumetric metering unit to control the count cycle and to ensure
that a precise volume of sample is analyzed.
The metering unit controlling the WBC count cycle consists of a metering tube with two
optical sensors mounted on it. This tube ensures that a precise amount of diluted sample is
measured during each count cycle. The exact amount is determined by the distance between
the two optical sensors. The rinse is used to create a meniscus in the metering tube. The
count cycle starts when the meniscus reaches the upper sensor and stops when the
meniscus reaches the lower sensor. The amount of time required for the meniscus to travel
from the upper sensor to the lower sensor is called the WBC Count Time and is measured in
seconds. At the end of the count cycle, the measured count time is compared to the
pre-defined reference count time (see Chapter 5.3 for details). If the former is less than or
greater than the latter by 2 seconds or more, the analyzer will report a WBC bubble or WBC
Clog error. Seeing the error message, you can refer to Chapter 11 Troubleshooting Your
Analyzer for solutions.
Figure 3-1 Volumetric metering process
3-4
3.4.2 Measurement Principles

 WBC measurement
WBCs are counted and sized by the impedance method. This method is based on the
measurement of changes in electrical resistance produced by a particle, which in this case is
a blood cell, suspended in a conductive diluent as it passes through an aperture of known
dimensions. An electrode is submerged in the liquid on both sides of the aperture to create an
electrical pathway. As each particle passes through the aperture, a transitory change in the
resistance between the electrodes is produced. This change produces a measurable
electrical pulse. The number of pulses generated indicates the number of particles that
passed through the aperture. The amplitude of each pulse is proportional to the volume of
each particle. Each pulse is amplified and compared to the internal reference voltage
channels, which only accepts the pulses of a certain amplitude. If the pulse generated is
above the WBC threshold, it is counted as a WBC.
Figure 3-2 Impedance method of counting and sizing
 HGB measurement
HGB is determined by the colorimetric method. The WBC/HGB dilution is delivered to the
WBC bath where it is bubble mixed with a certain amount of lyse, which converts hemoglobin
to a hemoglobin complex that is measurable at 525 nm. An LED is mounted on one side of
the bath and emits a beam of monochromatic light, whose central wavelength is 525nm, and
then is measured by a photo-sensor that is mounted on the opposite side. The signal is then
amplified and the voltage is measured and compared to the blank reference reading
(readings taken when there is only diluent in the bath). The HGB is calculated per the
following equation and expressed in g/L.
3-5
HGB(g/L) = Constant×Log 10 (Blank Photocurrent/Sample Photocurrent)
3.4.3 Derivation of WBC-Related Parameters

 WBC
WBC (109/ L) is the number of leukocytes measured directly by counting the white blood cells
passing through the aperture.
WBC  n  109 / L
Note that NRBCs do not react with the lyse and can be mistaken by the analyzer for white
blood cells. If you observe NRBCs in the microscope, correct the system-generated result by
the following formula,
100
WBC'＝WBC 
100＋NRBC
where WBC represents the system-generated white cell number, NRBC the number of
NRBCs counted in 100 white cells and WBC′ the corrected white cell number.
 WBC differential
With the help of the diluent and lyse, this analyzer can size the white cells into three
sub-populations - lymphocytes, monocytes and granulocytes. Based on the WBC histogram,
this analyzer calculates Lymph％, Mon％ and Gran％ as follows and express the results in
percents.
PL
Lymph%   100
PL  PM  PG
PM
Mon%   100
PL  PM  PG
PG
Gran%   100
PL  PM  PG
9
where PL = particles in the lymphocyte region( 10 / L )
9
PM = particles in the monocyte region( 10 / L )
9
PG = particles in the granulocyte region( 10 / L ).
Having achieved the three parameters above, this analyzer proceeds to calculate the
9
Lymph# , Mon# and Gran# per the following equations and express them in 10 / L .
3-6
Lymph%  WBC
Lymph# 
100
Mon%  WBC
Mon# 
100
Gran %  WBC
Gran # 
100
 Eosinophils
Eosinophils (Eos) are sub-population of granulocytes. Granulocytes (Gran) include

neutrophils, eosinophils and basophils.
Eosinophil percecntage (Eos%) is obtained through the distribution of WBC histogram.
 WBC histogram
Besides the parameters mentioned above, this analyzer also presents a WBC histogram,
whose x-coordinate represents the cell volume（fL）and y-coordinate represents the number
of the cells. The histogram is presented in the Analysis Result area of the “Count” screen
when the analysis is done. You can also review the histograms of the stored patient results
(see Chapter 7 Reviewing Sample Results).
The first three discriminators of the WBC histogram can be adjusted in case you are not
satisfied with the result. Note that you cannot adjust them if the WBC result is less than 0.5 or
out of the operating range.
3.4.4 HGB
Using the colorimetric method, this analyzer calculates hemoglobin concentration (g/L) as
follows.
HGB(g/L)=Constant×Log 10 (Blank Photocurrent/Sample Photocurrent)
3-7
3.5 RBC/PLT Measurement
3.5.1 Volumetric Metering

An accurate cell count cannot be obtained unless the precise volume of diluted sample that
passes through the aperture during the count cycle is known. This analyzer uses a volumetric
metering unit to control the count cycle and to ensure that a precise volume of sample is
analyzed for the measurement.
The metering unit controlling the RBC/PLT count cycle consists of a metering tube with two
optical sensors mounted on it. This tube ensures that a precise amount of diluted sample is
measured during each count cycle. The exact amount is determined by the distance between
the two optical sensors. The rinse is used to create a meniscus in the metering tube. The
count cycle starts when the meniscus reaches the upper sensor and stops when the
meniscus reaches the lower sensor. The amount of time required for the meniscus to travel
from the upper sensor to the lower sensor is called the RBC Count Time and is measured in
seconds. At the end of the count cycle, the measured count time is compared to the
pre-defined reference count time (see Chapter 5.3 for details). If the former is less than or
greater than the latter by 2 seconds or more, the analyzer will report an RBC bubble or RBC
clog error. Seeing the error message, refer to Chapter 11 Troubleshooting Your Analyzer
for solutions.
Figure 3-3 Volumetric metering process
3-8
3.5.2 Measurement Principles

 RBC/PLT measurement
RBCs/PLTs are counted and sized by the impedance method. This method is based on the
measurement of changes in electrical resistance produced by a particle, which in this case is
a blood cell, suspended in a conductive diluent as it passes through an aperture of known
dimensions. An electrode is submerged in the liquid on both sides of the aperture to create an
electrical pathway. As each particle passes through the aperture, a transitory change in the
resistance between the electrodes is produced. This change produces a measurable
electrical pulse. The number of pulses generated indicates the number of particles that
passed through the aperture. The amplitude of each pulse is proportional to the volume of
each particle. Each pulse is amplified and compared to the internal reference voltage
channels, which only accepts the pulses of a certain amplitude. If the pulse generated is
above the RBC/PLT lower threshold, it is counted as an RBC/PLT.
Figure 3-4 Impedance method of counting and sizing
3.5.3 Derivation of RBC-Related Parameters
 RBC
RBC (1012/L) is the number of erythrocytes measured directly by counting the erythrocytes
passing through the aperture.
 MCV
Based on the RBC histogram, this analyzer calculates the mean cell volume (MCV) and
3-9
expresses the result in fL.

This analyzer calculates the HCT (%), MCH (pg) and MCHC (g/L) as follows:
RBC  MCV
HCT 
10
HGB
MCH 
RBC
HGB
MCHC   100
HCT
Where the RBC is expressed in 1012/L, MCV in fL and HGB in g/L.
 RDW
Based on the RBC histogram, this analyzer calculates the CV (Coefficient of Variation) of the
erythrocyte distribution width.
 RBC Histogram
Besides the parameters mentioned above, this analyzer also presents an RBC histogram,
3.5.4 Derivation of PLT-Related Parameters
 PLT
PLT (109/L) is measured directly by counting the platelets passing through the aperture.
 MPV
Based on the PLT histogram, this analyzer calculates the mean platelet volume (MPV, fL).
 PDW
Platelet distribution width (PDW) is the geometric standard deviation (GSD) of the platelet
size distribution. Each PDW result is derived from the platelet histogram data and is reported
as 10 (GSD).
 PCT
This analyzer calculates the PCT as follows and express it in ％.

Where the PLT is expressed in 109/L and the MPV in fL.
3-10
PLT  MPV
PCT 
10000
 PLT Histogram
Besides the parameters mentioned above, this analyzer also presents a PLT histogram,
3-11
3.6 Wash
After each analysis cycle, each element of the analyzer is washed.
 The sample probe is washed internally and externally with diluent;
 The bath is washed with diluent and rinse;
 The metering tube is washed with rinse;
 The rest of the fluidic system is washed by diluent.
3-12
4 Installing Your Analyzer
4.1 Introduction
This chapter introduces how to install the BC-2800Vet. To ensure all system components
function correctly and to verify system performance, Mindray-authorized representatives will
handle the installation and initial software setup.
 Installation by personnel not authorized or trained by Mindray may damage

your analyzer. Do not install your analyzer without the presence of
Mindray-authorized personnel.
4-1
Installing Your Analyzer
4.2 Installation Requirements
Before installation, you should ensure that the following space, power and environmental
requirements are met.
4.2.1 Space Requirements

Check the site for proper space allocation. In addition to the space required for the analyzer
itself, arrange for
 at least 28 cm on each side, which is the preferred access to perform service

procedures;
 at least 10 cm behind for cabling and ventilation;
 enough room on or below the countertop to accommodate the diluent, rinse and
waste containers.
4.2.2 Power Requirements

Check the availability of a power outlet that meets the following requirements
 Voltage: 100 to 240 VAC
 Frequency: 50/60±1 Hz
 Power: 180 VA
 Fuse: 250V T4A
 Make sure the analyzer is properly grounded.
 Replace fuse only with the type and rating specified.
 Make sure the electrical outlet meets the requirements.
 Before connecting the power cord, make sure the power switch at the back
of the analyzer is placed in the off (O) position.
4-2
4.2.3 General Environment

 Operating temperature: 15 ℃ to 30 ℃.
 Relative humidity: 30% to 85%.
 Atmospheric pressure: 60 kPa to 106 kPa.
 The environment should be as free as possible from dust, mechanical vibrations,

loud noises, and electrical interference.
 Do not place the analyzer near brush-type motors, flickering fluorescent lights, and
electrical contacts that regularly open and close.
 Do not place the analyzer in direct sunlight or in front of a source of heat or drafts.
 It is advisable to evaluate the electromagnetic environment prior to operation of

this analyzer. Do not use the device in close proximity to sources of strong
electromagnetic radiation (e.g. unshielding intentional RF sources).
 The environment should be good ventilation.
 Do not place the analyzer in a flammable or explosive environment.
 Do not place any container on the top of the analyzer.
 If the ambient temperature is outside the specified operating range, the

analyzer will alarm you for abnormal ambient temperature and the analysis
results may be unreliable. See Chapter 11 Troubleshooting Your Analyzer for
solutions.
4-3
4.3 Unpacking
4.3.1 Unpacking and Inspecting the Analyzer

Your analyzer is tested before it is shipped from the factory. International symbols and special
handling instructions tell the carrier how to treat this electronic instrument. When you receive
your analyzer, carefully inspect the carton. If you see any signs of mishandling or damage,
contact Mindray customer service department or your local distributor immediately. When you
are sure the carton is fine, follow the steps below to unpack the analyzer:
 Place the carton on the floor upright with the arrows on the side upwards;
 Remove the tape and take out the accessory box. Check the accessories against
the packing list. Notify the Mindray customer service department or your local
distributor immediately if you find anything missing;
 Open the main box and check the items inside against the packing list. Notify the
Mindray customer service department or your local distributor immediately if you
find anything missing;
 Remove the top protective foam, carefully carry out the analyzer from the box and
place it on the countertop.
 Remove the foam fixing the sample probe.
 Retain the shipping carton and all the packing materials, as they can be
used for packaging if analyzer must be reshipped.
4.3.2 How to move the analyzer

 If the carton is intact, you may use a plate and fork-lifter to move the analyzer for a
short distance.
 If your analyzer has been used, do the ”Empty tubing” procedure and shut it
down before moving it.
 For a short - distance moving on a smooth ground, you may use a trolley to
facilitate the transportation.
 During the moving process, protect the LCD and the sample probe from excessive
force and from contact with other objects.
 Keep the analyzer upright during the moving process. Do not tilt or incline it.
 Do your best to minimize the mechanical shock when moving the analyzer. After a
long-distance moving, check and tune the analyzer before using it.
4-4
4.4 Installation Procedure
 Dispose of reagents, waste, samples, consumables, etc. according to

government regulations.
 The reagents are irritating to eyes, skin and diaphragm. Wear proper
personal protective equipment (e.g. gloves, lab coat, etc.) and follow safe
laboratory procedures when handling them in the laboratory.
 If the reagents accidentally spill on your skin, wash them off with plenty of
water and if necessary, go see a doctor; if the reagents accidentally spill into
your eyes, wash them off with plenty of water and immediately go see a
doctor.
 Use the specified reagents.
 After installing new reagents, let them stand for a while before using them.
 Never use expired reagents.
 To prevent contamination, tighten the container caps when the installation is

finished.
 Samples, controls, calibrators and waste are potentially infectious. Wear

proper personal protective equipment (e.g. gloves, lab coat, etc.) and follow
safe laboratory procedures when handling them in the laboratory.
4.4.1 Connecting Regents

On the back of the instrument you will find a fluidic connection equipped with 4 plastic plugs.
These plugs are closed by protection caps to prevent dust and the leaking of liquids during
transportation. Take off these caps by unscrewing them and keep them in a safe place for
future transportation.
Connecting the diluent container

1. Take out the diluent pickup tube (the one with a green connector) from the accessory
box.
4-5
2. Remove the container cap and insert the tube end that has no connector into the diluent
container and tighten the cap until properly secured, as Figure 4-1 shows;
Diluent
Green tubing connector
Figure 4-1 Connecting the diluent container
3. Locate the green fitting marked “DILUENT” in the lower right corner of the back of the
analyzer;
4. Plug the green connector of the tube into the fitting and turn it clockwise until properly
secured.
Connecting the rinse container

1. Take out the rinse pickup tube (the one with a blue connector) from the accessory box.
2. Remove the container cap and insert the tube end that has no connector into the rinse
Rinse
Blue tubing connector
Figure 4-2 Connecting the rinse container
4-6
3. Locate the blue fitting marked “RINSE” in the lower right corner of the back of the
analyzer.
4. Plug the blue connector of the tube into the fitting and turn it clockwise until properly
secured.
Connecting the lyse container

1. Take out the lyse pickup tube (the one with an orange connector) from the accessory box.
2. Remove the container cap and insert the tube end that has no connector into the
Lyse
Orange tubing connector
Figure 4-3 Connecting the lyse container
3. Locate the orange fitting marked “LYSE” in the lower right corner of the back of the
analyzer;
4. Plug the orange connector of the tube into the fitting and turn it clockwise until properly
secured.
Connect the waste container

1. Take out the waste tube (the one with a red connector) from the accessory box;
2. Locate the red fitting marked “WASTE” in the lower right corner of the back of the
analyzer;
3. Plug the red connector of the tube into the fitting and turn it clockwise until properly
secured;
4. Prepare a container to receive the waste and place it on or below the countertop;
5. Insert the waste tube into the waste container.
4.4.2 Installing Recorder Paper
4-7
 Remove the protective paper between the recorder head and the roller
inside the recorder before installing recorder paper.
Follow the procedure below to install the recorder paper.
1. Use the latch at the upper right corner of the recorder door to pull the door open.
2. Insert a new roll into the compartment as shown below.
3. Close the recorder door.
4. Check if paper is installed correctly and the paper end is feeding from the top.
Paper roll
Figure 4-1 Installing recorder paper
 Use only specified recorder paper. Otherwise, it may cause damage to the
recorder head, or the recorder may be unable to print, or poor print quality
may result.
 Never pull the recorder paper with force when a recording is in process.
Otherwise, it may cause damage to the recorder.
 Do not leave the recorder door open unless you install paper or remove
trouble.
 Improper installation of recorder paper may jam the paper and/or result in
blank printout.
4.4.3 Connecting the Keyboard

Take out the keyboard from the accessory kit and connect it to the keyboard interface marked
“KB”.
4-8
4.4.4 Connecting the Printer (Optional)

Follow the printer’s instructions for use to connect the printer to the parallel port.
4.4.5 Connecting the Bar-Code Scanner (Optional)

Follow the scanner’s instructions for use to connect the scanner to the serial port1.
4-9
4.5 Starting the Analyzer
Take out the power cord from the accessory box. Plug the non-pronged end into the AC input
at the back of the analyzer and the pronged end into an electrical outlet. Place the power
switch at the back of the analyzer in the ON position (1) to turn on the analyzer. The power
indicator light will be illuminated and the screen will display “Initializing…“. The analyzer will
sequentially initialize the file, hardware and fluidic systems and the whole initializing process
lasts about 4 to 7 minutes. When the initialization is finished, the analyzer will automatically
enter the “Count” screen.
4-10
5 Customizing the Analyzer Software
5.1 Introduction
The BC-2800Vet is a flexible laboratory instrument that can be tailored to your work
environment. You can use the “Setup” program to customize the software options as
introduced in Chapters 5.2 to 5.3.
5-1
Customizing the Analyzer Software
5.2 Password
The BC-2800Vet classifies users into two categories: common users (default) and
administrators. You need to enter the administrator password to adjust certain options such
as “Count”, “Gain”, etc.
5.2.1 Entering the administrator password

Press [MENU] to enter the system menu.
SELECT “Setup → Password” ( Figure 5-1 ) to enter the ”Password” screen ( Figure 5-2 ).
Figure 5-2 “Password” screen
ENTER “2826” and press [MENU], a message box will pop up to remind you of the current
password level, as Figure 5-3 shows, to remind you of the current password level,
5-2
Figure 5-3 A message box to confirm the user level
CLICK “Yes” to confirm the password and exit to the system menu.
5.2.2 Resuming the common user password

Enter the “Password” screen and the default password is the common user password. Press
[MENU] again and a message box will pop up to remind you of the current password level, as
Figure 5-4 shows.
Figure 5-4 A message box to confirm the user level
CLICK “Yes” to confirm the password and exit to the system menu.
5-3
5.3 Editing Settings
You can use the “Settings” menu to edit system settings.
Press [MENU] to enter the system menu. SELECT “Setup → Settings“, as Figure 5-5 shows,
to enter the “Settings” screen, as Figure 5-6 shows.
Figure 5-6 “Settings” screen
This screen can be interpreted as follows:
 Setting Groups area (on the left)
This area displays the visible or changeable setting groups. You can press [F1] to select the
desired group. The selected group is preceded by a ⊙.
 Settings area (on the right)
You can change the settings of the items displayed in this area.
 Help area (on the bottom)
5-4
This area displays useful information to help you move to the next step.
At this screen, if you want to acquire help information, press [HELP]; if you want to return to
the system menu, press [MENU].
5.3.1 Reagent
You can select the “Reagent” group to change the settings regarding the reagents and the
waste, as Figure 5-7 shows.
Figure 5-7 “Reagent” settings
 Selecting the “Reagent” group
Press [F1] to select the “Reagent” group.
 Setting remaining volumes for reagents
You may set the remaining volumes for the diluent, rinse and lyse. When any of the entered
volumes is counted down to zero, the system will remind you to install a new container.
1. SELECT “Diluent”, “Rinse” or “Lyse” in the “Remains” field as needed;
2. ENTER the desired digits. See Table 5-1 for the valid reagent volumes.
Table 5-1 Valid reagent volumes
Diluent Rinse Lyse

Allowed range Approx 0 to 30.0 L Approx 0 to 30.0 L Approx 0 to 999.0 mL
 Entering the usable volume of the waste container
You may enter the usable volume of the waste container. When the system counts down the
entered volume to 0, it will alert you to empty the waste container. Follow the steps given
below to set the volume.
5-5
1. SELECT “Waste Container”;
2. ENTER the desired digits.
 Entering expiration dates of reagents
You can specify the expiration dates for the diluent, rinse and lyse. Once any of these
reagents is expired, the system will alert you to install a new container. Follow the steps given
below to enter the expiration dates.
1. SELECT “Exp. Date”;
2. ENTER the desired digits;
3. Note that open reagents are stable for 60 days. The entered expiration date should be
the open date + 60 days or the expiration date marked on the packaging of the reagent,
whichever is earlier.
 Exiting the “Reagent” group
When you have finished changing all the reagent settings, you may
1. Press [F1] to select another setting group you want to change; or
2. Press [MENU] and a message box will pop up to remind you to save the changes, as
Figure 5-8 shows. CLICK “Yes” to save the changes and exit to the system menu; or
CLICK “No” to exit to the system menu without saving the changes.
Figure 5-8 Saving changes
Note that if any entered value is beyond the valid range, a message box will pop up after you
have pressed [MENU]. CLICK “Yes” to close the message box and clear the invalid values.
5-6
Figure 5-9 A message box to remind you of the erroneous entry
5.3.2 Printing and Communication Settings

You can select the “Print & comm.” group to change the printing and communication settings
to your own need, as Figure 5-10 shows.
Figure 5-10 Printing and communication settings
 Selecting the “Print & comm.” group
Press [F1] to select the “Print & comm.” group.
 Selecting the printing device
To select a printing device, SELECT “Recorder” or “Printer” from the “Device” pull-down
list, as Figure 5-10 shows.
 Selecting the printing format
To select a printing format, SELECT “Vertical” or “Horizontal” from the “Format” pull-down
list,
 For the external printer, you can only select “Vertical”.
5-7
 Printout with histogram
SELECT “With histogram”, if you want to include histograms in the printout, as Figure 5-10
shows. To deselect this option, SELECT it again.
 Printout with WBC differential results
The WBC differential count is applicable to cats, dogs, horses, rats, mice, rabbits, monkeys
and cows only. You can choose whether to include the differential results in the printout. To
include the differential results, SELECT “With WBC diff. result”. To deselect this option,
SELECT it again.
 Printout with PLT-related results
You can choose whether to include the PLT-related results in the printout. To include the
PLT-related results, SELECT “With PLT-related result”. To deselect this option, SELECT it
again.
 Auto printing
The auto printing function refers to the analyzer’s ability to automatically print out the analysis
results once they are done. To activate this function, SELECT “AutoPrint”. To deactivate the
function, SELECT it again.
 Setting baud rate
To choose one of the five baud rates”19200”, “9600”, “4800”, “2400” and “1200”, SELECT the
desired baud rate from the “Baud” pull-down list, as Figure 5-11 shows.
Figure 5-11 Selecting baud rate
5-8
 Selecting parity
To choose the “Odd”, “Even” or “None” (default) check, SELECT the desired check from the
“Parity” pull-down list, as Figure 5-12 shows.
Figure 5-12 Setting parity
 Activating/deactivating handshake
If the “Handshake” function is activated, to start the transmission this analyzer will send a
handshake signal to an external computer and wait for the response. If the computer does not
respond, this analyzer will abort the transmission and give an alarm for the transmission error.
If the “Handshake” function is deactivated, this analyzer will transmit data to the external
computer regardless of the response. This function is deactivated by default.
To activate or deactivate this option, SELECT “Yes” or ”No” from the “Handshake”
pull-down list, as Figure 5-13 shows.
Figure 5-13 Setting handshake
5-9
 Auto Communication
The auto communication function refers to the analyzer’s ability to automatically transmit the
analysis results to an external computer once they are done. To activate or deactivate this
function, SELECT “On” or ”Off” from the “AutoComm” pull-down list in the “Transmit” field,
as Figure 5-14 shows.
Figure 5-14 Auto communication
 Editing report titles (external keyboard needed)
To edit the title of an analysis report,
1. SELECT “Recorder” or ”Printer” in the “Report Title” field, depending on the selected
printing device;
2. ENTER the desired report title.
 Exiting the “Print & comm.” group
When you have finished changing all the printing and transmission settings, you may press
[F1] to select another setting group you want to change; or press [MENU] and a message box
will pop up to remind you to save the changes, as Figure 5-15 shows. CLICK “Yes” to save
the changes and exit to the system menu; or CLICK “No” to exit to the system menu without
saving the changes.
5-10
5.3.3 Date and Time

You can select the “Date & time” group to set the system date and time, as Figure 5-16
shows. Follow the instructions given below to do so.
Figure 5-16 Setting date and time
 Selecting the “Date & time” group

Press [F1] to select the “Date & time” group.
 Setting the system date
1. SELECT “Year”, ” Month” or ”Day”;
5-11
 Selecting the date format
You may choose one of the three formats “YYYY-MM-DD”, “MM-DD-YYYY“ and
“DD-MM-YYYY”. To do so, SELECT the desired format from the “Format” pull-down list, as
Figure 5-17 shows.
Figure 5-17 Setting date format
 Setting the system time
1. SELECT “Hour”, “Minute” or “Second”;
 Exiting the “Date & time” group
When you have finished changing all the date and time settings, you may
1. Press [F1] to select another setting group you want to change; or;
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5.3.4 Gain
You can select the “Gain” group to view or change (if you have the administrator password)
the WBC, RBC and HGB gains.
Figure 5-19 Setting gain
 Selecting the “Gain” group
Press [F1] to select the “Gain” group, as Figure 5-19 shows.
 Setting the RBC gain
When the difference between the actual MCV result and the expected result exceeds 6%,
5-13
you need to change the RBC gain.
For example, assuming the expected MCV result is 90.0fL, while the actual analysis result is
82.0fL, then
ExpectedMCV 90.0
 100%＝  100%  109.8%
ActualMCV 82.0
You should adjust the RBC gain to 109.8% as close as possible. Follow the steps given below
to do so.
1. Enter the administrator password as introduced in Chapter 5.2.1;
2. At the “Settings” screen, press [F1] to select the “Gain” group;
3. SELECT “RBC”, as Figure 5-20 shows;
4. ENTER the desired gain so that the adjustment becomes as close to 109.8% as possible.
Figure 5-20 Setting RBC gain
 Setting the HGB gain
You may adjust the HGB gain to change the HGB blank voltage, which usually should be set
between 3.4 to 4.8V (4.5V recommended). Follow the steps given below to set the HGB gain.
2. At the “Settings” screen, press [F1] to select the “Gain” group;
3. SELECT “HGB”;
4. ENTER the desired gain so that the HGB blank voltage is between 3.4 to 4.8V, as Figure
5-21 shows.
5-14
Figure 5-21 Setting HGB gain
 Exiting “Gain” group
When you have finished changing all the gain settings, you may
5-15
5.3.5 Count
You can select the “Count” group to view or change (if you have the administrator password)
the parameter units and count time.
Figure 5-23 Setting unit and count time
 Selecting the “Count” group
Press [F1] to select the “Count” group, as Figure 5-23 shows.
 Selecting parameter units
This analyzer provides multiple units for certain parameters. Refer to Table 5-2 for all the
selectable units of all parameters. The 19 parameters are divided into 11 groups based on
their units and you can only select unit for the first parameter of a group. Pay special attention
to the HGB group, which includes HGB, MCHC and MCH. When you select g/L or g/dL as the
unit of HGB, the default unit for MCH is pg; when you select mmol/L as the unit of HGB, the
default unit of MCH is fmol.
Table 5-2 Units of Parameters
Parameter Display format Unit Remarks

WBC ***.* 109/L Default
Lymph# ***.* 103/uL /
Mon# **** 102/uL /
Gran# ***.* /nL /
Lymph% **.* Default
5-16
Mon% .*** %
Gran%
Eos%
HGB, MCHC *** g/L Default
**.* g/dL /
**.* mmol/L /
12
RBC **.** 10 /L Default
6
**.** 10 /uL /
4
**** 10 /uL /
**.** /pL /
HCT **.* % Default

.*** L/L /
MCV ***.* fL Default
3
***.* um /
RDW **.* % Default
9
PLT **** 10 /L Default
3
**** 10 /uL /
4
***.* 10 /uL /
**** /nL /
MPV ***.* fL Default

3
***.* um /
PDW **.* / Default
PCT .*** % Default
*.** mL/L /
MCH *.*** pg Default
**.** fmol /
Follow the steps given below to select the units.
2. At the “Settings” screen, press [F1] to select the “Count” group;
3. SELECT the desired unit from the pull-down list of the desired parameter, as Figure
5-24 shows.
5-17
Figure 5-24 Selecting parameter unit
 Setting count time
If the WBC or RBC count time is inappropriately set, the system may give false alarms for
clogs or bubbles. When this happens, follow the steps given below to change the WBC or
RBC count time. Refer to the actual count time (see Chapter 10.4.1 for details) when editing
the count time. Follow the steps given below to set the count time.
2. At the “Settings” screen, press [F1] to select the “Count” group;
3. SELECT “WBC” or “RBC”, as Figure 5-25 shows;
5-18
Figure 5-25 Setting count time
 Exiting the “Count” group
When you have finished changing all the parameter units and count time settings you want to
change, you may
5.3.6 Reference Range

If you have the administrator password, you can set a reference range for every parameter of
the currently selected animal (see Chapter 6.5 for how to select the animal). The system will
flag any parameter result that exceeds this range with either an H (higher than the upper limit)
or L (lower than the lower limit). The default ranges are given. Note that the default ranges
are for references only. Each laboratory is encouraged to establish its own references.
As for the user-defined animals, reference ranges are available for 12 parameters, excluding
the six parameters related to the WBC differential count.
 Setting reference ranges (administrators only)

2. At the “Settings” screen, press [F1] to select the “Ref. Range” group, as Figure 5-27
shows;
5-19
Figure 5-27 Setting reference range
3. SELECT the desired parameter and ENTER the desired digits for the upper and lower
limits;
4. Press [F2] to save the changes. If the changes are successfully saved, a message box
shown in Figure 5-28 will pop up; CLICK the “Yes” to close the message box;
Figure 5-28 A message box to note data saved
5. If some entered limits are invalid, a message box shown in Figure 5-29 will pop up.
CLICK “Yes” and reenter valid number;
Figure 5-29 A message box to note an erroneous entry
6. If you want to resume the default settings, press [F4].
 Exiting the “Ref. Range” group
When you have finished changing all the reference range settings you want to change, you
may
2. Press [MENU] to exit to the system menu.
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5.3.7 Other Settings

You can select the “Other” group to view or change other settings.
 Selecting the “Other” group
Press [F1] to select the “Other” group.
 Muting beeper
This analyzer beeps when an error occurs. You can mute the beeper by pressing any key or
leave it beeping until the errors are removed. If you prefer the former, SELECT “Enabled”
from the “Any key to mute” pull-down list ; if you prefer the latter, SELECT “Disabled”
from the “Any key to mute” pull-down list. See Figure 5-30 shows.
Figure 5-30 Selecting how to mute the beeper
 Selecting LCD contrast
Follow the steps given below to adjust the LCD contrast.
1. SELECT “LCD contrast”, as Figure 5-31 shows;
2. ENTER the desired contrast (0 to 255).
5-21
Figure 5-31 Select LCD contrast
 Setting alarm time
Follow the steps given below to set for how long (2s to120s) the error messages listed in
Table 5-3 should be displayed on the screen.
Table 5-3 Error List
No. Error No. Error No. Error

1 Communication Error 2 Scanner Error 3 Scanner
Communication Error
4 Environmental 5 Background Abnormal 6 HGB Error
Temperature Abnormal
7 HGB Adjustment 8 WBC Clog 9 WBC Bubbles
10 RBC Clog 11 RBC Bubbles
1. SELECT “Alarm time(s)”, as Figure 5-32 shows;
5-22
Figure 5-32 Setting alarm time
 Selecting PMB color (administrator password needed)
The PMB color refers to the background color of the screen when your analyzer is in the
prediluted mode. Follow the steps below to select the PMB color.
2. At the “Settings” screen, press [F1] to select the “Other” group;
3. SELECT “Black” (default) or “Blue” from the “PMB color” pull-down list, as Figure 5-33
shows.
Figure 5-33 Setting PMB color
 Exiting the “Other” group
5-23
When you have finished changing all the ”Other” settings you want to change, you may
Figure 5-34 shows. SELECT “Yes” to save the changes and exit to the system menu; or
SELECT “No” to exit to the system menu without saving the changes.
5.3.8 Special Fluidic Settings (for user-defined animals)

For the user-defined animals, the “Fluidic settings” group will be enabled. You can select the
“Fluidic settings” group to view or change (if you have the administrator password) the listed
fluidic settings of the currently selected animal. Note that the default settings are for your
reference only. You need to find the settings that fit the animal the best.
Figure 5-35 “Fluidic settings” screen
5-24
 Adjusting the fluidic settings
2. At the “Settings” screen, press [F1] to select the “Fluidic settings” group;
3. SELECT the desired settings;
4. ENTER the desired digits for ”Lyse used (wb)” or “Lyse used (pre)” (Figure 5-35),
 Exiting the “Fluidic settings” group
When you have finished changing all the parameter units and count time settings you want to
change, you may
5-25
6 Operating Your Analyzer
6.1 Introduction
This chapter provides step-by-step procedures for operating your analyzer on a daily basis.
6-1
Operating Your Analyzer
6.2 Initial Checks
Perform the following checks before turning on the analyzer.
1. Check and make sure the waste container is empty;
2. Check and make sure there are enough reagents;


3. Checking tubing and power connections;
 Check and make sure the diluent, rinse and waste tubes are properly connected
and not bent;
 Check and make sure the power cord of the analyzer is properly plugged into an
electrical outlet.
4. Checking the printer (optional) and recorder;
Check and make sure enough printer or recorder paper is installed. Check and make sure the
power cord of the printer is properly plugged into an electrical outlet. Check and make sure
the printer cable is properly connected to the analyzer.
5. Check keyboard connection.
Check and make sure the keyboard is properly connected to the keyboard interface (marked
“KB”) of the analyzer.
6-2
6.3 Power-on
Place the power switch at the back of the analyzer in the ON position (1) to turn on the
analyzer. The power indicator light will be illuminated and the screen will display
“Initializing…“.
The analyzer will sequentially initialize the file, hardware and fluidic systems and the whole
initializing process lasts 3 to 5 minutes, depending on how the analyzer was previously shut
down.
If any error occurs during the initialization, the analyzer will display the error messages in the
lower left corner of the screen. You should remove all the errors before running any sample.
See Chapter 11 Troubleshooting Your Analyzer for solutions.
 Running samples with the abnormal background error present will lead to
misleading results.
6-3
6.4 Daily Quality Control
Before running any samples, run the controls. See Chapter 8 Using the QC Programs for
details.
6-4
6.5 Selecting Animal
1. Press [MENU] to enter the system menu, as Figure 6-1 shows;
2. SELECT “Animal” to enter the “Animal” screen, as Figure 6-2 shows;
Figure 6-2 “Animal” screen
3. SELECT the desired animal;
4. Press [MENU] to exit to the system menu.
 Once you have selected another animal, the analysis result and sample
information regarding the current sample will be cleared, but the next
sample ID will remain unchanged.
6-5
6.6 Define an animal
You can define a maximum of 3 user-defined animals. Follow the steps below to do so:
1. At the “Animal” screen, press the arrow keys as needed to move the cursor to a
user-defined animal (“Animal1” to “Animal3”);
2. Press [F1] to activate the edit box and ENTER the animal name;
3. Press [F1] to deactivate the edit box.
6-6
6.7 Sample Collection and Handling

 Avoid direct contact with blood samples.
 Do not re-use disposable products.
6.7.1 Whole Blood Samples

Collect and handle the whole blood sample as follows:
1. Collect venous blood;
2. Rapidly and thoroughly mix the blood with the anticoagulant, K2EDTA (1.5 to 2.2mg/mL ).
 For the whole blood samples to be used for WBC differential or PLT count,
you shall store them at the room temperature and run them within 4 hours
after collection.
 If you do not need the PLT, MCV and WBC differential results, you can store
the samples in a refrigerator (2℃ to 8℃) for 24 hours. You need to warm the
refrigerated samples at room temperature for at least 30 minutes before
running them.
 Mix any sample that has been prepared for a while before running it.
6.7.2 Prediluted Samples

Collect and handle the prediluted sample as follows:
1. Press [MENU] and SELECT ”Count” to enter the ”Count” screen;
6-7
2. Press [MODE] to select analysis mode “PB”;
3. Press [DILUENT] and a message box will pop up to instruct you how to dispense the
diluent into the sample tube, as Figure 6-3 shows;
Figure 6-3 A message box showing you how to dispense diluent
4. Present a clean sample tube to the sample probe and make sure the tube is tilted
towards the probe, as Figure 6-4 shows, to avoid spills and bubbles. Press the aspirate
key to dispense 0.7mL of diluent (the dispensing volume is controlled by the analyzer)
into the tube;
Figure 6-4 How to dispense diluent
5. When the dispensing is finished, press [ENTER] to close the message box;
6. Add 20μL of capillary blood to the diluent and shake the tube to mix the sample.
6-8
 Keep dust from the prepared diluent.
 After mixing the capillary sample with the diluent, wait 3 minutes before
running the sample.
 Run the prediluted samples within 15 minutes after the mixing.
 Evaluate predilute stability based on your laboratory’s sample population

and sample collection techniques or methods.
6-9
6.8 Running Whole Blood Samples
Press [MENU] and SELECT ”Count” to enter the ”Count” screen, as Figure 6-5 shows.
Figure 6-5 “Count” screen
Press [MODE] to select “WB” mode;
 Select proper reference range as instructed in Chapter 5.3.6 before running

the samples. Otherwise, the obtained results may be erroneously flagged.
6.8.1 Entering Sample Information

At the “Count” screen, you can enter the sample information for the next sample (the sample
to be analyzed) and edit the sample information for the current sample (the sample whose
analysis results are displayed on the screen).
Entering the sample inforamtion of the next sample(external keyboard

needed)
To enter the sample ID of the next sample, you may
At the “Count” screen, use the bar-code scanner (if available) to scan the sample ID into the
analyzer; or
At the “Count” screen, press [F1] to enter the “Enter sample information” window and
ENTER the information of the next sample, as Figure 6-6 shows.
6-10
Figure 6-6 Entering ID of the next sample
 Entering the sample ID
Enter the sample ID into the “ID” box.
 Selecting animal gender
SELECT the desired item from the “Gender” pull-down list, as Figure 6-7 shows. Note that
you can select blank in case you are not aware of the patient gender.
Figure 6-7 Selecting animal gender of the nest sample
 Entering the owner’s name
ENTER the owner’s name into the ”Owner” box.
6-11
 Entering the animal’s name
ENTER the animal’s name into the ”Animal” box.
 Entering the animal’s age
This analyzer provides three ways for you to enter the patient age –in years, in months and in
days.
To enter the animal’s age in years: ENTER the desired number, an integer from 0 to 200, into
the “Years” box.
To enter the patient age in months: ENTER the desired number, an integer from 0 to 12, into
the “Months” box.
To enter the patient age in days: ENTER the desired number, an integer from 0 to 31, into the
“Days” box.
 Exit edit
When you have finished entering all the desired sample information, CLICK the “Yes” button
to save the changes and return to the “Count” screen. If you do not want to save the entered
information, CLICK the “No” button to return to the ”Count” screen without saving the
changes.
Figure 6-8 Dialog box
 If you intend to do the background check instead of a patient sample, enter

“0” into the “ID” box.
 After entering the information into any field of the “Sample information”
6-12
box, you can press [enter] to directly finish entering while saving the
changes and closing the box.
Editing sample information of the current sample (external keyboard

needed)
 Entering Edit
At the “Count” screen, after running an analysis, press [F4] and an edit window will pop up,
Figure 6-9 Entering sample information
SELECT the desired item from the “Gender” pull-down list, as Figure 6-10 shows. Note
that you can select blank in case you are not aware of the patient gender.
Figure 6-10 How to select the patient gender
ENTER the owner’s name into the “Owner” box.
6-13
ENTER the animal’s name into the “Animal” box.
 Entering the animal age
days.
“Days” box.
 Exit edit
changes.
6.8.2 Running the Samples

 The sample probe tip is sharp and may contain biohazardous materials.
Exercise caution to avoid contact with the probe when working around it.
 Do not re-use such disposable product as collection tubes, test tubes,
6-14
capillary tubes, etc.
 Keep the sample probe tip away from the tube bottom; otherwise the
aspiration volume may be inaccurate.
 When the aspiration is done, remove the sample tube only when the sample
probe is out of the tube.
1. At the “Count” screen, be sure the System Status area displays “Ready“ and the Count
Mode area displays “WB“;
2. Present the mixed sample to the sample probe so that the tip is well into the tube, and
press the aspirate key. The System Status area will display “Running” and the analyzer
will start aspirating sample;
3. When you hear the beep and the sample probe is out of the tube, remove the sample
tube. The sample probe will retract into the analyzer and the analysis progress will be
displayed on the screen;
4. When the analysis is finished, the result will be displayed on the screen and the sample
ID will automatically increase by 1 and the sample probe will be repositioned. And if the
auto print function is enabled, the analysis result will be automatically printed out;
5. Repeat the above steps on other samples.
 If the analyzer detects WBC/RBC clogging or bubbles during the analysis,

the corresponding error messages will be displayed in the error message
area and the results of all the related parameters will be invalidated. See
Chapter 11 Troubleshooting Your Analyzer for solutions.

solutions.
6.8.3 Special Functions
6-15
Automatic saving of analysis results

This analyzer automatically saves a maximum of 10,000 sample results. When the maximum
number has been reached, the newest result will overwrite the oldest.
Parameter flags
 If the analysis result is followed by an ”H” or “L”, it means the analysis result has
exceeded the upper or lower limit of the reference range.
 If you see *** as opposed to the result, it means the result is either unreliable or
 If the WBC result is less than 0.5  109/L, this analyzer will not perform the
differential analysis and all the related parameter values will be non-numeric (***).
 The result of the background check will not be flagged.
6-16
6.9 Running Prediluted Samples
Press [MENU] and SELECT ”Count” to enter the ”Count” screen, as Figure 6-11 shows.
Figure 6-11 “Count” screen
Press [MODE] to select “PB” mode;

6.9.1 Entering Sample Information

At the “Count” screen, you can enter the sample information for the next sample (the sample
to be analyzed) and edit the sample information for the current sample (the sample whose
analysis results are displayed on the screen).
Entering the sample information of the next sample(external keyboard

needed)
To enter the sample ID of the next sample, you may
At the “Count” screen, use the bar-code scanner (if available) to scan the sample ID into the
analyzer; or
At the “Count” screen, press [F1] to enter the “Enter sample information” window and
ENTER the information for the next sample, as Figure 6-12 shows.
6-17
Figure 6-12 Entering ID of the next sample
 Entering the sample ID
Enter the sample ID into the “ID” box.
Figure 6-13 Selecting animal gender of the nest sample
6-18
days.
“Days” box.
 Exit edit
changes.
Figure 6-14 Dialog box
6-19

Editing sample information of the current sample (external keyboard

needed)
 Entering Edit
At the “Count” screen, after running an analysis, press [F4] and an edit window will pop up,
Figure 6-15 Entering sample information
Figure 6-16 How to select the patient gender
6-20
days.
“Days” box.
 Exit edit
changes.
6.9.2 Running the Samples

6-21

1. At the “Count” screen, be sure the System Status area displays “Ready“ and the Count
Mode area displays “PB“;
2. Present the mixed sample to the sample probe so that the tip is well into the tube, and
ID will automatically increase by 1 and the sample probe will be repositioned. And if the
auto print function is enabled, the analysis result will be automatically printed out;
5. Repeat the above steps on other samples.
6.9.3 Special Functions
Automatic saving of analysis results

This analyzer automatically saves a maximum of 10,000 sample results. When the maximum
number has been reached, the newest result will overwrite the oldest.
Parameter flags
 If the analysis result is followed by an ”H” or “L”, it means the analysis result has
exceeded the upper or lower limit of the reference range.
 If you see *** as opposed to the result, it means the result is either unreliable or
6-22
 If the WBC result is less than 0.5  109/L, this analyzer will not perform the
differential analysis and all the related parameter values will be non-numeric (***).
6-23
6.10 Shutdown
Perform the “Shutdown” procedure to shut down the analyzer daily.
 To ensure stable analyzer performance and accurate analysis results,

perform the “Shutdown” procedure to shut down the analyzer after it has
been running continuously for 24 hours.
 Shut down the analyzer strictly as instructed below.
1. Press [MENU] to enter the system menu and SELECT ”Shutdown”, as Figure 6-17
shows;
Figure 6-17 Selecting the shutdown program
2. A message box will pop up to ask you to confirm the shutdown, as Figure 6-18 shows;
Figure 6-18 Shutdown message box
3. CLICK “Yes” and a window will pop up to instruct you how to shut down the analyzer, as
Figure 6-19 shows;
6-24
Figure 6-19 Shutdown window (1)
4. Present the E-Z cleanser to the sample probe and press the aspirate key. The analyzer
will aspirate the E-Z cleanser and automatically clean the fluidic lines and the bath. The
cleaning progress will be displayed on the screen, as Figure 6-20 shows;
Figure 6-20 Shutdown progress bar
5. When the cleaning is finished, a window will pop up to instruct you what to do next, as
Figure 6-21 shows.
Figure 6-21 shutdown window (2)
6-25
6. Present the E-Z cleanser to the sample probe and press the aspirate key. The analyzer
will aspirate the E-Z cleanser and automatically clean the fluidic lines and the bath. The
cleaning progress will be displayed on the screen, as Figure 6-22 shows;
Figure 6-22 Shutdown progress bar
7. When the cleaning is finished, place the switch at the back of the analyzer to OFF (O) to
turn off the analyzer;
8. Empty the waste container.


 Fur and skin debris may block the aperture. Keep the sample clean before
using the analyzer to analyze it.
 Samples of the dogs infected with Filaria Larva in Blood may clog the
aperture.
6-26
7 Reviewing Sample Results
7.1 Introduction
The analyzer automatically saves analysis results. Totally 10,000 results can be saved. You
can either browse all the saved sample results in general or search for the results of a
particular sample or samples.
7-1
Reviewing Sample Results
7.2 Browsing All Sample Results
To browse all the saved sample results, you can choose either of the following modes:
 The ”Histogram” mode.
In this mode, you can review both parameter values and histograms of the saved sample
results, one sample result per screen.
 The “Table” mode.
In this mode, the sample results are presented in a columnar fashion without histograms
(namely you can only see the parameter values). One screen displays a maximum of 8
sample results.
7.2.1 Browsing in the Histogram Mode
Entering the “Histogram” mode

Press [MENU] to enter the system menu, and SELECT “Review” ( Figure 7-1 ) to enter the
“Review” screen ( Figure 7-2 ).
7-2
Figure 7-2 Sample histogram review screen
Browsing sample results

Press [←] or [→] to browse the preceding or following sample result; press [PgUp] or [PgDn]
to jump 8 locations (e.g. from location 1 to location 9).
Switching to the “Table” mode

To switch to the “Table” mode, press[↓]; to switch back to the “Histogram” mode,
press[↓]again.
Jumping to a sample result with known location

Press [F1] and a “Goto” window will pop up, as Figure 7-3 shows.
Figure 7-3 “Goto” window
ENTER the location into the “Location” box and press [ENTER] to jump to the desired
sample result.
Editing sample information

Press [F2] to edit the sample information, Figure 7-4 shows.
Figure 7-4 Editing sample information
 ID
You cannot edit the sample ID of an analyzed sample.
 Selecting the animal gender
SELECT the desired item from the “Gender” pull-down list. Note that you can select blank
7-3
in case you are not aware of the animal gender.
 Entering the animal name
ENTER the animal name into the “Animal box.
This analyzer provides three ways for you to enter the animal age – in years, in months and in
days. The first way is designed for the animals no younger than one year; the second for the
animals older than one month and younger than one year; the third for the animals younger
than one month. You can choose only one of the three ways to enter the animal age.
To enter the patient age in years: ENTER the desired number, an integer from 0 to 200, into
“Days” box.
 “Yes” button
to save the changes and return to exit the edit window.
 “No” button
If you do not want to save the entered information, CLICK the “Cancel” button to return to exit
the edit window.
Printing sample results

Press [PRINT] to print out the current sample result.
7.2.2 Browsing in the “Table” mode

At the “Histogram” mode, press[↓]to switch to the “Table” mode, as Figure 7-5 shows.
7-4
Figure 7-5 Sample table review screen
The sample results are sequentially displayed on the screen, The “Loc/Total” displayed in the
lower right corner of the screen indicates the location of the current sample result (the one
whose “ID” is backlit) and the total number of the sample results.

to browse the preceding or following screen.
Switching to the “Histogram” mode

If you are interested in reviewing the histograms of the current sample result, press[↓]to
switch to the ”Histogram” mode. To switch back to the “Table” mode, press [↓] again.

sample result.
You can select certain specific samples for transmission or printing.

 Selecting/deselecting a sample result
7-5
Press [←] or [→] to move the cursor to the specific sample result and press [ENTER] to select
it. The selected sample result will be marked with a “*”, as sample “118” in Figure 7-7 shows.
Figure 7-7 Selecting a sample result
Press [ENTER] again to deselect the sample result. Once the sample is deselected, the “*”
will disappear, as Figure 7-8 shows.
Figure 7-8 Deselecting a sample result
 Selecting/deselecting multiple sample results
Example1: To select the sample results of locations 1 to 5 (sample IDs: 114 to 118), follow the
procedure below to do so:
1. Press [F2] to enter the “Select” window, as Figure 7-9 shows;
7-6
Figure 7-9 Entering the “Select” window
2. ENTER the start position (“00001”) into the “From” box;
3. ENTER the end position (“00005”) into the “To” box;
4. CLICK “Select” and the lower left corner of the “Select” window will display “Select
samples”, as Figure 7-10 shows;
Figure 7-10 Selecting sample results of locations 1-5
5. CLICK “Exit” to return to the sample table review screen. The selected sample results
will be marked with “*”, as Figure 7-11 shows.
Figure 7-11 Reviewing the selected results
Example2: To deselect the sample results of locations 1 to 5, follow the procedure below to
do so:
1. Enter the start and end positions as instructed in steps 1 to 3 of Example1;
2. CLICK “De-select” and the lower left corner of the “Select” window will display
7-7
“De-select the results”, as Figure 7-12 shows;
Figure 7-12 Deselecting the sample results of locations 1 to 5
3. CLICK “Exit” to return to the sample table review screen. The “*” above those sample
results will disappear, as Figure 7-13 shows.
Figure 7-13 Reviewing the deselected results
Example3: To select the sample results of locations 1 to 5 and 7 to 8, follow the procedure
below to do so:
1. Select the sample results of locations 1 to 5 as instructed in steps 1 to 4 of Example1;
7-8
Example4:To deselect the sample results of locations 1 to 5 and 7 to 8, follow the procedure
below to do so:
1. Deselect the sample results of locations 1 to 5 as instructed in steps 1 to 3 of Example2;
3. CLICK “Quit” to return to the sample table review screen. The “*” above those sample
Transmitting sample results to a host

You can transmit the selected or all sample results to an external computer (a host). Press
[F3] to enter the “Transmit” window, as Figure 7-16 shows.
7-9
Figure 7-16 “Transmit” window
 To transmit the selected sample results to a host, CLICK “Selected”;
 To transmit all the sample results, CLICK “All”;
 To stop a transmission, CLICK “Stop”;
 To return to the sample table review screen, CLICK “Exit”.

Select the sample results you want to print and press [PRINT]. A message box will pop up to
ask you to confirm the printing, as Figure 7-17 shows. CLICK “Yes” to print out all the
selected results; CLICK “No” to abort the printing.
Figure 7-17 Print message box
7-10
7.3 Searching for Specific Sample Results
7.3.1 Starting a search

At the sample table review screen, press [F4] to enter the “Search” window, as Figure 7-18
shows.
Figure 7-18 “Search” window
To include a search condition, press [↑] or [↓] to move the cursor to the desired condition and
press [ENTER] to select the condition, as Figure 7-19 shows.
Figure 7-19 All search conditions are included
 Selecting the animal type
SELCT the desired animal type from the “Type” pull-down list.
ENTER the animal name into the “Animal” box.
7-11
 Entering sample ID
ENTER the ID number into the “ID” box.
 Entering the start and end date
ENTER the start date into the “Start” box; ENTER the end date into the “End” box.
CLICK “Yes” to start the search. The analyzer will search the saved sample results for
matches and report the conclusion at “Search Result” window, as Figure 7-20 shows. CLICK
“Yes” of the “Search Result” window to return to the searched sample review screen. The
matches found are saved in a database called “Searched” and you can review them in either
the “Table” mode or the “Histogram” mode.
Figure 7-20 “Search Result” window
7.3.2 Reviewing Search Result in the “Table” Mode
 For every search, the analyzer can display a maximum of 500 matches.
 The matches will be deleted if you have run another sample (including
background check), or deleted a sample result, or restarted the analyzer
after the search.
Entering the “Table” mode

At the “Review” screen, press [↑] to select the “Searched” database and then press [↓] to
enter the “Table” screen, as Figure 7-21 shows.
7-12
Figure 7-21 “Table” screen
The sample results are sequentially displayed on the screen, The “Loc/Total” displayed in the
lower right corner of the screen indicates the location of the current sample result (the one
whose “ID” is backlit) and the total number of the sample results matching the search
conditions.

to browse the preceding or following screen.
Switching to the “Histogram” mode

If you are interested in reviewing the histograms of the current sample result, press [↓] to
switch to the ”Histogram” mode. To switch back to the “Table” mode, press[↓] again.

7-13
sample result.
You can select certain specific samples for transmission or printing.

 Selecting/deselecting a sample result
Press [←] or [→] to move the cursor to the specific sample result and press [ENTER] to select
it. The selected sample result will be marked with a “*”, as sample “118” in Figure 7-23 shows.
Figure 7-23 Selecting a sample result
Press [ENTER] again to deselect the sample result. Once the sample is deselected, the “*”
will disappear, as Figure 7-24 shows.
7-14
Figure 7-24 De-selecting a sample result
 Selecting/deselecting multiple sample results
Example1: To select the sample results of locations 1 to 5 (sample IDs:114 to118 ), follow the
procedure below to do so:
1. Press [F2] to enter the “Select” window, as Figure 7-25 shows;
Figure 7-25 Entering the “Select” window
2. ENTER the start position (“00001”) into the “From” box;
3. ENTER the end position (“00005”) into the “To” box;
4. CLICK “Select” and the lower left corner of the “Select” window will display “Select
samples”, as Figure 7-26 shows;
Figure 7-26 Selecting sample results of locations 1 to 5
7-15
Example2: To deselect the sample results of locations 1 to 5, follow the procedure below to
do so:
1. Enter the start and end positions as instructed in steps 1 to 3 of Example1;
2. CLICK “De-select” and the lower left corner of the “Select” window will display
“De-select the result”, as Figure 7-28 shows;
Figure 7-28 Deselecting the sample results of locations 1 to 5
7-16
Example3: To select the sample results of locations 1 to 5 and 7 to 8, follow the procedure
below to do so:
Example4: To deselect the sample results of locations 1 to 5 and 7 to 8, follow the procedure
below to do so:
7-17
Transmitting sample results to a host

You can transmit the selected or all sample results to an external computer (a host). Press
[F3] to enter the “Transmit” window, as Figure 7-32 shows.
Figure 7-32 “Transmit” screen
 To transmit the selected sample results to a host, CLICK “Selected”;
 To transmit all the sample results, CLICK “All”;
 To stop a transmission, CLICK “Stop”;
 To return to the review screen, CLICK “Exit”.
7-18

7.3.3 Reviewing Search Result in the “Histogram” Mode
Entering the “Histogram” mode

At the “Review” screen, press [↑] to select the “Searched” database and then press [↓] to
enter the “Histogram” screen, as Figure 7-34 shows.
Figure 7-34 Searched histogram screen

to jump 8 locations (e.g. jumping from location 1 to location 8 ).
Switching to the “Table” mode

To switch to the “Table” mode, press [↓]; to switch back to the “Histogram” mode, press
7-19
[↓] again.

sample result.
Editing sample information

Press [F2] to edit the sample information, as Figure 7-36 shows.
Figure 7-36 Editing sample information
 ID
You cannot edit the sample ID of an analyzed sample.
ENTER the animal name into the “Animal box.
7-20
This analyzer provides three ways for you to enter the animal age – in years, in months and in
days. The first way is designed for the animals no younger than one year; the second for the
animals older than one month and younger than one year; the third for the animals younger
than one month. You can choose only one of the three ways to enter the animal age.
To enter the patient age in years: ENTER the desired number, an integer from 0 to 200, into
“Days” box.
 “Yes” button
to save the changes and return to exit the edit window.
 “No” button
If you do not want to save the entered information, CLICK the “No” button to return to exit the
edit window.

7-21
8 Using the QC Programs
8.1 Introduction
Quality Control (QC) consists of strategies and procedures that measure the precision and
stability of the analyzer. The results imply the reliability of the sample results. QC involves
measuring materials with known, stable characteristics at frequent intervals. Analysis of the
results with statistical methods allows the inference that sample results are reliable.
Mindray recommends you run the QC program daily. A new lot of controls should be
analyzed in parallel with the current lot prior to their expiration dates. This may be
accomplished by running the new lot of controls twice a day for five days using any empty QC
files. The QC files calculate the mean, standard deviation and coefficient of variation for each
selected parameter. The instrument-calculated means of these ten runs should be within the
expected ranges published by the manufacturer.

 Use the specified controls. Using controls other than the specified will lead
to misleading results.
 Refer to the instructions of use of the controls for how to store and use the
controls.
8-1
Using the QC Programs
8.2 QC Editing
8.2.1 Entering the “Quality control” screen
Press [MENN] to enter the system menu. SELECT “Quality control” (Figure 8-1), to enter
the “Quality control” screen, as Figure 8-2 shows. At the QC screen you can include a
maximum of 8 parameters, WBC, RBC, HGB, PLT, HCT, MCV, MCHC and MCH into a QC
run.
Figure 8-2 QC menu
8.2.2 Selecting a QC File

The analyzer provides 9 QC files for you to save QC settings and results. Every QC file can
save the results of a maximum of 31 QC runs. When the saved QC results have reached the
maximum number, the newest result will overwrite the oldest. You can press [F1] to switch the
QC files and the number will be given on the upper left of the screen.
8-2
8.2.3 Editing L-J Settings

If there are saved L-J results and settings, you need to delete them first. You can press [F5] to
enter “QC Table” screen to delete all the results, see Chapter 8.4.2 QC Table for details.
 Entering the “QC Edit” screen
Press [F3] at “Quality control” screen to enter the “QC Edit” screen (Figure 8-3).
Figure 8-3 “QC Edit” screen
 Entering lot number
ENTER the lot number of the control to be used into the “Lot No.” box, as Figure 8-4 shows.
Figure 8-4 Entering lot number
 Entering expiration date
ENTER the expiration date of the control to be used into the “Exp. Date” box, as Figure 8-5
shows.
8-3
Figure 8-5 Entering Exp. date
 Entering the expected results (mean) and limits (range)
ENTER the expected results (mean) and limits (range) respectively into the “Mean” and
“Range” boxes of the parameters to be included in the L-J analysis, as Figure 8-6 shows.
Figure 8-6 Entering Mean of RBC
 Refer to the instructions of use of the control for information on the lot
number, expiration date, open-vial stability days, expected results and limits.
 The entered expiration date should be either the expiration date printed on
the labeling or the open-vial expiration date, whichever is earlier.
 The open-vial expiration date is calculated as follows: the date that vial is
opened + the open-vial stability days.
 At the “QC Edit” screen, if you want to correct an erroneous entry, MODIFY
the wrong digit.
8-4
 Deleting settings
Press [DEL] to delete all the settings.
 Printing settings
Press [PRINT] to print out all the settings.
 Exiting the “QC Edit” screen
Press [MENU] to exit to the system menu. A message box shown in Figure 8-7 will pop up, if :
1. There is a parameter for which you have entered only the expected result or the limit; or
2. There is a parameter whose expected result is less than or equal to the limit.
Figure 8-7 invalid input
CLICK “Yes” to close the box and clear the erroneous entries. Re-enter the correct values
before trying to exit the screen again. The settings can be saved only when both the expected
result and limit are valid.
If all the entries are correct, a message box will pop up to remind you to save the changes, as
Figure 8-8 shows. CLICK “Yes” to save the changes and exit to the “Quality control” screen;
CLICK “No” to abort the changes and exit to the “Quality control” screen.
8-5
8.3 Running the Controls


8.3.1 Whole Blood Mode
1. Be sure the System Status area displays “Ready“;
2. Be sure the Analysis Mode area displays “WB“. If not, press [F2] to select the whole
blood mode;
3. Present a vial of control to the sample probe so that the tip is well into the vial, and press
the aspirate key. The System Status area will display “Running” and the analyzer will
start aspirating sample;
4. When you hear a beep and the sample probe is out of the vial, remove the vial. The
sample probe will retract into the analyzer and the analysis progress will be displayed on
the screen;
5. When the analysis is finished, the result will be displayed on the screen and the
“NO./Total” in the upper left corner of the screen will automatically increase by 1 and the
8-6
sample probe will be repositioned.

the corresponding error messages will be displayed in the lower left corner
of the screen and the results of all the related parameters will be invalidated.

results may be unreliable. See Chapter 11 Troubleshooting You Analyzer for
solutions.
 Deleting the QC Result
To delete the current result, press [DEL] and a message box will pop up, as Figure 8-9 shows.
CLICK “Yes” to confirm the deletion.
Figure 8-9 Deleting current result
 Printing QC results
Press [PRINT] to print out the current QC result by the printer.
 Exiting the “Quality control” screen
Press [MENU] to exit to the system menu.
8.3.2 Prediluted Mode
1. Be sure the System Status area displays “Ready“;
2. Be sure the Analysis Mode area displays “PB“. If not, press [F2] to select the prediluted
blood mode;
8-7
Figure 8-10 A message box showing you how to dispense diluent
key to dispense 0.7ml of diluent (the dispensing volume is controlled by the analyzer) into
the tube;
5. When the dispensing is finished, press [ENTER] to close the message box;
6. Add 20μL of control to the diluent and shake the tube to mix the sample;
7. Present the mixed control to the sample probe so that the tip is well into the tube, and
9. When the analysis is finished, the result will be displayed on the screen and the
“NO./Total” in the upper left corner of the screen will automatically increase by 1 and the
sample probe will be repositioned.
8-8

the corresponding error messages will be displayed in the lower left corner
of the screen and the results of all the related parameters will be invalidated.

results may be unreliable. See Chapter 11 Troubleshooting You Analyzer for
solutions.
 Deleting the QC Result
To delete the current result, press [DEL] and a message box will pop up, as Figure 8-12
shows. CLICK “Yes” to confirm the deletion.
Figure 8-12 Deleting current result
 Printing QC results
Press [PRINT] to print out the current QC result by the printer.
 Exiting the “Quality control” screen
8-9
8.4 Reviewing QC Results
You can review the saved results in either of the two modes – “L-J Graph” and “QC Table”.
8.4.1 L-J Graph

At the “Quality control” screen, press [F4] to enter the” L-J Graph” screen, as Figure 8-13
and Figure 8-14 shows.
Figure 8-13 L-J graph screen (1)
Figure 8-14 L-J graph screen (2)
The 8 parameters are divided into 2 groups for display, one group for one screen. You can
press [↑] or [↓] to switch among the screens. At every” L-J Graph” screen, you can press [←]
or [→] to view the results (displayed below the parameter box) of every point presented in the
8-10
graph. The current cursor position is displayed to the right of “No.” field and the time at which
this QC run was done is displayed to the right of ”Time” field.
The L-J graph is interpreted as follows:
 The x-coordinate represents how many times the QC program has been run. The
y-coordinate represents the analysis results of the displayed parameters.
 For every parameter, its L-J graph presents a maximum of 31 points.
 For every parameter, the upper dash line of its L-J graph represents the upper limit
of the expected range of the analysis result. The corresponding value (4.9 in case
of the WBC in Figure 8-13) equals Mean + Range and is displayed to the left of the
line.
 For every parameter, the lower dash line of its L-J graph represents the lower limit
of the expected range of the analysis result. The corresponding value (4.1 in case
of the WBC in Figure 8-13) equals Mean - Range is displayed to the left of the line.
 For every parameter, its expected result (4.5 in case of the WBC in Figure 8-13) is
displayed between the values of the upper dash line and of the lower dash line.
 For every parameter, the three numbers displayed to the right of its L-J graph
represents:
“Mean” – the mean value of the saved results, as the equation below defines,
X i
Mean  i 1
n
where n represents how many times the QC program has been run and Xi is the result
acquired from every QC analysis.
“Diff” – standard deviation of the saved analysis results, as the equation below defines,
Diff 

 X i  Mean 2
n 1
where n represents how many times the QC program has been run and Xi is the result
acquired from every QC analysis and “Mean” is the mean value derived from the first
equation.
“CV” – Coefficient of Variation, as the equation below defines
Diff
CV   100%
Mean
where Mean is the mean value derived from the first equation and Diff is the standard
deviation derived from the second equation.
 Every point in the graph is interpreted as follows:
8-11
The darkened square ■ that falls between the upper and the lower dash lines is within the
control range. Otherwise, it is not. The blank square □ represents the QC analysis either ran
into errors or is out of the display range.
If you see any points fallen outside the control range, do the following steps until the problem
is solved. If all the steps have failed, contact Mindray customer service department or your
local distributor for assistance.
1. Check the lower left corner of the screen for error messages. Refer to Chapter 11
Troubleshooting Your Analyzer for solutions to any displayed error messages;
2. Check the L-J settings for inappropriate entries;
3. Do the background check. In case of an abnormal background result, refer to Chapter 11

Troubleshooting Your Analyzer for solutions;
4. Re-run the control;
5. Run another vial of control;
6. Check if the analyzer needs to be calibrated.
 Other operations:
To print out the currently displayed L-J graph, press [PRINT]. To acquire help information,
press [HELP]. To return to the ”Quality control” screen, press [MENU].
8.4.2 QC Table
At the “Quality control” screen, press [F5] to enter the “QC Table” screen, as Figure 8-15
shows, where every screen displays the results of 6 QC analyses. You can press [PgUp] or
[PgDn] to switch to the previous or next screen to view other results.
Figure 8-15 “QC Table” screen
8-12
If you want to delete all the saved results, press [DEL] and a message box will pop up to
confirm the deletion, as Figure 8-16 shows.
Figure 8-16 Deleting all the result
CLICK “Yes” to delete current result; CLICK “No” to abort the deletion.
If you want to transmit the saved QC results to an external computer, follow the steps given
below:
1. Press [F1] at “QC Table” screen to enter the dialog box shown in Figure 8-17;
2. SELECT “Yes” to confirm the transmission.
Figure 8-17 Transmission dialog box
8-13
9 Using the Calibration Programs
9.1 Introduction
The purpose of the calibration is to maintain system accuracy. Quality of the calibration
depends on the calibration materials and reagents used. You should only use the calibrators
and reagents specified by Mindray for the calibration. Store and use the calibrators and
reagents as directed by their instructions for use.
9-1
Using the Calibration Programs
9.2 When to calibrate
You should run the calibration program if the quality control results indicate there may be a
problem.
 All of the measured parameters must be calibrated before readings of this

analyzer can be used as valid analysis results.
9-2
9.3 How to Calibrate
The analyzer provides 2 calibration programs: manual calibration and auto calibration using
commercial calibrators. In addition, every type of animal has its own set of calibration factors.
9.3.1 Preparing Your Analyzer

Do the following pre-calibration procedures before calibration. If problems are detected
during these checks, do not attempt to calibrate the analyzer. If necessary, call Mindray
customer service department or your local distributor for assistance.
Check and make sure there are enough reagents for the calibration.
Do the background check. If the analyzer alarms for abnormal background results, see
It is recommended that you create a log table for your analyzer. This log table should contain
all necessary information that is pertinent to your analyzer. Suggested items that you may
want to include in the log table are:
 Calibration date
 Supplier of calibrator
 Lot number
 Expected results and limits
 Result of background check.
Enter the administrator password as instructed in Chapter 5.2.1 and then choose one or
several parameters among WBC, RBC, HGB, MCV and PLT for calibration.
9-3
9.3.2 Auto Calibration Program
The new calibration factors obtained though auto calibration will be applied to all animal
species.
SELECT “Calibration→ Auto” (Figure 9-1) to enter the “Auto” screen (Figure 9-2).
Figure 9-2 “Auto” screen
Selecting the count mode

Press [F1] to select desired calibration mode.
Editing calibration settings

Press [F2] to activate the edit boxes.
 Entering lot number
9-4
ENTER the lot number of the calibrator to be used into the “Lot No.” box.
 Entering Exp. Date
ENTER the expiration date of the calibrator to be used into the “Exp. Date” box.
 Entering the expected results (mean) and limits (range)
ENTER the expected results (mean) into the “Mean” box of the parameters to be included in
the calibration.
 Refer to the instructions of use of the calibrators for information on the lot
number, expiration date, expected results and limits.
 Open reagents are stable for 60 days. The entered expiration date should be
the open date + 60 days or the expiration date marked on the packaging of
the reagent, whichever is earlier.
 When editing the settings, if you want to correct an erroneous entry,

MODIFY the wrong digit.
 Exit editing
When you have finished editing the desired settings, press [F2] to deactivate the edit boxes.
Running the calibrator
 Use the Mindray- specified calibrator. Using calibrator other than the
specified will lead to misleading results.
 Refer to the instructions of use of the calibrator for how to store and use the
calibrator.
 In the prediluted calibration mode, you cannot dispense diluent from the
analyzer. It is recommended that you prepare at least 7 cups of diuent before
starting calibrating the analyzer in the prediluted mode.
9-5

 Do not re-use such disposable products.
 In the whole blood mode
1. At “Auto” screen, press [F1] to select “Whole Blood” mode;
2. Present a vial of mixed calibrator to the sample probe so that the tip is well into the tube,
and press the aspirate key and the analyzer will start aspirating sample;
3. When you hear the beep and the sample probe is out of the vial, remove the calibrator.
The sample probe will retract into the analyzer and the analysis progress will be
probe will be repositioned.
 In the prediluted mode
1. Press [MENU] and SELECT “Count” to enter the “Count” screen;
2. At “Count” screen, be sure the System Status area displays “Ready“ and the Count
Mode area displays “PB“;
Figure 9-3 “Add Diluent” window
key to dispense 0.7mL of diluent (the dispensing volume is controlled by the analyzer)
9-6
into the tube. Prepare at least 7 cups of diluent;
5. When the dispensing is done, press [ENTER] to close the message box;
6. Make sure the calibrator is at room temperature and not expired;
7. Add 20μL of calibrator into one of the prepared sample cups and mix them well;
8. Present the mixed calibrator to the sample probe;
9. Press the aspirate key to start the run. When you hear a beep and the sample probe is
out of the cup, remove the sample;
10. Press [MENU] and SELECT “Calibration → Auto”;
11. Press [F1] to select the “Prediluted” mode;
12. Press [F2] to activate the edit boxes and edit the settings as needed. When the editing is
done, press [F2] again to deactivate the edit boxes;
13. At the “Auto” screen, repeat steps 6 to 9 for 5 consecutive times.


the corresponding error messages will be displayed and the results of all the
related parameters will be invalidated. See Chapter 11 Troubleshooting Your

solutions.
9-7
Saving the calibration results

If non-numeric parameter values (“***”) are obtained, a message box will pop up to warn you,
as Figure 9-5 shows. CLICK “Yes” to close the dialog box and discard the result.
Figure 9-5 A message box to warn you about the invalid results
If the parameter values obtained are numeric, a message box will pop up to ask you to
confirm whether the obtained calibration result is valid. Figure 9-6 shows the message box
asking whether you think the result of the first calibration (calibration 1) is valid..
Figure 9-6 A message box to confirm the validity
You can either CLICK “Yes” to save the results, or CLICK “No” to abort the result. The saved
results will be displayed on the screen.
Repeat the above steps to run the calibrator 3 to 5 times (5 is recommended) and the
analyzer will automatically calculate the CVs and calibration factors, as Figure 9-7shows.
Figure 9-7 Results of the auto calibration
The calculated calibration factor should be within the 75％ to 125％. If not, there will be
flagged with a “*”. Other values will not be displayed. In case of an empty calibration factor, try
to find out the reason and if necessary, contact Mindray customer service department or
your local distributor for assistance.
9-8
Press [MENU] to exit the “Auto” screen, a message box will pop up to confirm the new
calibration factors, as Figure 9-8 shows.
Figure 9-8 A message box to confirm the new calibration factors
CLICK ”Yes” to save the new calibration factors. Click “No” to close the box without saving
the changes. .
Other operations
 Printing new calibration factors
Press [PRINT] to print out the current calibration factors.
 Exiting the “Auto ” screen
Press [MENU] to exit to the system menu
9-9
9.3.3 Manual Calibration Program

You should first enter the administrator password “2826”at the “Setup“→ “Password” screen.
Selecting animal
Every type of animal has its own set of calibration factors. Select the right animal type before
proceeding with the calibration.
Press [MENU] to enter the system menu, as Figure 9-9 shows;
SELECT “Animal” to enter the “Animal” screen, as Figure 9-10 shows;
Figure 9-10 “Animal” screen
SELECT the desired animal;
Editing the new calibration factors

9-10
SELECT “Calibration → Manual” (Figure 9-10) to enter the “Manual” screen ( Figure 9-11).
Figure 9-11 “Manual” screen
The left of the “Manual" screen displays the available calibration modes – “Whole blood”
and “Prediluted”. The right of the “Manual” screen displays the calibration factors of WBC,
RBC, HGB, MCV, PLT and the time the factors are saved.
Press [F2] to activate the edit boxes as Figure 9-12 shows.
Figure 9-12 Edit boxes activated
ENTER the new calibration factor into the corresponding boxes.
Exit editing
Press [F2] to exit editing.
If the entered number is out of the calibration range, a dialog box will pop up to remind you
the entered number is invalid, as Figure 9-13 shows.
9-11
Figure 9-13 A message box to warn invalid input
Seeing the box, CLICK “Yes” and re-enter the factors. If the changed factors are all within the
calibration range, a dialog box will pop up to remind you to save the new factors, as Figure
9-14 shows.
Figure 9-14 A message box to confirm the new calibration factors
CLICK ”Yes” to save the new calibration factors, and then a dialog box will pop up to ask you
whether to apply the factors to all animal species, as Figure 9-15 shows.
Figure 9-15 A message box to confirm the application of the new calibration factors
Click “Yes” to apply the new calibration factors to all animal species. Click ”No” to close the
box without applying.
Other operations
 Printing new calibration factors
9-12
Press [PRINT] to print out the current calibration factors.
 Exiting the “Manual” screen
9-13
10 Maintaining Your Analyzer
10.1 Introduction
Preventive and corrective maintenance procedures are required to keep the BC-2800Vet in a
good operating condition. This analyzer provides multiple maintenance functions for this
purpose. This chapter introduces how to use the provided functions to maintain and
troubleshoot your analyzer.
 Do not perform any maintenance procedures that are not described in this
chapter. Performing unauthorized maintenance procedures can damage
your analyzer.
 In case of problems not specified in this manual, contact Mindray customer

service department or your local distributor for assistance.
 Only Mindray-supplied parts can be used for maintenance. For any

questions, contact Mindray customer service department or your local
distributor.
10-1
Maintaining Your Analyzer
10.2 General Guidelines
Maintenance Period Content of Maintenance
Everyday If you are to use this analyzer 24 hours a day, perform the “E-Z
cleanser cleaning” procedure everyday.
Run the QC program everyday. See Chapter 8 Using the QC
Programs for details.
Every three days If you are to use this analyzer 24 hours a day, perform the “Probe
cleanser cleaning” procedure every three days.
Every Week If you shut down your analyzer every day and follow the specified
shutdown procedure to do that, you need to perform the “Probe
cleanser cleaning” procedure every week.
Every Month You should use the supplied probe localizer to calibrate the
position of the probe to that of the probe wipe. The analysis result
is sensitive to their alignment.
As needed When you think the bath might be contaminated, perform the
“Clean the bath” procedure.
When the analyzed samples add up to 100, the analyzer will
remind you to perform the “Probe cleanser cleaning” procedure.
When the analyzed samples add up to 100, the analyzer will
remind you to perform the “E-Z cleanser cleaning” procedure.
When the analyzed samples add up to 4,000, the analyzer will
remind you to perform the “Clean wipe block” procedure.
When this analyzer is not to be used for two weeks, perform the
“Prepare to ship” procedure to empty and wash the fluidic lines
and then wipe the analyzer dry and wrap it up for storage.
To obtain reliable analysis results, this analyzer needs to work in a
normal status. Run the “Self-test” items regularly to check the
status of this analyzer.
When this analyzer gives alarms for clogging, you can perform the
“Flush aperture” or “Zap aperture” procedure, or press [F2] to
unclog the aperture.
If you see other error messages, see Chapter 11
Troubleshooting Your Analyzer for solutions.
10-2
10.3 Using the “Maintenance” Program
Press [MENU] to enter the system menu. SELECT “Service → Maintenance” (Figure 10-1)
to enter the “Maintenance” screen (Figure 10-2).
Figure 10-2 “Maintenance” screen
10-3
There are 13 maintenance procedures available at the “Maintenance” screen.
 Diluent Prime
 Rinse Prime
 Lyse Prime
 Zap Aperture
 Flush Aperture
 Probe Cleanser Cleaning
 E-Z Cleanser Cleaning
 Lyse Test
 Clean Bath
 Drain Bath
 Drain Tubing
 Clean Wipe Block
 Prepare to ship
10.3.1 Diluent Prime
doctor.
 After installing a new container of diluent, rinse or lyse, do a background

check to ensure the background results are normal.
You should perform the “Diluent prime” procedure to prime the diluent tubing when
 there are bubbles in the tubing; or
10-4
 the diluent in the tubing is contaminated; or
 you have installed a new container of diluent without shutting off the analyzer.
At the “Maintenance” screen, SELECT “Diluent prime” to prime the tubing and the priming
progress will be displayed at the bottom of the screen, Figure 10-3 shows. When the priming
is done, the screen will return to the initial state.
Figure 10-3 Priming diluent screen
10.3.2 Rinse Prime
doctor.

You should perform the “Rinse prime” procedure to prime the rinse tubing when
10-5
 the rinse in the tubing is contaminated; or
 you have installed a new container of rinse without shutting the analyzer.
At the “Maintenance” screen, SELECT “Rinse prime” to prime the tubing and the priming
progress will be displayed at the bottom of the screen, as Figure 10-4 shows. When the
priming is done, the screen will return to the initial state.
Figure 10-4 Priming rinse screen
10.3.3 Lyse Prime


You should perform the “Lyse prime” procedure to prime the lyse tubing when
10-6
 the lyse in the tubing is contaminated; or
 you have installed a new container of lyse without shutting the analyzer.
At the “Maintenance” screen, SELECT “Lyse prime” to prime the tubing and the priming
priming is done, the screen will return to the initial state.
Figure 10-5 Priming lyse screen
10.3.4 Zap Aperture

You can perform the “Zap aperture” procedure to unclog the apertures or prevent clogging.
At the “Maintenance” screen, SELECT “Zap aperture” to zap the apertures and the zapping
zapping is done, the screen will return to the initial state.
10-7
Figure 10-6 Zapping aperture
10.3.5 Flush Aperture

You can perform the “Flush aperture” procedure to flush the aperture to unclog the aperture
or prevent clogging.
At the “Maintenance” screen, SELECT “Flush aperture” to flush the aperture and the
flushing progress will be displayed at the bottom of the screen, as Figure 10-7 shows. When
the flushing is done, the screen will return to the initial state.
Figure 10-7 Flushing aperture
10-8
10.3.6 Probe Cleanser Cleaning

 The probe cleanser is corrosive. Wear proper personal protective equipment

(e.g. gloves, lab coat, etc.) and follow safe laboratory procedures when
handling them in the laboratory.
You can soak the bath and fluidic lines with the probe cleanser, an alkaline detergent, by
performing the “Probe cleanser cleaning” procedure. If your analyzer is to run 24 hours a
day, you should perform this procedure every 3 days. If you follow the shutdown procedure to
turn off your analyzer everyday, you should perform this procedure every week.
Follow the steps given below to do so:
1. At the “Maintenance” screen, SELECT “Probe cleanser cleaning”;
2. Present the cleanser to the probe and press [ENTER] to aspirate the cleanser. When you
hear the beep and the sample probe is out of the bottle, remove the cleanser. The
analyzer will start priming process, as Figure 10-8 shows;
Figure 10-8 Priming bath and fluidic lines
3. When the priming is done, the analyzer will start the 5-minute soaking process, as Figure
10-9
10-9 shows and you may press [ENTER] to stop it before the time is due. Note that a
shortened priming process may not be as effective as a complete one;
Figure 10-9 soaking process
4. When the soaking is done, the analyzer will start the cleaning process, as Figure 10-10
shows, after which screen will return to the initial state;
Figure 10-10 Cleaning process
To make sure this analyzer functions normally, every time the accumulated analyzed samples
reach 100, a message box will pop up to remind you to perform the “probe cleanser
cleaning” procedure, as Figure 10-11 shows. CLICK “Yes” to proceed with the cleaning;
CLICK “No” to cancel the cleaning.
10-10
Figure 10-11 A message box to confirm the cleaning
10.3.7 E-Z Cleanser Cleaning

You can use the E-Z cleanser, an enzyme based, isotonic cleaning solution and wetting agent,
to clean the tubing and bath by performing the “E-Z Cleanser Cleaning” procedure.
Follow the steps given below to perform the procedure:
1. At the “Maintenance” screen, SELECT “E-Z Cleanser Cleaning”;
2. Present the cleanser to the probe and press [ENTER] to aspirate the cleanser. When you
hear the beep and the sample probe is out of the bottle, remove the cleanser. This
analyzer will automatically prime the bath and fluidic lines with the aspirated cleanser and
the progress is displayed on the screen, as Figure 10-12 shows;
Figure 10-12 Priming the bath and fluidic lines
3. When the priming is done, the analyzer will start the 10-minute soaking process, as
Figure 10-13 shows.
10-11
Figure 10-13 E-Z cleaning
4. When the soaking is done, the analyzer will start the draining process, as Figure 10-14
shows. When the draining is done, the whole procedure is over and the screen will return
to the initial state.
Figure 10-14 Draining the bath and fluidic lines
If your analyzer has been running continuously for 24 hours, a dialog box, as Figure 10-15
shows, will pop up to remind you to perform the “E-Z cleanser cleaning” procedure. If you
want to do so, CLICK “Yes “. Otherwise, CLICK “No” to cancel the cleaning.
10-12
Figure 10-15 A message box to confirm the cleaning
10.3.8 Lyse Test
doctor.
In case of any abnormal WBC counts or histograms, you can perform the “Lyse test”
procedure to check whether the lyse can be dispensed properly.
1. Unscrew and remove the retaining screws (pointed to by the arrows shown in Figure
10-16) on the right plate;
Figure 10-16 Removing the two screws
2. Follow the arrow shown in Figure 10-17 to push and remove the right plate;
10-13
Figure 10-17 Removing right plate
3. Remove the screws fixing the shielding box of the bath, as Figure 10-18 shows;
Figure 10-18 Shielding box
4. Remove the shielding box to expose the bath, as Figure 10-19 shows;
10-14
Figure 10-19 Bath
5. SELECT “Lyse test”. The analyzer will automatically drain the bath and then dispense
2ml of lyse into the bath;
6. Check the scale to see whether the lyse has reached the expected line (the second from
the bottom). If so, press [ENTER] and the analyzer will automatically flush the bath and
dispense lyse and the test is done;
7. If not, repeat steps 5 and 6 several times. If all the tries have failed, check whether the
lyse has run out or the lyse pickup tube is not properly connected to this analyzer. If there
is still enough lyse and the tube is well connected to the analyzer, contact the Mindray or
10.3.9 Clean Bath

Follow the steps given below to perform the “Clean bath” procedure:
SELECT “Clean bath” to start the cleaning procedure, as Figure 10-20 shows. When the
cleaning is done, the screen will return to the initial state;
10-15
Figure 10-20 Clean bath
10.3.10 Drain Bath

When three or more of the WBC, RBC, PLT and HGB results are abnormal, you may do the
“Drain bath” procedure to determine the reason.
Follow the steps below to do so:
1. Do steps 1 to 4 of the “Lyse test” procedure to expose the bath;
2. SELECT “Drain bath” to drain the bath;
Figure 10-21 Draining the bath
3. When the draining is done, check the bath and the tubing below for residual fluid. If there
is no fluid, press [ENTER] to prime the bath with diluent, as Figure 10-22 shows. To
prevent you from forgetting to prime the bath, a time box will pop up as a reminder;
10-16
Figure 10-22 The time of draining bath
Figure 10-23 Priming the bath with diluent
4. When the priming is done, the screen will return to the initial state;
5. If there is fluid left, turn off the analyzer and call Mindray customer service department or
10-17
10.3.11 Drain Tubing
doctor.
 Do the “Drain Tubing” procedure before relocating the analyzer.
You can perform the “Drain tubing” procedure to drain the fluidic system. Follow the steps
given below to do so:
1. Press the appropriate arrow keys ([←][→] [↑][↓]) as needed to move the cursor to “Drain
tubing”;
2. Remove the diluent, rinse and lyse pickup tubes from the back of the analyzer;
3. Press [ENTER] to start the draining process, as Figure 10-24 shows;
Figure 10-24 Draining the fluidic lines
4. When the draining is done, the screen will display “Turn off this analyzer” and you
should turn off the power switch.
10-18
10.3.12 Clean Wipe Block

After being used for a long time, the bottom of the probe wipe may be contaminated by blood
and the inside of the probe wipe may also be contaminated by the dirt sucked in. So you need
to clean the probe wipe regularly.
1. Present the probe cleanser to the sample probe;
2. SELECT “Clean wipe block” to start the aspiration;
3. When you hear the beep and the sample probe is out of the bottle, remove the cleanser;
4. Unscrew and remove the retaining screws (pointed to by the arrows shown in Figure
10-25) on the right plate;
10-19
6. Follow the instructions displayed on the screen to place an empty cup, whose diameter
should be no less than 8cm, below the sample probe;
7. Press [ENTER] to soak the wipe block with the aspirated cleanser. The soaking progress
will be displayed on the screen, as Figure 10-27 shows;
Figure 10-27 Cleaning wipe block
8. When the soaking is done, wipe the bottom of the wipe block with a probe
cleanser-dipped cloth that does not leave debris;
9. Press [ENTER] to flush the block and the interior of the probe and the flushing progress is
displayed on the screen, as Figure 10-28 shows;
Figure 10-28 Flush the block and the interior of the probe
10. After the flushing is done, the screen returns to the initial state.
10-20
When the accumulated analyzed samples reach 4,000, a message box will pop up to remind
to clean the probe wipe, as Figure 10-29 shows. CLICK “Yes” to do the procedure; CLICK
“No” to abort the procedure.
Figure 10-29 message box
10.3.13 Prepare to ship

Use the “Prepare to ship” program to prepare your analyzer for a prolonged period of
non-use or for shipping.
1. Press the appropriate arrow keys ([↑][↓][←][→]) to move the cursor to ”Prepare to
ship”. Remove the diluent, rinse and lyse tubing from the containers following the
instructions displayed on the screen;
2. Press [ENTER] and a message box will pop up to ask you to confirm this operation, as
Figure 10-30 shows;
Figure 10-30 A message box of prepare to ship
3. CLICK “No” if you want to abort this operation; CLICK “Yes” to proceed with the
operation. The analyzer starts to drain the fluidic lines and the progress is displayed on
the screen, as Figure 10-31 shows.
10-21
Figure 10-31 Draining fluidic lines
4. After draining the tubing, follow the instructions displayed on the screen (Figure 10-32) to
put the rinse, diluent and lyse tubing into distilled water and press [ENTER] to flush this
analyzer with the distilled water;
Figure 10-32 Washing the analyzer
5. When the washing is over, follow the instructions displayed on the screen to remove the
rinse, diluent and lyse tubing from the distilled water and press [ENTER] to drain the
tubing again;
6. Turn off the analyzer when the screen displays “Turn off the analyzer”;
7. Wipe this analyzer dry and wrap it up for storage.
10-22
10.4 Using the “Status” Program
The items displayed in the “System Status” screen reflect how the analyzer is functioning
and contribute significantly to diagnosing analyzer errors. You may follow the instructions
given below to check those items.
Press [MENU] to enter the system menu and SELECT “Service → Status”, as Figure 10-33
shows, to enter the “Status” screen, as Figure 10-34 shows.
Figure 10-34 “Status” screen
At the “Status” screen you can only view the displayed status information and reference
ranges.
10-23
10.5 Using the “Self-test” Program

The system self-test is a major way to locate system errors. Follow the instructions given
below to view and check the available self-test items.
Press [MENU] to enter the system menu and SELECT “Service → Self-test”, as Figure
10-35 shows, to enter the “Self-test” screen, as Figure 10-36 shows.
Figure 10-36 “Self-test” screen
This screen can be interpreted as follows:
 Test Groups area (on the left)
This area displays the test groups. The available self-test items are divided into four groups,
“Tubing”, “Machine”, “Valve” and “Circuit”.
Press [F1] to select the desired group. The selected group is preceded by a ⊙.
10-24
 Test Result area (on the right)
This area displays the items included in the test group and the test results.
 Help area (on the bottom)
This area displays useful information to help you move to the next step.
At this screen, if you want to acquire help information, press [HELP]; if you want to print out
the test results (except for the results of the valve tests), press [PRINT].
10.5.1 Testing the fluidic system

Press [F1] to select the “Tubing” group. To conduct the following tests, just SELECT the
desired test and the results will be displayed later.
 Count Time
It measures the duration of a WBC and RBC count, namely how many seconds it takes for
the aspirated fluid flows from the first sensor to the second.
 Aperture(v)
It measures the voltage (v) over the aperture.
 Vacuum
It checks whether the vacuum system functions normally.
 Pressure
It checks whether the system flushes the aperture at a normal pressure.
 Filter
It checks whether the filter functions normally.
10.5.2 Testing Motors and Recorder/Printer

To test the motors and recorder/printer, press [F1] to select the “Machine” group, as Figure
10-37 shows.
10-25
Figure 10-37 testing mechanic part
To conduct the following tests, SELECT the desired test and the results will be displayed
later.
 Syringe motor
The syringe motor controls the aspiration volume. This test checks whether the motor
functions normally.
 Rotation motor
The rotation motor rotates the sample probe inside the analyzer. This test checks whether the
motor functions normally.
 Elevator motor
The elevator motor controls elevation of the sample probe. This test checks whether the
motor functions normally.
 Print
This test checks whether the recorder or printer functions normally. If normal, when you press
[ENTER], the recorder or printer will print out a test page; if abnormal, the screen will display
the corresponding error message and you can see Chapter 11 Troubleshooting Your
10.5.3 Testing Valves

To test whether the valves function properly, press [F1] to select the “Valve” group, as Figure
10-38 shows. Follow the steps below to test the desired valve.
10-26
Figure 10-38 Testing valves
To test a valve, SELECT the valve. If the valve goes through an Off-On-Off sequence without
making any abnormal sound, it passes the test. Otherwise, something may be wrong with the
valve.
10.5.4 Testing A/D Interrupt

To test the A/D interrupt, press [F1] to select the “Circuit” group, as Figure 10-39 shows.
Figure 10-39 Testing A/D interrupt
To conduct the test, SELECT “A/D interrupt” and the test result will be displayed later.
10-27
10.6 Log
The log records all the major events taking place during the running of this analyzer. It helps
the service engineers diagnose system errors.
Press [MENU] to enter the system menu and SELECT “Service→Log”, as Figure 10-40
shows to enter the “Log” screen, as Figure 10-41 shows.
Figure 10-41 “Log” screen
The recorded events are divided into three groups, “All”, “Settings” and “Other”( including
setting discriminators, system self-test and updating system software), which are all listed on
the left of the screen. All the recorded events are listed on the right of the screen by default.
You can press [F1] to select the specific group and the right of the screen will display the
events of the selected group only. Every screen displays 10 events. You can press [↑] or [↓]
to check the events one by one or press [PgUp] or [PgDn] to check the events on the
previous or next screen. If you want to print out the displayed events, press [PRINT]. If you
want to acquire help information, press [HELP].
10-28
For every recorded event, the “NO.” column displays the sequences of the recorded events;
the “Time” column displays the time when this event occurred; the “Type” column displays
the event type; the “Times” column displays how many times (1 to 255) this event occurred
and if it occurred more than 255 times, the excessive events will be recorded from 1 to
another log file; the “Information” column displays extra information regarding the event.
This analyzer can save a maximum of 1000 log files and once the maximum number has
been reached, the newest log will automatically cover the oldest one.
10-29
10.7 Viewing System Configuration
To view the system configuration, press [MENU] to enter the system menu, and SELECT
“Service→Config.” , as Figure 10-42 shows, to enter the “Config.” screen, as Figure 10-43
shows.
Figure 10-42 system menu
Figure 10-43 “Config.” screen
Every screen displays 13 items and you can press [↑] or [↓] to select the item you want to
see, or press [PgUp] or [PgDn] to go to the previous or next screen. If you want to print out
the configuration, press [PRINT]. If you want to acquire help, press [HELP].
10-30
10.8 Printing Management
Press the [MENU] to enter the system menu and SELECT “Service→Print.” , as Figure
10-44 shows, to enter the “Print” screen, as Figure 10-45 shows.
Figure 10-45 “Print” screen
The printing tasks are queued in this screen, where you can view the all and delete those
waiting to be processed. Once something goes wrong with the printing device, the task being
processed will be deleted and the queued tasks will keep waiting. Once the system finds the
error has been removed, it will resume printing and process the tasks from the first one. Note
that you cannot change the sequence of the queued tasks.
You can perform the following operations at the “Print” screen:
 Press [DEL] to delete the selected task;
 Press [HELP] to display the help information;
 Press [MENU] to return to the system menu.
10-31
10.9 Calibrating Sample Probe Position

The relative position between the sample probe and probe wipe block has influence on the
analysis results. In the accessory box, there is a sample probe localizer, as Figure 10-46
shows. You need to use the localizer to adjust the position of the sample probe if you have
replaced wipe block, or observed motor error, or incorrect result. Also, as required by regular
maintenance, you should use the localizer to adjust the position of the sample probe monthly.
Figure 10-46 Probe localizer
1. SELECT “Setup → Password” and enter the administrator password;
2. Unscrew and remove the retaining screws with hands or screwdrivers (pointed by the
arrows shown in Figure 10-47) on the right plate.
3. Follow the arrow shown into push and remove the right plate;
10-32
4. Press [F1] to select the “Machine” group and SELECT “Elevator motor”, as Figure
10-49 shows;
Figure 10-49 “Self-test” screen
5. An “Elevator motor” window will pop up, as Figure 10-50 shows;
10-33
Figure 10-50 “Elevator motor” window
6. Press [↑] to move the sample probe upward and press [→] to move the probe to above
the bath, as Figure 10-51 shows;
Figure 10-51 Sample probe above the bath
7. Loose the retaining screw by a screwdriver, as Figure 10-52 shows.
10-34
Figure 10-52 Removing screws
8. Remove the probe from the wipe block and insert the localizer into the wipe block from
the bottom, as Figure 10-53 shows;
Figure 10-53 Using localizer
9. Insert the probe into the wipe block until it reaches the localizer, as Figure 10-54 shows;
Figure 10-54 Inserting sample probe into wipe block
10. Retighten the screw to fix the probe and remove the localizer to complete the adjustment.
10-35
10.10 Replacing the Probe Wipe

To replace the probe wipe:
1. Refer to Chapter 10.9 and do the steps 1 to 8;
2. Pull the loosen probe wipe upward to remove the wipe block and disconnect the tubes
from the wipe block (pay attention to the correspondence between the tubes and the
connectors), as Figure 10-55 shows;
Figure 10-55 Installing wipe block
3. Install a new block and connect the tubing end with the black marking to the connector
below the block;
4. Calibration to the sample probe position should be performed after replacing the probe
wipe. Refer to Chapter 10.9..
10-36
10.11 Replacing the Filter of the Vacuum Chamber
1. Unscrew and remove the retaining screws with hands or screwdrivers (pointed by the
arrows shown in Figure 10-56 ) on the right plate;
Figure 10-57 Removing the right plate
3. Find the filter shows in Figure 10-58;
10-37
Figure 10-58 Vacuum filter
4. Remove the filter and take a new one from the accessory kit and install it.
10-38
10.12 Maintaining Recorder
 Wear proper personal protective equipment (e.g. medical gloves, disposable

wrist strap, etc.) against the static electricity during maintaining recorder.
 Make sure the power supply of the analyzer has been cut off before
maintenance.
 If the recorder finishes a print action, wait at least 20 minutes until the
recorder head is cooled completely and then maintain the recorder.
 Do not add unnecessary force to the recorder head.
 Wipe off the alcohol remaining on the recorder head in time after cleaning
the recorder head.
 Make sure the alcohol volatilizes completely before using the recorder to
print.
 Maintain the recorder strictly as instructed below.
You should maintain the recorder every two months. Do as follows to maintain your recorder:
1. Turn off the analyzer and cut off the power supply;
2. Open the recorder door and take out the recorder paper;
3. Gently wipe the roller from left to right using cotton swabs;
4. Roll the roller and repeat step 3 to clean off all debris and stains on the roller;
5. Gently wipe the heating part of the recorder head from left to right using cotton swabs
dipped with alcohol (no drops) to clean off all debris and stains on the head;
6. Wipe off the alcohol remaining on the heating part of the recorder head using dry cotton
swabs;
7. Wait at least 20 minutes till alcohol on the heating part of the recorder head volatilizes
completely, and then install recorder paper and close the recorder door.
10-39
Figure 10-59 Heating part of recorder head and roller
See the figure above to find the heating part of the recorder head and the roller when the
recorder door is opened.
10-40
11 Troubleshooting Your Analyzer
11.1 Introduction
The BC-2800Vet continuously monitors the status of the system and displays pertinent
information in the lower left corner of the “Count” screen (the Error Message area). If a
problem is detected, the Error Message area displays the corresponding error message. This
chapter contains information that is helpful in locating and correcting problems that may occur
during operation of your analyzer.
 This chapter is not a complete service manual and is limited to problems

that are readily diagnosed and/or corrected by the user of the analyzer. If the
recommended solution fails to solve the problem, contact Mindray or your
local distributor.
 Running samples in presence of error messages may lead to misleading

results. If any error message occurs during sample analysis, remove the
error first and then re-run the sample.
 Unless otherwise instructed, always turn off the power before trying to fix
the error.

11-1
Troubleshooting Your Analyzer
11.2 Errors without available error messages
Error Possible cause(s) Recommend action

The analyzer 1. power cord is broken or not 1. check the power cord connection;
cannot be well connected; 2. Check the fuse;
turned on.
2. The fuse is broken; 3. Check the electrical outlet.
3. The power outlet has no
electricity.
Liquid drips Damaged pump hose or blocked 1. Turn off the power and wipe the
from analyzer filter. analyzer dry;
inside. 2. Call Mindray customer service

department or your local distributor
for assistance.
Recorder does 1. Recorder paper is jammed; 1. Remove the jammed paper;

not work. 2. Something is wrong with the 2. If the problem remains, turn off the
circuit. analyzer and turn it on again in 10
seconds.
11-2
11.3 Errors indicated by error messages
The analyzer can provide error messages. See the tables below for the error messages and
their probable causes and recommended action. If the problem still remains after you have
tried the recommended solutions, contact Mindray customer service department or your local
distributor.
Error Message Possible Cause(s) Recommended Action

Ambient temp. Abnormal ambient 1. Enter the “Service →Status” screen to
abnormal temperature or temperature check the ambient temperature;
transducer error.
2. If the actual ambient exceeds 15℃ to
30℃, adjust the temperature.
Otherwise, the analysis results may be
unreliable;
3. If the actual temperature is within the

pre-defined range and the problem
remains, contact Mindray customer
service department or your distributor.
Blank 1. Contaminated diluent, 1. Check if the diluent is contaminated or

abnormal diluent lines or bath; expired;
2. Expired diluent. 2. Enter the “Count” screen and press

the aspirate key to do the startup
procedure;
3. If the problem remains, enter the

“Service → Maintenance” screen and
do the probe cleanser cleaning
procedure as instructed in Chapter
10.3.6. When the procedure is
finished, return to the “Count” screen
and do the background check again;
4. If the problem remains, contact

Mindray customer service department
or your local distributor.
HGB error HGB blank voltage within 0 V 1. Do the “Probe Cleanser Cleaning”
to 3.2 V or 4.9 V to 5 V. procedure as instructed in Chapter
10.3.6.;
2. If the problem remains, adjust the

HGB gain as instructed by Chapter
11-3
5.3.4 to set the voltage within 3.4 to

4.8V, preferably 4.5V;
3. If the problem remains, shut down

your analyzer and contact Mindray
customer service department or you
local distributor.
HGB adjust HGB blank voltage within 3.2 1. Do the probe cleanser cleaning
V to 3.4 V or 4.8 V to 4.9 V. procedure as instructed in Chapter
10.3.6.;

HGB gain as instructed by Chapter
5.3.4 to set the voltage within 3.4 to
4.8V, preferably 4.5V;
3. If the problem remains, shut down

your analyzer and contact Mindray
customer service department or you
local distributor.
WBC clog 1. Clogged WBC aperture; 1. Enter the “Service → Maintenance”
2. Inappropriate WBC count screen. Zap and flush the aperture as
time setting; instructed by Chapter 10.3.4 and

10.3.5;
3. Solenoid valve error.
2. Enter the “Setup → Settings →
Count” screen and record the WBC
count time. Then enter the “Service →
Self-test” screen and test the actual
WBC count time as instructed by
Chapter 10.5.1.;
3. If the difference between the reference

WBC count time and the actual WBC
count time is less than 2 seconds, the
error has been removed;
4. If not, enter the “Service →

Maintenance” screen and do the
probe cleanser cleaning procedure as
instructed by Chapter 10.3.6;

Count” screen and record the WBC
Self Test” screen and test the actual
Chapter 10.5.1;
11-4

WBC count time and the actual WBC
7. If the difference is still greater than 2

seconds but consistent, enter the
“Setup → Settings → Count” and
reset the WBC count time. Then enter
the “Service → Self-test” screen and
test the actual WBC count time as
instructed by Chapter 10.5.1 to
confirm the difference is less than 2
seconds;

WBC bubbles 1. Diluent or rinse running 1. Check if the diluent or rinse has run
out; out. If so, change a new container of
2. Loose tube connections; diluent or rinse as instructed in

Chapter 4.4.1;
3. Inappropriate WBC
count time setting. 2. Check the connection of the diluent
and rinse pickup tube. If necessary,
reconnect and tighten them as

Chapter 5.3.5;

RBC clog 1. Clogged aperture; 1. Enter the “Service → Maintenance”
2. Inappropriate RBC screen. Zap and flush the aperture as
count time setting; instructed by Chapter 10.3.4. and

10.3.5.;
3. Solenoid valve error.
Count” screen and record the RBC
RBC count time as instructed by
Chapter 10.5.1.;
11-5
RBC count time and the actual RBC

4. If not, enter the “Service →

Maintenance” screen and do the
probe cleanser cleaning procedure as
instructed by Chapter 10.3.6.;

Count” screen and record the RBC
RBC count time as instructed by
Chapter 10.5.1.;

RBC count time and the actual RBC
7. If the difference is still greater than 2

seconds but consistent, enter the
“Setup → Settings → Count” and
reset the RBC count time. Then enter
the “Service → Self-test” screen and
test the actual RBC count time as
instructed by Chapter 10.5.1 to
confirm the difference is less than 2
seconds;

RBC bubbles 1. Diluent or rinse running 1. Check if the diluent or rinse has run
out; out. If so, change a new container of
2. Loose tube connections; diluent or rinse as instructed in

Chapter 4.4.1;
3. Inappropriate RBC
count time setting. 2. Check the connection of the diluent
and rinse pickup tube. If necessary,
reconnect and tighten them as
3. If the problem remains, adjust the RBC

count time as instructed by Chapter
5.3.5;
11-6

Trans error 1. Communication cable not 1. Check if the communication cable is

well connected; well connected;
2. Inappropriate 2. Check the communication settings as

communication settings. instructed by Chapter 5.3.2 and make
sure they are the same with the host.
Barcode error 1. Poor connection between 1. Check if the analyzer is well connected
the scanner and the to the analyzer;
analyzer; 2. Check if the bar-code is valid;
2. Invalid bar-code. 3. If the problem remains, contact
Barcode com Poor connection between the 1. Check if the analyzer is well connected
scanner and the analyzer. to the analyzer;
error
Printer out of Printer paper running out or 1. Check if there is printer paper;
not properly installed.
paper 2. Check if the printer paper is well
installed.
Printer offline Poor connection between the Check if the printer is well connected to the
printer and the analyzer. analyzer.
Recorder com 1. Poor connection between 1. Print again;
the recorder and the 2. If the problem remains, shut down the
error
analyzer; analyzer and restart it again, and then
print again;
2. Damaged recorder.
3. If the problem remains, shut down the
analyzer and contact Mindray
customer service department.
Recorder out of Recorder paper running out 1. Check if the recorder paper has run
or not properly installed. out. If so, install the paper as
paper
2. Check if the recorder paper is properly

installed. If not, re-install the paper as

11-7
Recorder too hot Recorder head too hot. 1. Stop using recorder and cool it for 10
minutes and then print again;
analyzer and restart it again, and then
print again;
Mindray customer service department.
Lyse out Insufficient lyse or wrong lyse 1. Check if there is sufficient lyse left. If
volume setting. so, access “Setup → Settings →
Reagents” and adjust the remaining
lyse volume as instructed by Chapter
5.3.1;
2. If not, change a new container of lyse

as instructed by Chapter 4.4.1.
Diluent expired Expired diluent or wrong 1. Check if the diluent has expired. If so,
expiration setting change a new container of diluent as
2. If not, reset the expiration date as

instructed in Chapter 5.3.1.
Rinse expired Expired rinse or wrong 1. Check if the rinse has expired. If so,
expiration setting change a new container of rinse as

Lyse expired Expired lyse or wrong 1. Check if the lyse has expired. If so,
expiration setting change a new container of lyse as

Vacuum filter The air inside the vacuum 1. Enter “Service → Self–test →
chamber is not extracted Tubing”to test the filter as instructed
error
within the given time. in Chapter 10.5.1;
2. If the test result is normal, the error will

be removed;
3. If the problem remains, replace the

filter as instructed by Chapter 10.11;
4. If the problem still remains after a new

filter has been installed, contact
11-8
Real-time clock 1. Someone tempered with 1. Enter “Setup → Settings → Date &
error the on-board battery off Time” screen and reset the time as
the board; instructed by Chapter 5.3.3;
2. Something is wrong with 2. Restart the analyzer after the

the on-board battery adjustment and the time should be
(poor contact, dead correct;
battery, etc.); 3. If the problem remains, contact
3. Damaged real-clock chip. Mindray customer service department
Syringe motor 1. Poor contact of the 1. Enter “Service → Self-test → Machine”

error motor; to test the syringe motor as instructed
2. Damaged motor; by Chapter 10.5.2.;
3. Poor connection between 2. If the test result is normal, the error will
the power drive board be removed;
and the CPU board; 3. If the problem remains, contact Mindray
4. Malfunctioning customer service department or your
photocoupler. local distributor.
Rotation Motor 1. Jammed sample probe; 1. Open the front door and check if the
Error 2. Poor contact of the signal sample probe is jammed;
line; 2. Enter “Service → Self Test →
3. Damaged motor; Machine”screen to test the rotation

motor as instructed by Chapter
4. Poor connection between
10.5.2.. If the test result is normal, the
the drive board and the
error will be removed;
CUP board;
5. Malfunctioning photo
coupler.
Elevator motor 1. Jammed sample probe; 1. Open the front door and check if the
error 2. Poor contact of the signal sample probe is jammed;
line; 2. Enter the “Service → System Test”
3. Damaged motor; screen and check the motor as

4. Poor connection between
the drive board and the 3. The error will be removed if the test
CUP board; result is normal;
5. Malfunctioning photo 4. If the problem remains, contact
coupler. Mindray customer service department

11-9
A/D error Something is wrong with the 1. Enter the “Service → Self-test →
A/D part of the CPU board. Circuit” screen to test the A/D
interrupt as instructed by Chapter
10.5.4.;

be removed;

analyzer and contact Mindray
customer service department or your
local distributor.
Vacuum error The vacuum degree does not 1. Check whether the external tubing is
reach the expected value pressed;
within the given time.
2. If not, enter “Service→ Self-test →
Tubing” to check the vacuum as
instructed by Chapter 10.5.1..;

be removed;

Pressure error The pressure inside the 1. Check whether the external tubing is
pressure chamber does not pressed;
reach the expected value
2. If not, enter “Service→ Self-test →
within the given time
Tubing”screen to check the pressure
as instructed by Chapter 10.5.1..;

be removed.

Diluent empty Insufficient diluent or wrong 1. Check if there is sufficient diluent left. If
diluent volume setting. so, access “Setup → Settings →
diluent volume as instructed by
Chapter 5.3.1;
2. If not, change a new container of

diluent as instructed by Chapter 4.4.1.
Rinse empty Insufficient rinse or wrong 1. Check if there is sufficient rinse left. If
rinse volume setting. so, access “Setup → Settings →
11-10

diluent volume as instructed by
Chapter 5.3.1;
2. If not, change a new container of rinse

as instructed by Chapter 4.4.1.
Waste full The waste container is full. Empty the waste container and reset
usable volume of the waste container as
instructed by Chapter 5.3.1.
File error Something is wrong with the Turn off the power and contact Mindray
analyzer’s file system. customer service department or your local
distributor.
Dynamic Something is wrong with the Turn off the power and contact Mindray
analyzer’s memory. customer service department or your local
memory error
distributor.
Analog power Something is wrong with the Shut down the analyzer and contact
power board. Mindraycustomer service department or
error
your local distributor.
11-11
12 Appendices
A Index
WBC, 11-4
control, 2-16
A Impedance Principle, 3-1
analyzer count
Intended use, 2-2 principle, 3-1
name, 2-1 procedure, 3-5
aperture screen, 2-11
flush, 10-7 customize
zap, 10-6 date & time, 5-11
aperture size, 12-4 gain, 5-13
aspirate key, 2-7 other, 5-21
aspiration, 3-2 Print & comm., 5-7
reagent, 5-5
reference range, 5-19
B CV
bath definition, 3-10
clean, 10-15 formula, 8-11
drain, 10-16
baud rate, 5-8
Blank Photocurrent, 3-6
D
bubbles DB9 connector, 12-16
RBC, 11-7 diluent
WBC, 11-5 connection, 4-5
definition, 2-15
prime, 10-3
C dilution, 3-3
calibration dimensions, 3-5
auto, 9-4 drain tubing, 10-18
calibrators, 2-16
manual, 9-10
preparations, 9-3
E
procedures, 9-3 environment, 4-3
purpose, 9-1 error
cleanser A/D error, 11-10
use, 10-11 ambient temp. abnormal, 11-3
clog barcode com error, 11-7
RBC, 11-6 barcode error, 11-7
A-1
Appendices
blank abnormal, 11-4 HCT

diluent empty, 11-11 definition, 3-10
diluent expired, 11-9 formula, 3-10
dynamic memory error, 11-12 HGB
elevator motor error, 11-10 carryover, B-3
filter error, 11-11 definition, 3-5
HGB adjust, 11-4 linearity range, B-3
HGB error, 11-4 operating range, B-3
lyse expired, 11-9 humidity, B-5
lyse out, 11-8
press bar up, 11-8
pressure error, 11-11
I
printer offline, 11-8 ID, 6-10
printer out of paper, 11-8 installation
real-time clock error, 11-9 requirement, 4-2
recorder com error, 11-8
recorder out of paper, 11-8
recorder too hot, 11-8
L
rinse empty, 11-11 LCD, 2-7
rinse expired, 11-9 leukocyte
rotation motor error, 11-10 granulocyte, 2-2
syringe motor error, 11-9 lymphocyte, 2-2
trans error, 11-7 monocyte, 2-2
vacuum error, 11-10 Lymph#
vacuum filter error, 11-9 definition, 3-7
waste full, 11-11 formula, 3-7
E-Z cleanser Lymph%
definition, 2-15 definition, 3-6
formula, 3-6
lyse
G connection, 4-7
gain definition, 2-15
set HGB gain, 5-14 Lyse test, 10-13
set RBC gain, 5-13
Gran#
definition, 3-7
M
formula, 3-7 maintenance
Gran% system, 10-3
definition, 3-6 MCH
formula, 3-6 definition, 3-10
formula, 3-10
MCHC
H definition, 3-10
handshake, 5-9 formula, 3-10
A-2
Appendices
MCV prepare to ship, 10-21

definition, 3-9 printer
linearity range, B-3 connection, 4-10
operating range, B-3 format, 5-7
Mon# probe cleanser
definition, 3-7 use, 10-8
formula, 3-7 probe cleaser
Mon% definition, 2-15
definition, 3-6 probe wipe
formula, 3-6 clean, 10-19
MPV replace, 10-36
definition, 3-10 programming, D-4
N Q
NRBC, 3-6 QC
edit settings, 8-2
L-J graph, 8-10
O run, 8-6
optical sensors, 3-4 table, 8-12
P R
parameter description, 2-3 RBC
password, 5-2 definition, 3-9
PCT linearity range, 12-5
definition, 3-11 measurement, 3-9
formula, 3-11 operating range, B-3
PDW RDW, 3-10
definition, 3-10 reagent
performance specifications connection, 4-5
linearity range, B-3 recorder
operating range, B-3 format, 5-8
PLT reference range, 5-19
definition, 3-10 rinse
linearity range, B-3 connection, 4-6
operating range, B-3 definition, 2-15
power prime, 10-5
fuse, 4-2 RS-232 serial port, D-2
voltage, 4-2
predilute mode
sample collection and handling, 6-9
S
prediluted mode sample
analyze, 6-17 analysis, 6-11
A-3
Appendices
review, 7-1
sample probe
U
adjust, 10-32 unpacking, 4-4
sample probe localizer, 10-32
shutdown, 6-24
specification, B-3
V
system valve
self-test, 10-24 test, 10-26
status, 10-23
W
T WBC
table definition, 3-6
sample, 7-4 formula, 3-6
searched, 7-11 linearity range, B-3
throughput, B-3 operating range, B-3
transmission weight, B-6
at QC table screen, 8-13
at review screen, 7-18
data format, D-4
Z
troubleshooting, 11-39 zap aperture, 10-6
A-4
B Specifications
B.1 Reagents
Diluent V-28D DILUENT
Rinse V-28R RINSE
Lyse V-28CFL LYSE
E-Z Cleanser（Enzyme cleanser） V-28E E-Z CLEANSER
Probe Cleanser V-28P PROBE CLEANSER
Calibrator Specified by Mindray
Control Specified by Mindray
B.2 Parameters
Table B-1 Directly measured parameters and histograms
Parameter Abbreviation Default
9
White Blood Cell or leukocyte WBC 10 /L
Red Blood Cell or erythrocyte RBC 1012/L
Hemoglobin Concentration HGB g/L
Platelet PLT 109/L
WBC histogram WBC Histogram /
RBC histogram RBC Histogram /
PLT histogram PLT Histogram /
Table B-2 Parameters derived from histograms

Lymphocyte percentage Lymph% %
Monocyte percentage Mon% %
Granulocyte percentage Gran% %
Eosinophils percentage Eos% %
Mean Corpuscular Volume MCV fL
Red Blood Cell Distribution Width RDW %
Mean Platelet Volume MPV fL
Platelet Distribution Width PDW /
Table B-3 Calculated parameters

9
Lymphocyte Lymph# 10 /L
Monocyte Mon# 109/L
B-1
Appendices
Granulocyte Gran# 109/L

Hematocrit HCT %
Mean Cell Hemoglobin MCH pg
Mean Cell Hemoglobin Concentration MCHC g/L
Mean Platelet Volume PCT %
Table B-4 Parameters and animals

Parameter Dog Cat Horse Rat Mouse Rabbit Monkey Cow Pig Buffalo Sheep Camel Goat User-
defined
WBC √ √ √ √ √ √ √ √ √ √ √ √ √ √
Lymph# √ √ √ √ √ √ √ √
Mon# √ √ √ √ √ √ √ √
Gran# √ √ √ √ √ √ √ √
Lymph% √ √ √ √ √ √ √ √
Mon% √ √ √ √ √ √ √ √
Gran% √ √ √ √ √ √ √ √
RBC √ √ √ √ √ √ √ √ √ √ √ √ √ √
HGB √ √ √ √ √ √ √ √ √ √ √ √ √ √
MCV √ √ √ √ √ √ √ √ √ √ √ √ √ √
MCH √ √ √ √ √ √ √ √ √ √ √ √ √ √
MCHC √ √ √ √ √ √ √ √ √ √ √ √ √ √
RDW √ √ √ √ √ √ √ √ √ √ √ √ √ √
HCT √ √ √ √ √ √ √ √ √ √ √ √ √ √
PLT √ √ √ √ √ √ √ √ √ √ √ √
MPV √ √ √ √ √ √ √ √ √ √ √ √
PDW √ √ √ √ √ √ √ √ √ √ √ √
PCT √ √ √ √ √ √ √ √ √ √ √ √
Eos% √ √
WBC √ √ √ √ √ √ √ √ √ √ √ √ √ √
Histogram
RBC √ √ √ √ √ √ √ √ √ √ √ √ √ √
Histogram
PLT √ √ √ √ √ √ √ √ √ √ √ √
Histogram
B.3 Sampling Features
B.3.1 Sample volumes required for each analysis

Whole Blood Mode (vein blood) 13 μL
Prediluted Mode (capillary blood) 20 μL
B-2
Appendices
B.3.2 Aperture size
Diameter Length
Aperture 80 μm 70 μm
B.3.3 Throughput
Less than 150 seconds/analysis
B.4 Performance specifications
B.4.1 Operating range
Parameter Operating range

9
WBC (10 /L) 0.0 to 499.9
12
RBC (10 /L) 0.00 to 29.99
HGB (g/L) 0.0 to 350.0
MCV (fL) 0.0 to 250.0
9
PLT (10 /L) 0 to 3999
B.4.2 Normal background
Parameter Background result

WBC ≤ 0.3  109 / L
RBC ≤ 0.03 1012/ L
HGB ≤1g/L
HCT ≤ 0.5 %
PLT ≤ 10  109 / L
B.4.3 Linearity range
Parameter Linearity range

9
WBC (10 /L) 0.4 to 99.9
12
RBC (10 /L) 0.2 to 16.99
HGB (g/L) 3 to 250
9
PLT (10 /L) 10 to 2999
B-3
Appendices
B.4.4 Carryover
Parameter Carryover
WBC ≤ 0.5 %
RBC ≤ 0.5 %
HGB ≤ 0.5 %
PLT ≤1%
B.4.5 Reproducibility (with normal-level control)
Parameter Condition Reproducibility（CV%）

WBC 7.5 to 15.0  109 / L ≤ 3.0
RBC 3.50 to 6.00  1012 / L ≤ 2.0
HGB 120 to 180 g/L ≤ 2.0
MCV 40.0 to 110.0 fL ≤ 2.0
PLT 200 to 500  109 / L (MCV>60.0) ≤ 6.0
B.5 Input/Output Device
 Use the specified devices only.
B.5.1 Keypad
18-key keypad.
B.5.2 Keyboard
PS/2 keyboard.
B.5.3 Bar-code scanner (optional)
B.5.4 Display
Color LCD, 7.8″ 640×480
B.5.5 Recorder
Built-in thermal recorder that supports two printing formats and auto printing
B-4
Appendices
B.5.6 Recorder paper
Width: 50 00.7 mm
 To ensure the print quality and operating life of the recorder, it is

recommended that you purchase the recorder paper from the analyzer
manufacturer.
B.5.7 Printer（optional）
EPSON LX-300+.
B.5.8 Interfaces
 A keyboard interface.
 Two RS-232 interfaces (maximum transmission distance 12 meters);
 A parallel port(for printer or floppy disk drive);
 A power supply for the floppy disk drive（only to be used with the power cable
supplied by Mindray）.
 IDE hard disk interface.
B.6 Power supply

 Voltage: 100 to 240 VAC;
 Frequency: 50/60 Hz;
 Input power:180 VA;
 Fuse: 250 V T4A
 Use the fuse of the specified type and rating.
B.7 EMC Description

 This equipment complies with the emission and immunity requirements of the EN
61326-1:2006 and EN 61326-2-6:2006.
B-5
Appendices
 This equipment has been designed and tested to CISPR 11 Class A. In a domestic
environment it may cause radio interference, in which case, you may need to take
measures to mitigate the interference.
B.8 Sound
Maximal sound: 65 dB
B.9 Operating environment

 Operating temperature:15 ℃ to 30 ℃;
 Relative humidity: 30 % to 85 %;
 Atmospheric pressure: 60 kPa to 106 kPa.
B.10 Storage environment

 Ambient temperature: －10 ℃ to 40 ℃
 Relative humidity: 10 % to 93 %
 Atmospheric pressure: 50 kPa to 106kPa
B.11 Dimensions
Depth Width Height
38.6 cm 32.2 cm 43.7 cm
B.12 Weight
Less than 23 kg
B.13 Contraindications
None.
B-6
C Precautions, Limitations and Hazards
C.1 Introduction
You will find the following symbols in this manual.
When you see… Then…

Read the statement below the symbol. The statement is
alerting you to an operating hazard that can cause
personnel injury.
alerting you to a possibility of analyzer damage or unreliable
analysis results.
alerting you to information that requires your attention.

alerting you to a potentially biohazardous condition.
C.1.1 Installation Requirements

All the space, power and environmental requirements listed in Chapter 4 and Appendix B
must be met. Establishing and maintaining proper grounding cannot be overemphasized.
C.1.2 Limitations
Whenever the results are outside the normal limits, it is recommended that the laboratory
following whatever written protocol is in place for validating results.
If an error occurs, the analyzer displays the corresponding error message In case of errors
related to the fluidic system (such as clogging or bubbles), it is recommended that you re-run
the sample after removing the error.
If the PLT value is less than 100  109 / L, it is recommended the result be verified by a
microscope.
C.1.3 Maintenance
The maintenance instructions in Chapter 10 describe corrective and preventive procedures
that must be followed to ensure proper operation and performance of your analyzer.
C-1
Appendices
C.2 Warnings
 It is important for the hospital or organization that employs this equipment

to carry out a reasonable service/maintenance plan. Neglect of this may
result in machine breakdown or injury to human health.
 Operate the analyzer under the condition specified in this manual;

otherwise, the analyzer will not work normally and the analysis results will
be unreliable, which would damage the analyzer components and cause
personal injury.
 Make sure the analyzer is properly grounded.
 Before turning on the analyzer, make sure the input voltage meets the above
requirements.
 When moving the analyzer, face the front of the analyzer and carry it from
the bottom with hands!
doctor.
 Do not place the analyzer in a flammable or explosive environment.

 Avoid direct contact with animal blood samples.
 To avoid personal injury, keep your clothes, hair and hands away from such
moving parts as the sample probe.
 Only install a fuse of the specified type and rating.
 Unless otherwise instructed, always turn off the power before trying to fix
the error.
 Wear proper personal protective equipment (e.g. medical gloves, disposable

wrist strap, etc.) against the static electricity during maintaining recorder.
 Make sure the power supply of the analyzer has been cut off before
maintenance.
C-2
Appendices
C.3 Cautions
 Installation by personnel not authorized or trained by Mindray may damage

your analyzer. Do not install your analyzer without the presence of
Mindray-authorized personnel.
 Do not place any container on the top of the analyzer.
 Do not re-use disposable products.
 Do not perform any maintenance procedures that are not described in this
chapter. Performing unauthorized maintenance procedures can damage
your analyzer.
 In case of problems not specified in this manual, contact Mindray customer

service department or your local distributor for assistance.
 Only Mindray-supplied parts can be used for maintenance. For any

questions, contact Mindray customer service department or your local
distributor.
 Use only specified recorder paper. Otherwise, it may cause damage to the
recorder head, or the recorder may be unable to print, or poor print quality
may result.
 Never pull the recorder paper with force when a recording is in process.
Otherwise, it may cause damage to the recorder.
 Do not leave the recorder door open unless you install paper or remove
trouble.
 Improper installation of recorder paper may jam the paper and/or result in
blank printout.
 If the recorder finishes a print action, wait at least 20 minutes until the
recorder head is cooled completely and then maintain the recorder.
 Do not add unnecessary force to the recorder head.
 Wipe off the alcohol remaining on the recorder head in time after cleaning
the recorder head.
 Make sure the alcohol volatilizes completely before using the recorder to
print.
C-3
Appendices
C.4 Notes
 This equipment must be operated by skilled/trained medical professionals.
 Operate your analyzer strictly as instructed in this manual.
 This analyzer adopts a fixed decimal point. You can enter the digits without
bothering to look for the [.] on the external keyboard.
 The purpose of this analyzer is to identify the normal animal patient, with all
normal system-generated parameters, and to flag or identify patient results
that require additional studies.
 Before connecting the power cord, make sure the power switch at the back
of the analyzer is placed in the off (O) position.

solutions.
 Retain the shipping carton and all the packing materials, as they can be
used for packaging if analyzer must be reshipped.
 Use the specified reagents.
 Never use expired reagents.
 To prevent contamination, tighten the container caps when the installation is

finished.
 Store and use the reagents as directed by instructions for use of the
reagents.
 When you have changed the diluent, rinse or lyse, run a background to see
if the results meet the requirement.
 Pay attention to the expiration dates and open-container stability days of all
the reagents. Never use expired reagents.
 The recorder paper is treated on one side for printing. To determine which
side is the printing side, gently scratch both sides with a fingernail and the
one with visible nail trace left is the printing side.
 For the external printer, you can only select “Vertical”.
 Once you have selected another animal, the analysis result and sample
information regarding the current sample will be cleared, but the next
C-4
Appendices
sample ID will remain unchanged.
 Whole blood samples to be analyzed for WBC differential or PLT count

should be stored at room temperature and run within 4 hours of collection.
 If you do not need the PLT, MCV and WBC differential results, you can store
the samples in a refrigerator (2℃ to 8℃) for 24 hours. You need to warm the
refrigerated samples at room temperature for at least 30 minutes before
running them.
 After mixing the capillary sample with the diluent, wait 5 minutes before
running the sample.
 Run the prediluted samples within 30 minutes after the mixing.



the corresponding error messages will be displayed in the error message
area and the results of all the related parameters will be invalidated. See

 To ensure stable analyzer performance and accurate analysis results,

perform the “Shutdown” procedure to shut down the analyzer after it has
been running continuously for 24 hours.
 Shut down the analyzer strictly as instructed below.
 Fur and skin debris may block the aperture. Keep the sample clean before
using the analyzer to analyze it.
 Samples of the dogs infected with Filaria Larva in Blood may clog the
aperture.
 For every search, the analyzer can display a maximum of 500 matches.
 The matches will be deleted if you have run another sample (including
background check), or deleted a sample result, or restarted the analyzer
C-5
Appendices
after the search.
 Use the specified controls. Using controls other than the specified will lead
to misleading results.
 Refer to the instructions of use of the controls for how to store and use the
controls.
 Refer to the instructions of use of the control for information on the lot
number, expiration date, open-vial stability days, expected results and limits.
 The entered expiration date should be either the expiration date printed on
the labeling or the open-vial expiration date. It is earlier.
 The open-vial expiration date is calculated as follows: the date that vial is
opened + the open-vial stability days.
 At the “QC Edit” screen, if you want to correct an erroneous entry, MODIFY
the wrong digit.
 All of the measured parameters must be calibrated before readings of this

analyzer can be used as valid analysis results.
 Use the Mindray- specified calibrator. Using calibrator other than the
specified will lead to misleading results.
 Refer to the instructions of use of the calibrator for how to store and use the
calibrator.
 In the prediluted “Calibration” screen, you cannot dispense diluent from the
analyzer. It is recommended that you prepare at least 7 cups of diuent before
starting calibrating the analyzer in the prediluted mode.
 Do the “Drain Tubing” procedure before relocating the analyzer.
 This chapter is not a complete service manual and is limited to problems

that are readily diagnosed and/or corrected by the user of the analyzer. If the
recommended solution fails to solve the problem, contact Mindray or your
local distributor.
 Remove the protective paper between the recorder head and the roller
inside the recorder before installing recorder paper.
 Maintain the recorder strictly as instructed below.
 To ensure the print quality and operating life of the recorder, it is

recommended that you purchase the recorder paper from the analyzer
manufacturer.
C-6
Appendices
C.5 Biohazard

C-7
D Communication
D.1 Introduction
The BC-2800Vet can transmit the sample data and QC data to an external computer (a host)
through its RS-232 serial port. The transmission can be conducted either automatically or
through the command of the operator after the completion of the sample analysis. This
section gives detailed discussion about the setup of transmission parameter, RS-232 serial
port and the data transmission format, therefore, providing detailed information for the
software engineers to program and for the user to conveniently perform transmission.
D-1
Appendices
D.2 Connection
The BC-2800Vet can be connected with an external computer through a DB9 connector. The
pins of the DB9 connector are shown in Figure D-1.
Figure D-1 DB9 connecter
Pin description:
DCD: Carrier Detect
RXD: Receive Data
TXD: Transmit Data
DTR: Data Terminal Ready
GND: Signal Ground
DSR: Data Set Ready
RTS: Request to Send
CTS: Clear to Send
RI: Ring Indicator
The BC-2800Vet communicates with a host through serial port 2, using Pin2, Pin 3 and Pin 5.
The maximum transmission distance is 12 meters.
D-2
Appendices
D.3 Transmission Data Format
D.3.1 Description
Symbols
[ENQ] 0x05
[STX] 0x02
[EOT] 0x04
[EOF] 0x1A
[ETX] 0x03
[ACK] 0x06
[NACK] 0x15
"A" 0x41
"B" 0x42
"C" 0x43
"#" 0x30-0x39
"*" 0x2A
If the Lot No., Month, Day, Year are empty in QC Edit menu, the “*” (2A Hex) will be
transmitted to the host.
For all the data formats, if the data are marked “*”, then “*” (2A Hex) will be transmitted to the
host.
L1 Region to L8 Region are LI to L8 of eight histogram discriminators as shown in Figure D-2.
Figure D-2 L1 to L8
D-3
Appendices
Programming
If the Handshake is off, the BC-2800Vet will transmit the body of the text without
acknowledging the presence of an external computer.
If the Handshake is on, the BC-2800Vet will communicate with the external computer in
following procedures:
1. The BC-2800Vet sends an ENQ (05 Hex), then waits up to 4 seconds for the external
computer to respond. If the external computer does not respond, then one more ENQ (05
Hex) is tried. If it fails again, the analyzer aborts the transmission and reports a
transmission error;
2. The external computer must respond by sending an ACK (06 Hex). If any other response
is received, another ENQ (05 Hex) will be sent by the analyzer (a maximum of two ENQ
[05 Hex] will be sent);
3. The analyzer then sends:
Body of text
EOT (04 Hex)
ETX (03 Hex)
4. Disconnection.
The BC-2800Vet sends an ETX (03 Hex), then waits 4 seconds for the external computer
to respond. If no response is received, one more ETX ( 03 Hex) is sent, the BC-2800Vet
waits 4 seconds before giving up and gives alarm of communication error.
If the external compute responds ACK, the transmission is done successfully. If the external
computer responds NACK（15 Hex）, the analyzer repeat the transmission from step 3. If the
received response from the computer is neither ACK（06 Hex） nor NACK（15 Hex）, the
analyzer sends ETX ( 03 Hex) again.
D.3.2 Sample Data Format
Animal Type:
00 Dog
01 Cat
02 Horse
03 Pig
04 Cow
05 Buffalo
06 Goat
D-4
Appendices
07 Animal1
08 Animal2
09 Animal3
11 Rat
12 Mouse
13 Rabbit
14 Camel
15 Sheep
16 Monkey
For Cat, Dog, Horse, Rat, Mouse, Rabbit, Monkey and Cow
If handshake is enabled [ENQ]

If handshake is disabled [STX]
Body of the text start
Text Identifier “A”
ID ########
Sample Mode #
Month ##
Day ##
Year ####
Hour ##
Minutes ##
9
WBC[10 /L] ###.#
9
Lymph#[10 /L] ###.#
9
Mon#[10 /L] ###.#
9
Gran#[10 /L] ###.#
Lymph%[%] ##.#
Mon%[%] ##.#
Gran%[%] ##.#
12
RBC[10 /L] ##.##
HGB[g/L] ###
MCHC[g/L] ####
MCV[fL] ###.#
MCH [pg] ###.#
RDW [%] ##.#
HCT[%] ##.#
9
PLT[10 /L] ####
MPV[fL] ##.#
PDW ##.#
PCT[%] .###
Reserved ####
Eos% (Reserved if not ###
D-5
Appendices
available)
Reserved #######
AnimalType ##
Reserved ########
L1 Region ###
L2 Region ###
L3 Region ###
L4 Region ###
L5 Region ###
L6 Region ###
L7 Region ###
L8 Region ###
Reserved ################
WBC Histo (256 channels) ###
RBC Histo (256 channels) ###
PLT Histo (256 channels) ###
Body of the text end
If handshake is enabled [EOT]
If handshake is disabled [EOF]
If handshake is enabled [ETX]
For pig, Buffalo, Camel, Sheep and User-defined

ID ########
Sample Mode #
Month ##
Day ##
Year ####
Hour ##
Minutes ##
9
WBC[10 /L] ###.#
Reserved ################
Reserved #####
12
RBC[10 /L] ##.##
HGB[g/L] ###
MCHC[g/L] ####
MCV[fL] ###.#
MCH [pg] ###.#
RDW [%] ##.#
D-6
Appendices
HCT[%] ##.#
9
PLT[10 /L] ####
MPV[fL] ##.#
PDW ##.#
PCT[%] .###
Reserved ##############
AnimalType ##
Reserved ########
L1 Region ###
L2 Region ###
L3 Region ###
L4 Region ###
L5 Region ###
L6 Region ###
L7 Region ###
L8 Region ###
Reserved ################
For Goat

ID ########
Sample Mode #
Month ##
Day ##
Year ####
Hour ##
Minutes ##
9
WBC[10 /L] ###.#
Reserved ################
Reserved #####
12
RBC[10 /L] ##.##
HGB[g/L] ###
D-7
Appendices
MCHC[g/L] ####
MCV[fL] ###.#
MCH [pg] ###.#
RDW [%] ##.##
HCT[%] ##.#
Reserved #############
Reserved ##############
Animal Type ##
Reserved ########
L1 Region ###
L2 Region ###
L3 Region ###
L4 Region ###
L5 Region ###
L6 Region ###
L7 Region ###
L8 Region ###
Reserved ################
D.3.3 Standard L-J QC Data Format

Text Identifier “B”
File No. #
Lot No. ######
Month ##
Day ##
Year ####
9
WBC[10 /L] ###.#
12
RBC[10 /L] ##.##
HGB[g/L] ###
9
PLT[10 /L] ####
Reserved ##############
D-8
Appendices
HCT[%] ##.#
MCV[fL] ###.#
MCH[pg] ###.#
MCHC[g/L] ####
9
WBC Limit[10 /L] ###.#
12
RBC Limit[10 /L] ##.##
HGB Limit[g/L] ###
9
PLT Limit[10 /L] ####
Reserved ##############
HCT Limit[%] ##.#
MCV Limit[fL] ###.#
MCH Limit[pg] ###.#
MCHC Limit[g/L] ####
D.3.4 Run L-J QC Data Format

Text Identifier ‘C’
Month ##
Day ##
Year ####
Hour ##
Minutes ##
9
WBC[10 /L] ###.#
12
RBC[10 /L] ##.##
HGB[g/L] ###
9
PLT[10 /L] ####
Reserved ##############
HCT[%] ##.#
MCV[fL] ###.#
MCH[pg] ###.#
MCHC[g/L] ####
D-9
Appendices
D.4 Transmission
D.4.1 Defining Transmission Settings

The data format is fixed for the transmission so that every byte to be transmitted has 7 data
bits and 1 stop bit. Enter the ”Setup → Settings→ Print & comm.” screen and edit the
communication settings as instructed by Chapter 5.3.2.
D.4.2 Transmission at “Count” Screen

If the auto transmission function is on, once the analysis is done, the analyzer will
automatically transmit the results to the external computer. If the auto transmission function is
off, you can only transmit the results manually at the “Review “screen.
D.4.3 Transmission at the “Review” Screen

Select the results you want to transmit and transmit them to the external computer as
instructed by Chapter 7.2.2. and Chapter 7.3.2.
D.4.4 Transmission at the “QC Table” Screen

Transmit the results as instructed by Chapter 8.4.2..
D-10
P/N: 2810-20-47202（12.0）

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BC-2800Vet Auto Hematology Analyzer

For this Operator’s Manual, the issued date is 2010-09.

Intellectual Property Statement

SHENZHEN MINDRAY BIO-MEDICAL ELECTRONICS CO., LTD. (hereinafter called

Mindray intends to maintain the contents of this manual as confidential information.

Release, amendment, reproduction, distribution, rental, adaptation, translation or any other

Mindray is strictly forbidden.

, , are the trademarks, registered or otherwise, of Mindray in

Responsibility on the Manufacturer Party

Contents of this manual are subject to change without prior notice.

 the product is used in accordance with the instructions for use.

to carry out a reasonable service/maintenance plan. Neglect of this may

result in machine breakdown or personal injury.

be unreliable, which would damage the analyzer components and cause

z This equipment must be operated by skilled/trained clinical professionals.

THIS WARRANTY IS EXCLUSIVE AND IS IN LIEU OF ALL OTHER WARRANTIES,

This warranty shall not extend to:

 Malfunction or damage caused by improper use or man-made failure.

 Malfunction or damage caused by unstable or out-of-range power input.

 Malfunction or damage caused by force majeure such as fire and earthquake.

 Malfunction or damage caused by improper operation or repair by unqualified or

 Others not caused by instrument or part itself.

E-mail Address: [email protected]

Tel: +86 755 26582479 26582888

Fax: +86 755 26582934 26582500

EC-Representative: Shanghai International Holding Corp. GmbH(Europe)

Address: Eiffestraβe 80, Hamburg 20537, Germany

1 Using This Manual ................................................................................... 1-1

2 Understanding Your Analyzer................................................................. 2-1

3 Understanding the System Principles ................................................... 3-1

3.4 WBC/HGB Measurement....................................................................... 3-4

4 Installing Your Analyzer .......................................................................... 4-1

5 Customizing the Analyzer Software ....................................................... 5-1

6 Operating Your Analyzer ......................................................................... 6-1

7 Reviewing Sample Results ..................................................................... 7-1

8 Using the QC Programs .......................................................................... 8-1

9 Using the Calibration Programs ............................................................. 9-1

9.1 Introduction ............................................................................................ 9-1

10 Maintaining Your Analyzer .................................................................... 10-1

11 Troubleshooting Your Analyzer ............................................................ 11-1

12 Appendices ............................................................................................ 12-1

A Index ....................................................................................................... 12-1

C Precautions, Limitations and Hazards ...................................................C-1

 Operate your analyzer strictly as instructed in this manual.

1.2 Who Should Read This Manual

This manual contains information written for clinical laboratory professionals to

 learn about the BC-2800Vet hardware and software;

 customize system settings;

 perform daily operating tasks;

 perform system maintenance and troubleshooting.

1.3 How to Find Information

If you want to … See …

1.4 Conventions Used in This Manual

1.5 Special Terms Used in This Manual

When you read … It means …

You will find the following symbols in this manual.

When you see… Then…

read the statement below the symbol . The statement is

When you see… It means…

the product is used in accordance with the instructions for use.

Malfunction or damage caused by improper use or man-made failure.

Malfunction or damage caused by unstable or out-of-range power input.

Malfunction or damage caused by force majeure such as fire and earthquake.

Malfunction or damage caused by improper operation or repair by unqualified or

Others not caused by instrument or part itself.