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For (GCMS-QP2010 Ultra / SE)

This document provides quick and easy operational guidelines for GCMSsolu-

tion Ver. 2.6. Please keep this document beside your GC/MS in order to quickly

confirm operational procedures. For more detailed operational procedures,

please refer to the "Gas Chromatograph Mass Spectrometer GCMS-QP2010

Series Operation Guide for GCMSsolution Ver. 2.6".

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1 Daily Startup and Shutdown GCMS Data Acquisition Program

Please do not shut down GC/MS system because several hours will be required /P 0QFSBUJPO %FTDSJQUJPO
for starting-up. The PC can be switched off except during analysis. The CRT can  0/ Turn the CRT ON.
be switched on and off whenever required.
 $MJDL Cancel the ecology mode.


2 Executing Autotuning GCMS Data Acquisition Program 

Set the method file you are going to use for the analysis. After the GC/MS system r
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is ready for the analysis, perform autotuning.*1*2 /P 0QFSBUJPO %FTDSJQUJPO
 $MJDL Open the [Peak Monitor] sub-window.

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$POEJUJPOT  $MJDL Create a new tuning file.

/P 0QFSBUJPO %FTDSJQUJPO  $MJDL Select the tuning mode. 
 $IFDL Check that the window is the [Acquisition] sub-window.  $MJDL Select the filament as required.
 %PVCMFDMJDL Load the method file to be used.  $MJDL Start autotuning.
 $MJDL The setup parameters are sent to the instrument.  *OQVU Enter the file name.
 $IFDL The instrument is ready if the values are the set values.  $MJDL Save the tuning file.
 $MJDL Open the [Tuning] sub-window.  $IFDL Check the report that is output.*3

*1 Execute autotuning after creating the method file if it has not already been created. *3 To check the results of autotuning, refer to the
*2 Whenever autotuning is executed, calibration curves must be newly created. Operation Guide "2.7.3 Checking Autotuning Results".


 



 

 


 

 
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3 Creating Folders GCMS Data Acquisition Program

Files with file types in the figure on the right will be created after analysis. r


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Create a new folder in order to save those files in the same folder.

Icon File type

 
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Data file
 $MJDL Open the [Create New Project (Folder)] sub-window.

 *OQVU Enter the folder name.
Method file
 $MJDL Select the files to copy to the new folder.
 $MJDL The new folder is created.*1

Report format file

*1 A new folder is created on the same level of the currently opened folder in Data Explorer.
For detailed procedure to open the target folder, please refer to the Operation Guide "Appendix C Using Data Explorer".
Batch file

4 Performing Data Acquisition GCMS Data Acquisition Program

Please modify the previously created batch file for easy operation.
For details on how to create method files and batch files, refer to the Operation Guide “4.1 Creating a  
Method File”, “5.1 Creating a Method File”, “4.3.1 Creating a Batch File”and “5.2.1 Creating a Batch File”.

 
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 $IFDL Check that the window is the [Batch Table] window. 

 %PVCMFDMJDL Open the batch file to be used.
 $IFDL Check and edit the batch table.*1 
 $MJDL Overwrite and save any edits. 
 $MJDL The instrument enters the ecology mode after data acquisition ends.*2
 $IFDL Set the rinsing solvent and sample in place.
"
 $MJDL Display the confirmation message.
 $MJDL Start data acquisition.
" $MJDL Cancel data acquisition.

*1 For details on how to edit batch table, refer to the Operation Guide “4.3.2 Editing a Batch File”. 
*2 This setting can be cancelled by repeating operation 5, but always leave it set.

 
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5 Data Processing for Qualitative Analysis GCMS Postrun Analysis Program

Display the mass spectra for chosen peak and perform library search.
Register the results in the spectrum prodcess table. It allows referencing  
the results later or printing out the report.  
*1 For details on how to create reports, refer to the
Operation Guide “4.5 Printing Qualitative Analysis Reports”.
 The range dragged with
the mouse is displayed
enlarged in the MC window.

 


At peak top


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 %PVCMFDMJDL Start up the [GCMS Postrun Analysis] program.

Check that the window is the [Data Analysis] window
 $IFDL
in the qualitative processing mode.

 $MJDL Select the created folder.
At peak tailing edge
 %PVCMFDMJDL Open the data file.
 %SBH Display the peak enlarged.
 %PVCMFDMJDL Display the mass spectrum of the peak top.
 $MJDL Switch to the background processing mode.

 %PVCMFDMJDL Perform background subtraction. 
 $MJDL Open the [Similarity Search Results] sub-window. 
 $MJDL The searched compounds are switched.

 $IFDL Check while comparing the compounds.  
 $MJDL Select the compound considered to be correct. 
 $MJDL Register the selected compound to the spectrum process table.
Displayed spectrum
 $MJDL Close the [Similarity Search Results] sub-window.

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UP
 Repeat these operations to register all target components.
 $MJDL Open the qualitative table.
 $MJDL Maximize the table.
Searched compounds
 $MJDL Select the row to edit.
 $MJDL Edit the row (e.g. correct compound name).
 $MJDL Close the qualitative table.  Searched compounds

 $MJDL Overwrite and save the data file.

 $MJDL Create a report.*1

 
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6 Data Processing for Quantitative Analysis GCMS Postrun Analysis Program

Create a calibration curve and calculate the concentration of target compounds.


*1 For details on how to create reports, refer to the Operation Guide “5.4 Printing Quantitative Analysis Reports”.  

 
Identification mark


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 %PVCMFDMJDL Start up the [GCMS Postrun Analysis] program.

 $IFDL Check that the window is the [Calibration Curve] window.
Peak detection marks
 $MJDL Select a created folder.
 %PVCMFDMJDL Open the method file used for data acquisition.
 $MJDL Check the calibration curve of the target component. *3 The peak integration and peak detection are automatically performed on the chromatogram
( peak detection marks).
 $MJDL Check the chromatogram of the target component.*3
Detected peaks are identified based on retention times and ion ratios ( identification mark).
 .PEJGZ Perform manual peak integration and manual identification as required.*2
 $MJDL Overwrite and save any corrections. 
*2 For details on manual peak integration and manual identification, see page 9.


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 $IFDL Check that the window is the [Data Analysis] window in the quantitative processing mode.  
 %PVCMFDMJDL Open the data file.
 $MJDL Load the method file used for creating the calibration curve.   
 $MJDL Perform quantitative peak integration.
 $MJDL Check the quantitative results.
 $MJDL Select the target row. 
 $IFDL Check the chromatogram of the target component.*3
 .PEJGZ Perform manual peak integration and manual identification as required.
 $MJDL Overwrite and save the data file.

 $MJDL Create a report.*1

 
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How to Perform Manual Peak Integration Appendix. Performing Maintenance GCMS Data Acquisition Program

"Easy sTop" is a convenient function for

exchange of septum and insert. It shortens the
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period for stabilizing the GC/MS system after
 3JHIUDMJDL Open the popup menu.  $MJDL Open the [Vacuum Control] sub-window.
the exchange.
 $MJDL Switch to the manual peak integration mode. Perform maintenance referring to the Operation  $MJDL Start automatic stop.
 %SBH Specify the start and end of the baseline. Guide "Appendix D Maintenance" or MS
 $IFDL [Completed.] is displayed.
Navigator.
 $MJDL Select the baseline.  $MJDL Close the [Vacuum Control] sub-window.
 $MJDL The peak is integrated and identified.


  
 

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 $MJDL Open the MS Navigator.

 4FMFDU Display the maintenance procedure for the selected part.
 $IFDL Perform maintenance as described in the procedure.


 

How to Perform Manual Identification

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 3JHIUDMJDL Open the popup menu.

 $MJDL Switch to the manual identification mode.
 $MJDL The peak is identified. r
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 4ZSJOHF Thoroughly rinse with solvent before data acquisition. During operation
   4FQUVN Replace every 100 data acquisitions. Easy sTop
·Replace according to the injection mode (split, splitless).
 *OTFSU Easy sTop
·Replace when the peak shape has deteriorated or peak intensity has decreased.
·Check separation between the target component and other peaks, and replace the column
 $PMVNO with the ideal column. Vacuum system stopped
 ·Perform maintenance when the peak shape has deteriorated or peak intensity has decreased.
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·Replace when the trap current insufficient message is displayed. Vacuum system stopped
·Replace burned out filaments.
 Replace every 3000 hours of operation. Vacuum system stopped
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