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Reverse Blood Grouping Procedure: A B Serum or Plasma A RBC Reagent A B RBC Reagent B

The document provides instructions for performing a reverse blood grouping procedure to determine a patient's blood type. Two test tubes are labeled and each has drops of the patient's serum or plasma added. One drop of A RBC reagent is added to one tube and one drop of B RBC reagent is added to the other. The contents of the tubes are mixed and centrifuged before examining for hemolysis and agglutination to determine the blood type based on the results.

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michael mann
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924 views1 page

Reverse Blood Grouping Procedure: A B Serum or Plasma A RBC Reagent A B RBC Reagent B

The document provides instructions for performing a reverse blood grouping procedure to determine a patient's blood type. Two test tubes are labeled and each has drops of the patient's serum or plasma added. One drop of A RBC reagent is added to one tube and one drop of B RBC reagent is added to the other. The contents of the tubes are mixed and centrifuged before examining for hemolysis and agglutination to determine the blood type based on the results.

Uploaded by

michael mann
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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REVERSE BLOOD GROUPING PROCEDURE

1. Label 2 test tubes with specimen identifying information (patient’s name or intials

or blood donor’s number). Label one tube “a” and one tube “b”.

2. Place 2 drops of the specimen’s serum or plasma into each tube.

3. Add one drop of A RBC reagent to the “a” tube and one drop of B RBC reagent to

the “b” tube.

4. Gently shake each tube to mix the contents, and then centrifuge tubes for 15-20

seconds on high speed (3500rpm)

5. Examine for hemolysis, then gently resuspend the RBC button, and read for

agglutination.

6. Record the results on the appropriate worksheet for this test.

REVERSE BLOOD GROUPING UNKNOWN ABO GROUPING


SERUM/PLASMA TESTED AGAINTS
A CELLS B CELLS
+ + O
O + A
+ O B
O O AB

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