Ventromedial and dorsolateral prefrontal interactions underlie

Will to fight and die for a cause

An fMRI functional connectivity study

Clara Pretus1,2, Nafees Hamid1,3, Hammad Sheikh1,4, Ángel Gómez1,5, Jeremy Ginges1,4, Adolf

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Tobeña1,2, Richard Davis1,6,7, Oscar Vilarroya1,2, and Scott Atran1,7,8,9

1
Artis International, Scottsdale, AZ, USA
2
Departament de Psiquiatria i Medicina Legal, Universitat Autònoma de Barcelona, Cerdanyola
del Vallès, Spain
3
Department of Security and Crime Science, University College London, London, England
4
Department of Psychology, New School for Social Research, New York, NY, USA
5
Departamento de Psicología Social y de las Organizaciones, Universidad Nacional de
Educación a Distancia, Madrid, Spain
6
School of Politics and Global Studies, Arizona State University, Tempe, AZ, USA
7
The Changing Character of War Centre, Pembroke College, University of Oxford, Oxford,
England
8
Centre National de la Recherche Scientifique, Institut Jean Nicod – Ecole Normale Supérieure,
Paris, France
9
Gerald Ford School of Public Policy and Institute for Social Research, University of Michigan,
Ann Arbor, MI USA

Corresponding authors:

Clara Pretus Óscar Vilarroya

Dept. de Psiquiatria i Medicina legal Dept. de Psiquiatria i Medicina legal
Facultat de Medicina, Campus de Bellaterra Facultat de Medicina, Campus de Bellaterra
08193 Cerdanyola del Vallès 08193 Cerdanyola del Vallès
Barcelona, Spain Barcelona, Spain
Tlf. +34 933160449 Tlf. +34 933160485
An fMRI functional connectivity study

Abstract

Willingness to fight and die (WFD) has been developed as a measure to capture willingness to

incur costly sacrifices for the sake of a greater cause in the context of entrenched conflict. WFD

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measures have been repeatedly used in field studies, including studies on the battlefield,

although their neurofunctional correlates remain unexplored.

Our aim was to identify the neural underpinnings of WFD, focusing on neural activity and

interconnectivity of brain areas previously associated with value-based decision-making, such

as the ventromedial and the dorsolateral prefrontal cortex.

A sample of Pakistani participants supporting the Kashmiri cause was selected and invited to

participate in an fMRI paradigm where they were asked to convey their willingness to fight and

die for a series of values related to Islam and current politics.

As predicted, higher compared to lower WFD was associated with increased ventromedial

prefrontal activity and decreased dorsolateral activity, as well as lower connectivity between the

ventromedial and the dorsolateral prefrontal cortex.

Our findings suggest that WFD more prominently relies on brain areas typically associated with

subjective value (vmPFC) rather than integration of material costs (dlPFC) during decision-

making, supporting the notion that decisions on costly sacrifices may not be mediated by cost-

benefit computation.

Keywords

Costly sacrifices; Will to fight and die; sacred values; fMRI; functional connectivity

Word count: 5212

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An fMRI functional connectivity study

Introduction

In previous studies, our research group has studied beliefs and values embedded within

protracted political conflict across a number of contexts, such as the Israeli-Palestinian conflict

(Sheikh et al., 2013), the US-Iranian nuclear conflict (Dehghani et al., 2009), the Indian-

Pakistani conflict over Kashmir (Sachdeva & Medin, 2009) and the frontline battle against ISIS

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in Iraq (Gómez et al., 2017). Across these samples, respondents report high levels of

commitment to their in-group and the values, often showing willingness to personally fight and

die to defend them. Thus, the need emerged to develop a suitable measure to assess motivation

for incurring extreme personal costly sacrifices including risking one’s life and the lives of

others to defend highly cherished beliefs. To this end, willingness to fight and die (WFD) was

developed as a measure of extreme commitment in field studies of value-based conflicts,

whether involving active supporters (Swann et al., 2009; Gómez et al., 2011; Atran et al.,

2014a; Atran et al., 2014b) or frontline combatants (Gómez et al., 2017).

Behavioral field studies show that decisions over willingness to engage in costly

sacrifices for the sake of a greater cause escape instrumental reasoning and cost-benefit

computation (Ginges et al., 2011). For example, willingness to partake in political violence

compared to non-violence was mainly predicted by perceived righteousness, with little

contribution of perceived effectiveness, whereas support for military compared to diplomatic

solutions to a conflict was insensitive to quantity in terms of number of saved lives and number

of deterred enemy attacks in the future (Ginges & Atran, 2011).

Value-based choices are generally thought to be modulated by interactions between the

dorsolateral (dlPFC) and the ventromedial (vmPFC) prefrontal cortex usually favoring adaptive

behavior (Basten, Biele, Heekeren, & Fiebach, 2010; Hare, Camerer, & Rangel, 2009; Rudorf &

Hare, 2014). Whereas the vmPFC has been proposed as a global value comparator that

computes subjective value (Basten et al., 2010; Camille et al., 2011), the dlPFC is thought to

modulate value encoding during decisions that integrate costs (Hare et al., 2009) or reweight

stimulus attributes (Rudorf & Hare, 2014). However, the interplay between the dlPFC and the

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An fMRI functional connectivity study

vmPFC during value-based decisions has not yet been investigated in the context of willingness

to fight and die. Although WFD should broadly recruit brain networks associated with value-

based choice, such as the ventromedial (vmPFC) and dorsolateral prefrontal cortex (dlPFC)

(Plassmann et al., 2007; Hutcherson et al., 2012), decisions involving high compared to low

WFD might be expected to show substantial differences in the extent to which brain regions

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associated with cost-benefit calculation are recruited.

Accordingly, the goal of the present study was twofold. First, we sought to identify the

neural correlates of willingness to fight and die by comparing values receiving high versus low

willingness to fight and die ratings, especially focusing on activity in usual decision-making

neural hubs such as the dlPFC and vmPFC. Second, we endeavored to establish a functional

connectivity profile between these brain regions, respectively associated with integration of

costs during decision-making (dlPFC) and subjective value computation (vmPFC), during

proceeding of willingness to fight and die. For this purpose, we recruited a sample of male

participants of Pakistani origin living in Barcelona (Spain) who expressly supported the cause

of Kashmiri resistance to Indian rule, and then scanned them while they expressed willingness

to fight and die for a series of values related to Islam and current politics. We then analyzed

neural activity associated with high and low willingness to fight and die, and compared

functional connectivity between the dlPFC and the vmPFC in these two conditions using the

CONN functional connectivity toolbox (The Gabrieli Lab, McGovern Institute for Brain

Research). This toolbox allows quantifying the co-variance among previously selected neural

regions, or between previously selected neural regions and the rest of the brain, in a given

experimental condition.

Given that willingness to fight and die (WFD) for a value is a measure that reflects

disposition to incur extreme costly sacrifices, we predicted high compared to low WFD to be

positively correlated with brain activity that is typically associated with subjective value during

decision-making (vmPFC). Because the personal drive to fight and die for a cause often runs

independently of risks and outcomes for the self (Sheikh et al., 2016), we also anticipated lower

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An fMRI functional connectivity study

integration of costs, hence, reduced activity in the dlPFC, during high versus low WFD

decisions. For the same reason, we expected functional connectivity between the dlPFC and the

vmPFC to be weak or even negative in the high versus low WFD condition.

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Methods

Participants

The study was approved by the Institutional Review Board (IRB) Ethics Committee

according to the Declaration of Helsinki guidelines and informed consent was obtained from all

participants before taking part in the study. Data protection was a priority in this study and

complete anonymity was guaranteed.

Participants were selected by means of field interviews and surveys with a sample of

146 male respondents of Pakistani origin living in migrant marginalized neighborhoods of

Barcelona (Spain), the average age was 30.82 years old (range 18 to 62). All respondents were

Muslim, and most self-identified as Sunni. In line with the recruitment criteria, all participants

in the selected sample expressed: (1) support for a jihadist group which fights for the Pakistani

reclamation of Kashmir (2) agreement with armed jihad (3) agreement that Muslims must unite

against the West by all means necessary and (4) willingness to personally engage or facilitate

violence in the name of armed jihad (to engage in violent protest, to financial support a non-

state militant group, to join a non-state militant group or to fight and die on their own in support

of armed jihad). From those n = 45 respondents who met all three criteria in the field survey, n

= 30 agreed to participate in the fMRI study.

The final fMRI study sample consisted on male Muslim participants from Pakistani

origin living in and around Barcelona. The average age was 30.10 years old (sd = 5.37, range

from 18 to 39 years old), 51.7% of them were married, 82.7% finished secondary school and

10.3% held a Bachelor’s Degree, 72.4% reported a well below average income, and 72.4%

stated that religion was the most important thing in their lives. All but one agreed that “India

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An fMRI functional connectivity study

should have no right to ownership of Kashmir” and all of them reported a favorable or

somewhat favorable opinion of Lashkar-e-Taiba (LeT or “army of the pure”), one of the largest

militant Islamist groups in Pakistan functioning since 1993 and declared a terrorist group by the

US (2002) followed by the UN (2005). The organization fights for the liberation of Kashmir

from Indian control and the unification of all Muslim majority regions beyond Pakistan,

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pointing at the US, Israel and India as existential enemies of Islam, as reported by the SATP

(South Asia Terrorism Portal, www.satp.org). LeT has been attributed several violent attacks

including the 2008 Mumbai bombings, which caused 200 deaths and 300 injured civilians, and

the Indian parliament attacks in 2001 (see Box 1 for more details on the Kashmir conflict).

Box 1. The Kashmir conflict started in 1947 with India’s independence and its partition

from Pakistan. Although it began as a territorial dispute it has evolved into a clash between

Hindu and Muslim religious identities especially salient for the Muslim community (Sachdeva

& Medin, 2009). The first war between India and Pakistan over Kashmir in 1947 caused mass

killings of all ethnic groups and hundreds of thousands of refugees. In 1948 the UN Security

Council imposed a ceasefire, forcing Pakistan to withdraw its troops, and promised a plebiscite

for Kashmiris to democratically vote for their independence. Neither Pakistan obeyed nor did

the plebiscite take place, creating a climate of escalating violence leading to the Sino-Indian war

in 1962 and the Indo-Pakistani war in 1971. The Indian military occupation lead to the

formation of opposing Muslim militant groups, culminating in the Mujahedeen insurgency in

1989, which has caused 70000 deaths and 8000 missings to date. The most recent ceasefire

resolution between Pakistan and India was signed in 2003, although there have been several

violent attacks from both sides ever since. Currently, 43% of the territory is controlled by India,

37% by Pakistan and 20% by China. The general Pakistani view defends that Kashmir should

be independent or annexed to Pakistan, according to its Muslim majority and responding to the

Kashmiri uprising.

Procedure

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An fMRI functional connectivity study

Participants were invited to the fMRI facilities where they completed a pre-scan survey

and an fMRI task described below. The pre-scan survey was used to determine which values

were perceived as sacred (non-negotiable) and non-sacred (mundane values) by each participant

in order to ensure sufficient variability in the willingness to fight and die measure. A list of 25

candidate values was presented to participants, which was designed based on previous

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ethnographic fieldwork and included topics relevant to the sample population: “Western military

forces should be expelled from Muslim lands”, “Strict sharia should be applied in all Muslim

countries”, ”Armed jihad should be waged against the enemies of Muslims”, “India should have

no right to ownership of Kashmir”, “Current Muslim countries should be unified in a single

Caliphate”, among others (see full list in Table S1). In line with previous work on sacred values

(Baron & Spranca, 1997; Tetlock, 2003; Berns et al., 2012), a value was considered sacred if: a)

participants refused any material incentive to give it up, such as better economic conditions for

their own community, that is, they responded “No” instead of “Yes” or “Maybe” to the question

“Would you be willing to give up this value if adopting the opposite position entailed better

economic conditions for your community?”, and b) their stance was immune to social influence,

that is, they responded “No” instead of “Yes” or “Maybe” to the question “If there was a

democratic consensus over the opposite position, would you find it acceptable?” (Fiske and

Tetlock, 1997; Baron and Leshner, 2000; Ginges et al., 2007; Berns et al., 2012; Sheikh et al.,

2013). Participants had to respond “No” to both questions in order for the value to be

considered sacred. Next, participants ranked their sacred and non-sacred values by importance

and the top 6 sacred values, as well as the bottom 6 non-sacred values were selected for the

fMRI paradigm. There was a considerable amount of overlapping between each participants’

sacred value and non-sacred value sets, with the most frequently employed sacred values being

those including religious content (see Table S1). Measures of identity fusion with seven

different groups (Swann et al., 2009), including “Islam”, “Muslim Ummah”, “Pakistan”,

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An fMRI functional connectivity study

“Spain”, “Borderless Muslim Caliphate”, “Federated Muslim Caliphate” and “Close Friends”,

were also administered in the pre-scan survey. The identity fusion measure includes 5 pairs of

circles, one representing the self (a small circle) and another representing the group (a big

circle), with varying degrees of overlap between the circles. From the 5 options, only the option

representing the small circle fully inside the big circle denotes fusion. We added an additional

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response option to the original scale showing no contact at all between the two circles owing to

the reluctance of some respondents to accept any contact between the circles in previous field

studies (Gómez et al., 2017). These were intended to measure the extent to which respondents

felt “viscerally connected” to a reference group such as nation or religious community (Gómez

et al., 2017).

fMRI paradigm

The rapid event-related fMRI paradigm was designed using MATLAB 2012a (The

MathWorks, Inc., Natick, Massachusetts, United States) with Psychtoolbox extensions (Kleiner

et al., 2007). During brain scanning, participants rated their willingness to fight and die (WFD)

for each value (one value per trial) by means of a 7-point Likert scale ranging from 1 (not

willing) to 7 (extremely willing). Values were presented in Urdu (see Figure S1). Linguistic

variations of each value produced 80 different trials (40 sacred value trials and 40 non-sacred

value trials) with controlled length and sentence structure in order to increase the number of

trials within the fMRI paradigm without wording repetitions. A post-hoc analysis comparing

stimulus length in Urdu showed no differences in stimulus length neither between sacred and

non-sacred value trials (sacred values: M = 12.05 words, SD = ±.28; non-sacred values: M =

11.97 words; SD = ±.6, t(28) = .598, p = .555) nor between the conditions of interest; that is,

high and low WFD (high WFD: M = 12.08 words, SD = ±.36; low WFD: M = 12.04 words, SD

= ±.76, t(28) = .204, p = .840). Stimuli were presented randomly with jittered inter-trial

intervals in two runs. Inter-trial interval durations varied randomly from 2.5 seconds up to 12.5

seconds in 2.5-second steps. Participants were instructed to read the value and, once they knew

their response, convey their WFD making use of the 7-point Likert scale located below the

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value using left/right buttons. The participant’s response was recorded based on their button

presses by means of Psychtoolbox functions on Matlab. Pilot testing with non-selected

candidates from the same population allowed establishing a response time of 10 seconds

(reading + responding), which allowed all participants to respond to most trials. The values and

the 7-point Likert scale were always presented in the same location of the screen and the

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cursor’s starting point was always at 4 points, the center of the scale (if their response was

actually a 4, they were instructed to go either left or right and then back to 4, otherwise the trial

counted as a null). The value remained on screen until the 10 seconds were over. Keeping

stimuli in place facilitated reading and reduced confusion. Before starting the fMRI paradigms,

participants practiced the task in a computer until fully comprehending the exercise and

mastering control of the response buttons.

MRI acquisition

Images were acquired in a Siemens 3T scanner. T1-weighted images were obtained

using a FSPGR sequence (TR: 11.6 ms, TE: 4.8ms, FA: 12, matrix size: 280 x 280, 150 slices,

slice thickness: 1.00mm). An EPI-T2* sequence allowed obtaining the functional volumes, each

comprising forty 3.4 mm thick slices (TR 2500 ms, TE: 27 ms, FA: 90, matrix size: 64 x 64, 40

slices, slice thickness: 3.4 mm). Head motion was tracked by means of center of mass

measurements during image acquisition. None of the participants showed head movement above

voxel size acquisition after a post-hoc evaluation of the six head movement parameters.

SPM12 (Welcome Department of Imaging Neuroscience, London, United Kingdom)

and the Analysis of Functional Neuroimaging software were employed for the functional MRI

data analysis. Functional images were despiked (AFNI), corrected for motion-related artifacts

(SPM), normalized to MNI standard space (SPM), smoothed with a 8mm full-width-at-half-

maximum Gaussian kernel (SPM) and detrended (AFNI).

Behavioral analysis

The behavioral analysis included a paired sample t-test comparing WFD ratings in the

high WFD condition and the low WFD condition. The effect of value sacredness on willingness

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An fMRI functional connectivity study

to fight and die ratings was computed by means of a repeated measures ANOVA assessing

value sacredness as a within-subjects factor. Percentages of fused participants (6 out of 6 points)

were calculated for each identity fusion scale and correlations between identity fusion and

willingness to fight and die ratings were conducted.

Neuroimaging analysis

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Image processing was conducted using the SPM12 function package (Wellcome Trust,

University College London, UK) on MATLAB. Trials were split in high versus low WFD as a

function of each participant’s average. The first-level GLM included 2 regressors for trials

where participants conveyed either high (above each participant’s mean) or low (below each

participant’s mean) willingness to fight and die ratings. The average number of trials was 47.2

(sd = ±8.82) in the high WFD condition, and 32.8 (SD = ±8.82) in the low WFD condition. The

duration of the events spanned from stimulus onset up to the participant’s first button press, and

thus, it varied from trial to trial. Each condition was parametrically modulated by a binary

regressor with ones and zeros including whether the value at stake was sacred (1) or not (0). Of

note, the high and low WFD conditions did not always correspond to responses to sacred and

non-sacred values, respectively. That is, the high WFD condition included, at average, 77.11%

trials with sacred values (sd = 13.96%) and the low WFD condition included, at average,

12.81% trials with sacred values (sd = 13.94%). Because sacred values entail higher levels of

attitude strength, familiarity, salience and emotional intensity than non-sacred values (Pretus et

al., 2018; Hamid et al., in press), the inclusion of a value sacredness parametric regressor was

crucial to account for these confounding effects on the measures of interest (WFD). Missing

trials (no button response throughout the trial), which were, at average, 3.62 trials per

participant (SD = ±5.44) were not modelled in the GLM, and thus, they were processed as part

of the implicit baseline. In turn, in order to account for the confounding effects of motor

preparation and response, we introduced an hrf-convolved button press regressor. Six non-

convolved head movement regressors were also included.

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An fMRI functional connectivity study

Group-level effects of the high versus low WFD contrast were evaluated by means of a

t-test on individual level contrasts. In addition, we ran a second-level regression with identity

fusion with Islam and the Muslim Ummah as a predictor of neural activity in the high versus

low WFD contrast as well as in high and low versus baseline contrast, respectively. In order to

be able to capture more subtle differences in neural activity associated with identity fusion,

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identity fusion was used as a continuous variable in this analysis. Post-hoc analyses included

parameter estimate extraction of vmPFC and left and right dlPFC activity in the high WFD

versus baseline and the low WFD versus baseline contrast using Marsbar in order to illustrate

the amount of ROI activity in each condition . Reported results were corrected for multiple

comparisons using FWE correction at cluster-level, single voxel p < 0.001.

Effective connectivity analysis

The functional connectivity analysis was conducted by means of the connectivity

toolbox CONN on SPM12 (Whitfield-Gabrieli and Nieto-Castanon, 2012). To test the interplay

between brain regions associated with executive control (dlPFC) and subjective value

computation (vmPFC) during high and low willingness to fight and die, a series of anatomical

ROIs were defined using aal labels (Neurofunctional Imaging group, Université de Bordeaux).

These ROIs included the left dlPFC (left middle frontal gyrus), the right dlPFC (right middle

frontal gyrus) and the vmPFC (bilateral medial orbitofrontal gyrus). For each participant, a

generalized Psycho-Physiological Interaction (gPPI) analysis was conducted. The gPPI analysis

tests the task-modulated effective connectivity between pairs of a previously defined set of

ROIs (here: right dlPFC, left dlPFC and vmPFC) by means of a multiple regression model

incorporating task effects, the ROIs timeseries and an interaction term (PPI term) between both

parts. The effects of the high and low willingness to fight and die conditions on effective

connectivity between the bilateral dlPFC and the vmPFC were then computed and compared.

Group-level gPPI results were corrected for multiple comparisons at seed level (p < 0.05 FWE).

Results

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An fMRI functional connectivity study

The average WFD rating in the high WFD condition was 6.67 (SD ±0.48), whereas the

average WFD rating in the low WFD condition was 3.94 (SD ± 1.02). The average response

time was 5.93 seconds (SD = ±1.03), and no differences in response time were detected between

conditions (High WFD condition: M = 5.02, SD = ±1.22; Low WFD condition: M = 5.04, SD

= ±1.38; t(28) = -.089, p = .930). In addition, response time did not correlate with WFD ratings,

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neither when analyzing all trials together (r = -.032, p = .868) nor when evaluating each

condition separately (high WFD condition: r = .070, p = .718; low WFD condition: r = .042, p =

.828). Indeed, value sacredness as a within-subjects factor had a significant effect on

willingness to fight and die (F(1) = 168.612, p <.001).

Seventy-five percent of the sample was fused with Islam, 72.4% with the Muslim

Ummah, 93.1% with Pakistan, 31% with Spain, 21% with a Borderless Muslim Caliphate, 24%

with a Federated Muslim Caliphate and 79.3% with Close Friends. Identity fusion with Pakistan

was the only identity fusion measure which correlated with willingness to fight and die (r =

.388, p <.038), although this result did not survive Bonferroni correction.

fMRI signal contrasts between high versus low willingness to fight and die appear in

Table 1. The high compared to the low willingness to fight and die condition was associated

with greater activity in a cluster encompassing the anterior cingulate cortex and the left middle

orbital gyrus (T = 4.49, k = 446, p = 0.013 FWEc) and lower activity in the right inferior

parietal lobule (T = 5.49, k = 1690, p < 0.001 FWEc), right middle frontal gyrus (T = 5.47, k =

424, p = 0.016 FWEc), left superior medial frontal gyrus (T = 5.37, k = 735, p = 0.001 FWEc),

left inferior parietal lobule (T = 5.33, k = 1800, p < 0.001 FWEc) and a cluster including the left

inferior frontal gyrus (pars triangularis) and left middle frontal gyrus (T = 5.11, k = 1230, p <

0.001 FWEc) (see Figure 1A and B). Post-hoc parameter estimates analyses corroborated the

increased vmPFC activity (t(28) = 3.25, p = .003) and decreased left PFC (t(28) = -2.19, p =

.037) and right dlPFC (t(28) = -3.00, p = .006) activity in the high WFD versus baseline contrast

compared to the low WFD versus baseline contrast, although the left dPFC comparison did not

survive Bonferroni correction (see Figure 1B). Differences in willingness to fight and die scores

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in the high versus low WFD conditions correlated with neural activity differences in the left

dlPFC ( r = -.578, p = .001) and right dlPFC (r = -.456, p = 0.013), but not the vmPFC, between

conditions after Bonferroni correction (see Figure 1C).

Study participants were predominantly fused with Islam and the Muslim Ummah;

however, the available variability was negatively associated with left middle frontal gryus

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activity in the high WFD versus baseline contrast (T = 5.01, k = 489, p = .024 FWEc) and in the

high versus low WFD contrast (T = 4.38, k = 71, p = .045 FWEc after small volume correction

with the left dlPFC ROI, see Figure 2). However, identity fusion with Islam and the Muslim

Ummah did not significantly correlate with average willingness to fight and die scores (r = .141,

p = .467) nor with WFD scores in the high (r = .058, p = .766) or low (r = .066, p = .735)

willingness to fight and die condition, probably due to a limited variability of identity fusion in

this sample.

The gPPI analysis revealed a negative effective connectivity between the vmPFC as a

seed region and the bilateral dlPFC (F(2)(27) = 2.98, Intensity = 4.25, p = 0.01 FWE seed-level

corrected) when comparing the high versus low WFD conditions (see Figure 3). Results of the

reverse contrast (low > high WFD) did not survive multiple comparisons correction.

Discussion

The present study aimed to identify the neural underpinnings of willingness to fight and

die (WFD) for the sake of jihadi-related values in a sample of Pakistani participants supporting

the cause of Kashmiri resistance to Indian rule. As anticipated, decisions involving high

compared to low WFD (with respect to each participants’ average) correlated with higher

ventromedial prefrontal activity (vmPFC), a brain region typically associated with subjective

value computation during decision-making (Plassmann et al., 2007; Bartra et al., 2013).

Moreover, consistent with behavioral field studies indicating that decisions over costly

sacrifices run somewhat independently from cost-benefit computation, high compared to low

WFD negatively correlated with brain activity associated with integration of costs and stimulus

reweighting, that is, the dlPFC (Todd, Hare, Camerer, & Rangel, 2009; Rudorf & Hare, 2014).

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In addition, the effective connectivity analysis revealed a stronger negative correlation between

the vmPFC as a seed region and the bilateral dlPFC in the high versus low WFD condition.

Other neuroimaging studies on decision-making report interactions between the vmPFC

and the dlPFC during value-based choices. For example, Baumgartner et al. (2011) observed

increased dlPFC activity and higher functional connectivity between the dlPFC and the vmPFC

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during decisions where fairness and self-interest were in conflict (i.e. in participants who more

frequently made fair decisions versus participants who made more selfish decisions, but no

between-group differences in non-conflicting decisions). Similarly, Hare et al (2009) found

higher dlPFC activity and increased dlPFC-vmPFC connectivity during decisions about food

consumption in dieting “self-controllers” (participants evaluating both taste and health for each

food item) versus dieting “non-self-controllers” (evaluating taste only); and there was a

correlation between vmPFC activity and food value regardless of self-control.

Nevertheless, none of these prior studies investigated the relationship between these

brain areas in regard to decisions involving costs in human lives. Here, we found that decisions

over willingness to fight and die for a cause were modulated by interactions between the

vmPFC and the dlPFC. Particularly, the vmPFC was more active and the dlPFC, together with

other fronto-parietal areas such as the left inferior frontal gyrus and the bilateral parietal lobe,

was less active during decisions involving high willingness to fight and die for a cause. Several

studies point at the vmPFC as an integration hub that reflects the subjective value of a given

choice “all things considered” (Chib et al., 2009; Peters & Büchel, 2010; Bartra et al., 2013;

Shenhav & Greene, 2014). Moreover, the dlPFC has been associated with a myriad of functions

related to executive control, such as stimulus reweighting (Rudorf and Hare, 2014) and

integration of costs (Hare et al., 2009). More generally, the fronto-parietal network, including

areas found to be less active in the high versus low WFD conditions (inferior frontal gyrus and

the parietal lobules), has been associated with cognitive control enabling rapid and flexible

goal-oriented behavior (Marek and Dosenbach, 2018). From this perspective, our results suggest

that high (vs. low) WFD choices could involve higher subjective value, with little contribution

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An fMRI functional connectivity study

of brain regions associated with integration of costs and cognitive control (including the dlPFC).

In addition, high compared to low WFD was associated with negative effective connectivity

between the vmPFC as a seed region and the bilateral dlPFC. This finding suggests that a

greater vmPFC response contributes to bilateral dlPFC downregulation during decisions

involving high willingness to fight and die for a cause. In this vein, future research should

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clarify whether stimulating dlPFC activity effectively decreases willingness to fight and die

ratings for causes that would otherwise be associated with high willingness to fight and die

responses.

Finally, most participants were fused with Pakistan, Islam and the Muslim Ummah, in

line with studies showing identity fusion in frontline fighters, who establish family-like bonds

with fellow combatants (Whitehouse et al., 2014; Gómez et al., 2017). Available variability

revealed a positive correlation between identity fusion with Islam and the Mulsim Ummah and

decreased dlPFC recruitment during the high WFD condition (both compared to baseline and

compared to the low WFD condition) , although this did not entail higher WFD ratings. Identity

fusion is associated with commitment and costly sacrifices in defense of in-group values

(Swann et al., 2014). Identity fusion is one of the components of the “devoted actors model”,

which characterizes individuals whose personal identity is merged or “fused” with that of their

social group and to the group’s most cherished (sacred) values (Atran et al., 2014a; Atran, 2016;

Sheikh et al., 2016; Gómez et al., 2017). For similar levels of in-group commitment (similar

WFD ratings), fused participants showed a lower reliance on brain areas typically associated

with integration of costs during decisions involving costly sacrifices (left dlPFC). This finding

suggests that the effect of identity fusion on dlPFC activity alone cannot account for the

observed high willingness to fight and die making use of the present experimental design.

Our results are consistent with behavioral studies showing that decisions over

willingness to engage in costly sacrifices for the sake of a greater cause escape instrumental

reasoning and cost-benefit computation (Ginges & Atran, 2011). In those prior studies,

decisions over willingness to resort to political violence (military action) compared to non-

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An fMRI functional connectivity study

violence (diplomacy) was mainly predicted by perceived righteousness, with little contribution

of perceived effectiveness; whereas support for military compared to diplomatic solutions to a

conflict was insensitive to quantity in terms of number of saved lives and number of deterred

future attacks by the enemy (Ginges & Atran, 2011). Participants were ready to support a

military intervention to resolve a hypothetical international conflict if it meant saving the life of

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a single hostage or deterring one future attack. By contrast, diplomatic solutions were favored

only if they involved saving more than 100 hostages or 60 future attacks (Ginges & Atran,

2011).

The present study has several limitations. On one hand, values in the high and low

willingness to fight and die conditions differed in a number of ways, including emotional

intensity, familiarity and salience. This owes to the fact that sacred values (higher emotional

intensity, familiarity and salience) were more often than not included in the high WFD

condition, whereas the opposite was true for non-sacred values. This confound was addressed

by including a binary parametric regressor in the high and low conditions indicating whether the

value at stake was sacred or not. However, we understand that if a value is worth fighting and

dying for, it will almost certainly entail higher levels of emotional intensity and salience. Hence,

we tend to think that such qualities are intrinsic (rather than a confound) to high levels of

willingness to fight and die. On the other hand, it would have been interesting to compare

willingness to fight and die (WFD) and willingness to pay for goods (WTP) in the same

individuals in order to evaluate the degree of overlap between the functional clusters underlying

value-based choice in the social cause versus material goods condition. Further research is

needed to specifically test whether the pattern of dlPFC - vmPFC interactions is consistent

across social and material values, or whether discrepancies modulated by individual traits, such

as identity fusion, exist. Finally, our study is limited by the fact it focuses on a population

involved in a particular conflict. Future research should compare behavioral and neuronal

processes associated with willingness to fight and die across communities involved in different

world conflicts.

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An fMRI functional connectivity study

In sum, our findings support the notion that WFD relies on brain activity that is

generally associated with subjective value computation (vmPFC). Moreover, in line with

behavioral data showing that decisions on costly sacrifices run independently from cost-benefit

ponderation (Ginges et al., 2011), high compared to low WFD was characterized by decreased

recruitment of brain regions previously associated with integration of costs during decision-

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making, such as the dlPFC. Accordingly, our data also reveal a negative effective connectivity

between the vmPFC and the bilateral dlPFC associated with high compared to low WFD. By

providing a neurobiological perspective via fMRI measures and brain functional connectivity,

these findings are potentially relevant to identifying later stages of radicalization that involve

willingness to fight and die for a cause.

Funding

This work was supported by the Minerva Program and the Air Force Office of Scientific

Research of the U.S. Department of Defense (AFOSR FA9550-14-1-0030 DEF) and the BIAL

Foundation (Grant #163/14).

Acknowledgements

We thank everyone who participated in this study.

References

Atran, S. (2016). The devoted actor: Unconditional commitment and intractable conflict across
cultures. Current Anthropology, 57, S192–203
Atran, S., Sheikh, H., Gomez, A. (2014a). Devoted actors sacrifice for close comrades and
sacred cause. Proceedings of the National Academy of Sciences of the United States of
America, 111, 17702–3
Atran, S., Sheikh, H., Gomez, A. (2014b). For Cause and Comrade: Devoted Actors and
Willingness to Fight. Cliodynamics: The Journal of Quantitative History and Cultural
Evolution, 5
Baron, J., Leshner, S. (2000). How serious are expressions of protected values? Journal of
Experimental Psychology: Applied, 6, 183–94
Baron, J., Spranca, M. (1997). Protected Values. Organizational Behavior and Human Decision
Processes, 70, 1–16
Bartra, O., McGuire, J.T., Kable, J.W. (2013). The valuation system: A coordinate-based meta-
analysis of BOLD fMRI experiments examining neural correlates of subjective value.

17
An fMRI functional connectivity study

NeuroImage, 76, 412–27

Basten, U., Biele, G., Heekeren, H.R., et al. (2010). How the brain integrates costs and benefits
during decision making. Proceedings of the National Academy of Sciences of the United
States of America, 107, 21767–72
Baumgartner, T., Knoch, D., Hotz, P., et al. (2011). Dorsolateral and ventromedial prefrontal
cortex orchestrate normative choice. Nature Neuroscience, 14, 1468–74
Berns, G.S., Bell, E., Capra, C.M., et al. (2012). The price of your soul: neural evidence for the
non-utilitarian representation of sacred values. Philosophical Transactions of the Royal

Downloaded from https://academic.oup.com/scan/advance-article-abstract/doi/10.1093/scan/nsz034/5486105 by guest on 14 June 2019

Society B: Biological Sciences, 367, 754–62
Camille, N., Griffiths, C.A., Vo, K., et al. (2011). Ventromedial frontal lobe damage disrupts
value maximization in humans. The Journal of neuroscience : the official journal of the
Society for Neuroscience, 31, 7527–32
Chib, V.S., Rangel, A., Shimojo, S., et al. (2009). Evidence for a Common Representation of
Decision Values for Dissimilar Goods in Human Ventromedial Prefrontal Cortex. Journal
of Neuroscience
Dehghani, M., Iliev, R., Atran, S., et al. (2009). Emerging sacred values: The Iranian nuclear
program. Judgment and Decision Making, 4, 990–93
Fiske, A.P., Tetlock, P.E. (1997). Taboo Trade-offs : Reactions to Transactions That Transgress
the Spheres of Justice. Political Psychology, 18, 255–98
Ginges, J., Atran, S. (2011). War as a moral imperative (not just practical politics by other
means). Proceedings of the Royal Society of London B: Biological Sciences, 278
Ginges, J., Atran, S., Medin, D., et al. (2007). Sacred bounds on rational resolution of violent
political conflict. Proceedings of the National Academy of Sciences of the United States of
America, 104, 7357–60
Ginges, J., Atran, S., Sachdeva, S., et al. (2011). Psychology out of the laboratory: The
challenge of violent extremism. American Psychologist, 66, 507–19
Gómez, Á., Brooks, M.L., Buhrmester, M.D., et al. (2011). On the nature of identity fusion:
Insights into the construct and a new measure. Journal of Personality and Social
Psychology, 100, 918–33
Gómez, Á., López-Rodríguez, L., Sheikh, H., et al. (2017). The devoted actor’s will to fight and
the spiritual dimension of human conflict. Nature Human Behaviour, 1, 673–79
Hamid, N., Pretus, C., Vilarroya, O., et al. Neuroimaging “will to fight” for sacred values: an
empirical case study with supporters of an Al Qaeda affiliate (under review). submitted
Hare, T.A., Camerer, C.F., Rangel, A. (2009). Self-control in decision-making involves
modulation of the vmPFC valuation system. Science (New York, N.Y.), 324, 646–48
Hare, T.A., Camerer, C.F., Rangel, A. (2009). Self-Control in Decision-Making Involves
Modulation of the vmPFC Valuation System. Science, 324, 646–48
Hutcherson, C.A., Plassmann, H., Gross, J.J., et al. (2012). Cognitive Regulation during
Decision Making Shifts Behavioral Control between Ventromedial and Dorsolateral
Prefrontal Value Systems. 32, 13543–54
Kleiner, M., Brainard, D., Pelli, D., et al. (2007). What’s new in psychtoolbox-3. Perception,
36, 1–16
Marek, S., Dosenbach, N.U.F. (2018). The frontoparietal network: function, electrophysiology,
and importance of individual precision mapping. Dialogues in clinical neuroscience, 20,
133–40
Neurofunctional Imaging group, Université de Bordeaux AAL/AAL2 [online]. Available from:
http://www.gin.cnrs.fr/en/tools/aal-aal2/.

18
An fMRI functional connectivity study

Peters, J., Büchel, C. (2010). Neural representations of subjective reward value. Behavioural
Brain Research, 213, 135–41
Plassmann, H., O’Doherty, J., Rangel, A. (2007). Orbitofrontal cortex encodes willingness to
pay in everyday economic transactions. The Journal of neuroscience : the official journal
of the Society for Neuroscience, 27, 9984–88
Pretus, C., Hamid, N., Ginges, J., et al. (2018). Neural and Behavioral Correlates of Sacred
Values and Vulnerability to Violent Extremism. Frontiers in Psychology, 9, 2462
Rudorf, S., Hare, T.A. (2014). Interactions between Dorsolateral and Ventromedial Prefrontal

Downloaded from https://academic.oup.com/scan/advance-article-abstract/doi/10.1093/scan/nsz034/5486105 by guest on 14 June 2019

Cortex Underlie Context-Dependent Stimulus Valuation in Goal-Directed Choice. Journal
of Neuroscience, 34, 15988–96
Sachdeva, S., Medin, D. (2009). Group identity salience in sacred value based cultural conflict:
an examination of the Hindu-Muslim identities in the Kashmir and Babri mosque issues.
In Proceedings of the 31st Annual Conference of the Cognitive Science Society, 3111–14
Sheikh, H., Ginges, J., Atran, S. (2013). Sacred values in the Israeli–Palestinian conflict:
resistance to social influence, temporal discounting, and exit strategies. Annals of the New
York Academy of Sciences, 1299, 11–24
Sheikh, H., Gómez, Á., Atran, S. (2016). Empirical Evidence for the Devoted Actor Model.
Current Anthropology, 57, S204–9
Shenhav, A., Greene, J.D. (2014). Integrative moral judgment: dissociating the roles of the
amygdala and ventromedial prefrontal cortex. The Journal of neuroscience : the official
journal of the Society for Neuroscience, 34, 4741–49
Swann, W.B., Buhrmester, M.D., Gómez, A., et al. (2014). What makes a group worth dying
for? Identity fusion fosters perception of familial ties, promoting self-sacrifice. Journal of
Personality and Social Psychology, 106, 912–26
Swann, W.B., Gómez, A., Seyle, D.C., et al. (2009). Identity fusion: the interplay of personal
and social identities in extreme group behavior. Journal of personality and social
psychology, 96, 995–1011
Tetlock, P.E. (2003). Thinking the unthinkable: sacred values and taboo cognitions. Trends in
Cognitive Sciences, 7, 320–24
Whitehouse, H., McQuinn, B., Buhrmester, M., et al. (2014). Brothers in arms: Libyan
revolutionaries bond like family. Proceedings of the National Academy of Sciences of the
United States of America, 111, 17783–85
Whitfield-Gabrieli, S., Nieto-Castanon, A. (2012). Conn: A Functional Connectivity Toolbox
for Correlated and Anticorrelated Brain Networks. Brain Connectivity, 2, 125–41

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Table 1. Results of the high versus low willingness to fight and die contrast thresholded
at T = 3.4, p < 0.05 FWE cluster-level corrected, singel level p < 0.001.

MNI Coordinates
p-FWEc
Region Label k T(28) x y z

High > Low WFD

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L ACC 446 4.49 -4 38 0 0.013
Location not in atlas 446 4.07 26 38 2 0.013
L Mid Orbital Gyrus 446 3.52 -2 62 -2 0.013
Low > High WFD
R SupraMarginal Gyrus 1690 -5.49 56 -32 54 < 0.001
R Angular Gyrus 1690 -3.88 40 -72 48 < 0.001
R Middle Frontal Gyrus 424 -5.47 48 36 32 0.016
L Superior Medial Gyrus 735 -5.37 0 24 46 0.001
L Inferior Parietal Lobule 1800 -5.33 -54 -40 54 < 0.001
L Inferior Parietal Lobule 1800 -5.05 -36 -44 46 < 0.001
L Superior Parietal Lobule 1800 -4.54 -26 -68 62 < 0.001
L IFG (p. Triangularis) 1230 -5.11 -40 20 6 < 0.001
L IFG (p. Triangularis) 1230 -4.99 -52 14 32 < 0.001
L Middle Frontal Gyrus 1230 -3.50 -44 34 32 < 0.001

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An fMRI functional connectivity study

Figure 1. Results of the high versus low willingness to fight and die contrast. (A)
The high versus low willingness to fight and die conidition yielded higher activity in a
cluster including the anterior cingulate cortex (ACC) and the vmPFC (in red-yellow)
and lower activity in the superior medial frontal gyrus. the right middle frontal gyrus.
left inferior frontal gryus (pars triangularis). and bilateral inferior parietal lobule (in
blue-green). Results thresholded at T = 3.4. p < 0.05 FWEc. singel level p < 0.001. (B)
Parameter estimates of vmPFC activity were higher in the high WFD vs. baseline
condition compared to the low WFD vs. baseline condition (t(28) = 3.25. p = 0.003),
whilst those of the bilateral dlPFC were lower (left dlPFC: t(28) = 2.19. p = 0.037; right

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dlPFC: t(28) = 3.00. p = 0.006) in the same contrast. (C) Between-conditions difference
in willingness to fight and die ratings correlated with between-conditions left dlPFC ( r
= -.578, p = .001) and right dlPFC (r = -.456, p = 0.013), but not with vmPFC activity
difference, after Bonferroni correction for 3 tests (one per ROI).

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An fMRI functional connectivity study

Figure 2. Correlations between neural activity and identity fusion measures: (A)
surface projection of the left middle frontal gryus activity predicted by fusion with
Islam and the Muslim Ummah in the high WFD versus baseline contrast (T = 5.01, k =
489, p = .024 FWEc) and in the high versus low WFD contrast (T = 4.38, k = 71, p =
.045 FWEc after small volume correction with the left dlPFC ROI), (B) Scatterplot of
the correlation between the fusion score and left middle frontal gryus parameter
estimates found in the high WFD versus baseline contrast (r = -.659, p < .001) and the
high versus low WFD contrast (r = -.627, p <.001), (C) results table of second-level
GLM using identity fusion with Islam and the Muslim Ummah as predictor of brain

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activity in the high and low WFD versus baseline contrasts, respectively, and the high
WFD versus low WFD contrast.

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An fMRI functional connectivity study

Figure 3. Effective connectivity analysis results: (A) Parameter estimates of the high
WFD effects (dark red) and the low WFD effects (light red) on the effective
connectivity between each pair of ROIs including the left and right dlPFC and the
vmPFC (“[seed region] – [target region]”), (B) 3D display of the effective connectivity
differences between the high and low WFD conditions after FWE seed-level correction
(left lateral and superior view), and (C) effective connectivity results table comparing
high > low WFD including a negative correlation between the vmPFC as a seed region
and the bilateral dlPFC, no significant results were found in the reverse contrast (low >
high WFD).

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