Histology of Muscles: Learning Objectives

HISTOLOGY OF MUSCLES
LEARNING OBJECTIVES
 By the end of this lecture, the students should be able to understand:
 Be able to distinguish the three types of muscle at the light and electron microscope levels,
including distinctive features of each muscle fiber.
 Describe the structural basis of muscle striations.
 Know the structural elements that harness muscle contraction to the movement of a body part.
 Know the function and organization of the connective tissue in muscle.
Muscle Function
1. Contraction for locomotion and skeletal movement
2. Contraction for propulsion
3. Contraction for pressure regulation
Muscle classification
1. Muscle tissue may be classified according to a morphological classification or a functional
classification.
 There are two types of muscle based on the morphological classification system
1. Striated
2. Non striated or smooth.
 Functional classification
 There are two types of muscle based on a functional classification system
1. Voluntary
2. Involuntary.
Types Of Muscle
 Types of muscle: there are generally considered to be three types of muscle in the human body.
1. Skeletal muscle: which is striated and voluntary

2. Cardiac muscle: which is striated and involuntary
3. Smooth muscle: which is non striated and involuntary
Characteristics of skeletal muscle

1. Skeletal muscle cells are elongated or tubular.
2. multiple nuclei located on the periphery of the cell.
3. Skeletal muscle is striated.
Characteristics Of Cardiac Muscle

1. Cardiac muscle cells are not as long as skeletal muscles cells and often are branched.
2. Cardiac muscle cells may be mononucleated or binucleated.
3. nuclei are located centrally in the cell.
4. Cardiac muscle is also striated.
5. In addition cardiac muscle contains intercalated discs.
Characteristics Of Smooth Muscle

 Smooth muscle cell are described as spindle shaped. Wide in the middle and narrow to almost a
point at both ends.
 Single centrally located nucleus.
 Smooth muscle cells do not have visible striations although they do
 Contains the same contractile proteins as skeletal and cardiac muscle, but laid out in a different
pattern.
Muscle Terminology
Myofiber Or Myocyte: a muscle cell
1. Sarcolemma: the plasma membrane of a muscle cell
2. Sarcoplasm: the cytoplasm of the muscle cell
3. Sarcoplasmic reticulum: the endoplasmic reticulum of a muscle cell
4. Sarcosome: the mitochondria of a muscle cell
5. Sarcomere: the contractile or functional unit of muscle

There are specialized invaginations of the sarcolemma that run transversely across the cell known as T
tubules (transverse tubules).
Terminal Cisternae are sac-like regions of the sarcoplasmic reticulum.
The terminal cisternae act as calcium storage sites. The calcium ions stored in the terminal cisternae are
essential in muscle contraction.
Microscopic Structure of Skeletal Muscle

 The entire muscle is surrounded by a connective tissue called the epimysium.
 Fascicles are bundles of individual muscle cells (myofibers or myocytes).
 These bundles are surrounded by a connective tissue sheath called the perimysium.
 Each fascicle is made up of several muscle cells known as myocytes or myofibers or delicate
muscle fibers.
 Each muscle Cell is surrounded by a connective tissue sheath known as the endomysium
Organization Of Skeletal Muscle Fibers

The sarcomere is the repeating unit of a myofibril in a muscle cell, composed of an array of overlapping
thick and thin filaments between two adjacent Z discs.
The muscle fibers show the cross-section of alternating light and dark bands. The dark bands are called A
bands and light bands are called Ibands.
In electron microscope, each I band is bisected by a dark transverse Z line.
The sarcomere extends from Z line to Z line and is about 2.5μm in resting muscles.
Sarcomere
Thick and Thin Filaments

 Sarcomere pattern is due to two types of filaments, thick and thin.
 Thick filaments occupy A band.
 The I band consists of thin filaments.
 The thick A band overlap the thin I band filaments
 The A band shows the presence of light zones in center, the H band containing only Myosin
molecules.
 The H band is bisected by „M‟ line.
Contraction.
Striated muscle filaments contain 4 types of proteins; Actin, Tropo-myosin, Troponin and Myosin.
Thin filaments are composed of First three proteins and thick filament of Myosin.
Thin filaments are formed by filamentous protein called as F-actin. F-actin is composed of two strands of
G-actin.
Thin filaments also contain other proteins Tropo-myosin and Troponin.
 MYOSIN: myosin is a much larger complex, can be dissociated into two pairs of heavy and light
chains.
 Heavy chains are thin rod like molecules that form the Heads which are ATP binding sites able to
bind Actin.
 In each thick filament several hundred molecules are arranged.
 Analysis of thin section of Striated muscles show presence of cross bridges b/w thin and thick
filaments. The bridges are at head of myosin molecules involved in the conversion of chemical
energy into mechanical energy.
Blood supply of skeletal muscles

Rich capillary supply.
Nerve supply.
One motor nerve ending form a somatic nerve.
One nerve fiber innervate a single muscle fiber.
A motor nerve fiber and muscle fiber are collectively known as a motor unit.
Cardiac Muscles
1. Involuntary .
2. Striated,
3. Nucleus is oval.
4. Found in Myocardium.
5. Present in the form of chains.

6. Cells within each chain bifurcate or branch giving an appearance of syncytial network.
7. Unique character of cardiac muscle is presence of darkly stained transverse lines that cross the
chain of cardiac cells, these bands often follow an irregular step like course and are known as
Intercalated discs.
 The T-tubule are large in ventricular muscles..
 Cardiac t-tubule are found at the level of „Z‟ band, rather than A-I junction.
 The sarcoplasmic reticulum is not well developed.
 Triads are not common in cardiac muscles but T-tubule is associated with one lateral
endoplasmic reticulum cisternae are called Diads.
 In old age, lypofuscin pigment is deposited around nucleus.
Smooth Muscles
1. Smooth muscle is found in stomach, intestine, urinary and genital tract.
2. Fusiform cells.
3. Striations are longitudinal only.
4. Longitudinal striations run throughout length.
5. Single central nucleus.
6. Sarcoplasmic reticulum is rudimentary.
7. T-tubules are not present.
8. Characteristic contractile activity depends upon the Actin and Myosin filaments.
9. There is little connecting tissue b/w adjacent myofibrils.

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HISTOLOGY OF MUSCLES

 Describe the structural basis of muscle striations.

 Know the function and organization of the connective tissue in muscle.

2. Contraction for propulsion

3. Contraction for pressure regulation

2. Non striated or smooth.

 There are two types of muscle based on a functional classification system

1. Skeletal muscle: which is striated and voluntary

3. Smooth muscle: which is non striated and involuntary

Characteristics of skeletal muscle

2. multiple nuclei located on the periphery of the cell.

3. Skeletal muscle is striated.

Characteristics Of Cardiac Muscle

2. Cardiac muscle cells may be mononucleated or binucleated.

3. nuclei are located centrally in the cell.

4. Cardiac muscle is also striated.

5. In addition cardiac muscle contains intercalated discs.

Characteristics Of Smooth Muscle

 Single centrally located nucleus.

 Smooth muscle cells do not have visible striations although they do

1. Sarcolemma: the plasma membrane of a muscle cell

2. Sarcoplasm: the cytoplasm of the muscle cell

3. Sarcoplasmic reticulum: the endoplasmic reticulum of a muscle cell

4. Sarcosome: the mitochondria of a muscle cell

5. Sarcomere: the contractile or functional unit of muscle

Terminal Cisternae are sac-like regions of the sarcoplasmic reticulum.

Microscopic Structure of Skeletal Muscle

 Fascicles are bundles of individual muscle cells (myofibers or myocytes).

Organization Of Skeletal Muscle Fibers

In electron microscope, each I band is bisected by a dark transverse Z line.

Thick and Thin Filaments

 Thick filaments occupy A band.

 The I band consists of thin filaments.

 The thick A band overlap the thin I band filaments

Thin filaments also contain other proteins Tropo-myosin and Troponin.

 In each thick filament several hundred molecules are arranged.

Blood supply of skeletal muscles

One motor nerve ending form a somatic nerve.

One nerve fiber innervate a single muscle fiber.

5. Present in the form of chains.

 The T-tubule are large in ventricular muscles..

 The sarcoplasmic reticulum is not well developed.

 In old age, lypofuscin pigment is deposited around nucleus.

3. Striations are longitudinal only.

4. Longitudinal striations run throughout length.

5. Single central nucleus.

6. Sarcoplasmic reticulum is rudimentary.

7. T-tubules are not present.

9. There is little connecting tissue b/w adjacent myofibrils.

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