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Lab Report 2 1

The document describes an experiment analyzing phosphate levels in different samples. It includes calibration of the spectrophotometer using ammonium vanadomolybdate. Measurements were taken of a fertilizer sample and a Gatorade drink. The fertilizer was found to contain 1.8% phosphate with a standard deviation of 0.0007. The Gatorade contained 260 mg of phosphate with a standard deviation of 4 mg. Calculations and absorption spectra are provided.

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Elen Mari
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179 views

Lab Report 2 1

The document describes an experiment analyzing phosphate levels in different samples. It includes calibration of the spectrophotometer using ammonium vanadomolybdate. Measurements were taken of a fertilizer sample and a Gatorade drink. The fertilizer was found to contain 1.8% phosphate with a standard deviation of 0.0007. The Gatorade contained 260 mg of phosphate with a standard deviation of 4 mg. Calculations and absorption spectra are provided.

Uploaded by

Elen Mari
Copyright
© © All Rights Reserved
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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Nana Mirotadze

Lab partner: Elene Ivaniashvili

G3

30/05/2019

Chem 201, Experiment 2

Analysis of Phosphate

Procedure: I followed procedure of Chem 201 lab manual. Prelab pages 3-4.

Experimental data:

Part 1 calibration

Table 1, Phosphate+ammonium vanadomolybdate

Wavelength (nm) absorbance


375 1.33
385 0.99
395 0.77
405 0.60
415 0.48

Absorbance vs Wavelength
1.4

1.2

1
absorbance

0.8

0.6

0.4

0.2

0
370 375 380 385 390 395 400 405 410 415 420
wavelength (nm)

The highest absorbance was at 375 nm it was 1.33 (AT)

Table 2, ammonium vanadomolybdate


Absorbance at 375 nm (AR) 0.16
Concentration of stock solution (M) 2.5x10^-4
Part 2

Fertilizer 100g/L

At first, we prepared 1:30 solution but absorbance was more than 1.7 it was 2.54, next we prepared 1:40
solution it was 2.50 so next we prepared 1:80 solution absorbance was 1.75 and finally we prepared 1:160
solution by adding 4mL water to 3rd 4mL solution and absorbance was 0.93.

Table 3

concentration Absorbance
1:30 2.54
1:40 2.50
1:80 1.75
1:160 0.93
Absorbance of 1:160 solution table 4

Trial 1 0.93
Trial 2 0.92
Trial 3 0.93

Part 3

Energy drink Gatorade Frost blue drink solution 1:30 absorbance Table 4

Trial 1 0.64
Trial 2 0.63
Trial 3 0.63

Calculated results:

Part 1 calibration

Absorbance of phosphate complex AP 1.17


𝜀𝑙 of phosphate complex M-1 4.76x10^3

Part 2 fertilizer

Trial 1 Trial 2 Trial 2


AP 0.77 0.76 0.77
Concentration of 1.6x10^-4 1.58x10^-4 1.6x10^-4
diluted solution (1:160)
(M)
Concentration of 0.026 0.0252 0.026
original solution (M)
g/L of P2O5 1.8 1.79 1.8
% P2O5 1.8 1.79 1.8
average 1.8
Standard deviation ±7x10^-3

Part 3 Gatorade frost 946mL

Trial 1 Trial 2 Trial 3


AP 0.48 0.47 0.47
Concentration of 1.0x10^-4 9.8x10^-5 9.8x10^-5
diluted solution (1:30)
(M)
Concentration of 3.0x10^-3 2.9x10^-3 2.9x10^-3
original solution (M)
Moles of PO43- in the 2.8x10^-3 2.7x10^-3 2.7x10^-3
bottle
Mass of PO43- (mg) 2.6x10^2 2.56x10^2 2.56x10^2
Average mass of PO43- 2.6x10^2
(mg)
Standard deviation ±4

Sample calculations

Part 1 calibration

𝐴𝑝 = 𝐴 𝑇 − 𝐴𝑅 = 1.33 − 0.16 = 1.17


𝐴𝑃 1.17
𝜀𝑙 = = = 4.8 × 103 𝑀−1
𝑐 2.5 × 10−4 𝑀
Part 2 fertilizer

𝐴𝑃 = 0.93 − 0.16 = 0.77


𝐴𝑃 0.77
𝑐(𝑑𝑖𝑙𝑢𝑡𝑒𝑑) = = = 1.6042 × 10−4 𝑀
𝜀𝑙 4.8 × 103 𝑀−1
𝑐(2) × 𝑉(2) 1.6 × 10−4 𝑀 × 160𝑚𝑙
𝑐1 (𝑜𝑟𝑖𝑔𝑖𝑛𝑎𝑙) = = = 0.026𝑀
𝑉(1) 1𝑚𝑙
𝑔 1𝑚𝑜𝑙 P2 O5
𝑜𝑓 P2 O5 = [PO4 3− ] × 𝑚𝑜𝑙𝑎𝑟 𝑚𝑎𝑠𝑠 𝑜𝑓 P2 O5 ×
𝐿 2 𝑚𝑜𝑙 PO4 3−
141.9𝑔 1 1.8𝑔
= 0.026𝑀 × × =
𝑚𝑜𝑙 2 𝐿
𝑔
𝑜𝑓 P2 O5 1.8𝑔/𝐿
% P2 O5 = 100 × 𝑔 𝐿 = 100 × = 1.8%
𝑜𝑓 𝑓𝑒𝑟𝑡𝑖𝑙𝑖𝑧𝑒𝑟 100𝑔/𝐿
𝐿
1.8% + 1.79% + 1.8%
𝐴𝑣𝑒𝑟𝑎𝑔𝑒(%𝑃2𝑂5) = = 1.8%
3

(1.8 − 1.8)2 + (1.8 − 1.79)2 + (1.8 − 1.8)2


𝑠𝑡𝑎𝑛𝑑𝑎𝑟𝑑 𝑑𝑒𝑣𝑖𝑎𝑡𝑖𝑜𝑛 = √ = 7 × 10−3
2

Part 3 Gatorade Frost blue drink

𝐴𝑃 = 0.64 − 0.16 = 0.48


𝐴(𝑝) 0.48
𝑐(𝑑𝑖𝑙𝑢𝑡𝑒𝑑 𝑠𝑜𝑙𝑢𝑡𝑖𝑜𝑛) = = = 1.0 × 10−4 𝑀
𝜀𝑙 4.8 × 103 𝑀−1
1.00 × 10−4 𝑀 × 30𝑚𝑙
𝑐(𝑜𝑟𝑖𝑔𝑖𝑛𝑎𝑙 𝑠𝑙𝑢𝑡𝑖𝑜𝑛) = = 3.0 × 10−3 𝑀
1𝑚𝑙
𝑛(𝑃𝑂43− ) = 0.946𝐿 × 3.0 × 10−3 𝑀 = 2.8 × 10−3 𝑚𝑜𝑙
94.97𝑔
𝑚(𝑃𝑂43− ) = 2.8 × 10−3 𝑚𝑜𝑙 × = 0.26𝑔
𝑚𝑜𝑙
1000𝑚𝑔
0.26𝑔 × = 2.6 × 102 𝑚𝑔
1𝑔
Average mass=2.6x10^2mg

(260 − 260)2 + (260 − 256)2 + (260 − 256)2


𝑠𝑡𝑎𝑛𝑑𝑎𝑟𝑑 𝑑𝑒𝑣𝑖𝑎𝑡𝑖𝑜𝑛 = √ =4
2

Discussion

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