Rev A - Text - USB
Rev A - Text - USB
Alliance 2695
Table of Contents
Section Title
1 Alliance Overview
2 Theory of Operation
3 Configuration
4 Direct Functions
5 Injector rebuild
6 Alliance Diagnostics
12 Method Development
The power supply is short circuit protected and does not have
external replacement fuses. The power supply is reset by power
cycling the instrument.
Analog I/O - Input; stop flow, hold inject and output; inject start,
chart out,4 switches, run stop.
The Analog Input / Output connections are the same on all the
Alliance Separations Modules.
Outlet to Column: 1/16 inch, OD, 0.009 inch ID stainless steel, Red
Tag
Degassing efficiency is based upon the solvent gas load and the
length of time the solvent remains in the chamber. Degassing
efficiency drops off above 4.5 ml/minute flow rate through a chamber.
The Gradient Proportioning Valve (GPV) has 4 solenoid valves, one for
each solvent reservoir (A,B,C,D). When a solvent valve is open, flow
from that solvent reservoir is allowed through the manifold to the
common outlet. From the common outlet the flow travels to the inlet
check valve of the Primary Head.
The line from the degasser to the “D” port has been removed to show
the threaded fitting.
3. Head nut
4. Locking ring
FIFO - First in, first out results in mixing in heads. Gradients run
smoothly.
The above slide shows the relative piston velocities for the primary
and accumulator pistons in the solvent management system for the
2695. The solvent management system components work together to
deliver solvent in the following sequence.
3 - Sample Carousel
4 - Carousel Carrier
5 - Position Sensors
6 – Z-axis motor
1 - Injector Motor
2 - Needle
3 - Seal Pack
4 - Position Sensors
When the High Pressure Motorized Valve (HPMV) is closed, the cross
port in the spool is outside the flow path.
When the valve opens, the cross port in the spool is pulled back
between the valve seals and flow can exit through the nose cap.
Because of the restrictor loop between Valve 1 and the injector, 95%
of the flow will go through the sample loop and 5 % will go through
the restrictor.
The Sample Loop Volumes applies only to this part of the purge cycle.
cycle
In the second part of the purge cycle, V1 opens, 95% of the flow goes
through the connection from V1 to V2.
The syringe drives to its lower limit (Full position), reverses direction
again and drives to its upper limit (Empty position), then returns to
its Home position.
100% flow
With V3 closed,
— the needle lowers into the vial.
— V4 closes. The needle wash pump stops.
— The syringe plunger lowers pulling sample into the needle (27 motor steps / µL
for the 250 µL syringe).
If the sample volume is greater than the syringe can draw (60% of
full capacity),
— V2 closes,
— V3 opens,
— The syringe expels the drawn volume,
— V3 closes,
— V2 opens and the syringe draws the remaining volume.
When the total sample volume has been drawn into the sample loop,
— the needle rises until the port is in the lower seal,
— V3 opens,
— V2 closes,
— the syringe expels the drawn volume.
— V3 closes.
— The needle rises until the port is between the upper and lower seals. The sample
loop pressurizes.
— V1 opens, 95% of the flow goes through the sample loop delivering the sample
to the column.
Syringe Size dialog box - Select the syringe size installed in the
Separations Module.
Verify the volume of the physical syringe and the volume displayed in
the Syringe Size: match.
The Loop Size: will accept any value from 0 to 999999 µL.
Printer dialog box - Selects the type of printer and the baud (data
transfer) rate.
Note: If you set the printer to “None”, you must select “Disable
Reporting” in the Report Options dialog box.
The baud rate set on your printer and the Alliance must match.
Report Options dialog box. - Select any option and press any numeric
key to enable or disable the option
Press Enter. An “X” in the box indicates the option is enabled.
Press Set
To enter a name, press the screen key for the appropriate letter
group, that group expands.
Press the appropriate letter, you will return to the text entry page.
The letter K was entered and the screen returned to the first text
entry page.
Choices on pull down menus are Ignore, High, Low (TTL Logic).
Logic choices are AND, OR
Status Page Layout dialog box - Allows you to enable (see) or disable
(hide) various status boxes on the Status Page. Boxes can be raised
or lowered as to placement on the Status Pages.
Highlighting a status box using the cursor arrows on the front panel
and press a numeric key to toggle enable/disable.
The Status page Layout menu scrolled to see the last two choices.
Note: Only the initial conditions in the method you select are used.
In this section we cover Dry Prime, system Prime, Wet Prime, and
Purge Injector in detail.
Press Continue.
The timer in the upper right of the window shows the time remaining
until the dry prime flow ends.
Press OK.
Select an appropriate
Flow rate
% composition of mobile phase(s)
Press Next
At the end of the specified time the flow stops and the unit returns to
the idle mode
mode.
Allow the degasser to run for 5 - 10 minutes with no solvent flow.
At this point you can either press the Resume Prime key to continue
the Wet Prime or press the Abort Prime key to end the Wet Prime.
Once the Fluid Handling Unit has been primed, turn the Degasser ON.
The 2690 degasser modes are Disabled (Off), Normal (timed cycle),
and Continuous. A 2690 Separations Module with a Performance+
degasser should be run only in Continuous mode.
Press OK.
Press Exit and then press any Numeric Key, an “X” will fill the
“Compression check?”? box.
Press OK.
This shows the first step of the purge, note the positions of the
valves.
This shows the empty syringe step of the purge, note the positions
of the valves.
This section covers removing the injector, rebuilding the Seal Pack,
replacing the needle, and re-installing the injector into the
Separations Module.
Before removing the injector, power down the unit and remove the
power cord from the back of the instrument. This will protect the
PCB’s.
Remove the cover by loosening the captive Phillips screw in the upper
left corner of the syringe compartment. (left photo above)
Remove the right side panel by removing the two Phillips screws in
the dimples near the front edge of the side panel.
With the right side panel removed, the injector and valves are visible.
Remove the needle wash lines. They are press fit over the stainless
steel tubes.
Note: The needle wash inlet line (green) is connected to the upper
needle wash housing.
The power and sensor cables for the injector are dressed along the
2695 chassis to prevent their interfering with moving parts. The
cables are held in place with nylon clips.
Start at the injector and follow the cables to their connectors on the
driver PCB.
Disconnect the sensor cable from the front edge of the PCB.
Both cables are keyed and have locking mechanisms holding them in
their respective connectors.
The motor and sensor cables have latches on them to prevent the
connector from coming loose due to vibration.
Lift the injector up and slightly back to remove it from the instrument.
Note: Do not lift the assembly too high or the restrictor loop will bend
out of shape. Although this will not effect the injector’s functioning,
the bent tubing may make re-installing the assembly into the
instrument difficult.
The Phase II injector restrictor loop and outlet line can be removed
without removing the injector from the chassis.
Turn the injector assembly over and place it on its motor to position
the seal pack up.
Pull the needle back by turning the lead screw until the slide is
touching the connector on the motor shaft.
You must remove the frit retainer on the 2695 (Performance Plus or
Phase II) needle wash housing before removing the mounting screws.
The Seal Pack is mounted to the Phase II Injector by four (4) M3 T10
TORX bolts.
This view shows the three assemblies which make up the seal pack.
The Guide Tube is inserted through the center hole of three (3) on the
Performance Plus seal body and the center hole of five (5) on the
Phase II seal body.
Manually turn the motor shaft to drive the needle forward until the
seal pack just lifts away from the injector base plate.
Hold onto the seal pack and turn the motor shaft to pull the needle
out of the seal pack.
The new needle wash filter installed in the new frit retainer.
Remove the frit retainer from the lower needle wash body and
remove the needle wash filter.
Place the lower seal wash body frit retainer side down and, using the
plastic side of the seal removal tool (WAT039802) remove the seal.
The tool on the left is as it comes from the molder. The tool on the
right has been “field modified” so it can be used to install the plunger
wash seals.
Wet the new seal (WAT270668) and seal wash body thoroughly with
methanol or IPA and insert seal into the lower seal wash body, spring
side facing in using a modified seal insertion tool (WAT270969).
Using the metal end of the seal removal tool (WAT039802), remove
the high pressure seals from the seal body.
It will be necessary to insert the removal tool at least two full turns
into the seal. These seals require a strong tug to remove them from
the body.
Tools for installing the seals (WAT034497) are in the 715 valve rebuild
kit (WAT045427)
— Seal Punch - WAT045458
— Seal Guide - WAT045459
Place the seal guide on the seal body and wet with methanol or IPA
Insert the seal into the seal guide and press down. It takes a
considerable amount of force to seat the seal in the seal body.
Remove the frit retainer from the upper seal wash body and remove
the needle wash filter and TFE washer from the frit retainer.
Using the metal end of the seal removal tool (WAT039802) remove
the upper spacer from the upper seal wash body.
Insert a new TFE washer (WAT077321) and needle wash filter frit
(WAT072324) into the frit retainer.
Turn the upper needle wash housing upside down and screw the
assembled parts into the upper seal wash body finger tight.
Insert the guide tube into the housing after wetting it with Mesh or
IPA.
Use a 5/16” wrench to loosen the compression screw and remove the
old needle.
Use the old needle as a guide. Place the new compression screw
(WAT025313) and ferrule (WAT022330) onto the pointed end of the
old needle.
NOTE: Do not put the compression screw or ferrule over the pointed end
of the new needle.
Insert the pointed end of the old needle into the fitting in the injector
slide and tighten finger tight. Remove the old needle.
Holding the new needle by the blunt end, insert the blunt end into the
compression screw and ferrule which are aligned. Ensure the blunt
end is bottomed out in the fitting.
Tighten the compression screw one full turn to seat the ferrule onto
the needle.
Align the needle by rotating the needle so the port in the needle is
aimed at the edge of the notch in the injector base plate for the seal
pack/injector.
This will ensure the port in the needle is aligned with the outlet port
of the seal pack to minimize band spreading.
Use two 5/16 inch wrenches to loosen or tighten the needle fitting on
the Phase II injector assembly.
Insert the new needle in the Phase II injector the same way as in the
standard injector (pages 40-43)
Align the needle by rotating the needle so the port in the needle is
aimed at the edge of the notch in the injector base plate for the
Phase II seal pack/injector.
This will ensure the port in the needle is aligned with the outlet port
of the seal pack to minimize band spreading.
Wet the seal pack with methanol or IPA and slide it over the needle.
Install the three screws holding the seal pack to the injector base
plate.
The new Phase II seal pack can be mounted to the old style injector
assembly using the three Phillips head screws that held the old seal
pack in place.
Screw the frit retainer, with the new filter frit, into the lower seal
wash housing finger tight.
Turn the lead screw to re-set the sensor flag between the upper and
lower sensors.
Reinstall the injector into the 2690/2695. Follow pages 5:16 to 5:8
(reverse of removing)
Helpful hint: Leave the cover and side panel off until all testing has
been successfully completed.
Main Screen
Press Diag, the diagnostics menu screen is displayed.
Transducers:
— System = accumulator pump
— Primary = primary pump
— Sample = sample loop of injector
Press Prime SealWsh, the Prime Seal Wash dialog box is displayed.
Press Start.
Seal wash pump cycles once per second. Each pulse is 200 ml.
Priming may require applying a vacuum to the seal wash outlet.
Press Start
Note: The values seen were generated when the Purge Injector was
done in the Status-Direct page in this course.
Press Close to accept the new values and return to the Diagnostics
screen.
Press Start, the function starts and runs for 0.5 minutes. It may be
necessary to elevate the needle wash reservoir and run the test more
than once to prime the needle wash. A spray of liquid will come out
the yellow line when the needle wash pump is primed.
When you first start the 2690 / 2695, after you change or rebuild the
seal pack, or as a check if you get a compression failed error.
Press Start.
Start
— Top 30 - 80 30 - 50 typical
— Bottom 80 - 200
— Width(Bottom - Top) 50 - 120 70 - 90 typical
The Adjust Seals dialog box (idle) screen showing the new and
previous values.
Diagnostics screen
Place “X”, by typing any number, in the Needle Wash Pump block to
turn it on.(Valve and Needle Wash Pump states changed by pressing a
number key.)
GPV menu
— GPV - Can be set to Solvent A, B, C, or D
Sensors menu
If the instrument continually gives a “Missing Vial” error, the vial
sensor may be adjusted for greater sensitivity
To make this adjustment:
— 1. Remove the top cover.
— 2. Go to the Sensor Page ( Main Page, Diag, Other Tests, Sensors)
— 3.Observe the two LED
LED’s
s on the encoder box on the Fluidics PCB.
• The Green LED should be blinking continuously.
• The Yellow LED should blink when it sees light (no vial) and OFF when the light beam
is broken.
— 4. Remove the carousel and place your hand in the sample compartment to
break the light beam.
— 5. Turn the adjustment pot slowly counter-clockwise until the Yellow LED begins
to blink.
Note: This adjustment is effective to increase the vial sensor sensitivity
to consistently sense vials, however, the opposite is not true. You
cannot decrease the sensitivity so the device will not sense a vial
when the unit always sees a vial.
Required Materials
— Pin plug (WAT025566)
— Wrench, 5/16-inch, open end
— Wrench, 5/8-inch, open end (or adjustable)
— Waste container
Maximum decay rate 300 psi/min (below rev.2.02), 600 psi/min (rev.
2 02 and higher) taken from 1 to 1.4
2.02 1 4 minutes after the pump arrives
at 5000 psi. (Cannot be under 4400 psi at the end of 1.4 min.)
While acquiring the decay rate the pressure in the primary may not
rise above 100 psi. This would indicate a leak in the accumulator
check valve.
At the end of 1.4 minutes, if the accumulator passes, the primary test
starts.
The pressure must reach 4000. Pressure must reach this level using
20% of the max stroke length of the plunger.
Maximum decay rate 300 psi/min (below rev 2 2.02),
02) 600 psi/min
taken from 2.5 to 2.9 minutes after the pump arrives at 5000 psi.
(Can not be under 3400 psi at the end of 2.9 min.)
During the primary test the pressure in the accumulator may not drop
below that in the primary.
Press Continue.
Perform the valve leak test whenever you suspect leakage in the
sample management valves or the seal pack, or you perform
maintenance on the valves.
Required Materials
— Pin plug (WAT025566)
— Union (WAT097332)
— Wrenches, 5/16-inch, open end, two (or one 5/16-inch and one adjustable)
— Waste container
For the Valve Leak tests the pressure limit can be set by the operator.
Press Continue.
This test option checks for solvent leaking by the upper portion of the
lower seal of the injector.
This test option checks for solvent leaking by the lower portion of the
lower seal of the injector.
The Inputs and Output diagnostic allows you to view the status of
input and output connections.
The Keypad diagnostic allows you to test the keys on the front panel
of the Separations Module. The corresponding key in the diagnostic
changes appearance when the front panel key is pressed.
Use this test if you experience problems with the carousel carrier
system.
If there is no vial in the position the carousel is sent to, the above
message is displayed.
Press Start.
Materials required
— Rebuild kit WAT45426 (contains seal body with seals) or
— Kit WAT45427 (1 time) and WAT45424 rebuild kit (w/o SS seal body) that
contains just the seals, spool, and O ring.
— Torx screwdriver (T-10)
( )
— Wash bottle with MeOH or IPA
— Waste container
Use the 5/16 inch open end wrench to remove the three fluid fittings
on the valve to be rebuilt. This picture shows Valve 2.
There are 4 Torx screws (T-10) holding the valve assembly together.
This photo shows the alignment pin in the valve housing and the
groove in the spool assembly. The pin and groove prevent the spool
from rotating when the motor turns.
This photo shows the alignment guide in the new polymeric valve
housing and the groove in the spool assembly. The guide and groove
prevent the spool from rotating when the motor turns.
Press Forward while gently against the spool to help engage it into
the valve.
Remove both old seals from the valve body by turning the seal
removal tool (#4 brass wood screw) one full turn into the seal and
pulling the seal out.
Wet the seal guide and seal with methanol (MeOH) or isopropyl
alcohol (IPA).
(IPA)
With the valve body on a smooth, hard, flat surface (bench top)
carefully insert the seal into the seal guide and press down to seat
the seal in the valve body.
Turn the valve body over and repeat for the second seal.
Being careful not to exert any side force, slide the valve body over the
seal insert and spool.
Note: The through ports on the seal body are vertical. The valve will
work if the ports are horizontal, but the fitting tubing will not reach
the ports.
IMPORTANT! Remove the seal insert from the spool. If you do not
remove the insert, the controlled valve outlet will be plugged once the
valve is re-assembled.
Remove the Teflon O-ring from the nose cap of the valve. Be careful
not to scratch the sealing surfaces.
Align the 4 mounting holes in the Nose Cap, Seal Body, and Backing
Plate to install the mounting screws.
Select Create GPV Test Method, then Press OK, the Diagnostics page
is displayed.
Press Start.
Page 2 of instructions
Follow the instructions, do not get ahead of, or fall behind the
instructions .
Press Continue.
Page 3 of instructions
Once you have removed the head and plunger assembly from the
instrument, press Continue.
Remove the check valve housing from the Primary (right pump) and
replace the check valve cartridge ( part #:WAT270941).
Be sure to insert the check valve cartridge with the arrow pointing up,
as installed in the pump manifold.
Note: The outlet (top) of the check valve has two holes.
Open the solvent conditioning system and the solvent delivery system
doors.
Loosen the captive retaining screws on the trays and pull the trays
forward. This will make it easier to work on the heads.
The 2695 now comes with Performance PLUS check valve housings.
Standard Housings
— Using a ½” open end or an adjustable wrench, hold the check valve housing and
loosen & remove the compression fitting.
— Loosen the check valve housing and remove the check valve assembly.
Pull the housing from the head and remove the check valve assembly.
Note: This is properly installed with the Check Valve extending out of
the housing.
The seal surface is on the top and bottom, the extended portion does
not effect the functionality.
This is how the assembly looks when it is removed from the pump.
The two seals at the edge of the head are the face seals
(WAT270163).
Remove the plunger seal using the plastic end of the seal removal tool
(WAT039802).
Remove the face seals using the plastic end of the seal removal tool.
Wet the face seals with methanol or IPA and insert the new face seals
into the head. There is no orientation to the face seals.
Use the edge of a credit card or ID badge to press the seal into the
head. Be careful not to scratch or leave any particles on the seals
when installing them
Wet the plunger seal, head and tool with methanol or IPA and install
the seal into the head.
Front (lower right) and back (upper left) views of the plunger wash
assembly.
The stainless steel plunger wash body and washer are re-used.
Use the plastic end of the seal removal tool to pull the plunger wash
seals from the plunger wash body.
Use a modified seal insertion tool to insert new seals in the plunger
wash body.
Wet the seals and plunger wash body with MeOH or IPA before
inserting the seals.
Wet the plunger seal, plunger wash seal, and the plunger with
methanol or IPA before re-assembling them.
Hold the plunger near the end, as shown above, to help prevent
breaking the plunger while installing it through the seals.
Page 4 of instructions
Make sure you see this page before re-inserting the head and plunger
assembly into the instrument.
Page 5 of instructions
You will return to the first Head Removal & Replacement screen.
Note: Do not get ahead or fall behind the directions on the screen.
When the instrument has exited the head removal mode, the
Diagnostics screen will be displayed.
Before performing the Static Leak Test, run the Separations Module at
4500 PSI for 5 minutes to “energize” the seals.
Remove the knurled lock nut that holds the plunger to the
syringe guide mounting bracket.
The syringe guide bracket will move down, the plunger will not move
since the knurled knob has been removed.
Note: Do not push the plunger all the way into the syringe body. Doing
so may push the end seal out of the syringe, damaging the device.
Remove the air from the syringe so the plunger tip is wet and any air
bubbles are at least 1/2 inch (1cm) above the plunger tip.
Screw the syringe into the mounting block, but do not tighten it yet.
Slide the plunger down until the threaded end is through the guide
bracket.
The syringe will move to the top position expelling the air from the
top of the syringe.
Place and tighten the knurled knob on the threaded end of the
plunger.
Press Normal States, the instrument will re-initialize all the valves.
Run a purge with compression check to verify the syringe has been
installed properly.
Main Screen
Press More.
Note:“Rename” and “Make Tpl” are blanked out (not available) Tpl
= Template
Press More.
Press More,
Press New.
Press Exit.
Note: The module is shipped with the stroke volume set at 130 μL
stroke volume does not affect flow rate
Press Next.
Press “?”
Press Exit
Press Next.
I/O screen 5 of 6
I/O screen 5 of 6
Press Next.
Screen 6 of 6 Detectors
Press Exit.
Press Yes,
Press Run.
Press OK.
Press Exit.
Press Yes.
Press Enter.
Push Yes.
Press Exit.
Open the sample compartment door and remove the carousels from
the compartment using the carousel advance function on the display
window.
After removing all the carousels, bring the carrier to the “A” position
There are two captive Allen screws (see above) that hold the
front panel in place.
Pull the bottom of the front panel forward and lower it away from the
instrument
Press down on both sides of the metal bar indicated above and slide
the carrier over the carrier drive axel.
Place the top of the front panel in place and swing the bottom in
towards the instrument.
Note: It may require some wiggling to get the front panel over the
diskette drive.
Monitoring the C/D ratio will give a good indication of the gas-to-liquid content.
This reference list shows the C/D ratio and expectations of conditions.
Definitions:
Compression - Compression value of the Primary Piston Cycle. Compression
(forward movement of the plunger) of the mobile phase to bring the Primary
operational pressure from 0psi to System operating pressure prior to
delivery stroke.
1. If the System pressure is above 650psi in the first cycle in which the C/D ratio is
greater than 1.8 and the C/D ratio remains above 1.8 for the next five consecutive
pump cycles
l (f
(for a ttotal
t l off 6 cycles).
l )
An excessive ratio value suggests that either the intake stroke has a bubble or that the
mobile phase has a higher than normal gas content.
Bubble detection is method specific. It is programmed in the Mobile Phase screen of the
method.
h d Programming off this
h alarm
l will
ll d
determine iff this
h ffunction is active.
Disable - Recovery will not be performed when a bubble is detected, the error will not
be logged.
Log Quietly -No audible or visual display alert, reports to error log only, bubble
recovery will
ill b
be automatically
t ti ll performed.
f d
Alert User - Audible alert and visual display notification box will occur, reports to error
log, bubble recovery will be automatically performed.
Stop Function - Current operation will be suspended following completion, audible
alert and visual display notification box will occur, reports to error log, recovery
sequence will not occur.
Stop Flow - Solvent delivery flow is stopped, current operation will be suspended,
audible alert and visual display
p y notification box will occur,, reports
p to error log,
g,
recovery sequence will not occur.
Note: Bubble Detection is always active. After a bubble has been detected, the alarm
setting will determine if the solvent delivery system must continue to the recovery
stage. Or if this alarm is disabled, do not continue to the recovery stage. If "Log
Quietly" or "Alert User" is programmed and a bubble continues to be detected, the
Detection/Recovery algorithm will continue indefinitely.
The C/D Ratio can be used for troubleshooting and diagnosing symptoms that
relate to a Bubble Detection error. First, y
you must ensure all the solvent
lines have been primed in the proper fashion. For a proper comparison it is
best to have all four solvent lines contain the same mobile phase. You must
also ensure proper solvent conditioning has been programmed and verify
that there is no related solvent conditioning errors logged. Once this criteria
is met this diagnostic can now be used.
After the C/D ratios for each solvent line has been recorded, the next step is to
use the C/D ratios as an indicator to help determine the cause of failure.
A solvent line with C/D ratios at 1.8 or above should be targeted to determine
failure. It can be any fluidic path component failure for those specific solvent
lines with elevated C/D ratios. Including improperly primed solvent lines,
restricted solvent sink filters, solvent tube failure, loose fittings to/from
degasser chambers, inefficient degasser chambers, and GPV leakage.
If all the solvent lines have elevated C/D ratios then the problem is most likely
do to some component in common with all the solvent lines. Targeting the
tubing connections at the output of the GPV, GPV outlet tubing connections,
primary inlet check valve, check valve housing tightness, Primary Head
Plunger Seal, Primary Head Face Seals, head and head nut tightness could
also be suspect to cause this failure.
Rather than allowing the system to drive the plungers in the “auto” mode,
bubbles may be more effectively cleared by forcing the system to operate
with a stroke length of 100 μL.
130 130u l
120 120u l
100 100u l
S t r o k e
50 50u l
Optical fence
Carousel Drive Operation Each time a carousel is in the LOAD position, the adjacent
carousel is located under the carousel dog pin shaft assembly. Just as the carrier
rotates to position and identify carousels,
The individual sample carousels must be rotated to locate specific sample vials. This is
accomplished by two stepper motors within the carousel drive mechanism and is a
multistep
lti t process. Fi
First,
t th
the sample
l carousell mustt b
be engageddb by th
the carousell
RAM shaft assembly. This is accomplished by a linear actuator motor located at the
top of the carousel drive mechanism and a dog-pin shaft at the bottom of this
mechanism. The linear actuator motor drives the entire carousel motor lift housing
and dog pin shaft down and engages the sample carousel. The dog pin shaft is so
named because of the right-angled pin at the bottom, which fits into a similarly
shaped slot in the center of each sample carousel. When the dog pin engages a
sample carousel, an optical fence, attached to the dog-pin shaft, drops into a
sensor within the carousel drive mechanism. The corresponding
p g DOG PIN LED -
DS38 on the Fluidics Driver PCB lights.
3. The optical fence may be hitting the top of the sensor bracket.
4. Insure that the adjacent cables haven’t migrated under the fence.
3. Clearing Memory
For diagnostic purposes, the 2690/5 has a set of key sequences to allow the clearing of
specific data.
Upon power up the 2690/5 will perform an initialization routine. Approximately 21
seconds after power up, the instrument will give three quick audible beeps. Then,
the Alliance logo appears.
Immediately after the three audible beeps are heard, press the appropriate Clear key
combination
bi ti sequentially.
ti ll A clear
l message will
ill th
then b
be di
displayed.
l d
Note: Softkey1 is the leftmost softkey under the main menu, softkey2 is to the right
of 1 , and so forth.
Caution: Use these codes with caution
caution. It is impossible to recover cleared data.
data
1. Errors 1 and 2 are both communications errors. Power down and check for
loose cables and connectors.
2. To correct error 3, open up the temperature window. The value may be set
from 1 to 20 degrees C.
5. Compression
i check
h k ffailed
il d
The default compression parameters are 300 psi and 20 l. If the system can
not achieve the 300 psi using 20 l of volume or less this error is displayed.
Things that could cause this error are:
1. Undegassed solvent
2 Leaking
2. L ki syringe
i plunger
l
3. Leaking V3
6 Compression
6. C i leak
l k test
t t failed
f il d
This error is displayed after the system passes the compression check. The
second half of the check is monitoring the pressure decay once the system
arrived at pressure. The default value is wait 1 minute and the pressure
shouldn’t have fallen more than 50 psi. Causes for failure:
1 Undegassed solvent
1.
3. Leaking V3
4. Too much time after the system reaches pressure (.1 to 5 minutes)
and/or too small a pressure decay allowed (10 to 100 psi)
5. A leak
l k in the
h sample
l management system
On the older type 2690/5’s (pre 11/98) the pressure in the degassing
chambers had to get below –9.8 psig and maintain an acceptable leak
rate in the “normal” configuration. In the newer 2690/5’s (after
3/99) the systems use absolute pressure and these systems must
drop below 4 psi.
Vacuum pump
PCB
Bleed valve
Vacuum chambers
3. If the PCB is OK check all of the tubing and the vacuum pump and bleed
valve. One way to check this combination is to jump out the valve and
simply check the pump and PCB combination.
combination
The new chambers are effective up to 5 ml/min where the older design are
effective up to 2 ml/min. When priming the degasser chambers, you should
flush with a minimum of 5 volumes of solvent. The new chambers with .024
ID tubing, now have an internal volume of 10 mL’s. This means that there
should be a minimum of 50 mL’s of solvent flushed through each channel of
the degasser.
Good laboratory practice is important. If buffers are in use, always remove the
buffers if the instrument is not going to be used for extended time periods.
Replace the buffers with filtered water, then follow with an aqueous/organic
mix.
Do not allow the degasser to operate for extended periods of time when the
flow rate is at 0 ml. Organic
g solvents will super
p degas,
g , resultingg in solvent
vapor condensing outside the tubular membranes.
Always "Wet Prime" after an overnight shut down or any long periods of non-
use. Program a low flow rate (0.1-0.5) for 10 minutes after the Wet Prime
to allow degassed solvent to flow into the system.
When connecting the threaded tubes to the chamber, do not overtighten the
fitting The fittings should be finger tight only
fitting. only. Be sure to use the Tefzel
(purple) ferrule. This ferrule is softer and seals with less force. Less force
is used to eliminate the need for overtightening and the possibility of
cracked fittings or chambers.
The new style Chambers are fully backward compatible for replacement into
instruments with the original style chambers. It is acceptable to have a
combination of old and new style chambers in the same 2690/5 or stand
alone vacuum degasser.
degasser If it becomes necessary to replace a chamber,
chamber only
the failed chamber should be replaced. It is not necessary to replace all
four. Customers should be informed that the flush volume is slightly higher.
It would be simpler to recommend that all chambers be flushed with 50
mL’s of solvent, even if there is a combination of chambers in the system.
8. Fatal Error
This error is displayed when a low level operating system program on the CPU
PCB has occurred. It will lock up the instrument and be display as "Fatal
Error in VRTX In addition to the previous Tip & Trick Technique dated
1/16/97 consider the full list of ideas.
Use this list at your discretion, since some ideas are more severe than others.
9 Lost Prime
9.
The 2690/5 has an internal diagnostic that monitors the system transducer
backpressure to protect the system from running dry. The diagnostic is
totally independent of the bubble detection and clearing algorithm. This
diagnostic is called the Loss Of Prime Shutdown (LOPS). If this occurs the
2690/5 will display a “Loss off of Prime” error message.
The LOPS diagnostic monitors the system backpressure of each delivery cycle.
The flow delivery will automatically be given a stop flow command, and shut
down if the instrument detects a low pressure limit threshold of 25 psi or
below for 50 consecutive (primary/accumulator) delivery cycles.
Alarm Response Description
Stop Flow Solvent delivery flow is stopped
Current operation will be suspended
Audible alert and visual dialog
notification box
Error will be logged
LOPS is always active through all flow rates for the normal flow mode. LOPS is
not active during any priming sequence.
With use of clear or translucent vial caps the alarm message "missing
vial" may occur.
3. Cut 1/4” from the end of the cable using the cutter provided with the spare
fiber optic cable or, if a cutter is not available, use a brand new razor blade.
Note: A clean cut is necessary for proper light transfer. The cable cutter
allows you to make four cuts. Do not reuse the same position in the
cable cutter.
4. Blow out the cable holder with house air to remove any dust or debris
before inserting cable.
5. Bottom out new or cleanly cut fiber optic cable in the holder and secure in
place with silicone.
When the instrument is continually giving Missing Vial errors, the vial sensor
require adjustment for greater sensitivity. This adjustment is made as
follows:
1. Go to Sensor Page.
2. Observe the two LEDs on the encoder box on the Fluidics PCB.
a. The Green LED should be blinking continuously.
b. The Yellow LED should blink when it sees light and go OFF whenever the light beam is
broken.
3. Remove the carousel and place your hand in the sample compartment to
break the light path.
4. Increase sensor sensitivity by turning the pot slowly counter-clockwise until
the Yellow LED begins to blink.
The injector sensor (top or bottom) did not see an appropriate change in state
when expected.
Check motor function
Check sensors (flag with a business card)
The most common report is after a long period of non-use (also if a buffer has
been used in the needle wash).. The dry injector seals grab the needle.
Washing out the needle wash frits with clean water or repowering the
system will cure the problem
A pressure exceeding 350 psig was detected in the sample loop when V2 was
open. The system has automatically stopped solvent delivery to prevent
damage to the syringe. This can be caused by:
Blockage in the waste line
Bad V1, V2, or V3
1 and 2 are both communications errors. Power down the system and check all
of the heater/chiller cables and connectors.
3 h/w is over temp. Try opening up the temperature range window.
4 Software monitors the amperage to the motors. If amperage goes high, the
motor is defective and the system shuts down the device.
5 There are three external motors on the H/C. If amperage goes high on at
least two of the motors you get the code and the system shuts down the
device.
6 & 7 possible bad thermistor RTD probe.
14. Seal pack geometry fault (1=seal too high, 2=seal too low, 3=seal
too wide, 4=seal too narrow)
Technical Discussion: This error occurs after a seal pack adjustment has
been performed an result parameters fall outside of acceptable range limits.
Top 30 – 80 40 +/- 10
Width 50 – 120 80 +/- 10
Bottom 80 – 200 Top + Width
Testing: Perform the Adjusting the seal procedure in the operator’s manual.
I
Insure that:
th t
5. Check the Sensor bracket for proper adjustment. The gap between the bottom of the top of the
sensor bracket and the injector motor baseplate should be approximately 5/16 of an inch. It may be
necessary to adjust the bracket UP.
To Adjust:
1. Loosen the holding screw at the base of the bracket.
2. Turn the adjusting screw on top of the bracket ¼ turn counter-clockwise.
Note: Each ¼ turn of the screw is equal to approximately 5 motor steps.
3. Re-tighten the holding screw.
Troubleshooting
Pressure on transducers - Ensure pressure is properly vented, open vent valve, re-initialize. If
transducer fault occurs, swap connections on CPU to determine cause of failure due to bad
transducer or CPU PCB.
Power Supply - Check voltages during initialization, if possible check with all
p
options "ON" after initialization to ensure full p
power draw. Check directly
y on
the power cable to the Piston Drive PCB, orange wire=24VDC, red
wire=5VDC, black wire=common, green=ground.
Piston Drive - A mechanical tolerance may be the cause. When initializing, the
plunger is gently driven to touch the head, motor steps are then counted to
determine the home position and travel of the piston. A mechanical
tolerance may cause the plunger to bounce back when it touches the head
th
therefore
f falsely
f l l causing
i th
the motor
t hhoming
i tto b
become iinaccurate.
t TTo
determine this theory, loosening the head nut before initialization will
eliminate bounce back.
Ensure that the motor is mounted securely to the piston cylinder by checking
the mounting screws for a tight fit.
YES,
DOES THE FAILURE OCCURE AT LOW FLOW RATE AND LOW
PRESSURES? Follow troubleshooting ideas listed above.
YES,
DOES THE FAILURE OCCUR AT HIGH FLOW RATE AND HIGH PRESSURE,
DURING WET OR DRY PRIMINGPRIMING, OR DURING BUBBLE DETECTION
AND CLEARING?
The piston driving the plunger into the head, due to mechanical problems could
cause this. Also, could be caused by torque problems with the motor or a
voltage inconsistency or lack of proper voltage from the power supply, bad
connections or pin contacts. Note all voltages should be checked with all
options "ON" under full load.
Are all 3 transducers the same? Yes, all the transducers are common in style.
The replacement part can be ordered using WAT270966.
The most common failure will be identified upon initialization. If the software
can not electronically zero the transducer an "Out of range" error will occur.
When electronically zeroing there is a specified window of zeroing range, if
outside the range an error will occur. This range can be viewed in the
Service Utilities page, a box containing all the voltage offsets will be
displayed. A perfect transducer will have a reading of 0.5V.
Bubble found on intake Wet prime and allow system to sit at zero flow
with the degasser
g in continuous mode for approx.
pp
10 min. prior to starting the run. If the flow
rate is greater than 1 mL/min. install extended
flow inlet tube.
Compression check failed Solvent not degassed properly. Check for bubble
on syringe plunger. Test seal pack and valve 3.
Perform appropriate maintenance.
Solvent delivery h/w fault Cycle power. If message persists call Waters Service
Representative.
System over pressure Locate restriction... guard column / column / inline filter /
blocked tubing.
System under pressure Connect column. Locate leak. 50 psi is needed to run the
system.
Chromatographic Errors
Chromatographic Errors
Baseline noise
Flow fluctuations Malfunctioning check valve. Leaks,
Seals, fittings.
Chromatographic Errors
Old L
Lamp R l
Replace llamp
3 Perform a dry prime until solvent flows from each of the four lines.
3. lines
7 Ch
7. Checkk the
th Pressure
P Ripple
Ri l on page twot off the
th Status
St t Page.
P Th
The
ripple should be less than 2% of the system pressure. (For systems
pressures above 1000 psi, repeat Wet Prime if the ripple is too high)
9. Run samples.
* The degasser should never be left on with no flow for long periods of
time (I.e. overnight)
2. For each line that will be used, starting with the organic solvent, perform a
1.5 minute Wet Prime at a flow rate of 5.0 mL/min. **
3. Once all lines are primed, set flow rate to 0mL/min with degasser on
continuous for 10 minutes.
4. Start the flow with the initial conditions of your chromatographic method.
Equilibrate with 10 column volumes of solvent.
5. Check the Pressure Ripple on page two of the Status Page. The ripple
should be less than 2% of the system pressure. (for system pressures
above 1000psi, repeat Wet Prime if the ripple is too high)
7. Run Samples.
* The degasser should never be left on with no flow for long periods of time.
(i.e. overnight)
* The degasser should never be left on with no flow for long periods of time.
(i.e. overnight)
Note: When first starting to equilibrate your column, one or two Bubble
Detects is normal
normal. The system is detecting any small bubbles and using its
its’
automatic algorithm to clear the bubbles.
Note: The volume of each solvent line in a 2695 including the vacuum
degasser chamber is 5.5 mL. This volume must be considered when priming
the system and changing to a different mobile phase.
Note: The degasser can be turned off, the flow rate stopped and the lamp
turned off (if controlling the detector through the Alliance) in a Shutdown
Method.
1. Press the Diag screen key in the Main Menu screen. The Diagnostic
screen appears.
2. Attach the syringe adapter (from the Start-up Kit) to the syringe.
3. Remove the solvent filter from the plunger seal wash inlet or remove
the plunger seal wash waste line from the waste container. (Either
pushing solvent through it or drawing solvent through it can be used to
prime the plunger seal wash pump)
4 Fill the s
4. syringe
inge with
ith pl
plunger
nge seal wash
ash solvent*
sol ent* and attach the syringe
s inge
adapter to the end of the plunger seal wash line.
5. Press the Prime Sealwash Screen key and then press the Start screen
key. The plunger seal wash procedure begins. Push on the syringe to
push seal wash solution through the system or draw on the syringe to
pull
ll solvent
l t th
through
h th
the plunger
l seall wash
h pump.
6. When solvent flows out of the plunger seal wash line or into the syringe
(the pump will run quieter when it is primed) press the Halt screen key.
7 Reinstall the solvent filter and place the seal wash inlet line back into
7.
the plunger seal wash reservoir or place the plunger seal wash waste
line back into the waste container. To finish the priming, press the Start
screen key again.
* Choose a Seal Wash solution that has high solubility with salts and /or
buffers used in the mobile phase. (Usually a solution of 80 - 90%
aqueous, 20 - 10% methanol)
1. Make sure Needle Wash line (Green Line) is in its appropriate bottle.
2. Press the Diag screen key in the Main screen. The Diagnostics screen
appears.
3. Press the Prime NdlWsh screen key. The 30-second needle wash
procedure begins. If solvent does not flow out of the waste line (the
yellow line) press the Start Again screen key.
Note: Choose a Needle Wash solution that your sample is soluble in.
B ffe ed solutions
Buffered sol tions or
o a mobile phase modifier
modifie such
s ch as TFA are
a e not
recommended. If these solutions are necessary they must be flushed
from the instrument.
Plunger WAT270488 2
Syringe WAT073109 1
WAT025313 1
* Screw, Compression
*Ferrule 410001125 1
*Needle 405001338 1
*Needle
Needle Wash Filter 415000230 1
The 2695 PM kit contains all the parts subject to normal wear to be
p
replaced in one instrument.
It comes with a 7 language protocol outlining instrument diagnostic
tests, accuracy calibration, and tables for recording results.
Items marked with an asterisk (*) are also found in the Seal Pack
Rebuild Kit,
P/N
/ WAT271019.
The kit contains one Seal Wash Tube Seal, P/N WAT270668.