user manual

chromatography software

User Reference Manual

um 03-0014-90
UNICORN 5.0 User Reference Manual
03-0014-90/91 Edition AB
2004-02

Office addresses:
Amersham Biosciences AB
SE-751 84 Uppsala
Sweden

Amersham Biosciences UK Limited

Amersham Place
Little Chalfont
Buckinghamshire
England HP7 9NA

Amersham Biosciences Corp.

800 Centennial Avenue
P.O. Box 1327
Piscataway NJ 08855
USA

Amersham Biosciences Europe GmbH

Munzinger Strasse 9
D-79111 Freiburg
Germany

Amersham Biosciences K.K.

Sanken Building
3-25-1 Hyakunincho, Shinjuku-ku
Tokyo 169-0073
Japan

Amersham Biosciences China Limited

13/F., Tower I
Ever Gain Plaza
88 Container Port Road
Kwai Chung, New Territories
Hong Kong

www.amershambiosciences.com

Trademarks:
UNICORN, Drop Design, FPLCdirector, OligoPilot, BioProcess, Ettan, ÄKTA, ÄKTAxpress, ÄKTAbasic,
ÄKTAexplorer, ÄKTAFPLC, ÄKTApilot and ÄKTApurifier are trademarks of Amersham Biosciences Limited.
Amersham and Amersham Biosciences are trademarks of Amersham plc.

Adobe, Acrobat and Distiller are trademarks of Adobe Systems Inc.

Microsoft and Windows are trademarks of the Microsoft Corporation in the United States and/or other countries.

Terms and Condition of Sale:

Unless otherwise agreed in writing, all goods and services are sold subject to the terms and conditions of the
company within the Amersham Biosciences group which supplies them. A copy of these terms and conditions is
available on request. Any use of this software is subject to Amersham Biosciences Standard Software End-User
License Agreement.

© Copyright Amersham Biosciences AB 2004 - All rights reserved.

 Table of Contents

Table of Contents
1. Introducing UNICORN..........................................................................................................9
1.1. About UNICORN.............................................................................................10
1.2. About this manual...........................................................................................14
1.3. About the UNICORN user documentation..........................................................17
2. UNICORN concepts............................................................................................................20
2.1. Concept definitions.........................................................................................21
2.2. The UNICORN user interface........................................................................................24
2.2.1. UNICORN Manager....................................................................................25
2.2.2. The Method Editor module.........................................................................28
2.2.3. The System Control module........................................................................32
2.2.4. The Evaluation module...............................................................................35
2.2.5. Search functions.......................................................................................37
2.2.6. Help functions and manuals.......................................................................39
2.2.7. Snapshots.................................................................................................41
2.3. Quick Start Guide............................................................................................44
3. General system operations.................................................................................................45
3.1. Log on routines and log off routines..................................................................46
3.2. How to create a new user.................................................................................50
3.3. How to assign user properties...........................................................................53
3.4. How to change your passwords and user attributes..............................................56
3.5. How to connect to the chromatography system..................................................58
3.6. How to back up and restore system data............................................................63
3.7. How to set up a printer....................................................................................66
4. Files and folders in UNICORN.............................................................................................67
4.1. How to create folders.......................................................................................68
4.2. How to open and preview files..........................................................................69
4.3. How to arrange and locate your files..................................................................72
4.4. How to copy, delete, rename and backup files and folders...................................75
5. How to create a method.....................................................................................................79
5.1. How to use the Method Wizard.........................................................................80
5.2. How to use the Method templates.....................................................................85
5.3. How to use Text instructions.............................................................................88
5.4. How to sign the method...................................................................................91
6. How to edit methods..........................................................................................................92
6.1. The Method Editor interface.........................................................................................93

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6.1.1. Method Editor module................................................................................94

6.1.2. Text Instructions editor..............................................................................95
6.2. Method blocks............................................................................................................97
6.2.1. How to view method blocks........................................................................98
6.2.2. How to call method blocks........................................................................100
6.2.3. How to add method blocks.......................................................................101
6.2.4. How to delete method blocks ...................................................................104
6.2.5. How to rename method blocks..................................................................106
6.2.6. How to find, copy and move method blocks................................................107
6.2.7. How to import method blocks...................................................................109
6.3. Method instructions..................................................................................................111
6.3.1. How to read method instructions...............................................................112
6.3.2. How to add method instructions................................................................113
6.3.3. How to delete method instructions............................................................114
6.3.4. How to change or move method instructions...............................................115
6.4. How to use method variables..........................................................................118
6.5. Run Setup.................................................................................................................122
6.5.1. Overview of Run Setup.............................................................................123
6.5.2. The Variables tab.....................................................................................125
6.5.3. The Scouting tab.....................................................................................128
6.5.4. The Questions tab....................................................................................129
6.5.5. The Gradient tab.....................................................................................133
6.5.6. The Notes tab.........................................................................................135
6.5.7. The Evaluation Procedures tab..................................................................136
6.5.8. The Reference Curves tab.........................................................................140
6.5.9. The Columns tab.....................................................................................142
6.5.10. The BufferPrep tab................................................................................143
6.5.11. The Method Information tab...................................................................146
6.5.12. The Result Name tab.............................................................................147
6.5.13. The Frac-950 tab .................................................................................149
6.5.14. The Start Protocol tab............................................................................151
6.5.15. How to export the values in the Run Setup...............................................153
6.6. How to use selected method instructions....................................................................154
6.6.1. Base instruction......................................................................................155
6.6.2. Instructions at the same breakpoint...........................................................157
6.6.3. Block and method length.........................................................................158

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6.6.4. Messages and Set_Marks..........................................................................160

6.6.5. How to delay a method.............................................................................162
6.6.6. Linear flow rates......................................................................................163
6.6.7. Gradients and eluent concentrations..........................................................164
6.7. Standard Watch conditions.............................................................................166
6.8. How to save or delete a method template.........................................................171
6.9. How to print a method...................................................................................172
6.10. How to export a method...............................................................................174
7. Scouting.........................................................................................................................175
7.1. How to set up a Scouting Scheme...................................................................176
7.2. How to define different columns for scouting...................................................181
8. MethodQueues................................................................................................................182
8.1. How to create a new MethodQueue.................................................................183
8.2. How to edit a MethodQueue...........................................................................187
9. How to perform method runs............................................................................................189
9.1. How to start a method run..............................................................................190
9.2. How to monitor a method run.....................................................................................193
9.2.1. How to customize System Control panes....................................................194
9.2.2. The Run Data pane..................................................................................196
9.2.3. The Curves pane......................................................................................199
9.2.4. The Flow Scheme pane............................................................................204
9.2.5. The Logbook pane...................................................................................205
9.3. Manual system control..............................................................................................207
9.3.1. The toolbar and status bar........................................................................208
9.3.2. Manual instructions.................................................................................212
9.3.3. Alarms and warnings................................................................................215
9.4. How to perform a scouting run........................................................................216
9.5. How to perform a MethodQueue run................................................................217
9.6. If the network connection fails........................................................................219
10. How to view results.......................................................................................................220
10.1. How to open a result file..............................................................................221
10.2. How to use the File Navigator.......................................................................222
10.3. Basic presentation of chromatograms.......................................................................226
10.3.1. Introduction and temporary chromatograms..............................................227
10.3.2. The chromatogram window.....................................................................229
10.4. How to optimize the presentation of a chromatogram................................................234

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 Table of Contents

10.4.1. How to make changes in the Chromatogram Layout dialog box...................235

10.4.2. The Curve tab and Curve Names tab........................................................236
10.4.3. The Curve Style and Color tab.................................................................238
10.4.4. How to change and fix the axes...............................................................240
10.4.5. How to save and apply a layout...............................................................242
10.4.6. How to show part of a curve....................................................................244
10.4.7. How to change the size of Fraction, Injection and Logbook marks...............246
10.5. How to print active chromatograms...............................................................247
10.6. How to create and print reports................................................................................249
10.6.1. How to create and print a customized report.............................................250
10.6.2. How to create and print a standard report................................................264
10.6.3. How to edit an existing report format.......................................................267
10.7. Run documentation.....................................................................................270
11. How to edit results........................................................................................................273
11.1. How to reduce noise and remove ghost peaks.................................................274
11.2. How to subtract a blank run curve.................................................................275
11.3. How to add curves.......................................................................................277
11.4. How to enter and edit text in the chromatogram.............................................278
11.5. How to pool fractions...................................................................................279
11.6. How to match protein activity to a curve........................................................285
11.7. How to rename chromatograms, curves and peak tables..................................286
11.8. How to import and compare different runs................................................................287
11.8.1. How to use the Multifile Peak Compare wizard.........................................288
11.8.2. How to import and compare chromatograms.............................................302
11.8.3. How to import and compare curves..........................................................305
11.8.4. How to stack and stretch curves..............................................................312
11.8.5. How to produce a mirror image...............................................................316
11.9. How to import and export results..............................................................................318
11.9.1. How to import results.............................................................................319
11.9.2. How to export results.............................................................................321
11.10. How to sign results electronically................................................................325
11.11. How to save results and exit the Evaluation module......................................326
12. Evaluation....................................................................................................................327
12.1. Peak integration.....................................................................................................328
12.1.1. Baseline calculation...............................................................................329
12.1.2. How to perform a peak integration...........................................................330

03-0014-90 • p iv
 Table of Contents

12.1.3. How to optimize the baseline with a morphological algorithm.....................336

12.1.4. How to optimize the baseline with a classic algorithm...............................340
12.1.5. How to edit the baseline manually...........................................................348
12.1.6. How to edit the peaks............................................................................351
12.1.7. How to integrate part of a curve and how to exclude or skim peaks.............358
12.1.8. Measurements.......................................................................................363
12.2. Other evaluations....................................................................................................365
12.2.1. Peak purity and peak identification.........................................................366
12.2.2. How to find slope values........................................................................369
12.2.3. How to simulate a peak fractionation.......................................................372
12.2.4. How to create curves..............................................................................373
12.2.5. How to use the Fraction Histogram..........................................................377
12.3. Automated evaluation procedures............................................................................378
12.3.1. How to create a new procedure...............................................................379
12.3.2. How to edit a procedure.........................................................................382
12.3.3. How to run a procedure..........................................................................384
12.3.4. How to rename and remove procedures....................................................387
13. The Analysis module.....................................................................................................388
13.1. General information about the module...........................................................389
13.2. Quantitation overview..............................................................................................393
13.2.1. General information about quantitation....................................................394
13.2.2. External standard quantitation................................................................397
13.2.3. Internal standard quantitation.................................................................400
13.2.4. Standard addition quantitation................................................................404
13.2.5. Recovery calculation..............................................................................406
13.2.6. General reliability factors for the quantitation techniques..........................408
13.3. How to prepare for quantitation................................................................................409
13.3.1. Preparations before quantitation.............................................................410
13.3.2. How to create a quantitation table...........................................................411
13.3.3. How to edit and update a quantitation table.............................................422
13.4. How to quantitate the sample...................................................................................428
13.4.1. External and internal standard quantitation..............................................429
13.4.2. Standard addition quantitation................................................................432
13.4.3. How to calculate the recovery factor........................................................435
13.5. Automated quantitation............................................................................................438
13.5.1. How to set up for automated quantitation................................................439

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 Table of Contents

13.5.2. How to perform automated quantitation...................................................441

13.5.3. How to perform automated update..........................................................442
13.6. How to measure molecular size...............................................................................448
13.6.1. Overview of molecular size determination.................................................449
13.6.2. How to determine the molecular size.......................................................451
14. System settings.............................................................................................................458
14.1. General information about system settings.....................................................459
14.2. Alarms.......................................................................................................461
14.3. Curves........................................................................................................463
15. System maintenance and error reporting........................................................................464
15.1. System maintenance functions.....................................................................465
15.2. How to generate problem reports.............................................................................469
15.2.1. How to generate a report from the UNICORN Manager..............................470
15.2.2. How to generate a report from the System Control.....................................473
A. Troubleshooting..............................................................................................................476
A.1. Logon...........................................................................................................477
A.2. UNICORN access..........................................................................................479
A.3. Methods and method runs..............................................................................482
A.4. Evaluation....................................................................................................487
A.5. ÄKTAdesign system specific problems.............................................................488
B. Evaluation functions and instructions...............................................................................489
B.1. Smoothing algorithms....................................................................................490
B.2. Baseline calculation theory............................................................................493
B.3. Peak table column components......................................................................497
B.4. Procedure instructions...................................................................................504
C. Curve fit models and statistics.........................................................................................520
C.1. Curve fit models............................................................................................521
C.2. Statistics......................................................................................................525
D. The Column list...............................................................................................................527
D.1. How to edit the Column List...........................................................................528
E. How to create and edit BufferPrep recipes........................................................................536
E.1. How to create a BufferPrep recipe...................................................................537
E.2. How to edit a BufferPrep recipe......................................................................542
F. Method examples............................................................................................................545
F.1. Simple equilibration......................................................................................546
F.2. Equilibration with simple safeguard.................................................................548

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 Table of Contents

F.3. Equilibration with extra safeguard...................................................................549

F.4. Collection of absorbance peaks.......................................................................551
F.5. Collection of three absorbance peaks...............................................................553
F.6. Messages......................................................................................................555
F.7. Quality control procedure...............................................................................557

• p vii
 Table of Contents

03-0014-90 • p viii
 Introducing UNICORN 1

1 Introducing UNICORN

Introduction This chapter contains:

• A general overview of the UNICORN™ system.
• Information about the user documentation for UNICORN and how to use it.

In this chapter This chapter contains these sections.

Topic See
About UNICORN 1.1

About this manual 1.2

About the UNICORN user documentation 1.3

•p9
 1 Introducing UNICORN
1.1 About UNICORN

1.1 About UNICORN

Introduction This section is a general overview of the UNICORN system.

What is UNICORN is a complete package for control and supervision of chromatography

UNICORN? systems. It consists of control software and a controller card for interfacing the
controlling PC to the chromatography liquid handling module.
Liquid chromatography is used in separation processes, for analytical purposes or
in the biochemical process industry.
UNICORN is a trademark of Amersham Biosciences Limited.

Operating environ- UNICORN runs on a PC under Microsoft® Windows® 2000 or Microsoft

ment Windows XP. It is designed to run under English keyboard settings.
Note: Microsoft and Windows are registered trademarks of the Microsoft
Corporation in the United States and/or in other countries.

Windows func- Most Windows functions are also available in UNICORN, including
tions
• cut and paste
• right-click short-cut menus

Note: Drag and drop is not available. File and folder handling in UNICORN also
differs from the general Windows file manager standard.

Bar code reader You can connect a bar code reader to the PC and use the reader to enter information
instead of using the keyboard. This can be useful for example when entering
information like batch IDs.

Compatible chro- UNICORN can be used with a number of systems including

matography sys-
tems • ÄKTA™ design systems
• BioProcess™ systems

Note: All examples in this guide are based on an ÄKTAexplorer™ 100 system that
operates with the E100F400 strategy.If you use another system you may find that
the descriptions and instructions do not match your system on every point. In that
case you also need to refer to the user documentation for your specific
chromatography system.

03-0014-90 • p 10
 Introducing UNICORN 1

System networks UNICORN can be installed on a stand-alone computer to control only a single,
locally attached system. However, a stand-alone computer can control up to four
separate systems. In a network installation each computer workstation can operate
many systems regardless if they are locally connected or not. Each system can only
be operated by one workstation at a time, but several may view the output data.

Software modules The UNICORN control software consists of four integrated modules:

Module Function
UNICORN Manager File handling and administration, e.g.
definition of systems and user profile
etc.

Method Editor To create and edit methods for pre-

programmed control of chromato-
graphy systems.

System Control To control and monitor the separation

processes online, through method-
based or manual control.

Evaluation To evaluate and present stored results

from separation processes.

Note: All modules are active when the program is operating, and are not closed
when they are minimized. A minimized System Control unit may control a process.
All modules will normally open when the program is started. However, a user
profile may be set up so that not all modules are available. Only the available
modules will be displayed.

• p 11
 1 Introducing UNICORN
1.1 About UNICORN

Work flow The work flow in UNICORN can be divided into four distinct stages. Each stage
is described in separate chapters in this manual. The flow chart below shows the
work flow stages.

Help functions An online help utility is included in the UNICORN software. The table below
describes how to access the help utility.

If you want to access... Then...

the general help utility. open the Help menu in any of the software modules.

context-specific help • click the Help button in the dialog box

topics.
or
• press the F1 key on your keyboard.

the online manuals. open the Help menu in any of the software modules
and select Manuals.

Security The table below describes the main security functions in UNICORN:

Feature Function
Access Security Only authorized users can access
UNICORN. Each user is assigned an
access level, which defines the func-
tions that the user is permitted to use.

03-0014-90 • p 12
 Introducing UNICORN 1

Feature Function
Connection Security A running system can only be con-
trolled from one connection. Systems
may be locked with a password to
prevent other, un-authorized users
from changing parameters.

Data Security Result files from an ongoing separa-

tion run can be saved automatically at
preset intervals to minimize data loss
if the system fails. The results are
saved locally if the network communic-
ation fails.

Electronic Signatures Method and result files can be signed

electronically for enhanced security
and accountability.

• p 13
 1 Introducing UNICORN
1.2 About this manual

1.2 About this manual

Introduction This section is a general description of the manual, the contents and the
pre-requisites for the examples and instructions that are presented in the User
Reference Manual.

The purpose of The purpose of the User Reference Manual is to present a comprehensive guide to
the User Reference the UNICORN system for a user either with previous experience of this system or
Manual
from other, similar chromatography systems. The system is presented in detail,
along with practical instructions of how to operate a model system.

Systems covered This manual and the corresponding version of Getting Started with UNICORN
by this manual covers the following systems:
• ÄKTAexplorer
• ÄKTApurifier™
• ÄKTAFPLC™
• ÄKTAbasic™
• ÄKTApilot™
• Ettan™ LC

Note: Adapted versions of this manual are available for ÄKTAxpress™, ÄKTA
oligopilot™ and BioProcess systems.

The model system For practical reasons the user documentation is based on a model system that
consists of:

03-0014-90 • p 14
 Introducing UNICORN 1

• ÄKTAexplorer 100
• Strategy E100F400
• Frac-950

Note: If you use another system you may find that the descriptions and instructions
do not match your system on every point. In that case you also need to refer to the
user documentation for your specific chromatography system.

Refer to other The User Reference Manual does not contain information about the installation
manuals procedure or network configuration. You will find this information in the
Administration and Technical Manual.
Sometimes you may find it more convenient to refer to the Getting Started with
UNICORN guide for a linear, step-by-step instruction how to perform a task.
Note: When you install the UNICORN software you choose which manuals you
wish to install. You can also install the manuals after the program installation.

Document struc- The manual is divided into chapters. Each chapter starts with a brief overview that
ture presents the contents and the headings for the sections that the chapter contains.
Most sections begin with an introduction that summarizes the content. Some
sections are divided into sub-sections.
A section is divided into blocks of information with separating lines. The blocks
are identified by a label in the margin. This makes it easier for you to quickly scan
a page to find the exact topic you are looking for.

Typographical Menu commands, field names and other text items from the software are quoted
representations exactly as they appear on the screen, in a bold typeface:
Example: Run Setup
Search paths are shown in a bold typeface with a separating colon between each
level:
Example: View:Panes:Customize (i.e. the menu command Customize in the sub-menu
Panes from the View-menu).
Text entries that UNICORN generates or that the user must type is represented
by a monotype typeface:
Example: Connection change

• p 15
 1 Introducing UNICORN
1.2 About this manual

Pre-requisites The following pre-requisites must be fulfilled before you can use this manual the
way it was intended:
• You need to have a general understanding of how your PC and Windows works.
In most cases universal computer functions will not be explained.
• UNICORN must be installed and configured correctly on your computer.
• You need to understand the concepts of liquid chromatography. Terminology
and functionalities will be explained only when they differ from normal practise.
• Before you try to operate a chromatography system based on the instructions
in this manual you need to study and understand the safety information that is
part of the system documentation.

03-0014-90 • p 16
 Introducing UNICORN 1

1.3 About the UNICORN user documentation

Introduction The user documentation for UNICORN is divided into three separate manuals.
This section is an overview of the contents and the relationship between the
manuals.

The manuals The three manuals are:

• Getting Started with UNICORN
• UNICORN User Reference Manual (See 1.2 About this manual on page 14).
• UNICORN Administration and Technical Manual

User info about The questions and answers in the table below describe the features of the Getting
Getting Started Started manual.

Question Answer
Who should read Getting Started? Users that are new to the UNICORN
system and with limited experience
from other chromatography systems.

What do I need before I start? A basic knowledge of PC and

Windows functions and an understand-
ing of the concepts and terminology
of liquid chromatography.

What are the contents of Getting Basic descriptions of UNICORN and

Started? its use, based on a model system.

How should I use Getting Started? Read in front of your computer and
test the instructions at the same time.

User info about The questions and answers in the table below describes the features of the User
the User Reference Reference Manual.
Manual

Question Answer
Who should read the User Reference • Users that are experienced with
Manual? previous UNICORN system ver-
sions.
• Users with vast experience from
other chromatography systems.

• p 17
 1 Introducing UNICORN
1.3 About the UNICORN user documentation

Question Answer
What do I need before I start? Knowledge of PC and Windows func-
tions and an understanding of the
concepts and terminology of liquid
chromatography. Preferably previous
experience with UNICORN.

What are the contents of the User • Detailed descriptions of

Reference Manual? UNICORN.
• Instructions on how to use the sys-
tem, with suggested alternatives.

Most instructions are based on a

model system.

How should I use the User Reference Depending on your previous experi-
Manual? ence you can either read whole
chapters from the beginning to the
end, or only selected sections for refer-
ence.

User info about The questions and answers in the table below describes the features of the
The Administra- Administration and Technical Manual.
tion and Technical
Manual
Question Answer
Who should read the Administration System administrators.
and Technical Manual?

What do I need before I start? • General knowledge of UNICORN.

• Knowledge of PC, Windows and
general network administration
functions.
• An understanding of the concepts
and terminology of liquid chroma-
tography.

What are the contents of the Adminis- Detailed instructions of:

tration and Technical Manual? • How to install and maintain
UNICORN in a network environ-
ment.
• How to create and administrate
user profiles.

Most instructions are based on a

model system.

03-0014-90 • p 18
 Introducing UNICORN 1

Question Answer
How should I use the Administration • If you are an experienced adminis-
and Technical Manual? trator of previous UNICORN ver-
sions you can read selected sections
for reference.
• If this is your first experience of
UNICORN we recommend that
you study the manual in detail.

• p 19
 2 UNICORN concepts

2 UNICORN concepts

Introduction This chapter contains:

• Definitions and descriptions of some of the specific concepts that are presented
in this manual and in other UNICORN manuals.
• An overview of the UNICORN user interface.
• A Quick Start Guide that can be used as a shortcut for experienced users that
want to start right away.

Note: General concepts and common chromatography terminology are not

explained here.

In this chapter This chapter contains these sections.

Topic See
Concept definitions 2.1

The UNICORN user interface 2.2

Quick Start Guide 2.3

03-0014-90 • p 20
 UNICORN concepts 2

2.1 Concept definitions

Introduction This chapter contains explanations and definitions of a number of UNICORN

concepts that are used in this manual.
The concepts are organized in alphabetical order.

Alarms Systems settings or method instructions specify acceptable limits for monitor signals
during a separation run. An Alarm dialog box will be displayed on the screen if the
monitored values exceed or fall below specified limits. The system will be paused.

Batch run You can perform a Batch run of a number of result files in the Evaluation module.
The files do not have to be open and the run operates in the background. The
procedure is useful if you want to print a number of results with the same settings,
or if you want to perform integration with the same parameter settings on many
results.

BufferPrep BufferPrep is a function to prepare a buffer of different pH and salt concentrations

online from four stock solutions. This eliminates the need to manually prepare
new buffers every time the pH needs to be changed.
Note: BufferPrep is only available for some ÄKTAdesign systems.

Chromatogram A chromatogram is a collection of data represented by a number of curves that

have been created during a separation run, including UV, conductivity, pH, fraction
marks etc. The original raw data curves cannot be deleted or modified. They can
be used as a basis for evaluation procedures and subsequent creation of new curves.
A chromatogram can also contain curves that have been created and saved during
an evaluation session.

Curves The monitor signals from the chromatography run are displayed graphically as
curves.

Method The program instructions for a chromatography run are defined in a Method. A
Method can be divided into blocks that represent steps in the separation process.
Each block consists of a series of instructions that request specific operations in
the system.

MethodQueue MethodQueues are used to link several methods together, on the same or on different
systems.
Example: A MethodQueue can be set up to conduct a CIP study of a number of
columns, through a controlled series of scouting runs.

• p 21
 2 UNICORN concepts
2.1 Concept definitions

Method Wizard The Method Wizard is a user-friendly tool to create new methods. The Wizard takes
the user step-by-step through the creation process.
Method Wizards are supplied with UNICORN installations for ÄKTAdesign systems.

Result files UNICORN creates Result files when a method is run. The Result files contain:
• Run data from the monitors in the chromatography system.

Example: UV absorbance, flow rate, conductivity etc.

• Documentation from the run.

Example: Logbook entries, calibration settings, scouting parameters, text method

etc.
• Saved results from evaluations of the run data.

Example: Peak integrations, simulated peak fractionations etc.

Scouting Scouting is used to repeat a series of Method runs automatically with predetermined
changes in the values for one or more Variables. A Scouting Scheme is defined as
part of the method.
Scouting is used for optimizing chromatographic processes.

Strategy Part of the UNICORN software is specific for the system that it is set up to operate.
The system specific part is usually referred to as the Strategy. The Strategy defines
available method and manual instructions, system settings, run data, curves and
Method Wizards.

Note: The examples in this guide are generally based on the E100F400 strategy.

Template Templates are basic methods that can be used as a starting point for developing
customized methods. The method variables in a suitable Template is adjusted to
create a method for another application.

Variable Instruction parameters and values at breakpoints in the Methodmay be defined as

Variables. Variables makes it easy to adapt a method to a particular chromatography
run.
• A framework Method with default parameters can be changed to create variants.
• A Method can be used in automatic Method Scouting, where one or more
parameter Variables are changed systematically.

03-0014-90 • p 22
 UNICORN concepts 2

Warnings Systems settings or method instructions specify acceptable limits for monitor signals
during a separation run. A Warning dialog box may be displayed on the screen if
a specified limit is exceeded. The system will still continue to run after a Warning.

• p 23
 2 UNICORN concepts
2.2 The UNICORN user interface

2.2 The UNICORN user interface

Introduction This section is an overview of the four UNICORN modules with descriptions of
some of the elements of the user interface. The section also contains a description
of the search functions in UNICORN.
Note: A user profile can be set up so that the user only has limited access to the
modules described in this chapter. Only the available modules will open when the
program is started.

In this section This section contains these sub-sections.

Topic See
UNICORN Manager 2.2.1

The Method Editor module 2.2.2

The System Control module 2.2.3

The Evaluation module 2.2.4

Search functions 2.2.5

Help functions and manuals 2.2.6

Snapshots 2.2.7

03-0014-90 • p 24
 UNICORN concepts 2

2.2.1 UNICORN Manager

Introduction The UNICORN Manager is mainly used for file and folder administration.

The UNICORN The module is divided into two windows, the Methods window and the Results
Manager windows window. See the illustration below:

The Methods win- The Methods window contains all the saved methods, MethodQueues and all the
dow folders containing methods that are available to the user. See the illustration below:

Note: The icons for MethodQueue folders are different from the regular folder icon.

• p 25
 2 UNICORN concepts
2.2 The UNICORN user interface
2.2.1 UNICORN Manager

The Results win- The Results window contains all the saved results and all the result folders.
dow

Note: The icons for Scouting folders are different from the regular folder icon.

Toolbar icons in The table below describes the toolbar icons in the module.
the UNICORN
Manager
Icon Function
The Logon/Logoff icon is used to log on or log off the system.
Note: The arrow in the Logoff icon points away from the door.

The Instant Run icon immediately starts a run from a selected template
or from a wizard.

The New Method icon opens the Method Editor module and displays
the New Method dialog box.

The System Control icon activates the first connected System Control
module and displays the Manual instruction dialog box.

The Evaluation icon opens the Open Result dialog box. Select a result
file and click OK to start the Evaluation module.

The MethodQueue icon opens the MethodQueue Editor.

The Existing MethodQueue icon opens the Running MethodQueue dialog

box to display MethodQueues in progress.

03-0014-90 • p 26
 UNICORN concepts 2

Limited access to Some user groups may be defined to have only a limited access to the UNICORN
the UNICORN Manager functions. The available functions in the limited version are:
Manager
• Log off
• Change User Attributes
• Change Password
• Quit Program
• Help

There is also a Cancel button which minimizes the dialog box. The illustration
below shows the limited access version of the UNICORN Manager.

Note: For more information about how to change passwords and user attributes
please refer to 3.4 How to change your passwords and user attributes on page 56.
For more information about how to log off and quit the program, please refer to
3.1 Log on routines and log off routines on page 46.

• p 27
 2 UNICORN concepts
2.2 The UNICORN user interface
2.2.2 The Method Editor module

2.2.2 The Method Editor module

Introduction The Method Editor module provides complete facilities for advanced editing of the
methods.

Two modes The Method Editor interface operates in two modes:

• Run Setup
• Text Instructions

Run Setup Run Setup is a dialog box with a number of tabs that define the method properties.
See the illustration below:

03-0014-90 • p 28
 UNICORN concepts 2

Text Instructions Text Instructions are used for advanced editing. Up to five different display panes
can be open at the same time:
• The Block pane.
• The Flow Scheme pane.
• The Gradient pane.
• The Text pane.
• The Instruction box pane.

See the illustration below:

The Block pane The Block pane contains a graphical representation of the method organized in
blocks. See the illustration below:

• p 29
 2 UNICORN concepts
2.2 The UNICORN user interface
2.2.2 The Method Editor module

The Flow Scheme The Flow Scheme pane displays the configuration of the system components. The
pane pane is static and for information only. See the illustration below:

The Gradient The Gradient pane provides a graphical overview of the block structure and eluent
pane gradient in the current method. See the illustration below:

The Text pane The Text pane displays the method as a list of text instructions. The instructions
can be organized in blocks, denoted by blue square symbols. The blocks can be
expanded to show the instructions within the block. See the illustration below:

03-0014-90 • p 30
 UNICORN concepts 2

The Instruction The Instruction box pane is used to enter, edit or delete instructions. See the
box pane illustration below:

Toolbar icons in The table below describes the toolbar icons in the module.
the Method Editor
Icon Function
The New icon opens the New Method dialog box. The dialog box is
used to create a new method.

The New Block icon opens the New Block dialog box, which is used
to add blocks to a method.

The Open icon displays all available method files and method folders
in the Open dialog box.

The Save Method icon saves the edited method.

The Print icon opens the Print dialog box. Select the method elements
that you want to print.

The Customise Panes icon opens the Customise Panes dialog box,
which is used to select the panes that are open in Text Instructions
mode.

The Text Instructions icon opens the Method Editor in Text Instructions
mode.

The Run Setup icon opens the Method Editor in Run Setup mode.

The Log Format icon opens the Log Format dialog box, which is used
to display the accumulated time or volume for a method.

The Method Wizard icon opens the Method Wizard, which is used to
create new methods.

• p 31
 2 UNICORN concepts
2.2 The UNICORN user interface
2.2.3 The System Control module

2.2.3 The System Control module

Introduction The System Control module is used to perform and monitor separation runs.

The System Con- The System Control module contains four different display panes that can be opened
trol panes all at once or in any combination:
• The Run Data pane.
• The Curves pane.
• The Flow Scheme pane.
• The Logbook pane.

The Run Data The Run Data pane displays the current values for the selected run parameters. The
pane values are updated at regular intervals, which are defined in the system strategy.
See the illustration below:

The Curves pane The Curves pane displays monitor signal values graphically. See the illustration
below:

03-0014-90 • p 32
 UNICORN concepts 2

The Flow Scheme The Flow Scheme is a graphical representation of the chromatography system.
pane During a run, the Flow Scheme displays open flow paths in color. Monitor signals
can be displayed numerically. See the illustration below:

The Logbook The Logbook pane displays all actions during a separation run, e.g. method start
pane and end, base instruction, method instructions and manual instructions such as
Pause or Hold. See the illustration below:

The Status bar The Status bar in the bottom of the System Control module displays the current
status of the separation run. See the illustration below:

The current system status is represented by the colored dot:

• A green dot represents a running system.
• A red dot represents a system in Pause state.
• A yellow dot represents a system in a Hold state.
• A white dot represents a system in an End state.

Toolbar icons in The table below describes the toolbar icons in the module:
the System Con-
trol
Icon Function
The Run icon opens the Run dialog box, which shows
all available methods. If a method is loaded, Run Setup
opens.

• p 33
 2 UNICORN concepts
2.2 The UNICORN user interface
2.2.3 The System Control module

Icon Function
The Hold icon suspends execution of the method,
while liquid is still pumped at the current flow rate
and eluent concentration.

The function of the Pause icon depends on the

strategy. The Pause icon suspends execution of the
method and stops all pumps so that the system comes
to a standstill.

The Continue icon resumes the execution of a paused

or held method.

The End icon terminates the method execution and

puts the system into an End state.

The Customise Panes icon opens the Customise Panes

dialog box, which is used to select the display panes
that are open.

The View Documentation icon opens the documenta-

tion pages. Run notes can be entered in the Notes page
and settings can be changed.

The View Properties icon opens the Properties dialog

box, which is used to control the data display in the
System Control panes.

The Connect System icon is used to connect a system.

The Disconnect System icon is used to disconnect the

system.

The Take Control of the System icon is used to leave

the view mode for the system and change into a con-
trol mode.

The Leave Control of the System icon is used to leave

the control mode for the system and change into a
view mode.

03-0014-90 • p 34
 UNICORN concepts 2

2.2.4 The Evaluation module

Introduction The Evaluation module provides extensive facilities to present and to evaluate curve
data.

The module win- Opened result files are displayed in the Evaluation module window. See the
dow illustration below:

Toolbar icons in The table below describes the toolbar icons in the module:
the Evaluation
module
Icon Function
The New icon opens an empty chromatogram.

The Open icon displays all available result files and result folders in
the Open Result dialog box.

The Open Curves to Compare icon opens the Open Curves to Compare
dialog box, which is used to select and open curves for comparison.

The Save icon saves the edited result file.

The Print icon opens the Print Chromatograms dialog box.

• p 35
 2 UNICORN concepts
2.2 The UNICORN user interface
2.2.4 The Evaluation module

Icon Function
The Report icon opens the Generate Report dialog box, which is used
to select a report format.

The View Documentation icon opens the Documentation dialog box,

which is used to view and edit the result documentation.

The Peak Integrate icon opens the Integrate dialog box, which is used
to select peaks to integrate in a modified peak table.

The Chromatogram Layout icon opens the Chromatogram Layout dialog

box, which is used to select and format curves and display items in
the chromatogram.

The Multifile Peak Compare icon opens the Multifile Peak Compare
Wizard, which is used to compare peak data from different result
files.

03-0014-90 • p 36
 UNICORN concepts 2

2.2.5 Search functions

Introduction This section describes the general search functions that can be used to locate for
example chromatograms, curves and text strings in UNICORN. These functions
can be used in several program modules, dialog boxes and wizards.

Search the Folder The search will take place in the displayed folder only. To select another folder,
list click the Browse button and open the desired folder.

Search the Result • The search will take place in all result files within the selected folder as denoted
list by the asterisk (*). To select specific result file(s), click the Browse button and
select the result file(s).
• You can use wildcard characters to search for chromatograms within result files
with a specific name profile.
- * represents any number of characters
- ? represents any single character

Wildcard character examples:

iex will search files named “iex”
iex* will search all files with names that begin with “iex”
*iex will search all files with names that end with “iex”
?iex will search only 4-character names that end with iex

Search the Chro- The asterisk (*) indicates that all chromatograms within a result file will be selected.
matogram list Click Browse to select one or several specific chromatograms.

Search the Curve The UV curves are identified by number and sometimes wavelength. For example,
name list UV1_280, UV2_280 and UV1_254 are all different curves. To search for all UV
curves, select *UV* in the Curve name text field.

Searches for A Sample ID can be used as a search criteria if it has been defined as a variable.
Sample ID The Sample ID can be entered in searches for result files both in the UNICORN Manager
and in the Evaluation module.

• p 37
 2 UNICORN concepts
2.2 The UNICORN user interface
2.2.5 Search functions

Find a text string The Find command is used to search for text strings:

Field Description
Find what Type the text string you want to find.

Match whole word only Select the check-box if you only want complete string
matches, not partial matches.

Match case Select the check-box if you only want matches which
correspond according to upper-case and lower-case
letters.

Search from top of docu- Select the check-box to start the search from the top
ment of the document, otherwise the search will start from
the cursor position.

Direction Choose whether to search upwards or downwards in

the document.

Commands
Use the commands below to find more occurrences of a text string after you have
found the first one:
• Press F3 to search for the next occurrence of the string or right-click and choose
Find next.
• Right-click and choose Find previous to search for a previous occurrence.

General informa- • The default setting is to search in all result files or chromatograms.
tion about
searches • User-entered search filters (to a maximum of 10) will be saved in the drop-down
menus for both Result and Chromatogram selections. More than one string can
be used as a search delimiter (insert “;” between strings), and search filters are
automatically saved and stored within user profiles.
• Click All to return to the default setting to search in all result files or
chromatograms.

03-0014-90 • p 38
 UNICORN concepts 2

2.2.6 Help functions and manuals

Introduction There are different ways to get help and instructions in the UNICORN application:
• From the Help menu in each module
• From the context-sensitive help in each dialog box
• By selecting the Online Manual from the Help menu
• By pressing the <F1> key
• By right-clicking an instruction in the Method Editor and selecting the What’s
This? menu item

The Help menu • From the Help menu in each module you can access the Help file.
• From the Help menu of the UNICORN Manager module you can also access the
installed manuals.

The illustration below shows the Help menu of the UNICORN Manager module:

The Help file The table below describes how to open and use the Help file:

Step Action
1 Choose Help:Index.
Result: The Help file is displayed

2 • Type a word you want help on in the text box in the left pane.
Result: The closest matches are displayed in the list.
• Select a match and click the Display button.
Result: The associated help text is displayed in the right pane.

3 • You can also click the Contents tab to view the contents of the
Help file divided into sections.
• Click the plus signs to expand the tree structure.
• Click a topic to read the associated help text.

• p 39
 2 UNICORN concepts
2.2 The UNICORN user interface
2.2.6 Help functions and manuals

Manuals When UNICORN was installed, the administrator selected which manuals to
install. Therefore the available manuals may be different on your system than in
the illustration below.
Note: Manuals can be added after the UNICORN installation. See the
Administration and Technical manual for more information.
How to open a manual
To open a manual
• choose Help:Manuals in the UNICORN Manager module.

Result: The Manuals dialog box is opened.

• Select the manual and click the OK button.

Note: Some manuals are only available in PDF format.

Context-sensitive In each dialog box there is a Help button. If you press that button, either of the
help following will be displayed:
• A message box with relevant information, for example the dialog box options.
• The Help file, with relevant information displayed in the right pane.

03-0014-90 • p 40
 UNICORN concepts 2

2.2.7 Snapshots

Introduction A Snapshot provides information about a method run at a certain point in time. It
contains information about the values of all the variables at the selected point.
Snapshot functionality is available in
• the Method Editor, where Snapshot instructions can be inserted in a method to
be recorded during the method run.
• the Evaluation module, where you can take Snapshots from a result file using
the Marker.
• the System Control module, where you can take Snapshots during a run using
the Marker.

How to view recor- The table below describes how to view Snapshots which have been recorded during
ded Snapshots a method run using the Snapshot text instruction.
Note: How to insert the Snapshot text instruction in a method is described in 5.3
How to use Text instructions on page 90.

Step Action
1 In the Evaluation module,
• choose View:Documentation

or
• click the View Documentation icon.

Result: the Documentation dialog box is displayed.

2 • Select the Result Information tab.

• Select the Snapshots sub-tab.

Result: The recorded Snapshot information for a chromatogram is

displayed in a list.

3 You can
• select other chromatograms in the Select chromatogram drop-
down box.
• select the Rows or Columns radio button to display each Snapshot
as a row or a column.
• select the Time or Volume radio button depending on which
quantity you want as a base.

• p 41
 2 UNICORN concepts
2.2 The UNICORN user interface
2.2.7 Snapshots

Step Action
4 To print the Snapshot information
• click the Print button
• select the Snapshot check box in the Print dialog box.
• click OK.

5 Click OK (or the Cancel button) to exit the Documentation dialog box.

How to take The table below describes how to take Snapshots in the Evaluation module:
Snapshots in the
Evaluation mod-
ule Step Action
1 • Open a result file in the Evaluation module.
• Right-click and select Marker in the menu.

Result: A vertical line indicating a certain point is displayed.

2 Click the marker line and drag it to the desired point where you
want to take a Snapshot.

3 Right-click and select Snapshot in the menu.

Result: The Snapshot is displayed in the Snap Shot dialog box.

4 • Click the Save to File button if you want to save the information
as an Excel file (.xls) or a tabbed text file (.txt).
• You can also copy the information to the clipboard:
- Click and drag the mouse in the table to select the information
you want to copy.
- Press CTRL+C.
The information can now be pasted in a text editor.
• Click the Print button if you want to print the information.
• Click the Close button.

03-0014-90 • p 42
 UNICORN concepts 2

Step Action
5 Repeat steps 2 to 4 if you want to view more Snapshots.

How to view The table below describes how to view Snapshots in the System Control module
Snapshots during during a method run:
a method run

Step Action
1 A method is running and the System Control is displayed:
• Right-click in the Curves pane and select Marker in the menu.

Result: A vertical line is displayed.

2 Click the marker line and drag it to the desired point where you
want to take a Snapshot.

3 Right-click in the Curves pane and select Snapshot in the menu.

Result: The Snapshot is displayed in the Snap Shot dialog box.

4 • Click the Save to File button if you want to save the information
as an Excel file (.xls) or a tabbed text file (.txt).
• You can also copy the information to the clipboard:
- Click and drag the mouse in the table to select the information
you want to copy.
- Press CTRL+C.
The information can now be pasted in a text editor.
• Click the Print button if you want to print the information.
• Click the Close button.

5 Repeat steps 2 to 4 if you want to view more Snapshots.

• p 43
 2 UNICORN concepts
2.3 Quick Start Guide

2.3 Quick Start Guide

Introduction This guide is intended for users who are fully familiar with the safety precautions
and operating instructions that are described in all manuals, i.e. experienced users
of previous versions of UNICORN. The instructions assume that all installations
were made according to the instructions, that the model system is used and is
connected.

Quick Start in- The table below describes the easiest way to create a method, run the system and
structions generate a printed chromatogram. The procedure is based on an Instant run.

Step Action
1 Click the Instant run icon in the UNICORN Manager module.

Result: The Instant run dialog box opens.

2 • Select Wizard.
• Select a system (if necessary).
• Click the Run button.

Result: The Method Wizard opens in the System Control module.

3 Go through all selections on the Method Wizard pages. Click the Next
button to proceed from page to page.

4 Click the Run button on the last page.

Result: The start protocol opens.

5 Verify the method on the Variables page and change values as re-
quired. Click the Next button to proceed through several pages.

6 Select Print_Chromatogram in the Evaluation procedures page.

Result: A printout will automatically be generated after the run.

7 Click the Start button on the last page.

Result: The run starts.

03-0014-90 • p 44
 General system operations 3

3 General system operations

Introduction This chapter describes how to start the program, assign user properties and set up
the system.
Refer to the Administration and Technical Manual for installation and network
configuration instructions.

In this chapter This chapter contains these sections.

Topic See
Log on and log off routines 3.1

How to create a new user 3.2

How to assign user properties 3.3

How to change your passwords and user attributes 3.4

How to connect to the chromatography system 3.5

How to back up and restore system data 3.6

How to set up a printer 3.7

• p 45
 3 General system operations
3.1 Log on routines and log off routines

3.1 Log on routines and log off routines

Introduction This section describes how to start and quit the UNICORN program, and how to
log on and log off.

Username and Normally the system administrator defines the users and creates your first password.
password The program can also be set up so you can log on without a password.
Note: The first time after UNICORN has been installed, you may need to log on
as a default user and create a user profile. This process is described in 3.2 How to
create a new user on page 50.

How to start the Note: if UNICORN is already started by a previous user, proceed to How to log
program on.
There are two ways to start the program:

If you start with... Then...

a UNICORN icon on double-click the icon
your desktop

the Windows Start locate the program under Programs:Unicorn and click
menu in Windows the UNICORN logo
2000

the Windows Start locate the program under All programs:Unicorn and
menu in Windows XP click the UNICORN logo

How to log on The table below describes how to log on to UNICORN.

Step Action
1 • Select Tools:Logon in the UNICORN Manager module

or
• Click the Logon/Logoff icon in the UNICORN Manager module

Result: the Logon dialog box is displayed.

Note: You do not have to perform this step if you start up
UNICORN. When you start UNICORN the Logon dialog box is
automatically displayed.

03-0014-90 • p 46
 General system operations 3

Step Action
2 Select your username from the list.

3 Type your password (optional).

4 Click OK.

The four program The program has four modules. When you start the program and log on you work
modules in the UNICORN Manager module. UNICORN also automatically opens the Method
Editor, the System Control and the Evaluation modules. These modules are minimized
until you activate them. Up to four System Control module windows may open if
UNICORN was set up to control more than one system at the installation.
Note: If the access rights are limited to only some modules, the other modules will
not open.

Log off after you Always log off when you leave the computer to prevent others from accidentally
are finished changing or deleting your files, or disturbing your UNICORN runs. There are two
ways to log off in the UNICORN Manager:
• Select Tools:Logoff

or
• Click the Logon/Logoff icon.

Note: In case your access to the UNICORN Manager is restricted you will still be able
to log off.

Processes can run The process will continue even if you log off while a separation run is in progress.
after log off You can leave the process locked and set a password to protect it from interference.
The table below describes how to log off and set a password for a running process.

Step Action
1 • Select Tools:Logoff in the UNICORN Manager module.

or
• Click the Logoff icon.

Result: A confirmation box opens.

2 Click Yes to confirm that you want to log off.

Result: The Leave Control of system dialog box opens.

3 Click the Locked radio button.

• p 47
 3 General system operations
3.1 Log on routines and log off routines

Step Action
4 Type a password in the Password text box.

5 Click OK.

Unlocked Log off It is not recommended that you log off and leave a running system unlocked. This
means that the run is in progress without a user that is responsible for the process.

Automated work- The system administrator may set an automatic workstation lock or log off after
station lock or a specified time for a user. If there are no keyboard entries or mouse movements
logout
within the time limit, the workstation will be locked or logged off.
Note: A locked workstation can be activated again only by the previous user if the
regular log in password is entered. If another user wants to log on and use the
workstation the previous user can be logged off without entering the correct
password. The previous user’s files will be closed and the new user will only have
access to his own files. Automated logout will not happen while a MethodQueue or
a Scouting scheme is operating.

How to log on When you log on again after leaving the system locked with a process running or
and unlock the after an automated workstation lock, you will be asked to unlock the system.
system

Step Action
1 Log on to the system.
Result: The System Unlock Confirmation dialog box opens.

2 Type your login password or the password that the system was
locked with in the Password text box.

3 Click OK

Note: You can connect in view mode only without providing the password.

Systems locked by You can unlock a system that has been locked by another user if you have the
other users correct password.
You may still be able to unlock a system even if you do not have the password.
Any user with Unlock locked systems authorization can override another user’s
lock by entering his or her own logon password. However, it is recommended that
this authorization is limited to only a few users.

03-0014-90 • p 48
 General system operations 3

How to quit UNICORN will still be open after you have logged off. To close the program you
UNICORN must log in again and quit UNICORN (you cannot quit the program if you are
not logged in). The table below describes how to do this.

Step Action
1 • Select the File:Quit Program menu command in the UNICORN Man-
ager module.

or
• Click the close icon in the top right-hand corner of the program
window.

Result: A confirmation box opens.

2 Click Yes to confirm that you want to quit.

3 A Warning opens if you have any unsaved data in the Method Editor
or Evaluation module.
• Click Yes to continue to close the program. Your unsaved data
will be lost when the program is closed.
• Click No to return to the program and save your data.

4 The Leave Control of system dialog box opens. Select the locked or
unlocked option as in the logoff procedure.
Note: This step only happens when a system is connected.

5 Click OK.

Note: Do not shut down Windows 2000/XP or turn off the computer if you quit
UNICORN with a separation run in progress. If you are performing a Scouting
run or a MethodQueue run you cannot quit the program at all.
In case your access to the UNICORN Manager is restricted you will still be able to
quit the program.

• p 49
 3 General system operations
3.2 How to create a new user

3.2 How to create a new user

Introduction This section describes how to create a new user and assign a home folder for the
user’s methods and results.

Default user A default user is created when the system is installed. The default user has
unrestricted access to all UNICORN functions. You log on with this profile when
you access a newly installed system for the first time.
The table below describes how to log on as the default user.

Step Action
1 Select user default from the user drop-list.

2 Type password default if necessary.

Note: The default user is the only user that is allowed to use the user
name as password.

3 Click OK or press the Enter key.

Note: We recommend that the default user is deleted when regular user profiles
are created.

03-0014-90 • p 50
 General system operations 3

How to open User All user administration is performed in the User Setup dialog box in the Main Menu
Setup module. It is accessible only to authorized users (and the default user).
User Setup is found on the Administration menu.
• Choose Administration:User Setup.

The User Setup The illustration below shows the User Setup dialog box.
dialog box

How to create a The table below describes how to create a new user.
new user
Step Action
1 Click the New button in the User Setup dialog box.
Result: The Create New User dialog box opens.

• p 51
 3 General system operations
3.2 How to create a new user

Step Action
2 Enter a user name in the User name text box.

3 Enter the full name of the user in the Full name text box.

4 Enter the position of the user in the Position text box.

5 Select or create a Home folder:

• Select a Drive and a folder from the Name drop-list and proceed
to step 9.

or
• If you need to create a new home folder, proceed with step 6.

6 Click New.
Result: the Create New Folder dialog box opens.

7 Select a Drive and type a folder name.

8 Click OK to create the folder and return to the Create New User dialog
box.

9 Click OK.
Result: The new user is created and added to the User Setup list.

10 • Click Close.

or
• Click the New button and repeat steps 1 - 8 to create more users.

Home folders Each user must be assigned to a home folder. The Default folder can be used if you
do not want to assign an individual home folder.
Note: If you create a home folder on the C: (local) drive it will not be accessible
from other computers. If you select a network, make sure that is addressed by the
same drive letter from all computers in the network.

03-0014-90 • p 52
 General system operations 3

3.3 How to assign user properties

Introduction A user is assigned properties that define password rules, and the folders and
chromatography systems that the user can access. This section describes how to
assign properties.

How to open User The user properties are defined in the User Setup dialog box in the UNICORN Manager
properties module. The table below describes how to open User Setup.

Step Action
1 Select Administration:User Setup.

2 Select a user in the Users list.

3 Click the Edit button.

Result: The User properties dialog box opens.

The User properties dialog box is used to edit the user definition and assign
properties for passwords, folder and system access, and available manual
instructions.

How to edit the The table below describes how to edit the user definition in the User properties
user definition dialog box.

Step Action
1 Select the User item.

2 Select an access group from the Group drop-down box.

Note: A pre-defined access group is assigned a certain level of access
to UNICORN.

3 Select a folder from the Home folder drop-down box.

4 Click the check boxes to select Administrator Attributes.

5 Click OK to finalize or select another definition to edit.

How to edit the The table below describes how to edit the attributes in the User Attributes window
user attributes pane.

Step Action
1 Select the Attributes item.

• p 53
 3 General system operations
3.3 How to assign user properties

Step Action
2 Select applicable attribute items in the User Attributes pane:
• Use large toolbar icons
• Show unused variables
• Show variable details
• Default overwrite of baselines and peak tables
• Prompt for column before manual runs

3 Type which curve to display in the Quick view dialog box.

4 • Select a size definition and type a value for the Fraction mark
height.
• Select a size definition and type a value for the Injection mark
height.
• Select a size definition and type a value for the Logbook mark
height.

5 Click OK to finalize or select another definition to edit.

The Advanced The Advanced window pane is used to define password policies for the user.
dialog page Normally this is only used by the system administrator.

03-0014-90 • p 54
 General system operations 3

Note: This dialog page is only available if a required password was selected when
the software was installed.

How to define ac- The Access dialog page is used to define the folders and systems that the user has
cess to folders and access to. Click the check box for each selected folder and system.
systems
Up to 20 folders can be set up to be shared. The user has access to all files and
sub-folders in the selected folders. Only selected folders will be visible in the methods
or results panels of the UNICORN Manager module.
Note: All users should have access to the Failed folder on each local station in a
network installation. This will ensure that users can access results that were saved
in the Failed folder in case of a network communication error.

How to define The Instructions dialog page is used to define the manual instructions and system
available manual sounds that are available to the user as well as which monitors the user is allowed
instructions
to calibrate. Click the check box for each selected instruction, sound or monitor.

Access groups The level of access to UNICORN functions for each user is determined by the
Access group that the user is assigned to. The access authorizations can be edited
for each group, normally by the systems administrator. Refer to the Technical and
Administration Manual if you need to edit an Access group.
Note: User access can be limited to only some UNICORN modules. If that is the
case the unavailable modules will not be displayed. E.g. if the UNICORN Manager
is unavailable you will only have access to a dialog box with the basic functions
to change limited user attributes, passwords and to log out and quit the program.

• p 55
 3 General system operations
3.4 How to change your passwords and user attributes

3.4 How to change your passwords and user attributes

Introduction Every user can change his or her passwords and some user attributes even if user
administration is handled exclusively by the system administrator. The changes
are made in the UNICORN Manager.

How to change The table below describes how to change your logon and signature passwords.
passwords
Step Action
1 Select Administration:Change Password.
Result: The Change Password dialog box opens.

2 Type your old logon password in the Old text box.

Note: Your passwords will only be shown as asterisks.

3 Type a new password in the New text box.

4 Repeat the new password exactly in the Confirm text box.

5 Repeat steps 2 to 4 in the Signature password section if necessary.

6 Click OK.

About passwords The list below is a summary of facts and advice about UNICORN passwords:
• The system can be set up to operate without required passwords.
• The minimum number of password characters is set up at installation.
• Passwords can be any combination of letters and numbers.
• Passwords are case sensitive.
• Avoid using obvious passwords, e.g. your username, your telephone number,
etc.
• The settings in the User properties determine the expiration for a password.
Change passwords regularly even if your user profile is set up without password
expiration.

03-0014-90 • p 56
 General system operations 3

How to change The table below describes how to change your user attributes.
user attributes
Step Action
1 Select Administration:Change User Attributes.
Result: The Change user attributes dialog box opens.

2 Dialog check box options

The dialog check box options are described below:
• Use large toolbar icons
Display large toolbar icons in all modules.
• Show unused variables
Show variables that are not used in the method on the Variable
page of the Start Protocol.
• Show variable details
Show detailed method variables on the Variable page of the Start
Protocol.
• Default overwrite of baselines and peak tables
When new baselines and peak tables are created, the old ones are
overwritten.

3 Mark heights
Select a size definition and type the height for the following marks:
• Fraction mark
• Injection mark
• Logbook mark

4 Click OK.

• p 57
 3 General system operations
3.5 How to connect to the chromatography system

3.5 How to connect to the chromatography system

Introduction A computer can have up to four chromatography systems connected at a time. This
section describes how to connect to the systems, and different connection modes.

How to establish The table below describes how to connect a chromatography system that is locally
a connection connected to your computer.

Step Action
1 Open a System Control module.
Note: Each UNICORN installation may have up to four System
Control modules. The number of modules are selected when the
software is installed.

2 • Select the System:Connect menu command.

or
• Click the Connect to system toolbar icon.

Result: The System Connect dialog box opens.

3 Select the system you want to connect.

4 Click OK.

Remote connec- Each computer workstation may have up to four chromatography systems connected
tions locally. In a network installation you may connect a system that is physically
connected to another computer, the local station. Your system is then a remote
station.
The local station that is connected to the chromatography system must be logged
on to the network and the UNICORN drivers must be running. However, the
connection will work even if the UNICORN program is not running on the local
station.

03-0014-90 • p 58
 General system operations 3

Network log on Ensure that your workstation is logged on to the network before you start a
chromatography system that is directly connected to the station. You can operate
a local system without logging on to the network, but there are several disadvantages
to this:
• Files stored on network drives are not accessible.
• Changes made to global files, e.g. user settings files, will apply only locally and
will be lost the next time you log on to the network.
• Result files that are directed to a network drive will be stored in the Failed folder
on the local station.

Connection modes Several workstations can connect to a single chromatography system at the same
time but only one workstation can be in control mode. The other connections are
in view mode and the connected workstations can only monitor the system activity,
but not issue any commands.
The system status is indicated on the status bar at the bottom of the System Control
window. The table below describes the different connection modes, the
corresponding status texts and some of the various actions you can take to change
the connection mode.

Connection mode Status Text Possible action to

change connection mode
Not connected Connect to a system.

Control mode. Disconnect from or

leave control of the sys-
tem.
(The system is controlled
by you.)

View mode No connection possible.

(The system is controlled
by another user.)

View mode Click the Connect to sys-

tem icon and supply a
password.
(The system is locked by
another user.)

View mode Connect to the system.

(The system has been
left unlocked.)

• p 59
 3 General system operations
3.5 How to connect to the chromatography system

How to leave con- The table below describes how to leave control of a system so that it is available
trol of a system to be controlled by other users.

Step Action
1 • Select System:Leave Control.

or
• Click the Leave control of system icon.

Result: The Leave Control of system dialog box opens.

2 Click the radio buttons to select to leave the system unlocked or

locked.

3 Enter a password (if the system is to be locked).

4 Click OK.

How to discon- The table below describes how to disconnect from a system.
nect a system
Step Action
1 • Select System:Disconnect.

or
• Click the Disconnect from system icon.

2 Result:
If the system is in view mode
• the system is disconnected.

If the system is in control mode

• the Leave Control of System dialog box opens.

3 Select to leave the system locked or unlocked.

4 Click OK.
Result: The system is disconnected.

03-0014-90 • p 60
 General system operations 3

How to discon- When you log off or quit from UNICORN you automatically disconnect all
nect when quitting connected systems. A Leave Control of System dialog box will be opened for each
system that was connected.
Note: If you disconnect from a system in control mode and re-connect to it, you
may be connected in view mode. Another user may have taken control in the
meantime.

How to view or You can view and print a total summary of a selected system from the System Table
print a system Summary dialog box.
summary
The table below describes how to view and print an information summary of a
selected systemthe systems:

Step Action
1 Choose Administration:System Setup in the UNICORN Manager.
Result: The System Setup dialog box is displayed:

• p 61
 3 General system operations
3.5 How to connect to the chromatography system

Step Action
2 • Select the system you want a summary of.
• Click the Summary button.

Result: The System Table Summary dialog box is displayed:

3 • Click the Print button to print the information.

• Click the Close button to exit the dialog box.

03-0014-90 • p 62
 General system operations 3

3.6 How to back up and restore system data

Introduction You can create a backup file with system information and store it on a diskette or
another drive. The backup file will contain information about
• Global Files
• Personal Files
• System Files

Afterwards you can use the backup file to restore the system definitions in case
they are corrupted.

How to create a The table below describes how to create a backup file and store it for example on
backup file a rescue diskette:

Step Action
1 Insert a diskette into the computer if you want to store the backup
file on a diskette.

2 Choose Administration:Create/Restore Backup in the UNICORN Manager

to display the Create/Restore Backup dialog box:

• p 63
 3 General system operations
3.6 How to back up and restore system data

Step Action
3 • In the Action field, make sure that the Create option is selected.
• Click the Browse button to select where to store the backup file.
.Note: Select A:\ to store the file on the diskette.
• In the Items field, select which information to include on the
backup file.
• Click the Create button to create the backup file and store it in
the selected location.

Note: You can click the Information button to see which information
files will be included in the backup file.

How to restore The table below describes how to restore the system data from a backup file, located
the system data for example on a rescue diskette:

Step Action
1 If the backup file is located on a diskette, insert the diskette into the
computer.

2 Choose Administration:Create/Restore Backup in the UNICORN Manager

to display the Create/Restore Backup dialog box:

03-0014-90 • p 64
 General system operations 3

Step Action
3 • In the Action field, select the Restore option.
• Click the Browse button to select the folder where the backup file
is located.Note: Select A:\ if the file is located on the diskette.
• In the Items field, select which information to include from the
backup file.
• Click the Restore button to restore the system definitions.

Note: You can click the Information button to see which information
files are included in the backup file.

• p 65
 3 General system operations
3.7 How to set up a printer

3.7 How to set up a printer

Introduction UNICORN uses the default printer and printer settings that are installed on your
computer. You can change your printer by changing the default Windows settings,
but you can also set up a printer in UNICORN for the current working session.

How to set up a The table below describes how to set up a printer in UNICORN.
printer
Step Action
1 Select the File:Printer Setup menu command in the UNICORN Manager
module.
Result: The Print Setup dialog box opens.

2 Select a printer from the Name drop-down box.

3 Change all printer properties as necessary.

4 Click OK.

Note: To save created reports electronically you can select to print the files in
PDF-format. To be able to do this you must have a full version of Adobe™
Acrobat™ installed and select PDF Writer or Distiller™ in the Printer Setup.

03-0014-90 • p 66
 Files and folders in UNICORN 4

4 Files and folders in UNICORN

Introduction All UNICORN data is organized in files and folders. Files and folders are handled
like in any other Windows application, with some exceptions. This chapter describes
how to work with UNICORN files and folders, with the focus on the topics that
are specific for UNICORN.

In this chapter This chapter contains these sections.

Topic See
How to create folders 4.1

How to open and preview files 4.2

How to arrange and locate your files 4.3

How to copy, delete, rename and back-up files and folders 4.4

• p 67
 4 Files and folders in UNICORN
4.1 How to create folders

4.1 How to create folders

Introduction This section describes how folders are organized in UNICORN and how to create
a new user-specific folder for the user’s methods and results.

UNICORN The files and folders are displayed in the two UNICORN Manager module windows.
folders
• All method files and corresponding folders are listed in the Methods window.
• The result files and folders are listed in the Results window.
• You can only see folders that you have access to.
• You can only see method files that are written for systems that you have access
to.

How to create a The table below describes how to create a user-specific folder.
user-specific folder
Step Action
1 Select the window you want to create the folder in: Methods or Res-
ults.
(Result: The window title bar is highlighted.)

2 • Select File:New:Folder.

or
• Right-click and select the New Folder shortcut.

Result: The Create New Folder dialog box opens.

3 Type a name for the new folder.

4 Click OK.

03-0014-90 • p 68
 Files and folders in UNICORN 4

4.2 How to open and preview files

Introduction This section describes how to open your saved method files and result files. You
can also preview your result files to identify the correct file before you open it.

How to open a You open a method file in the UNICORN Manager module. Click the file in the
method file Methods window to select it and
• choose File:Open.

or
• right-click the file and choose Open from the short-cut menu.

or
• double-click the file.

Result: The file is opened for editing in the Method Editor module.
Note: A method file cannot be opened on two workstations simultaneously.

How to open a You can open a result file in the UNICORN Manager module. Click the file in the
result file in Results window to select it and
UNICORN Man-
ager • choose File:Open.

or
• right-click the file and choose Open from the short-cut menu.

or
• double-click the file.

Result: The file is opened for editing in the Evaluation module.

How to open a The table below describes how to open a result file from the File Navigator in the
result file in the Evaluation module.
Evaluation mod-
ule
Step Action
1 • Click the Files tab.

• p 69
 4 Files and folders in UNICORN
4.2 How to open and preview files

Step Action
2 • Locate and double-click the result file

Result: The result file opens.

Note: The File Navigator is opens by default in the Evaluation module. If it has been
closed, select View:File Navigator in the Evaluation module.

Quick View Quick View is a preview function for result files to make it easier to select the
correct result file.
You can preview the first curve in the first chromatogram. You can also select to
view another curve as default by selecting another curve number in your User
Attributes settings, see 3.4 How to change user attributes on page 57.
Several files can be opened for comparison.

How to use Quick The table below describes how to preview result files in Quick View.
View
Step Action
1 Select one or more result files in the Result window of the UNICORN
Manager.

03-0014-90 • p 70
 Files and folders in UNICORN 4

Step Action
2 • Choose File:Quick View.

or
• Right-click and choose Quick View from the short-cut menu.

Result: The Quick View dialog box opens.

3 • Click the Next and Previous buttons to move between the result
files (if more than one is selected).
• Click the Open button when the right file is displayed.

Result: The result file that is displayed in the dialog box opens in
the Evaluation module.

• p 71
 4 Files and folders in UNICORN
4.3 How to arrange and locate your files

4.3 How to arrange and locate your files

Introduction This section describes how to arrange the way the files are displayed in your
UNICORN workspace and how to locate files through a search.

Different view You can choose how the files and folders are displayed in the UNICORN Manager
modes windows. The options are the standard Windows alternatives:
• Details
• List
• Large icons
• Small icons.

How to change If you want to change the view you either:

the view mode
• Select View and the option that you want,

or
• Right-click and select View and the option that you want from the shortcut
menu.

Sort order in de- The files can be sorted in a different order when a window is displayed in detailed
tailed view view. The table below shows the options.

Sorted by: Order

Name Alphabetical order or reverse alphabet-
ical order.

System Alphabetical order or reverse alphabet-

ical order (Method window only).

Size Smallest or largest files first.

Type Alphabetical order of file extension

type.

Modified Most recently modified files first.

Created Most recent creation dates first.

How to change Select one of the methods below to change the sorting order:
the sorting order
• Select View:Sort and the option that you want,

or

03-0014-90 • p 72
 Files and folders in UNICORN 4

• Right-click and select Sort and the option that you want from the short-cut
menu.

or
• Click the column header for the option that you want to sort by (a second click
on the same header will reverse the order).

Note: Only the currently active window is affected.

How to filter The files in the Method window can be filtered to show only methods for selected
Method files systems. You can also limit the displayed files by using standard Windows wildcard
characters. The title bar of the Method window indicates if a filter has been activated.
The table below describes how to activate a filter.

Step Action
1 • Select View:Filter.

or
• Right-click and select Filter from the shortcut menu.

Result: The Filter dialog box opens.

2 Click the check-boxes for the systems for which you want to show
files.

3 Enter a file name specification (if necessary).

4 Click OK.

• p 73
 4 Files and folders in UNICORN
4.3 How to arrange and locate your files

How to find files The table below describes how to perform a search for files.

Step Action
1 Click either the Methods or Results window and:
• Select the File:Find menu command.

or
• Right-click and select Find from the shortcut menu.

Result: The Find files dialog box opens.

2 Add search criteria to the dialog box, for example:

• Type a name in the Name field.
• Select a file type from the Type drop-down box.
• Select if the search should include subfolders.
• Select date limits in the Date drop-down boxes.
• Type text strings to match Question or Answer texts.
• Type a variable name and, if desired, a value.
• Type a Batch ID.

Note: You can search for a sample ID provided the sample ID is

defined as a variable.

3 Click Find.
Result: The search results are listed in the Found folders and files
field. The search is limites to either methods or results and to the
folder (including its subfolders) that is currently displayed.

4 Double-click a file in this list.

Result: The dialog box is closed and the selected file is highlighted
in the UNICORN Manager window.
Note: If you click Close you will return to the UNICORN Manager
window with no file highlighted regardless if you have selected one
in the dialog box or not.

03-0014-90 • p 74
 Files and folders in UNICORN 4

4.4 How to copy, delete, rename and backup files and

folders

Introduction UNICORN has some file and folder handling functions that are slightly different
from the general Windows functions. This section focuses on the differences.
Note: You need explicit authorization in your user profile to copy, move and delete
files.

How to copy or There are some restrictions to how you can copy or move files and folders:
move files and
folders • Files and folders can only be copied or moved to folders that are specific to your
user name.
• You can also copy files to and from the folders that you have access to on the
network.
• Method files or folders cannot be copied to the Results window.
• Result files and folders cannot be copied to the Methods window.

If you copy a folder you will also at the same time copy all files and folders that it
contains. The table below describes how to copy files and folders.
Note: Follow the same steps but select Move to move files and folders.

Step Action
1 Select one or more files and folders in either the Methods or Results
window of the UNICORN Manager.

2 • Select File:Copy.

or
• Right-click and select Copy from the short-cut menu.

Result: The Copy dialog box is opened.

3 Select a target folder or floppy disk drive.

4 Click OK.

The function Use the function Copy to External when you need to copy files and folders outside
Copy to External of your own user folders. Copy to External should be used specifically when you
need:
• to copy a method to another system (the method can then be connected to the
appropriate system),
• to copy to a floppy disk drive. (The files are automatically compressed into a
zip-file. The file will also automatically be spanned across several disks if
necessary.)

• p 75
 4 Files and folders in UNICORN
4.4 How to copy, delete, rename and backup files and folders

How to Copy to The table below describes how to use the function Copy to External.
External
Step Action
1 Select the file you want to copy.

2 • Select File:Copy to External.

or
• Right-click and select Copy to External from the shortcut menu.

Result: the Copy to External dialog box opens.

3 Select the destination drive and folder.

4 Click the Save button.

The function The function Copy from External can be used to import files and folders:
Copy from Extern-
al • If the files were saved using the function Copy to External they will automatically
be decompressed.
• Copied method files must be connected to the same type of system they originally
were created for. This is part of the Copy from External procedure.
• Method files that have been copied in and connected are displayed in the
designated folder in the Methods window.

How to use Copy The table below describes how to use the function Copy from External.
from External
Step Action
1 Select a destination folder in the Methods or the Results window.

2 • Select File:Copy from External.

or
• Right-click and select Copy from External.

Note: Do not select a file icon.

Result: The Copy from External dialog box opens.

3 Select the files you want to copy.

4 Click Save.
Result:
• Result files are copied into the designated folder in the Results
window.
• If method files were selected, the Method-System Connection dialog
box opens.

03-0014-90 • p 76
 Files and folders in UNICORN 4

How to connect a The table below describes how to connect a method to a system.
method to a sys-
tem
Step Action
1 Select a method and double-click a system.

Result: The method is connected and the system name is added after
the method name in the Method files list.

2 Repeat step 1 until all methods are connected to a system.

3 Click OK.

How to rename The table below describes how to rename files and folders in the Methods or Results
files and folders windows in the UNICORN Manager module.

Step Action
1 Select the item that you want to rename.

2 • Select File:Rename.

or
• Right-click and select Rename from the shortcut menu.

Result: The Rename dialog box opens.

3 Type a new name.

4 Click OK.

How to delete files The table below describes how to delete files and folders in the Methods or Results
and folders windows in the UNICORN Manager module.
Note: Home folders cannot be deleted this way.

Step Action
1 Select the item that you want to delete.

• p 77
 4 Files and folders in UNICORN
4.4 How to copy, delete, rename and backup files and folders

Step Action
2 • Select File:Delete.

or
• Right-click and select Delete from the shortcut menu.

or
• Press the Delete key.

3 Confirm the delete action in the confirmation dialog box

Backup security Backup copies should be taken regularly to avoid data loss in the event of hard
disk failure or accidental deletion. You can use the function Copy to External to
save your files on the network server.
Note: Amersham Biosciences cannot accept responsibility for the replacement of
method programs that were lost as a result of computer failure or other incidents.

03-0014-90 • p 78
 How to create a method 5

5 How to create a method

Introduction Chromatography runs are programmed as Methods in UNICORN. Before you can
proceed with a chromatography run you need either to use an existing method or
create a new method. This chapter describes how to create new methods. It also
contains instructions for signing a method.

In this chapter This chapter contains these sections:

Topic See
How to use the Method Wizard 5.1

How to use the Method templates 5.2

How to use Text instructions 5.3

How to sign the method 5.4

• p 79
 5 How to create a method
5.1 How to use the Method Wizard

5.1 How to use the Method Wizard

Introduction This section describes how to use a Method Wizard to create a new method. For
most purposes customized methods can be created simply by setting appropriate
values for the method variables.
Note: Each method is written for a specific strategy. The function of the method
cannot be guaranteed on systems having other strategies.

Are wizards al- Method Wizards are available for some ÄKTAdesign systems delivered with standard
ways available? strategies. Method Wizards are not available for process systems.

03-0014-90 • p 80
 How to create a method 5

How to create a The table below describes how to create a method with the Method Wizard.
new method
Step Action
1 Click the Method Wizard icon on the Method Editor module or choose
File:Method Wizard.

Result: The Method Wizard dialog box appears.

Note: If several systems are available you must first select which
system you want to use.

2 Select the appropriate parameter values and click the Next button.
Note: Click Set Default on the first wizard page to restore all settings
to the default values.

3 In each new dialog box, select the appropriate parameter values and
click the Next button to continue.
Note: Select a column even if you want to perform a test run without
a column. Use a small column. Replace the column with a piece of
tubing when you run the method.

• p 81
 5 How to create a method
5.1 How to use the Method Wizard

Step Action
4 Click the Finish button in the last dialog box.
Result: The Run Setup opens.

The Run Setup The Run Setup consists of a number of tabs. Click on the appropriate tab at the
top to select it.

The Variables tab The method is represented by a number of blocks on the Variables tab. The blocks
are typical steps in a chromatographic run.
Each block contains a number of Method Variables with suitable default values that
can be changed to suit your application. Only the most commonly used variables
are initially shown on the page. Click the Show details check box to display all
variables in the method.

Default values for When you select a column, default values will be set for several parameters including
columns the following:
• the correct column volume
• the recommended flow rate
• the correct pressure limit.

Note: If you exceed the recommended values for the selected column you will
receive a warning when you save your method.

03-0014-90 • p 82
 How to create a method 5

The Gradient tab The Gradient tab shows the method graphically:

The length of each block is marked at the bottom of the graph.

• Click the X-axis to view the method in time, volume or column volumes.
• Drag the Y-axis to display a marker in the gradient. The Base value, Gradient
value and Block name at the current marker position will be displayed in the
upper part of the graph.

How to save the A new method created from a Wizard is untitled, and must be saved under a method
new method name before it can be used. The table below describes how to save a new method.

Step Action
1 Click the Save Method toolbar or choose File:Save.

2 • If required, save the method in a folder other than the default

home folder.
• Enter a Method name for the method. The total path can be up
to 256 characters long. The method name must be unique for the
chosen system within the folder.

3 • If you have more than one system connected to the computer,

choose the System for which the method is intended. The method
can be run on any system that uses the same strategy. Remember
that different systems may have different configurations and
control capabilities.
• Choose the Technique for which the method was written.

• p 83
 5 How to create a method
5.1 How to use the Method Wizard

Step Action
4 Click OK.
Result: The method is saved, but remains open in the Method Editor,
so that you can continue editing if you wish.

Note: You might want to sign your method. If you do so, you can choose to lock
the method so that nobody will be able to change the method. See 5.4 How to sign
the method on page 91 for further instructions.

03-0014-90 • p 84
 How to create a method 5

5.2 How to use the Method templates

Introduction This section describes how to create methods based on an existing template.
Note: A custom system, for example a process system, requires that the users create
their own templates by saving methods as templates. Each method is written for
a specific strategy. The function of the method cannot be guaranteed on systems
having other strategies.

How to create a The table below describes how to create a method from the UNICORN Manager
new method module.
Note: The New Method dialog box is also accessible from the Method Editor module
using the same commands.

Step Action
1 • Choose the File:New:Method menu command

or
• click the New Method icon.

or
• right-click in the Methods window and select New:Method from
the shortcut menu.

Result: The New Method dialog box opens in the Method Editor
module.

2 • Select the system for which you want to create the method in the
For system drop-down list.
• Select Template in the Use field.
• Select a chromatographic technique from the Technique drop-
down list.
• Select a method template from the Template list.
• Select a column from the For column list and click OK.

Result: The method template will be opened as an untitled method

in the Run Setup in the Method Editor.
Note: If Any is selected in the For column list, you can use any column
but must enter the column volume in the method on the Variables
tab. It is recommended that a specific column is selected.

• p 85
 5 How to create a method
5.2 How to use the Method templates

Note: Only columns for the selected technique are displayed. If Any is selected as
technique, all columns are displayed. Right-click in the textbox to open a list of
the column categories to limit the number of displayed columns. If you type the
beginning of a column name in the textbox UNICORN will automatically complete
the column name.
If you do not find your specific column it can be added to the list. The column
value, recommended flow rate, pressure limit and averaging time for the selected
column will be automatically copied into the method, thus reducing the need to
edit the method.

Method notes Click the Notes and then the Method Notes tabs in the Run Setup. The notes describe
important information about the template and how the system should be connected
so that the method will work correctly.
Note: If your system does not correspond to the description on the Method Notes
tab, either:
• rearrange the valves and tubing connections in accordance with the method
notes description

or
• edit the method instructions in accordance with your system setup.

How to save the A new method created from a method template is untitled, and must be saved
new method under a method name before it can be used.
The table below describes how to save a new method.

Step Action
1 Click the Save Method toolbar icon or choose File:Save.

2 • If required, save the method in a folder other than the default

home folder.
• Enter a Method name for the method. The total path can be up
to 256 characters long. The method name must be unique for the
chosen system within the folder.

3 • If you have more than one system connected to the computer,

choose the System for which the method is intended. The method
can be run on any system that uses the same strategy. Remember
that different systems may have different configurations and
control capabilities.
• Choose the Technique for which the method was written.
• Click OK.

Result: The method is saved, but remains open in the Method Editor,
so that you can continue editing if you wish.

03-0014-90 • p 86
 How to create a method 5

Note: You might want to sign your method. If you do so, you can choose to lock
the method so that nobody else will be able to change the method. See 5.4 How
to sign the method on page 91 for further instructions.

• p 87
 5 How to create a method
5.3 How to use Text instructions

5.3 How to use Text instructions

Introduction You can use the Text Instructions editor in the Method Editor to build your method
step by step. You can also use the editor to modify instructions in methods created
by wizards or based on templates.
Advanced editing facilities are available when you work directly in the Text
Instructions editor. This section is a very brief description of this process. See 6
How to edit methods on page 92 for detailed instructions.
Note: Each method is written for a specific strategy. The function of the method
cannot be guaranteed on systems having other strategies.

When do I use Use Text Instructions when you want:

Text Instructions?
• to change selected instructions in the method, for example the outlet valve
position
• to add blocks or instructions, for example Watch instructions
• to change method instructions to adapt to non-standard system configurations
• to create new methods for applications not covered by the supplied templates
or wizards.

How to edit Text Open the Text Instructions editor by following the steps in the table below.
Instructions
Step Action
1 Select the Method Editor module and click the Text Instructions icon.

2 • Click the Customise Panes icon and select Text and Instruction Box.

• Click OK.

3 Select instructions in the Instruction box in the lower part of the

Method Editor, and use the Insert, Change, Replace or Delete buttons.
All text entries are shown in the Text pane. Applicable variables can
be edited for each selection.
The illustration below shows the Instruction box:

03-0014-90 • p 88
 How to create a method 5

Instructions can Individual text instructions can be grouped in blocks of instructions (marked by
be organized in blue square symbols) for a specific functional use, e.g. to load a sample, to
blocks
equilibrate a column etc. A block may contain other blocks or individual
instructions.
This is an example of text instructions in the Text pane:

How to save the A new method is untitled, and must be saved under a method name before it can
new method be used.
The table below describes how to save a new method.

Step Action
1 Click the Save Method toolbar or choose File:Save.

2 • If required, save the method in a folder other than the default

home folder.
• Enter a Method name for the method. The total path can be up
to 256 characters long. The method name must be unique for the
chosen system within the folder.

3 • If you have more than one system connected to the computer,

choose the System for which the method is intended. The method
can be run on any system that uses the same strategy. Remember
that different systems may have different configurations and
control capabilities.
• Choose the Technique for which the method was written.
• Click OK.

Result: The method is saved, but remains open in the Method Editor,
so that you can continue editing if you wish.

Note: You might want to sign your method. If you do so, you can choose to lock
the method so that nobody will be able to change the method. See 5.4 How to sign
the method on page 91 for further instructions.

• p 89
 5 How to create a method
5.3 How to use Text instructions

How to display A dedicated strategy is available for each system in the ÄKTAdesign platform.
descriptions of in- Although the majority of the instructions are general, some of them differ slightly
structions
between the individual strategies.
The list below describes two ways to display descriptions of the instructions in
your particular strategy:
• Select the instruction in the Instruction Box of the Method Editor and press <F1>

or
• Right-click the instruction in the Text pane and choose the menu option What’s
This?

How to print de- The table below describes how to print descriptions of the instructions in your
scriptions of in- particular strategy:
structions

Step Action
1 Select File:Print in the Method Editor.

2 • Select the Instruction set option to print the full set of instructions.
• Click OK.

How to add a The Snapshot instruction can be used to record the curve values at a specific point
Snapshot in the method run. For example, a snapshot can be inserted to record the curve
values immediately before an injection. The values are recorded in the result file
and can be viewed in the Snapshots tab of the Documentation dialog box (See 10.7
Run documentation on page 270). Up to 500 snapshots can be recorded in each
result file. The table below describes how to add a snapshot instruction to a method:

Step Action
1 • In the Text pane, select the instruction immediately before the
position where you want to insert the Snapshot instruction.

2 • Select Other in the Instructions field of the Instructions box.

• Select Snapshot in the instructions list.

3 Type a name in the Name text box in the Parameters field.

• Click the Insert button.

Note: Snapshots can also be taken in the System Control and Evaluation modules.
However, these snapshots will only record the data for a specific moment. For
more information about the Snapshot function see 2.2.7 Snapshots on page 41.

03-0014-90 • p 90
 How to create a method 5

5.4 How to sign the method

Instruction If you sign the method, you can choose to lock it so that nobody will be able to
change it.
The table below describes how to sign the method.

Step Action
1 Choose File:Sign Method in the Method Editor.
Result: The Sign the Method dialog box is displayed.

2 Click the Signing tab and do the following:

• Select a user in the User drop-down list box. In most instances,
you will want to use the current user shown on the list.
• In the Meaning field, provide a short text description explaining
the meaning behind the signature (for example “Method now
fully tested and approved”).
• Type your signature password in the Password field. If desired,
select the Lock box to lock the method permanently from further
changes by other users.
• If needed, view a list of all signatures associated with the current
method on the View Signatures tab.
• Click OK on either the Signing or View Signatures tab.

• p 91
 6 How to edit methods

6 How to edit methods

Introduction This chapter describes the complete facilities for editing methods in UNICORN.
For many applications, suitable methods can be created by changing the default
values in one of the wizard-generated methods supplied with UNICORN.
Use the more advanced editing facilities described here when you want
• to change selected instructions in the method, for example, change the outlet
valve position
• to add blocks and instructions
• to change method instructions to adapt to non-standard system configurations.

In this chapter This chapter contains these sections:

Topic See
The Method Editor interface 6.1

Method blocks 6.2

Method instructions 6.3

How to use method variables 6.4

Run Setup 6.5

How to use selected method instructions 6.6

Standard Watch conditions 6.7

How to save or delete a method template 6.8

How to print a method 6.9

How to export a method 6.10

03-0014-90 • p 92
 How to edit methods 6

6.1 The Method Editor interface

Introduction This section contains a general description of the Method Editor user interface and
the editing operations that can be performed in the different parts of the module.

In this section This section contains these topics:

Topic See
The Method Editor module 6.1.1

Text Instructions editor 6.1.2

• p 93
 6 How to edit methods
6.1 The Method Editor interface
6.1.1 Method Editor module

6.1.1 Method Editor module

Two modes The Method Editor interface operates in two modes:

• Text Instructions editor for entering and editing method instructions (see 6.1.2
Text Instructions editor on page 95)
• Run Setup for defining method properties (see 6.5 Run Setup on page 122).

How to open the The table below describes how to open the dialog boxes in the Method Editor:
Method Editor
dialog boxes
If you want to open... then...
the Text Instructions ed- click the Text Instructions icon.
itor

or
choose View:Text Instructions.

the Run Setup click the Run Setup icon.

or
choose View:Run Setup.

the Log Format click the Log Format icon.

or
choose View:Log Format.

the Method Wizard click the Method Wizard icon.

or
choose File:Method Wizard.

03-0014-90 • p 94
 How to edit methods 6

6.1.2 Text Instructions editor

How to select You have a choice of four panes that can be open together with the Instruction box
panes to be dis- in the Text Instructions editor, all at once or one at a time.
played
Follow the steps in this table to select the panes to be displayed:

Step Action
1 • In the Method Editor, choose View:Text Instructions

or
• click the Text Instructions icon.

2 • Choose View:Panes:Customize (or select additional panes here)

or
• click the Customize Panes icon.

3 Select panes
• Select panes in the dialog box and click the OK button.

Deselect panes
• Deselect panes in the Customize Panes dialog box and click the
OK button.

or
• right-click a window and select Hide.

• p 95
 6 How to edit methods
6.1 The Method Editor interface
6.1.2 Text Instructions editor

Method editing This table shows the method editing operations that can be performed in the
operations per- different panes:
formed in the dif-
ferent panes
The pane... Is used... See section
Text • to display instruc- 6.2.1 How to view
tions method blocks on page
• to display and hide 98
block instructions. 6.3 Method instructions
• to select current in- on page 111
struction.
• to edit instructions
• to cut, copy and
paste instructions.
• to move instructions
within a breakpoint.

Flow scheme • for information only. 9.2.4 The Flow Scheme

This window is not pane on page 204
updated according to
system status and
changes in the meth-
od.

Instruction box • to specify break- 6.3.2 How to add meth-

points, instructions, od instructions on page
parameters and vari- 113
ables.
• to insert, change and
delete instructions.

Block to select or display 6.2 Method blocks on

blocks. page 97

Gradient to display block dura- 6.5.5 The Gradient tab

tion and eluent gradient on page 133
throughout the method.

03-0014-90 • p 96
 How to edit methods 6

6.2 Method blocks

Introduction This section contains a description of how to organize a method in blocks of

instructions in order to make it more structured, and of how to work with method
blocks.

In this section This section contains these topics:

Topic See
How to view method blocks 6.2.1

How to call method blocks 6.2.2

How to add method blocks 6.2.3

How to delete method blocks 6.2.4

How to rename method blocks 6.2.5

How to find, copy and move method blocks 6.2.6

How to import method blocks 6.2.7

• p 97
 6 How to edit methods
6.2 Method blocks
6.2.1 How to view method blocks

6.2.1 How to view method blocks

Instructions can To view a method as a long list of individual text instructions can be confusing
be grouped into and inconvenient. Text instructions can therefore be grouped into blocks of
blocks
instructions that define a specific functional use. For example, one block might
contain the instructions necessary to equilibrate a column, and another block
contains instructions to load a sample, etc.

The Text pane In the Text pane of the Method Editor, the method is shown as a list of blocks,
denoted by the blue square symbols. Note that a block can also contain sub-blocks.
The figure below shows the text instructions in blocks:

The table below describes how to view or hide the instructions:

If you want... then...

to view the instructions click the “+” symbol
or
double-click the block name.

to hide the instructions click the “-” symbol

or
double-click the block name.

03-0014-90 • p 98
 How to edit methods 6

The Block pane The organization of blocks in the method is shown graphically in the Block pane
of the Method Editor.
Description
Each block is represented by a gray bar with the block name and the length of the
block. The line is shifted down to indicate calls to other blocks.
Click on the line that represents a block in the Block window to expand the block
in the Text pane and select the first instruction in the block.
Figure
The figure below is an example with a Watch instruction to start the fraction
collector which is active throughout the gradient elution block. Loop (to repeat a
group of instructions) and Hold_until instructions are also indicated in the Block
pane.

The Gradient Blocks are represented in the Gradient pane of the Method Editor by marks on the
pane X-axis. The marks show the length of each block. The name of the block in which
the cursor line is currently placed is shown at the top of the pane.
The figure below describes the Gradient pane:

• p 99
 6 How to edit methods
6.2 Method blocks
6.2.2 How to call method blocks

6.2.2 How to call method blocks

General descrip- To execute the instructions contained within a block in a method, the block must
tion be called by the program. When a block is called, the instructions in the block are
executed in the order that they are written until the block is finished or the
End_Block instruction is executed. Any settings made in a block are valid throughout
the method until the settings are changed.

Types of calls There are two types of calls:

• Unconditional calls, which are made with a Block instruction.
• Conditional calls, which are made with a Watch instruction. This makes it
possible to call a specified block or an instruction when a particular monitor
signal meets a given condition. As long as the condition is not met, the block is
not activated.

Watch instruc- Watch instructions are indicated by a green line that show the start and duration
tions of the watch. These instructions can use various conditions to respond to absolute
signal values or to rate of signal changes.
The breakpoint when the Watch instruction is issued determines when the watch
begins, not when the block is activated. Once set, a watch remains active until the
condition is met or a new Watch instruction is issued for the same monitor. The
watch is cancelled automatically when the condition is met. A watch can also be
turned off with the Watch_off instruction.
See F Method examples on page 545 for more details on Watch instructions.

03-0014-90 • p 100
 How to edit methods 6

6.2.3 How to add method blocks

Two ways to add You can add method blocks to a method in two ways, using either
method blocks
• the Instruction box of the Text Instructions editor,

or
• the New Block dialog box reached via the New Block icon.

Both these alternatives are described below.

How to add The table below describes how to add blocks with the Instruction box:
blocks with the
Instruction box
Step Action
1 In the Text pane of the Text Instructions editor, select the instruction
or block that you want to precede the new block.

2 Select Other:Block in the Instruction box.

3 • Enter a name for the block in the Block field.

• Click the Insert button.

Result: The block is inserted after the block that was selected in step
1.

The New Block The illustration below shows the New Block dialog box that can be used when
dialog box adding new method blocks:

• p 101
 6 How to edit methods
6.2 Method blocks
6.2.3 How to add method blocks

How to add The table below describes how to add blocks with the menu options of the New
blocks with the Block dialog box:
New Block dialog
box
Step Action
1 Choose Block:New in the Method Editor
or
click the New Block icon.

Result: The New Block dialog box is displayed.

2 Enter the relevant information in the New Block dialog box, and
click OK.
Result: The new block is added to the method, and placed last of all
blocks.
Note: The block can be placed in other positions by selecting some-
thing other than Main in the From droplist.

The fields of the The table below describes the fields of the New Block dialog box:
New Block dialog
box
Field Description
Name Block names can be up to 30 characters long, and can contain letters
(A-Z), digits (0-9) and the underscore character.
Block names must be unique within the method. The case of letters
is retained but not significant (the names Start_Frac and START_FRAC
are treated as identical).

Base One of the following options can be selected:

• SameAsMain: the new block will inherit the base from the Main
block in the method. The corresponding Base instruction will be
inserted in the block at breakpoint 0.
• Time: The block will be based on time.
• Volume: The block will be based on volume.
• Column volume: The block will be based on column volume.

Length A block continues until the breakpoint for the End_Block instruction
has been reached.
An End_Block instruction will automatically be inserted in the block
at the defined breakpoint. This field must not be left blank.

03-0014-90 • p 102
 How to edit methods 6

Field Description
Call You can call the new block from an existing block (for example the
Main block).
Select values in the two fields:
• From
The block from which the newly created block should be called.
• At
The breakpoint at which the call is to be made.

If you do not want to call the block (for example when the block
being created is to be activated by a Watch instruction), choose the
<Unused> line from the From drop-down list. Blocks using this line
are placed last in the method in the Unused category.
Note: You should not call a block from within itself. If you do, you
will generate a potentially infinite loop that exceeds the maximum
number of calls allowed in a method. A loop symbol is displayed at
the beginning of the line if this occurs.

• p 103
 6 How to edit methods
6.2 Method blocks
6.2.4 How to delete method blocks

6.2.4 How to delete method blocks

Four ways to de- There are four ways to delete blocks:

lete blocks
• To right-click a block and choose Delete from the shortcut menu
• To select a block and click Delete in the Instruction box
• To select a block and press the <Delete> key on the keyboard
• To select a block and use the Block:Delete Block command

Note: When you use any of the first three ways, the Method Editor dialog box will
give you the option to transfer the block to the Unused section.

Delete options The Delete Block dialog box is displayed when you delete a block with one of the
first three options mentioned above.

Options
Choose from the following options:
• Delete: The block is totally removed from the method. If the block is called
several times in the method, all the blocks will be deleted. Blocks deleted in this
fashion cannot be called again in the method.
Note: If the block contains sub-blocks, another dialog box is displayed, asking
you if you want to delete the sub-blocks as well.
• Move: The block is deleted from the method and transferred to the Unused
section. If the block is called several times in the method, however, only the row
with the block currently marked in the Text pane will be deleted. In this case,
the block will not be placed in the Unused section (since the block is still used
in the method). Blocks deleted in this fashion can be called again in the method.

03-0014-90 • p 104
 How to edit methods 6

How to use the The table below describes how to delete a block using the Block:Delete Block
Block:Delete command:
Block command

Step Action
1 Select the menu command Block:Delete Block in the Method Editor.
Result: The Delete Block dialog box is displayed with all blocks listed
in alphabetical order.

2 Select the blocks you want to delete and click OK.

3 Click Yes to confirm.

How to delete un- The table below describes how to delete an unused method block.
used blocks
Step Action
1 Highlight the method block.
• Press the <delete> key

or
• Right-click and choose Delete on the shortcut menu.

Result: The Delete Block dialog box opens. Note that the Move button
is not available.

2 Click the Delete button.

Result: The unused block is deleted and cannot be called upon again
in the method.

• p 105
 6 How to edit methods
6.2 Method blocks
6.2.5 How to rename method blocks

6.2.5 How to rename method blocks

Instruction The table below describes how to rename blocks:

Step Action
1 Right-click the block you want to rename in the Text pane and select
Rename.
Result: The Rename Block dialog box is displayed.

Note: By default, the block that is currently selected in the Text

window is automatically selected in the dialog box.

2 Enter the new name in the New name field and click Rename.

3 • If needed, repeat step 3 for other blocks.

• Click Close.

Note: If the block you renamed is called in a Block or Watch instruc-

tion, the block name in these instructions will be changed automat-
ically.

03-0014-90 • p 106
 How to edit methods 6

6.2.6 How to find, copy and move method blocks

Introduction By using the Edit options in the Method Editor, you can find, copy and paste and
move blocks within a method.

How to find text The table describes how to find text strings in the method text.
strings in the
method text
Step Action
1 Choose Edit:Find in the Method Editor,
or
right-click an instruction or a block in the Text window and select
Find.
Result: The Find dialog box is displayed.

2 • Enter the text you want to search for, search direction and case
matching criteria.
• Click OK.

How to copy and The table describes how to copy a block.

paste a block
Step Action
1 • Right-click the block you want to copy.
• Choose Copy.

2 • Right-click the instruction line just above the point where you
want the block to be pasted.
• Choose Paste.

Result: A dialog box asks if you wish to rename the pasted block.

3 Click Yes to rename the block before insertion, or No to insert the

copied block directly.
Result: The pasted block is inserted with the same breakpoint value
as the block or instruction selected for point of insertion.

• p 107
 6 How to edit methods
6.2 Method blocks
6.2.6 How to find, copy and move method blocks

How to move a The table describes how to move a block.

block
Step Action
1 • Right-click the block you want to move.
• Choose Cut.

2 • Right-click the instruction line just above the point where you
want the block to be pasted.
• Choose Paste.

Result: The block is now removed from its original breakpoint and
pasted at the new breakpoint. The pasted block is inserted with the
same breakpoint value as the block or instruction selected for point
of insertion.

03-0014-90 • p 108
 How to edit methods 6

6.2.7 How to import method blocks

Introduction You can import method blocks from other method files. You can also use this
function to copy blocks within a method. In the latter case, it is important to note
that it is the saved version of the method that will be copied, not changes that have
been made after you last saved the method.
The block is imported exactly as it appears in the source method. If the base of the
imported block is defined as SameAsMain, the block will inherit the main base in
the new method, regardless of the base in the source method. Also, the imported
block is inserted with the same breakpoint value as the block selected for point of
insertion.

Instruction The table below describes how to import method blocks:

Step Action
1 Choose Block:Import Block As in the Method Editor.
Result: The Import Block dialog box is displayed.

2 • Select the method from which you want to import a block.

• Select the block.

Result: The name of the selected block is displayed in the Block name
field.

• p 109
 6 How to edit methods
6.2 Method blocks
6.2.7 How to import method blocks

Step Action
3 In the Call field, do the following:
• On the From drop-down list, select a block into which the block
will be imported.
• In the At field, select the breakpoint value for the block to be im-
ported.

Click the Import button.

Note: The imported block cannot have the same name as an existing
block in the method. If the default name is not allowed for this
reason, the Import button will be gray and locked. If this occurs,
change the name of the imported block so that the Import button
becomes available.

4 • Repeat steps 2 and 3 if needed.

• Click the Close button.

03-0014-90 • p 110
 How to edit methods 6

6.3 Method instructions

Introduction This section describes how to work with the individual method instructions, in
order to edit method blocks and methods.

In this section This section contains these topics:

Topic See
How to read method instructions 6.3.1

How to add method instructions 6.3.2

How to delete method instructions 6.3.3

How to change or move method instructions 6.3.4

• p 111
 6 How to edit methods
6.3 Method instructions
6.3.1 How to read method instructions

6.3.1 How to read method instructions

Description of in- Method instructions are displayed in the Text pane of the Text Instructions Editor.
struction markings The table below explains the meaning of the markings:

Marking Explanation
Blue square beside text Valid call instructions, that is, Block and Watch in-
structions to other blocks in the method.

Blue square with a red Call instruction that contains one or more invalid
cross instructions.

Bold text Valid instructions.

Red dot Instructions with invalid syntax. All such instructions

must be deleted or changed before a method can be
run. See 6.3.4 How to change or move method instruc-
tions on page 115.
The instructions may be of the following types:
• Calls to blocks which are not defined in the
method
• Instructions that apply to a different system
strategy (can occur if a method is written for one
system and saved for another)
• Instructions for components that have not been
selected in the System Setup.

Normal text Instructions that will not be executed because

• they are positioned after the end of a block or
method
or
• they constitute a block to which there is no call.

Text with a loop sym- When a block is called from within itself this will
bol generate a potentially infinite loop, which might ex-
ceed the maximum number of calls allowed in a
method.

03-0014-90 • p 112
 How to edit methods 6

6.3.2 How to add method instructions

Instruction The table below describes how to add a method instruction in the Text Instructions
Editor:

Step Action
1 Select a block in the Text pane, and display the instructions within
the block.

2 Select an instruction line in the block. Make sure that the selected
instruction line is in the block, not the call to the block.

3 Open the Instruction box if it is not already displayed (View: Panes).

Do the following:
• Set the desired breakpoint in the Breakpoint field.
• Choose the instruction type and the instruction in the Instructions
field. For basic help on each instruction, click the instruction and
press <F1>.
• Type values for instruction parameters in the Parameters fields.
If a scroll bar appears at the right side of the Parameters field,
additional parameters are required.

4 Click the Insert button.

Result: The instruction will be inserted in the block
• at the position of the breakpoint of the new instruction, if there
are no other instructions at that breakpoint
• immediately after the currently highlighted instruction, if the
highlight is at the same breakpoint as the new instruction
• as the last instruction at the breakpoint, if there are several in-
structions at the same breakpoint and none of these is highlighted.

Note: Instructions that are placed at the same breakpoint are ex-
ecuted simultaneously, with the exception of Block instructions which
are executed in the sequence in which they are written.

Pause, Hold and If you use ÄKTA systems, the Pause, Hold, and Hold_until instructions will stop
Hold_until instruc- execution at this breakpoint, that is, instructions following after Pause, Hold and
tions
Hold_until at the same breakpoint will not be executed until a Continue instruction
is issued.

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 6 How to edit methods
6.3 Method instructions
6.3.3 How to delete method instructions

6.3.3 How to delete method instructions

Instruction The table below describes how to delete method instructions in the Text Instructions
Editor:

Step Action
1 Select the instruction in the Text pane.

2 Use one of the following alternatives:

• Right-click the instruction and choose Delete in the displayed
menu,
or
• press the Delete button in the Instruction box,
or
• press the Delete key on your keyboard.

End_Block instruction
If you delete the End_Block instruction, the block will end at the last instruction in
the block. If a gradient is currently being formed, the gradient will continue into
the next block.

How to suspend An instruction that has been deleted can only be recovered by re-inserting the
execution tempor- instruction. If you want to suspend execution of an instruction temporarily (for
arily
example during development work), you can replace the breakpoint with a value
after the End_Block or End_Method instruction.

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 How to edit methods 6

6.3.4 How to change or move method instructions

How to change an The table below describes how to change an instruction in the Text pane of the Text
instruction Instructions Editor:

Step Action
1 Select the instruction.
Result: The instruction with its current parameters is displayed in
the Instruction box.

2 Make the required changes to the breakpoint or parameters

or
select a new instruction in the Instruction Box.

3 Click the Change button

or
the Replace button.
Note: These buttons are equivalent unless changes are made to the
breakpoint or the length of a gradient. See below.

Effects of the The table below describes the difference in function between the Change button
Change button and the Replace button when you change breakpoints:
and the Replace
button on break-
points Button Function
Change This button shifts all subsequent instructions in the
block according to the change in the breakpoint.
Change does not affect the relative order of instruc-
tions in the method. You cannot change the break-
point of an instruction to earlier than the nearest
previous breakpoint in a block.
The illustration shows an example where Fractionation
is changed from breakpoint 0 to 5:

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 6 How to edit methods
6.3 Method instructions
6.3.4 How to change or move method instructions

Button Function
Replace This button moves the selected instruction but does
not change the breakpoint of any other instruction.
Replace can change the relative order of instructions
in the method.
The illustration shows an example where Fractionation
is changed from breakpoint 0 to 5:

Effects of the The Length parameter in the Gradient instruction affects the length of a gradient.
Change button Depending on which button you use, the change will have different results. The
and the Replace
button on gradi- table below describes this:
ent length
Command Function
Change If this button is used to change the length of a gradi-
ent, the breakpoints for any instructions issued during
the progress of the gradient will be adjusted propor-
tionately so that they are always placed at the same
relative position within the gradient. Instructions is-
sued after the end of the gradient will be shifted by
the amount of the change. Since the gradient works
over time, any instruction that you want to insert
after a gradient should be placed after the combined
breakpoint and gradient length.
Note: Moving the End_block instruction in a gradient
block with the Change button does not affect the
length of the gradient.

Replace If this button is used to change the length of a gradi-

ent, other instructions are not affected.

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 How to edit methods 6

Illustration of the The illustration shows the different effects of the Change button and the Replace
effects of the button on instructions within and after gradients:
Change button vs.
the Replace but-
ton on gradients

How to move an Move an instruction within the same breakpoint

instruction
Select the instruction in the Text pane of the Text Instructions Editor and drag it to
its new location to change the order of instructions within the same breakpoint in
a block.
Move an instruction to another breakpoint
The table below describes how to move an instruction to another breakpoint:

Step Action
1 • Select the instruction in the Text pane of the Text Instructions Editor.
• Choose Edit:Cut.

2 Select the instruction line just above the point where you want the
cut instruction to be pasted.
Choose Edit:Paste.
Result: The instruction is now removed from its original breakpoint
and pasted at the new breakpoint. The pasted instruction is inserted
with the same breakpoint value as the instruction selected for point
of insertion.

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 6 How to edit methods
6.4 How to use method variables

6.4 How to use method variables

Introduction Method variables can be used to edit suitable methods. Variables can be assigned
to most instruction parameters including breakpoints. Variables also form the
foundation for automatic method scouting.
Each parameter defined as a variable is also assigned a default value, which is used
if no changes are made to variable values at the start of a run. Up to 500 variables
can be defined in a single method.
All variables are listed on the Variables tab of the Run Setup, grouped according to
the block in which they appear. See 6.5.2 The Variables tab on page 125.

Identifying vari- Parameters defined as variables can be identified in two ways:

ables
• In the Text pane in Text instructions, the parameter is given as the default value
in parentheses followed by the variable name. The illustration below shows an
example of this:

• When the instruction is shown in the Instructions field of the Instruction box, the
VAR button beside the parameter field is displayed in capital letters, that is VAR
not Var.
The illustration below shows an example of the Instruction box where UV1 and
UV2 are defined as variables and the UV3 position is fixed.

When to change Variable values can be changed immediately before the start of a method run
variable values without using the Method Editor, allowing one method to be used for runs under a
variety of conditions.

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 How to edit methods 6

How to change To change default variable values, you can either

variable values
• edit the instruction in the Instruction box

or
• change the value in the Variables tab of Run Setup.

Changes made in the Text pane are automatically updated on the Variables tab and
vice versa.
The figure below illustrates the relationship between variables in the Text pane and
on the Variables tab of Run Setup:

Breakpoints or If a breakpoint or gradient length is defined as a variable, changing the variable

gradient lengths value in the Variables tab when the method run is started will shift other instruction
breakpoints accordingly. This functionality is equivalent to using the Change button
to alter a breakpoint or gradient length (see 6.3.4 How to change or move method
instructions on page 116 for how the Change button affects instructions within
gradients).

How to define Only one variable that affects block length (breakpoint or gradient length) may be
new variables defined within each block. However, any number of parameters may be defined
as variables within a block. The table below describes how to define a new variable.

Step Action
1 Select the instruction where you want to define the variable in the
Text pane of Text instructions.
Result: The parameters for the instruction are shown in the Instruction
box.

2 • Locate the breakpoint or the required parameter in the Instruction

box.
• Click the Var button.

Result: The Variable Name Definition dialog box opens.

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 6 How to edit methods
6.4 How to use method variables

Step Action
3 • Enter a name for the variable.
• Select the Visible in details only check box if you want to set the
variable as a "details" variable. Detail variables only become
visible on the Variables tab if the Show details check box is selec-
ted. This option is useful for hiding less important variables.
• Click OK.

Result: The Var button changes to VAR to confirm the new variable.
The variable is displayed in the Text pane.

Variable names Variables are defined with names that can be explicit descriptions of the variable
function, for example Sample_volume and Gradient_length. Suitable choices of
variable names can make the method easier to read and understand, and also help
the operator in setting variable values at the start of a method run.
The names can be up to 32 characters long and the following characters can be
used:
• Letters (A-Z)
• Digits (0-9)
• The underscore character (_)

The case of letters is retained, but not significant. The names Flow_Rate and
FLOW_RATE are treated as identical.

How to rename a The table below describes how to rename a variable:

variable
Step Action
1 Select the instruction that includes the variable you wish to rename
in the Text pane of Text instructions.
Result: The parameters for the instruction are shown in the Instruction
box.

2 • Locate the required parameter in the Parameters field.

• Click the VAR button.

3 Enter a new variable name in the dialog box and click OK.

Note: Variables can also be renamed in the Edit Variables dialog box in the Method
Editor. See 6.5.2 The Variables tab on page 126 for more information.

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 How to edit methods 6

How to remove a The table below describes how to remove a variable by converting it into a fixed
variable value:

Step Action
1 In the Text pane of Text instructions, select the instruction with the
variable you want to remove.
Result: The parameters for the instruction are shown in the Instruction
box.

2 • Locate the required parameter in the Parameters field.

• Click the VAR button.

3 • Click the Clear button to delete the variable.

• Click OK.

Result: The VAR button changes to Var to confirm that the variable
is removed.

Note: Variables can also be deleted in the Edit Variables dialog box in the Method
Editor. See 6.5.2 The Variables tab on page 126 for more information.

• p 121
 6 How to edit methods
6.5 Run Setup

6.5 Run Setup

Introduction Run Setup is a part of the Method Editor. It has several tabs for defining method
properties. This section describes how to use the tabs and the information displayed
on the tabs.

In this section This section contains these topics:

Topic See
Overview of Run Setup 6.5.1

The Variables tab 6.5.2

The Scouting tab 6.5.3

The Questions tab 6.5.4

The Gradient tab 6.5.5

The Notes tab 6.5.6

The Evaluation Procedures tab 6.5.7

The Reference Curves tab 6.5.8

The Columns tab 6.5.9

The BufferPrep tab 6.5.10

The Method Information tab 6.5.11

The Result Name tab 6.5.12

The Frac-950 tab 6.5.13

The Start Protocol tab 6.5.14

How to export the values in the Run Setup 6.5.15

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 How to edit methods 6

6.5.1 Overview of Run Setup

Introduction To access Run Setup, either

• Click the Run Setup icon on the Method Editor toolbar,

or
• Select View: Run Setup.

Illustration of Run The illustration below shows an example of the Run Setup with the Variables tab
Setup selected:

The tabs The table below contains brief descriptions of the tabs of Run Setup. If you want
more detailed descriptions, see sections on the respective tabs:

Tab This tab...

Frac-950 allows the user to choose rack type and the fraction-
ation order for the Frac-950 fraction collector.

Variables lists all variables used in the method with their default
values, organized by method block.

Scouting shows the scouting scheme used for the method. The
scouting scheme can also be set up from this tab.

Notes shows the descriptive comments that form a part of

the method documentation.

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 6 How to edit methods
6.5 Run Setup
6.5.1 Overview of Run Setup

Tab This tab...

Gradient provides a graphical overview of the block structure
and eluent gradient tab in the current method.

BufferPrep displays information about the selected buffer prepar-

ation recipe for the current method.

Columns displays the columns used in the current method.

Reference curves displays the reference curves that will appear in the
System Control curve dialog box during the run of the
current method.

Evaluation Procedures shows the evaluation procedures that will run at the
end of the current method.

Method Information displays information about the method, such as

method name, target system, and last date of change.

Start Protocol determines which items of the Run Setup that are dis-
played at the start of the run.

Questions displays the questions used in the method. Questions

provide a means for entering run-specific information
at the start of a run. Use this tab when you want to
define questions.

Result name specifies how the result files will be named for the
results of a run, and where the result file will be saved.

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 How to edit methods 6

6.5.2 The Variables tab

Introduction The Variables tab lists all variables used in the method with their default values,
organized by method block. You can change the default values to create a variant
of the method.
Note: The variables of a block are only displayed once on the Variables tab, even
if the block is called several times in a method. Variables are displayed only if the
method contains variables.

Check boxes There are three check boxes on the Variables tab. The table below describes these
boxes:

Check box Select this box if you want...

Show details detail variables to be shown. Detail variables are in-
dicated by a D in the column immediately to the left
of the Variable column.

Show unused variables unused variables to be shown. Unused variables are

indicated by a U in the column immediately to the
left of the Variable column.

Display tooltip for exten- to display useful tips when you move the cursor to
ded variable cells fields that can have several functions.

Note: The options to show detail and unused variables can be set up as default
options in the Administration:Change User Attributes settings in the UNICORN Manager.

How to change Enter new values in the appropriate fields to change the default variable values.
the default values For some variables, pre-set values are available on drop-down menus. Save the
method when you have made your changes.
Note: The Variables box must be selected on the Start Protocol tab if you want to
be able to change variable values at the start of a method.

Blue values For variables with values shown in blue, the value input can be toggled between
OFF, INFINITE or other single position values, and a variable range. To change the
value, right-click the value cell.

Variables can also Variables can be changed in the Text Instructions Editor as well as on the Variables
be changed in the tab of the Method Editor. Changed values will be displayed for the corresponding
Text Instructions
Editor instructions in both windows.

• p 125
 6 How to edit methods
6.5 Run Setup
6.5.2 The Variables tab

How to delete or The table below describes how to delete or rename a variable in the Run Setup.
rename variables
Step Action
1 • Click the Edit Variable... button on the Run Setup Variables tab.

or
• Choose the Edit:Variable...Method Editor menu option.

Result: The Edit Variables dialog box opens. The variables are listed
alphabetically.

2 Select the variable to edit.

3 Rename
• Type a new variable name in the New name text box.
• Click the Rename button.

Result: The variable is renamed.

Delete
• Click the Delete button.
• Confirm that you want to delete the variable.

Result: The variable is deleted.

How to change a Detail variables are only shown if the Show details checkbox is selected on the
variable into a de- Variables tab. The table below describes how to set up a detail variable.
tail variable

Step Action
1 • Click the Edit Variable... button on the Run Setup Variables tab.

or
• Choose the Edit:Variable...Method Editor menu option.

Result: The Edit Variables dialog box opens. The variables are listed
alphabetically.

2 Select the variable to be changed.

3 • Select the Set visible in details only checkbox.

• Click the Close button.

Result: The variable is marked by the detail indicator D.

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 How to edit methods 6

How to change a The table below describes how to change a detail variable into a regular variable.
detail variable into
a regular variable
Step Action
1 • Click the Edit Variable... button on the Run Setup Variables tab.

or
• Choose the Edit:Variable...Method Editor menu option.

Result: The Edit Variables dialog box opens. The variables are listed
alphabetically.

2 Select the variable to be changed.

3 • De-select the Set visible in details only checkbox.

• Click the Close button.

Result: The detail variable indicator D is removed.

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 6 How to edit methods
6.5 Run Setup
6.5.3 The Scouting tab

6.5.3 The Scouting tab

Introduction A scouting scheme is a series of runs where chosen variable values are varied. You
can define up to 99 runs in a scouting scheme. When a method is run with scouting,
the method is automatically repeated for each selected run in the scouting scheme.
Typically, scouting will vary one or more variables in a series of runs, for example,
flow rate or elution gradient. See 7 Scouting on page 175 for instructions on how
to set up a scouting scheme, and 9.4 How to perform a scouting run. on page 216
Note: The Scouting tab is available only if the method contains variables.

Example of a The illustration below shows a scouting scheme for six flow rates and different pH
scouting scheme values:

Note: The Edit Variable... button on the Scouting tab opens the same Edit Variables
dialog box that can be accessed from the Variables tab.

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 How to edit methods 6

6.5.4 The Questions tab

Introduction The Questions tab of Run Setup is used for viewing and adding questions that the
system asks a user at the start of a run. These questions provide a means for entering
structured run-specific information. Method wizards and templates supplied with
UNICORN are defined with a set of questions for sample, column and eluent
identification.
Note: For questions to be shown in the start protocol, the Questions option must
be checked on the Start Protocol tab of Run Setup.

Question status Different types of questions have different status. The illustration below shows the
Question field, an example of a question and the status alternatives that can be
used:

The table below explains the different alternatives:

Question status Explanation

Mandatory These questions must be answered before a method
is started.

Authorized These questions must be signed with the users signa-

ture password to unlock and continue the method.

Chromatogram These questions will be printed with the answers on

the same page as the chromatogram, if a question is
chosen in an evaluation report.

Answer type A question has to be defined to accept one of four types of answers. The illustration
below shows an example where the Value option has been selected. The appearance
of the box to the right of the Answer type field depends on the answer type option
selected:

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 6 How to edit methods
6.5 Run Setup
6.5.4 The Questions tab

The table below describes the different answer types:

Answer type This option...

Input field accepts any alphanumerical input as the answer. Input
field questions may have a default answer.

Multiple choice allows the user to choose one of a defined set of an-
swers. To allow a blank answer, enter a space in one
of the predefined answers.

NoAnswer is used to
• display important information
or
• to split a question over more than one line by set-
ting all but the last line in a question to No answer.
(Normally, each question consists of one line only.)

It is impossible to give an answer to questions with

this option selected.

Value accepts only numerical answers. Value questions must

have specified maximum and minimum limits, and
may be defined to accept only integer values.

How to insert a The table below describes how to insert a question:

question
Step Action
1 If there are questions on the list, select the question that should be
followed by the new question.

2 Enter the question text, status, answer type and answer option as
required.

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 How to edit methods 6

Step Action
3 The Answer type determines what is displayed in the question defin-
ition field to the right of the Answer type field. For each answer type,
do as follows:
Input field
Enter a default answer if required.
Multiple choice
• Click in the text field under Alternatives.
• Enter the answer.
• Click the Add/Delete button.
Result: The new alternative is added at the end of the list.
• Repeat this procedure to add new alternatives. To remove an al-
ternative, mark the alternative in the scroll list and click the
Add/Delete button.

No answer
No action taken.
Value
Enter maximum and minimum limits. Select the Integer box if the
question is to accept integers only as answers.

4 Click the Insert button.

Result: The new question is added to the list.

How to preview The table below describes how to preview the questions as they will appear in the
questions Start Protocol.

Step Action
1 • Select a question.
• Click the Preview button.

Result: The question is displayed.

2 Click the Edit button to return to the question editing mode.

How to edit a The table below describes how to edit a question:

question
Step Action
1 Select the question you want to edit.

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 6 How to edit methods
6.5 Run Setup
6.5.4 The Questions tab

Step Action
2 Change the text, status, type and answer as required

3 Click the Replace button.

How to delete a Do one of the following to delete a question:

question
• Select a question and click the Delete button to remove the selected question.
• Click the Delete all button to delete all questions.

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 How to edit methods 6

6.5.5 The Gradient tab

Introduction The Gradient tab provides a graphical overview of the block structure and eluent
gradient in the current method. The description of this tab can also serve as a
description of the Gradient pane of the Text Instructions.
Note: For scouting runs, click Run X to see the gradient for each run.

Illustration The illustration below shows an example of a Gradient tab:

How to zoom in The table below describes how to zoom in on a selected area of the Gradient tab:
on a selected re-
gion
Step Action
1 • Press and hold the left mouse button and drag a rectangle on the
screen to select the area you want to zoom in on.
• Release the mouse button.

Result: The display is now zoomed in on the selected area.

2 Repeat the process for further magnification of selected areas.

How to reduce the To reduce the scale of the zoom function, right-click the tab and choose either:
scale of the zoom
function • Undo Zoom to reverse each zoom-in action a step at a time, or
• Reset Zoom to reverse all of the zoom-in actions to the default scale setting.

• p 133
 6 How to edit methods
6.5 Run Setup
6.5.5 The Gradient tab

How to use the A vertical marker line can be dragged from the Y-axis with the mouse. As you drag
vertical marker the marker line, the current position is identified at the top of the tab in terms of
line
the block name, X-position in the currently displayed base and eluent concentration
in per cent of eluent B.

How to change You can change the base shown on the Gradient X-axis. The alternatives are time,
the base shown on volume and column volumes. Changing the base for the display does not affect the
the X-axis
base in the method instructions, which means that you can check how long a
method will take simply by setting the axis scale to time, even if the method blocks
are written in volume or column volume base.
The list below describes two ways to change the base shown on the X-axis:
• Click the X-axis to toggle between the base types.

or
• Right-click anywhere on the Gradient tab.
Result: A sub-menu is displayed.
• Select Base and make the appropriate choice: Time, Volume or CV.

How to view You can display a hatched background on the Gradient tab. The table below
hatch marks describes how to do this:

Step Action
1 Right-click anywhere on the Gradient tab.
Result: A sub-menu is displayed.

2 Select Hatch.
Result: The Gradient background is hatched.

3 To hide the hatch marks, repeat steps 1 and 2.

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 How to edit methods 6

6.5.6 The Notes tab

Introduction Notes are descriptive comments that form part of the method documentation.
Method templates are supplied with notes describing the system requirements for
running the method. Read through these notes carefully before using a method.

Sub-tabs There are four sub-tabs:

• Method Notes
• Start Notes
• Run Notes
• Evaluation Notes

Only the Method Notes can be edited from the Method Editor; the other notes are
accessible at the respective stages in a run.

Recommended us- We recommend that you use Method Notes to describe the system setup required
age by the method (for example eluent and sample inlets, outlets and column
connections).
Use the Start Notes or Run Notes for run-specific information.
Note: Method Notes are saved with the method and apply to all runs made with
the method.

How to write To write method notes in your own methods, place the cursor in the white area of
method notes the Notes tab and type the relevant text. Use standard Windows editing functions
to edit the notes.

How to search for You can search for text strings in the method notes. The table below describes how
text strings to perform a search.

Step Action
1 Click the Find button.
Result: The Find dialog box opens.

2 • Type the text string in the Find what text box.

• Select search criteria and click OK.

Result: The located text string is highlighted in the text area.

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 6 How to edit methods
6.5 Run Setup
6.5.7 The Evaluation Procedures tab

6.5.7 The Evaluation Procedures tab

Introduction The Evaluation Procedures tab lists all evaluation procedures associated with the
method. Evaluation procedures can be called automatically at the end of a method
to evaluate and/or print the results.
Many UNICORN strategies are supplied with method templates or wizards that
include a number of evaluation procedures. User defined procedures are created
in the evaluation module and can be saved in method files (see 12.3 Automated
evaluations procedures on page 378).

Changes in the A procedure in a method will not be updated when a procedure with the same
Evaluation mod- name is changed in the Evaluation module. The same applies to report formats
ule
saved in a procedure.

References to Evaluation procedures that process chromatogram data rely on consistent

curves identification of curves in the result file for correct operation. If you include
evaluation procedures with a method, make sure that references to curves in the
procedure will be valid when the procedure is executed at the end of the run (see
12.3 Automated evaluation procedures on page 378 for more details).

How to print eval- If you use an evaluation procedure to print results automatically from a run
uation results controlled from a remote station in a network installation, the results will be printed
on the printer currently set up on the local station, not on the remote station.
If you execute the procedure interactively from the Evaluation module on the remote
station, the results will be printed on the printer set up on the remote station where
you are working.

How to define and Evaluation procedures are defined in the Evaluation module.
view evaluation
procedures Procedures imported to a method can also be viewed and edited in the Method
Editor. To do this, select the required procedure on the list and click the Edit button.

How to select pro- To select procedures to run, select the procedure(s) that are to be executed at the
cedures to run end of the run. The procedures will be executed in the order they appear on the
list.

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 How to edit methods 6

How to import The table describes how to import global evaluation procedures:
evaluation proced-
ures Note: Procedures saved with one method file can be imported to another.

Step Action
1 Select the Evaluation Procedures tab and click the Import button.
Result: The Import dialog box is displayed.

2 Choose either option 1 or 2 below.

Option 1: Select a global UNICORN procedure
1. Select a procedure on the Select list.
Result: The evaluation procedure name is displayed in the Import
as field.

Option 2: Select a procedure from another method

1. Select a method, that contains a procedure, in the left part of the
dialog box.
Result: The procedures of the selected method will be displayed
on the Select list.
2. Select the desired procedure on the Select list.
Result: The method name is displayed in the Import as field.

Note: Click Procedure List to return to the list of UNICORN’s

global evaluation procedures.

3 If desired, change the procedure name in the Import as field.

Note: The imported evaluation procedure cannot have the same
name as an existing evaluation procedure in the method. If the default
name is not allowed for this reason, the Import button will be gray
and disabled. When you change the name in the Import as field, the
button will become available again.

4 Click the Import button.

Result: The evaluation procedure is imported into the method.

5 Repeat steps 2 - 4 until you have imported all procedures.

6 Click the Close button.

How to delete The table describes how to delete evaluation procedures from the method:
evaluation proced-
ures
Step Action
1 Select the Evaluation Procedures tab.

2 Select the procedure(s) that you want to delete.

• p 137
 6 How to edit methods
6.5 Run Setup
6.5.7 The Evaluation Procedures tab

Step Action
3 Click the Delete button and confirm the deletion when prompted.
Result: The deleted procedures are immediately removed from the
method file.

How to rename The table describes how to rename evaluation procedures in a method.
evaluation proced-
ures
Step Action
1 Select the Evaluation Procedures tab and click the Rename button.
Result: The Rename dialog box is displayed.

2 • Select a procedure from the list and change the name in the Re-
name item to field.
• Click Rename.

3 Repeat step 2 until you have renamed all procedures required.

4 Click the Close button.

How to edit an The table describes how to edit evaluation procedures in a specific method:
evaluation proced-
ure
Step Action
1 Select a procedure on the Evaluation Procedures tab and click the
Edit button.
Result: The Procedure Editor dialog box is displayed, with information
about the selected procedure.

2 Enter the new parameter values in the appropriate place of the

Parameters field, and click the Replace button.
Result: The selected instruction in the evaluation procedure is up-
dated in accordance with the new parameters assigned to it.

3 If needed, insert new instructions after the currently selected proced-

ure instruction. Do the following:
1. Select an instruction type and instruction in the Instructions field.
2. Enter the appropriate parameter values in the Parameters field.
3. Click the Insert button.

Result: The new instruction is added to the evaluation procedure.

4 To remove an instruction from the evaluation procedure, select it

and click the Delete button.

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 How to edit methods 6

Step Action
5 Select File:Save as to save the edited procedure with a new name.
Click the Close button.

6 Select File:Close from the menu in the Procedure Editor dialog box.
Result: The Procedure Editor dialog box is closed and the procedure
is saved automatically.

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 6 How to edit methods
6.5 Run Setup
6.5.8 The Reference Curves tab

6.5.8 The Reference Curves tab

Introduction Reference curves are curves from existing result files that you can display in the
Curves pane of System Control during a run.

How to choose You can include up to five reference curves in a method. You choose which curves
and display refer- to display during the run with the View:Properties:Curves command in System
ence curves
Control (see 9.2.3 The Curves pane on page 199). Reference curves are only displayed
during the run. Reference curves are not saved in the result file.

How to add refer- The table below describes how to add a reference curve from a result file:
ence curves
Step Action
1 Select the Reference Curves tab and click the Import button.
Result: The Import Reference Curve dialog is displayed.

2 • In the left field, select the result file containing the curve to be
added.

Result: The Select list displays the available curves for the result file.
• Select the curve you want to add from the Select list.

3 • If desired, change the curve name in the Import as field.

Note: The curve name has to be changed if a reference curve with

that name already exists.
• Click Import.

4 Repeat steps 2 and 3 if you want to add more curves.

5 Click the Close button to close the Import Reference Curve dialog
box.

How to delete ref- The table describes how to delete reference curves.
erence curves
Step Action
1 Select the curves you want to delete.

2 Click the Delete button and confirm the action when prompted.

Note: Deleting curves from the method does not affect the curves in the result file
from which they were imported.

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 How to edit methods 6

How to rename The table below describes how to rename a reference curve in a method:
reference curves
Step Action
1 Click the Rename button.

2 • Select a curve from the list.

• Change the name in the Rename item to field.
• Click the Rename button.

3 Repeat steps 2 and 3 if you want to rename more reference curves.

4 Click the Close button.

Result: The reference curve name is changed.

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 6 How to edit methods
6.5 Run Setup
6.5.9 The Columns tab

6.5.9 The Columns tab

Display of the The Columns tab shows the parameters of the column selected for your method.
column paramet- The column parameters are displayed in the Column Data field. If you perform
ers
scouting runs with different columns, all of these will be listed. Select the appropriate
column to display the parameters.

Illustration The illustration shows an example of the Columns tab:

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 How to edit methods 6

6.5.10 The BufferPrep tab

BufferPrep usage BufferPrep allows a buffer of different pH and salt concentrations to be prepared
on-line from four stock solutions. This removes the need to manually prepare new
buffers every time the pH needs to be changed. Linear and step salt gradients can
be run and pH can be used as a variable scouting parameter. BufferPrep is optimized
for cation and anion exchange chromatography. For a complete description of
BufferPrep, see the user manual for ÄKTAdesign systems.
Note: BufferPrep is only available for some ÄKTAdesign systems.

Illustration The illustration below shows an example of the BufferPrep tab:

Stock solutions The solutions and the inlets to which they should be connected are displayed to
the right of the dialog box. Accuracy of preparation is essential. The four stock
solutions consist of:
• a mix of buffering components (there can be up to five different buffering
components enabling a broad pH range to be covered),
• an acid (HCl) or base (NaOH) for pH on-line titration,
• distilled water,
• an inert salt (for example NaCl) for salt gradient formation.

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 6 How to edit methods
6.5 Run Setup
6.5.10 The BufferPrep tab

How to create a If a suitable template or wizard is not available, you can create a BufferPrep method
BufferPrep meth- yourself. The instruction BufferPrep_pH must be available at breakpoint zero at the
od
beginning of the method. The method must not contain the instructions PumpAInlet
or PumpBInlet.
The table shows one way to create a BufferPrep method.

Step Action
1 In the Text Instruction editor:
• Insert a BufferPrep-pH block at breakpoint zero at the beginning
of the method.
• Define BufferPrep-pH as a variable.

1 Change to the Run Setup.

• Select the BufferPrep tab.
• Click the ON radio button in the Status field.
• Select a Recipe from the drop-down list box. There are two main
alternatives:
- AIEX or CIEX, which are recipes covering a broad pH range,
- single buffer recipes for more narrow pH ranges.

Result: All information relevant to the selected recipe will be dis-

played on the tab.

2 Prepare the required stock solutions.

3 Do one of the following:

• Select the Variables tab. Set the required pH for the method run
in the variable BufferPrep_pH,
or
• If you want to perform pH scouting, click the Scouting tab and
select BufferPrep_pH as a scouting variable. Enter the pH values
for the different runs.

BufferPrep recipes
The recipe saved in the method (the one selected on the BufferPrep tab) cannot be
edited, although fine tuning is possible. However, the recipes on the list of all
BufferPrep recipes can be edited. New recipes can also be created (see E How to
create and edit BufferPrep recipes on page 536).

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 How to edit methods 6

How to fine tune In order to obtain high pH accuracy, the recipe can be fine tuned around a specific
the BufferPrep re- pH by setting correction factors. The table below describes how to fine tune the
cipe with correc-
tion factors recipe with correction factors:

Step Action
1 In System Control, select Manual: Other.

2 Select BufferPrep Recipe and Recipe Name.

Click the Execute button.

3 • Set the pH in the instruction BufferPrep_pH in group Pump.

• Click the Execute button.

4 • Set the flow rate to be used during the run in the Flow instruction.
• Click the Execute button.

5 Check the pH reading when stable. Allow at least 30 ml of eluent

to pass through before expecting a steady pH reading.

6 • Change to 100% B by setting the Gradient instruction in Manu-

al:Pump to 100% for Target and 0 for Length.
• Click the Execute button.

7 Check the pH reading when stable at 100% B.

8 • If the readings are acceptable at both 0% and 100%, the correc-

tion factors do not need to be changed.
• If the readings are not acceptable, click the Corr. Factors button
in the BufferPrep tab in the method.

9 Enter the deviation at 0% and 100%. (e.g., if the pH is set to 7.0

and the actual pH is 7.1 enter 0.1. Enter -0.1 if the pH is 6.9).
Note: If correction factors already exist, the measured pH deviation
should be added to the old factors.

10 Save the method.

Note: When changing the correction factors for the recipe selected in the method,
the recipe with the same name on the list of all BufferPrep recipes is not affected.
The changes will only apply in the specific method.

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 6 How to edit methods
6.5 Run Setup
6.5.11 The Method Information tab

6.5.11 The Method Information tab

Introduction The Method Information tab displays information about the method. This tab is for
information only and cannot be edited.
There are three sub-tabs on this tab: Information, Signatures, and Method duration.

The Information The Information sub-tab displays

sub-tab
• method information such as method name, creation date, creator and date of
last change,
• target system,
• strategy information such as strategy name, date and size.

The Strategy Notes button displays what systems, programs and file versions the
strategy is designed for.

The Signatures The Signatures sub-tab has five information fields for all signatures. The table
sub-tab below describes the content of each field:

Field Description
Date Date of the signature.

Meaning Short description explaining the meaning behind the

signature.

User Name User name of the user who signed the method.

Full Name Full name of the user who signed the method.

Position Position of the user.

The Method Dura- The Method Duration sub-tab presents

tion sub-tab
• the estimated total time
• the estimated buffer volume required for the method.

If the method includes a scouting scheme, click the Run ’x’ button to see values for
the different scouting runs.

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 How to edit methods 6

6.5.12 The Result Name tab

Introduction The Result Name tab is used to specify:

• how the result files will be named for the results of a run
• where the result file will be saved
• the name of the special scouting folder where results from scouting runs will be
stored.

Illustration The illustration below shows an example of the Result Name tab:

Construction of The result file name is constructed by one of the base options listed below. The
the result file serial number is changed automatically each time the method is run.
name
Base options of the result file name are:
• The Method name plus a 3-digit serial number,
• The Date of the run (in an 8-digit format determined by the country setting in
Windows 2000 or XP) plus a 3-digit serial number,
• A freely specified Name (within the file naming restrictions of the operating
system) plus a 3-digit serial number.
• A selected Variable (from the droplist) plus a 3-digit serial number.

Note: If a result names includes decimal points (e.g. numeric variables) or underscore
characters, these characters will automatically be replaced by spaces. Points and
underscores are not allowed in the result names.

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 6 How to edit methods
6.5 Run Setup
6.5.12 The Result Name tab

Serial numbers If the result file folder already contains files with the same file name base, the serial
and unique identi- number is changed automatically. For scouting runs, the 3-digit serial number will
fiers
be the number of the executed run column in the scouting scheme.
A unique identifier can also be generated automatically, in addition to the serial
number. The identifier is a string of numbers inserted between the result file name
and the three-digit serial number.
• Select Add unique identifier to result name in the Result name field.

Batch ID for each UNICORN will automatically issue a Batch ID to each method run. This ID is
test run displayed before the Base in the logbook and can be used to identify individual
runs. See illustration in 9.2.5 The Logbook pane on page 205. If Changeable batch
ID is selected, another ID string can be typed in the Start Protocol.

Specify result The result name can be specified as changeable in the Start Protocol (see 6.5.14 The
name as change- Start Protocol tab on page 152). In that case, the information you supply on the
able
Result Name tab will be the suggested result name, but you can change this at the
start of the run.

How to save the By default, result files are stored in the home folder of the user who starts the run.
result files in a dif- The table below describes how to change the folder where the result file will be
ferent folder
stored:

Step Action
1 If the run contains information that is not important, you can save
disk space by selecting the No result check box, thereby storing the
result in the Temporary folder (named Manual Runs, where only the
latest 10 result files are saved).
If not, go to step 2.

2 Click the Browse button.

3 • Double-click the required folder icon.

• Click the OK button.

How to save Scouting results will be saved in a special folder as specified by the result file path.
scouting results To select a folder, type a name for the folder in the Scouting subdirectory field.
Each time the scouting method is run, a new folder will be created with the name
and a serial number (entering IEXSC will create folders IEXSC001, IEXSC002,
etc.).

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 How to edit methods 6

6.5.13 The Frac-950 tab

Introduction The Frac-950 tab is used for defining options for Frac-950. The user can choose
rack type and fractionation order.

How to set up The table describes how to set up the fractionation.

fractionation
Step Action
1 Select rack type from the Rack drop-down list.

2 Select the order for fractionation by using the Fraction order radio
buttons.

Manual runs In System Control, for manual runs, the Frac-950 tab cannot be used. Instead, use
the manual fractionation instructions, starting with Man_.
• Choose Manual:Frac to open the Frac Instructions dialog box.

Total number of The total number of tubes sampled may differ if a last tube has been chosen. The
tubes Number of tubes equation in the bottom left corner of the Frac-950 dialog box of
the Start Protocol shows the current number of available tubes chosen for
fractionation, followed by the total possible number in parentheses.

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 6 How to edit methods
6.5 Run Setup
6.5.13 The Frac-950 tab

How to select the You can select a position for the last tube to be used in the fractionation process.
last tube If the process attempts to go further than the selected last tube during a method
run, an alarm will be executed.
The last tube position can only be selected on the Frac-950 dialog box in the Start
Protocol when you start a method run, or when you do an instant run. The
illustration below shows an example of this dialog box:

The lower right box within the Last tube field shows the currently selected last tube.
The table below describes how to re-define the last tube:

Step Action
1 In the Frac-950 dialog box, select the Define box in the Last tube field
to select the last tube position.

2 Place the cursor over the appropriate tube (circle) within the tube
matrix and click again.

Note: When using different sized tubes in the same rack, the last tube can be set
for both tube sizes. Use the Tube type drop-down list to choose the desired tube
size, and then follow the procedures outlined above to select the last tube.

How to set the If you want to return to the default last tube position, click the Reset to default
last tube to default button of the Frac-950 dialog box in the Start Protocol when you start a method
setting
run.

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 How to edit methods 6

6.5.14 The Start Protocol tab

Introduction The Start Protocol tab determines which items of the Run Setup are displayed at the
start of a method run. Click the Start Protocol tab and select the items that you
want to be displayed.

Checkboxes The table below describes the check boxes of the Start Protocol tab:

Checkbox Displays...
Frac-950 the Frac-950 setup parameters, which can be changed.

Variables values for method variables that can be changed at

the start of the run.
These values will override the default values for the
particular run and be saved in the result file. The de-
fault values stored in the method are not affected.

Scouting the scouting scheme which can be changed at the start

of the run. Changes will override the default settings
and values for the particular run and be saved in the
result file.

Text Method method instructions. They cannot be changed from

this display.

Notes the Notes tab.

Gradient the gradient.

BufferPrep the recipe selected in the method. The recipe cannot

be changed during the start of a run.

Columns the available column definitions.

Reference curves the reference curves associated with the method.

Evaluation procedures the evaluation procedures set to be executed at the

end of the method.

Method information the method information.

Settings the settings.

Calibration the monitor calibration settings.

Questions questions defined in the method.You are recommen-

ded to always use this option, since the answers to
questions can form an important part of the
UNICORN run documentation.

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 6 How to edit methods
6.5 Run Setup
6.5.14 The Start Protocol tab

Checkbox Displays...
Result name the result name, which is changeable if this option
has been selected. Click the Browse button to change
the result folder.
If the box is not selected, the result name will still be
displayed, but you will not be able to change the name
or folder.

Scouting start pro- The table below describes the options in the Scouting start protocol field.
tocol field
Option If you check this option...
First run only parameters for the scouting runs can be adjusted at
the beginning of the first run only. After that, the runs
will be performed automatically without operator
intervention.

All runs the Scouting start protocol will be displayed at the

beginning of each run in the scouting scheme.

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 How to edit methods 6

6.5.15 How to export the values in the Run Setup

Instruction You can easily export the values in the Run Setup to a file, and save it in ASCII
format. This is useful when you want to enable others to read the methods without
having access to UNICORN on their computers.
The table below describes how to export the values in the Run Setup and save them
to a file.

Step Action
1 In the Text instructions Editor or the Run Setup, select File:Export:Run
Setup.
Result: The Export Run Setup dialog box is displayed.

2 • Select the boxes to select the parts of Run Setup that you want to
export.
• Click the Export button.

Result: The Export dialog box is displayed.

3 • Type a file name and select the target drive and folder.
• Click the Save button.

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 6 How to edit methods
6.6 How to use selected method instructions

6.6 How to use selected method instructions

Introduction This section provides recommendations for how to use some common programming
features in UNICORN methods. They are available from the Instruction box in the
Method Editor.

In this section This section contains these topics:

Topic See
Base instruction 6.6.1

Instructions at the same breakpoint 6.6.2

Block and method length 6.6.3

Messages and Set_Marks 6.6.4

How to delay a method 6.6.5

Linear flow rates 6.6.6

Gradients and eluent concentrations 6.6.7

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 How to edit methods 6

6.6.1 Base instruction

Bases Every method block must start with a Base instruction, defining the base for
calculating breakpoints.
Different blocks can use different bases. The base can be one of the following:
• volume (the unit depends on the scale defined in the system strategy)
• time (minutes)
• column volume, CV (defined as a numerical value or taken from the column
definition)
• SameAsMain (all blocks apart from the main block), which means that the block
will inherit the base defined in the main block.

Method blocks that use a volume or column volume base

Make sure that the flow rate is not zero. Volume breakpoints are calculated from
the flow rate of the pump, and the method will not progress if the flow rate is zero.

What base should Use the base that most closely suits the purpose of the block. Column volume is
I use? recommended as the base for most steps in a run. In some situations, however, it
may be more suitable to use a time or volume base for individual blocks.
To change the base for an existing method
Be careful when changing the base for an existing method. Changing between time
and volume bases can affect the relative duration of steps in the method if different
steps use different flow rates.

Column paramet- If a named column is selected for the Column parameter in the Other:Base instruction,
er: named column the volume specified in the selected column definition will automatically be used
for column volume in the method block. The column volume for base CV cannot
then be changed in the instruction or defined as a variable. However, the Column
parameter should be defined as a variable. Choosing a column definition also
enables linear flow rate and column performance calculations.

Column paramet- If the Column parameter in the Other:Base instruction is set to Any and the Base
er: Any parameter is set to CV, the column volume is set numerically by the Volume
parameter. The column volume may be defined as a variable, allowing the scale
of the run to be decided when the method is actually run.

How to select In cases where a template or wizard-generated method and column are chosen, it
columns for a is easy to select other columns for that method on the Variables tab in Run Setup.
template or wiz-
ard Note: This might not be possible for methods that you have created yourself.

• p 155
 6 How to edit methods
6.6 How to use selected method instructions
6.6.1 Base instruction

How to select The table below describes how to select columns for a method, not selected from
columns for a a template.
method not selec-
ted from a tem-
plate Step Action
1 In the Instruction box of the Text instruction dialog box, mark the
Other:Base instruction.

2 • Select the required column from the drop-down list for the Column
parameter.
• Click the Var... button to define the Column parameter as a vari-
able. This is an optional but recommended step that will make it
easy to change the column selection for different runs.

3 • Enter a variable name and click OK.

• Click Yes to confirm.

Column definition A column definition can be chosen and defined as a variable even if the base for
the block is set to volume or time. Parameters in the column definition will then
be used for linear flow rate and column performance calculations.
Recommendation
A selected column definition applies locally within the block for which it is selected,
and is not transferred to other blocks. We strongly recommend that the column
definition be selected for the main block.

Update paramet- If you want parameters (for example, flow, pressure and averaging time) to be
ers updated when you change the column, you must define these as variables.

Pump:Methodbase Volume or column volume base is calculated from the flow rate of the SystemPump
instruction or the SamplePump, selected with the instruction Pump:Methodbase. If no
Pump:Methodbase instruction is included in the method, the default setting
SystemPump will be used.

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 How to edit methods 6

6.6.2 Instructions at the same breakpoint

Description Instructions placed at the same breakpoint in a block are executed simultaneously.
Exceptions
Exceptions are successive Block instructions, which are executed in the sequence
in which they are written. This can have important consequences in some situations.
The instruction sequence below shows an example of instructions with the same
breakpoint, where the AutoZero_UV will start after the Wash block is completed.

Breakpoint Instruction
0.00 Block WASH

0.00 AutoZero_UV

0.00 Block ELUATE

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 6 How to edit methods
6.6 How to use selected method instructions
6.6.3 Block and method length

6.6.3 Block and method length

General descrip- The time or volume of a method run is determined by the sum of the block lengths.
tion In turn, the length of a block is determined by the breakpoint of the last instruction
in the block.
Note: Depending on how conditional calls are used (see 6.7 Standard Watch
conditions on page 166), the overall method time or volume may vary according to
watch events during the run.

Block length A block in which all breakpoints are set to 0 will take no time or volume during
a method run. The illustration below shows an example of this:

To extend the length of a block without performing any other operation, set the
breakpoint of the End_block instruction appropriately, for example, as in the
illustration below:

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 How to edit methods 6

How to view the The Log Format window in the Method Editor shows the accumulated method time
accumulated or volume for the current method. The accumulated time/volume is an
method time or
volume approximation and does not take into account time or volume for Watch blocks,
Wash commands or programmed Hold. Also it does not compensate for splitter
flow.
The table below describes how to view the accumulated method time or volume:

Step Action
1 Select View:Log Format
or
click the Log Format icon.

Result: The Log Format dialog box is displayed.

2 If the method is a scouting run, click Run X to move between runs.

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 6 How to edit methods
6.6 How to use selected method instructions
6.6.4 Messages and Set_Marks

6.6.4 Messages and Set_Marks

When to use a Messages are used to inform the operator of the progress of the run. It is a good
message idea to issue messages at critical points in the method, for example, when Watch
instructions are used for conditional events.

How to add a The Message instruction can be used to set up a message that will be displayed for
Message instruc- the user during the execution of the method run. The message can be for information
tion
in a screen only, or it can require a signature before the user can control the system.
The messages are all added to the logbook text. See F.6 Appendix Messages on
page 555 for examples.
The table below describes how to add a Message instruction to the method.

Step Action
1 • Select Other in the Instructions field of the Instructions box.
• Select Message in the instructions list.

2 Type a message in the Message text box in the Parameters field.

3 Select one of the display options on the Mode menu:

• Screen, i.e. only a text message is displayed.
• Noscreen, i.e. the message will not be displayed but only inserted
into the logbook.
• Authorize, i.e. the message will require a signature from the user
before the user can interact with the system again.

4 • Select a sound on the Sound menu if desired.

• Click the Insert button.

Note: If the Message instruction is inserted in a conditional block it will only be

displayed if the conditions of the block (for example a Watch) is fulfilled.
Note: All messages are erased when the system reaches the End status. This also
includes Authorize messages.

When to use a Set_Mark instructions are useful text messages. They can be used
Set_Mark
• to insert manual notes, for example, when a problem occurs in a run
• to highlight certain stages in a method.

Set_Marks differ from Messages in that they are inserted into the chromatogram
at set points as well as into the logbook during a method run.

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 How to edit methods 6

Example of a The illustration below shows an example where Set_Marks are used to highlight
Set_Mark the start and end of fractionation in a method:

How to issue a Set_Marks are issued from the Instructions box of the Text Instructions editor. The
Set_Mark table below describes how to do this:

Step Action
1 Select Other:Set_Mark in the Instructions box.

2 Type the message in the Mark text field.

3 Click the Insert button.

Result: A new line with the Set_Mark is added to the text instruction.

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 6 How to edit methods
6.6 How to use selected method instructions
6.6.5 How to delay a method

6.6.5 How to delay a method

Introduction A method can be programmed to be delayed at critical points. There are three
instructions for this purpose: Hold, Pause and Hold_Until. These instructions are
described below.

Hold The Hold instruction suspends the execution of the method, but continues to pump
eluent at the current flow rate and concentration settings. For example, this
instruction is useful for giving the operator time to load a sample loop.
Resume the method
The method may be resumed if you click Continue on the System Control toolbar.

Pause The Pause instruction suspends execution of the method and stops the pumps so
that the system comes to a standstill. In ÄKTAdesign systems valves remain in the
position they were in before the pause. The pause may be defined as indefinite or
for a given number of minutes. This instruction is most useful for stopping the
system in the event of an unexpected condition.
Resume the method
The method may be resumed if you click Continue on the System Control toolbar.

Hold_Until The Hold_Until instruction is a special kind of Watch instruction. The method is
put on hold until a specific condition is met (signal, test or value) or the time-out
is reached. Thereafter the remaining instructions in the method are executed.
Instructions that share the same breakpoint as the Hold_Until instruction, but are
placed after it in the method, will be executed after the Hold_Until conditions have
been met.

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 How to edit methods 6

6.6.6 Linear flow rates

Introduction Linear flow rates (cm/h) can be specified for Flow instructions. The volume flow
rate is calculated from a specified linear flow rate and the column diameter as given
in the column definition.

How to use linear The table describes how to use linear flow rates.
flow rates
Step Action
1 Select a specific column on the Variables tab of the Run Setup,
or
Insert a column for the Base instruction of the block in the Text In-
structions Editor.

2 In the Instruction box of the Text Instructions editor, select Flow and
select the Linear Flow option as shown in the illustration below:

Note: If the column is changed, you will be asked if the linear flow rate or the
default flow rate should be used. If the linear flow rate cannot be used due to the
max flow rate of the system or new column, you will be advised that the max flow
rate will be used instead.

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 6 How to edit methods
6.6 How to use selected method instructions
6.6.7 Gradients and eluent concentrations

6.6.7 Gradients and eluent concentrations

Introduction Gradient instructions are given in the Text Instructions editor of the Method Editor.
This type of instruction defines gradients and immediate changes in eluent
concentration.

Parameters of the The table below shows the two parameters of the Gradient instruction:
Gradient
Parameter Description
Target Final eluent composition expressed in % eluent B.

Length Duration of the gradient.

Example of a The starting point for the Gradient is always the current eluent composition. The
Gradient instruc- instruction can be read as follows: “form a Gradient to reach Target after Length”.
tion
Example of instruction
10.00 Gradient 50{%B}, 20{base}
The example instruction above forms a gradient to 50%B (Target) starting at
breakpoint 10 with duration 20 method base units (Length). The example instruction
will finish at breakpoint 30. If the current eluent concentration is greater than
50%, the gradient will be negative.

How to form a A step gradient is an immediate change in eluent composition. To form a step
step gradient in- gradient, set the Length parameter to 0 in the Gradient instruction.
struction
Example of instruction
10.00 Gradient 50{%B}, 0{base}
The example instruction above forms a step from the current eluent composition
to 50%B at breakpoint 10. The method continues with 50%B.

Breakpoints for The breakpoint for a Gradient instruction defines the time or volume (according to
gradients method base) for the start of the gradient. A gradient with a non-zero duration
occupies time and volume in the method, and breakpoints for other instructions
may be set to occur before the gradient is completed. For most instructions, the
instruction is simply carried out at the requested breakpoint, while the gradient is
forming.

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 How to edit methods 6

Instructions that The table below describes the instructions that affect the gradient:
affect gradients
Instruction Effect
Gradient A new gradient will start at the requested breakpoint.
Any remaining duration of the previous gradient is
ignored.

Flow The eluent flow rate will change at the requested

breakpoint. If the current base is volume or column
volume, the duration of the gradient will be changed.
If the method base is time, the volume of the gradient
will be changed.

End_Method The whole method will stop, interrupting the gradient.

End_Block The gradient formation will continue uninterrupted

unless a new Gradient instruction is issued in the next
block. For example, this means that a block can be
called conditionally during gradient formation
without interrupting the gradient.

Gradients with For many purposes, it can be useful to define the length of the gradient as a variable.
variable length When this is done, breakpoints for instructions issued during or after the gradient
in the same block are automatically shifted in proportion to the length of the
gradient, with the same functionality as Change in the Text Instructions editor.

Instruction after a Any instruction that you want to insert after a gradient should be placed after the
gradient combined breakpoint and gradient length, since gradients function over time.

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 6 How to edit methods
6.7 Standard Watch conditions

6.7 Standard Watch conditions

Introduction Watch instructions allow the progress of a method run to be determined by the
events during the method run, for example, start collecting fractions when the first
peak eluates, or equilibrate the column until the eluent conductivity has reached
a given value. This is facilitated by the Watch instructions.
The system strategy includes Watch instructions for each monitor defined in the
system. These instructions are used to survey method runs, and instruct the system
to call a specified block or an instruction when a particular monitor signal meets
a given condition. As long as the condition is not met, the block is not activated.
Note: Watch instructions are shown in the Instruction box of the Text Instructions
editor, indicated in the Block pane by a green line that shows the start and duration
of the watch.

When is a Watch The breakpoint when the Watch instruction is issued determines when the watch
active? begins, not when the block is activated.
A watch is active from the point at which it is issued until
• the Watch condition is met
or
• a new watch is set for the same monitor
or
• a Watch_Off instruction is issued for the monitor.

How to insert a Watch instructions are inserted in the Instruction box of the Text Instructions Editor.
Watch instruction The table below describes how to do this.

Step Action
1 In the Breakpoint field, select the appropriate breakpoint. This decides
when the watch begins.

2 • Select Watch in the Instructions field.

• Select a Watch instruction from the list.
• Select appropriate values under Test, Value and Action in the
Parameters field.

3 Click the Insert button.

Result: The new Watch instruction is inserted on the list of actions
in the Text window.

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 How to edit methods 6

Test options in the The table below describes the Watch options that are available on the Test
Parameters field drop-down list of the Parameters field:

Option Explanation
Greater_Than The signal exceeds a certain value.

Less_Than The signal falls below a specified

value.

Slope_Greater_Than The rate of change of the signal ex-

ceeds a specified value, expressed in
monitor units/minute (for example,
mAU/min).

Slope_Less_Than The rate of change of the signal falls

below a specified value, expressed in
monitor units/minute (for example,
mAU/min).

Less_Than_Or_Valley The signal falls below a specified value

or a valley is detected. A valley is de-
tected only after a Peak_Max has been
detected, and the valley is defined by
a local minimum followed by an in-
crease to 102% of the local minimum
value plus the Delta_Peak value (see
below).

Peak_Max The signal falls to a specified fraction

of the most recent peak maximum
minus the Delta_Peak value. Factor=1
detects peak maximum.

Stable_Baseline The signal is stable within the limits

of the Delta_Base value for the period
specified by the minutes parameter.

Note: For slope values, use the Differentiate function in the Evaluation module to
measure the slope of the test chromatogram. The Simulate Peak Fractionation
technique can also be used to find the slope values.

Watch conditions Two Watch conditions are available for systems with air sensors, although they
for air sensors and may be handled differently depending on the system. The table below describes
AuxIn
the conditions and their explanations:

Condition Explanation
Equal 0 No air detected.

• p 167
 6 How to edit methods
6.7 Standard Watch conditions

Condition Explanation
Equal 1 Air detected.

Note: To use the Watch_AirSensor instruction for air sensors, the Alarm_AirSensor
setting must be disabled.

Actions when a The table below describes possible actions when a watch condition is met:
Watch condition
is met
Instruction Effect
Block name Calls the named block.

Pause, Hold Pauses or holds the method.

Continue Continues the method if paused or held.

End_block Ends the current block and return to the point from
which the block was called.

End_method Ends the method.

Ready Indicates that the next step in a MethodQueue may

start.

How to enter set- Permanent settings

tings for
Delta_Peak and Permanent settings for Delta_Peak and Delta_Base are entered with the WatchPar
Delta_Base instruction (for example WatchPar_UV, WatchPar_Cond) under System:Settings in
the System Control module (see the Administration and Technical Manual).
Temporary settings
Temporary settings that apply only for the duration of a given run can be entered
in the Instructions field of the Instruction box in the Text Instructions editor. Select
Alarms&Mon and then WatchPar.

The Delta_Peak The Delta_Peak setting helps the software to detect valleys, peaks and peak
setting maximum, and to ignore noise in the chromatogram.
The Delta_Peak value should be set
• large enough so that signal noise does not activate the conditions
and
• small enough so that the condition is activated close to the valley or peak.

As a general guideline, set the value to 2-3 times the noise level and 5-10% of the
smallest expected peak height. If you set a too high value you can prevent a new
peak from being detected after a local minimum.

03-0014-90 • p 168
 How to edit methods 6

Use of the The Delta_Peak setting

Delta_Peak setting
• sets the threshold for signal increase after a local minimum that will be
interpreted as a valley for the Less_Than_Or_Valley condition. A valley and a
new peak are detected when the signal increases to 102% of the local minimum
plus the Delta_Peak value.
Note: A valley is detected only after a Peak_Max has been detected.
Example:
If there is a local minimum at 0.05 AU and a Delta_Peak of 0.01 AU, a valley
will be detected at:
(1.02 x 0.05)+0.01=0.111 AU
• sets the threshold for signal decrease after a local maximum that will activate
the Peak_Max condition. Peak_Max is detected when the signal falls to the
specified fraction of the most recent peak maximum minus the Delta_Peak value.
The figure below illustrates the Delta_Peak setting where Peak_Max is detected
when the signal falls by Delta_Peak from a local maximum if the Peak_Max
factor is set to 1:

The Delta_Base The Delta_Base setting helps the software to determine when the baseline is
setting considered to be stable. In other words, it defines the permitted variation for the
Stable_Baseline condition. For this condition to be activated, the signal may not
vary by more than the Delta_Base value up or down over the time interval specified
in the Stable_Baseline condition in the Watch instruction.
Note: The Delta_Base setting affects the Stable_Baseline condition only.

• p 169
 6 How to edit methods
6.7 Standard Watch conditions

The condition The condition Watch Stable_Baseline is met if the signal does not deviate by more
Watch than ±Delta_Base from the baseline during the time interval specified for the watch.
Stable_Baseline
The baseline value is determined by the signal at the start of the watch. If the
condition is not met, a new interval is started with a new baseline value defined
by the signal level at the start of the new interval.
The illustration below shows an example of this:

03-0014-90 • p 170
 How to edit methods 6

6.8 How to save or delete a method template

How to save a You can save a method that you have created yourself as a template if you have
method as a tem- Edit global lists authorization (see the Administration and Technical Manual).
plate
Recommendation
The templates for each system are common for all users. Be restrictive in saving
methods as templates. We recommend that only methods that are useful for all
users be saved as templates.
The table below describes how to save a method as a template:

Step Action
1 Choose File:Save as Template in the Method Editor.
Result: The Save as Template dialog box is displayed.

2 Enter a name for the template in the Name field,

or
choose an existing template name from the Templates list that shows
the available templates within the chosen system.

3 • Select the system for which the template is intended in the For
system field.
• Select the appropriate technique on the Technique list.
• Click OK.

Result: The method is saved as a template.

How to delete a The table below describes how to delete a template:

template
Step Action
1 Choose Edit:Delete template in the Method Editor.

2 • Select the system and the template that you want to delete.
• Click the OK button and the Yes button to confirm.

• p 171
 6 How to edit methods
6.9 How to print a method

6.9 How to print a method

Instruction You can print a copy of the method, including items from the method
documentation, in Run Setup and the Text Instructions editor.
The table below describes how to print a method:

Step Action
1 In the Method Editor, select File:Print
or
click the print icon.

Result: The Print dialog box is displayed, showing the available items
from the Method Editor.

2 • Select the options you want to print.

• Click OK.

Note: For comments on the different alternatives, see "The Print

dialog box" below.

The Print dialog The table below describes some of the check box options in the Print dialog box:
box
Check box If you select this box...
Text Method all instructions will be printed, including those in
unused blocks.

03-0014-90 • p 172
 How to edit methods 6

Check box If you select this box...

Text Method:Current Ex- the method will be printed according to the current
pansion expansion in the Text pane.
(Only available from the Text Instructions editor.)

Exclude Unused Blocks only blocks that are used in the method will be prin-
ted.

Text Method: Block List only the main method and a list of the blocks that
are used in the method will be printed.

• p 173
 6 How to edit methods
6.10 How to export a method

6.10 How to export a method

Instruction You can easily export a method to another file, and save it in another format, for
instance .rtf. This is useful when you want to enable others to read the methods
without having access to UNICORN on their computers.
The table below describes how to export a method and save it to another file:

Step Action
1 In the Text Instructions editor or the Run Setup, select File:Export:Meth-
od.
Result: The Export Method dialog box is displayed.

2 Do the following:
• Select whether the current method should be exported as a
Method or as a Block list.
• Select the appropriate boxes in the Options field to define the level
of detail in the information.
• Click the Export button.

Result: The Export Method to file dialog box is displayed.

3 • Enter a file name and select the target drive and folder.
• Click the Save button.

03-0014-90 • p 174
 Scouting 7

7 Scouting

Introduction Scouting is used to repeat a series of Method runs automatically with predetermined
changes in the values for one or more Variables. A Scouting Scheme is defined as
part of the method.
This chapter describes how to set up a Scouting Scheme and define columns. The
chapter also provides some usage examples.

In this chapter This chapter contains these topics:

Topic See
How to set up a Scouting Scheme 7.1

How to define different columns for scouting 7.2

• p 175
 7 Scouting
7.1 How to set up a Scouting Scheme

7.1 How to set up a Scouting Scheme

Introduction This section describes how to set up a method for scouting.

Any parameter can be scouted, provided that it can be defined as a variable in the
method.

When to use Scouting is a facility for automatically repeating a run with systematic variation
scouting of one or more parameters. Some typical situations where scouting is useful are
when you want:
• to screen for the best column
• to find the optimal pH
• to test column capacity (sample volume)
• to find the optimal flow rate for binding and elution
• to optimize gradient length and slope
• to optimize step gradients.

Variable values The variables that appear in the scouting scheme are usually a subset of those on
the Variables tab of the Run Setup. The values in the scouting scheme can only be
set on the Scouting tab, while the default values in the method can be set either on
the Variables tab or in the Text instruction pane.
Changing variable values in the scouting scheme does not change the values on the
Variables tab or in the text instructions. Values for variables selected for scouting
are grey on the Variables tab and cannot be changed there.
Any changes that you make to variable values when a scouting scheme is run are
saved in the result file. Results from a scouting run are saved in a scouting folder.

Scouting tab but- There are seven buttons on the Scouting tab of the Run Setup plus the Help button.
tons The table describes the functions of these buttons:

Click the button... if you want...

Define... to define new scouting variables. The Scouting Vari-
ables dialog box is displayed, and you can select
variables to be used in the scouting series.
Note: The variables that have been selected for
scouting cannot be changed on the Variables tab.

Clear All to clear all runs.

This converts the scouting run to a non-scouting run
so that it contains only the original method and val-
ues.

03-0014-90 • p 176
 Scouting 7

Click the button... if you want...

Delete to remove a run from the Scouting tab.
Click on any variable in the run you want to remove,
and then click the Delete button.

Insert to insert a new scouting run before an existing run.

Click on a run column and then click the Insert but-
ton. The new run will inherit the variable values from
• the preceding run,

or
• from the default values in the method if the run is
inserted at the beginning of the scouting series

Add • to add a scouting run if there are no runs previ-

ously in the scheme. Default values will be used
for the first run.

or
• to add a scouting run after all other runs in the
series. The new run inherits the values from the
run that precedes the new run.

Series... to set up a series of runs with differing inputs.

Edit Variable... to rename or delete a variable, or change a variable

into a detail variable.

How to set up or The table below describes how to set up or edit a scouting scheme.
edit a Scouting
Scheme
Step Action
1 Create a method. If you do not use a template or wizard, define ap-
propriate variables in the method.

2 In the Run Setup, click the Scouting tab.

Result: If no scouting variables have been previously defined, the
Scouting Variables dialog box is displayed. If not, click the Define
button.

• p 177
 7 Scouting
7.1 How to set up a Scouting Scheme

Step Action
3 Select the variables you want to scout. If you cannot find the variable
you want, use the following options:
• Show details to display variables created with the Visible in details
only option.
• Show unused variables to display all variables, including those
that are not used in the method.

Click OK.
Result: The selected scouting variables will appear in a column, with
default valued inserted.

4 Make any required changes in the scouting variable values.

5 To add a new Run column, click the Add button to copy the values
from the last run column, and then change variable values as re-
quired.

6 Repeat steps 3 to 5 as required until you have defined all the scouting
runs you need.

7 To exclude scouting runs from the default scouting scheme, right-

click the heading of the run. To include the scouting run again, right-
click it again.

8 Click the Start Protocol tab in Run Setup.

Select from the following options:
• The Scouting box: Select this to display the Scouting page at the
start of a run. This allows the operator to adjust the values for
scouting variables before the method run starts.
• The First run only button: Select this to display the start protocol
before the first run only. The settings entered in the Start Protocol
for the first run will apply throughout the run, and the scouting
series will be performed automatically without user intervention.
• The All runs button: Select this to display the Start Protocol before
each run in the scheme. This gives the operator an opportunity
to change variable values or fill the sample loop before each run.

Note: The operator must then click the Start button before each run.

03-0014-90 • p 178
 Scouting 7

How to set up The table below describes how to set up series.

series
Step Action
1 Select a cell on the Scouting tab, and click the Series button.
Result: The Insert Series dialog box is displayed.

2 In the Insert Series dialog box, type the selected series values (within
the specified range limits), separated by commas, and click OK.
Result: A new set of runs is inserted on the Scouting tab with the
values provided.

How to delete or Scouting variables can be deleted or renamed in the scouting scheme in the same
rename scouting way as in the Variables tab. The table below describes how to delete or rename a
variables
variable in the Scouting tab.

Step Action
1 Click the Edit Variable... button on the Scouting tab.
Result: The Edit Variables dialog box opens. The variables are listed
alphabetically.

2 Select the variable to edit.

3 Rename
• Type a new variable name in the New name text box.
• Click the Rename button.

Result: The variable is renamed.

Delete
• Click the Delete button.
• Confirm that you want to delete the variable.

Result: The variable is deleted.

How to change a Detail variables are indicated with a D to the left of the Variable column on the
scouting variable Scouting tab. The table below describes how to set up a detail variable.
into a detail vari-
able
Step Action
1 Click the Edit Variable... button on the Scouting tab.
Result: The Edit Variables dialog box opens. The variables are listed
alphabetically.

2 Choose the variable to be changed.

• p 179
 7 Scouting
7.1 How to set up a Scouting Scheme

Step Action
3 • Select the Set visible in details only checkbox.
• Click the Close button.

Result: The variable is indicated by a D.

Note: De-select the checkbox to make the variable fully visible again.

How to copy con- The contents of the Scouting tab can be copied and pasted into a third-party factorial
tents to factorial design program. Processed values can then be pasted back into the Scouting tab.
design programs
The table below describes how to do this:

Step Action
1 Select the text, etc., that you want to copy.

2 Press Ctrl+C.

3 Place the cursor where you want to insert the copied text.

4 Press Ctrl+V.

03-0014-90 • p 180
 Scouting 7

7.2 How to define different columns for scouting

Instruction You can define different columns for use in the various scouting runs. However,
in selecting a different column, other variables may also be changed between runs.
The table describes how to scout columns.

Step Action
1 Choose a method with a column (not Any). Alternatively, you can
have a method with CV as the main base and a column (not Any)
selected as a variable called “column”.

2 Click the Define button on the Scouting tab.

Result: The Scouting Variables dialog box is displayed.

3 Select Column and click OK.

4 Click the Column drop-down menu item within the desired run.
Result: A menu is displayed.

5 Select a column.
Result: The Column Value Update dialog box is displayed.

6 The dialog box asks you whether you want to update the instructions
with column default values. Select one of the following:
Yes
The method for the scouting run is updated with variable parameter
values for the selected column, consisting of UV average time, pres-
sure limit, flow rate, etc. These parameter values are added to the
scouting variables on the Scouting tab. Note that the updated para-
meter values may differ from the values for the same variables in
other scouting runs.
No
No changes are made. The method retains the parameter values
corresponding to the column that were either originally selected
during creation of the method, or included in an earlier version of
the method on the Scouting tab for which the default values for that
column were accepted.
Note: If the method contains a linear flow rate instruction, the user
can keep the linear flow rate by selecting a check box in the dialog
box.

• p 181
 8 MethodQueues

8 MethodQueues

Introduction MethodQueues provide a means for linking several methods together, on the same
or different systems. For example, if a system wash procedure is programmed in
a separate method, it can be linked in a MethodQueue to a series of different process
methods, ensuring that the same wash procedure is used before every process.
Alternatively, the product of a separation on one system might form the starting
material for a separation on the next, allowing fully automated multi-step
processing.

In this chapter This chapter contains these topics:

Topic See
How to create a new MethodQueue 8.1

How to edit a MethodQueue 8.2

03-0014-90 • p 182
 MethodQueues 8

8.1 How to create a new MethodQueue

Instruction The table below describes how to create a MethodQueue in the UNICORN Manager
module.

Step Action
1 • Select File:New:MethodQueue.

or
• Right-click in the Methods window and select New:MethodQueue
on the shortcut menu.

or
• Click the MethodQueue icon.

Result: The MethodQueue Editor dialog box is displayed.

2 The default selection for Start MethodQueue is As soon as possible.

• Click the At time radio button and select a time and weekday for
the start of the MethodQueue, if desired.

3 • Double-click the cell in the first row of the System column.

Result: The Method for row number 1 System dialog box opens.
Note: See "How to set up MethodQueues on several systems" below
if you have more than one system available.

4 • Select a method and click OK.

Result: The method is displayed in the System column.

• p 183
 8 MethodQueues
8.1 How to create a new MethodQueue

Step Action
5 • Click the Insert Row After button and repeat steps 3 and 4 to add
more methods to the MethodQueue.

Note: The timing of MethodQueue steps performed on different sys-

tems can also be controlled by the Ready instruction in the method
(see "Relative timing of steps" below).
By default, each method step will start as soon as possible (ASAP)
after the completion of the previous method step. Use the Condition
cell of the chosen method to set another time interval for starting a
selected step.
• In the Conditions column, double-click the cell for the method to
be delayed.

Result: The Condition for row number X dialog box opens.

Note: Use the Previous Row and Next Row buttons to select other
methods for editing.
• Click the Wait radio button, select the number of hours and
minutes that the method is to be delayed and click OK.

Result: The execution of the MethodQueue will be held for the selected
number of hours and minutes and then resume.
• Click the Save button to save the method.

Result: The Save MethodQueue dialog box opens.

• Type a file name and click the Save button.

How to set up If you have more than one system available, the System column will not be displayed
MethodQueues on at first in the MethodQueue Editor. The table below describes how to set up a
several systems
MethodQueue for several systems.

Step Action
1 • Click the MethodQueue icon.
• Click the Add System button and select a system for the first
MethodQueue step from the Add System dialog box.

03-0014-90 • p 184
 MethodQueues 8

Step Action
2 • Repeat this for each system when you want to use a different
system in the MethodQueue.

Result: Another system column will be added for each additional

system.

Relative timing of The setting of the Condition dialog box (reached by double-clicking a Condition cell
steps in the MethodQueue Editor dialog box), determines the relative timing of the steps
of a MethodQueue. If successive methods are run on the same system, the timing
set in Condition applies from the completion of one method to the start of the next.
If successive methods are run on different systems, you can use the Ready instruction
in one method to trigger the start of the next method. In this way, you will be able
to start the next method before the current method has ended. The Condition setting
then applies from the Ready instruction to the start of the triggered method. This
is useful in situations where a method on one system prepares the starting material
for the next, and then continues to wash the system. See the example below:

Instruction to System 1 Instruction to System 2

Apply sample

Eluate

READY Apply sample

Wash Eluate

Unattended opera- The Start Protocol for each method step in the MethodQueue is displayed when the
tion of the Meth- corresponding method is run. If you want the MethodQueue to operate unattended
odQueue
you must ensure that the methods do not include a Start Protocol.
See 5 How to create a method on page 79 for more information.

• p 185
 8 MethodQueues
8.1 How to create a new MethodQueue

How to hold a The table below describes how you can create a MethodQueue if you try to start a
method in queue new method run while the system is still busy with another method run.
while the system is
busy
Step Action
1 Right-click on the method in the UNICORN Manager module and select
Run:system name on the shortcut menu.
Result: The System Busy dialog box opens.

2 • Select the Add the method to a MethodQueue that will execute as

soon as the system is free option.
• Click OK.

Result: A MethodQueue will automatically be created in the default

queue folder. The name of the MethodQueue will be the same as the
method name, followed by a five-digit sequence number.

3 The method will be executed as soon as the system is free.

Note: A warning note is displayed in the System Busy dialog box if
the method includes a Start Protocol. The Start Protocol must be
completed at the start of the method run before it can be executed.

03-0014-90 • p 186
 MethodQueues 8

8.2 How to edit a MethodQueue

Method Queues MethodQueues are saved in a separate folder within the folder that you specified
are saved in a sep- when you saved the MethodQueue. The MethodQueue folder is represented by a
arate folder
special icon in the Methods window of the UNICORN Manager.

A MethodQueue folder contains the MethodQueue definition and copies of all included
methods.

How to edit a The MethodQueue files are copies of the original method files. If changes are made
MethodQueue file in the original method, these will not affect the method in the MethodQueue.

To avoid confusion between different versions of method files, make sure that
MethodQueue definitions always contain updated methods. To implement changes
in a MethodQueue method, do one of the following:
• Edit the method in the MethodQueue folder,
or
• Edit the original method, then use the MethodQueue editor to update the
MethodQueue, and replace the old method with the changed version.

Instruction The table below describes how to edit an existing MethodQueue.

Step Action
1 Right-click the selected MethodQueue folder icon in the UNICORN
Manager, and select Edit from the displayed menu.
Result: The MethodQueue Editor dialog box is displayed.

• p 187
 8 MethodQueues
8.2 How to edit a MethodQueue

Step Action
2 Select a table row to edit and do the following as required:
• Double-click the System cell and select a new method from the
Method for row... dialog box.
• Double-click the Condition cell and edit the delay time for the
method.
• Click the Add System button to add a new system to the queue
and use it for a MethodQueue step.
• Click the Delete System button to remove a system and all associ-
ated methods from the MethodQueue.
• Click the Insert Row Before or Insert Row After buttons to add new
rows before or after the selected row.
• Click the Delete Row button to remove the selected row.
• Click the Move Row Up or Move Row Down to move the selected
row one step up or down in the queue.

3 • Click the Save button.

• Click the Run button to execute the MethodQueue immediately or
the Close button to close the dialog box.

03-0014-90 • p 188
 How to perform method runs 9

9 How to perform method runs

Introduction This chapter describes how to perform and monitor different kinds of method runs
from the System Control module. It also describes how to control the system with
manual commands and instructions.

In this chapter The chapter contains these sections:

Topic See
How to start a method run 9.1

How to monitor a method run 9.2

Manual system control 9.3

How to perform a scouting run 9.4

How to perform a MethodQueue run 9.5

If the network connection fails 9.6

• p 189
 9 How to perform method runs
9.1 How to start a method run

9.1 How to start a method run

Before you start Before you start a method, make sure that
• the correct system is connected in control mode

Note: If the system is connected via a CU-950 Advanced unit, the Ethernet connection
must not be broken during the start-up phase of the method run.

How to start from You can start a method from the UNICORN Manager in two ways:
the UNICORN
Manager • Select a method in the Methods window and select File:Run.
• Select a method, right-click and select Run from the displayed menu.

How to start from The table below describes how to start a method run from System Control:
System Control
Step Action
1 Select File:Run
or
click the Run button.
Result: The Run dialog box is displayed.
Note: The Run button will open the method that was used for the
previous run, if a run has been performed since you logged on.

2 Select a method and double-click the method icon.

Result: The method run starts. If the method includes a Start Protocol
this must be completed before the actual method run starts. Se further
instructions below.

How to add meth- For methods that are used frequently (for example column cleaning methods or
ods to the File routine separations), it may be convenient to define the methods as commands in
menu
the File menu.
The table below describes how to define a method as a command:

Step Action
1 Choose File:Menu in System Control and select the required method.

2 Click the Add button and click OK.

Result: The method name will appear as a command in the File
menu. If you choose the command, the method will start.

03-0014-90 • p 190
 How to perform method runs 9

How to start an You can start a method template or wizard directly if your system has defined
instant run templates or wizards.
To do this, either
• click the Instant Run icon in the UNICORN Manager toolbar

or
• select File:Instant Run in System Control.

How to use the If the method is defined with a Start Protocol, this will be displayed before the
Start Protocol method actually starts.
The table below describes how to use the Start Protocol:

Step Action
1 • Start the method run.

• Work through the start protocol, answering questions as required.

The start protocol items that can be displayed are described in 6.5.14
The start protocol tab on page 151.
• As each screen is completed, click the Next button to move to the
next screen or the Back button to return to the previous screen.

2 Click the Start button in the last window to start the run.

Confirm/Sign authorization for the Start Protocol

If there are any questions in the Start Protocol that require authorized confirmation,
you will be asked for a user name and password when you attempt to leave the
screen containing the questions. Only users with Confirm/Sign authorization may
authorize answers to such questions. Each question that requires an authorization
must have a separate authorization.

How to start a If the system is busy with a method run in progress, you can still start a new method.
method when the You will have the option to place the method in a MethodQueue, which can be
system is busy
executed as soon as the system becomes available again. The table below describes
how to do this.

Step Action
1 • While a method run is in progress, right-click on the next method
you want to run and select Run:System.

Result: The System Busy dialog box opens.

• p 191
 9 How to perform method runs
9.1 How to start a method run

Step Action
2 • Select the Add the method to a MethodQueue that will execute as
soon as the system is free option.
• Click OK.

Result: A MethodQueue will automatically be created in the default

queue folder. The name of the MethodQueue will be the same as the
method name, followed by a five-digit sequence number.

3 The method will be executed as soon as the system is free.

Note: A warning note is displayed in the System Busy dialog box if
the method includes a Start Protocol. The Start Protocol must be
completed at the start of the method run before it can be executed.

Note: See 8.2 How to edit a MethodQueue on page 187 for more information.

03-0014-90 • p 192
 How to perform method runs 9

9.2 How to monitor a method run

Introduction This section describes how to monitor a method run by using the System Control
module and how to customize the different panes.

In this section The table shows the topics that can be found in this section.

Topic See
How to customize System Control panes 9.2.1

The Run Data pane 9.2.2

The Curves pane 9.2.3

The Flow Scheme pane 9.2.4

The Logbook pane 9.2.5

• p 193
 9 How to perform method runs
9.2 How to monitor a method run
9.2.1 How to customize System Control panes

9.2.1 How to customize System Control panes

Introduction The System Control module displays the status of the current system. On the
Windows taskbar, there may be up to four System Control modules available that
can be connected to different systems. Separate systems may be controlled and
displayed independently of each other.

Illustration The illustration shows the System Control module with the Run Data, Curves, Flow
scheme and Logbook panes displayed.

How to select Each System Control module displays up to four panes for monitoring different
what panes to dis- aspects of the run. To select what panes to display, either
play
• click the Customize Panes icon,

or
• choose View:Panes.

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 How to perform method runs 9

How to customize Change the size

System Control
panes Select a split-bar and drag up and down to change the size of a specific pane.
Maximize, restore or hide
Right-click a pane and select the appropriate option to:
• maximize,
• restore
or
• hide the pane.

• p 195
 9 How to perform method runs
9.2 How to monitor a method run
9.2.2 The Run Data pane

9.2.2 The Run Data pane

Description The Run Data pane displays the current values for selected run parameters. The
update interval is defined in the system strategy.
The figure below displays an example of the Run Data pane:

How to change The appearance of the pane can be changed so that it includes more or fewer data
the appearance of displays. The table below describes how this is done:
the pane

Step Action
1 In System Control, select View:Properties
or
right-click on the pane and select Properties on the menu.
Result: The Properties dialog box is displayed.

2 Select the Run Data Groups tab and, if desirable, do one or more of
the following:
• Select an available group to be displayed in the list to the left.
• Edit an available group: Select the group from the list on the left,
and click the Edit Group button. Modify the included readings in
the list to the right, and click OK.
• Create a new group: Click the New group button and select the
readings that you want to view from the list. Enter a name for
the group, and click OK.
• Delete a group: Click the Delete Group button and select a group
in the Delete Layout dialog box, click OK and confirm the deletion.

3 Select the run data parameters that you want to display in the list
to the right.

4 Click OK to view the selected items in the Run Data pane. The name
of the selected layout replaces the default layout name Run Data.

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 How to perform method runs 9

How to change The table describes how to change the text color or background in the displayed
text color or text reading boxes.
background

Step Action
1 Right-click on the pane and select Properties.
Result: The Properties dialog box is displayed.

2 Select the Run Data Color tab.

3 Click the Text or Background buttons.

Select a new color, and click OK.
Result: The color change is displayed in the test field.

4 Make further adjustments to the colors as appropriate.

5 Click OK to apply the changes.

How to set the If the Pressure reading box is displayed in the Run Data pane, you can set the
pressure units displayed units. The table below describes how this is done:

Step Action
1 Right-click on the Pressure reading box to display the menu.

2 Select Set Unit and the appropriate unit (MPa, bar or psi).
Result: The selected unit is displayed.

How to view and Some strategies directly link specific manual instructions to the reading boxes in
select manual in- the Run Data pane. This is indicated by a double arrow (>>). A particular reading
structions
box can have one or more instructions attached to it. In cases where there is more
than one instruction, one of the instructions is the main instruction.
There are two ways to view the manual instructions:
Option 1:
• Double-click the reading box.
Result: The dialog box for manual instructions is displayed, showing the
instruction, or main instruction if there is more than one.

Option 2:
• Right-click the reading box. Select Instructions in the displayed menu. Another
menu shows the specific manual instruction(s).
• Click an instruction to select it.

Result: The dialog box for manual instructions is displayed in which you can
execute the appropriate command.

• p 197
 9 How to perform method runs
9.2 How to monitor a method run
9.2.2 The Run Data pane

For more details on how to use manual instructions, please see 9.3.2 Manual
instructions on page 212.

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 How to perform method runs 9

9.2.3 The Curves pane

Introduction The Curves pane of the System Control module displays monitor signal values
graphically.
The figure below shows an example of the Curves pane:

How to select You can decide which curves you want to display in the Curves pane. Curves will
curves to be dis- only be shown for components present in the chromatography system.
played
The table describes how to select the curves to be displayed on the screen.

Step Action
1 In System Control, select View:Properties.
Result: The Properties dialog box is displayed.

2 Select the Curves tab.

Note: The curves in the list are those for which Store is set to On in
the system settings, together with any reference curves defined in
the method.

3 In the Display curves list, select the curves you want to display.
If you want all curves to be displayed, click the Select All button. If
you do not want any curves to be displayed, click the Clear All button.
Click OK.

How to display a The table below describes how to display a vertical marker line:
vertical marker
line
Step Action
1 Right-click the Curves pane and select Marker.

2 Drag the marker line with the mouse.

Result: Where the line bisects the curve, the X-axis and Y-axis values
are displayed at the top right corner of the pane.

• p 199
 9 How to perform method runs
9.2 How to monitor a method run
9.2.3 The Curves pane

Note: Right-click and select Snapshot to record the marker position values. See
2.2.7 Snapshots on page 41 for more information about the Snapshot function.

How to set a refer- When the vertical marker is displayed, you can set a reference point to display
ence point curve data. The table describes how to set a reference point:

Step Action
1 • Display a Marker in the Curves pane.
• Right-click and select Set Marker Ref. Point to define a reference
point for the marker position.

2 When the marker is moved from the reference point, the X-axis and
Y-axis values for the new position are displayed together with:
• the new position in relation to the position of the reference point,
• the minimum, maximum and average values for the curve interval
between the reference point and the new position.

How to change The Curves pane displays graphs for the selected curves in different colors, with
the curve colors any reference curves included with the method as dashed lines.
and styles
The table below describes how to change the curve colors and styles:

Step Action
1 Select View:Properties.
Result: The Properties dialog box is displayed.

2 Select the Curve Style and Color tab.

3 • Select a curve from the Curve list.

• Select an appropriate color and style.

How to change In most cases, the Y-axis is automatically scaled for each of the curves. Values on
the scale of the Y- the Y-axis apply to the curve with the same color as the axis markings. To get the
axis
correct Y-axis, click the legend. The table below describes how to fix the scale of
individual curves.

Step Action
1 • Select View:Properties.

Result: The Properties dialog box is displayed.

• Select the Y-axis tab.

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 How to perform method runs 9

Step Action
2 • Select the appropriate curve.
• Select Fixed and type a minimum and maximum range in the fields
within the specified limits.

3 Repeat step 2 for other curves if needed.

4 Click OK.

How to change The table below describes how to change the scale of the X-axis:
the scale of the X-
axis
Step Action
1 • Select View:Properties.

Result: The Properties dialog box is displayed.

• Select the X-axis tab.

2 Select the appropriate base, Time or Volume.

Note: Curves are collected in time and recalculated for display in
volume. Thus, the resolution of the two bases may appear slightly
different.

3 Select the appropriate Axis scale:

• Total will show the curves as far as they have come in the run.
• Window allows you to set the portion of the total pane to be dis-
played, either in minutes or ml depending on the selected base.
• Adjust retention zero to injection sets the retention value to zero
at the point of the first injection.
• Click OK.

How to switch • Click the legend of the X-axis

between time and
volume units or
• right-click and select Base Type

to switch the display between time and volume units. The run is controlled according
to the time/volume base defined in the current block, regardless of the base in the
curves display.

• p 201
 9 How to perform method runs
9.2 How to monitor a method run
9.2.3 The Curves pane

How to zoom in The table below describes how to zoom in on a selected region of the curve pane:
the Curves pane
Step Action
1 • Press and hold the left mouse button and drag a rectangle out on
the screen to encompass the area to be viewed.
• Release the mouse button.

Result: The display is now zoomed in on the selected area.

2 Repeat the process for further magnification of selected areas.

How to zoom out

To reduce the scale of the zoom, right-click in the Curves pane, and select one of
the following options:
• Undo Zoom: reverses each zoom-in action a step at a time.
• Reset Zoom: reverses all zoom-in actions to the default scale.

How to select If the Pressure curve is displayed in the Curves pane, you can set the displayed units.
curve pressure The table below describes how to do this:
units

Step Action
1 Right-click in the Curves pane, and select Properties in the displayed
menu.
Result: The Properties dialog box is displayed.

2 Select the Y-Axis tab.

3 Select the Pressure curve and select the appropriate Pressure unit
button.
Click OK.

How to edit text You can select the way that text is aligned for the Logbook and Fraction curves.
in the Curves pane You can also select to show only part of the Logbook information. The table below
describes how to do this:

Step Action
1 Right-click in the Curves pane, and select Properties in the displayed
menu.
Result: The Properties dialog box is displayed.

2 Select the Curve Style and Color tab.

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 How to perform method runs 9

Step Action
3 Select the following:
• Logbook or Fraction curve in the Curve list as appropriate.
• Select the appropriate Logbook text alignment or Fraction text
alignment option:
- Horizontal
- Vertical
- Fly over (displays the text if you place the mouse pointer over
the generated mark).

4 To filter the type of Logbook information overlaid on the Curves

pane, do the following:
• Click the Filter button.
Result: The Filter Logbook dialog box is displayed.
• Select the appropriate check boxes and set the maximum block
depth.
• Click OK to return to the Curve style and Color tab.

5 Click OK.

How to view the At some breakpoints there can be more logbook information than what is possible
complete logbook to conveniently display in the Curves pane. The additional information that is not
information
displayed is indicated by an arrow point symbol by the break point.
• Hold the mouse cursor over the break point to display the complete information
in a flyover text box, as shown in the illustration below.

• p 203
 9 How to perform method runs
9.2 How to monitor a method run
9.2.4 The Flow Scheme pane

9.2.4 The Flow Scheme pane

Introduction The flow scheme is a graphical representation of the chromatography system that
shows the current status of the run. During a run, the flow scheme displays open
flow path(s) in color and monitor signals with numerical displays.

Illustration The illustration below shows an example of a flow scheme for a run:

How to stretch a The flow scheme can be stretched to fit the screen. To do this, right-click in the
flow scheme pane and select Stretch in the shortcut menu.

How to view and Some strategies link specific manual instructions directly to the components in the
select flow scheme flow scheme pane. The components in the flow scheme that are associated with
manual instruc-
tions instructions are indicated with double arrows (>>). A particular component can
have one or more instructions attached to it. In cases where there is more than one
instruction, one of the instructions is the main instruction.
To display and select instructions:
• double-click a component

or
• right-click a component, select Instructions and an instruction in the shortcut
menu.

Result: The manual instructions dialog box for the selected instruction type opens.

03-0014-90 • p 204
 How to perform method runs 9

9.2.5 The Logbook pane

Introduction All actions (including method start and end, base instruction, method instructions
and manual interventions such as Pause or Hold) and unexpected conditions such
as warnings and alarms are logged for every run, with date, time and current user
name where appropriate. The logbook thus provides a complete history of any
given run. The log is saved in the result file.

Illustration The illustration below shows an example of the Logbook pane:

Note: The second logbook line is the BatchID that is automatically generated.

Autoscroll The Logbook pane can autoscroll to display the latest entries. Right-click in the
pane, and select Autoscroll. You can also select the Autoscroll option in the Properties
dialog box (View: Properties and select the Logbook tab).

How to filter the You can choose to display only selected items in the logbook. The table below
logbook contents describes how to activate the filter.

Step Action
1 • Right-click in the Logbook pane and choose Properties.

Result: The Properties dialog box opens.

2 • Choose the Logbook tab.

• Select the items you want to display in the logbook (all items are
selected by default).
• Click the OK button.

Result: Only the selected items will be displayed in the logbook. The
Logbook title in the upper right corner will show the text (Filter on)
to indicate that not all items are visible. All items will still be logged
in the result file.

• p 205
 9 How to perform method runs
9.2 How to monitor a method run
9.2.5 The Logbook pane

How to find log- The logbook can be searched for specific text entries. The table below describes
book text entries the function:

Step Action
1 Right-click in the Logbook pane and choose Find.
Result: The Find dialog box opens.

2 • Type the text you want to locate.

• Select search criteria if necessary.
• Click OK.

Result: The located logbook entry is highlighted.

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 How to perform method runs 9

9.3 Manual system control

Introduction This section describes how to control the system with manual commands and
instructions.

In this section This section contains these topics:

Topic See
The toolbar and status bar 9.3.1

Manual instructions 9.3.2

Alarms and warnings 9.3.3

• p 207
 9 How to perform method runs
9.3 Manual system control
9.3.1 The toolbar and status bar

9.3.1 The toolbar and status bar

Toolbar buttons The toolbar at the top of the System Control module contains three sets of buttons:
• Manual Direct Commands buttons for starting and stopping the run
• Windows buttons to access dialog boxes for pane selection, documentation and
layout properties
• System Access buttons to control the system connection.

Show and hide

The toolbars can be shown and hidden by choosing View:Toolbars and selecting
the relevant boxes.
The figure below shows the toolbar:

Manual Direct The available Manual Direct commands buttons in System Control are dependent on
Commands the control status of the connection. The table below shows when each button is
available:

Control Status Available buttons

End Run

Running Hold, Pause, End

Manual Run, Pause, End

Hold Pause, Continue, End

Method pause Hold, Continue, End

Manual pause Run, Continue, End

Direct command The table below describes the functions of the Manual direct command buttons.
button functions
Button Function
Run Opens the Run dialog box, which shows all available
methods, as the first step in a method run. If a method
is loaded, Run Setup opens. The run will start imme-
diately if a start protocol isn't part of the method.

03-0014-90 • p 208
 How to perform method runs 9

Button Function
Hold Suspends execution of a method, but continues
• to pump liquid at the current flow rate and eluent
concentration settings. All settings remain un-
changed.
• to increase accumulated time and volume.

Method instructions are not executed until the Contin-

ue button is pressed.

Pause Behavior of the Pause button is strategy-dependent.

The Pause button suspends execution of a method
and stops all pumps so that the system comes to a
standstill.
For ÄKTAdesign systems, valves remain in the posi-
tion they were in before the pause.
Accumulated time and volume is not increased during
a Pause.
Method instructions are not executed until Continue
is pressed.

Continue Resumes execution of a paused or held method.

End • Terminates method execution

• Puts the system into an End state.

Note: You can choose to save the partial result or

discard it.

Note: The commands can also be found on the Manual menu.

Windows buttons The table below describes the functions of the Windows buttons:

Button Function
Opens a dialog box where you can choose which
window panes to display. This button is equivalent
to the menu command View:Panes.

Opens the documentation pages. Run notes can be

entered on the Notes tab and settings can be changed.
Other tabs are displayed for information only. This
button is equivalent to the menu command
View:Documentation.

• p 209
 9 How to perform method runs
9.3 Manual system control
9.3.1 The toolbar and status bar

Button Function
Opens the properties pages. This button is equivalent
to the menu command View:Properties.

System Access There are two functions of the System Access buttons:
buttons
Disconnect/Connect system

The Disconnect button is used to disconnect the system and leave it in a locked or
unlocked state.

The Connect button connects the system.

Leave/Take control of the system

The Leave control button leaves the system in a locked or unlocked state.

The Take control button takes control of the system.

Status bar, connec- The status bar displays a message indicating the connection status of the window.
tion status The table below describes the different messages:

Message Connection status

Controlled by: <user> The indicated user has a control mode connection to
the system. Other users can establish a view mode
connection.

03-0014-90 • p 210
 How to perform method runs 9

Message Connection status

Locked by:<user> The indicated user has left the system in a locked
state. Users who can supply the required password
can unlock the system and establish a connection.
The password is case sensitive.
Note: It is possible to unlock with the "lock" pass-
word or with the UNICORN logon password. Any-
one who uses the UNICORN logon password must
have Unlock systems access rights. The "lock" pass-
word is the password entered by the user who locked
the system.

System is available Any user can establish a connection.

Status bar, Watch The status bar displays a message indicating if a Watch is active in the method.
status
• Click the Active watch status message to open the Watch dialog box with
information about the active Watch instruction.

• p 211
 9 How to perform method runs
9.3 Manual system control
9.3.2 Manual instructions

9.3.2 Manual instructions

Introduction The chromatography system can be controlled with manual instructions issued
from the Manual menu in the System Control module. The available instruction
options are dependent on the strategy.

Manual instruc- Manual instructions can be issued while a method is running. A manual setting
tions during a applies until the next method instruction of the same type is executed
method run
Example: A manual Flow instruction will set the flow rate until the next Flow
instruction in the method is executed. Manual instructions that you issue during
a method are recorded in the logbook for the method run.

The manual in- The Manual menu in System Control opens a dialog box similar to the Instruction
structions dialog box in the Method Editor. The name of the connected system is displayed on the title
box
bar of the dialog box. See an example in the illustration below:

Note: The parameter values will be updated continually during the run if the Auto
update checkbox is selected.

Column protec- Your user attributes may include a requirement to always set pressure alarms.
tion
Step Action
1 When you try to execute a pump instruction the Column protect mode
dialog box opens.

2 • Click the Yes button in the dialog box to select a column and re-
trieve the correct maximum pressure value.
• Click OK to close the column list.
• Click the Insert button to add the Alarm_Pressure instruction.

3 • If necessary, repeat step 2 to add an Alarm_SamplePressure instruc-

tion.

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 How to perform method runs 9

How to use manu- Manual instructions are entered in the same way as method instructions from the
al instructions dialog box in the Method Editor. The table below describes how to add a manual
instruction:

Step Action
1 • Select an instruction group and a component in the Instructions
field.
• Select instruction parameters in the Parameters field.

2 • Click the Insert or Execute buttons as needed. (See the descriptions

of the different functions below)

The buttons of the The table below describes the functions of the manual instructions buttons:
manual instruc-
tions dialog box
Button Function
Insert This button places the current instruction in the list
at the bottom left of the dialog box.

Delete This button deletes the selected instruction from the

current list only. One instruction can be deleted at a
time.

Execute This button

• executes all instructions in the list at the same time

or
• executes the currently marked instruction if the
list is empty.

Note: Although all instructions are executed simultan-

eously, some (for example gradient and fraction in-
structions) may take some time to complete in the li-
quid handling module.

Close If you click the Close button without first clicking the
Execute button, commands in the list
• will not be executed

• will be deleted from the command list.

• p 213
 9 How to perform method runs
9.3 Manual system control
9.3.2 Manual instructions

How to save When you choose to run the system manually - as opposed to a Method run - the
manual results results are automatically stored in a folder called Manual Runs. The Manual Runs
folder stores the ten most recent results from your manual runs. To save a result
file from the Manual Runs folder more permanently, you need to move or copy it
to another location.
An alternative way to save the results from a manual run is to record the results
manually in a result file. The table below shows how to do this:

Step Action
1 • Choose Manual:Other.
• Select the instruction Record On at the beginning of the run.

2 • Click the Execute button.

Result: UNICORN will prompt for a result file name.

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 How to perform method runs 9

9.3.3 Alarms and warnings

Introduction Alarms and warnings are displayed regardless of the activity currently in progress
in UNICORN. You will be notified of an exceeded limit in a running system even
if you are developing a method, evaluating data or monitoring a method run on a
different system. Warnings and alarms are also recorded in the logbook for the
run.

Limits for monitor The system settings determine the acceptable limits of monitor signals during a
signals run. The limits can also be set for the current run by an instruction in the method.
Limits set with a method instruction override the limits set in system settings. If
these limits are exceeded in a run, a warning or alarm dialog box is displayed on
the screen.

Effects of alarms Alarms and warnings have different effects on the system:
and warnings
• Warning: The run continues.
• Alarm: The system is paused.

In a network sys- In a network installation, alarms and warnings are displayed on the controlling
tem station and all stations viewing the system. An alarm can be acknowledged only
from the computer connected in control mode. Alarms are displayed but cannot
be acknowledged on computers connected in view mode.

• p 215
 9 How to perform method runs
9.4 How to perform a scouting run

9.4 How to perform a scouting run

More information See 7.1 How to set up a scouting scheme on page 176 for information on how to
on scouting runs set up scouting runs.

Instruction The table below describes how to perform a scouting run:

Step Action
1 Start the method (see 9.1 How to start a method run on page 190).
Result: The Start Protocol will display the scouting scheme as defined
in the method (assuming that the Scouting box is selected on the
Start Protocol tab of the Run Setup).

2 Check through the settings for the scouting scheme in the Scouting
tab, and if required, do the following:
• Change the scouting variable values.
• Right-click the top of the Run column to toggle the run status
between Run and Excluded.

3 Work through the rest of the Start Protocol.

Click the Start button.

Results of a scout- The results of a scouting run are saved in a special scouting folder as defined in
ing run the Results tab of the Start Protocol. Within the folder, each run is saved in a separate
result file named according to the usual naming rules (see 6.5.12 The result name
tab on page 147).
If the Start Protocol is displayed for each run in a scouting scheme, you are able to
change the result file name during scheme execution.

How to change • At any time during a run, you can click the View Documentation icon in System
scouting variables Control and change the scouting variables on the Scouting tab for runs which
during a run
have not yet been started.

• Settings for the run that is currently in progress cannot be changed.

• You can add more scouting runs to the scheme as long as the last run has not
been started.
• You can use this feature to adjust variables for scouting even if the start protocol
is not displayed at the beginning of each run.

03-0014-90 • p 216
 How to perform method runs 9

9.5 How to perform a MethodQueue run

Instruction The table below describes how to run a MethodQueue:

Step Action
1 • Make sure that all systems used in the MethodQueue are connected
with control mode connections.

2 Select a MethodQueue in the Methods pane in the UNICORN Manager

and
• choose File:Run

or
• right-click the MethodQueue icon in the Methods pane and select
Run from the shortcut menu.

or
• double-click the MethodQueue icon in the Methods pane and click
the Run button in the MethodQueue Editor dialog box.

Result: The MethodQueue will start in accordance with the conditions

defined in the MethodQueue setup.

See 8.1 How to create a new MethodQueue on page 183 for information about how
to create a MethodQueue.

Unattended Meth- The Start Protocol for the first and each subsequent method step in the MethodQueue
odQueue opera- is displayed when the corresponding method is run. If you require unattended
tion
MethodQueue operation after the start of the first method step, make sure that
subsequent method steps do not include a Start Protocol.
Note: If the Start Protocol for a method in the queue is cancelled, the MethodQueue
is paused. Select MethodQueue:Display Running in the UNICORN Manager and Restart
or End the run in the displayed dialog box.

MethodQueues You can choose to place a method in a MethodQueue if the system is already busy
when the system is with another method run (See 8.1 How to create a new MethodQueue on page
busy
183). In a similar manner you can also start a new MethodQueue while another
MethodQueue is in progress. It will be placed in queue and executed when the first
queue is completed.

• p 217
 9 How to perform method runs
9.5 How to perform a MethodQueue run

How to display Definition: A pending MethodQueue is one for which Run has been requested but
and edit pending which has not yet started, either because the system is not available or because the
and running
MethodQueues setup time has not been reached.
The table describes how to display running and pending MethodQueues.

Step Action
1 Click the Running MethodQueue icon.

Result: The Running MethodQueue dialog box is displayed.

2 Select a MethodQueue in the Active MethodQueues list box.

Result: Information on the selected MethodQueue is displayed in the
Details for... field of the dialog box.
Choose from the following:
• The Restart button restarts the currently running MethodQueue if
a Start Protocol has been terminated by Cancel.
• The End button terminates a running MethodQueue after the cur-
rent step. Any methods currently in operation will continue to
run and must be terminated with the End button in the System
Control window if they are not to run to completion. If you click
the End button for a pending MethodQueue, it is deleted from the
pending list.
• The End All button terminates the running MethodQueue and de-
letes all MethodQueues from the pending list.
• The Close button closes the dialog box.

03-0014-90 • p 218
 How to perform method runs 9

9.6 If the network connection fails

Results will be If the results of a method run are stored on a server or other location, and there is
saved in the Failed a network communication failure during a method run that has been started from
folder
a remote station, the method run will continue and the results will be saved in the
Failed folder on the local station. A control mode connection can be established
on the local station to control the running system. See the Administration and
Technical Manual for more details.

• p 219
 10 How to view results

10 How to view results

Introduction A result file is automatically generated at the end of a method run and contains a
complete record of the method run, including method, system settings, curve data
and method run log. The Evaluation module offers extensive facilities for
presentation and evaluation of curve data.
This chapter describes how to present the chromatograms and curves of your result
file and how to create and print reports.

In this chapter This chapter contains these sections:

Topic See
How to open a result file 10.1

How to use the File Navigator 10.2

Basic presentation of chromatograms 10.3

How to optimize the presentation of chromatograms 10.4

How to print active chromatograms 10.5

How to create and print reports 10.6

Run documentation 10.7

03-0014-90 • p 220
 How to view results 10

10.1 How to open a result file

Introduction All contents of the result files are opened in the Evaluation module. By default, the
chromatograms in a run are shown as opened windows. The chromatogram window
on top is the active window. There is also a minimized Temporary chromatogram
window. See 10.3 Basic presentation of chromatograms on page 226 for further
information about chromatograms.
Note: It is not possible to open the same result file from two different locations
simultaneously.

How to open a To open a result file from the UNICORN Manager, do one of the following:
result from the
UNICORN Man- • Double-click a result file in the Results window of the UNICORN Manager,
ager
or
• Select a result file icon in the Results window of the UNICORN Manager and select
File:Open,

or
• Click the Evaluation icon in the UNICORN Manager, open the Evaluation module
and select a result file from the Open Result dialog box.

How to open a To open a result file in the Evaluation module:

result in the Evalu-
ation module • Do the following:
- Select File:Open
- Select a result file from the Open Result dialog box.

or
• Do the following:
- Select View:File Navigator
- Locate and select a result file from the File Navigator.

Note: See 10.2 How to use the File Navigator on page 222 for detailed instructions
on how to locate files and set up File Navigator preferences.

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10.2 How to use the File Navigator

10.2 How to use the File Navigator

Introduction The File Navigator can be used to locate and open result files in the Evaluation
module. Recent runs are also listed based on the user preferences.

How to open the To open the File Navigator:

File Navigator
• Click the Evaluation module icon in the Windows task bar.
• Select View:File Navigator

Result: The File Navigator opens in the Evaluation module. The File Navigator can
be resized and dragged to other positions in the module.

How to open files The table below describes how to use the Files list to locate and open a result file.
from the Files list
Step Action
1 • Click the Files tab

Result: The Files list opens in the File Navigator. The list is identical
to the Results window in the UNICORN Manager and shows all user
available folders and files.

2 • Navigate to the desired folder

• Double-click the desired result file

Result: The result file opens in the Evaluation module.

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How to use Find The Find function in the File Navigator is used to locate result files in the available
to search for files folders. The table below describes how to use the Find function to locate and open
a result file.

Step Action
1 • Click the Find tab

Result: The Find list opens in the File Navigator.

2 • Type a file name or part of a file name in the Result file name text
box. Standard wildcard characters can be used.

or
• Type a Sample ID value in the Value of variable Sample_ID text
box.

Note: The defined variable name must begin with Sample_ ID.

3 • Click the Quick Find button

Result: The located result files are listed in the File Navigator.

4 • Double-click the desired result file or chromatogram

Result: The file or chromatogram opens in the Evaluation module.

Note: Click the Find button to open the Find Files dialog box where more search
functions are available. See 4.3 How to arrange and locate your files on page 74
for more information.

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10.2 How to use the File Navigator

How to open a The Recent Runs list shows all the available recorded recent runs based on the
Recent Run selected user preferences. The table below describes how to use the Recent Runs
list to locate and open a result file.

Step Action
1 • Click the Recent Runs tab

Result: The Recent Runs list opens in the File Navigator.

Note: Until the files and chromatograms in the list have been opened
and saved they are noted in bold text. When they are opened and
saved the text is changed to plain text.

2 • If needed, click the Refresh button in the bottom of the File Nav-
igator

Result: The Recent Runs list is updated with all runs that were per-
formed since the File Navigator was opened the last time.

3 • Locate the desired run

• Double-click the file

Result: The result file opens in the Evaluation module.

Note: Click the + signs to view or select individual chromatograms from the result
files. Individual result files can be selected and removed from the list by clicking
the Remove button. The Remove all button clears the whole list.
Note: Remove only clears the list, the files are not deleted.

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How to set prefer- The File Navigator will display Recent Runs based on the individual user preference
ences for Recent settings. The table below describes how to adjust the preference settings:
Runs

Step Action
1 • Click the Preferences button

Result: The Preferences dialog box opens.

2 • Type the maximum number of files to keep on the list

• Type the maximum number of days to keep the files on the list

3 Select which files to display on the list:

• Only files created by the current user
• All files created by specified users

Note: Click the Specify button to open a dialog box and select from
a list with all accessible users.
• All accessible files regardless of the creator

4 • Choose Remove files when saved if the files are to be removed

from the list when they have been saved.
• Click the OK button.

Result: All new results will be displayed on the Recent Runs list based
on the changed preferences.

How to close the To close the File Navigator:

File Navigator
• Click the small cross in the top right-hand corner of the File Navigator.

Result: The File Navigator closes.

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10.3 Basic presentation of chromatograms

10.3 Basic presentation of chromatograms

Introduction This section describes how to access result files and optimize the presentation of
a chromatogram and its curves via the Chromatogram Layout dialog box.

In this section This section contains these topics:

Topic See
Introduction and temporary chromatograms 10.3.1

The Chromatogram window 10.3.2

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10.3.1 Introduction and temporary chromatograms

Contents of a Chromatograms can be viewed in the Evaluation module.

chromatogram
A chromatogram includes a number of curves that have been created during a
method run, such as UV, conductivity, pH, fraction marks, etc. A chromatogram
also contains the curves created and saved during an evaluation session. The original
raw data curves cannot be deleted or modified, but they can be used as the basis
for evaluation procedures and subsequent creation of new curves.

Temporary chro- A Temporary chromatogram is essentially an empty chromatogram that is specific

matograms to the Evaluation module. It is also user-specific, so that all users have their own.
Information contained within a Temporary chromatogram is automatically saved
from one evaluation session to the next, but is not saved within the result files.

How to copy Curves can be copied into Temporary and comparisons or evaluations can be
curves into Tem- performed. This is particularly useful if you do not want to clutter up your original
porary
chromatograms with a large number of curves. It can also be used to keep blank
run curves or curves to compare when you open different result files.
The table below describes how to copy curves into Temporary:

Step Action
1 Open a result file.

2 Select Edit:Copy:Curves.
Result: The Copy Curve dialog box is displayed.

3 Select a source chromatogram and a curve to be copied in the Source

Chromatogram fields.

4 Select Temporary as the target chromatogram and a position for the

new curve in the Target Chromatogram fields.

5 Click the Copy button.

Result: The curve is copied into the Temporary chromatogram.
Click the Close button.

How to clear a The table below describes how to clear the contents of a temporary chromatogram:
temporary chroma-
togram
Step Action
1 Open the relevant result file.

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10.3 Basic presentation of chromatograms
10.3.1 Introduction and temporary chromatograms

Step Action
2 • Select Edit:Clear Temporary Chromatogram.
• Click the Yes button to confirm.

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10.3.2 The chromatogram window

Main views The chromatogram window is divided into four main views:
• File Navigator
• header information
• curves
• peak and pool tables

The displayed areas for the views can be adjusted by dragging the borders with
the mouse cursor between the views.
The picture below shows an example of the window with all views present:

How to view You can display header information at the top of a chromatogram, with details
header informa- on variables, scouting variables, questions and/or notes. Header information cannot
tion
be displayed for imported chromatograms.
The table below describes how to display header information:

Step Action
1 Open a result file.

2 In the Evaluation module, select Edit:Chromatogram Layout...

Result: The Chromatogram Layout dialog box is displayed.

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10.3 Basic presentation of chromatograms
10.3.2 The chromatogram window

Step Action
3 • Click the Header tab.
• Select the options you want in the header.
• Click OK.

4 • In the chromatogram window, place the cursor at the top of the

curve window (just below the toolbar) until the window sizing
tool appears.
• Drag the cursor down to display the header window.

How to view peak The table below describes how to display peak table information if the result has
table information been integrated:

Step Action
1 Open a result file.

2 • Choose Edit: Chromatogram Layout.

Result: The Chromatogram Layout dialog box opens.

3 • Click the Peak Table tab.

• Select a peak table in the Select peak table to display list.
• Select what peak table columns to display.
• Check if global peak table data should be displayed or not.
• Click OK.

How to view the If fractions are pooled, the Pool Table is displayed in the same pane as the Peak
Pool table Table.
• Click the Pool Table tab to display the Pool Table information.

See 11.5 How to pool fractions on page 279 for more information on how to create
the Pool Table.

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Run curves, de- The first time a result file is opened and viewed, a default layout is applied to
fault appearance display all the original curves. The default layout can be changed by the user (see
and information
10.4.5 How to save and apply a layout on page 242).
Information for each curve
Each curve is automatically assigned a default color and style, with default
information about each curve displayed in the key above the curves. This
information includes
• result file name
• chromatogram name
• curve name.

Choose the Y-axis scale

Each curve has a correspondingly colored Y-axis. To choose the appropriate Y-axis
scale
• click on the Y-axis until the desired scale is displayed

or
• click on the name of the curve.

Run curves, short- When viewing curves in the Evaluation module, you can access a menu that provides
cut menu a quick alternative to menu commands. Right-click the run curves view to display
the menu shown in the picture below:

Optimizing the The chromatogram window can be minimized and maximized using ordinary
workspace Windows commands. The table below describes extra features to optimize the
workspace:

Use the command if you want...

Window:Arrange icons to arrange icons of minimized windows.

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10.3 Basic presentation of chromatograms
10.3.2 The chromatogram window

Use the command if you want...

Window:Tile to view several chromatogram windows side by side.

Window:Cascade to stack the open windows like a deck of cards.

How to display a The table below describes how to display a vertical marker line:
vertical marker
line
Step Action
1 Right-click the Curves pane and select Marker.

2 Drag the marker line with the mouse.

Result: Where the line bisects the curve, the X-axis and Y-axis values
are displayed at the top right corner of the pane.

Note: Right-click and select Snapshot to record the marker position values. See
2.2.7 Snapshots on page 41 for more information about the Snapshot function.

How to set a refer- The table describes how to set a reference point:
ence point
Step Action
1 • Display a Marker in the Curves pane.
• Right-click and select Set Marker Ref. Point to define a reference
point for the marker position.

2 When the marker is moved from the reference point, the X-axis and
Y-axis values for the new position are displayed together with:
• the new position in relation to the reference point,
• the minimum, maximum and average values for the curve interval
between the reference point and the new position.

How to display The table below describes how to display the logbook entries as an overlay in the
the logbook over- chromatogram.
lay

Step Action
1 • Right-click in the chromatogram window and choose Properties
on the shortcut menu.

Result: The Chromatogram Layout dialog box opens.

2 • Choose the Curve tab.

• Select the Logbook curve.

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Step Action
3 • Choose the Curve Style and Color tab.
• Click the Filter button in the Logbook text alignment field.

Result: The Filter Logbook dialog box opens.

4 • Select all the logbook items you want to display and click OK.
• Click OK in the Chromatogram Layout dialog box.

Result: The selected logbook items are displayed in the chromato-

gram window.

How to view the complete logbook information

At some breakpoints there can be more logbook information than what is possible
to conveniently display in the chromatogram window. The additional information
that is not displayed is indicated by an arrow point symbol by the break point.
• Hold the mouse cursor over the break point to display the complete information
in a flyover text box, as shown in the illustration below:

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10.4 How to optimize the presentation of a chromatogram

10.4 How to optimize the presentation of a chromatogram

Introduction This section describes some of the ways you can optimize the presentation of a
chromatogram.

In this section This section contains these sub-sections:

Topic See
How to make changes in the Chromatogram Layout dialog box 10.4.1

The Curve tab and Curve names tab 10.4.2

The Curve Style and Color tab 10.4.3

How to change and fix the axes 10.4.4

How to save and apply a layout 10.4.5

How to show part of a curve 10.4.6

How to change the size of Fraction, Injection and Logbook marks 10.4.7

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10.4.1 How to make changes in the Chromatogram Layout

dialog box

Instruction The Chromatogram Layout dialog box is used to make changes regarding
chromatogram presentation. The main features of the Chromatogram Layout dialog
box regarding chromatograms are described in the subsequent sections in this
chapter. Features regarding peak tables are described in 12.1.2 How to perform a
peak integration on page 330.
The table below describes how to make changes in the Chromatogram Layout dialog
box:

Step Action
1 Open a result file.

2 • Right-click the chromatogram window and select Properties

or
• Choose Edit:Chromatogram Layout.

Result: The Chromatogram Layout dialog box is displayed. The view

from which you activate the Properties command determines the tab
that is displayed in the Chromatogram Layout dialog box.

3 Carry out the changes on the different tabs to get the desired layout
for header, curves and peak table.
Select Apply to all chromatograms if you want to apply changes made
in the Chromatogram Layout dialog box to all open chromatograms.
Click OK.

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10.4 How to optimize the presentation of a chromatogram
10.4.2 The Curve tab and Curve Names tab

10.4.2 The Curve tab and Curve Names tab

The Curve tab The Curve tab of the Chromatogram Layout dialog box contains a list of all the curves
included in the chromatogram. Select the curves you want to display in the
chromatogram, and click OK.

Curve name ap- You select options for the curve name appearance on the Curve Names tab. This is
pearance an example of a default curve name:
Result:11_UV1_280
The table below describes the three components that make up the default curve
name:

Component Description Example

Result name Name of the result. Result

Chromatogram name Number given automat- 11

ically during a run or a
name defined by the
New_Chromatogram in-
struction.

Curve name Curve type, for example UV1_280

detection of an eluted
component.
In this example, the sys-
tem uses a variable
wavelength detector, so
the wavelength (280) for
the UV curve is also giv-
en.

How to choose You can choose to view only part of the curve name. The table below describes
curve name ap- how to do this:
pearance

Step Action
1 Open a result file.

2 Choose Edit:Chromatogram Layout.

Result: The Chromatogram Layout dialog box is displayed.

3 Click the Curve Names tab.

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Step Action
4 • Select the appropriate boxes for Curve name appearance.
• Select the appropriate Curve legend position.
• Click OK.

Note: It is usually sufficient to select the Curve Name option if only one
chromatogram is being evaluated. However, confusion can arise when more than
one chromatogram is shown, so more complete names might be necessary.

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10.4 How to optimize the presentation of a chromatogram
10.4.3 The Curve Style and Color tab

10.4.3 The Curve Style and Color tab

Introduction All curves within a chromatogram are represented by a default color and line style.
Curves imported into the chromatogram or newly created curves are automatically
assigned a color and line style.

Peak label settings Peaks can be labeled on the Curve Style and Color tab of the Chromatogram Layout
dialog box. Use a combination of the following labels:
• Retention (the default label)
• sequential Number
• user-defined Peak name.

Fraction text and Both Fraction text and Logbook text can be set to the following alignment options:
Logbook text
alignment settings • Vertical
• Horizontal
• Fly Over, which sets text labels as hidden text that appears only when the cursor
is carefully positioned over a fraction mark.

How to change The table below describes how to change the color and style of a curve:
the color and style
of a curve
Step Action
1 Open a result file.

2 Choose Edit:Chromatogram Layout.

Result: The Chromatogram Layout dialog box is displayed.

3 Click the Curve Style and Color tab.

4 • Select the curve you want to change from the list.

• Select the desired color and style.
• Click OK.

How to display The table below describes how to display and filter logbook curve information:
and filter logbook
information
Step Action
1 Open a result file.

2 • Choose Edit:Chromatogram Layout.

Result: The Chromatogram Layout dialog box is displayed.

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Step Action
3 • Click the Curve tab.
• Select the logbook curve.

4 • Click the Curve Style and Color tab.

• Click the Filter... button in the Logbook text alignment field.

Result: The Filter Logbook dialog box is displayed.

5 • Select the type of logbook information you want to show.

• Set the maximum block depth to show.
• Click OK.

How to display a The table below describes how to display a hatched background in the
hatched back- chromatogram window:
ground

Step Action
1 Open a result file.

2 • Choose Edit:Chromatogram Layout.

Result: The Chromatogram Layout dialog box is displayed.

3 • Click the Curve Style and Color tab.

• Select the Hatch box.
• If desired, select the Apply to all chromatograms box and click OK.

Result: Hatch marks are displayed as a background.

Note: You can also right-click in the Chromatogram window and select Hatch.

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10.4 How to optimize the presentation of a chromatogram
10.4.4 How to change and fix the axes

10.4.4 How to change and fix the axes

How to change The table below describes how to change and fix the Y-axis:
and fix the Y-axis
Step Action
1 Open a result file.

2 Choose Edit:Chromatogram Layout.

Result: The Chromatogram Layout dialog box is displayed.

3 Click the Y-Axis tab.

4 • Select the appropriate curve from the list.

• Click the Fixed option.

5 • Type the desired minimum and maximum values.

• Click the All with this unit button if you want other curves with
the same Y-axis units as the current scaled curve to be similarly
scaled.
Note: The values will only be applied to existing curves. They
will not be applied to new curves created after this function was
last used.
• Click the appropriate Pressure unit (MPa, psi, bar) option to
change Y-axis units for pressure curves.
Note: Default Pressure unit is From strategy, which is the unit
defined in the original run strategy.
• Click OK.

How to add a The table below describes how to add a second Y-axis to the chromatogram.
second Y-axis
Step Action
1 Choose Edit:Chromatogram Layout.
Result: The Chromatogram Layout dialog box is displayed.

2 Click the Y-Axis tab.

3 • Select the appropriate curve from the Right Axis droplist.

• Click the OK button.

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How to change The table below describes how to change and fix the X-axis:
and fix the X-axis
Step Action
1 Open a result file.

2 Choose Edit:Chromatogram Layout.

Result: The Chromatogram Layout dialog box is displayed.

3 Click the X-Axis tab.

4 Select the appropriate option in the Base field:

• Time of retention
• Volume
• Column Volume

Note: Some calculated curves, for example baselines, exist in only

one base and might seem to disappear when the base is changed.
Curves are collected in time and recalculated for display in volume.
Thus, switching the base between Time and Volume can slightly alter
the resolution.

5 • Click the Fixed option in the Axis scale field to set the axis limits
manually.
• Type the desired minimum and maximum values.
• If desired, de-select the Adjust retention zero to injection number
checkbox.
This checkbox is selected by default. The function sets the
time/volume to zero at the injection mark, that is when the sample
was injected. The time and volume before injection will become
negative values.
• Click OK.

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10.4 How to optimize the presentation of a chromatogram
10.4.5 How to save and apply a layout

10.4.5 How to save and apply a layout

Introduction All configurations that you make in the Chromatogram Layout dialog box can be
saved as a layout. It is possible to apply saved layouts to other chromatograms.
All saved layouts are user-specific.

How to save a The table below describes how to save a layout:

layout
Step Action
1 Open a result file.

2 Choose Edit:Chromatogram Layout.

Result: The Chromatogram Layout dialog box is displayed.

3 Make the appropriate layout configuration within the various tabs.

View your changes
Click OK if you want to return to the chromatogram window to see
the applied affects of a given configuration. Return to the Chromato-
gram Layout dialog box to perform further changes.

4 • Select the Layout Library tab.

• Click the Save current layout as button.
Result: The Save Layout dialog box is displayed.

5 • Type a name for the layout.

• If you want the current layout to be the new default layout, select
the Save as default option.
• Click OK.
Result: The new name is added to the Saved layouts list.
• Click OK.

How to apply a The table below describes how to apply a layout:

layout
Step Action
1 Select the Layout Library tab on the Chromatogram Layout dialog box.

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Step Action
2 • Select a layout from the Saved layouts list.
• Click the Apply selected layout button.
Result: The layout is automatically applied to the active chroma-
togram window.
• If the same layout is to be applied to all chromatograms on the
Evaluation workspace, select the Apply to all chromatograms
checkbox.
• Click OK.

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10.4 How to optimize the presentation of a chromatogram
10.4.6 How to show part of a curve

10.4.6 How to show part of a curve

Introduction You can select a part of a curve in order to examine details more closely.
You can
• use the zoom to magnify

or
• cut the axes.

It is also possible fix the axes, see 10.4.4 How to change and fix the axes on page
240.

How to use the In the active chromatogram window, you can zoom in on a designated area of the
zoom function chromatogram. This is the easiest and quickest way to enlarge different parts of a
curve. The table below describes how to do this:

Step Action
1 Open a result file.

2 • Place the mouse pointer in any corner of the area you want to
magnify.
• Press and hold the left mouse button. A magnifying glass icon
will be added to the mouse pointer arrow on the screen.
• Drag a box to cover the area to be magnified, and release the
mouse button.

Result: The selected region is now displayed in the entire chromato-

gram window, together with appropriate scales for the Y and X
axes.

3 Use the arrow keys on the keyboard to move around in the chroma-
togram at the current zoom scale.

4 Undo zoom
Right-click in the window and select Undo zoom to undo the last
zoom step.
Reset zoom
Right-click in the window and select Reset zoom to reset all zoom
steps at once.

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How to cut a The table below describes how to cut the curve between two values on the X-axis
curve and store as and store this part of the curve as a new curve:
a new curve

Step Action
1 Open a result file.

2 Choose Operations:Cut curve.

Result: The Select Curve(s) to Operate On dialog box opens.

3 • Select the curves to be operated on.

• Click OK.

Result: The selected curves are shown in the Cut dialog box which
contains two vertical cursor lines.

4 To select the region to be cut, either

• drag the two cursor lines to define the left and right limits of the
cut area

or
• type the desired left and right limit values in the Left limit and
Right limit boxes.

Note: The areas outside of the Left limit and Right limit will not be
saved in the newly created cut curve. Thus, the X-axis of the new
curve will not begin at zero unless this is designated as one of the
limits. The original curve is not changed.

5 Click OK.
Result: The Save Cut Curves dialog box opens.

6 • Select whether to save the new cut curve in

- the Source chromatogram, that is the current active chromato-
gram,
or
- a New chromatogram (if you select this option, you can change
the name of the chromatogram. Note that it is a recommend-
ation not to use only numbers as names for chromatograms.).
• Click OK.
Result:
- If the destination of the cut curve was the source chromato-
gram, the cut curve is automatically displayed in the source
chromatogram.
- If the destination of the cut curve was a new chromatogram,
this will be represented as a new, open chromatogram window.

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10.4 How to optimize the presentation of a chromatogram
10.4.7 How to change the size of Fraction, Injection and Logbook marks

10.4.7 How to change the size of Fraction, Injection and

Logbook marks

Introduction The sizes of Fraction, Injection and Logbook marks are all determined by your user
settings. The settings are applied for all your chromatograms.

Instruction The table below describes how to change the size of the Fraction, Injection and
Logbook marks:

Step Action
1 • Choose Administration:Change User Attributes in the UNICORN
Manager module.

Result: The Change user attributes dialog box opens.

2 Select the unit for the Fraction mark height:

• Percent of window height
• Character Heights
• Pixels

Type a new size value in the Fraction mark heightbox.

3 • Repeat step 2 for the Injection and Logbook marks if necessary.

• Click OK.

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10.5 How to print active chromatograms

Introduction This section describes how to print the chromatograms that are open in the
Evaluation module.

The Print Chroma- This is an illustration of the Print Chromatograms dialog box.
tograms dialog
box Note: The selected print format is outlined in red.

Instruction The table below describes how to print active chromatograms.

Step Action
1 Open all chromatograms that you want to print in the Evaluation
module.

2 • Select File:Print.

or
• Click the Print toolbar icon.

Result: The Print Chromatograms dialog box opens.

3 Select print format and layout options.

4 • Click OK to print.

or
• Proceed with step 5 to preview and edit the layout.

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10.5 How to print active chromatograms

Step Action
5 Click the Preview button.
Result: The Customise Report window opens.

6 • Click the Edit Mode button to make changes, e.g. change the order
of the chromatograms (see 10.6.1 How to create and print a
customized report on page 250 for more information about how
to edit).
• Click the Preview button to return to preview mode.

7 • Select File:Print.

or
• Click the Print toolbar icon.

Result: The Print dialog box opens.

8 • Select the print range and number of copies.

• Click OK.

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10.6 How to create and print reports

Introduction The Evaluation module provides extensive tools to create detailed reports. This
section describes how to create and print reports that are based either on a standard
or a customized layout.

In this section This section contains these topics.

Topic See
How to create and print a customized report 10.6.1

How to create and print a standard report 10.6.2

How to edit an existing report format 10.6.3

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10.6 How to create and print reports
10.6.1 How to create and print a customized report

10.6.1 How to create and print a customized report

Introduction You can choose from a variety of objects to include in a report, including
chromatograms, methods, documentation, free text and more in the customized
report interface. You can also place, align and size the objects as you please. This
section describes how to create a customized report format.
Should you need to store store your reports in an electronic format you can save
them as PDF files. This section also describes how to do this..

How to open the The table below describes how to open the Report Editor in Edit mode to create a
Report Editor in customized report format.
edit mode

Step Action
1 Open a result file in the Evaluation module.

2 • Select File:Report.

or
• Click the Report icon.

Result: The Generate Report dialog box opens.

3 • Click the New button.

Result: The Create New Report Format dialog box opens.

4 • Select the Customised format and click OK.

Result: The Report Editor opens in Edit mode.

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 How to view results 10

The Edit mode The illustration below shows the Report Editor window in Edit mode with a blank
window report open:

Toolbar button The table below describes the different functions of the Edit mode toolbar buttons
functions in the in the Report Editor:
Report Editor

Toolbar button Function

Preview/Edit This button toggles between a print preview of the
report and the Edit mode.

Next Page This button displays the next page or pair of pages
(where there are more than one page).

Prev Page This button displays the previous page or pair of

pages (where there are more than one page).

One Page/Two Pages This button toggles between single page view and
pairs of pages view, when there is more than one
page.

Zoom In This button increases the magnification of the view.

Zoom Out This button decreases the magnification of the view.

Add Page This button adds a blank page to the report.

Delete Page This button deletes the current page from the report.

Exit This button closes the Customize Report window.

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10.6 How to create and print reports
10.6.1 How to create and print a customized report

How to add and The table below describes how to add or delete report pages in the Report Editor:
delete report pages
If you want... then...
to add new pages, • click the Add Page toolbar button.

Result: A new page is added after the last page.

to delete a page while • select the page with Next Page or Prev Page,
in One Page mode, • click the Delete Page toolbar button and confirm
the deletion.

to delete a page in Two • select the page with Next Page or Prev Page,
Page mode, • click an object on the page,
• click the Delete Page toolbar button and confirm
the deletion.

How to change The page layout is changed in the Page Setup dialog box. The table below describes
the page layout how to set up the page layout:

Step Action
1 Double-click anywhere on the report page in the Report Editor (not
on an object).
Result: The Page Setup dialog box opens.

2 • Type new values for the Margins if necessary.

• Select the appropriate Settings and Unit.

Note: An extra Header tab will appear if you de-select the option to
have the same header on all pages. The First Header tab is used for
the first page header only, and the Header tab is used for all sub-
sequent pages.
• Click the First Header tab.

3 • Select all the items you want to include in the header from the
Select Items list.
• Click the Font button to change the font for all items if necessary.

4 • Type header text in the Free text box and click the Font button to
alter the default font if necessary.
• Type the report title in the Report title box and click the Font
button to alter the default font if necessary.

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Step Action
5 • Select the Logo check box and click the Browse button if you want
to locate and select a logo image file.
• Select the Alignment for the logo, if necessary.

Note: The logo file must be in bitmap format (.bmp) and smaller
than 64 kB. Larger logo files or files in other formats must be inserted
as Picture objects.

6 If you want to have a line under or over the header, select the appro-
priate option in the Layout field.

7 • Repeat steps 3 to 6 on the Footer tab and the subsequent pages

Header tab.

Note: All Header and Footer tabs contain the same options. You can
have all information in either the header or footer or split informa-
tion between the header and footer as required.
• Click OK.

How to add ob- The table below describes how to add objects to the report. The various objects
jects to the report are described below this table.

Step Action
1 • Click the appropriate icon in the Report items toolbar.

or
• Choose an object from the Insert menu.

2 • Press and hold the left mouse button on the report page, and drag
out a box to the size of the item you want to insert.

Note: The mouse pointer shows a symbol for the type of item you
have selected.
• Release the mouse button.

Result: A Setup dialog box opens. The dialog is specific to the type
of item that you want to insert.

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10.6 How to create and print reports
10.6.1 How to create and print a customized report

Step Action
3 • Select the desired options and click OK.

Result: The object is inserted onto the page.

Note: If you want to edit an object later, double-click the object box.

How to add free The table below describes how to add free text to the report:
text
Step Action
1 • Click the Free Text icon.

• Press and hold the left mouse button on the report page and drag
out a box to the size of the text. Release the button.

Result: The Setup Free Text dialog box opens.

2 • Type text in the edit field.

• Select if the text is to start on a new page.
• Select if the text box should be automatically sized.
• Select if the text should appear in the same position on all pages,
for example as header and footer text.

3 • Click the Font button to change the default font.

Result: The Font dialog box opens.

• Make the necessary changes and click OK to return.

• Click OK.

Result: The text object is inserted onto the page.

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How to add a pic- The Picture dialog box is useful to insert logos, pictures or other figures in the
ture report. The table below describes how to add a picture object to the report:

Step Action
1 • Press and hold the left mouse button on the report page and drag
out a box to the size of the picture item. Release the mouse button.
• Click the Picture icon.

Result: The Picture dialog box opens.

2 • Click the Browse button to locate the desired picture file.

• Select the picture file and click the Open button.

Note: The file formats .bmp, .emf, .jpg and .tif can be used.
Result: A preview of the selected picture is displayed.

3 • Select the desired Settings and click OK.

Result: The picture is inserted onto the page.

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 10 How to view results
10.6 How to create and print reports
10.6.1 How to create and print a customized report

How to add a The table below describes how to add a chromatogram to the report. The layout
chromatogram or can also be defined to include a peak or pool table if desired.
peak table

Step Action
1 • Click the Chromatogram icon.

• Press and hold the left mouse button on the report page and drag
out a box to the size of the chromatogram. Release the mouse
button.

Result: The Setup Chromatogram dialog box opens.

2 Select which chromatogram(s) to insert from the Selected chromato-

gram(s) droplist.
• Active chromatogram inserts the chromatogram that currently is
active in the Evaluation module.
• All chromatograms inserts all chromatograms that are open in the
Evaluation module.
• 1, 2...etc. inserts the corresponding chromatogram.

3 • Select the desired Settings.

• If desired, change the Fonts.

Note: Separate fonts can be selected for the Chromatogram, the Peak
table and the Header text.

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 How to view results 10

Step Action
4 • Click the Define button in the Layout field if you want to re-define
the layout of the chromatogram.

Result: The Report Chromatogram Layout dialog box opens.

• Make the appropriate changes and click OK to return to the Setup
Chromatogram dialog box.

Note: The changes that you make will only affect the report and not
the view of the chromatograms in the Evaluation module.

5 Click OK.
Result: The chromatogram is inserted onto the page.

Note: All curves can be de-selected in the Report Chromatogram Layout dialog box
leaving only the selected peak table(s) in the report.

How to include a The table below describes how to include a method in the report:
method
Step Action
1 • Click the Method icon.

• Press and hold the left mouse button on the report page and drag
out a box to the size of the item. Release the button.

Result: The Setup Method dialog box opens.

2 Select the items to be included in the report:

• Main Method is the method on which the run was based.

• Blocks are the blocks that were used in the method.

3 • Select the appropriate Settings.

Note: Expand main displays the expanded method view.

• If desired, change the Fonts.
• Click OK.

Result: The method object is inserted onto the page.

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 10 How to view results
10.6 How to create and print reports
10.6.1 How to create and print a customized report

How to add docu- The table below describes how to add documentation to the report:
mentation
Step Action
1 • Click the Documentation icon.

• Press and hold the left mouse button on the report page and drag
out a box to the size of the item. Release the button.

Result: The Setup Documentation dialog box opens.

2 Select the items to be included in the report:

• Select All includes all items in the report.

• Clear All removes all selections.

3 • If desired, change the Fonts.

• Select if the documentation should start on a new page.
• If Select All, Logbook or Run summarySelect All or Logbook was
selected, make the necessary changes to the Base and Logbook
filter settings.
• Click OK.

Result: The selected documentation items are inserted into the report.

How to add the The table below describes how to add the Evaluation Log to the report:
Evaluation Log
Step Action
1 • Click the Evaluation Log icon.

• Press and hold the left mouse button on the report page and drag
out a box to the size of the item. Release the mouse button.

Result: The Setup Evaluation Log dialog box opens.

2 • If desired, change the Fonts.

• Select if the Evaluation Log should start on a new page.
• Click OK.

Result: The Evaluation Log is inserted into the report.

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How to include The table below describes how to include Quantitate and Molecular Size data in the
Quantitate and report.
Molecular Size
data Note: This option is only available if the Analysis module has been installed.

Step Action
1 • Click the Quantitate and Mol Size icon.

• Press and hold the left mouse button on the report page and drag
out a box to the size of the item. Release the mouse button.

Result: The Setup Quantitate dialog box opens.

2 • If desired, change the Fonts.

• The default option is that the Quantitate and Molecular Size data
will start on a new page.
• Click OK.

Result: The Quantitate and Molecular Size data is inserted into the
report.

How to include The table below describes how to include Frac-950 data in the report.
Frac-950 data
Note: This option is available only if a Frac-950 has been installed and if the result
file contains data from the Frac-950.

Step Action
1 • Click the Frac-950 icon.

• Press and hold the left mouse button on the report page and drag
out a box to the size of the item. Release the mouse button.

Result: The Setup Frac-950 dialog box opens.

2 • If desired, change the Fonts.

• Select if the Frac-950 data should start on a new page.
• The Include rack layout option is selected by default. This will
display the rack layout that was used in the run.
• Click OK.

Result: The Frac-950 data is inserted into the report.

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 10 How to view results
10.6 How to create and print reports
10.6.1 How to create and print a customized report

How to move and The table below describes how to select, move and resize objects freely:
resize objects
freely
If you want... then...
to select a single object, • click the Select icon,

• click the object of interest.

to select several ob- • click the Select icon,

jects, • press and hold the <Ctrl> key while you click the
objects.

to move the selected click on the objects, hold down the left mouse button
object(s), and drag the object(s) to the new position.

to resize the selected click one of the object border anchors, either in the
object(s), corners or in the middle of a border, and drag the
box to the new size.
Note: Some Text objects cannot be resized.

Alignment toolbar Objects can be placed in exact positions and sized in relation to other objects. The
icon functions table below describes the function of the Alignment toolbar icons in the Report
Editor:

Tool- Function
bar
icon
Align left
Matches the left alignment of all selected objects to that of the
highlighted object.

Align right
Matches the right alignment of all selected objects to that of the
highlighted object.

Align top
Matches the top alignment of all selected objects to that of the
highlighted object.

Align bottom
Matches the bottom alignment of all selected objects to that of the
highlighted object.

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Tool- Function
bar
icon
Adjust to margins
Stretches the selected object(s) to the left and right margins.

Adjust to left margin

Adjusts the selected object(s) to the left margin.

Adjust to right margin

Adjusts the selected object(s) to the right margin.

Adjust to centre
Adjusts the selected object(s) to the center of the page.

Make same size

Adjusts the selected objects to the same size as the highlighted refer-
ence object.

Make same width

Adjusts the selected objects to the same width as the highlighted
reference object.

Make same height

Adjusts the selected objects to the same height as the highlighted
reference object.

Note: The Make same size and Make same width functions can only be used to
resize the width of chromatograms, free text and picture objects.

How to print the The table below describes how to print the report:
report
Step Action
1 • Choose File:Print.

or
• Click the Print icon.

Result: The Print dialog box opens.

Note: Printers are set up in the File menu of the UNICORN Manager.

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10.6 How to create and print reports
10.6.1 How to create and print a customized report

Step Action
2 • Select the printing range.
• Select the number of copies.
• Click OK.

Note: You can also print the report from the Generate Report dialog box.

How to save the The table below describes how to save the finished report as a PDF file:
report in PDF
format
Step Action
1 • Click the UNICORN Manager icon on the Windows taskbar.

Result: The UNICORN Manager opens.

• Choose File:Printer Setup.

Result: The Print Setup dialog box opens.

2 • Select an Adobe Acrobat printer from the Printer Name list (e.g.
Acrobat Distiller).
• Click the Properties button and edit the document properties if
needed.
• Select the appropriate paper size and orientation.

• Click OK.

3 • Click the Evaluation icon on the Windows taskbar.

Result: The Evaluation module opens

4 • Print the report as described in "How to print the report".

Result: The report is created as a PDF file and saved in the location
specified in your Acrobat settings.

Note: You must have a full installation of Adobe Acrobat or a suitable printer
driver to be able to do this.

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How to save the The table below describes how to save the finished report format:
report format
Step Action
1 • Choose File:Save.

or
• Click the Save icon.

Result: The Save Report Format dialog box opens.

2 • Type a name for the format.

• Select if you want to save the format for global use.
• Select if you want to save the format as default.

Note: The name for the default format will automatically be changed
to DEFAULT.
• Click OK.

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10.6 How to create and print reports
10.6.2 How to create and print a standard report

10.6.2 How to create and print a standard report

How to create a You can only select a number of pre-formatted items when you create a Standard
Standard report report format. If you want to edit the layout in detail you must create a Customized
report format. See 10.6.1 How to create and print a customized report on page
250.
The table below describes how to create and save a Standard report format:

Step Action
1 Open a result file.

2 • Select File:Report.

or
• Click the Report icon.

Result: The Generate Report dialog box opens.

3 Click the New button.

Result: The Create New Report Format dialog box opens.

4 • Select Standard format and click OK.

Result: The Create Standard Report Format dialog box opens.

The illustration below shows the Create Standard Report Format dialog
box with the Header tab selected:

5 Click the appropriate tabs and select the check boxes for each item
that you want to include in the report.

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 How to view results 10

Step Action
6 Click the Chromatogram tab and select the chromatogram(s) you
want to include.
• Select the Current option in the Layout field to apply the current
layout in the Evaluation module.

or
• Click the Define button in the Layout field to open the Curve tab
in the Report Chromatogram Layout.
- Select the curves that you want to include in the report and
click OK.

7 • Click the Contents tab to see a list of all the selected items.
• Click the Preview button to see the entire report layout.
• Click the Close button to return.
• Click the Print button to print a test report.

8 • Click the Save As button.

Result: The Save Report Format dialog box opens.

• Type a name in the Report format name text box.
- Select the Save as global format check box to make the format
available to other users.
- Select the Save as default report format check box if desired
(The format is saved as DEFAULT).
• Click OK.

Result: The Generate Report dialog box opens again. The new report
is saved and available in the Format list.

9 • Click the Close button

or
• Click the New button to create another Standard report.

How to print a The table below describes how to print a Standard report format in the Evaluation
standard report module.

Step Action
1 Open a result file.

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 10 How to view results
10.6 How to create and print reports
10.6.2 How to create and print a standard report

Step Action
2 • Select File:Report.

or
• Click the Report icon.

Result: The Generate Report dialog box opens.

3 • Select a Standard report format.

Note: The contents of a Standard report format is displayed in the

Contents field.
• Verify in the Contents field that the report format contains all the
elements that you want to include. Click the Edit button to
modify the report format if needed.

4 • Click the Print button.

Result: The Print dialog box opens.

• Choose what pages and how many copies to print.
• Click OK.

Note: Printers are set up in the File menu of the UNICORN Manager.

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10.6.3 How to edit an existing report format

Introduction This section describes how to edit an existing report format.

How to edit a The table below describes how to edit a standard report format in the Evaluation
standard report module.

Step Action
1 Open a result file.

2 • Select File:Report.

or
• Click the Report icon.

Result: The Generate Report dialog box opens.

3 • Select a Standard report format to edit.

• Click the Edit button.

Result: The Edit Report Format dialog box opens.

• Select Standard format and click OK.

Result: The Edit Standard Report Format dialog box opens.

4 Click the appropriate tabs and select the check boxes for each item
that you want to include in the report format.
Note: See 10.6.2 How to create and print a standard report on page
264 for more information.

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 10 How to view results
10.6 How to create and print reports
10.6.3 How to edit an existing report format

Step Action
5 Click the Contents tab to see a list of all the selected items.

6 • Click the Save As button.

Result: The Save Report Format dialog box opens.

• Type a name in the Report format name text box.
- Select the Save as global format check box to make the format
available to other users.
- Select the Save as default report format check box if desired
(The format is saved as DEFAULT).
• Click OK.

Result: The Generate Report dialog box opens again. The new report
format is saved and available in the Format list.

7 • Click the Close button.

or
• Click the Edit button to edit another report format.

How to edit a cus- The table below describes how to edit a customized report format in the Evaluation
tomized report module.

Step Action
1 Open a result file.

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 How to view results 10

Step Action
2 • Select File:Report.

or
• Click the Report icon.

Result: The Generate Report dialog box opens.

3 • Select a Customized Report Format to edit.

• Click the Edit button.

Result: The report format opens in the Report Editor.

4 • Double-click the report item you want to edit.

• Make the desired changes in the dialog box.
• Continue to edit all items until the format is complete.

Note: See 10.6.1 How to create and print a customized report on

page 250 for more information.

5 • Select File:Save As.

Result: The Save Report Format dialog box opens.

• Type a name in the Report format name text box.
- Select the Save as global format check box to make the format
available to other users.
- Select the Save as default report format check box if desired
(The format is saved as DEFAULT).
• Click OK.

Result: The new report format is saved and available in the Format
list.

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 10 How to view results
10.7 Run documentation

10.7 Run documentation

Introduction The full documentation for a method run is stored in the result file. This section
describes:
• some of the contents of the run documentation,
• how to view and print the run documentation,
• how to save the method from the run as a new method.

How to view and The table below describes how to view and print the run documentation.
print the run docu-
mentation
Step Action
1 Open a result file.

2 • Choose View: Documentation in the Evaluation module.

or
• Click the view Documentation icon.

Result: The Documentation dialog box opens.

See further information about some of the tabs below.

3 • Click the Print button.

Result: The Print dialog box opens.

• Select the documentation items you want to print and click OK.

The tabs of the The table below describes the contents of some of the Run Documentation tabs.
Documentation
dialog box
Documentation tab Contents
Variables The Variables tab lists the parameters that were used
during the method run.

Scouting The Scouting tab displays the whole scouting scheme,

with the values for the current result file displayed in
yellow cells.

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 How to view results 10

Documentation tab Contents

Notes The Notes tab displays the notes that you have made
at various times during the method run. You are also
able to type new comments on the Evaluation Notes
sub-tab.
Note: Click the Find button to search for a specific
text string in the Notes.

Calibration The Calibration tab displays the system calibrations

and when and by whom they were made.

Logbook The Logbook tab displays what happened during a

method run. You can view information concerning
alarms, the method, manual changes during the run,
errors, etc.
Note: Click the Find button to search for a specific
text string in the Logbook.

Evaluation Log The Evaluation Log lists all of the evaluation opera-
tions that you have performed for the result file dur-
ing all sessions, including at the end of the method.

Method Information The Method Information tab displays information about

the method, such as the method name, the target
system and the date of the last change. Information
about the strategy includes name, date and size. There
is also a sub-tab for Signatures.

Frac-950 The Frac-950 tab displays the setup parameters for

the fraction collector provided it is included in the
strategy.

Result Information See "The Result Information tab" in this section.

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10.7 Run documentation

The Result Inform- The Result Information tab displays information about the result file, such as
ation tab
• the result file name
• the system that was used
• the last date it was changed.

Information about the strategy includes name, date and size. The Run Summary
sub-tab is a summary of the run expressed in volume or time per block. There is
also a sub-tab for Signatures and a sub-tab where all Snapshots that have been
taken during the run are displayed.

Save the method You can save the method and the variables that were used for the run as a new
used for the run as method:
a new method

Step Action
1 • Select the Text Method tab in the Documentation dialog box.
• Click the Save as button.

Result: The Save As dialog box opens.

2 • Select the appropriate destination folder.

• Type a name in the Method name text box.
• Select a system in the For System field.
• Select a technique in the Technique field.
• Click OK.

Result: The method is saved.

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 How to edit results 11

11 How to edit results

Introduction This chapter describes

• how to edit the results that are presented in the Evaluation module
• how to import and compare runs
• how to import and export results.

For more information about how to view results, see chapter 10 How to view
results on page 220.

In this chapter This chapter contains these sections:

Topic See
How to reduce noise and remove ghost peaks 11.1

How to subtract a blank run curve 11.2

How to add curves 11.3

How to enter and edit text in the chromatogram 11.4

How to pool fractions 11.5

How to match protein activity to a curve 11.6

How to rename chromatograms, curves and peak tables 11.7

How to import and compare different runs 11.8

How to import and export results 11.9

How to sign results electronically 11.10

How to save results and exit the Evaluation module 11.11

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 11 How to edit results
11.1 How to reduce noise and remove ghost peaks

11.1 How to reduce noise and remove ghost peaks

Introduction Sometimes the chromatograms contain curves with a noisy baseline. The noise can
be caused by several factors, for example a dirty flow cell, air bubbles, electrical
noise, dirty buffers, etc. The amount of noise can usually be reduced by taking
proper precautions, for example filtration of buffers and instrument maintenance.
You can also use the smoothing function to reduce or remove background noise
from a selected curve. Smoothing is always a compromise between noise removal
and preservation of peak shape.

How to smooth a The table below describes how to select a smoothing function and smooth a curve:
curve
Step Action
1 Select Operations:Smooth.
Result: The Smooth dialog box is displayed.

2 Select the curve to be smoothed and its target destination.

3 Select the Filter type to be applied. The options are:

• Moving average. Use this if you have noise along most of the curve.
It affects peak height but not retention. There is little effect on
the peak area.
• Autoregressive. Use this if you have periodic noise along the whole
curve. It affects peak height and retention, although this has little
effect on the peak area.
• Median. Use this if there is only one or a few noise spikes, for
example caused by air bubbles, or if the noise is confined to only
a small part of the curve. It can flatten peaks and affect peaks
areas slightly, but does not affect retention.
• Savitzky-Golay. Use this to calculate the smoothing and differenti-
ation of data by a least squares technique.

4 Select an appropriate smoothing parameter value from Light to Hard

for the selected filter in the Filter Parameters field. Use the slider, or
insert a value manually in the text field. The smoothing effect in-
creases with increasing parameter values.
• Click OK.

Tip: Start with a low parameter value, for example the default value, and increase
it until the best result is achieved. A useful strategy is to increase the parameter
value by the default value for each try.
Note: By default, smoothed curves are given the suffix SMTH. The default curve
name can be changed as needed.

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 How to edit results 11

11.2 How to subtract a blank run curve

Introduction Subtracting a blank run curve is a frequently used function in presentations,

especially if the curves have a drifting baseline or ghost peaks.

Ghost peaks If the ghost peaks come from impurities in the eluents, all equilibration of the
columns should be the same from method run to method run. If, for example, the
equilibration volume with buffer A is larger before a blank run curve than before
a separation, your ghost peaks might be higher in the blank run curve.

Example of a UV The illustration below shows the UV curve with baseline prior to subtraction of
curve with the baseline:
baseline

Example of a UV The illustration below shows the UV curve after subtraction of the baseline:
curve after subtrac-
tion of the
baseline

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 11 How to edit results
11.2 How to subtract a blank run curve

How to import a If a blank run curve was made, this might have been stored in another result file.
blank run curve If there is no blank run curve, you can create one with Integrate:Calculate baseline.
The table below describes how to import the blank run curve:

Step Action
1 Ensure that the destination chromatogram has been opened and is
the active window on the screen.

2 Choose File:Open:Curves.
Result: The Open Curves dialog box is displayed.

3 Double-click the result file that contains the blank run curve.
Result: The curves in the first chromatogram are displayed.

4 • Select the appropriate chromatogram in the Chromatogram list.

Result: The curves for that chromatogram are displayed on the

Available list.
• Select the curves that correspond to the blank run curve and click
the Select button.

Result: The selected curve is displayed on the Selected curves list.

5 • If you want to remove a curve from the list, select it and click the
Remove button.
• Click OK to import the curve.

Note: For more detailed information on how to import curves, chromatograms

and other results see 11.8 How to import and compare different runs on page 287.

How to subtract a You can subtract the blank run curve or the baseline from the sample curve. The
blank run curve table below describes how to do this:

Step Action
1 Select Operations:Subtract.
Result: The Subtract dialog box is displayed.

2 Select the sample chromatogram and curve in the left field and the
baseline or blank run curve to be subtracted in the middle field.
Click OK.

Note: All resulting curves from the subtract operation receive the SUB suffix by
default. The default curve name can be changed as needed.

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 How to edit results 11

11.3 How to add curves

Introduction In some method runs, several sequential chromatograms might have been created.
This can occur, for example, when the instruction New chromatogram has been used
in the method, thus creating different chromatograms during the run.
In order to view and evaluate the resultant curve of all the chromatogram parts,
the curves must be added together. Usually, you have a number of chromatograms
within the same result file and you want to add the curves. In some circumstances,
curves might need to be imported from other result files.

Instruction The table below describes how to add curves:

Step Action
1 Select and view the first chromatogram in the sequence.

2 Choose Operations:Add.
Result: The Add dialog box is displayed.

3 • Select the first curve in the desired sequence in the left field.
• Select the second curve in the sequence in the middle field.
• Click the OK button to add the two curves together in a new result
curve.

4 • Open the Add dialog box again.

• Select the result curve (.ADD) from the previous addition in the
left field.
• Select the next curve in the sequence in the middle field.
• Click OK to add the two curves together in a new result curve.

5 Repeat steps 3 and 4 until all curves have been added together. The
final curve should be the cumulative curve for the whole run.

Note: All curves created using the Add operation receive the ADD suffix by default.
The default curve name can be changed as needed. The original curves are
distinguished in the chromatogram by underlined curve names.

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11.4 How to enter and edit text in the chromatogram

11.4 How to enter and edit text in the chromatogram

How to enter text Text can be added to the chromatogram. The table below describes how to do this:

Step Action
1 • Right-click the curves view of the chromatogram window and
select Add text from the menu.

or
• Choose Edit:Text:Add.

2 • Click where you want to insert text in the chromatogram.

Result: A text box opens.

• Type the text.
• Click outside the text box to set the text.

How to edit the The table below describes how to edit inserted text:
text
Step Action
1 Choose Edit:Text:Edit.
Result: The Edit Texts tab of the Chromatogram Layout dialog box is
displayed.

2 • Select the text that you want to edit and make the appropriate
changes in the Selected text field.
• Click the Change text button or the Delete text button.
• Use the Font and Set Orientation buttons if needed, and make the
desired changes in the resulting dialog boxes.
• Click OK to apply the changes.

Shortcut option
You can also right-click outside the text box and select Edit Text Mode from the
shortcut menu. This activates all the text boxes in the chromatogram. The list
below describes how to edit the text:
• Click the text and type the new text.
• Click outside the text box to set the text.

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11.5 How to pool fractions

Introduction Fractions are collected sequentially during a separation. Each fraction contains a
set volume of sample. This section describes how to pool the information on several
fractions into a new curve.

How to view the Each fraction is numbered according to its order in the sequence. The information
contents of a frac- is saved as a curve under the name Fractions.
tion
• Select this curve on the Curve tab in the Chromatogram Layout dialog box to
display the contents of each fraction in relation to the information displayed
on the UV detection curve.

How to pool frac- The table below describes how to pool fractions.
tions
Step Action
1 • Choose Operations:Pool.

Result: UNICORN will automatically pool suitable fractions. The

pooled fractions are listed in a table below the chromatogram and
the pooled peaks are numbered sequentially in the chromatogram.

Note: Only adjacent fractions will be pooled. The fraction numbers

for each pool are listed in the table as a range in retention order, e.g.
A6-A7 etc.

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11.5 How to pool fractions

Step Action
2 The pooled fractions can be adjusted manually:
To include or exclude adjacent fractions in a pool
• Click the numbered marker under the pool and drag the sideline.

To add more pools

• Click between the droplines under a fraction to create a new pool,
and drag the sidelines to include more adjacent fractions.

To delete pools
• Click the numbered marker to select the pool and click the Delete
button. Click the Delete All button to clear all pools.

To restore the pools created by UNICORN

• Click the Default Pool button.

3 Other curves can be selected for the operation:

• Select another source curve from the Source curve droplist and
click the Default Pool button.

or
• Select another baseline curve from the Baseline droplist and click
the Default Pool button.

or
• Select another fraction curve from the Fraction curve droplist and
click the Default Pool button.

Result: The pooled fractions in the list are replaced by the pooled
fractions for the selected curve.

How to create a The pooled fractions can be stored as a new curve.

pool fraction
curve Note: You must store the pooled fractions as a new curve in order to be able to
proceed with other operations using the pooled fractions.

Step Action
1 • Choose Operations:Create Pool Fraction curve.

Result: The Create Pool Fraction Curve dialog box opens.

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Step Action
2 • Select a position where the curve will be stored from the Save
curve in list.
• If needed, type a new name in the Curve name text box.

Note: The suggested curve name will have the default suffix POOL.
• Click the OK button.

Result: The Pool Fraction curve is displayed in the chromatogram.

How to show only The active chromatogram will now show both the original and the pooled fraction
the pooled frac- curves. The table below describes how to show only the pooled fractions.
tions

Step Action
1 • Choose Edit:Chromatogram Layout.

or
• Right-click in the chromatogram and choose Properties from the
shortcut menu.

Result: The Chromatogram Layout dialog box opens.

2 • Select the Curve tab.

• De-select the check box for the original fraction curve (remove
the check mark).

Result: The original fraction curve is de-selected and is not displayed.

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11.5 How to pool fractions

How to calculate Protein concentrations

concentration and
amount in the The protein concentration in the fractions are calculated using the following
pools formula:
Concentration [mg/ml] = A / (d * 1000 *Ext.Coeff.)
A = Average fraction absorbance = Area / Volume [mAu].
d = UV-cell path length [cm]

Ext.Coeff. = Protein coefficient at used wavelength. [l g-1 cm-1]

Protein amounts
The total amount of protein found in the pool fraction is calculated using the
following formula:
Amount [mg] = Concentration [mg/ml] * pooled fraction volume [ml]
How to calculate the concentrations and amounts:
• Type the UV path length expressed in centimeters in the Path Length text box.
• Type the extiction coefficient in the Ext.Coeff. table cell for each pool.

Result: The sample concentration and amount for each pool is calculated in the
corresponding table cell.

How to determine The Target conc. and Target vol. cells are used to calculate the pool volume at a
a pool target specific concentration level. The result can then be used to determine if the pool
volume
needs to be concentrated further or diluted.
• Type the desired concentration level (mg/ml) in the Target conc. table cell.

Result: The corresponding target volume is calculated in the Target vol. table cell
using the following formula:
Target vol. = Conc. * (Vol./Target conc.)

How to use the A protocol of the pooled fractions can be printed for use when handling the samples.
Pooling Protocol The table below describes how to add pools to the Pooling Protocol and send the
list to a printer or export the list to a file.

Step Action
1 • Open a result file in the Evaluation module.
• Pool fractions as described in How to pool fractions above.
• Click the Add to Pooling Protocol button.

Result: The pooled fractions from the active result file is added to
the Pooling Protocol.

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Step Action
2 • Repeat step 1 to add pooled fractions from other result files.

Note: You will be asked to save the current file when you open the
next. The pool table will not be saved.

3 • Click the View Pooling Protocol button.

Result: The Pooling Protocol dialog box opens.

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11.5 How to pool fractions

Step Action
4 • Click the Show all fractions checkbox to display the individual
fractions instead of fraction ranges for the pools.
• Click the Show all columns checkbox to display all the information
columns from the Pool table.

Possible actions in the Pooling Protocol

To delete a single pool...
• select a pool and click the Delete button

To clear the whole protocol...

• click the Delete all button.

To print the protocol on the default Windows printer...

• click the Print button to print the protocol on the default Windows
printer.

To export the protocol...

• click the Export button to save the protocol in one of the following
formats:
- text (.txt)
- Excel (.xls)
- HTML (.htm)
- XML (.xml)

Note: The protocol is automatically saved for the user. The pooling
protocol will be available again when the user starts UNICORN the
next time.

5 • Click the Close button to close the Pooling Protocol dialog box.

Result: If the protocol was exported or only edited, the dialog box
will close. If the protocol was printed, a dialog box will open asking
if you want to delete the list and start a new.

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 How to edit results 11

11.6 How to match protein activity to a curve

Introduction You can compare data from the results of protein activity assays, such as ELISA,
with the data contained in the UV curve. The activity curve and the UV curve can
be compared in a combined presentation.

The Activity Histo- The illustration below shows the Activity Histogram dialog box:
gram dialog box

How to enter pro- The table below describes how to enter the values from a protein activity assay in
tein activity values a comparison histogram:
for comparison

Step Action
1 Choose Operations:Activity Histogram.
Result: The Activity Histogram dialog box opens.

2 By default, the fraction curve for the specific chromatogram is selec-

ted.
• If necessary, change the source and target chromatograms.

All the component fractions of the fraction curve are listed in the
Fraction/Activity field.
• Type an activity value for each fraction in the Activity column.
• Click OK.

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11.7 How to rename chromatograms, curves and peak tables

11.7 How to rename chromatograms, curves and peak

tables

Instruction The table below describes how to rename chromatograms, curves or peak tables
in the Evaluation module:

Step Action
1 Choose Edit:Rename and the relevant option Chromatogram, Curve or
Peak Table.
Result: The Rename dialog box opens.

2 • Select the appropriate object.

• Type a new name in the Name field.
• Click OK.

Note: The original raw data curves cannot be renamed. They will not be listed as
options in the dialog box.

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11.8 How to import and compare different runs

Introduction This section describes

• how to make comparisons between curves or chromatograms from different
runs
• how to present curves or chromatograms from different runs.
• how to compare curve parameters among curves from different runs
• how to view several chromatograms at the same time
• how to overlay curves from different runs in one chromatogram
• how to stack curves from different runs in one chromatogram
• how to stretch curves to make comparisons easier
• how to create mirror images

In this section This section contains these sub-sections:

Topic See
How to use the Multifile Peak Compare wizard 11.8.1

How to import and compare chromatograms 11.8.2

How to import and compare curves 11.8.3

How to stack and stretch curves 11.8.4

How to produce a mirror image 11.8.5

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11.8 How to import and compare different runs
11.8.1 How to use the Multifile Peak Compare wizard

11.8.1 How to use the Multifile Peak Compare wizard

Introduction This section describes how to use the Multifile Peak Compare wizard to make
comparisons between different results, for example, by comparing area, retention
etc. The difference can be presented graphically or in a spreadsheet.

Step 1: How to se- The table below describes how to select the operation:
lect the Operation
Step Action
1 In the Evaluation module,
• choose File:Multifile Peak Compare:Start Wizard

or
• click the Multifile Peak Compare toolbar icon:

Result: The Multifile Peak Compare wizard entry dialog box is dis-
played.

2 Click the Next button to display the Operation dialog box.

3 Select
• one of the available operations (see descriptions of the operations
below this table)
• a retention unit.

If you select Batch quantitate:

• Select a quantitation table in the Select quantitation table field.

If you select Batch Mw determination:

• Select a molecular size table in the Select mol. size table field.

Click the Next button to proceed to the Data Selection dialog box.

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The Operation The illustration below displays the Operation dialog box:
dialog box

The operation op- The table below is a brief description of the operation options:
tions
Operation Description
Compare peak data This option is used to compare differ-
ent results.

Compare scouting runs This option is used to compare the

results from scouting runs. The scout-
ing variables can be displayed.

Media life time study This option features different default

values than the Compare peak data
option, specially selected to measure
changes in the column media.

Batch quantitate This option is used to run several

quantitations. This is an alternative to
Quantitate:Calculate Amount and Conc.
which is used to quantitate single res-
ults. A quantitation table must be cre-
ated before this option can be used.
This option is available only if the
Analysis module has been installed.

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 11 How to edit results
11.8 How to import and compare different runs
11.8.1 How to use the Multifile Peak Compare wizard

Operation Description
Batch Mw determination This option is used to batch run mo-
lecular size calculations. This is an al-
ternative to Mol. Size:Calculate
Mol.Size, which is used for single cal-
culations. A molecular size table must
be created before this option can be
used.
This option is available only if the
Analysis module has been installed.

The Data Selec- The illustration below shows the Data Selection dialog box.
tion dialog box

Step 2: How to se- The table below describes how to select data to compare:
lect data to com-
pare
Step Action
1 • Use the drop-down lists and Browse buttons in the Chromatogram
selection field to specify the result files, chromatograms and curves
for comparison.
• Click the All button if you want to select all available results,
chromatograms or curves.

2 • Click the Search button in the Found curves field.

Result: A list of all curves that matched the search criteria is displayed
in the Found curves field.

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 How to edit results 11

Step Action
3 • Select the check boxes (or click the Select All button) of the desired
curves within the Found curves field.
• Click the Next button to proceed to the Peak Data Selection dialog
box.

4 • If all the chosen curves have been integrated, go to "Step 3: How

to select the peaks" in this section.
• If any of the chosen curves have not been integrated, the Curves
not Integrated dialog box is first displayed:

• If desired, change the default value for the peak number selection
filter.
• Click the Integrate button.

Result: The Peak Selection dialog box is displayed.

Note: If the results from the automatic peak integration is not satis-
factory you must cancel the wizard and perform the integration
manually. See 12.1.2 on page 330.

The Peak Selec- The illustration below displays the Peak Selection dialog box:
tion dialog box

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 11 How to edit results
11.8 How to import and compare different runs
11.8.1 How to use the Multifile Peak Compare wizard

Dialog box description

The dialog box displays the following properties for the first of the chosen curves:
• The integrated peak and the associated peak table
• The Peak identification settings table. Its purpose is to identify the peak parameter
to be used in the comparison.

How to adjust im- The table below describes what to do if the peaks in the curve window do not
proper peak integ- appear to be integrated properly (for example if ghost peaks are labelled).
rations

Step Action
1 Click the Cancel button to quit the wizard.

2 Perform a peak integration (see 12.1.2 How to perform a peak integ-

ration on page 330) and verify that the resulting curve is properly
integrated.

3 Repeat the Multifile Peak Compare wizard operation.

Step 3: How to se- The table below describes how select peaks in the Peak Selection dialog box:
lect the peaks
Step Action
1 Choose a curve in the curve window:
• Double-click the peak, or click the peak once and then click the
Select peak button.

Result: The peak is assigned a letter (A, B, C....) and the peak para-
meters are displayed in the Peak identification settings table.

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Step Action
2 Set the desired peak identification criterion:
• Click the desired parameter value in the Peak identification settings
table.

Example: If you have selected the highest peak in the curve and want
to compare the highest peak among all curves, select the Height check
box.
In the illustration below, the initial (A) peak and the Height check
box have been selected:

3 If desired, you can assign a name to a chosen peak:

• Click the name of the row, for example A.
• Click the Set peak name button.
• Type a new name and click OK.

Note: This can be useful when you compare multiple peak parameters
and you wish to have peak names other than “Peak A”, “Peak B”,
etc. to simplify peak identification and clarity f.ex. when comparing
peak data between batch quantitated results.

4 Repeat steps 1-3 for other desired peaks in the current curve.

5 Use the Next curve and Previous curve buttons to navigate forward
and backward among your selected curves and manually check the
selections made by the software if necessary.

6 Other possible actions you can perform

• If the current curve does not prove useful for your comparison,
click the Delete curve button to delete it from the comparison.
• Click the Back button to navigate back to the Data Selection dialog
box and add new curves to your comparison.

See also How to change the peak identification below.

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 11 How to edit results
11.8 How to import and compare different runs
11.8.1 How to use the Multifile Peak Compare wizard

Step Action
7 When all peak selections and identification settings are complete,
click the Next button to proceed to the Peak Data Selection dialog
box.

Note: Click and drag in the curve window to zoom into selected peaks to simplify
accurate peak identification. Right-click and click the Reset Zoom button to reset
the zoom to the full view.

How to change In the Peak identification settings table, each column identifies a peak parameter
the peak identifica- to be compared among all peaks. If UNICORN has identified other peaks than
tion
the intended ones, you can change the peak identification manually. The table
below describes how to change the identification:

If you want to... then...

remove a peak identific- • click the desired peak in the curves window
ation • click the Set as peak button
• choose None in the Set As Peak dialog box
• click OK.

replace or add a peak • click a peak in the curves window

identification • click the Set as peak button
• choose a letter in the Set As Peak dialog box
• click OK.

remove a row from the • select the row

table • click the Delete row button.

Note: If you click Delete row without first selecting a

row, the first row (A) is deleted by default.

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Step 4: How to se- The illustration below displays the Peak Data Selection dialog box:
lect the Peak Data

The table below describes how to select the peak data:

Step Action
1 • In the Select Peak Data list, select the peak characteristics on the
list that you want to include in your comparisons.
• If available, select the appropriate Scouting variables.

2 • Click the Next button and proceed to step 5, How to use the Data
View dialog box below.

Note: If Media life time study was chosen in the Operation dialog box
when the wizard was started, 2D Plot is selected in the Data View
dialog box.

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11.8 How to import and compare different runs
11.8.1 How to use the Multifile Peak Compare wizard

Step 5: How to The Data View dialog box presents a comparison of the chosen data for the
use the Data View designated peak comparisons. The illustration below shows the dialog box:
dialog box

The table below describes how to use the command buttons of the dialog box:

Command button Function

2D Plot Displays the data in 2-dimensional plot. See "How
to use the 2D Data View dialog box" below.

3D Plot Displays the data in 3-dimensional plot. See "How

to use the 3D Data View dialog box" below..

Print Prints the spreadsheet.

Save Spreadsheet Allows you to save the data in different formats:

• Excel (.xls)
• Tabbed text (.txt)
• FarPoint spread (.ss3)

Save Wizard Settings See "How to save the Wizard Settings" below.

Cancel Ends the Multifile Peak Compare wizard.

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 How to edit results 11

How to use the The 2D Data View dialog box presents a two-dimensional plot of a selected peak.
2D Data View See also "How to use the 2D Data View shortcut menu" below. The illustration
below shows the dialog box:

The list boxes

Use the list boxes to select which peak to plot and the units of the x- and y-axes.
The command buttons
The table below describes how to use the command buttons of the dialog box:

Command button Description

Spread Returns to the Data View dialog box.

3D Plot Displays the data in 3-dimensional plot. See "How

to use the 3D Data View dialog box" below.

Print Prints the spreadsheet.

Copy to Clipboard Stores a figure for transfer to an external program.

Save Wizard Settings See "How to save the Wizard Settings" below.

Cancel Ends the Multifile Peak Compare wizard.

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11.8 How to import and compare different runs
11.8.1 How to use the Multifile Peak Compare wizard

How to use the Click the right mouse button in the plot area of the 2D Data View dialog box to
2D Data View open the shortcut menu. See illustration below:
shortcut menu

A wide array of plot presentation options can be found on the shortcut menu. Two
of them are described below:
• Select Customization Dialog to open a dialog box which allows further
customization of the graph:

• Select Export Dialog to export the view.

Note: You can also click the Export button from the Customization dialog box.

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 How to edit results 11

How to use the The 3D Data View dialog box presents a three-dimensional plot of a selected peak.
3D Data View See also "How to use the 3D Data View shortcut menu" below. The illustration
dialog box
below shows the dialog box:

The list boxes

Use the list boxes to select which peak to plot and the units of the x-, y- and z-axes.
The command buttons
The table below describes how to use the command buttons of the dialog box:

Command button Function

Spread Returns to the Data View dialog box.

2D Plot Displays the data in 2-dimensional plot. See "How

to use the 2D Data View dialog box" above.

Print Prints the spreadsheet.

Copy to Clipboard Stores a figure for transfer to an external program.

Save Wizard Settings See "How to save the Wizard Settings" below.

Cancel Ends the Multifile Peak Compare wizard.

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11.8 How to import and compare different runs
11.8.1 How to use the Multifile Peak Compare wizard

How to use the Click the right mouse button in the plot area of the 3D Data View dialog box to
3D Data View open the shortcut menu. See illustration below:
shortcut menu

The 3D Data View shortcut menu differs some from the 2D Data View shortcut menu
and allows the figure to be viewed by animated rotation. The shortcut menu displays
different plot presentation options.
• Select Customization Dialog to open a dialog box that allows further customization
of the graph:

• Select Export Dialog to export the view.

Note: You can also click the Export button from the Customization dialog box.

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 How to edit results 11

How to save the The wizard settings can be saved from either of these dialog boxes:
Wizard Settings
• The Data View dialog box
• The 2D Data View dialog box
• The 3D Data View dialog box

The table below describes how to save the wizard settings:

Step Action
1 • Click the Save Wizard Settings button.

Result: The Save Wizard Settings dialog box opens.

2 Type a name in the Wizard settings name field.

3 • If the settings are to be used by all users on the system, select the
Global wizard settings check box.
• Click OK.
• Click Cancel to close the wizard.

Note: The Global wizard settings check box can also be used to toggle
between lists of stored global and stored user settings.

How to open the The table below describes how to open the saved wizard settings:
saved wizard set-
tings
Step Action
1 • Choose the File:Multifile Peak Compare:Start Wizard With Settings
menu item.

Result: The Select Wizard Settings dialog box opens.

2 • Select the desired saved settings from the list.

• Click OK.

Result: The Multifile Peak Compare wizard opens with the saved
settings.
Note: The Global wizard settings check box is used to toggle between
lists of stored global and stored user settings.

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11.8 How to import and compare different runs
11.8.2 How to import and compare chromatograms

11.8.2 How to import and compare chromatograms

Introduction This section describes

• how to import chromatograms from other result files,
• how to compare with chromatograms in an already opened result file.

Commands to use Two commands in the Evaluation module can be used to import chromatograms
from result files into an already opened result file:
• File:Open to compare
This is the preferred option when you search for many chromatograms in a
specific folder based on defined selection criteria. See “How to import
chromatograms with the command File:Open to compare” below.
• File:Open
This is the preferred option to import any individual chromatograms from result
files in different folders. See “How to import chromatograms with the command
File:Open” below.

How to import The table below describes how to import chromatograms with the File:Open to
chromatograms compare command. The search is performed at specific locations or with specific
with the command
File:Open to com- search criteria. This method is useful if you, for example, want to import
pare chromatograms from all files of a scouting folder.

Step Action
1 Choose File:Open to compare:Chromatograms in the Evaluation module.
Result: The Open Chromatogram to Compare dialog box is displayed.

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Step Action
2 • Click the Search button in the Found chromatograms field and a
list of chromatograms will be displayed based on the designated
search criteria.
• A new search can be performed with new search criteria without
erasing the first found chromatograms from the list.
• Select the chromatograms that you want to import. If you click
the Select All button, all the displayed chromatograms will be
imported.
• If you want to clear the list of displayed chromatograms, click
the Clear button.
• Click OK.

Result: All the selected chromatograms are shown in the Evaluation

workspace.
Note: If the names of the imported chromatograms already are used
they will be sequentially numbered for identification purposes. Up
to 10 chromatograms can be made available at the same time in the
Evaluation workspace.

How to import The table below describes how to import chromatograms one by one, using the
chromatograms command File:Open:
with the command
File:Open
Step Action
1 Choose File:Open:Chromatogram in the Evaluation module.
Result: The Open Chromatograms dialog box is displayed.

2 Double-click a result file to select it.

Result: All the chromatograms contained in the result file will be
displayed in the Available field.

3 • Select the chromatogram(s) of interest and click the Select button.

Result: Selected chromatograms are added to the Selected chromato-

grams list.
Note: Chromatograms can be deselected with the Remove button.

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11.8 How to import and compare different runs
11.8.2 How to import and compare chromatograms

Step Action
4 • Repeat steps 2-3 if you want to import chromatograms from
other result files.
• Click OK.

Note: If the names of the imported chromatograms already are used

they will be sequentially numbered for identification purposes. Up
to 10 chromatograms can be made available at the same time in the
Evaluation workspace.

How to display The table below describes how to simultaneously display and compare the imported
and compare the chromatograms:
imported chroma-
tograms
Step Action
1 In the Evaluation module, select
• Window:Tile to display the chromatograms side by side.

or
• Window:Cascade to display the chromatograms in layers.

Note: Chromatogram windows can be individually sized and the

presentation of the curves changed.

2 Display all chromatograms on the same scale

• Open the Chromatogram Layout dialog box for any chromatogram
• Make the changes to the chromatogram axes.
• Select the Apply to all chromatograms option.

Note: Imported chromatograms cannot be shown with column volume as the

X-axis base.

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11.8.3 How to import and compare curves

Introduction This section describes how to import or copy curves from different runs into one
chromatogram for comparison.

Commands to use Two commands can be used to import curves from result files into one
chromatogram:
• File:Open to compare
This is the preferred option if you want to automatically search result files that
are stored in the same folder to locate all curves of a specified type, for example,
all UV curves. This is especially useful for comparison of curves from scouting
runs. Moreover, the imported curves can be automatically overlaid, stacked or
presented as mirror images. See "How to use File:Open to compare" below.
• File:Open:Curves
This is the preferred option to import individual curves. See " How to use
File:Open:Curves" below.

Note: Original curves are underlined in the chromatogram, imported and created
curves are not underlined.

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11.8 How to import and compare different runs
11.8.3 How to import and compare curves

How to use The table below describes how to import curves to a chromatogram with the
File:Open to com- command File:Open to compare:
pare

Step Action
1 In the Evaluation module,
• choose File:Open to compare:Curves

or
• click the Open curves to compare toolbar button.

Result: The Open Curves to Compare dialog box opens.

2 • Select the desired search criteria in the Folder, Result, Chromatogram

and Curve name droplists of the Chromatogram selection section.
• Click Search and a list of found curves will be displayed based
on the selected search criteria.

Note: A new search can be performed with new search criteria

without erasing curves located in the previous search.
• Select the check boxes for the curves that you want to import.
Click the Select All button if you want to import all the curves.
• If you select the Store in new chromatogram option, the curves will
be imported into a new chromatogram. This is recommended to
keep the source chromatogram free of too many additional curves.

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 How to edit results 11

Step Action
3 Select how to display the imported curves in the Curve options field
and click OK. See the options below:
Overlay

The curves are presented overlaid on one another.

Stack

The curves are presented with a given offset Y-axis value so that the
curves are stacked and distinct from one another.
Mirror

For comparison of two imported curves. One curve is inverted in

the Y-axis and thus appears to mirror the other curve.

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 11 How to edit results
11.8 How to import and compare different runs
11.8.3 How to import and compare curves

Step Action
4 If you selected the Stack option in step 3, the Shift Curves by Offset
dialog box is displayed:

• You can set the Offset value to increase or decrease the offset
distance between the curves.
• Click OK.

Result: Depending on your previous choices, the imported curves

are now displayed in the source chromatogram or in a newly created
chromatogram.
Note: If curves with several different units have been selected, the
curves with each different unit will be grouped together with separate
offset from the other groups.

5 Change some comparison settings

• Choose Edit:Chromatogram Layout to open the Chromatogram Layout
dialog box.
• Select or de-select the check boxes on the Curve tab to compare
a different set of curves.
• On the Y-Axis tab, the curves can be scaled
- individually
- all with the same scale (click the All with this unit button).
• Click OK to display the curves.

6 If you stacked the curves and want to change the stack offset
• choose Operations:Shift offset
• type a new Offset value and click OK.

Note: The individual curves can also be moved (see 11.8.4 How to
stack and stretch curves on page 312).

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 How to edit results 11

How to use The table below describes how to import individual curves into an active
File:Open:Curves chromatogram with the File:Open:Curves command:

Step Action
1 Make sure that the destination chromatogram for the imported
curve(s) is active on the screen.
• Select File:Open:Curves in the Evaluation module.
Result: The Open Curves dialog box is displayed.

2 Select curves in the Open curves dialog box

• Select the folder and the result file in the upper part of the dialog
box.
• Select a chromatogram on the Chromatogram drop-down list.
Usually there is just one chromatogram.

Result: The available curves are listed on the Available list.

• Click the check boxes on the Available list for the curves that you
want to import and click the Select button.

Result: The selected curve(s) is displayed in the Selected curves list.

To remove a curve from the Selected curves list, click the check box
and then click the Remove button.

3 • Repeat step 2 if you want to import curves from other chromato-

grams.
• Click OK when you have selected the curves you want.

4 Change some comparison settings

• Choose Edit:Chromatogram Layout to open the Chromatogram Layout
dialog box.
• Select or de-select the check boxes on the Curve tab to compare
a different set of curves.
• On the Y-Axis tab, the curves can be scaled
- individually
- all with the same scale (click the All with this unit button).
• Click OK to display the curves.

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 11 How to edit results
11.8 How to import and compare different runs
11.8.3 How to import and compare curves

How to copy A practical way to compare curves is to create a chromatogram and copy curves
curves into one from different chromatograms into the new chromatogram. The comparisons are
chromatogram
then performed in the new chromatogram.
The table below describes how to copy curves into a chromatogram:

Step Action
1 Perform either A or B below:
A. Create a new chromatogram
• Choose File:New:Chromatogram to create a new chromatogram.

B. Use the Temporary chromatogram

• Choose Window:Temporary.

2 Open the source chromatogram(s)

Choose File:Open:Chromatogram to open the chromatogram(s) that
contains the curves you want to copy.
Result: The Open Chromatogram dialog box opens.

3 • Select the result file.

• Click the check box for the source chromatogram in the Available
list.
• Click the Select button.
• Click OK.

Result: The source chromatogram opens.

4 Copy the curves

• Choose Edit:Copy:Curves.

Result: The Copy Curve dialog box is displayed.

5 • Select the source chromatogram and a curve of interest in the

Source Chromatogram field.
• Select the target chromatogram (the one you created, or Tempor-
ary) in the Target Chromatogram field.
• Click the Copy button.
• Repeat this step for as many curves as you want, from the same
or other chromatograms.
Note: You can open more source chromatograms with the
File:Open:Chromatogram command.
• Click the Close button when you have copied all curves.

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 How to edit results 11

Step Action
6 Change some comparison settings
• Make sure the target chromatogram is open and that its window
is active.
• Choose Edit:Chromatogram Layout to display the Chromatogram
Layout dialog box.
• Select the curves that you want to view on the Curve tab and click
OK.
• The curves can be scaled individually or all with the same Y-axis
scale. Use the All with this unit button on the Y-Axis tab to scale
all curves with the same scale.

7 If you used the Temporary chromatogram

• If you used the Temporary chromatogram you can perform evalu-
ations in the Temporary chromatogram and transfer the final
curves to other destination chromatograms.
• All of the contents in the Temporary chromatogram can be re-
moved with Edit:Clear Temporary Chromatogram.

Alternative way to copy curves

An alternative way to copy curves into one chromatogram is to
• create a new chromatogram by copying an existing chromatogram and saving
it under a new name
• import more curves into the new chromatogram according to the instructions
described above in this section.

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 11 How to edit results
11.8 How to import and compare different runs
11.8.4 How to stack and stretch curves

11.8.4 How to stack and stretch curves

Functions You can stack and stretch curves from different runs to better visualize the
differences. To achieve this you can use the following functions:
• Normalise
• Shift
• Multiply.

Note: All the functions require the curves to be present in one chromatogram.

How to use the The Normalise function provides the simplest method to align curves with respect
Normalise func- to the X-axis or the Y-axis for easier visualization.
tion
The table below describes how to use the Normalise function:

Step Action
1 • Make sure that a chromatogram with the relevant curves is open
in the Evaluation module.
• Choose Operations:Normalise.

Result: The Normalise dialog box is displayed.

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 How to edit results 11

Step Action
2 • Select the curve you want to normalise in the left (Select curve to
normalise) field.
• Select the reference curve you want to normalise against in the
right (Select help curve) field.Example: If you want to stack the
curves, select the curve at the bottom of the stack as the reference
curve.
• Click OK.

Result: The Normalise window is displayed, where a box surrounds

the curve selected to be normalised.

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 11 How to edit results
11.8 How to import and compare different runs
11.8.4 How to stack and stretch curves

Step Action
3 In the Normalise window, you can use the following command but-
tons:
Size Allows the arrow keys to be used to stretch the selected curve
along its Y-axis or X-axis. This is useful for comparison of curves
with, for example, different gradient lengths.
• Click the Size button and use the arrow keys to stretch the the
curve either along its Y-axis or X-axis.

Move Allows the arrow keys to be used to move the selected curve
to any position on the chromatogram. Axes are automatically re-
scaled to accommodate the new positioning. This function is useful
for stacking curves.
• Click the Move button and use the arrow keys to move the curve
into position. The curve can also be moved with the mouse
pointer. Click the mouse button when the curve is in the correct
position.

Note: The curve can also be moved and sized with the mouse
pointer.
Normalise The curve to be normalised will be adjusted to the help
curve. Thus, the height of the highest peak on both curves will be
the same and will occur at the same retention point.
• Click the Normalise button. The curve to be normalised is auto-
matically moved along the X-axis and stretched along the Y-axis.
• Click OK to save the new normalised curve.

Result: The Save Curve dialog box opens.

4 Choose a curve position to save the curve in and click OK.

5 • Choose Edit:Chromatogram Layout to open the Chromatogram Layout

dialog box.
• Select the normalised curve for viewing on the Curve tab.
• Click OK.

6 Repeat steps 1-5 for all curves you want to stack or stretch.

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 How to edit results 11

How to move a If you want to position a curve more precisely, the Shift function should be used.
curve with the The function is similar to Normalise:Move but each curve is repositioned by a precise
Shift function
value instead of by eye and the instruction is logged in the evaluation log. The table
below describes how to use the Shift function:

Step Action
1 • Make sure that a chromatogram with the relevant curves is open
in the Evaluation module.
• Choose Operations:Shift.

Result: The Shift dialog box is displayed.

2 • Select the curve to be shifted in the Source chromatogram list.

• Select a curve position in the Target chromatogram list.
• Type a new Curve name or accept the default.
• Select the axis/axes along which the shift is to be made:
- along the X-axis (Shift retention)
- along the Y-axis (Shift amplitude).
• Type the shift value(s).
• Click OK.

How to stretch Curves can be stretched or shrunk on the x or y plane with the Multiply function.
and shrink a curve This function is similar to Normalise:Size, but each curve is repositioned with precise
with the Multiply
function numbers instead of by eye and the instruction logged in the evaluation log. The
table below describes how to use the Multiply function:

Step Action
1 • Make sure that a chromatogram with the relevant curves is open
in the Evaluation module.
• Choose Operations:Multiply.

Result: The Multiply dialog box is displayed.

2 • Select the curve to be multiplied in the Source chromatogram list.

• Select a curve position in the Target chromatogram list.
• Type a new Curve name or accept the default.
• Select the axis/axes along which the multiplication is to be made:
- along the X-axis (Multiply retention)
- along the Y-axis (Multiply amplitude).
• Type the multiply value(s).
• Click OK.

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 11 How to edit results
11.8 How to import and compare different runs
11.8.5 How to produce a mirror image

11.8.5 How to produce a mirror image

Instruction A very useful way to compare the features of two curves is to produce a mirror
image of one curve. The table below describes how to do this:

Step Action
1 • Make sure that a chromatogram with the relevant curves is open
in the Evaluation module.
• Choose Operations:Multiply.

Result: The Multiply dialog box is displayed.

2 • Select the curve to be multiplied in the Source chromatogram list.

• Select a curve position in the Target chromatogram list.
• Type a new Curve name or accept the default.
• Select the Multiply amplitude check box.
• Type the multiply value -1.
• Click OK.

Result: The mirror image of the original curve is displayed in the

active chromatogram window.

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 How to edit results 11

Step Action
3 Shift the mirror image curve downwards
Shift the mirror image curve downwards for an improved presenta-
tion:
• Choose Operations:Shift.
Result: The Shift dialog box is displayed.
• Select the curve to be shifted in the Source chromatogram list.
• Select the same curve number in the Target chromatogram list box
as in step 2.
• Select the Shift amplitude check box since the shift is to be made
along the Y-axis.
• Type a shift value.
• Click OK.

The illustration below shows the original curve and the mirror image
displayed.

4 If you want to display other curves in the active chromatogram

window,
• choose Edit:Chromatogram Layout to open the Chromatogram Layout
dialog box
• select the curves that you want to display
• click OK.

• p 317
 11 How to edit results
11.9 How to import and export results

11.9 How to import and export results

Introduction Curves and data can be imported and exported in different formats. This section
describes how to import and export results.

In this section This section contains these topics:

Topic See
How to import results 11.9.1

How to export results 11.9.2

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 How to edit results 11

11.9.1 How to import results

Introduction This section describes how to import curves in different formats and how to import
result data from SMART Manager, FPLCdirector™ or ÄKTAprime™.

Curve formats You can import curve files in the following formats:
• AIA (.cdf)
• ASCII (text)
• Lotus 1-2-3 spreadsheet (.wks)

How to import The table below describes how to import curves.

curves
Step Action
1 Choose File:Import:Curve.
Result: A menu with the available curve formats opens.

2 Choose the correct curve format.

Result: The Choose File to Import From dialog box opens.

3 Locate the file that contains the curve and double-click the file.
Result: The Import Curves dialog box opens.

4 • Select the curve(s) to import and click the OK button.

Result: The curves are opened in the Evaluation module.

How to import The table below describes how to import data from SMART Manager and
data from FPLCdirector:
SMART Manager
and FPLCdirector
Step Action
1 Choose File:Import:Result.
Result: A menu box with the available data sources opens. This box
opens immediately after Import if no result file is open in the Evalu-
ation module.

2 Choose FPLCdirector or SMART.

Result: The Import FPLCdirector Result dialog box or the Import SMART
Result dialog box opens.

3 Locate and double-click the result file.

Result: The result file is opened in the Evaluation module.

• p 319
 11 How to edit results
11.9 How to import and export results
11.9.1 How to import results

Copy from a floppy disk

When you import SMART or FPLCdirector files from a floppy disk it is best to first
copy the files to the hard disk and then import the files.

How to import The table below describes how to import ÄKTAprime data in the Evaluation module.
data from ÄK-
TAprime
Step Action
1 Choose File:Import:Result.
Result: A menu box with the available data sources opens. This box
opens immediately after Import if no result file is open in the Evalu-
ation module.

2 Choose ÄKTAprime.
Result: The ÄKTAprime Data Collection wizard opens.

3 • Click the Next button and follow the instructions to connect ÄK-
TAprime to the correct serial port on your computer.
• Click the Finish button to complete the setup.

Result: All open result files are closed and UNICORN is set up to
receive sample data from ÄKTAprime.

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 How to edit results 11

11.9.2 How to export results

Introduction This section describes how to export curves in different formats and how to copy
data and curves to the clipboard.

Data formats You can export data in the following formats:

• AIA (.cdf)
• ASCII (.asc)
• Lotus 1-2-3 (.wks)
• Excel (.xls)
• XML (.xml)

Export options Select File:Export in the Evaluation module to export data from an open result file.
The following export options are available:
• Curves
• Export curve to AIA
• Peak table
• Method
• Documentation
• Evaluation log

How to export The table below describes how to export curves in the Evaluation module.
curves
Step Action
1 Choose File:Export:Curves.
Result: The Export Curves dialog box opens.

• p 321
 11 How to edit results
11.9 How to import and export results
11.9.2 How to export results

Step Action
2 • Select the curve(s) you want to export.
• Enter parameters to limit the curve(s) if necessary.
• Click the Select button.
• Repeat Step 2 to select more curves.

3 Click the Export button.

Result: The Export Curves to File dialog box opens.

4 Select the export file format from the Save as type droplist.
• ASCII files (*.asc)
• Lotus 1-2-3 files (*.wks)
• Excel files (*.xls)
• AIA files (*.cdf)

5 • Select a destination folder.

• Type a file name and click OK.

Note: Curves are exported as series of numerical coordinates that refers to the
time/volume and signal respectively.

How to limit the You can optimize the exported curves to only the parts that you want to focus on,
exported curves in the Export Curves dialog box. The table below describes how to use these editing
options.

Dialog box option Instruction

Cut curves Enter retention values in the text boxes
to limit the curve to only a portion of
the original curve.

Cut graphically This button opens the Export Cut dialog

box. Move the vertical markers to the
correct cutoff points.

Reduce number of samples Adjust the factor value or the maxim-

um number of samples. To reduce the
number of samples by a factor of five
means that only every fifth point will
be sampled for export.

Normalise retention Select the Normalise retention checkbox

to have all exported curves normalized
to a common X-axis.

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 How to edit results 11

How to export The table below describes how to export curves in AIA format.
curves in AIA
format
Step Action
1 Select File:Export:Export curve to AIA.
Result: The Export curve in AIA format dialog box opens.

2 • Select the source chromatogram and the curve you want to export.
• Click the Export button.

Result: The Export Curves to File dialog box opens.

3 • Select a destination folder.

• Type a file name.
• Click OK.

How to export The table below describes how to export peak tables.
peak tables
Step Action
1 Choose File:Export:Peak Table.
Result: The Export Peak Table dialog box opens.

2 • Select the source chromatogram and the peak table you want to
export.
• Click the Export button.

Result: The Export Peak Table to File dialog box opens.

3 Select the export file format from the Save as type drop-list.
• ASCII files (*.asc)
• Lotus 1-2-3 files (*.wks)
• Excel files (*.xls)
• XML files (*.xml)

4 • Select a destination folder.

• Type a file name.
• Click OK.

Note: Peak tables are exported as text strings in ASCII format and numerical values
in the Lotus 1-2-3 formats. All possible columns in the peak table are exported.

• p 323
 11 How to edit results
11.9 How to import and export results
11.9.2 How to export results

How to export The table below shows how to export methods, documentation and evaluation
methods, docu- logs:
mentation and
evaluation logs
Step Action
1 Select the data you want to export.

2 • Select options in the dialog box.

• Click the Export button.

3 • Select a destination folder and type a file name.

• Click OK.

Copy to the clip- You can also use the Windows clipboard to copy the contents of the active window
board and paste it into other programs, e.g. Microsoft Word. Curves and documentation
are copied as Windows enhanced metafiles (.emf) and peak tables are copied as
text. Only the peak table columns that are selected in the spreadsheet will be copied.

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 How to edit results 11

11.10 How to sign results electronically

Instruction Result files can be signed electronically to enhance data file security. The table
below describes how to sign a result file electronically in the Evaluation module:

Step Action
1 Choose File:Sign Result.
Result: The Sign the Result dialog box opens.

2 • The Sign as user field shows the properties for the current user.
You can also choose another user from the droplist. If you choose
a new user, the corresponding password must be typed in the
Password text box.
• Type a short text description for the signed operation in the
Meaning field (e.g. Peak integration performed).
• The Lock check box is selected as default, to lock the result file
from further changes.
• Type your signature password in the Password field and click OK.

Note: You should only lock the result when you are sure that the
result file will not be modified anymore.

Signatures associ- The View Signatures tab of the Sign the Result dialog box provides a list of all
ated with the res- signatures associated with the current result. The information on this tab is for
ult
viewing purposes only and cannot be changed.

• p 325
 11 How to edit results
11.11 How to save results and exit the Evaluation module

11.11 How to save results and exit the Evaluation module

Introduction After you have finished the evaluation process, you can save all the changes you
have made to the chromatograms, including newly created curves and
chromatograms that you have imported and created.

How to delete un- All the curves that you created during your manipulations will be saved in the
wanted curves chromatogram. If some of these curves are not be needed anymore, select
Edit:Delete:Curves in the Evaluation module to remove the curves.
Note: The original curves that were created during the run can never be deleted.

How to save the You can either save your edited results in the original file or in a new result file.
results The table below describes how to save the results in the Evaluation module.

If you want to save the then...

edited results...
in the original result • select File:Save.
file
or
• click the Save toolbar icon.

in a new result file • select File:Save as.

Note: The previous version of the result file will be overwritten if you save the
changes. This cannot be reversed. However, the raw data curves remain unchanged.

How to exit the The table below describes how to exit the Evaluation module:
Evaluation mod-
ule
Step Action
1 Choose File:Exit.
Result: If there are unsaved changes, a dialog box opens with an
option to save the changes before exit.

2 Select Yes if you want to save the changes.

Result: The result file is closed in the Evaluation module and the
UNICORN Manager module is displayed.

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 Evaluation 12

12 Evaluation

Introduction This chapter describes:

• How to evaluate results with the focus on how to integrate peaks.
• How to automate evaluation operations.
• How to export data and curves.

In this chapter This chapter contains these sections.

Topic See
Peak integration 12.1

Other evaluations 12.2

Automated evaluation procedures 12.3

• p 327
 12 Evaluation
12.1 Peak integration

12.1 Peak integration

Introduction Peak integration is used to identify and measure a number of curve characteristics
including peak areas, retention time and peak widths. This section describes:
• How to perform peak integrations.
• How to optimize peak integrations.

In this section This section contains these sub-sections:

Topic See
Baseline calculation 12.1.1

How to perform a peak integration 12.1.2

How to optimize the baseline with a morphological algorithm 12.1.3

How to optimize the baseline with a classic algorithm 12.1.4

How to edit the baseline manually 12.1.5

How to edit the peaks 12.1.6

How to integrate part of a curve and how to exclude or skim peaks 12.1.7

Measurements 12.1.8

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 Evaluation 12

12.1.1 Baseline calculation

Introduction The first step when you integrate peaks is to calculate a baseline. A correct baseline
is crucial for accurate calculation of the peak areas. This section describes the
options for how to calculate baselines in the Integrate dialog box.

Baseline options UNICORN offers several options for how to create an accurate baseline:
• To use the automatic Calculate baseline function.
• To create a baseline based on a blank curve.
• To use a Zero baseline.
• To reuse an existing baseline.

The Calculate The Calculate baseline instruction provides automatic calculation of the baseline.
baseline function In most cases the measurement is very accurate. The calculation can be performed
using the Morphological algorithm or the Classical algorithm.

Baselines based on A blank curve can be used as the baseline for peak integration.
a blank curve
• You can use a blank curve with the same chromatographic conditions as the
corresponding sample.

or
• You can subtract the blank run from the source curve and then perform peak
integration on the resulting curve with the Calculate baseline instruction.

Note: In addition to blank run curves, it is also possible to select any curve from
the current chromatogram as the baseline, e.g. an edited baseline.

Zero baseline To use a Zero baseline means that there is no baseline subtraction at all.

Reuse an existing To reuse an existing baseline for the selected curve is the default alternative
baseline whenever there is an existing baseline available. The option Correlated baseline is
selected if this is the case.

• p 329
 12 Evaluation
12.1 Peak integration
12.1.2 How to perform a peak integration

12.1.2 How to perform a peak integration

How to perform a The table below describes how to perform a basic peak integration.
peak integration
Step Action
1 Open a result file in the Evaluation module.

2 • Choose Integrate:Peak Integrate.

or
• Click the Peak Integrate toolbar icon.

Result: The Integrate dialog box opens.

3 • Select a source curve.

• Select a baseline or a calculation method from the Baseline list.

• Click OK to integrate with the default selections.

or
• Proceed with steps 4 to 6 to change the default selections.

Note: See also 12.1.3 How to optimize the baseline with a morpho-
logical algorithm on page 336 and 12.1.4 How to optimize the
baseline with a classic algorithm on page 340.

4 • Click the Baseline settings button to change the calculation al-

gorithm in the Settings dialog box. The default algorithm is Mor-
phological.
• Change the selections or values.
• Click OK

5 • Click the Peak window button to edit the peak window limits if
necessary.
• Click the Reject peaks button to set the parameters for peak rejec-
tion if necessary.
• Edit the Column height or Column V values if necessary.

03-0014-90 • p 330
 Evaluation 12

Step Action
6 • Click OK to integrate and close the dialog box.

or
• Click Save and Edit Peak Table to save the integration and open
the integrated curve for editing.
- See 12.1.5 How to edit the baseline manually on page 348
- See 12.1.6 How to edit the peaks on page 351
- See 12.1.7 How to integrate part of a curve and how to exclude
or skim peaks on page 358

Illustration This is an illustration of the Integrate dialog box:

• p 331
 12 Evaluation
12.1 Peak integration
12.1.2 How to perform a peak integration

Peak integration The peak table is displayed underneath the active chromatogram. The start point
results and end point of each peak are marked by vertical marks, drop-lines, in the
chromatogram. The peaks are automatically labelled according to what is selected
in the Curve Style and Color tab of the Chromatogram Layout dialog box.
This is an illustration of the results after a peak integration:

Note: Peak tables can be copied from one chromatogram to another with the
Edit:Copy command. However, to display the table you must right-click in the
chromatogram, choose Properties and then select the new peak table on the Peak
Table tab of the Chromatogram Layout dialog box.

How to display The peak retention times and several other peak characteristics are calculated
peak characterist- automatically. The table below describes how to display other peak characteristics.
ics

Step Action
1 • Right-click in the active chromatogram.
• Select Properties from the shortcut menu.

Result: The Chromatogram Layout dialog box opens.

2 Click the Peak Table tab.

3 • Select options from the Select peak table columns list.

• Click OK.

Result: The selected items will be displayed in the peak table.

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 Evaluation 12

How to filter Peaks can be removed from display in a peak table. The table below describes how
peaks from view to filter the peaks:

Step Action
1 • Right-click in the active chromatogram or peak table.
• Select Properties from the shortcut menu.

Result: The Chromatogram Layout dialog box opens.

2 Click the Peak Table tab.

3 • Click the check boxes in the Filter Peaks field to select the filter
criteria.
• Specify filter values.
• Click OK.

To filter peaks vs. The table below describes the major differences in the effect of filtering peaks
to reject peaks compared to excluding the peaks by rejection.

Filter peaks... Reject peaks...

excludes the peaks from display, permanently excludes peaks from the
integration,

does not exclude the peaks from the excludes the peaks from the calcula-
calculation of the total peak area, tion of the total peak area,

can be reversed. cannot be reversed.

• p 333
 12 Evaluation
12.1 Peak integration
12.1.2 How to perform a peak integration

Peak labels Peaks can be labelled with their retention, sequentially numbered, or be marked
with specific identification names. See table below for an instruction on how to
display peak labels.
The label type can be selected on the Curve Style and Colour tab in the Chromatogram
Layout dialog box. De-select all label options to hide the labels, e.g. for presentations.
The illustration below shows the Chromatogram Layout dialog box with the Curve
Style and Colour tab opened:

How to display The table below describes how to display peak labels:
peak labels
Step Action
1 • Choose Edit:Chromatogram Layout.

or
• Click the Chromatogram Layout icon.

Result: The Chromatogram Layout dialog box opens.

2 Click the Curve Style and Colour tab.

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 Evaluation 12

Step Action
3 Select one or more of the following labelling options in the Peak label
field:
• Number

Result: The peaks will be numbered sequentially.

• Peak Name

Result: Peak names will be displayed. See 12.1.6 How to edit the
peaks on page 351 for information about how to name the peaks.
• Retention

Result: The retention volume or time will be displayed.

• Click OK.

• p 335
 12 Evaluation
12.1 Peak integration
12.1.3 How to optimize the baseline with a morphological algorithm

12.1.3 How to optimize the baseline with a morphological

algorithm

Introduction The first choice when you want to optimize the peak integration is to change the
baseline parameters. This section describes how to optimize the baseline with a
morphological algorithm.

The Morphologic- The Morphological algorithm can be described as a line that follows the
al algorithm chromatogram parallel to the X-axis. Data points for the baseline are created
whenever the line touches the curve, and the points are joined at the end to create
a baseline.
The Morphological algorithm gives the best result in curves with drifting baseline
and peak clusters. The morphological baseline follows the curve faithfully, and a
curve with a baseline at a more even level can be created by subtracting the
morphological baseline.
The Morphological algorithm does not work well if there are negative peaks or if
quantitative data from negative peaks are important in the run.
Note: The Morphological algorithm is the default baseline setting.

How to set a The table below describes how to choose a Morphological algorithm and define
Morphological baseline settings.
baseline

Step Action
1 Select Integrate:Peak Integrate.
Result: The Integrate dialog box opens.

2 Click the Baseline settings button in the Integrate dialog box.

Result: The Settings dialog box opens.

3 • Select the Morphological algorithm.

• Change the Baseline parameters if necessary.

See more information about the parameters below this table.

• Click OK.

Note: The same settings can be edited in the Calculate Baseline dialog box when
a new baseline is created. Choose Integrate:Calculate Baseline to open the dialog
box.

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Morphological al- The parameters for the Morphological algorithm are:

gorithm paramet-
ers • Structure width
• Noise window
• Minimum distance between points

Structure width Structure width determines the length of the straight line that follows the
chromatogram. The default value is set at the widest peak in the chromatogram
multiplied by 1.5.
The illustration below is an example of how a morphological baseline follows the
peaks at the different levels in the curve:

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12.1 Peak integration
12.1.3 How to optimize the baseline with a morphological algorithm

The correct struc- Too low settings

ture width settings
Too low Structure width settings can result in a baseline that reaches too high up
in the peaks of the curve. Sometime a wider peak is not recognized because it
contains a cluster of smaller peaks. The Structure width is then set to a value
according to the largest width of the identified narrower peaks, and must be
increased.
Too high settings
Too high Structure width settings mean that narrower peaks, especially in fluctuating
curves, are not properly followed. This happens when an artifact in a curve is
identified as the widest peak by the morphological algorithm, and then is used to
set the default Structure width value.
The illustration below is an example of baselines using the default morphological
algorithm settings (A) and a morphological algorithm with an increased Structure
width value (B).

Noise window Sometimes you get too many peaks after the peak integration, usually because
noise on the baseline is erroneously detected as peaks.
The solution to this is to increase the Noise window parameter. However, this can
result in peak limits too high up on the peak slopes.
Note: You can also use the Reject peaks function in the Integrate dialog box to
reduce the number of peaks based on the total number of accepted peaks or the
minimum peak height.

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Minimum distance The Minimum distance between points is a measure of the distance between the data
between points points used to generate a baseline. The largest number of data points is produced
at the slopes of the curves. If you increase the Minimum distance between points
value, fewer points will be collected on the slopes.
The illustration below is an example of a baseline (A) that is created with the
Minimum distance between points parameter set at a low value. The number of data
points is reduced when the Minimum distance between points parameter is set to a
higher value (B).

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12.1 Peak integration
12.1.4 How to optimize the baseline with a classic algorithm

12.1.4 How to optimize the baseline with a classic algorithm

Introduction The first choice when you want to optimize the peak integration is to change the
baseline parameters. This section describes how to optimize the baseline with a
classical algorithm.

What is the Clas- The Classic algorithm searches for all parts of the source curve that are longer than
sic algorithm? a defined minimum baseline segment and fall within limiting parameters. Together,
the parameter values define the limits for a rectangular box. A part of the source
curve must fit entirely inside this rectangular box to be identified as a baseline
segment.
The Classic algorithm is particularly useful when you need to integrate curves with
negative peaks and when quantitative data from negative peaks are important.

Classic algorithm The parameters for the Classic algorithm are:

parameters
• Shortest baseline segment
• Noise window
• Max baseline level
• Slope limit

See more information about the parameters below.

How to set a Clas- The table below describes how to set a Classic algorithm and define a baseline.
sic baseline
Step Action
1 Click the Baseline settings button in the Integrate dialog box.
Result: The Settings dialog box opens.

2 • Select the Classic algorithm.

• Change the Baseline parameters.

See more information about the parameters below this table.

• Click OK.

Note: The same settings can be edited in the Calculate Baseline dialog box when
a new baseline is created. Choose Integrate:Calculate Baseline to open the dialog
box.

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Test your paramet- The best way to optimize the baseline is to change the baseline parameters step by
er changes step and then check the resulting baseline after each change. When the desired
effect is accomplished it is best to go back and try a parameter value in between
the two last settings to avoid an unnecessarily low or high value.
How much the values should be changed depends on the cause of the peak
integration problem. The table below is a general guideline.

Baseline parameter Recommended initial change

Shortest baseline segment 20-50%

Noise window 10-30%

Max baseline level Usually not necessary to adjust

Slope limit 25-50%

Note: If necessary, click the Default button to restore the default values.

Shortest baseline If a too high Shortest baseline segment value is set, short curve segments between
segment peaks in the middle of the chromatogram are not identified as baseline segments.
The calculated baseline does not follow the source curve, see below:

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12.1 Peak integration
12.1.4 How to optimize the baseline with a classic algorithm

The Shortest baseline segment value is decreased by 50% in this example:

Slope limit A changed Slope limit will often improve the baseline calculation. The Slope limit
sets the maximum slope of the curve to define when a peak is recognized. A too
high Slope limit will cause the up-slopes of the peaks to be recognized as baseline
segments.
The example above was improved by the shorter baseline segments but the high
slope of the short segments in the region between the second and the fourth peak
still makes the baseline unacceptable. In the example below the Slope limit is
increased by a factor of 2.5, which produces a correct baseline:

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Too high slope A too high Slope limit value can cause peak limits too high up on the peaks. This
limit can be the case when the chromatogram includes a very large flow-through or
solvent peak. The large peak affects the calculation of the default parameters and
leads to too high values for the Slope limit.
Note: A too high value for the Noise window can have the same effect and be caused
by the same situation, often also in combination with a high Slope limit.
Peak limits are defined on peaks in the example below due to the high Slope limit:

The example below has a much lower Slope limit, and a lower Noise window:

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12.1 Peak integration
12.1.4 How to optimize the baseline with a classic algorithm

Noise window Sometimes you get too many peaks after the peak integration, usually because
noise on the baseline is erroneously detected as peaks.
The solution to this is to increase the Noise window parameter. However, this can
result in peak limits too high up on the peak slopes.
The illustration below is an example of noise detected as peaks (A) and the result
of a second peak integration with an increased Noise window (B).

Note: You can also use the Reject peaks function in the Integrate dialog box to
reduce the number of peaks based on the total number of accepted peaks or the
minimum peak height.

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Missing peaks Sometimes obvious peaks are not detected in the peak integration. The probable
cause is that the Noise window is set too high. See the illustration below:

All peaks are detected if the Noise window is decreased, see example below:

Note: Missing peaks can also be caused by improper settings for Reject peaks in
the Integrate dialog box, or Filter peaks in the Chromatogram layout dialog box.

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12.1 Peak integration
12.1.4 How to optimize the baseline with a classic algorithm

When to change In rare cases the top of a broad, flat peak can be incorporated as a baseline segment.
the Max baseline This is one of the very few situations where it is useful to change the Max baseline
level
level. The illustration below is an example:

How to set the The table below describes how to set the Max baseline level.
Max baseline level
Step Action
1 Right-click in the chromatogram and select Marker.
Result: A vertical line is set in the chromatogram. A text box in the
top left corner of the chromatogram displays the X-axis and Y-axis
values of the curve at the point where the vertical Marker line crosses
the curve.

2 • Move the Marker with your mouse.

• Measure the height of the peak you want to exclude from the
baseline.

3 Choose Integrate:Calculate baseline.

4 • Select the Classic checkbox as the Chosen algorithm.

• Type a new value for Max baseline level. Set the level slightly
lower than the value that you measured in step 2.
• Click OK.

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Example of a cor- The illustration below is an example of a correct baseline after the Max baseline
rect baseline level has been changed:

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12.1 Peak integration
12.1.5 How to edit the baseline manually

12.1.5 How to edit the baseline manually

The Edit Baseline You can edit the baseline manually in the Edit Baseline dialog box in the Evaluation
dialog box module:
• Select Integrate:Edit Baseline to display the dialog box.

The Edit Baseline dialog box displays the baseline and the curve it was calculated
from. The baseline points are marked with green squares. Hold the cursor above
the baseline point to display its coordinates. See the illustration below:

How to use the The table below describes how to use the zoom function in the Edit Baseline dialog
zoom function box.

Step Action
1 • Click the Zoom icon.

Result: The cursor is changed into a magnifying glass.

2 • Press and hold the left mouse button.

• Drag the cursor over the area you want to zoom in on.
• Release the mouse button.

Result: The area is enlarged. Right-click and select Reset zoom to

restore the full view.

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How to edit and The table below describes how to edit and insert baseline data points:
insert data points
Step Action
1 Select Integrate:Edit Baseline.
Result: If there are more than one baseline available, the Select
Baseline to Edit dialog box opens. If not, proceed to step 2.
• Select the baseline you want to edit from the list.
• Click OK.

Result: The Edit Baseline dialog box opens

2 • Click the Set Curve Points icon.

Result: The cursor is changed into a cross.

3 Add a data point

• Click the left mouse button to place a new baseline point in the
chromatogram.

Result: A new point is created, marked by a green square. The

baseline curve is redrawn as a spline function based on the old and
the new points. The baseline is guided by the points, but does not
necessarily pass through them.

4 Delete a data point

• Double-click the data point.

or
• Click the data point to select it and click the Delete button.

or
• Right-click the data point and select Delete Point from the shortcut
menu.

Result: The data point is deleted and the curve is redrawn.

5 Move a data point

• Select the data point and drag it to a new position.

Result: The baseline curve is redrawn.

6 Click OK.
Result: The Save Edited Baseline dialog box opens.

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12.1 Peak integration
12.1.5 How to edit the baseline manually

Step Action
7 • Confirm the location and type a new name if necessary.
• Click OK.

Result: The new baseline is saved.

Edited baseline The illustration below is an example of a baseline before and after editing:

How to draw a The table below describes how to force a straight baseline between two points.
straight line
Step Action
1 Select the first of the two points in the point list.

2 Click the Draw straight to next point button.

Result: The baseline is drawn through the points as a straight line.

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12.1.6 How to edit the peaks

Introduction Once a peak table has been generated based on an appropriate baseline, it is possible
to split or join peaks and to manually adjust the peak start and end points. The
peaks will then be renumbered and the peak values will all be recalculated.

How to open the The table below describes how open the peak table for editing. The editing options
peak table for are described below this table:
editing

Step Action
1 • Select Integrate:Edit Peak Table.

Result: If there are more than one peak table available, the Select
Peak Table to Edit dialog box opens. The name of the baseline on
which the peak table was based is displayed at the bottom of the
panel.

2 • Select the peak table from the list and click OK.
• Select one or more Help Curves to be displayed for reference if
necessary.

Result: The Edit Peak Table dialog box opens.

Note: The Edit Peak Table dialog box will be opened immediately if
you select Save and Edit Peak Table as the last step of the peak integ-
ration.

3 Perform the changes (described in the instructions below).

4 Click OK.
Result: The Save Edited Peak Table dialog box opens. The dialog
box displays a suggested name and location for the peak table.

5 Confirm the name and location and click OK.

How to adjust the The baseline can be adjusted graphically (see also 12.1.5 How to edit the baseline
baseline manually on page 348) in the Edit Peak Table dialog box. The table below describes
this:

Step Action
1 • Click the Set Curve Points icon.

Result: The cursor is changed into a cross.

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12.1 Peak integration
12.1.6 How to edit the peaks

Step Action
2 Perform the operations below as desired:
• Click to insert a new data point.
• Double-click on a data point or right-click the point and select
Delete Point from the short-cut menu to delete the point.
• Click a data point and drag the point to a new position to move
the baseline.

Note: Accept negative peaks must be selected before the peak integ-
ration if you want to be able to drag a data point to move the
baseline above the curve.

How to calculate The baseline can be recalculated in the Edit Peak Table dialog box. The table below
a new baseline describes how to do this:

Step Action
1 • Select Baseline:New:Calculate.

or
• Right-click and select New Calculate from the shortcut menu.

Result: The Settings dialog box opens.

2 • Select an algorithm (Morphological is default).

3 • Adjust the Baseline parameters as desired.

or
• Click the Default Values button for the default values.

4 • Click OK.

Result: The baseline is recalculated.

Note: Select Baseline:New:Zero Baseline to replace the calculated baseline with a

zero baseline.

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The Edit Peak The illustration below shows the Edit Peak Table dialog box.
Table dialog box

How to delete a The table below describes how to delete a peak in the Edit Peak Table dialog box:
peak
Step Action
1 • Click the Edit peaks icon.

• Click the peak in the curve or in the peak table to select the peak.

2 • Right-click and select Delete Peaks from the shortcut menu.

or
• Select Edit:Delete Peaks.

Result: The peak is deleted and the remaining peaks are renumbered.

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12.1 Peak integration
12.1.6 How to edit the peaks

How to add color The table below describes how to add a fill color and a pattern to a peak in the
to a peak Edit Peak Table dialog box:

Step Action
1 • Click the Edit peaks icon.

• Move the cursor over the peak you want to edit.

Result: The cursor is changed into a larger arrow.

• Click to select the peak.

2 • Right-click and select Fill Peak from the shortcut menu.

or
• Select Edit:Fill Peak.

Result: The Color and Pattern dialog box opens.

• Select a color and a pattern.

• Click OK.

Result: The peak is filled according to the selections.

Note: The color and pattern selections will override the general Fill settings that
can be selected for all peaks on the Peak Table tab in the Chromatogram Layout
dialog box.

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Peak start and end The beginning of each peak is marked with a drop-line above the curve, and the
points end of each peak is marked with a drop-line below the curve. The illustration below
shows an example of start and end point drop-lines:

Where there are two peaks beside one another, the end of the first peak will be at
the same point as the beginning of the next peak. Thus, there will be a drop-line
below and above the curve at the same point. See the illustration below:

How to split a It is possible to split the peak into two new peaks by inserting a drop-line. The
peak table below describes how to split a peak in the Edit Peak Table dialog box:

Step Action
1 • Click the Edit peaks icon.

• Click the peak in the curve or in the peak table to select the peak.

2 • Right-click and select Split Peak from the shortcut menu.

or
• Select Edit:Split Peaks.

Result: A new drop-line is inserted at the middle point between the

two existing drop-lines and the peak is split.

Note: The area under each new peak will not be the same if the symmetry of the
original peak was not perfect.

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12.1 Peak integration
12.1.6 How to edit the peaks

How to join peaks It is possible to join the areas of adjacent peaks if they are separated by a drop-line.
The table below describes how to join adjacent peaks in the Edit Peak Table dialog
box:

Step Action
1 • Click the Edit peaks icon.

• Click the peak in the curve or in the peak table to select the peak.

2 • Right-click and select Join Left or Join Right from the shortcut
menu.

or
• Select Edit:Join Left or Edit:Join Right.

Result: The original intervening drop-line is removed and all peaks

are renumbered.

How to add peak The table below describes how to add names in the Edit Peak Table dialog box to
names identify the peaks:

Step Action
1 • Click the Edit peaks icon.

• Click the peak in the curve or in the peak table to select the peak.

2 • Right-click and select Peak Name from the shortcut menu.

or
• Choose Edit:Peak name.

or
• Double-click the peak in the peak table or the curve.

Result: The Edit Peak Name dialog box opens. The number and re-
tention of the selected peak is displayed.

3 Type a name in the Peak name textbox and click OK.

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How to adjust The table below describes how to move the drop-lines to adjust the peak area in
peak areas with the Edit Peak Table dialog box.
drop-lines

Step Action
1 • Click the Edit peaks icon.

• Click the peak in the curve or in the peak table to select the peak.

Result: Two vertical bars become superimposed over the drop-lines

that delimit the selected peak. The area between the bars is filled
with a yellow fill pattern.

2 Drag the bars to define the new limits for the selected peak.
Result: The drop-lines are moved and the peak areas are automatic-
ally recalculated.

Note: A drop-line can never be moved beyond another drop-line or beyond a point
where the peak meets the baseline.

How to use the The table below describes how to use the zoom function in the Edit Peak Table
zoom function dialog box.

Step Action
1 • Click the Zoom icon.

Result: The cursor is changed into a magnifying glass.

2 • Press and hold the left mouse button.

• Drag the cursor over the area you want to zoom in on.
• Release the mouse button.

Result: The area is enlarged. Right-click and select Reset zoom to

restore the full view.

The Integrate If needed you can use the selections on the Integrate menu to perform a peak
menu integration in the Edit Peak Table dialog box. This is useful for example if you want
to re-integrate the curve using different settings or integrate only part of a curve
with different settings.
See 12.1.7 How to integrate part of a curve and how to exclude or skim peaks on
page 358 for more information.

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12.1 Peak integration
12.1.7 How to integrate part of a curve and how to exclude or skim peaks

12.1.7 How to integrate part of a curve and how to exclude

or skim peaks

Introduction There are several possibilities to improve the results if the peak integration is
unsatisfactory. This section describes:
• How to select only part of a curve for integration.
• How to exclude peaks.
• How to skim peaks.

These operations can be performed both in the Integrate dialog box in preparation
for the peak integration, or in the Edit Peak Table dialog box to adjust an
unsatisfactory peak integration. This section describes both alternatives.

How to select part The table below describes how to select only a part of a curve for peak integration
of a curve in the Integrate dialog box:

Step Action
1 • Choose Integrate:Peak Integrate.

Result: The Integrate dialog box opens.

• Click the Peak Window button.

Result: The Peak window dialog box opens.

2 • Type new X-axis values for the Left limit and the Right limit.

or
• Drag the vertical cursor lines to define the limits.

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Step Action
3 Click OK.
Result: The baseline will be calculated from the whole curve, but
the calculation of the peak areas is only performed on the selected
section.

How to exclude You can define criteria to exclude peaks from integration. The table below describes
peaks how to define peaks to be excluded in the Integrate dialog box.

Step Action
1 Click the Reject peaks button.
Result: The Reject Peaks dialog box opens.

2 • Select the appropriate checkboxes and type values for height,

width and area.
• Define how many of the largest peaks you want to include.

• Click OK.

How to include Select the Accept negative peaks checkbox of the Integrate dialog box to include
negative peaks negative peaks in the integration.
Result: The negative peaks will be reported as negative areas in the peak table. By
default, negative peaks are not included in the integration.

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12.1 Peak integration
12.1.7 How to integrate part of a curve and how to exclude or skim peaks

Peak skimming vs. The area under a peak can be calculated either using separating drop-lines or peak
drop-lines skimming:
• Drop-lines are vertical marks that split two peaks at the valley. Drop-lines are
used mostly for peaks of relatively similar size. When a peak has a shoulder,
splitting with drop-lines will cause the first peak to lose too much of its area to
the peak that forms its shoulder.
• The Peak skim option can be used to skim off the smaller peak with a straight
line that starts in the valley between the peaks and ends at the other side of the
smaller peak, at the point where the skim line and the curve slope are equal.

The illustration below is an example of how a drop-line (A) and a skimmed peak
(B) affects the area under the main peak and the peak shoulder. The peak shoulder
area is marked in gray:

How to skim The table below describes how to select a ratio to skim peaks in the Integrate dialog
peaks box:

Step Action
1 Select the Peak skim checkbox.

2 Determine the ratio when peak skimming should be applied based

on the relationship in the illustration below:

Note: The default ratio value is 10.

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Step Action
3 Type the ratio value in the text box.

How to integrate Part of a curve can be selected in the Edit Peak Table dialog box and integrated
part of a curve with settings that differ from the rest of the curve. The table below describes how
to do this.

Step Action
1 • Choose Integrate:Edit Peak Table.

Result: The Select Peak Table to Edit dialog box opens.

• Select the peak table to edit and click OK.

Result: The Edit Peak Table dialog box opens.

2 • Click the Peak Window icon.

Result: Two vertical cursor lines are displayed.

• Drag the cursor lines to the beginning and the end of the selected
part of the curve.

Note: All operations described below will only affect the selected
part of the curve.

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12.1 Peak integration
12.1.7 How to integrate part of a curve and how to exclude or skim peaks

Step Action
3 If desired, change the integration parameters:
Reject peaks
• Choose Integrate:Settings.

Result: The Reject Peaks dialog box opens.

• Change the settings as desired and click OK.

Skim peaks
• Choose Integrate:Peak Skim.

Result: The Peak Skim dialog box opens.

• Select the Skim Peaks checkbox and type a ratio.
• Click OK.

4 • Choose Integrate:Peak Integrate.

Result: The selected part of the curve is peak integrated based on

the changed parameters.

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12.1.8 Measurements

Introduction It is possible to determine the coordinates of any point on a curve and to obtain
values for retention and peak height. This is a useful tool for many other functions,
such as for measuring the parameters used in baseline calculations.

Measurement op- Coordinates can be obtained in two ways:

tions
• Through direct measurement.
• From peak table data.

How to make dir- The table below describes how to make direct measurements in a chromatogram:
ect measurements
Step Action
1 Right-click in the chromatogram and select Marker.
Result: A vertical line is set in the chromatogram. A text box in the
top left corner of the chromatogram displays the X-axis and Y-axis
values of the curve at the point where the vertical Marker line crosses
the curve. See the illustration below:

Note: The color of the Marker is the same as the selected curve.

2 Move the Marker with your mouse to display the peak data.

3 Click the curve name legend above the chromatogram to change to

another curve.
Result: The Y-axis is changed to the one corresponding to the new
curve.

4 Right-click and select Marker again to de-select the function.

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12.1 Peak integration
12.1.8 Measurements

How to set a refer- The table describes how to set a reference point:
ence point
Step Action
1 Right-click in the chromatogram and select Set Marker Ref. Point to
define a reference point for the marker position.

2 When the marker is moved from the reference point, the X-axis and
Y-axis values for the new position are displayed together with:
• the new position in relation to the position of the reference point,
• the minimum, maximum and average values for the curve interval
between the reference point and the new position.

How to record a The table below describes how to record a Snapshot of the current curve values:
Snapshot
Step Action
1 • Right-click in the chromatogram and select Snapshot from the
shortcut menu.

Result: The Snapshot dialog box opens.

2 The dialog box displays all the curve data that was current at the
moment the snapshot was taken.
• Click the Save to file button to save the snapshot as an Excel file.
• Click the Print button to print the snapshot.

How to select The retention time and amplitude of any peak can be viewed directly in a peak
peak table data table after an integration. This data and more is selected in the Chromatogram Layout
dialog box. The table below describes how to select peak table data.

Step Action
1 Click the Chromatogram Layout icon.

Result: The Chromatogram Layout dialog box opens.

2 Click the Peak Table tab.

3 • Select the checkboxes on the Select peak table columns list for all
items that you want to display in the table.
• Click OK.

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12.2 Other evaluations

Introduction This section describes how the results can be used for other types of evaluations.

In this section This section contains these sub-sections.

Topic See
Peak purity and peak identification 12.2.1

How to find slope values 12.2.2

How to simulate peak fractionation 12.2.3

How to create curves 12.2.4

How to use the Fraction Histogram 12.2.5

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12.2 Other evaluations
12.2.1 Peak purity and peak identification

12.2.1 Peak purity and peak identification

Introduction Ratios between UV curves measured at different wavelengths give useful information
about peak purity or peak identity.

Peak purity The absorbance ratio can be used to check peak purity. If the peak is pure, the
absorbance spectra are the same over the whole peak and the ratios should therefore
remain constant. The peak is probably not pure if the absorbance ratio is not the
same over the whole peak.
The illustration below shows a simulated chromatogram of two co-eluting
components with differing absorbance spectra and a small difference in retention
time:

Peak identification The absorbance ratio can be used for peak identification. Different compounds
have a specific ratio between absorbancies at different wavelengths.
The illustration below shows a simulated chromatogram of two components with
differences in their absorbance spectra:

How to divide the Both curves must have a baseline close to zero AU before they can be divided. This
curves is achieved with baseline subtraction. The table below describes how to subtract
the baseline from an earlier integration and divide the curves:

Step Action
1 Create a baseline for each UV curve.

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Step Action
2 Select Operations:Subtract.
Result: The Subtract dialog box opens.

3 • Select the UV curve in the first list of curves.

• Select its baseline in the second list of curves.
• Click OK.

Note: You can also subtract corresponding blank runs if there are
blank runs available.

4 Repeat steps 2 and 3 for the second UV curve.

5 Select Operations:Divide.
Result: The Divide dialog box opens.

6 • Select the first result curve from the subtractions in the first list
of curves.
• Select the second result curve from the subtractions in the second
list of curves.

7 Click the checkbox for Threshold and type values for each curve.
This results in the following:
• The quotient is set to 1.0 if either of the sample values is closer
to zero than the threshold value. Very high quotient values are
prevented if division is performed with values close to zero. Very
low quotient values are also prevented.

Note: Default Threshold values are entered by UNICORN. The values

can be changed.

8 Click OK.

How to filter the The resulting curve can be filtered to reduce noise and to remove ghost peaks. The
result curve table below describes how to filter the curve.

Step Action
1 Select Operations:Smooth.
Result: The Smooth dialog box opens.

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12.2 Other evaluations
12.2.1 Peak purity and peak identification

Step Action
2 • Select the Source Chromatogram.
• Select a Filter Type.

Note: The Median filter is recommended to remove noise that appears

as spikes or occurs in a small area of the curve.
• Click OK.

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12.2.2 How to find slope values

Introduction With ÄKTAdesign systems it is possible to only collect peaks during fractionation.
The way to find suitable slope values for a particular run is described in this section.

Where to use The slope values can be used in the Method Editor
slope values
• as StartSlope and EndSlope values in the Peak_FracParameters instruction.
• as parameters for the Watch instruction.

Using slope values for Watch instructions

Conditional Watch instructions can be set up to let the progress of a run be
determined by the events during the run, e.g. start to collect fractions when the
first peak emerges.
The slope of the curve can be set as a condition to satisfy a Watch condition in the
method during the run. It is important to use accurate slope values for the specific
Watch instruction parameter.

A sample run You must first make a separation run with the sample you intend to purify. The
result from this separation run is then used to find the slope values.

Retention scale Time should be used as the X-axis scale for retention.

Step Action
1 Click the Chromatogram Layout icon.

2 • Click the X-axis tab.

• Select Time.
• Click OK.

• p 369
 12 Evaluation
12.2 Other evaluations
12.2.2 How to find slope values

How to differenti- The slope values are measured on a differentiated curve. The table below describes
ate the curve how to create a differentiated curve.

Step Action
1 Select Operations:Differentiate.
Result: The Differentiate dialog box opens.

2 • Select the UV curve you want in the Source chromatogram list.

• Click the First order radio button.
• Click OK.

Result: The differentiated curve opens in the chromatogram.

How to measure Sometimes the differentiated curve must be filtered to reduce noise and ghost peaks
the slope values before the measurements. See section 12.2.1 Peak purity and peak identification
on page 366.
The table below describes how to measure the slope values on the differentiated
curve.

Step Action
1 Click the name of the differentiated curve (above the chromatogram
window) to select the curve.

2 Use the zoom function to magnify the curve over an appropriate

area.

3 Right-click and select Marker from the short-cut menu.

Result: A vertical cursor bar opens in the chromatogram.

4 Place the Marker at the beginning of a peak where you want the
Watch conditions to be fulfilled, i.e. where the slope becomes higher.

5 Read the actual slope value in the active Marker text box in the top
left corner of the chromatogram window.

Note: The unit for the differentiated curve is mAU/min or AU/min. Any Y-axis
value for the differentiated curve is the UV curve slope at the selected retention
point.

Peak fractionation If your system is an ÄKTAdesign system, measure the slope at the beginning and
for ÄKTAdesign the end of the smallest, flattest peak of all the peaks of interest, and use these values.

03-0014-90 • p 370
 Evaluation 12

Illustration: Slope The illustration below shows a measurement of the slope limit after differentiation:
value measure-
ment

• p 371
 12 Evaluation
12.2 Other evaluations
12.2.3 How to simulate a peak fractionation

12.2.3 How to simulate a peak fractionation

Introduction You can create a curve that simulates a peak fractionation to test the outcome
before the actual peak fractionation is run. This section describes how this is done.

How to simulate The table below describes how to simulate a peak fractionation in the Evaluation
a peak fractiona- module.
tion

Step Action
1 Choose Operations:Simulate Peak Fractionation.
Result: The Simulate Peak Fractionation dialog box opens.

2 Select the Source Chromatogram and the curve the simulated peak
fractionation is to be generated for.

3 If necessary, select a Destination Curve and type a new Curve name.

4 Type new values in the Parameters text boxes.

5 Click OK.
Result: The simulated peak fraction curve is displayed on the chro-
matogram.

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 Evaluation 12

12.2.4 How to create curves

Introduction You can draw a curve of your own in the Evaluation module. This section describes
how this is done.
Note: The right to create and rename curves is defined in the user access rights and
may be restricted.

How to create The table below describes how to set up a chromatogram window to create a curve
curves - step 1 in the Evaluation module.

Step Action
1 Open a result file.

2 Select Operations:Create Curve.

Result: The Create Curve dialog box opens.

3 Select one or more Help curves.

4 Select
• minimum and maximum values for the Y-axis.
• appropriate units from the Unit list.

Note: The help curve determines the minimum and maximum values
for the X-axis.

5 Click OK.
Result: The Create Curve chromatogram window opens.

• p 373
 12 Evaluation
12.2 Other evaluations
12.2.4 How to create curves

How to create In the Create Curve dialog box you can also create new units for the curve. The
new units table below describes how this is done.

Step Action
1 Click the New unit button.
Result: The Create New Unit dialog box opens.

2 Type a new unit name and a number of decimal places.

3 Click OK.
Result: The Create New Unit dialog box is closed. The new unit is
now available in the Create Curve dialog box.

How to create The new curve is created in the Create Curve window. The table below describes
curves - step 2 how to work in this window.

Step Action
1 Click the Set Curve Points icon.

2 • Click to insert curve points in the chromatogram.

• Add more points to draw the curve.

Result: A green square marks the new curve point. The curve is
drawn from the previous point. Hold the cursor over the inserted
point to see the coordinates displayed.
Curve mode
• The regular spline mode draws the curve as a smooth line near
but not through every point.
• Click the Spline through checkbox to draw the curve through all
of the curve points.

3 Move a point
• Select the point and drag it to the new position.

Result: The curve is redrawn.

03-0014-90 • p 374
 Evaluation 12

Step Action
4 Delete a curve point
• Double-click the curve point.

or
• Select the point and click the Delete button.

or
• Select the point, right-click and choose Delete Point from the
shortcut menu.

5 • Click the Zoom icon to focus on details in the curve.

Note: Right-click and select Reset zoom to return to the full view.
• Right-click in the chromatogram window and select Marker.
• Position the Marker bar over peaks in the help curve to measure
the coordinates.

Result: The coordinates are displayed in the Marker text box in the
top left corner of the chromatogram.
Note: Click the Marker text box to display the coordinates for the
created curve. Click again to return to the help curve coordinates.

6 Click OK.
Result: The Save Curve dialog box opens.

7 Type a new name if desired and click OK.

• p 375
 12 Evaluation
12.2 Other evaluations
12.2.4 How to create curves

Curve example The illustration below is an example of a curve created by using the Draw Spline
command in the Create Curve chromatogram window.

How to force the In cases where you have created a curve and not selected the Spline through option,
curve through you may want the curve to pass through some of the points that are outside the
points
created curve. The table below describes how to force the curve through these
points:

Step Action
1 • Select the curve point immediately before the curve point you
want to connect to.

2 • Click the Draw straight to next point button.

Result: The curve is adjusted so that it is drawn as a straight line

between the two points.

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 Evaluation 12

12.2.5 How to use the Fraction Histogram

Introduction The Fraction Histogram dialog box in the Evaluation module can be used to create
a curve for the average fraction absorbance.

How to create a The table below describes how to create a Fraction Histogram curve.
Fraction Histo-
gram
Step Action
1 Select Operations:Fraction histogram.
Result: The Fraction histogram dialog box opens.

2 • Select the desired UV curve.

Note: The fractions curve should already be selected on the middle

list. If you have previous pooled fractions and created a pooled
fraction curve, select the desired fraction curve.

3 • Click OK.

Result: The average fraction absorbance values are displayed as a

new curve in the chromatogram.

• p 377
 12 Evaluation
12.3 Automated evaluation procedures

12.3 Automated evaluation procedures

Introduction An evaluation procedure is a recorded sequence of interactive operations in the

Evaluation module, which can be executed for automated data evaluation and report
generation. The concept is similar to the "macro" facilities in other programs. This
section describes how to work with automated evaluation procedures.

In this section This section contains these sub-sections.

Topic See
How to create a new procedure 12.3.1

How to edit a procedure 12.3.2

How to run a procedure 12.3.3

How to rename and remove procedures 12.3.4

03-0014-90 • p 378
 Evaluation 12

12.3.1 How to create a new procedure

Introduction You can use the Procedure Editor to record or create a new procedure. The Procedure
Editor can also be used to view and edit the instructions within a procedure. This
section describes how to use the Procedure Editor to record new procedures.

The Procedure The illustration below shows the Procedure Editor in Record mode.
Editor dialog box

How to record a The table below describes how to record a new procedure.
procedure
Step Action
1 Open the result file in the Evaluation module.

2 Choose Procedures:Record On.

Result: The Procedure Editor dialog box opens in record mode.

3 Minimize the Procedure Editor dialog box.

4 Perform the evaluation steps that the procedure is to contain.

Result: The steps are recorded in the order that they are performed.

• p 379
 12 Evaluation
12.3 Automated evaluation procedures
12.3.1 How to create a new procedure

Step Action
5 Stop the recording
• Choose Procedures:Record Off.

or
• Restore the minimized Procedure Editor dialog box and click the
Stop button.

or
• Restore the minimized Procedure Editor dialog box and select
Control:End Record.

6 Choose File:Save or File:Save As in the dialog box.

Result: The Save As dialog box opens.

7 • Type a name for the new procedure in the Procedure name text
box.
• Select the Global procedure checkbox if desired (see further inform-
ation below).
• Click OK.

Result: The procedure is saved and available for future use.

8 Click the Close button to close the dialog box.

How to create a You can choose to save the new procedure as a Global procedure. This makes the
Global procedure procedure available to all users. The procedure will have (Global) before the name
to designate that it is available to all users.
You must have Edit global list(s) authorization to be able to save Global procedures.

How to build a You can select instructions in the Procedure Editor dialog box to build a complete
procedure with in- procedure step by step. The procedure instructions are described in B.4 Procedure
structions
instructions on page 504. The table below describes how to create a new procedure
with instructions.

Step Action
1 Choose Procedures:Edit:New.
Result: The Procedure Editor opens in Edit mode.

2 • Select an instruction from the Instruction list.

• Type the appropriate parameters in the Parameter field.
• Click Insert.

03-0014-90 • p 380
 Evaluation 12

Step Action
3 Repeat step 2 until the procedure is complete.

4 Choose File:Save.

5 Type a procedure name and click OK.

6 Click the Close button in the Procedure Editor.

• p 381
 12 Evaluation
12.3 Automated evaluation procedures
12.3.2 How to edit a procedure

12.3.2 How to edit a procedure

Introduction Evaluation operations are represented by instructions in the Procedure Editor dialog
box. The instructions can be modified to suit other specific evaluation needs and
be saved for later use. This section describes how to use the Procedure Editor to
edit a procedure.

How to edit a The table below describes how to edit an existing procedure:
procedure
Step Action
1 Select Procedures:Edit:Open.
Result: The Open Procedure dialog box opens.

2 Select the procedure from the list and click OK.

Result: The Procedure Editor opens in Edit Mode.

3 Select an instruction in the procedure window.

Result: The instruction parameters are displayed in the Instruction
and Parameter fields. A short definition of the selected instruction
is displayed at the bottom left corner.

4 Type new values in the Parameter text boxes and click the Replace
button.
Result: The old parameters are replaced by the new parameters.

5 Add a new instruction

• Select the instruction in the procedure immediately before where
you want the new instruction.
• Select a type and an instruction in the Instruction field.
• Type parameter values in the Parameter field.
• Click the Insert button.

Result: The new instruction is inserted after the selected instruction.

6 Remove an instruction
Select an instruction in the procedure and click the Delete button to
remove the instruction from the procedure.

7 Choose File:Save and click the Close button to close the dialog box.

Descriptions of Appendix B.4 Procedure instructions on page 504 contains a list of procedure
the procedure in- instructions with descriptions.
structions

03-0014-90 • p 382
 Evaluation 12

How to add in- If you start recording again you can add more instructions to a procedure that is
structions to a already open in the Procedure Editor:
procedure when
recording • The new instructions will be added to the end of the present procedure.

or
• The new instructions will be inserted after the selected instruction if an
instruction has been selected.

Invalid instruc- The procedure will stop and display an error message if an instruction calls for an
tions invalid operation when the procedure is run. Any subsequent instructions in the
procedure will not be executed.

Address the right Curves are identified only by their storage position. An instruction can become
curves invalid if it addresses the wrong curve:
Example
• The instruction ADD (01,02,03) will try to add curve 01 to curve 02 and store
the result in position 03.
• A curve in position 03 that is not a raw data curve will be overwritten.
• A raw data curve in position 03 cannot be overwritten and the procedure will
be stopped at that point.

Default values for When a classic or morphological algorithm is used to calculate a baseline,
classic baseline in- UNICORN will suggest default values for the four control parameters based on
structions
the appearance of the curve. To instruct UNICORN to use default values
appropriate for the curve every time the procedure is run, choose the default setting
in the appropriate fields for the parameters.
Example
• CALCULATE_BASELINE (01, 06, XXX, XXX, XXX, XXX)

Can be changed to:

• CALCULATE_BASELINE (01, 06, DEFAULT, DEFAULT, DEFAULT,
DEFAULT)

Global procedures It is not advisable to edit existing global procedures. Open the global procedure
instead and save a copy under a new name. Use this copy for editing purposes.

• p 383
 12 Evaluation
12.3 Automated evaluation procedures
12.3.3 How to run a procedure

12.3.3 How to run a procedure

Introduction You can run the saved procedures either for a specific chromatogram or as batch
runs.

How to run a The table below describes how to run a procedure for a specific chromatogram.
single procedure
Step Action
1 Open a result file.

2 Select Procedures:Run.
Result: The Run Procedure dialog box opens.

3 Select the procedure from the list and click OK.

Result: The procedure is executed.

Note: You can also open the procedure in the Procedure Editor dialog box and
choose Control:Run or click the Play button.

Batch runs It is possible to apply an evaluation procedure to a designated batch of result files
if they are not open in the Evaluation module. An open file will not run and an
error message will be displayed.
The batch run is performed in the background of the Evaluation module and the
results of the run are not seen, with the exception of prints and documentation
that are defined as steps in the procedure. For example, batch runs are useful
• to perform integration with the same parameter settings on many results,
• to print a number of results with the same settings.

How to perform a The table below describes how to perform a batch run:
batch run
Step Action
1 Choose Procedures: Batch run.
Result: The Open Procedure dialog box opens.

2 Select the procedure from the list and click OK.

Result: The Batch Run dialog box opens.

03-0014-90 • p 384
 Evaluation 12

Step Action
3 Use the Browse button to find and select the folder to search for
result files and chromatograms.
Note: The search will only be performed in the selected folder. You
can use standard wildcard characters and define restricting search
criteria for the Result and Chromatogram fields. Up to 10 user-defined
search filters can be saved in the drop-menus.

4 Click the Search button.

Result: A list of found chromatograms is displayed.

5 Select the chromatograms you want to perform the run on.

• The Select All button selects all chromatograms.
• The Clear button removes all chromatograms from the list.

6 Click the Run button.

Result: The batch run is performed and any created curve or peak
table will automatically be saved in each result file.

The Batch Run The illustration below is an example of search results in the Batch Run dialog box:
dialog box

• p 385
 12 Evaluation
12.3 Automated evaluation procedures
12.3.3 How to run a procedure

How to batch-run Evaluation procedures combined with batch runs can be a useful tool to produce
reports printed documentation simultaneously for many result files, e.g. for a number of
scouting runs. The table below describes how to create a procedure to batch-run
reports.

Step Action
1 Choose Procedures:Record On to record a procedure.

2 Choose File:Report.
Result: The Generate Report dialog box opens.

3 Choose a report format.

4 Click the Print button as the final instruction.

5 Choose Procedures:Record Off.

6 Save the procedure.

Note: A printing procedure can also be saved with a method to
produce automatic prints at the end of a run.

Note: When for example a batch run is performed, the latest version of the
procedure will be used. However, procedures that are saved with a method are not
affected if the original procedure is edited at a later time.

How to add pro- You can add up to 15 created evaluation procedures to the Procedures menu in
cedures to the the Evaluation module. The table below describes how to add procedures to the
menu
menu:

Step Action
1 Select Procedures:Menu.
Result: The Edit Procedures Menu dialog box opens.

2 • Select the checkboxes of the procedures you want to display on

the menu.
• Click OK.

Result: The selected procedures are included on the Procedures menu.

Remove a procedure
Open the Edit Procedures Menu dialog box and select the checkbox again to de-select
and remove a procedure from the menu.

03-0014-90 • p 386
 Evaluation 12

12.3.4 How to rename and remove procedures

Introduction The procedures that you have created can be renamed or removed from the list of
available procedures. This section describes how this is done.

How to rename a The table below describes how to rename a procedure.

procedure
Step Action
1 Choose Procedures:Edit:Rename.
Result: The Rename Procedure dialog box opens.

2 Select a procedure.
Result: The procedure name is displayed in the New name text box.

3 Type the new name.

4 Click OK.
Result: The procedure name is changed.

How to delete a The table below describes how to delete a procedure.

procedure
Step Action
1 Choose Procedures:Edit:Delete.
Result: The Delete Procedure(s) dialog box opens.

2 Select a procedure.

3 • Click OK.
• Click the Yes button to confirm.

Result: The procedure is deleted.

Global procedures It is not advisable to edit existing global procedures. Open the global procedure
instead and save a copy under a new name. Use this copy for editing purposes.

• p 387
 13 The Analysis module

13 The Analysis module

Introduction This chapter describes how to use the Analysis module. This module is an optional
feature that must be ordered separately and installed after the regular UNICORN
installation.
The Analysis module is accessed in the Evaluation module. The Analysis module
uses functions in the Evaluation module that are presented in the previous chapters.
It is recommended that you are familiar with the contents of those chapters before
you begin with this chapter.

In this chapter This chapter contains these sections.

Topic See
General information about the module 13.1

Quantitation overview 13.2

How to prepare for quantitation 13.3

How to quantitate the sample 13.4

Automated quantitation 13.5

How to measure molecular size 13.6

03-0014-90 • p 388
 The Analysis module 13

13.1 General information about the module

Introduction This section is an overview of the Analysis module including:

• Definitions of terminology that will be used in this chapter.
• A description of how to install the Analysis module.
• A description of the new procedure instructions that become available when
the Analysis module is installed.

Module functions The Analysis module is an optional extra module that adds functionality to the
regular UNICORN Evaluation module. Basically the Analysis module is used:
• to determine the absolute quantity or concentration of a component.
• to determine the molecular size of a component.

Module menus The Analysis module is accessed in the Evaluation module. After the installation,
two new Evaluation module menus are added:
• Quantitate.
• Mol.Size.

Note: The menus are only available when a result file is open in the Evaluation
module.

Quantitate The Quantitate function provides a wide range of techniques for quantitative analysis:
• External standard quantitation
• Internal standard quantitation
• Standard addition
• Recovery calculations

Quantitate uses peak data from standard runs to produce calibration curves which
can then be used to evaluate the amount and concentration of components in a
sample.

Molecular Size The Molecular Size (Mol.Size) function determines the molecular size of components
in a sample. The function uses a molecular size curve prepared from one or more
standards.

• p 389
 13 The Analysis module
13.1 General information about the module

Term definitions The table below lists definitions for some terminology that is used in this chapter.

Term Definition
Amount This specifically refers to the injected
amount. In most cases, the word
"amount" is used as an abbreviation
for "concentration or amount". Both
concentration and injected amount can
be used to produce the calibration
curve. When analyzing the sample,
both amount and concentration are
calculated.

Calibration curve The relationship between amount and

peak size of a component. The relation-
ship can be shown as a curve and as a
mathematical expression.

Level A known amount or concentration of

a standard. The levels are numbered
1-20 in decreasing or increasing order
of concentration.

Molecular size curve The relationship between molecular

size and retention volume for a num-
ber of components. The relationship
can be shown as a curve and as a
mathematical expression.

Molecular size table All necessary data required to determ-

ine the molecular size of one or several
components in a sample. The molecu-
lar size table contains the molecular
size curve.

Peak size Used generally as a common term for

"peak area or peak height".

Peak table The result of a peak integration

presented in tabular form. The peak
table can include, for example, height,
area and retention volume. After the
analysis, the peak table contains the
values for concentration, amount (and
molecular size).

03-0014-90 • p 390
 The Analysis module 13

Term Definition
Quantitation table All necessary data required to quantit-
ate one or several components in a
sample. The quantitation table con-
tains calibration curve(s) and peak
identification settings.

Sample A sample with an unknown concentra-

tion of the component(s) of interest.
The concentration is determined by
Quantitation.
For molecular size calculations, the
sample contains a component or sever-
al components of unknown molecular
size.

Sample run A chromatographic sample run of a

sample to be analyzed.

Spiking The addition of a known quantity of

the component of interest to the
sample prior to the sample preparation
for the run.

Standard A defined concentration of one or

several components. The concentration
does not have to be the same for all
components in the standard. One or
several standards are used to produce
a calibration curve.
For molecular size calculations, the
standard contains components of
known molecular size.

Standard run A chromatographic standard run of a

specific concentration level of a
standard.

How to install the The table below describes how to install the Analysis module.
Analysis module
Step Action
1 Close all other applications.

2 Insert the installation CD in the CD drive.

3 Open My Computer.

• p 391
 13 The Analysis module
13.1 General information about the module

Step Action
4 Double-click the CD drive icon.
Result: The file window opens.

5 Double-click Setup.exe.

6 Follow the instructions on the screen.

7 Remove the CD after the installation is complete.

Note: See the license agreement for information on the legal aspects of the
installation.
The Analysis module in a network
One or several computers in a network may have the Analysis module installed.
The module does not need to be installed on all network computers that run
UNICORN. All installations must be made in accordance with the license
agreement.

03-0014-90 • p 392
 The Analysis module 13

13.2 Quantitation overview

Introduction Quantitation is used to determine the amount or concentration of components in

a sample. This section is an overview over quantitation in general and the four
quantitation techniques that the Analysis module provides. The section also contains
information about the reliability of quantitation.

In this section This section contains these sub-sections.

Topic See
General information about quantitation 13.2.1

External standard quantitation 13.2.2

Internal standard quantitation 13.2.3

Standard addition quantitation 13.2.4

Recovery calculation 13.2.5

General reliability factors for the quantitation techniques 13.2.6

• p 393
 13 The Analysis module
13.2 Quantitation overview
13.2.1 General information about quantitation

13.2.1 General information about quantitation

Introduction This section is a brief presentation of the quantitation techniques that the Analysis
module provides. The section also contains an outline of the steps in a quantitation
and the procedure instructions for quantitation that are added when the Analysis
module is installed.

About quantita- Most quantitation techniques use peak integration data from standards to produce
tion calibration curves. These curves show the relationship between the amount of the
components of interest and the peak sizes at different concentration levels of the
standard. The relationship can be linear, quadratic or point-to-point. Quantitation
is usually based on a number of test runs using a standard at several concentration
levels.
The amount and concentration of the component(s) of interest in the sample are
then determined from the peak size of the component using the calibration curve.
Note: Quantitation should only be performed on chromatograms that have been
integrated and saved. Time is the recommended base unit for quantitation and it
must be used for all integrations.

Quantitation steps The table below describes the general steps in quantitation. The steps are described
in detail in the sections about the different quantitation techniques.

Step Action
1 Run the different concentration levels of the standard.

2 • Integrate the curves to produce peak tables.

• Check the integration.

3 Identify the components for which calibration curves will be pro-

duced.

4 Enter the known concentrations for the different standards to pro-

duce a calibration curve for each selected component.

5 Run the sample and integrate the curve.

6 Let the program calculate the concentration and amount of the

components of interest in the sample.

Note: The steps above do not apply to Standard addition. See "Standard addition
quantitation" below.

03-0014-90 • p 394
 The Analysis module 13

Illustration of the The quantitation work flow is illustrated below.

work flow

The four quantita- The Analysis module provides four different quantitation techniques:
tion techniques
• External standard quantitation
• Internal standard quantitation
• Standard addition quantitation
• Recovery calculation

Each technique is described below.

External standard One or several component(s) of interest are run to produce a calibration curve.
quantitation The amount and concentration of the component in the sample is then determined
from the calibration curve. This technique is fairly simple and usually produces
accurate results.

Internal standard Peak areas of the components of interest are related to the peak area of an internal
quantitation standard added in a fixed amount to each concentration level of the standard and
to the sample. This technique reduces errors that are caused by changes occurring
between the separation runs and is therefore the technique that can produce the
highest precision if a suitable internal standard can be selected.

Standard addition The sample is spiked with a known amount of the component of interest. The areas
quantitation of the spiked and unspiked sample are then compared and the amount in the
unspiked sample is determined. No calibration curves from standards are used.
Only one component can be quantitated. Compared to other techniques, results
can be obtained more quickly when you are performing a small number of sample
runs with standard addition. However, the precision is limited.

• p 395
 13 The Analysis module
13.2 Quantitation overview
13.2.1 General information about quantitation

Recovery calcula- Recovery is used to determine the losses that can occur during the sample
tion preparation process. The sample is spiked with a known amount of the component
of interest. The amount in the spiked sample is then determined from a calibration
curve and is compared with the amount in an unspiked sample. The recovery can
only be determined for one component each time.

Analysis proced- The table below describes the new procedure instructions for quantitation that
ure instructions become available when the Analysis module is installed.

Instruction Description
QUANTITATE The instruction calculates the concen-
tration and amounts in the sample
from a quantitation table.
Amount and concentration columns
will be added to the peak table.

UPDATE The instruction updates a quantitation

table with new data from one standard
concentration level.
The default Limit (+/-) value of 12.5%
will be used. The quantitation table
will not be updated if the peak area or
peak height of the new and the previ-
ous results differ more than the Limit
value.
Note: Either peak area or height is se-
lected for the Limit value.

Default values
The DEFAULT value for the injection value will be taken from the injection volume
reported by the Autosampler A-900 from the method. DEFAULT can only be used
when the injection is performed by the autosampler.
The DEFAULT value for the concentration level for the standard will be taken from
the level entered in the QuantitationData instruction in the method.

03-0014-90 • p 396
 The Analysis module 13

13.2.2 External standard quantitation

General informa- External standard quantitation is based on the use of a standard prepared in a number
tion of concentration levels. A run is performed for each concentration level and
calibration curves are produced to show the relationship between amount and peak
size for each component. The calibration curves are used to quantitate the
components in the sample.
Note: The standard should contain known amounts of all the components that are
to be quantitated in the sample.

How to improve External standard quantitation can be based on the use of a single standard
quantitation concentration level, but the calibration curve is then limited to a linear
through-the-origin relationship. The use of a number of different concentration
levels of the standard broadens the range of the calibration curve. It also allows
the development of non-linear calibration curves and improves precision. Multiple
runs at each level improve precision further.
The description in this section is based on the use of a standard
• that contains two components,
• which is run at three different concentration levels.

How to perform The table below describes briefly how External standard quantitation is performed
External standard (based on the use of a standard which contains two components and which is run
quantitation
at three different concentration levels).

Step Action
1 Perform a run for each standard level.

2 Integrate the curves to produce a peak table for each run.

• p 397
 13 The Analysis module
13.2 Quantitation overview
13.2.2 External standard quantitation

Step Action
3 Use the peak tables from the standard runs to produce a calibration
curve for each component. This curve shows the relationship between
amount and peak size.
Below is a calibration curve for one of the components:

4 Perform a run with the sample and peak integrate the curve.

5 Identify the components of interest by the peak identification settings

from the sample peak table. Use the peak size(s) to calculate the
concentration and amount from the calibration curve(s).

Illustration - how The illustration below describes how the calibration curve is used to determine the
to use the calibra- amount based on the sample peak area.
tion curve

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 The Analysis module 13

Reliability External standard quantitation normally produces accurate results and is fairly
simple. The following reliability factors are specific to the technique.
• Precision is limited by changes that may take place between the runs, for example
column degradation and mobile phase variations.
• There is no compensation for losses of sample during the sample preparation
process prior to analysis.

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 13 The Analysis module
13.2 Quantitation overview
13.2.3 Internal standard quantitation

13.2.3 Internal standard quantitation

General informa- Internal standard quantitation uses peak tables prepared from the standard, similar
tion to the External standard quantitation. However, a fixed quantity of an additional
component is added to every separation run, including the sample. The peak sizes
of the standards and the sample are then related to the peak size of the internal
standards to compensate for any changes that may have occurred between the runs.

General assump- The internal standard technique relies on the assumption that any changes in the
tion injected amount of the component(s) of interest, e.g. due to sample preparation
losses, correspond to equal changes in the injected amount of the internal standard
component.

Advantages Internal standard quantitation reduces errors that are caused by changes in the system
between successive runs with the sample and the standard concentration levels.
For example, there may be unpredictable losses during the sample preparation
procedure or unintentional changes in the amounts that are injected.

What is a suitable A suitable internal standard must meet the following conditions:
internal standard?
• It must be well separated from the components in the sample (not just from the
components of interest).
• It must not be present naturally in the sample(s).
• It must have similar chemical properties to the component(s) of interest.

To be able to compensate for losses during the sample preparation, all the standard
concentration levels must be subjected to the same sample preparation procedure
as the samples.
Note: If there are several components of interest, they must all be chemically similar.

How to perform The table below describes briefly how Internal standard quantitation is performed.
Internal standard
quantitation
Step Action
1 Prepare a series of concentration levels from the standard.

2 Add an additional component, the internal standard, in the same

concentration to all the standards and to the sample.

3 Perform a run for each standard and the sample.

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 The Analysis module 13

Step Action
4 Integrate the curves to produce a peak table for all standard runs
and for the sample.
Result: Each curve contains a peak from the internal standard.
Changes in the size of the internal standard peak indicate changes
in the system.
See illustration below:

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 13 The Analysis module
13.2 Quantitation overview
13.2.3 Internal standard quantitation

Step Action
5 • Plot all peak sizes relative to the size of the internal standard peak
to produce a calibration curve for each component.
• The standard peak area, relative to the internal standard peak
area, is used to produce a point on the calibration curve.

See illustration below:

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 The Analysis module 13

Step Action
6 • Prepare data from the sample in the same way as the data from
the standard runs to produce peak sizes relative to the internal
standard peak size.
• The resulting relative value is applied to the calibration curve to
determine the amount and concentration of the component of
interest.

See illustration below:

Reliability Internal standard quantitation is potentially the most reliable of the quantitation
techniques. However, if the internal standard component is not selected carefully,
the reliability will probably be worse than with the external standard technique.
There are some specific factors that can affect the reliability:
• There is an increased risk of overlap when the extra component (the internal
standard) is added if the sample contains many peaks.
• The addition of the internal standard must be accurate in both the standards
and samples, otherwise, the precision of the quantitation will be reduced
dramatically.

• p 403
 13 The Analysis module
13.2 Quantitation overview
13.2.4 Standard addition quantitation

13.2.4 Standard addition quantitation

General informa- Standard addition quantitation is a simple way to obtain measurements of amount
tion in your sample (concentration is not calculated). It requires only a first sample run
and a second sample run which has been spiked with a known amount of the
component of interest. The technique is straight-forward and relatively fast when
you are running only a few samples. Standard addition can be useful when you
want to use the internal standard technique but do not have a suitable internal
standard.

Disadvantages The disadvantages of Standard addition quantitation are

• its limited precision compared to the external and internal standard techniques
• its lack of ability to measure more than one component.

How to perform The table below describes briefly how Standard addition quantitation is performed.
Standard addition
quantitation
Step Action
1 Perform a sample run.

2 Perform a second run with a sample that has been spiked with a
known quantity of the component of interest prior to the sample
preparation.
See illustration below:

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 The Analysis module 13

Step Action
3 Perform peak integration on both curves in the Evaluation module
to produce a peak table for both the spiked and the unspiked sample.
Result: The difference in peak area between the spiked and the un-
spiked sample represents the peak area from the added amount.

4 With the assumption of a linear proportionality between the peak

area and amount, and with the added amount known, the software
calculates the amount of the component of interest in the sample:

Reliability Standard addition is the least precise of the quantitation techniques since it is
restricted to a single concentration level and the amount in the sample is calculated
by extrapolation. Below are factors that determine if standard addition can be used
with reliable results:
• The component of interest must be completely resolved from all other
components in the chromatogram. Overlapping peaks will produce unreliable
results.
• The peak integration parameters (baseline settings) must be correctly selected.
The default settings will be satisfactory in many cases, but the integration results
have to be checked for all chromatograms.
• The standard addition technique assumes a linear through-the-origin relationship
between the amount of component and peak size. This is a good approximation
for small quantities under normal conditions.
• Standard addition has no way of compensating for changes that are made
between the runs. However, if losses during sample preparation are constant
between the two runs, they may be accounted for by spiking the sample prior
to the sample preparation.
• A spike amount which is of the same order of magnitude as the sample must be
used to maximize precision.
• All the runs must be performed consecutively to reduce systematic errors and
thereby maximize precision.

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 13 The Analysis module
13.2 Quantitation overview
13.2.5 Recovery calculation

13.2.5 Recovery calculation

General informa- Recovery calculation is used to determine losses that can occur during the sample
tion preparation process. Recovery can also be used to determine the recovery factor
of a preparative purification or a chromatographic process. The recovery factor
can only be determined for a single component.
A calibration curve is produced using a concentration series of an external standard.
The calibration range must cover the amount in both the sample and the spiked
sample. Two runs are performed, one with the sample and a second with the sample
that was spiked prior to the sample preparation with a known amount of the
component of interest. Quantitation of the data from the two sample runs allows
the recovery factor of the sample preparation to be calculated.
Note: The recovery is measured as the recovery for the sample preparation, not
for the separation during the chromatographic analysis.

The recovery The recovery factor can be used to manually compensate for losses during sample
factor preparation. The apparent amount in a sample is divided by the recovery factor
to obtain the corrected amount.

How to perform The table below describes briefly how Recovery calculation is performed.
Recovery calcula-
tion
Step Action
1 Perform a run with each level of the standard.

2 Peak integrate the curves to produce a peak table for each level.

3 Use the data from the peak tables to produce a calibration curve.
Note: This is the same process that is used in the External standard
quantitation.

4 Spike a portion of the sample with a known amount of the compon-

ent of interest prior to the sample preparation.

5 Run both the spiked and an unspiked sample.

6 Peak integrate both samples to produce peak tables for the unspiked
sample and the spiked sample.

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 The Analysis module 13

Step Action
7 The amounts for unspiked and spiked sample are calculated from
the calibration curve. The difference between these amounts provides
the apparent amount of the addition.
See illustration below:

8 The ratio of this apparent amount compared to the amount actually

added to the sample determines the recovery of the system.

Example: If 2 mg of the component of interest had been added to

the sample and quantitation indicated an apparent amount added
of 1.6 mg, the recovery factor would then be 0.8.

Reliability Below are some specific factors that determine if the recovery factor result is reliable:
• A spike amount that is of the same order of magnitude as the sample must be
used to maximize the precision.
• It is assumed that the recovery is the same for both the sample and the spiked
sample. However, if the recovery varies according to the amount of the
component of interest, the results are unreliable.

• p 407
 13 The Analysis module
13.2 Quantitation overview
13.2.6 General reliability factors for the quantitation techniques

13.2.6 General reliability factors for the quantitation

techniques

Reliability factors The following factors are valid for all quantitation techniques, except for Standard
addition:
• Quantitation requires that the components of interest are completely resolved
from all other components in the chromatogram. Overlapping peaks will produce
unreliable results.
• The peak integration parameters (baseline settings) must be correctly selected.
The default settings will be satisfactory in many cases, but the integration results
have to be checked for all chromatograms.
• All integrations must be performed using the same X-axis base unit. For highest
reliability, time is the recommended unit.
• The concentration levels of the standard have to be accurately prepared. Errors
in the amount or concentration values will lead to unpredictable results.
• Self-imposed limitations, such as the use of a small number of concentration
levels of the standard, also limits precision.
• Precision is improved by the appropriate choice of the concentration range of
the standard. The range should extend across the presumed amount in the
sample.
• Use of the most appropriate curve model will maximize precision.
• Accuracy is improved if several runs are performed at each level.
• All the runs should be performed consecutively to reduce systematic errors and
thereby maximize precision.

Further informa- Refer to statistical reference books for more detailed information about quantitative
tion analysis. An example is "Statistics for Analytical Chemistry", 3rd Edition 1993,
J.C. Miller and J.N. MIller, Ellis Horwood PTR Prentice Hall.

03-0014-90 • p 408
 The Analysis module 13

13.3 How to prepare for quantitation

Introduction This section describes how to use peak data from standards to prepare quantitation
tables and calibration curves for use with External standard, Internal standard and
Recovery quantitation.

In this section This section contains these sub-sections.

Topic See
Preparations before quantitation 13.3.1

How to create a quantitation table 13.3.2

How to edit and update a quantitation table 13.3.3

• p 409
 13 The Analysis module
13.3 How to prepare for quantitation
13.3.1 Preparations before quantitation

13.3.1 Preparations before quantitation

Description The table below describes the preparations before the quantitation.

Step Action
1 Create a method to be used for all the standard runs. The method
and the injection volume must be the same for all the runs.

2 Perform at least one run for each concentration level of the standard.

3 Peak integrate the curves to produce a peak table for each of the
standard curves.
Note: When integrated, all standards must use the same X-axis base
unit. Time is the recommended unit for the highest reliability.

4 Check that each integration is correct and consistent.

5 Select File:Save to save all the peak tables.

Concentration The standard series should include standard concentrations that extend beyond
levels the lower and upper limits of the sample amount. If an internal standard is used,
the internal standard must be added in the same concentration in all standards.
Methods created from a wizard
If the method is created from a wizard for ÄKTAdesign systems, you may select
the correct standard concentration level in the variable Quantitation_Type. You can
also set the level after the run has been performed. Each level is an alias for a
specific concentration of the standard.
The list below describes how the levels are applied:
• Level 1 should be selected for the standard with the highest or lowest
concentration.
• The levels must be set in consecutive order of changing concentration of the
standard.
• All runs with the same concentration must be given the same level.

Reject irrelevant If many small irrelevant peaks are detected, it may be an advantage to re-integrate
peaks after adjusting the Reject peaks criteria. The number of largest peaks to detect has
a default value of 20 and it may be helpful if this is set to a smaller value.

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 The Analysis module 13

13.3.2 How to create a quantitation table

Introduction The quantitation table contains all the necessary data, such as the calibration
curves, that are needed to quantitate one or several components in a sample. This
section describes how the quantitation table is created.

How quantitation Quantitation tables are created in the same way for both external standard
tables are created quantitation and for recovery calculations. They both use absolute values of
standard peak data.
For quantitation with internal standard, the peak sizes relative to the size of the
internal standard peak are used to create a calibration curve.

Four process steps The creation of the quantitation table can be divided into four steps:
1. Standard data input
2. Component selection and definition
3. Peak identification
4. Calibration curve and quantitation table creation

Step 1 - How to The table below describes how to input the standard data in the Evaluation module.
input the standard
data
Step Action
1 Select Quantitate:Edit Quantitation Table:New on the menu bar.
Result: The New Quantitation Table dialog box opens with the name
of the active chromatogram displayed in the Source chromatogram
field.

• p 411
 13 The Analysis module
13.3 How to prepare for quantitation
13.3.2 How to create a quantitation table

Step Action
2 • Double-click a result file in the Select peak table list if you want
to select a source chromatogram from another result file.

If desired, the standard can be expressed in Concentration instead of

in Amount.
• Click the Concentration checkbox and edit the injection volume
in the Inj. volume field.

Note: The software will always calculate both amount and concen-
tration for the sample.
• Highlight the standard peak table of level 1 on the Peak table(s)
list and click the Select button.

Note: This should be the table for the highest or lowest concentration
of the standard.
Result: The peak table is added to the Level/Peak table(s) list.

3 • The level is automatically copied onto the list if it already was

set in the method. If so, continue with step 4.
• If a level has not been set, the Select Level dialog box opens. Select
1 on the Level menu and click OK.

4 • Click another result file in the Results field and select the new
source chromatogram.

Result: The peak tables associated with this chromatogram are dis-
played on the Peak table(s) list.

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 The Analysis module 13

Step Action
5 • Repeat steps 3 and 4 until all the standard peak tables have been
selected.

Note: Increase the level number for each new standard concentration
in consecutive order of decreasing or increasing concentration.
• Click the Current button at any time to return to the chromato-
gram that was active before you activated Quantitate.
• Highlight unwanted tables on the list and click Remove.
• Click OK to finish the selection.

Result: The Define Component(s) dialog box opens.

Continue to "Step 2, How to select and define components" below
this table.

Standard concen- It is useful to think of each level as an alias for a specific concentration of the
tration levels standard. You can incorporate up to 10 peak tables at each level, prepared from
runs repeated at the same concentration. Quantitate will later allocate each with an
incrementing suffix, e.g. 1:1, 1:2 etc.

The Define Com- The components that will be used to produce the calibration curves are selected in
ponent(s) dialog the Define Component(s) dialog box. Quantitate must be able to identify these
box
components on all levels. This dialog box is used to set the criteria by which peaks
are identified.
The illustration below shows the Define Component(s) dialog box.

• p 413
 13 The Analysis module
13.3 How to prepare for quantitation
13.3.2 How to create a quantitation table

Examine the com- The Define Component(s) dialog box initially displays the components from level
ponents 1:1, that is the peak table from the highest or lowest concentration of the standard.
The Show curve for level list is used to examine the curve for each standard run.
The size of the components are reduced or increased progressively as you select
levels further down on the list, which reflects the decreasing or increasing
concentration of the standard.
If an internal standard has been incorporated, its peak remains about the same size
on each level.
Peaks detected during the peak integration
Each component peak that was detected during the peak integration, i.e. that is
present in the peak table, is identified by a lower triangle (black in level 1:1, green
in other levels). There may be different peaks detected for different levels. Upper
triangles will later identify the peaks that are selected for quantitation.

Step 2 - How to The table below describes how to select and define the components.
select and define
components
Step Action
1 Select level 1:1 in the Show curve for level list and click a peak.
Result: The peak is highlighted in the table.

2 • Double-click the peak.

or
• Click the Include button.

Result: The peak is selected for quantitation, marked with an upper

triangle and "component no." is listed as the Component name. The
selected peak is affected on all levels.
Note: More than one peak can be selected to produce calibration
curves for several components.

3 Highlight the component name and type a new name.

4 Double-click the internal standard peak (if applicable) and type a

new name.

5 Continue to "Step 3, How to identify the peaks" below this table.

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 The Analysis module 13

The Define com- The peak table within the Define Component(s) dialog box has three columns:
ponent(s) peak
table columns • The (absolute) Retention value of the component in level 1:1.
• The width of each component’s window. If you change the width of the window
by adjusting the cursor lines, this is reflected in the Window column.
• The Component name, with the currently selected component highlighted.

Step 3 - How to Description

identify the peaks
When a component is selected, vertical cursor lines show the current identification
window. The software uses this window to search for peaks on other levels and in
the sample runs. A peak found in the window is assumed to be the component of
interest. You can change the limits by dragging a limit cursor line. Both cursor
lines move symmetrically so that the limits center on the component peak.
The window should be set wide enough to include peaks on the other levels despite
minor variations in retention volumes. However, the window should also be narrow
enough to exclude unwanted peaks that will interfere with the quantitation.
Instruction
The table below describes how to adjust the window width for the best results.

Step Action
1 Drag the cursor lines to set the window to a suitable width.

2 • Use the Show curve for level menu to display all levels and check
that the width is suitable (the window width is the same on all
levels).
• Click the lower green or black triangle to display the actual reten-
tion for a peak.

3 Repeat steps 1 and 2 for all selected peaks.

Note: Overlapping windows are not allowed.

4 If necessary, click the Identification settings button to edit the settings.

See "How to adjust the identification settings" below this table.

5 • Click the OK button to accept the default identification settings.

Result: The Quantitation table dialog box opens.

6 Continue to "Step 4, How to create a calibration curve and a

quantitation table" below this table.

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 13 The Analysis module
13.3 How to prepare for quantitation
13.3.2 How to create a quantitation table

Identification set- The criteria by which peaks are identified are set in the Identification Settings dialog
tings box. The criteria are valid for all the selected peaks in the Define Component(s)
dialog box. These settings also affect the information provided in the peak table
in the dialog box.

How to adjust the Description

identification set-
tings By default, peaks are identified by their absolute retention values and the highest
peak maximum within the window. In most cases, it is not necessary to change
these default settings. Peak identification by absolute retention works well when
there has been little or no drift in retention between successive runs of the standard.
Quantitate will find corresponding peaks in these successive runs providing any
drift in retention does not move a peak outside the peak window.
Instruction
If you have drifting retention that makes peak identification difficult you can choose
to identify peaks according to their position relative to a reference peak. The table
below describes how to adjust the identification settings in the Define Component(s)
dialog box.

Step Action
1 Identify a component peak that can be used as the reference.
Note: Choose a peak that is well separated from any other peaks.
This enables the window to be set relatively wide and the system
can accommodate a larger drift in retention value.

2 Click Identification Settings.

Result: The Identification Settings dialog box opens.

See "How to identify peaks within a window" below.

3 Select Relative retention on the Identify peaks on droplist. (See "Abso-

lute and Relative window width" below)

4 Scroll down the Component menu and select the component to be

used as the reference peak.

03-0014-90 • p 416
 The Analysis module 13

Step Action
5 • Type the window width for the reference peak (an absolute value).

Note: Set the width fairly wide to accommodate a larger drift in the
retention value. Make sure that there are no other large peaks
within the window.
• Click OK.

Result: A column for the relative retention is added in the peak table,
Ret/Ref. The column displays the value of each component relative
to the retention value of the reference component. This reference
component is marked Ref. in the Window% column. The Window%
column shows the window width for each peak expressed as a per-
centage of its relative retention value.

How to identify Quantitate must be advised of how the peaks are to be identified if any of the
peaks within a windows includes more than one peak. The second droplist in the Peak identification
window
field of the Identification Settings dialog box offers the following options:
• Highest peak maximum (default).
• Closest to retention, i.e. closest to the center of the window (see the retention
column in the peak table.)
• Maximum peak area.

Examine the nature of the peaks enclosed by the window and select the option that
differs between the wanted and the unwanted peaks. Use Closest to retention if
there are large peaks from components that are not going to be quantitated.
Note: The selection applies to all peaks, even the internal standard and reference
if used.

Absolute and Rel- When the Peak identification is set to Absolute retention, the peak window width
ative window can be displayed as Absolute or Relative. Select the appropriate button in the
width
Identification Settings dialog box.
• Select Absolute to show the window width of each peak in minutes (or the base
volume unit).
• Select Relative to display the width of each component as a percentage of its
retention.

If Peak identification is set to Relative retention, Window is set automatically to

Relative except for the reference peak.

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 13 The Analysis module
13.3 How to prepare for quantitation
13.3.2 How to create a quantitation table

Step 4 - How to When the component selection and identification settings are completed (see Step
create a calibra- 3), the Quantitation table dialog box is opened:
tion curve and a
quantitation table

The table below describes how to enter data for the standards and create a
quantitation table and a calibration curve.

Step Action
1 • Click the IS and Table settings button if you want to use an intern-
al standard or base the calibration curve on peak height (see
"How to select an Internal Standard" below this table).

2 • Verify that the selected components in the Components list are

correct.
- If an internal standard is used, the related component is la-
belled (IS).
- If relative retention has been used, the reference component
is labelled (Ref).
• Click the Define components button to change the components.

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 The Analysis module 13

Step Action
3 • Select the first component at the top of the Components list.

Note: Do not select an internal standard component (if available)

as the amount for this has already been entered and does not change
between the levels.
• Highlight the Amount/Concentration for Level 1.
• Type the amount or concentration of the component in the
standard at this level.

Note: This is the amount corresponding to the injected volume, not

the total amount used when the standard level was prepared.
• Repeat this for the other levels for this component.

4 Click the Curve model radio button for the best curve model:
• Linear (recommended).
• Linear through origin.
• Quadratic.
• Quadratic through origin.
• Point to point.

Result: The curve is displayed in the Calibration curve window. Each

component level is labelled with crosses. If more than one run has
been performed for any level, all points in that level will be shown.
The average of these points is calculated and this value is used to
produce the calibration curve.

5 Repeat steps 3 and 4 for all the remaining components.

Result: The quantitation table is complete with a calibration curve
for each component.

6 • Save the quantitation table and click Close.

or
• Click the Save as button.

Result: The Save quantitation table dialog box opens.

Note: The Save button is used to save updates in an existing quant-
itation table. However, this will overwrite the original table. You
might prefer to use Save as and create a new name for the edited
table to preserve the original.

7 • Specify if the table is to be globally accessible to any user or re-

stricted to your personal user ID. The default is global.
• Type a name in the Quantitation table name field.
• Click the OK button.

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 13 The Analysis module
13.3 How to prepare for quantitation
13.3.2 How to create a quantitation table

How to select an The table below describes how to select an internal standard in the Quantitation
Internal Standard table dialog box.

Step Action
1 Click the IS and Table settings button.
Result: The IS and Table Settings dialog box opens.
The illustration below shows the IS and Table Settings dialog box
with an Internal standard selected.

2 Type the amount and concentration multipliers in the General field.

Note: These values are normally set to 1. See remarks below.

3 Select the internal standard component on the Internal standard peak

droplist.
Note: The default option is Not selected, which is used for external
standard quantitation and measurements of the recovery factor.

4 Type the injected internal standard amount for the standard and
sample runs in the IS amount text box.

5 Select if the quantitation will be based on Area (default) or Height in

the Quantitation peaks field.
Note: Select Height if the peaks are not completely separated from
those of other components.

6 Click OK.

Note: The amount and concentration of the sample are multiplied by the multiplier
values when the calibration curve is applied to a sample. Change the default values
if you want to determine the amount or concentration in the starting volume of
the sample instead of in the injected volume of the sample.

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 The Analysis module 13

Quantitation stat- The Statistics field in the Quantitation table dialog box displays the Correlation and
istics Explained variance values when available.
Click the More button to open the Statistics dialog box for a complete display of
available data.

Statistical reference values

• The correlation (only available for linear models) should be as close as possible
to 1.00.
• The explained variance value should be as close as possible to 100%.

Note that the value is usually rather high even for poor models. A value of 90%
indicates a very poor model.
The explained variance is not shown for curve models that are drawn through the
origin.
Note: If the point-to-point curve model is selected, no statistics are available.

• p 421
 13 The Analysis module
13.3 How to prepare for quantitation
13.3.3 How to edit and update a quantitation table

13.3.3 How to edit and update a quantitation table

How to open an The table below describes how to open an existing quantitation table for editing
existing table in the Evaluation module.

Step Action
1 Select Quantitate:Edit Quantitation Table:Open.
Result: The Open quantitation table dialog box opens.

2 Select a quantitation table from the Quantitation table(s) list.

Note: By default the list will show the quantitation tables that are
globally available. Click the Personal radio button to display the
tables that are restricted to your own user ID.

3 Click OK.
Result: The Quantitation table dialog box opens.

Note: Quantitate includes an update function that can be used to add new peak size
data to an existing quantitation table in a simplified way. This function does not
allow you to redefine components in the Define Component(s) dialog box.

The update func- The update function can be used to add new peak size data to an existing
tion quantitation table. This enables precision to be improved through the use of data
from a number of standard runs. It also simplifies the process of renewing the
calibration curves before each analysis.
Note: The injection volume must always be the same for the new run as it was for
the previous standard runs.

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 The Analysis module 13

The Update The illustration below shows the Update Quantitation Table dialog box.
Quantitation
Table dialog box

How to prepare The table below describes how to open the function and prepare the calibration
the calibration curve for updating.
curve for updating

Step Action
1 Perform a peak integration for the new run and save the result.

2 Select Quantitate:Edit Quantitation Table:Update.

Result: The Update Quantitation Table dialog box opens.

3 • Select the Personal radio button if the table is located in your

personal folder.
• Select the quantitation table that is to be updated in the Quantita-
tion table field.

4 • Double-click the result file in the Select peak table list to access
the new data.
• Click the Current button if you want to use the result file that is
open in the Evaluation module.

5 • Select the chromatogram on the Source chromatogram list.

• Select the peak table that contains the new data in the Peak
table(s) list.

• p 423
 13 The Analysis module
13.3 How to prepare for quantitation
13.3.3 How to edit and update a quantitation table

Step Action
6 • Select the level you wish to update on the Level list.
• If the selected quantitation table is based on concentration, verify
or edit the Inj. Volume field.
• Click OK.

Result: The Update Calibration Curve dialog box opens.

See "How to update a calibration curve" below.

The Update Calib- Data on the selected components for the curve to be updated are shown in the
ration Curve dia- Component name table. When a component is highlighted, its calibration curve is
log box
displayed above in the Calibration curve before update field.
The calibration curve to be updated is shown without taking the new point into
consideration. A new point is shown either in green or red. If it is green, the area
falls within the set Limit (+/-) value and this point will be used for calculation of
the new calibration curve, instead of the old point. If it is red, it falls outside this
range.

03-0014-90 • p 424
 The Analysis module 13

How to update a Peak size deviation

calibration curve
The Deviation column of the Update Calibration Curve dialog box shows how much
the peak size for the proposed new point differs from the existing size. The Limit
(+/-) column displays the set limit for the deviation. The default value is +/- 12.5%
of the existing peak size. You can edit the Limit (+/-) value. Use the Deviation and
limit as radio buttons to specify if both of these columns are expressed in Absolute
or Relative (%) units.
Instruction
The table below describes how to use the Update Calibration Curve dialog box for
calibration curve updates.

Step Action
1 Choose to update by Average or by Replace. The same selection ap-
plies to all components.
See explanations for the options below this table.

2 Select each component table rows in turn and check that the new
point falls within acceptable limits.

3 Click the Statistics after update button.

Result: The Statistics after update dialog box opens.

4 • Use the statistical data to check the curve model.

Note: The old non-updated calibration curve is still shown, but the
statistics apply to the data after the update. If the new point is red,
the statistics shown will be those for the old curve.
• Click OK to close the Statistics after update dialog box.

5 Repeat steps 2-4 for each component.

6 Click OK.
Result: The Update report dialog box opens. This report provides a
summary of the proposed update so that you can assess its viability.
See illustration below.

7 • Click the Print button for a print-out of the Update report.

and/or
• Click Save or Save as to save the updated table.

Update by Aver- The Average option means that the average area value is calculated from the old
age point (representing the average of the old points at this level) together with the
new point. The green point represents the new average value and not the position
of the point from the new peak table.

• p 425
 13 The Analysis module
13.3 How to prepare for quantitation
13.3.3 How to edit and update a quantitation table

Update by Average may be used if you want to increase the precision of the
calibration curve by performing several runs at each level.

Update by Re- The Replace option means that the old point (representing the average of the old
place points at this level) will be replaced with the new point shown in green. The data
for the old point can then not be recovered.
Update by Replace may be used to simplify the process of renewing the calibration
curve before each analysis. Instead of manually producing a new quantitation table,
you may renew an existing table by running all standard levels again and updating
the table with Replace. The old data will then be deleted.

The Update Re- The illustration below shows the Update Report dialog box.
port dialog box

Features
The list below describes some features of the dialog box.
• Components that will not be updated are shown in the column Updated area
(or Updated ratio if an internal standard is used) with the text Out of limit.
• The column Averaged replicates shows the number of points used to calculate
the average area value. After each update by Average, the number is increased
by one. After an update by Replace, the number will be one.
• Nominal reference retention shows the retention for the reference peak in level
1:1.
• Update reference retention shows the retention for the reference peak in the new
peak table.

03-0014-90 • p 426
 The Analysis module 13

How to rename a The table below describes how to rename an existing quantitation table.
quantitation table
Step Action
1 Select Quantitate:Edit Quantitation Table:Rename.
Result: The Rename quantitation table dialog box opens.

2 • Select Personal to display the quantitation tables that are restricted

to your own user ID, if needed.
• Select the quantitation table you wish to rename on the Quantita-
tion table(s) list.
• Click in the Quantitation table name text box and type a new name.
• Click the Rename button.
• Click the Close button.

Note: You must have Edit global list(s) rights to be able to rename a global
quantitation table.

How to delete a The table below describes how to delete an existing quantitation table.
quantitation table
Step Action
1 Select Quantitate:Edit Quantitation Table:Delete.
Result: The Delete quantitation table dialog box opens.

2 • Select Personal to display the quantitation tables that are restricted

to your own user ID, if needed.
• Select the quantitation table you wish to delete on the Quantitation
table(s) list.
• Click the Delete button.
• Click the Yes button to confirm.
• Click the Close button.

Note: You must have Edit global list(s) rights to be able to delete a global
quantitation table.

• p 427
 13 The Analysis module
13.4 How to quantitate the sample

13.4 How to quantitate the sample

Introduction This section describes how to use calibration curves to quantitate samples.
Calibration curves are applicable to external and internal standard quantitation
and to recovery factor measurement. Standard addition measurements are also
described.

In this section This section contains these sub-sections.

Topic See
External and internal standard quantitation 13.4.1

Standard addition quantitation 13.4.2

How to calculate the recovery factor 13.4.3

03-0014-90 • p 428
 The Analysis module 13

13.4.1 External and internal standard quantitation

Introduction This section describes how to perform quantitation in the Evaluation module using
either an external standard or an internal standard.
The processes involved in both external standard and internal standard quantitation
of a sample are very similar. The procedural differences mainly concern the creation
of the quantitation tables. A quantitation table is specific to either external standard
or internal standard quantitation.

Method for the The method that is used for the sample runs must be the same as for the standard
sample runs runs. If the method is created from a wizard or a template for ÄKTAdesign systems,
select Sample in the variable Quantitation_Type on the Variables tab in the Run Setup.

How to prepare The table below describes briefly how to prepare for the quantitation.
for the quantita-
tion
Step Action
1 Prepare a quantitation table for the components of interest.
See 13.3.2 How to create a quantitation table on page 411 for further
information.

2 Perform a sample run.

Note: If internal standard quantitation is used, the internal standard
must have been added to the sample prior to the sample preparation
procedure. The injected amount must be the same as on the standard
levels.

3 Open the sample result file and peak integrate the sample curve to
produce a peak table.
Note: The sample curve must use the same X-axis base unit as the
standards during the integration. Time is the recommended unit for
highest reliability.

4 Select File:Save to save the peak table.

• p 429
 13 The Analysis module
13.4 How to quantitate the sample
13.4.1 External and internal standard quantitation

How to calculate The table below describes how to calculate the amount and concentration in the
the amount and sample.
concentration

Step Action
1 Select Quantitate:Calculate amount and conc.
Result: The Calculate Amount and Concentration dialog box opens.

2 • Select a quantitation table on the Quantitation table droplist.

• Select the chromatogram that contains the sample curve on the
Source chromatogram droplist.
• Select the sample peak table from the Peak table(s) list.
• Check the Injection volume value and type a new value if necessary.

Note: For internal standard quantitation, the injection volume must

be the same as used for the standard runs.
• Click the OK button.

Result: The peak table is updated.

03-0014-90 • p 430
 The Analysis module 13

How to view the The results of the quantitation are shown in the Concentration and Amount peak
quantitation res- table columns of the Evaluation module.The Peak Names are shown in the table
ults
and the type of quantitation is also listed. See illustration below:

The quantitation table used for the quantitation

When the result file is saved, it includes the quantitation table that was used for
the quantitation. You can view the table that was used by selecting Quantitate:Edit
Quantitation Table:View Current.

If the amount can- If the amount cannot be calculated, one of the following signs is shown in the peak
not be calculated table Amount column:

Sign Function
> This means that the value is higher than the highest value in the
calibration curve, i.e. outside the valid range of the calibration curve.

< This means that the value is lower than the lowest value in the calib-
ration curve, i.e. outside the valid range of the calibration curve.

- This means that the value cannot be calculated. For example, this
sign might indicate that the peak could not be identified.

• p 431
 13 The Analysis module
13.4 How to quantitate the sample
13.4.2 Standard addition quantitation

13.4.2 Standard addition quantitation

Stages in standard Standard addition is performed in five stages:

addition
Stage Description
1 Perform two runs.

2 Copy the curves into one result file.

3 Integrate the curves to produce the peak tables.

4 Select the component to be used.

5 Evaluate the amount of a component in the sample.

How to prepare The table below briefly describes how to prepare for the quantitation.
for the Standard
addition quantita-
tion Step Action
1 Perform a sample run with the unspiked sample and a run with the
spiked sample.

2 Open one of the two result files. Use File:Open:Curves to copy the
second curve to the opened result file.

3 Peak integrate the sample curves to produce the peak tables for the
unspiked and the spiked samples.
Note: The sample curves must use the same X-axis base unit. Time
is the recommended unit for highest reliability.

4 • Check that the integrations are correct.

• Optimize the peak integration if necessary.

5 Select File:Save to save the peak table.

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 The Analysis module 13

How to select the The table below describes how to select the component to be used for the standard
component and addition and how to identify the sample peaks.
identify the
sample peaks
Step Action
1 Select Quantitate:Standard addition
Result: The Standard Addition dialog box opens.

2 • Select the chromatogram that contains the peak table for the un-
spiked sample in the Source chromatogram droplist.
• Select the unspiked sample peak table from the Peak table(s) list
to the left.

3 Repeat step 2 in the Addition chromatogram section to the right to

select the addition peak table for the spiked sample.

4 • Edit the default unit mg in the Unit label field if necessary.

• Type the amount of the component that was added as the spike
in the Added amount field.
• Click OK.

Result: The Identify Peak dialog box opens.

5 To locate and select the peak of the unspiked sample, do the follow-
ing:
• Click its triangle marker (black) or select its reference in the
Source table.

6 • Repeat step 5 to select the spiked sample. The triangle color is

blue. Use the Addition table.
• Click the OK button.

• p 433
 13 The Analysis module
13.4 How to quantitate the sample
13.4.2 Standard addition quantitation

The Identify Peak The illustration shows the Identify Peak dialog box, described in the table above.
dialog box

How to view the The amount of the component of interest is displayed in the peak table Amount
quantitation res- columns of the Evaluation module.
ults

03-0014-90 • p 434
 The Analysis module 13

13.4.3 How to calculate the recovery factor

How to prepare The table below briefly describes how to prepare for the quantitation.
for the quantita-
tion
Step Action
1 Prepare a quantitation table for the components of interest.
Note: An external standard quantitation must be used. Internal
standard quantitation tables cannot be used.

2 Perform a sample run with the unspiked sample and a run with the
spiked sample.

3 Peak integrate the sample curves to produce the peak tables for the
unspiked and the spiked samples.
Note: The sample curves must use the same X-axis base unit as the
standards during the integration. Time is the recommended unit for
highest reliability.

4 • Check that the integration is correct.

• Optimize the integration if necessary.

5 • Open one of the sample result files.

• Use File:Open:Peak Tables to copy the other peak table to that
result file.

6 Select File:Save to save the result.

How to calculate The table below describes how to calculate the recovery factor.
the recovery
Step Action
1 Select Quantitate:Calculate Recovery.
Result: The Calculate Recovery Factor dialog box opens.

• p 435
 13 The Analysis module
13.4 How to quantitate the sample
13.4.3 How to calculate the recovery factor

Step Action
2 • Select Global or Personal quantitation tables.
• Select a quantitation table on the Quantitation table droplist.

Note: Only external standard quantitation tables will be shown.

• Select the chromatogram that contains the unspiked sample peak
table on the Source chromatogram droplist.
• Select the unspiked sample peak table from the Peak table(s) list
to the left.

3 • Repeat step 2 to select the peak table for the spiked sample on
the Addition chromatogram fields.
• Select the component that was added prior to the sample prepar-
ation on the Addition component droplist.
• Type the injected amount of this component in the Added amount
field.
• Click the OK button.

How to view the The recovery factor calculated by the software is placed at the bottom of the peak
recovery factor table in the Evaluation module. You need to scroll to the end of the table to see it.
calculation results

Note: The checkbox Do not show global peak table data must be de-selected in the
Peak Table tab of the Chromatogram Layout dialog box.

If the recovery If the recovery cannot be calculated, one of the following signs is shown in the
cannot be calcu- peak table Amount column:
lated

Sign Function
> This means that one of the amounts/concentrations is higher than
the highest value in the calibration curve, i.e. outside the valid range
of the calibration curve.

< This means that one of the amounts/concentrations is lower than

the lowest value in the calibration curve, i.e. outside the valid range
of the calibration curve.

03-0014-90 • p 436
 The Analysis module 13

Sign Function
- This means that the recovery factor cannot be calculated. For ex-
ample, this sign might indicate that the peak could not be identified
in both runs.

• p 437
 13 The Analysis module
13.5 Automated quantitation

13.5 Automated quantitation

Introduction Some method wizards designed for quantitation are available for ÄKTAdesign
systems supplied with Autosampler A-900 or A-905. These can be used to quantitate
a sample automatically or to update a quantitation table.
The procedures described in this chapter are designed for use with the systems
mentioned above.

In this section This section contains these sub-sections.

Topic See
How to set up for automated quantitation 13.5.1

How to perform automated quantitation 13.5.2

How to perform automated update 13.5.3

03-0014-90 • p 438
 The Analysis module 13

13.5.1 How to set up for automated quantitation

Introduction This section describes how to create a quantitation table for automated quantitation.

Basic conditions A quantitation table must be produced from standards before samples can be
for the quantita- quantitated. The list below describes the basic conditions for the quantitation table:
tion table
• The same method must be used for all standard and sample runs.
• Each level is an alias for a specific concentration of the standard.
• All runs with the same concentration must be assigned the same level.
• Level 1 must be selected for the standard with the highest or lowest
concentration.
• The levels must be set in order of decreasing or increasing concentration of the
standard.

How to prepare The table below describes how to prepare the quantitation table for automated
the quantitation quantitation.
table

Step Action
1 • Use the Method Wizard to create a method.
• Select Autosampler from the Injection Technique droplist in the
Sample Injection dialog box.

2 Proceed with the following dialog boxes in the Method Wizard and
click the Finish button on the last dialog box.
Result: The Run Setup opens.

3 Click the Scouting tab. (See illustration below)

• Select the Quantitation_Type variable from the Scouting Variables
dialog box.
• Select other scouting variables of interest, e.g. Sample_ID, Vial_Num-
ber etc.
• Click OK.

4 • Double-click the Quantitation_Type variable table cell.

• Select the correct standard concentration level.

Note: This corresponds to the text instruction QuantitationData. You

can also set this level after the run has been completed. For more
information about scouting see 7.1 How to set up a scouting scheme
on page 176.

• p 439
 13 The Analysis module
13.5 Automated quantitation
13.5.1 How to set up for automated quantitation

Step Action
5 Click the Evaluation Procedures tab.
• Select the Integrate_and_Print procedure.

Result: This procedure will automatically use default baseline settings

and integrate the first UV curve.

6 Save the method.

7 Perform all the standard runs.

8 In the Evaluation module, select Quantitate:Edit Quantitation Table:New.

9 Create a quantitation table manually from the standard runs. See

13.3.2 How to create a quantitation table on page 411.

The Scouting tab The illustration below shows the Scouting tab in the Run Setup, used to enter
standard data, before the standard concentration level is defined.

03-0014-90 • p 440
 The Analysis module 13

13.5.2 How to perform automated quantitation

Instruction The table below describes how to set up sample runs to perform automated
quantitation.

Step Action
1 • Select File:Open in the Method Editor module.
• Select a method that has been used for standard runs in the Open
dialog box. (See 13.5.1 How to set up for automated quantitation
on page 439)
• Click OK.

2 • Click the Scouting tab in the Run Setup.

• Click the Clear All button to clear the scouting scheme.
• Double-click each Quantitation_Type table cell and select Sample
for all sample runs.

3 Click the Evaluation Procedures tab.

• Select only the Quantitate_Sample procedure. (Click the Import
button to import the Quantitate_Sample procedure if it isn’t dis-
played on the list)

Result: This procedure automatically integrates the first UV curve

with default baseline settings and uses the selected quantitation table
to quantitate the sample. The amounts and concentrations are then
printed.

4 Click the Quantitate button.

Result: The Quantitation table dialog box opens.

5 Select the quantitation table from the Global or Personal folder and
click OK.
Result: The quantitation table is copied into the Quantitate_Sample
procedure.
Note: The procedure cannot be executed if a quantitation table has
not been selected. Time must have been selected as the X-axis base
unit.

6 Save the method with a new name.

7 Perform the run(s).

Result: The amount and concentration of the components in the
samples will be printed automatically after each run.

• p 441
 13 The Analysis module
13.5 Automated quantitation
13.5.3 How to perform automated update

13.5.3 How to perform automated update

Introduction This section describes how to update quantitation tables automatically, also in
scouting runs. See also 13.3.3 How to edit and update a quantitation table on page
422.

How to perform The table below describes how to automatically update a quantitation table with
automated update the Replace option (default).
with the Replace
option
Step Action
1 Open a method in the Method Editor.

2 • Click the Scouting tab in the Run Setup.

• Click the Clear All button to clear the scouting scheme.
• Double-click each Quantitation_Type table cell and select the correct
concentration level for the standards.

3 Click the Evaluation Procedures tab.

• Select the Update_Quantitation procedure. (Click the Import button
to import the Update_Quantitation procedure if it isn’t displayed
on the list)

Result: This procedure automatically integrates the first UV curve

with default baseline settings and updates the selected quantitation
table with the new standard. An update report is then printed.

4 Click the Quantitate button.

Result: The Quantitation table dialog box opens.

5 Select the quantitation table from the Global or Personal folder. Time
must be selected as the X-axis base unit.
Result: The quantitation table is copied into the Update_Quantitation
procedure.
Note: You can only perform one run at each level since the old points
in the quantitation table will be replaced after each run.

6 Save the method with a new name.

7 Perform the run(s).

Result: The quantitation table will be updated automatically after
each run.

Note: The quantitation table will not be updated if the peak area or peak height
of the new and the previous results differ more than the Limit value. The Limit value
is defined either for peak area or height.

03-0014-90 • p 442
 The Analysis module 13

How to perform The table below describes how to automatically update a quantitation table with
automated update the Average option.
with the Average
option
Step Action
1 Open a method in the Method Editor.

2 • Click the Scouting tab in the Run Setup dialog box.

• Click the Clear All button to clear the scouting scheme.
• Double-click each Quantitation_Type table cell and select the correct
concentration level for the standards.

3 • Click the Evaluation Procedures tab.

• Select the Update_Quantitation procedure.
• Click the Quantitate button.

Result: The Quantitation table dialog box opens.

4 Select the quantitation table from the Global or Personal folder and
click OK.
Result: The quantitation table is copied into the Update_Quantitation
procedure.

5 Click the Edit button on the Evaluation Procedures tab.

Result: The Procedure Editor dialog box opens. See illustration below.

6 Select the existing UPDATE instruction.

7 Use the scroll bar in the Parameter field to locate the Average or re-
place point droplist.
• Select the AVERAGE option.
• Click the Replace button to the right of the scroll bar.

8 Select File:Close in the Procedure Editor dialog box to return to the

Run Setup.

9 Save the method and perform the runs.

Result: The quantitation table will be updated automatically after
each run. New average values will be calculated from the old points
together with the new points.

Note: The quantitation table will not be updated if the peak area or peak height
of the new and the previous results differ more than the Limit value. The Limit value
is defined either for peak area or height.

• p 443
 13 The Analysis module
13.5 Automated quantitation
13.5.3 How to perform automated update

The Procedure The Procedure Editor dialog box is illustrated below:

Editor dialog box

How to perform It is possible to run both standards and samples in the same scouting run and
automated update continuously update a previously created quantitation table with new values. The
in scouting runs -
step 1 table below describes how to set up the evaluation procedures for the updates.

Step Action
1 Open the same method that was used to create the quantitation table
from the standard runs and open the Run Setup.

2 Click the Evaluation Procedures tab.

3 Select Update_Quantitation and click Quantitate.

Result: The Quantitation table dialog box opens.

4 Select the quantitation table and click OK.

5 Deselect the Update_Quantitation procedure on the Evaluation Proced-

ures tab.

6 Repeat steps 3 to 5 for the Quantitate_Sample procedure.

Note: Make sure that both procedures are deselected after this is
completed. Otherwise they will be run twice.

7 Proceed with the instructions how to edit the instructions (see table
below).

03-0014-90 • p 444
 The Analysis module 13

How to perform The table below describes how to edit the text instructions.
automated update
in scouting runs -
step 2 Step Action
1 Click the Text Instructions icon.

2 Select the last instruction in the method in the Text pane.

3 • Click the Other radio button in the Instructions field of the Instruc-
tion box.
• Select Evaluate on the Instructions list.

4 • Select Update_Quantitation in the Procedure droplist of the Para-

meters field.

• Click the Var... button in the Parameters field.

Result: The Variable Name Definition dialog box opens.

5 Type a variable name, for example Procedure and click OK.

Result: The Evaluate instruction is inserted in the method. By defining
the evaluation procedure as a variable, different procedures can be
selected on the Scouting tab for different scouting runs.

6 Proceed with the instructions on how to set up the scouting runs for
the standards (see table below).

How to perform The table below describes how to set up the scouting runs for the standards.
automated update
in scouting runs -
step 3 Step Action
1 Select View:Run Setup and click the Scouting tab.

2 Click the Define button.

Result: The Scouting Variables dialog box opens.

• p 445
 13 The Analysis module
13.5 Automated quantitation
13.5.3 How to perform automated update

Step Action
3 Edit the scouting variables list to include:
• Procedure
• Vial_Number
• Injection_volume
• Sample_ID
• Quantitation_Type

Note: The Procedure variable will appear at the beginning of the list
of variables, even though the Evaluate instruction is inserted at the
end of the method.

4 Set up all the standard runs in the scouting scheme:

• Select the Update_Quantitation procedure.
• Ensure that Quantitation_Type is set to the correct standard level
for each run.

Result: The quantitation table will now be updated with new values
after each run. Since the runs will be performed with the Replace
(the default selection) option, you can only perform one run at each
level.

5 Proceed with the instructions on how to set up the scouting runs for
the samples in step 4 (see table below).

Note: The quantitation table will not be updated if the peak area or peak height
of the new and the previous results differ more than the Limit value. The Limit value
is defined either for peak area or height.

How to perform The table below describes how to set up the scouting runs for the samples.
automated update
in scouting runs -
step 4 Step Action
1 Select the Quantitate_Sample procedure.

2 Select Sample in the variable Quantitation_Type for all the sample

runs.
Result: All samples will be run automatically and the amount and
concentration of the components of interest will be printed after
each run.
Note: The result files will include an additional chromatogram (la-
belled 12) that contains a small part of the curves collected during
the execution of the evaluation procedure.

03-0014-90 • p 446
 The Analysis module 13

How to change The table below describes how to change the scouting runs so that the quantitation
the scouting runs table is updated by average after each standard run.
to be updated by
average
Step Action
1 • Click the Evaluation Procedures tab in the Run Setup Editor.
• Click the Import button.

Result: The Import dialog box opens.

2 • Select the current method from the Method file menu.

• Highlight Update_Quantitation in the Select field.
• Type a new name, e.g. Update_Average in the Import as text
box.
• Click the Import button.
• Click the Close button.

Result: The new procedure is added to the list of Evaluation Proced-

ures.

3 Select the new procedure and click the Edit button.

Result: The Procedure Editor dialog box opens.

4 Highlight the existing Update instruction.

5 Use the scroll bar in the Parameter field to locate the Average or re-
place point droplist.
• Select the AVERAGE option.
• Click the Replace button.

6 Select File:Close in the Procedure Editor dialog box to return to the

Run Setup.

7 Select the Update_Average procedure and click the Quantitate button.

Result: The Quantitation table dialog box opens.

8 Select the quantitation table and click OK.

9 Deselect all the procedures on the Evaluation Procedures tab, other-

wise they will be run twice.

10 Click the Scouting tab.

• Select the Update_Average procedure for the second and all follow-
ing runs at each standard level concentration.

Note: The Update_Quantitation procedure (Update by Replace) should

still be used for the first run at each level.

• p 447
 13 The Analysis module
13.6 How to measure molecular size

13.6 How to measure molecular size

Introduction The molecular size of components in a sample can be determined by size exclusion
chromatography. A molecular size calibration curve must first be created with
components of known molecular size. The retention is inversely related to the
molecular size.
This section describes how to measure the molecular size.

In this section This section contains these sub-sections.

Topic See
Overview of molecular size determination 13.6.1

How to determine the molecular size 13.6.2

03-0014-90 • p 448
 The Analysis module 13

13.6.1 Overview of molecular size determination

How to create a The table below is a brief description of how to create a molecular size curve.
molecular size
curve
Step Action
1 Perform a run with one or more standards to create a standard curve.
Note: The standards should contain a number of components of
known molecular size and these should extend beyond the size limits
that are expected in the test sample.

2 Peak integrate the standard curve to produce a peak table.

3 Use the peak table from the standard to produce a molecular size
table. Each peak is represented by a retention value.

4 Select the relevant peaks and input data for the corresponding mo-
lecular sizes.
Result: The software plots these values as a molecular size curve.
This curve has an inverse relationship between the logarithm of the
molecular size and retention.

• p 449
 13 The Analysis module
13.6 How to measure molecular size
13.6.1 Overview of molecular size determination

How to calculate The table below is a brief description of how to use the molecular size table to
the molecular size calculate the molecular size of the components in the sample.
in the sample

Step Action
1 Use the sample peak table to obtain retention values for each of the
components of interest.

2 Use the molecular size curve to obtain the molecular sizes of the
components in the sample. The molecular sizes are presented in the
peak table.

03-0014-90 • p 450
 The Analysis module 13

13.6.2 How to determine the molecular size

Introduction This section describes the technique for measuring molecular size in detail.

Before you start Before you create the molecular size curve, you need to do the following:
• Perform chromatographic runs with an appropriate standard with components
of known molecular size. The standard should contain components of sizes that
extend over the range that is expected in the sample. If you are using many
components, it may be better to split them into two or more standard runs.
• Peak integrate the curves to produce peak tables. The standard curves must all
use the same X-axis base unit during the integration. Volume is the recommended
unit for molecular size determination.
• Save the results.

The Molecular This dialog box is used to select the peaks that will be used to produce the molecular
size table dialog size curve. Each curve and its peak table name is color coded. All the available
box
peaks for all the curves are listed together in the Retention/Mol.size table.
Triangles show that a peak has been selected. The name of its source peak table is
shown above the curve window. This is useful when you wish to know which peak
has been selected of two closely spaced peaks from different peak tables.
The illustration below shows the Molecular size table dialog box.

• p 451
 13 The Analysis module
13.6 How to measure molecular size
13.6.2 How to determine the molecular size

How to create and The table below describes how to create and save a molecular size table in the
save a molecular Evaluation module.
size table

Step Action
1 Open a result file and select Mol. Size:Edit Mol. Size Table:New.
Result: The New Molecular Size Table dialog box opens.

2 • Double-click the result file in the Select peak table(s) list.

• Select the source chromatogram on the Source chromatogram
droplist.

3 Highlight a peak table that was prepared from the standard in the
source Peak table(s) list and click the Select button.

4 Repeat step 3 to select more peak tables.

Note: The runs must all have been made under the same conditions.

5 To deselect a table, highlight the table in the Peak table(s) list to the
right and click the Remove button.

6 Repeat steps 2 to 4 to select peak tables from other result files.

7 • Type the appropriate size measurement unit in the Molecular size

unit label field (default kDa).
• Click OK when the Peak table(s) list to the right contains all the
required peak tables.

Result: The Molecular size table dialog box opens.

8 Use one of the following ways to select a peak:

• Click the peak in the curve.
• Click the peak entry in the Retention/Mol. size table.

9 Double-click in the Mol. size column cell and type the known mo-
lecular size from the standard.

03-0014-90 • p 452
 The Analysis module 13

Step Action
10 Repeat step 8 and 9 for all components of known molecular size.

11 To remove unwanted entries, click the peak entry in the table and
click the Exclude button.

12 Select the appropriate curve model in the Curve model field (see "The
molecular size curve" below).

13 Click the Save as button.

Result: The Save molecular size table dialog box opens.

14 • Choose if the table is to be globally accessible to any user or re-

stricted to your personal user ID. The default is global.
• Type a name in the Molecular size table name field.
• Click OK.

The molecular size The molecular size curve shows the relationship between molecular size and the
curve corresponding retention. The curve is plotted from the Retention/Mol. size data that
you have typed in the table as described above. Before this can be done, a curve
model is needed, which describes the relationship between molecular size and
retention. Each of the peaks selected is represented by a point in this curve, which
is drawn according to the best fit that can be achieved using the selected model.
Select one of the available models in the Curve model field:
• Linear
• Linear (logMw) (Theoretically, this is the best choice.)
• Quadratic
• Quadratic (logMw)
• Point to point
• Point to point (logMw)

• p 453
 13 The Analysis module
13.6 How to measure molecular size
13.6.2 How to determine the molecular size

Molecular size With the exception for the two point-to-point models, the molecular size curves
Statistics can be expressed as mathematical expressions. The expressions and related items
can be viewed in the Statistics dialog box.
• Click the More button in the Statistics field of the Molecular size table to open
the dialog box.

The expression is shown at the top of the window, followed by the values for the
constant that it contains.
Statistical reference values
• The correlation value (only for linear models) should be as close to -1.00 as
possible.
• The explained variance value should be as close to 100% as possible.

Note: Explained variance values are usually high. A value of 90% indicates a very
poor model.

How to open an The table below describes how to open an already existing molecular size table for
existing table editing in the Evaluation module.

Step Action
1 Select Mol. Size:Edit Mol. Size Table:Open.
Result: The Open molecular size table dialog box opens.

2 • Select a molecular size table from the Molecular size table(s) list.

Note: By default the list will show the molecular size tables that are
globally available. Click the Personal radio button to display the
tables that are restricted to your own user ID.
• Click OK.

Result: The Molecular size table dialog box opens.

How to rename a The table below describes how to rename an existing molecular size table.
molecular size
table
Step Action
1 Select Mol. Size:Edit Mol. Size Table:Rename.
Result: The Rename molecular size table dialog box opens.

03-0014-90 • p 454
 The Analysis module 13

Step Action
2 • Select Personal to display the tables that are restricted to your
own user ID, if needed.
• Select the molecular size table you wish to rename in the Molecular
size table(s) list.
• Click in the Molecular size table name text box and type a new
name.
• Click the Rename button.

Note: You must have Edit global list(s) rights to be able to rename global tables.

How to delete a The table below describes how to delete an existing molecular size table.
molecular size
table
Step Action
1 Select Mol. Size:Edit Mol. Size Table:Delete.
Result: The Delete molecular size table dialog box opens.

2 • Select Personal to display the tables that are restricted to your

own user ID, if needed.
• Select the molecular size table you wish to delete in the Molecular
size table(s) list.
• Click the Delete button.
• Click the Yes button to confirm.

Note: You must have Edit global list(s) rights to be able to delete global tables.

How to calculate The table below describes how the molecular size curve is used to calculate the
the molecular size molecular sizes of the components in the sample.

Step Action
1 Perform a sample run and peak integrate the curve to produce a
peak table.
Note: The sample curve must use the same X-axis base unit as the
standards. Use volume for molecular size calculations.

• p 455
 13 The Analysis module
13.6 How to measure molecular size
13.6.2 How to determine the molecular size

Step Action
2 Select Mol. Size:Calculate Mol. Size in the Evaluation module.
Result: The Molecular Size dialog box opens.

3 • Select Global or Personal according to the location of the molecular

size table.
• Select the molecular size table on the Molecular size table droplist.
• Select a chromatogram on the Source chromatogram droplist.
• Select a peak table on the Peak table(s) list and click OK.

Result: The results of the molecular size calculation are shown in

the Mol. size peak table column. (See illustration below)

The Mol. size The illustration below shows the Mol. size peak table column.
peak table column

When the result file is saved, it includes the molecular size table that was used for
the molecular size determination. You can view the table that was used by selecting
Mol. Size:Edit Mol. Size Table:View Current.

03-0014-90 • p 456
 The Analysis module 13

If the molecular If the molecular size cannot be calculated, one of the following signs is shown in
size cannot be cal- the peak table Mol. size column:
culated

Sign Function
> This means that the molecular size is larger than the largest size in
the molecular size curve, i.e. outside the valid range of the curve.

< This means that the molecular size is smaller than the smallest size
in the molecular size curve, i.e. outside the valid range of the curve.

- This means that the retention value is negative.

Molecular size The table below describes the new procedure instruction for molecular size
procedure instruc- measurement that becomes available when the Analysis module is installed.
tion

Instruction Description
MOLSIZE The instruction calculates the molecu-
lar sizes from a molecular size curve.
A Mol.size column will be added to
the peak table.

• p 457
 14 System settings

14 System settings

Introduction This chapter describes some of the general system settings.

In this chapter This chapter contains these sections:

Topic See
General information about system settings 14.1

Alarms 14.2

Curves 14.3

03-0014-90 • p 458
 System settings 14

14.1 General information about system settings

System settings The system settings

• define settings for alarms and warnings
• select the data that will be stored in result files

When to change Each system has a set of default settings.

the system settings
• Changes to the default settings should be made when the system is installed.

Certain system settings may need to be adjusted in the following cases:

• If system components are changed: e.g. the alarm and warning limits

• For specific separation runs: e.g. the monitor and curve settings.

Note: Only the settings for the selected components will be shown for strategies
where you select the system components.

How to change The table below describes the two different ways to change the default system
the default settings settings.

Change Effect
To assign a new value to a parameter The specific change is valid only until
within a method. End in the method. After End the
parameter returns to its default setting.
Note: Only some parameters can be
changed in the method.

To assign a new value to the system The new value is valid for all runs and
setting. remains until you change the value
again or return the setting to its de-
fault value. See "How to assign a new
value to a system setting" below.
Like the default values, the new value
can be changed temporarily in a
method.

Note: You must have System settings authorization to assign a new value to an
actual system setting.

• p 459
 14 System settings
14.1 General information about system settings

How to assign a The table below describes how to assign a new value to a system setting in the
new value to a System Control module.
system setting

Step Action
1 Select System:Settings.
Result: The Instructions dialog box for the connected system opens.
The illustration below shows the dialog box opened with the Alarms
group of settings selected.

2 Click the radio button to select one of the following instruction

groups:
• Alarms
• Specials
• Monitors
• Curves

Result: The instructions for the group are displayed. The parameters
are listed below each instruction. The title bar of the dialog box
shows the selected instruction group.

3 • Select a parameter from the list.

• Change the setting value in the Parameters field.

Result: The parameter is updated with the new value in the list.

4 Click the Set Selected Parameter To Strategy Default Value button to

return to the default value (if necessary).
Result: The default setting that was defined in the system strategy
is restored. Only the selected parameters will be restored.

5 Click OK.

Limits for monitor If the system strategy allows, limits for certain monitor signals can be set in the
signals in methods method. These limits will only work locally in the method and override the global
settings as long as the method is in operation. This feature can be used to set the
pH warning threshold to one value during the process operation and another during
the system cleaning.

03-0014-90 • p 460
 System settings 14

14.2 Alarms

Introduction This section is a description of the Alarms system settings.

Alarms and Warn- The Alarms settings define the upper and lower Alarm and Warning limits for process
ings monitor signals.
The table below describes the difference between Alarms and Warnings.

If the signal exceeds... then...

the Alarm limits • an alarm sounds
• an alarm message is displayed
• the process is paused (i.e. the method execution is
suspended and all pumps are stopped)
• the alarm is noted in the logbook.

The situation must be acknowledged and corrected

before the process can be continued.

the Warning limits • a warning message is displayed

• the process continues
• the warning is noted in the logbook.

Note: The message text in an Alarm dialog box and the corresponding text in the
logbook are both color-coded in red. Warning texts are color-coded in orange both
in the dialog box and in the logbook. The text in the logbook is changed into black
when the Alarm or Warning is acknowledged.
Note: The Alarms are not active unless the mode is set to Enabled.

Alarms in a net- Alarms and warning messages are displayed on all stations with a connection to
work the concerned system. This is regardless of the activity that is currently performed
in UNICORN and regardless of the identity and access rights of the current user.
Alarms and warnings can only be acknowledged from the station that is connected
in control mode.

• p 461
 14 System settings
14.2 Alarms

The hysteresis set- The hysteresis setting (not available for ÄKTAdesign systems) for a warning
ting determines to which extent the signal can oscillate up or down from the warning
limit threshold without re-activating a warning.
After the signal has activated a warning, the warning will not be repeated as long
as the signal remains within a window defined by the hysteresis setting above and
below the warning limit. This prevents repeated warnings from noisy or oscillating
signals close to the warning boundary.

Note: Hysteresis is only relevant for warnings, since an alarm puts the system into
Pause mode at the first alarm.

03-0014-90 • p 462
 System settings 14

14.3 Curves

Introduction This section is a short description of the Curves system settings.

The Instructions The illustration below shows the Instructions dialog box with the Curves instructions
dialog box selected.

Curve settings The curve settings determine which monitor signals that will be stored as curves
in the result file. Verify that Store:ON is set in the Instructions dialog box for all
signals that are to be stored.
Warning: If a curve is set to Store:OFF, data from the specific monitor cannot be
displayed in the curves window during a process run. The data will not be recorded
in any way.

Store and Time The table below describes the function of the two curve settings.
between samples
Setting Function
Store (OFF/ON) This setting determines whether the
curve data is stored or not.

Time between samples This setting determines with which

frequency curve data is recorded. It
does not affect the reading frequency
of the actual monitor. Default value
is the shortest possible time between
samples.

• p 463
 15 System maintenance and error reporting

15 System maintenance and error reporting

Introduction This chapter describes the system maintenance and error reporting functions.

In this chapter This chapter contains these sections:

Topic See
System maintenance functions 15.1

How to generate problem reports 15.2

03-0014-90 • p 464
 System maintenance and error reporting 15

15.1 System maintenance functions

Introduction Some strategies support the capacity to view system information for the components
in a chromatography unit. The system information can be used to issue maintenance
warnings for the components. This is featured in the strategies for the lab-scale
ÄKTAdesign family.
This section describes the system maintenance functions.

How to open the The system maintenance functions are controlled in the Maintenance manager dialog
Maintenance box in the System Control module.
manager
• Select System:Maintenance.

Result: The Maintenance manager dialog box opens with the Info tab selected. The
connected chromatography system is scanned for its components. After a while
the components are displayed.
The illustration below shows the Maintenance manager dialog box with the Info
tab selected and general information about the pump displayed:

How to display Click a component in the list to display the component information.
component inform-
ation You can choose two different views:
• General, e.g. serial number, version number etc.
• Specific, e.g. how many hours a pump has been run etc.

• p 465
 15 System maintenance and error reporting
15.1 System maintenance functions

How to set up a The table below describes how to set up a maintenance warning.
maintenance
warning
Step Action
1 Click the Warning tab.

2 Select a component.
• Choose Warning:New.

or
• Right-click the component and select the New option on the
shortcut menu.

3 • Type the appropriate value in the Periodicity field.

• Type a warning text in the Pop up text box.
• Type a name for the warning type in the Name text box.
• Click the Save button.

4 Repeat steps 2 and 3 to set up more warnings.

5 Click the Close button.

How to view the The component that has been set up for a maintenance warning is marked by an
warning paramet- icon and the name of the warning.
ers and counters
• Select the warning to display the parameters.

Counters show the remaining time or number of operations before the next
maintenance warning. See the illustration below:

03-0014-90 • p 466
 System maintenance and error reporting 15

How to reset the The table below describes how to reset the maintenance warning counters.
counters
Step Action
1 • Select System:Maintenance in the System Control module to open
the Maintenance manager dialog box.
• Click the Warning tab.

2 • Select the warning you want to reset on the component list.

• Choose Warning:Edit.

or
• Right-click and select Edit on the shortcut menu.

Result: The Maintenance manager dialog box changes into edit mode
and the text boxes are activated.

3 • Type new text if necessary.

• Click the Reset button.

Result: The Reset parameters dialog box opens.

4 • Click one or both of the Reset buttons to reset the counters.

• Click OK.

• p 467
 15 System maintenance and error reporting
15.1 System maintenance functions

How to acknow- Once a specific Periodicity parameter has been reached, a warning message will be
ledge a warning displayed. The table below describes how to acknowledge the warning.

Step Action
1 The Warning dialog box opens.

2 • Click the Acknowledge button if you have corrected the problem.

• Click the Ignore button if you haven’t corrected the problem.

Note: You will be reminded later about the unsolved problem if you
click the Ignore button.

03-0014-90 • p 468
 System maintenance and error reporting 15

15.2 How to generate problem reports

Introduction UNICORN contains a Generate Report Wizard for registration of errors or problems
that you have detected or that occur during a run. The Generate Report Wizard takes
you through the steps to generate your report.
There are two ways of accessing the Generate Report Wizard:
• From the UNICORN Manager
• From the System Control.

In this section This section contains these sub-sections:

Topic See
How to generate a report from the UNICORN Manager 15.2.1

How to generate a report from the System Control 15.2.2

• p 469
 15 System maintenance and error reporting
15.2 How to generate problem reports
15.2.1 How to generate a report from the UNICORN Manager

15.2.1 How to generate a report from the UNICORN Manager

Introduction The Generate Report Wizard is used to generate problem reports. This section
describes how to generate a problem report from the UNICORN Manager.

Step 1: How to The table below describes how to create a report with the Generate Report Wizard.
create the report
Step Action
1 Select Administration:Create System Report in the UNICORN Manager
module.

2 The first step is a Welcome screen.

• Click the Next button.

Result: The Systems dialog box opens with a list of the available
systems for the logged-on user.
• Select a system for which the report is to be generated and click
the Next button.

Result: The Description dialog box opens.

3 Add the following information in the dialog box:

• a short description of the problem
• the circumstances under which the problem occurs
• the consequences of the problem.

Click the Next button.

Result: The Reproducibility dialog box opens.

4 Specify whether the problem is reproducible or not. Select one of

these alternatives:
• Yes
(Provide a short description in the text box of how the problem
can be reproduced.)
• No
• Unknown.

Click the Next button to proceed to attach example files (see table
below).

03-0014-90 • p 470
 System maintenance and error reporting 15

Step 2: How to at- You can attach result files, method files and/or log files to the problem report.
tach a file
The table below describes how to attach a file:

Step Action
1 The Attachments dialog box is displayed:

2 • Depending on the character of the file to be attached, select the

appropriate tab: Result, Method, System log or Global log.
• Attach the file:
- Click the Add button.
- Select a file in the dialog box and click the Attach or OK button.

Result: The selected file is added to the tab in the Attachments dialog
box.
Note: To remove a file, select the check box and click the Delete
button.

• p 471
 15 System maintenance and error reporting
15.2 How to generate problem reports
15.2.1 How to generate a report from the UNICORN Manager

Step Action
3 To include more information in the report, select the appropriate
check boxes in the System information field. By default, all options
are checked.
Computer & operating system information
A summary of the computer and operating system information, for
example type of processor, processor speed, RAM, hard disk capacity
and printer.
ÄKTA hardware information
A summary of the specific ÄKTAdesign hardware, for example the
instrument and PROM version for every instrument that is connected.
Integrity check
When UNICORN is installed a checksum calculation is performed
on the stationary files (*.dll and *.exe) for the system. An integrity
check means that a new checksum calculation is performed for the
same files in their folders. This new calculated value is compared
with the checksum value obtained during installation. The results
of the comparison are presented in the report and any deviations
are included.
• Click the Next button.

Result: The Generate report dialog box is displayed.

4 Proceed to Step 3: How to generate and save the report below.

Step 3: How to The table below describes how to generate and save the report:
generate and save
the report
Step Action
1 By default, the report is saved in the folder Unicorn\Reports.
If you want to save the report at another location, select a folder in
the tree structure.

2 You also have these options:

• Click the Preview button to open the report in Notepad.
• Click the Print button to print the report without any preview.

3 Click the Finish button to generate and save the report.

03-0014-90 • p 472
 System maintenance and error reporting 15

15.2.2 How to generate a report from the System Control

Introduction The Generate Report Wizard is used to generate problem reports. When an error
message appears in System Control, you can activate the report wizard from the
error message dialog box. The Generate Report Wizard can also be activated anytime
if you choose System:Report.

Step 1: How to When an error message appears in System Control, follow the instructions in this
create the report table to activate the Generate Report Wizard and create a report:

Step Action
1 • Click the Report button in the error message dialog box.

or
• Choose System:Report.

2 The first step is a Welcome screen.

Click the Next button.
Result: The Description dialog box is displayed and shows a list of
the problems/errors that have occurred. All the problems/errors that
have occurred, together with help texts, are automatically recorded
and included in the report.
• If you select a specific error in the Description dialog box, the
appropriate help text is shown in the error message box.

3 Add the following information in the Description dialog box:

• A short description of the problem.
• The circumstances under which the problem occurs.
• The consequences of the problem.

Click the Next button.

Result: The Reproducibility dialog box opens.

4 Specify whether the problem is reproducible or not. Select one of

these alternatives:
• Yes
(Provide a short description in the text box of how the problem
can be reproduced.)
• No
• Unknown.

Click the Next button to proceed to attach example files (see table
below).

• p 473
 15 System maintenance and error reporting
15.2 How to generate problem reports
15.2.2 How to generate a report from the System Control

Step 2: How to at- You can attach method files and/or log files to the problem report.
tach a file
The table below describes how to attach a file:

Step Action
1 The Attachments dialog box is displayed:

2 • Depending on the character of the file to be attached, select the

appropriate tab: Result, Method, System log or Global log.
• Attach a file:
- Click the Add button.
- Select a file in the dialog box and click the Attach or OK button.

Result: The selected file is added to the tab in the Attachments dialog
box.
Note: To remove a file, select the checkbox and click the Delete
button.

03-0014-90 • p 474
 System maintenance and error reporting 15

Step Action
3 To include more information in the report, select the appropriate
check boxes in the System information field. By default, all options
are checked.
Computer & operating system information
A summary of the computer and operating system information, for
example type of processor, processor speed, RAM, hard disk capacity
and printer.
ÄKTA hardware information
A summary of the specific ÄKTAdesign hardware, for example the
instrument and PROM version for every instrument that is connected.
Integrity check
When UNICORN is installed a checksum calculation is performed
on the stationary files (*.dll and *.exe) for the system. An integrity
check means that a new checksum calculation is performed for the
same files in their folders. This new calculated value is compared
with the checksum value obtained during installation. The results
of the comparison are presented in the report and any deviations
are included.
• Click the Next button.

Result: The Generate report dialog box is displayed.

4 Go to step 3 below.

Step 3: How to The table below describes how to generate and save the report.
generate and save
the report
Step Action
1 By default, the report is saved in the folder: Unicorn\Reports.
If you want to save the report in another location, select a folder in
the tree structure.

2 You also have these options:

• Click the Preview button to open the report in Notepad.
• Click the Print button to print the report without any preview.

3 Click the Finish button to generate and save the report.

• p 475
 A Troubleshooting

A Troubleshooting

Introduction This appendix describes different problems which may arise in UNICORN and
how to solve the problems.

In this appendix This appendix contains these sections:

Topic See
Logon A.1

UNICORN access A.2

Methods and method runs A.3

Evaluation A.4

ÄKTAdesign system specific problems A.5

03-0014-90 • p 476
 Troubleshooting A

A.1 Logon

In this section This section describes how to solve the following log on problems:
• Unable to log on to UNICORN
• Error message “Strategy file error”.

Unable to log on The table below describes some log on problems and their solutions:
to UNICORN
Problem description Solution
You have forgotten your password. Ask the system administrator to supply
a new password.

Username and password not accepted • Restore the file USERS30.MPM

You cannot log on although you use from the latest back-up copy
your correct username and password. or
Reason: The file USERS30.MPM in the • reinstall the default user.
folder \UNICORN\SERVER\FIL
could be corrupt.

No user names: Remote station Make sure that the computer is logged
Both these conditions must apply: on to the network before you start
UNICORN.
• The User name drop-down box in
the Logon dialog box is empty. Note: A remote station accesses the
user list directly from the network
• You are trying to log on from a re-
server.
mote station in a network installa-
tion.

No user names: Local station Make sure that the computer is logged
The user list on a local station in a on to the network before starting
network installation is not up to date. UNICORN.
Note: The user list is stored locally on
a local station, and is updated automat-
ically from the network server if the
computer is logged on to the network.

• p 477
 A Troubleshooting
A.1 Logon

Error message The table below describes some problems and their solutions:
“Strategy file er-
ror”
Problem description Solution
Stand-alone installation Reinstall the strategy as described in
If you receive the error message the Administration and technical
“Strategy file error” in a stand-alone manual “Install selected software
installation, the strategy file is prob- components after the initial installa-
ably corrupt. tion”.

Network installation Make sure that the computer is logged

In a network installation, the error on to the network before UNICORN
message “Strategy file error” may ap- is started, so that the strategy file on
pear if you try to create a method for the server disk is accessible.
a system not physically connected to
the computer.

03-0014-90 • p 478
 Troubleshooting A

A.2 UNICORN access

In this section This section describes how to solve the following UNICORN access problems:
• Unable to access certain UNICORN functions
• Connection problems
- Connections are not available
- System is not available
- Error message in a network installation
- You cannot control the system
• Run data Connection in System Control displays a “NO [1]”, “NO [2]” or “NO
[3]”.

Unable to access The table below describes an access problem and its solution:
certain
UNICORN func-
tions Problem description Solution
UNICORN functions to which you do Choose Administration:User Setup in
not have access appear grey in the the UNICORN Manager to change the
menu and cannot be used. user profile.
Note: Contact the system administrat-
or if you are not authorized to change
your user profile.

Connection prob- The table below describes some connection problems and their solutions:
lems
Problem description Solution
The connections are not available. • Check the connection between the
PC and the chromatography sys-
tem.
• Check that the power to the chro-
matography system is turned on.

The connections are not available even • Quit UNICORN.

though • Shut down and switch off the
• the connection between PC and computer.
chromatography system appears to • Switch off the chromatography
be correct system.
• the power is turned on. • Restart the entire system.

• p 479
 A Troubleshooting
A.2 UNICORN access

Problem description Solution

A system is not available when you Check that you have access rights to
attempt to establish a connection. the system. Access rights are not
automatically assigned for a newly
defined system.

You receive the error message “Can- • Check that the local computer to
not connect to system...” in a network which the system is connected is
installation. turned on and logged on to the
network.
• Check that the computer where you
try to establish a connection is
logged on to the network.
• Check that the limit of 8 connec-
tions to the system has not been
exceeded.

You can establish a connection but • Check that no other user has a
cannot control the system, that is the control mode connection.
Manual menu commands in the System • Check that you have sufficient ac-
Control are grey. cess rights to control the system
manually.

Note: The Method Wizard can be used on a local system even if the network
connection is not established.

The Connection The table below describes some connection problems and their solutions:
field in System
Control displays a
“NO [X]” Problem Description Solution
The Connection field in • Check that the UNICORN PC Control board is
the Run data pane in configured according to the settings made during
System Control says the installation of the program. The same Control
“NO [1]” or “NO unit number, Address and IRQ must be set at the
[2]”. Control board, see the Administration and technic-
al manual “Hardware installation”.
• The communication may also fail if there is a
conflict between the UNICORN PC Control board
configurations and other boards in the PC. If so,
select a free Address and a free IRQ during
UNICORN installation and at the Control Board,
see the Administration and technical manual
“Hardware installation”.

03-0014-90 • p 480
 Troubleshooting A

Problem Description Solution

The Connection field in • Choose Administration:System Setup in the UNICORN
the Run data pane in Manager.
System Control says - Select the system with problems in the dialog
“NO [3]”. box and click the Edit button.
- Check that the strategy, computer name and
the control number are correct according to the
installation at the local station which is physic-
ally connected to the system. See the Adminis-
tration and technical manual “System defini-
tions”.
• If you connect remotely to a system
- check that the local station which is physically
connected to the system is turned on
- check that the network is functioning at both
the remote and the local station.
• Check that the limit of eight connections to the
system has not been exceeded.

• p 481
 A Troubleshooting
A.3 Methods and method runs

A.3 Methods and method runs

In this section This section describes how to solve the following method and method run problems:
• Cannot perform Quit or Logoff
• Monitor signals do not appear in the Curves pane in System Control
• Error message “Couldn't create result file... Destination path could not be
found”
• The Method-System Connection dialog box keeps appearing
• The Method Editor window does not fit on the screen
• There are red instructions in a method
• After Windows logout and login you cannot get a system connection
• The Print screen command does not send a copy of the screen to the printer

Cannot perform The table below describes a problems and its solutions:
Quit or Logoff
Problem description Solution
You are unable to perform Quit or Lo- You might be running a Scouting
goff from UNICORN for a connection. method or a MethodQueue. These
functions require a control mode con-
nection in order to start subsequent
cycles correctly.
Action: Stop the Scouting method or
MethodQueue before you quit or log
off.

03-0014-90 • p 482
 Troubleshooting A

Monitor signals The table below describes a problem and its solution:
do not appear in
the Curves panel
in System Control Problem description Solution
Monitor signals do not appear in the • Choose System:Settings in System
Curves pane in System Control. Control

Result: The System Instructions dialog

box opens.
• Choose the Curves group in the In-
structions field.
• Set the Store option to ON.

Signals for which Store is set to ON can

be selected from the View:Proper-
ties:Curves dialog box in System Con-
trol.

Error message The table below describes a problem and its solution:
“Couldn't create
result file... Destin-
ation path could Problem description Solution
not be found”
If you receive the error message This may happen if the specified folder
“Couldn't create result file... Destina- is on the network server and network
tion path could not be found” at the communication has been lost. The
end of a method, the local computer result file is saved in the Failed folder
was unable to access the folder spe- on the local station.
cified in the result file path.

The Method-Sys- The table below describes a problem and its solution:
tem Connection
dialog box keeps
appearing Problem description Solution
If the Method-System Connection dialog Connect the method(s) to the appropri-
box keeps appearing you have some ate system.
method(s) which is not connected to
a system.
Reason: Most likely you have impor-
ted some method(s) with the command
File:Copy from External in the UNICORN
Manager.

• p 483
 A Troubleshooting
A.3 Methods and method runs

The Method Edit- The table below describes a problem and its solution:
or window does
not fit on the
screen Problem description Solution
The Method Editor window does not • The display screen resolution may
fit the screen and has scroll bars. be set to “1024x768x65536” with
Reason: The incorrect font size might “Large fonts”. You need to install
be installed. the “Small fonts”. This requires
that you have the Windows 2000
or Windows XP CD-ROM that was
shipped with your Compaq com-
puter.
• Insert the CD-ROM and follow the
directions on the screen.

Note: Always install the latest service pack after you have installed something from
the Windows 2000/XP CD-ROM.

03-0014-90 • p 484
 Troubleshooting A

There are red in- The table below describes some solutions to syntax error problems:
structions in a
method
Problem description Solution
Red instructions (instructions with a There are several actions that you can
red dot) in a method are syntax errors take:
and may be due to the following: • Check that the method has been
• The method was connected to the connected to the correct system in
wrong system, that is the strategy either of these ways:
of the system is incompatible with - in the System Method Connection
the method. dialog box when you use the
• The method instructions do not Copy from external dialog box
correspond to the components you - in the Save As dialog box in
have chosen for your system. Check Method Editor.
your system components under Ad-
• If the system is custom designed,
ministration:System Setup in the
open the Method Editor, select the
UNICORN Manager.
red instruction and either delete it
• The Copy function was used instead or replace it with a corresponding
of Copy from external when a meth- instruction (if available) from the
od was imported from a diskette. Instruction box. Repeat this for all
• The wrong system may have been red instructions before saving the
selected in the Save As dialog box method.
in the Method Editor.
• You may also have templates not
intended for your system, which
might be the case for custom de-
signed systems.
• The systems strategy has been up-
dated with a new strategy that dif-
fers in the instruction set.

• p 485
 A Troubleshooting
A.3 Methods and method runs

After Windows The table below describes a system connection problem. This applies only to local
logout and login systems, not remote systems:
you cannot get a
system connection
Problem description Solution
You have logged out of Windows Restart the computer in order to ob-
2000 and then logged in again, but tain a system connection in
you cannot get a system connection in UNICORN.
UNICORN.
Reason: If you shut down Windows
2000 with the command Start:Shut-
down:Close all programs and log in as
a different user, you will not be able
to obtain a System Control connection
in UNICORN the next time you or
another user logs on. This is because
the described shutdown procedure
automatically shuts down a number
of processes, including those needed
for system connection. The services
are only started when the computer is
booted up.

Print screen does The table below describes how to solve a printing problem:
not send a copy of
the screen to the
printer Problem description Solution
The Print screen command only makes If you want to print the view on the
a copy of the screen to the clipboard screen, press the <Print Scrn> key and
and not to the default printer. paste the image from the clipboard
into an appropriate program, such as
Microsoft® Paint, and then print out
the image.

03-0014-90 • p 486
 Troubleshooting A

A.4 Evaluation

In this section This section describes how to solve the following evaluation problems:
• Incorrect date and time in the result file
• Evaluation procedure aborts

Incorrect date and The table below describes a problem and its solution:
time in the result
file
Problem description Solution
The result file shows incorrect date Check the system clock setting.
and time. The date and time recorded in the
result file are taken from the PC sys-
tem clock setting.

Evaluation proced- The table below describes a problem and its solution:
ure aborts
Problem description Solution
The evaluation procedure aborts. Instructions in an evaluation proced-
ure refer to curves by identification
number irrespective of the curve
names. Make sure that the curves
processed when the procedure is ex-
ecuted are compatible with those pro-
cessed when it was recorded. An eval-
uation procedure aborts if you try to
store resulting curves at the position
of an original raw data curve.

• p 487
 A Troubleshooting
A.5 ÄKTAdesign system specific problems

A.5 ÄKTAdesign system specific problems

In this section This section describes how to solve the following problems:
• Connected to a system but no system contact
• Flow scheme not displayed properly

Connected to a The table below describes a problem and its solution:

system but no sys-
tem contact
Problem description Solution
You are connected to a system but • Check that the system is turned on.
have no system contact. • Check that all the cable connections
Indications: In the System Control, are intact.
• the option Connection in the Run • If the above actions do not help,
data pane says “Yes”, try to restart both the computer
• the option Instruments says “Scan- and the system.
ning”,
• there is no contact with the system
after a period of waiting.

Flow scheme not The table below describes a problem and its solution:
displayed properly
Problem description Solution
The flow scheme is not displayed Choose Settings:Control Panel: Dis-
properly. play:Settings in the Windows Start
menu to check that you have selected
65536 colors.

03-0014-90 • p 488
 Evaluation functions and instructions B

B Evaluation functions and instructions

Introduction This appendix describes the functions that are implemented in the Evaluation module.

In this appendix This appendix contains these sections:

Topic See
Smoothing algorithms B.1

Baseline calculation theory B.2

Peak table column components B.3

Procedure instructions B.4

• p 489
 B Evaluation functions and instructions
B.1 Smoothing algorithms

B.1 Smoothing algorithms

Introduction This section describes how the smoothing functions are calculated. Choose
Operations:Smooth in the Evaluation module to view and edit the options.

Moving Average The table below describes the process when the Moving Average smoothing algorithm
is used.

Stage Description
1 For each data point in the source curve, the processed curve is calcu-
lated as the average of the data points within a window centered on
the source data point.
• The width of the window is determined by the parameter value,
expressed as number of data points.

2 When the source point is less than half the window size from the
beginning of the end of the curve, the average is calculated symmet-
rically round the source point over as many data points as possible.
• If you increase the window width, the smoothing effect is also
increased.

Note: The filter algorithm only accepts odd integer parameter values between 1
and 151. If an even number has been given, it is incremented by one (1).

Autoregressive The table below describes the process when the Autoregressive smoothing algorithm
is used:

Stage Description
1 The first data point in the source curve is copied to the processed
curve.

2 For each subsequent data point, the previous processed point is

multiplied with the parameter value and added to the current source
data point.

03-0014-90 • p 490
 Evaluation functions and instructions B

Stage Description
3 The result is then divided by the parameter value plus 1 according
to the following formulae:

Where:
tn = current processed point.
tn-1 = previous processed point.
Sn = current source point.
p = smoothing parameter value.
Note: If you increase the parameter value, the smoothing effect is
also increased.

Note: The filter algorithm only accepts integer parameter values between 1 and
25.

Median The table below describes the process when the Median smoothing algorithm is
used.

Stage Description
1 For each data point in the source curve, the processed curve is calcu-
lated as the median of the data points within a window centered on
the source data point.
• The width of the window is determined by the parameter value,
expressed as number of data points.

2 When the source point is less than half the window size from the
beginning of the end of the curve, the median is calculated symmet-
rically round the source point over as many data points as possible.
• If you increase the window width, the smoothing effect is also
increased.
• To completely remove a noise spike, the window width should
in effect be slightly more than twice the width of the spike.

Note: The filter algorithm only accepts odd integer parameter values between 1
and 151. If an even number has been given, it is incremented by one.

• p 491
 B Evaluation functions and instructions
B.1 Smoothing algorithms

Savitzky-Golay The table below describes the process when the Savitzky-Golay smoothing algorithm
is used.

Stage Description
1 The algorithm is based on performing a least squares linear regression
fit of a polynominal of degree k over at least k+1 data points around
each point in the curve to smoothen the data.
The derivate is the derivate of the fitted polynominal at each point.
The calculation uses a convolution formalism to calculate 1st through
9th derivatives.

2 The calculation is performed with the data in low X to high X order.

If the input trace goes from low to high, it is reversed for the calcu-
lation and is re-reversed afterwards.

Note: See Gorry, Peter A, General Least-Squares Smoothing and Differentation by

the Convolution (Savitsky-Golay) Method (Analytical Chemistry 1990, Volume
62, 570-573) for more information on the Savitzky-Golay algorithm.

03-0014-90 • p 492
 Evaluation functions and instructions B

B.2 Baseline calculation theory

Overall process The table below describes the overall process of a baseline calculation.

Stage Description
1 The baseline segments are defined.

2 The baseline points are selected.

3 The baseline is drawn.

Baseline segment Baseline parameters are used to find the baseline segments. The default values for
definition the parameters are determined from the source curve. The baseline segments are
found by different parameters that are based on the type of algorithm that is
selected.
Note: The parameters can be displayed in the Evaluation module if you choose
Integrate:Calculate baseline function. You can also click the Baseline settings button
in the Integrate:Peak integrate dialog box.

Morphological al- The Morphological algorithm searches for all parts of the source curve where:
gorithm
• The curve parts come into contact at both ends of a horizontal line of the length
defined in the Structure width parameter. The default value of this parameter is
based on the widest detected peak in the curve. The horizontal line is moved
along the curve up the peak until it reaches the contact points. The curve parts
below the horizontal line and the line will now form a "curve" with a plateau.
The center point in the plateau formed by the horizontal line will be the data
point for the baseline.
• The data points fulfil the Minimum distance between data points. This parameter
reduces the total number of data points that are created from a curve.

Classic algorithm The Classic algorithm searches for all parts of the source curve where:
• The curve parts are longer than the Shortest baseline segment. This parameter
determines the minimum length for a part of the source curve to be considered
a possible baseline segment.
• The curve has no point outside the Noise window. The noise window is defined
as a rectangular corridor parallel to the slope of the curve and centered on the
first and last points within the currently inspected segment.
• The slope is less than the Slope limit. This limits the maximum slope of the
baseline to differentiate baseline segments from peaks.
• The curve parts are lower than the Max baseline level. This parameter determines
the highest acceptable signal level for the baseline.

• p 493
 B Evaluation functions and instructions
B.2 Baseline calculation theory

Baseline paramet- The baseline parameters can be illustrated as a rectangular box that the source
ers curve has to fit into in order to be identified as a baseline segment, where:
• The length of the box corresponds to the Shortest baseline segment.
• The height of the box corresponds to the maximum level of noise on the baseline
segments. This is referred to as the Noise window.
• The box is allowed to be tilted with a maximum slope corresponding to the
Slope limit.
• The box is not allowed to move up above the Max baseline level.

Baseline paramet- The illustrations below shows the baseline parameters graphically.
ers - illustration

03-0014-90 • p 494
 Evaluation functions and instructions B

Baseline segment The table below describes the baseline segment identification process:
identification
Stage Description
1 The box is virtually moved along the source curve in steps of one
third of the Shortest baseline segment length to look for baseline
segments.

2 A baseline segment is found whenever the currently examined part

of the source curve fits completely within the box.

3 The found baseline segments are joined by connecting adjacent seg-

ments, provided that the slope of the joining lines does not exceed
the Slope limit.

Baseline points When the baseline segments have been defined and joined, they are replaced by
(Classic al- baseline points at the start and end of each segment. The line between these is also
gorithm)
filled with points.
Note: The baseline points are shown as green squares in the Integrate:Edit baseline
function of the Evaluation module.

Baseline drawing The baseline points are used to create the baseline curve using a spline interpolation.
The spline function ensures that the baseline curve is guided by the baseline points.
However, the curve does not necessarily pass through the baseline points. The
baseline will be a smoothly curved function passing close to or through the points.
To reduce the effect of noise at the peak integration, the created baseline is forced
equal to the source curve in every position where the difference between the baseline
and the source curve is small enough. Choose Integrate:Calculate Baseline. If the
Accept negative peaks option is off, the baseline will be forced down to the level
of the source curve whenever the created baseline goes above the source curve.

How to measure You can try to measure the Shortest baseline segment length directly on your
the baseline seg- chromatogram. The table below describes how to do this:
ment (Classic al-
gorithm)
Step Action
1 Locate the shortest segment of the curve that you consider a part of
the baseline.

2 Use the marker box on the chromatogram to measure the length of

the segment.

3 Choose Integrate:Calculate Baseline and insert this value as the

Shortest baseline segment value.

• p 495
 B Evaluation functions and instructions
B.2 Baseline calculation theory

How to measure Curve coordinates can also be used to measure noise levels on the source curve.
noise level (Classic The table below describes how to do this:
algorithm)

Step Action
1 Use the Zoom function to focus on a part of the curve that is repres-
entative for the baseline noise.

2 Select an appropriate Y-axis scale.

3 Measure the Y-axis coordinates.

4 • Calculate the noise range as the difference between the max. and
min. values.
• Add an extra 20%.
• Choose Integrate:Calculate Baseline and insert this value as the
Noise window value.

How to measure The table below describes how to measure the slope at any part of the curve.
the slope limit
(Classic al-
gorithm) Stage Description
1 Select Operations:Differentiate in the Evaluation module.
Result: The Differentiate dialog box opens.

2 • Select the desired source curve.

• Select the First order calculation option.
• Click OK.

Result: The differentiated curve will appear in the active chromato-

gram.

3 Select an appropriate Y-axis scale, right-click and select Marker to

measure the Y-axis values for the differentiated curve with the curve
coordinates function.
Result: The Y-axis value is interpreted as the UV curve slope at the
selected retention point.

4 • Determine the highest slope value of the baseline (non-peak) part

of the curve.
• Add 10%.
• Select Integrate:Calculate Baseline and use this value as the Slope
limit.

Note: If the differentiated curve is very noisy, it can be filtered with a light Moving
average filter in the Operations:Smooth function.

03-0014-90 • p 496
 Evaluation functions and instructions B

B.3 Peak table column components

Introduction This section contains a list of peak parameters with explanations and calculation
formulae when applicable.

Peak parameters - The diagram below illustrates the peak parameters. See the parameter list below
illustration for explanations.

Peak parameter The list below contains descriptions of the peak parameters.
descriptions
Parameter Description
Amount Values calculated by the Analysis
module. (Only available if the Quantit-
ation module is installed.)

Area Calculated as the area between the

curve and baseline, between the peak
start and peak end, time or volume
base. (Gray area in the diagram
above.)

Asymmetry Peak asymmetry (indicator of column

packing). See definition below this
table.

• p 497
 B Evaluation functions and instructions
B.3 Peak table column components

Parameter Description
Baseline height Baseline amplitude at peak start, peak
maximum and peak end. (A, F and G
in the diagram above.)

Capacity factor The capacity factor will only be calcu-

lated when the chromatogram is in
volume base. The total liquid volume,
Vt, must be entered in the Integrate
dialog box for this parameter to be
calculated. See definition below this
table.

Concentration Values calculated by the Analysis

module. (Only available if the Quantit-
ation module is installed.)

Fraction tube id Fraction number at peak start, peak

maximum and peak end.

Height Maximum amplitude above the

baseline. (C-F in the diagram above)

Kav Gel phase distribution constant in gel

filtration. Kav will only be calculated
when a gel filtration column was used
and when the chromatogram is in
volume base. The void volume, V0,
must be entered in the Integrate dialog
box for this parameter to be calcu-
lated. See definition below this table.

Molecular size Values calculated by the Analysis

module. (Only available if the Quantit-
ation module is installed.)

Plate height (HETP) Height equivalent to theoretical plate

and plates/meter. The column height
must be entered in the Integrate dialog
box for this parameter to be calcu-
lated. See definition below this table.

Peak endpoint heights Amplitude above the baseline at left

(A in the diagram above) and right
peak limits (E-G in the diagram
above).

03-0014-90 • p 498
 Evaluation functions and instructions B

Parameter Description
Peak endpoint retention Retention value at peak start and peak
end, time or volume base. (A and G in
the diagram above.)

Peak name Name of the peak.

Percent of total area Peak area as a percent of the total area

under the curve above the baseline.
Time or volume base.
Note: This value can differ in time and
volume base if the flow rate is not
constant throughout the method.

Percent of total peak area Peak area as a percent of the sum of

all integrated peaks.
Note: This value can differ in time and
volume base if the flow rate is not
constant throughout the method.

Resolution Peak resolution. See definition below

this table.

Retention Retention at the peak maximum, time

or volume base. (C in the diagram
above.)

Sigma Standard deviation for a Gaussian-

shaped peak. See definition below this
table.

Type of peak limits Identifies the criteria for peak start and
peak end as either the baseline intersec-
tion or dropline to the baseline or skim
line.

Width Difference in retention between the

peak end and peak start, time or
volume base. (G-A in the diagram
above.)

Width at half height Calculated by taking the maximum

height of the peak above the baseline,
then determining the peak width at
half this value above the baseline.
Time or volume base. (B-D in the dia-
gram above, where BD bisects CF.)

• p 499
 B Evaluation functions and instructions
B.3 Peak table column components

Note: In the Options dialog box in the UNICORN Manager you can select if negative
retentions should be displayed or not. The default selection is that negative retention
is not displayed.

Sigma formula The formula below is used to calculate Sigma.

Where:
• n is the number of data points.
• x is the volume or time value.
• xymax is the volume or time value at the maximum amplitude value.
• Apeak is the area of the peak.

Note: The peak width for a Gaussian peak is (4 x Sigma).

Peak resolution al- The peak resolution is calculated with one of the following three algorithms:
gorithms
1. (VR2 - VR1) / ((Wb2 + Wb1) / 2)
2. (VR2 - VR1) / ((Sigma2 + Sigma1) x 2)
3. ((VR2 - VR1) / (2 x (Wh2 + Wh1))) / 2.354

Where:
• VR1, Wb1, Sigma1 and Wh1 are the retention, width, Sigma and width at half
height of the previous peak.
• VR2, Wb2, Sigma2 and Wh2 are the retention, width, Sigma and width at half
height of the current peak.

Note: The Resolution algorithm variable in the Options dialog box in the UNICORN
Manager determines which of the three algorithms is used. If this variable has the
value 1, 2 or 3, then the algorithm with the corresponding number in the list above
is used. The default value is 3.

How to change The table below describes how to change the peak resolution algorithm in the
the peak resolu- UNICORN Manager.
tion algorithm

Step Action
1 • Choose the Administration:Options... menu item.

Result: The Options dialog box opens.

03-0014-90 • p 500
 Evaluation functions and instructions B

Step Action
2 • Select the desired algorithm number described as described in
Peak resolution algorithms above, in the Resolution algorithm
droplist.

• Click OK.

Result: The dialog box closes and the peak resolution algorithm is
changed.

Note: You must repeat the peak integrations after the change to update the values
based on the new algorithm.

Capacity factor The formula below is used to calculate the Capacity factor.
formula

Where:
• VR = retention volume.
• Vt = total liquid volume.

• p 501
 B Evaluation functions and instructions
B.3 Peak table column components

Kav formula The formula below is used to calculate Kav.

Where:
• VR = retention volume.
• V0 = void volume.
• VC = column volume.

Asymmetry for- The formula below is used to calculate the Asymmetry.

mula
Asymmetry = B / A
Where:
• A is a partial peak width, measured at a percentage of the peak height, for the
leading part of the peak.
• B is a partial peak width, measured at a percentage of the peak height, for the
tailing part of the peak.

How to change the Asymmetry Ratio

The Asymmetry Ratio is selected in the Options dialog box in the UNICORN Manager.
The table below describes how to select a value:

Step Action
1 • Choose the Administration:Options... menu item.

Result: The Options dialog box opens.

2 • Type a ratio value in the Asymmetry Ratio at text box.

• Click OK.

Result: The ratio value is changed and the dialog box closes.

Note: You must repeat the peak integrations after the change to update the values
based on the new asymmetry ratio. The default ratio is 10%.

03-0014-90 • p 502
 Evaluation functions and instructions B

HETP formula The formula below is used to calculate the HETP value.
HETP = L/N

N = 5.54 x (VR/wh)2 assuming a Gaussian peak.

Where:
• N = no. of theoretical plates.
• L = bed height in cm.
• VR = peak retention (elution) volume or time.
• wh = peak width at half height expressed in the same units as VR.

• p 503
 B Evaluation functions and instructions
B.4 Procedure instructions

B.4 Procedure instructions

Introduction This section contains lists of procedure instructions with descriptions. These
instructions are used in the Procedure Editor. Choose Procedures:Edit:New in the
Evaluation module to view the Instruction list.

Curve operation The table below contains a list of instructions for curve operations.

Instruction Description
ADD Adds two curves to produce a third
curve, which is the sum of the two
curves. The two source curves must
have the same Y-axis unit and not be
fraction or injection curves, or else a
run time error will occur.

AMP_MUL Multiplies the amplitude of the source

curve by the multiplication factor and
stores the result in the target curve
position.

AMP_SHIFT Shifts the amplitude of the source

curve by the shift factor and stores the
result in the target curve position.

CLEAR Clears the specified curve from the

working memory of the computer.

COPY Copies the source curve to the target

curve position.

CUT Cuts out the part of the source curve

between the Left and Right limits and
stores the result in the target curve
position.

DERIVATE Differentiates the source curve (first

or second order) and stores the result
in the target curve position. The Y-
axis of the target curve position will
be a normalized scale without unit.

03-0014-90 • p 504
 Evaluation functions and instructions B

Instruction Description
DIV Divides two curves to produce a third
curve, which is the quotient of the two
curves. The two source curves can
have any Y-axis unit. The Y-axis of
the target curve position will be a
normalized scale without unit.

HISTOGRAM Creates a histogram from any non-

fraction curve (source curve 1) and a
fraction curve (source curve 2_frac),
and stores the result in the target curve
position. If source curve 2 is not a
fraction curve a run time error will
occur. The Y-axis of the target curve
position will be the same as that of the
first source curve.

INTEGRATE Performs a mathematical integration

of the source curve and stores the res-
ult in a Result curve. This instruction
is not the same as Peak integrate,
which performs a real peak integra-
tion.

POOL_FRACTIONS Pools fractions from the source curve

and stores the result in the target curve
position. The fractions are pooled
from the first selected fraction to the
last selected fraction. If the source
curve is not a fraction curve, or First
or Last is not an existing identification,
a run time error will occur.

RET_MUL Multiplies the retention of the source

curve by the Multiplication factor and
stores the result in the target curve
position.

RET_SHIFT Shifts the retention of the source curve

by the Shift factor and stores the result
in the target curve position.

SIMULATE_PEAK_FRAC Simulates Peak Fractionation.

• p 505
 B Evaluation functions and instructions
B.4 Procedure instructions

Instruction Description
SMOOTH_AR Smooths the source curve with an
autoregressive filter and stores the
result in the target curve position. The
Filter parameter decides the strength
of the filter.

SMOOTH_MA Smooths the source curve with a

moving average filter and stores the
result in the Resulting Curve. The Filter
width parameter decides how many
samples wide the filter is.

SMOOTH_MEDIAN Smooths the source curve with a medi-

an filter and stores the result in target
curve position. The Filter width para-
meter decides how many samples wide
the filter is.

SMOOTH_SG Smooths the curve with the Savitzky-

Golay algorithm.

SUB Subtracts two curves to produce a

third curve, which is the difference of
the two curves. The two source curves
must have the same Y-axis unit and
not be fraction or injection curves.

TDIV Divides two curves to produce a third

curve, which is the quotient of the two
curves. The two source curves can
have any Y-axis unit. The threshold
values are used to avoid division of
numbers close to zero. At those points
where source curve 1 has an amplitude
less than Threshold1, or the source
curve 2 has an amplitude less than
Threshold2, the result of the division
is defined to be 1.0.

03-0014-90 • p 506
 Evaluation functions and instructions B

Integration The table below contains a list of instructions for integration.

Instruction Description
CALCULATE_BASELINE Calculates a baseline from the source
curve. The baseline is stored in the
target curve position. DEFAULT can be
selected in the Baseline parameters,
which will then calculate default
baseline parameters for each new
curve.

CALCULATE_BASELINE_MORPH Calculates a baseline from the curve

crvSrc using a morphological method.
DEFAULT can be selected in the Baseline
parameters, which will then calculate
default baseline parameters for each
new curve. The baseline is stored in
curve crvDst.

CLEAR_PEAKTABLE Clears the peak table in Peak table

source from the computer memory.

COPY_PEAKTABLE Copies a peak table from Peak table

source to Resulting peak table.

NEGATIVE_PEAKS Controls the baseline behavior in sub-

sequent baseline calculations. If ONOFF
is ON then the baseline can be drawn
above the curve and negative peaks
can be detected by PEAK_INTEGRATE.
If ONOFF is OFF then the baseline is
never drawn above the curve.

PEAK_INTEGRATE Performs a peak integration on the

source curve and stores the resulting
peak table in Resulting peak table. It
is assumed that the baseline is subtrac-
ted.

PEAK_WINDOW Specifies which part of the source

curve that will be integrated. Peaks
between retention Left limit and Right
limit will be detected if the ONOFF
parameter is set to ON. If ONOFF is set
to OFF, the whole curve will be used
for integration.

• p 507
 B Evaluation functions and instructions
B.4 Procedure instructions

Instruction Description
REJECT_PEAKS Any combination of conditions is al-
lowed. If all parameters are OFF then
every detected peak is included in the
peak table.

SET_COLUMN_HEIGHT Sets the column height for the peak

integration calculation of the HETP
value. The Column height parameter is
the height of the column in centi-
metres. If Column height is OFF then the
HETP value is not calculated for the
following integrations.

SET_COLUMN_V0 Sets void volume for Kav peak integra-

tion calculation.

SET_COLUMN_VT Sets the total liquid volume for peak

integration calculation of the capacity
factor.

SET_SKIM_SIZE_RATIO Sets the Skim size ratio to be used in

the following peak integration(s).

WINDOW_PEAK_INTEGRATE Integrates the curve within the peak

window. All curve parts outside the
peak window remain unchanged.

File operation The table below contains a list of instructions for file operations.

Instruction Description
CURVE_OPEN Opens the curve specified in the Result
file defined in File name and stores it
in target curve position. If "*" is
entered as File name the current result
file will be used. The File name para-
meter may include a path from the
users root folder.

IMPORT_CURVE Imports a curve to the current chroma-

togram from another chromatogram
(in the current file) and stores it in the
target curve position.

03-0014-90 • p 508
 Evaluation functions and instructions B

Instruction Description
IMPORT_PEAKTABLE Imports a peak table to the current
chromatogram from another chroma-
togram (in the current file) and stores
it in the target curve position.

PEAKTABLE_OPEN Opens the specified Peak table in the

Result file defined in File name and
stores it in the Resulting peak table. If
"*" is entered as File name the current
Result file will be used. The File name
parameter may include a path from
the current users root folder.

Export The table below contains a list of instructions for export operations.

Instruction Description
EXPORT_CURVE_AIA Exports the curve in AIA format.

EXPORT_CURVE_ASCII Exports the Source curve to the file

defined in Export to File in ASCII format.
If "*" is entered as File name the cur-
rent Result file will be used. If "?" is
entered followed by text, e.g. "Enter
a file name", as File name, a full search
path must be entered in answer to the
question. In the part of the source
curve limited by the Left limit and Right
limit every <n> sample is exported.

EXPORT_CURVE_WKS Exports the source curve to the file

defined in Export to File in WKS format.
If "*" is entered as File name the cur-
rent Result file will be used. If "?" is
entered followed by text, e.g. "Enter
a file name", as File name, a full search
path must be entered in answer to the
question. In the part of the source
curve limited by Left limit and Right
limit every <n> sample is exported

• p 509
 B Evaluation functions and instructions
B.4 Procedure instructions

Instruction Description
EXPORT_EVAL_LOG_ASCII Exports an evaluation log in ASCII
format to the file defined in Export to
file. If "*" is entered as File name the
current Result file will be used. If "?"
is entered followed by text, e.g. "Enter
a file name", as File name, a full search
path must be entered in answer to the
question.

EXPORT_EVAL_LOG_WKS Exports an evaluation log in WKS

format to the file defined in Export to
file. If "*" is entered as File name the
current Result file will be used. If "?"
is entered followed by text, e.g. "Enter
a file name", as File name, a full search
path must be entered in answer to the
question.

EXPORT_EVAL_LOG_XLS Exports an evaluation log in XLS

format to the file defined in Export to
file. If "*" is entered as File name the
current Result file will be used. If "?"
is entered followed by text, e.g. "Enter
a file name", as File name, a full search
path must be entered in answer to the
question.

EXPORT_METHOD_ASCII Exports a method to the file defined

in Export to file in ASCII format. If "*"
is entered as File name the current
Result file will be used. If all paramet-
ers are OFF then no method is expor-
ted. If Main is ON then the main meth-
od is included and if Blocks is ON then
all blocks are included in the exported
file.

03-0014-90 • p 510
 Evaluation functions and instructions B

Instruction Description
EXPORT_METHOD_WKS Exports a method to the file defined
in Export to file in WKS format. If "*"
is entered as File name the current
Result file will be used. If "?" is
entered followed by text, e.g. "Enter
a file name", as File name, a full search
path must be entered in answer to the
question. If all parameters are OFF then
no method is exported. If Main is ON
then the main method is included and
if Blocks is ON then all blocks are in-
cluded in the exported file.

EXPORT_METHOD_XLS Exports a method to the file defined

in Export to file in XLS format. If "*" is
entered as File name the current Result
file will be used. If "?" is entered fol-
lowed by text, e.g. "Enter a file
name", as File name, a full search path
must be entered in answer to the
question. If all parameters are OFF then
no method is exported. If Main is ON
then the main method is included and
if Blocks is ON then all blocks are in-
cluded in the exported file.

EXPORT_MULTI_CURVES_ASCII Exports multiple curves (previously

defined with EXPORT_SEL_CURVES in-
structions) in ASCII format to the file
defined in Export to file. If "*" is
entered as File name the current Result
file will be used. If "?" is entered fol-
lowed by text, e.g. "Enter a file
name", as File name, a full search path
must be entered in answer to the
question.

• p 511
 B Evaluation functions and instructions
B.4 Procedure instructions

Instruction Description
EXPORT_MULTI_CURVES_WKS Exports multiple curves (previously
defined with EXPORT_SEL_ CURVES
instructions) in WKS format to the file
defined in Export to file. If "*" is
entered as File name the current Result
file will be used. If "?" is entered fol-
lowed by text, e.g. "Enter a file
name", as File name, a full search path
must be entered in answer to the
question.

EXPORT_MULTI_CURVES_XLS Exports multiple curves (previously

defined with EXPORT_SEL_ CURVES
instructions) in XLS format to the file
defined in Export to file. If "*" is
entered as File name the current Result
file will be used. If "?" is entered fol-
lowed by text, e.g. "Enter a file
name", as File name, a full search path
must be entered in answer to the
question.

EXPORT_NORMALISE_RETENTION Normalizes retention when exporting

multiple curves.

EXPORT_PEAKTABLE_ASCII Exports the peak table in Peak table

source to the file defined in Export to
file in ASCII format. If "*" is entered as
File name the current Result file will
be used. If "?" is entered followed by
text, e.g. "Enter a file name", as File
name, a full search path must be
entered in answer to the question.

EXPORT_PEAKTABLE_WKS Exports the peak table in Peak table

source to the file defined in Export to
file in WKS format. If "*" is entered as
File name the current Result file will
be used. If "?" is entered followed by
text, e.g. "Enter a file name", as File
name, a full search path must be
entered in answer to the question.

03-0014-90 • p 512
 Evaluation functions and instructions B

Instruction Description
EXPORT_PEAKTABLE_XLS Exports the peak table in Peak table
source to the file defined in Export to
file in XLS format. If "*" is entered as
File name the current Result file will
be used. If "?" is entered followed by
text, e.g. "Enter a file name", as File
name, a full search path must be
entered in answer to the question.

EXPORT_PEAKTABLE_XML Exports the peak table in Peak table

source to the file defined in Export to
file in XML format. If "*" is entered as
File name the current Result file will
be used. If "?" is entered followed by
text, e.g. "Enter a file name", as File
name, a full search path must be
entered in answer to the question.

EXPORT_SEL_CURVES Selects a curve for subsequent export

(using the EXPORT_MULTI-CURVES_*
instruction). The curve is cut according
to the right and left cut limit and the
number of points to be exported may
be set by the Export parameter (for ex-
ample, every fifth point).

EXPORT_DOC_400_ASCII Exports the documentation in the

current result file in ASCII format to
the file defined in Export to file. If "*"
is entered as File name the current
Result file will be used. If "?" is
entered followed by text, e.g. "Enter
a file name", as File name, a full search
path must be entered in answer to the
question. If all parameters to this
function are OFF then no documenta-
tion is exported. If at least one of them
is ON then the documentation will be
exported and the corresponding parts
will be included in the exported file.

• p 513
 B Evaluation functions and instructions
B.4 Procedure instructions

Instruction Description
EXPORT_DOC_400_WKS Exports the documentation in the
current result file in WKS format to
the file defined in Export to file. If "*"
is entered as File name the current
Result file will be used. If "?" is
entered followed by text, e.g. "Enter
a file name", as File name, a full search
path must be entered in answer to the
question. If all parameters to this
function are OFF then no documenta-
tion is exported. If at least one of them
is ON then the documentation will be
exported and the corresponding parts
will be included in the exported file.

EXPORT_DOC_400_XLS Exports the documentation in the

current result file in MS Excel XLS
format to the file defined in Export to
file. If "*" is entered as File name the
current Result file will be used. If "?"
is entered followed by text, e.g. "Enter
a file name", as File name, a full search
path must be entered in answer to the
question. If all parameters to this
function are OFF then no documenta-
tion is exported. If at least one of them
is ON then the documentation will be
exported and the corresponding parts
will be included in the exported file.

EXPORT_DOC_WKS Exports the documentation in the

current result file in WKS format to
the file defined in Export to file. If "*"
is entered as File name the current
Result file will be used. If "?" is
entered followed by text, e.g. "Enter
a file name", as File name, a full search
path must be entered in answer to the
question. If all parameters to this
function are OFF then no documenta-
tion is exported. If at least one of them
is ON then the documentation will be
exported and the corresponding parts
will be included in the exported file.

03-0014-90 • p 514
 Evaluation functions and instructions B

Instruction Description
EXPORT_DOC_XLS Exports the documentation in the
current result file in XLS format to the
file defined in Export to file. If "*" is
entered as File name the current Result
file will be used. If "?" is entered fol-
lowed by text, e.g. "Enter a file
name", as File name, a full search path
must be entered in answer to the
question. If all parameters to this
function are OFF then no documenta-
tion is exported. If at least one of them
is ON then the documentation will be
exported and the corresponding parts
will be included in the exported file

EXPORT_DOC_ASCII Exports the documentation in the

current result file in ASCII format to
the file defined in Export to file. If "*"
is entered as File name the current
Result file will be used. If "?" is
entered followed by text, e.g. "Enter
a file name", as File name, a full search
path must be entered in answer to the
question. If all parameters to this
function are OFF then no documenta-
tion is exported. If at least one of them
is ON then the documentation will be
exported and the corresponding parts
will be included in the exported file.

Chromatogram The table below contains a list of instructions for chromatogram functions.
functions
Instruction Description
COPY_CHROM Creates a copy of the specified chroma-
togram. If "*" is used as source then
the current (default) chromatogram is
used. If "*" is used as destination then
a default name will be created for the
copy.

• p 515
 B Evaluation functions and instructions
B.4 Procedure instructions

Instruction Description
CREATE_NEW_CHROM Creates a new chromatogram with the
given name. If "*" is used for the
chromatogram name a default name
will be generated and used.
Note: It is a recommendation not to
use only numbers as names for new
chromatograms.

DELETE_CHROM Deletes the named chromatogram. If

trying to delete the current (default)
chromatogram a run time error will
be caused.

OPEN_CHROM Opens the specified chromatogram

from the specified file.

RENAME_CHROM Renames the specified chromatogram.

If "*" is used as From then the current
(default) chromatogram is used.

RESTORE_DESTINATION_ CHROM Resets the destination for the sub-

sequent curve and peak table opera-
tions to the default chromatogram.
Used in pair with the SET_DESTINA-
TION_CHROM instruction.

SET_DESTINATION_CHROM Opens the named chromatogram as

destination for the subsequent curve
and peak operations. Used in pair with
the RESTORE_ DESTINATION_CHROM
instruction.

Other instructions The table below contains a list of instructions for other operations.

Instruction Description
BASE Sets the X-axis base that the following
calculations will be made in. If the
value of the X-axis base is DEFAULT,
then the default base is used (usually
the base the method was run in). This
instruction should be the first in the
evaluation procedure, otherwise it will
have no effect at all.

03-0014-90 • p 516
 Evaluation functions and instructions B

Instruction Description
Comment Inserts a comment below the marked
instruction.

ENDLOOP Marks the end of a LOOP statement.

LOOP The instructions between this state-

ment and the ENDLOOP statement are
repeated n times. It is possible to have
loops within loops as long as the
number of LOOP statements matches
the number of ENDLOOP statements.

MOLSIZE Calculates the molecular sizes from a

molecular size curve.
A Mol. size column will be added to
the Peak table.

QUANTITATE Calculates the concentration and

amounts in the sample from a quantit-
ation table.
Amount and Concentration columns will
be added to the Peak table.

REPORT Prints a report with the specified

named report layout and title. If Title
is "*" then the title in the report lay-
out is used. If Report Layout is "*" then
a default layout is used.

RUN_PROGRAM Starts a program as a separate process.

The Program name string contains the
program name and parameters to start
it with.

UPDATE Updates a Quantitation table with new

data from one standard concentration
level.
The default Limit(+/-) value of 12.5%
will be used.

• p 517
 B Evaluation functions and instructions
B.4 Procedure instructions

Test instructions The Instruction field also contains a group of test instructions. These instructions
are only available for the UNICORN software development team.

Instruction Description
AUTOSAMPLER_PEAK_INTERVALS Sets the area intervals for the AUTO-
SAMPLER_PEAK_TEST.

AUTOSAMPLER_PEAK_TEST Locates the first peak in the peak

table. Compares the area of the peak
in the peak table with the specified
maximum and minimum areas.

GRADIENT_TEST_INTERVALS Sets the level intervals for the GRADI-

ENT_TEST.

GRADIENT_TEST The theoretical straight line between

the 0% and 100% levels are calcu-
lated. The deviation between the curve
and the ideal straight line is compared
in both directions from the center pos-
ition (50%) until the deviation exceeds
the defined maximum deviation. The
calculated deviation points arechecked
against the defined limits.

STEP_RESPONSE_INTERVALS Sets the level intervals for the

STEP_RESPONSE_TEST.

STEP_RESPONSE_TEST The relative amplitude is calculated at

the specified retentions (The 0% and
100% amplitudes are used for refer-
ence). The calculated relative amp-
litudes are checked against the spe-
cified error margins. The 0% level
amplitude is verified to be within the
specified interval from the absolute 0
level.

UV_RESPONSE_INTERVALS Sets the level intervals for the UV_RE-

SPONSE_TEST.

03-0014-90 • p 518
 Evaluation functions and instructions B

Instruction Description
UV_RESPONSE_TEST The amplitudes for the 0% and 100%
levels are calculated and the difference
between the values are calculated. The
results of (1) Curve2_Difference /
Curve1_Difference and (2)
Curve2_Difference / Curve3_Differ-
ence are calculated. The calculated
points are checked if they are outside
the defined limits from the 50% level.

• p 519
 C Curve fit models and statistics

C Curve fit models and statistics

Introduction The Analysis module (optional) is used to produce calibration curves and molecular
size curves for analytical purposes. The quality of the curve fit model determines
the accuracy of the curves. This appendix describes
• The available curve fit models.
• The statistical measurements in the Analysis module.

In this appendix This appendix contains these sections:

Topic See
Curve fit models C.1

Statistics C.2

03-0014-90 • p 520
 Curve fit models and statistics C

C.1 Curve fit models

Calibration curve The Analysis module provides a comprehensive range of curve fit models. The
models following models are available for calibration curves:
• Linear.
• Linear through origin.
• Quadratic.
• Quadratic through origin.
• Point to point.

Note: The average peak size for all points at a specific level is used to calculate the
calibration curve.

Molecular size The following curve fit models are available for molecular size curves:
curve models
• Linear.
• Linear (log Mw).
• Quadratic.
• Quadratic (log Mw).
• Point to point.
• Point to point (log Mw).

Statistics The Analysis module provides values for the appropriate constants that are used
in each curve equation for all models, except for the point to point models. It also
provides statistical data that you can use to assess the quality of fit of the curve to
the data.
• Click the More... button in the Statistics field of the Quantitation table or Mol.
size table dialog boxes to view the applied model statistics.

The Linear model The table below describes the features of the Linear curve fit model.

Feature Description
Equation. y = Ax + B

Mathematical model. The constants A and B are determined

by linear least squares regression.

Minimum number of required points. 2 (at least 4 points recommended)

Measuring range for the calibration Within the highest and lowest values
curve. for the points.

• p 521
 C Curve fit models and statistics
C.1 Curve fit models

Note: A variant of this model is available for the production of a molecular size
curve. This uses the logarithm of the molecular size as the x value in the expression
above.
The illustration below is an example of the statistical information for an applied
Linear curve model:

The Linear The table below describes the features of the Linear through origin curve fit model:
through origin
model
Feature Description
Equation. y = Ax

Mathematical model. The constant A is determined by linear

least squares regression.

Minimum number of required points. 1 (at least 2 points recommended)

Measuring range for the calibration From the point with the highest value
curve. down to the origin.

The illustration below is an example of the statistical information for an applied

Linear through origin curve model:

03-0014-90 • p 522
 Curve fit models and statistics C

The Quadratic The table below describes the features of the Quadratic curve fit model:
model
Feature Description
Equation. y = Ax2 + Bx + C

Mathematical model. The constants A, B and C are determ-

ined by linear least squares regression.

Minimum number of required points. 3 (at least 6 points recommended)

Measuring range for the calibration Within the highest and lowest values
curve. for the points.

Note: A variant of this model is available for the production of a molecular size
curve. This uses the logarithm of the molecular size as the x value in the expression
above.
The illustration below is an example of the statistical information for an applied
Quadratic curve model:

The Quadratic The table below describes the features of the Quadratic through origin curve fit
through origin model:
model

Feature Description
Equation. y = Ax2 + Bx

Mathematical model. The constants A and B are determined

by linear least squares regression.

Minimum number of required points. 2 (at least 4 points recommended)

Measuring range for the calibration From the point with the highest value
curve. down to the origin.

• p 523
 C Curve fit models and statistics
C.1 Curve fit models

The illustration below is an example of the statistical information for an applied

Quadratic through origin curve model:

The Point to point The table below describes the features of the Point to point curve model.
model
Feature Description
Equation. No single equation.

Mathematical model. As these curves are not based on a

single equation, no statistical data is
available. The statistics table only
contains information on the number
of points in the curve.

Minimum number of required points. 2

Measuring range for the calibration Within the highest and lowest values
curve. for the points.

The illustration below is an example of the statistical information for an applied

Point to point curve model:

03-0014-90 • p 524
 Curve fit models and statistics C

C.2 Statistics

Introduction This section explains the correlation and explained variance calculations that are
used by the Analysis module.

Correlation The Analysis module calculates the correlation coefficient for linear models. This
shows how well the data are linearly related. The correlation is displayed in the
Statistics table.
If you are producing a calibration curve that relates peak area or height to amount
or concentration, you aim to achieve a high positive correlation coefficient. A value
of +1 indicates a perfect fit of all the data to the straight line. A molecular size
curve has a negative slope, so the aim is towards a correlation coefficient of -1.

Too few data If you only have two data points for a Linear model, or only one point for a Linear
points through origin model, the fitted straight line will inevitably pass exactly through
the points. By definition, this leads to a correlation of exactly +1, but this does not
indicate a good fit, but instead indicates too few data points. In these cases the
Statistics table will display a “---” symbol instead of the correlation value.

Correlation calcu- The correlation is derived as follows:

lation

Explained vari- Explained variance provides a measurement of how much of the variation in the
ance data points (xy pairs) is due to the model. The remaining variation can be attributed
to noise, i.e. random errors, or to the fact that an inappropriate model has been
selected. This makes it possible to use the explained variance value for model
selection, e.g. to decide if a quadratic model fits the data better than a linear model.
This would be confirmed by a higher explained variance value.

• p 525
 C Curve fit models and statistics
C.2 Statistics

Note: The explained variance is not calculated for curve models drawn through
the origin.

Explained vari-
ance calculation The explained variance is equal to R2 adjusted for degrees of freedom. The
illustration below shows the mathematical model:

Undefined value You can only obtain a value for explained variance if you have sufficient data
for explained vari- points on the curve. For instance, if you only have two points for a Linear model,
ance
or only three points for a Quadratic model, the fitted curve will pass exactly through
the points. By definition, this leads to an undefined value for explained variance.
In these cases the Statistics table will show a “---” symbol instead of an explained
variance value.

03-0014-90 • p 526
 The Column list D

D The Column list

Introduction The Column List includes all available columns and their specific parameters. This
appendix describes how to edit the Column List.

In this appendix This appendix contains this section:

Topic See
How to edit the Column List D.1

• p 527
 D The Column list
D.1 How to edit the Column List

D.1 How to edit the Column List

Introduction This section describes how to edit the list of available columns.

Available columns When you create a new method and select a column, certain column-specific
parameters are automatically copied into the method. The list of available columns
is found in the For column field of the New Method dialog box. The Column List is
not linked to a particular method, although the columns are edited within the
Method Editor.
Columns are either globally available to all users, or only personally available. It
is best not to edit the globally available columns, unless you save the changes under
a new column name, since other users may not appreciate the changes.
Note: It is recommended that only a limited number of users are given access to
the right to edit global columns. This is essential to avoid unintentional changes.

How to print the The table below describes how to print the column list data.
column list
Step Action
1 • Click the print button.

Result: The Print Column List dialog box opens.

2 • Select to print a global or your personal column list.

• Click OK.

Result: The column list is printed on the default Windows printer.

03-0014-90 • p 528
 The Column list D

The New Column The illustration below shows the New Column dialog box:
dialog box

How to add a new The table below describes how to add a new column to the Column List.
column
Step Action
1 • Choose Edit:Column List in the Method Editor.

Result: The Column List dialog box opens.

Note: Select a column from the list to display the parameters in the
field to the right. Most column parameters are displayed in the Nor-
mal Parameters tab. Additional parameters for special columns may
be displayed in the Advanced Parameters tab.

2 • Click the New button.

Result: The New Column dialog box opens.

3 • Select the appropriate parameter tab.

• Type the desired parameter values.
• Click the Save as button.

Note: Mandatory parameters are labelled mand. The column cannot

be saved unless all mandatory parameters are filled in.
Result: The Save as dialog box opens.

• p 529
 D The Column list
D.1 How to edit the Column List

Step Action
4 • Type the name of the new column.
• Click the Save as global checkbox if the column should be avail-
able to other users.

Note: You must have Edit global lists authorization to save a column
for global use. A global column cannot have the same name as a
personal column.
• Click OK.

Result: The new column is added to the Column List.

Note: See column instruction to determine the back pressure over the system and
the column.

The normal The table below is a list of all the available normal column parameters:
column paramet-
ers
Parameter Unit Comment
Height cm • Mandatory.
• Calculation of N/m.

Diameter cm • Mandatory.

Column volume nl, µl, ml or liter • Mandatory.

• Automatically calcu-
lated from Height and
Diameter.
• User cannot set this
parameter directly.

Column volume unit nl, µl, ml or liter • Not mandatory.

• The column volume
is calculated in the set
unit.

Technique • Mandatory.
• Decides which tech-
nique the column
should be available
for.

03-0014-90 • p 530
 The Column list D

Parameter Unit Comment

Vt nl, µl, ml or liter • Not mandatory.
• Total liquid volume.
• Used to calculate the
capacity factor after
an integration.

Vo nl, µl, ml or liter • Not mandatory.

• Void volume.
• Used to calculate Kav
after integration.

Max pressure MPa • Mandatory.

• Used for setting pres-
sure limit in a meth-
od automatically.

Default flowrate nl/min, µl/min, ml/min • Mandatory.

or liter/min • Used to set the
flowrate in a method
automatically.

Max flowrate nl/min, µl/min, ml/min • Not mandatory.

or liter/min • Used to give a warn-
ing if a higher
flowrate is chosen
when saving or start-
ing a method.

Typ. peak width at base nl, µl, ml or liter • Not mandatory.

• Used to set averaging
time for UV detector.
• used to set peak frac-
tionation parameters.

pH high value, longterm • Not mandatory.

• Used to give a warn-
ing when saving or
starting the method
if the BufferPrep_pH
value is higher than
the set value.

• p 531
 D The Column list
D.1 How to edit the Column List

Parameter Unit Comment

pH low value, longterm • Not mandatory.
• Used to give a warn-
ing when saving or
starting the method
if the BufferPrep_pH
value is lower than
the set value.

pH high value, shortterm • Not mandatory.

• Used to give a warn-
ing when saving or
starting the method
if the BufferPrep_pH
value is higher than
the set value.

pH low value, shortterm • Not mandatory.

• Used to give a warn-
ing when saving or
starting the method
if the BufferPrep_pH
value is lower than
the set value.

Average particle diameter µm • Not mandatory.

• Information only.

Code no • Not mandatory.

• Information only.

Typical loading range mg • Not mandatory.

• Information only.

Mol. weight range kDa • Not mandatory

• Information only

Scan rate spectra/sec • Not mandatory.

• Information only.

Note: The values for the parameters Max pressure, Default flowrate and Typical peak
width at base (used to set average time and peak fractionation parameter MinWidth)
are only copied into the method if the corresponding instructions are available as
variables.

03-0014-90 • p 532
 The Column list D

How to edit The table below describes how to edit column parameters in the Method Editor:
column paramet-
ers
Step Action
1 Choose Edit:Column List.
Result: The Column List dialog box opens.

2 Select a column and click the Edit button.

Result: The Edit Column dialog box opens.

3 Select the desired parameters and change the value settings.

4 • Click the Save button.

or
• Click the Save as button to save the column under a new name.

Note: If a column has been selected and saved in a method, and the parameters
for the column are changed later, the column in the method will not be updated
automatically. When you open the method you will be asked if you want to update
the parameters. The recommendation is that you answer Yes.

How to delete a The table below describes how to delete a column:

column.
Step Action
1 Choose Edit:Column List.
Result: The Column List dialog box opens.

2 Select a column and click the Delete button.

Result: The Delete Column dialog box opens.

3 • Click the checkbox for each column you want to delete.

• Click OK.

Result: The selected columns are deleted.

How to export a The column information for a system can be transferred to another by using the
column. export and import functions in the column list. The table below describes how to
export a column:

Step Action
1 Choose Edit:Column List.
Result: The Column List dialog box opens.

• p 533
 D The Column list
D.1 How to edit the Column List

Step Action
2 Click the Export button.
Result: The Export Column dialog box opens.

3 • Click the checkbox for each column you want to export.

• Click Export.

Result: The Export Column to file dialog box opens.

4 • Select the desired folder in the navigation window.

• Type a new file name if neccessary.
• Choose the type of file to export (column file or Excel file)
• Click the Save button.

Result: The column file is saved and the dialog box closes.

Note: If a column is selected in the Column List when the Export Column dialog box
is opened, this column will automatically be selected in the Export Column dialog
box.

How to import a The table below describes how to import a column:

column.
Step Action
1 Choose Edit:Column List.
Result: The Column List dialog box opens.

2 Click the Import button.

Result: The Import Column dialog box opens.

3 • Click the Browse button to locate the column file.

Result: The Import Column from file dialog box opens.

4 • Select a column file.

• Click Open.

Result: The Import Columns dialog box opens.

5 • Select the columns to import from the list.

• Select Import as global to add the columns to the global column
list if desired.
• Click Import.

Result: The selected columns are imported and available in the

column list.

03-0014-90 • p 534
 The Column list D

Note: Select Import as global to import the columns to the global column list.

• p 535
 E How to create and edit BufferPrep recipes

E How to create and edit BufferPrep recipes

Introduction The BufferPrep function is available for some ÄKTAdesign systems. This appendix
describes how to create and how to edit the recipes for BufferPrep.

In this appendix This appendix contains these sections:

Topic See
How to create a BufferPrep recipe E.1

How to edit a BufferPrep recipe E.2

03-0014-90 • p 536
 How to create and edit BufferPrep recipes E

E.1 How to create a BufferPrep recipe

About BufferPrep New BufferPrep recipes are created in the Method Editor. The list of recipes is not
recipes linked to a specific method. Which recipe to use in a certain method is selected on
the BufferPrep tab in the Run Setup.

How to create a The table below describes how to create a new BufferPrep recipe in the Method
recipe Editor:

Step Action
1 Choose Edit:BufferPrep Recipes.
Result: The BufferPrep Recipes dialog box opens.
The illustration below shows the BufferPrep Recipes dialog box with
a recipe selected:

2 Click the New button.

Result: The New Recipe dialog box opens.
The illustration below shows a complete example of a BufferPrep
recipe in the New Recipe dialog box.

• p 537
 E How to create and edit BufferPrep recipes
E.1 How to create a BufferPrep recipe

Step Action
3 Select buffers from the Buffer substances droplists and type stock
concentrations in the corresponding Stock conc box.
See “How to define a new buffer substance” below if the desired
substance is not available.

4 Select either HCl (acid) or NaOH (base) from the Acid/Base droplist
and type the required stock concentration (typically 0.1 M)

5 Select a salt from the Salt droplist and type the maximum outlet
concentration of the salt for 100%B (typically 1.0 M).
See “How to define a new salt” below if the desired salt is not
available.

6 Type the desired pH range minimum and maximum values in the

From and To boxes.
See “How to select the pH range” below this table.

7 • Click the Notes button (optional).

• Type your notes about the recipe in the displayed dialog box.
• Click OK to return to the New Recipe dialog box.

8 Click Save as to save the recipe under a new name.

Note: A warning message will appear if any of the recipe values are
unfeasible.

9 • Type a name in the dialog box.

• Click OK.

Result: The new recipe is added to the recipe list.

Note: It is recommended that restricted access be given to the right to edit global
recipes.
The recipes are either globally available to all users, or only personally available.
It is best not to edit the globally available recipes, unless you save the changes
under a new recipe name, since other users may not appreciate the changes.

Buffer concentra- Use buffer concentrations that are 2-4 times higher than the concentration that is
tion used in the normal preparation. When BufferPrep is used, the buffer will be diluted
2-10 times depending on the amount of acid/base that has to be used to reach the
desired pH value.
Up to five different buffering components can be selected. To prevent a too high
ionic strength, the sum of the concentrations for all selected buffers should be
between 0.03 M and 0.2 M (typically 0.1 M).

03-0014-90 • p 538
 How to create and edit BufferPrep recipes E

How to select the The useful pH range depends on the pKa value. The table below describes how to
pH range determine a pH range based on the pKa value:

Step Action
1 Choose Edit:BufferPrep Recipes and click the New button.

2 Click the Buffer substance button in the New Recipe dialog box.
Result: The Define buffer substance dialog box opens.

3 Select the buffer component from the Name droplist and note the
displayed pKa value.

4 Click Cancel to return to the New Recipe dialog box.

5 Use the pKa value to determine the pH range. Typically a range of

+/- 0.5 units around the pKa value is useful.
Note: Check buffer tables for the exact ranges.

How to define a Note: Before you can define a new buffer substance you must ensure that all pKa
new buffer sub- values are available for the substance. The pKa values should be true (i.e. the pKa
stance
value at indefinite dilution) and not apparent pKa values (i.e. measured at a non-zero
concentration). The pKa values should be given at 25° C.
The table below describes how to define a new buffer substance:

Step Action
1 Choose Edit:BufferPrep Recipes and click the New button.

2 Click the Buffer substance button in the New Recipe dialog box.
Result: The Define buffer substance dialog box opens.

• p 539
 E How to create and edit BufferPrep recipes
E.1 How to create a BufferPrep recipe

Step Action
3 Click the New button.
Result: The New component dialog box opens.

4 Type a name for the new component and click OK to return to the
Define buffer substance dialog box.

5 Type appropriate values in the Value cells for each pKa and dpKa/dT
parameter.
Note: All values must fall within the stated Range limits. Up to three
values can be entered for each buffering component. When the
component has less than three pKa values, the other values should
be set to zero. A dpKa/dT value of zero means that the pKa does
not change with temperature.

6 • Type the number of acidic protons for the buffer substance in the
form that it is actually weighed in.

Example: The number is 2 for NaH2PO4, 1 for Na2HPO4 and 0 for

Tris.
• Type the charge of the completely de-protonated ion. This will
be a negative value for an acid and zero for a base.

Example: The value is -3 for NaH2PO4 and 0 for Tris.

• Click OK.

Result: The new buffer substance is added to the list of available

substances.

How to define a Before you can define a new salt you must ensure that the new salt is inert, i.e. a
new salt salt with no buffering properties. The table below describes how to define a new
salt:

Step Action
1 Choose Edit:BufferPrep Recipes and click the New button.

03-0014-90 • p 540
 How to create and edit BufferPrep recipes E

Step Action
2 Click the Salt button in the New Recipe dialog box.
Result: The Define salt dialog box opens.

3 Click the New button.

Result: The New component dialog box opens.

4 Type a name for the new salt and click OK to return to the Define
salt dialog box.

5 • Type the appropriate charge of anion value in the corresponding

Value cell.

Example: The value for Cl- is - 1. The value for SO42- is -2.
• Type the appropriate charge of the cation value in the correspond-
ing Value cell.

Example: The value for Na+ is 1. The value for Mg2+ is 2.

• Click OK.

Result: The new salt is added to the list of available salts.

• p 541
 E How to create and edit BufferPrep recipes
E.2 How to edit a BufferPrep recipe

E.2 How to edit a BufferPrep recipe

Introduction This section describes how to edit a BufferPrep recipe in the Method Editor.

How to edit a re- The table below describes how to edit a BufferPrep recipe:
cipe
Step Action
1 Choose Edit:BufferPrep Recipes.
Result: The BufferPrep Recipes dialog box opens.

2 Select a recipe and click the Edit button.

Result: The Edit Recipe dialog box opens.

3 Change the substances and parameters as desired and click the Save
button or the Save as button to save the new recipe.

Changes to recipes If a recipe has been selected and saved in a method, and the recipe is later changed,
in methods the corresponding recipe in the method will not be updated automatically. When
you open the method you will be asked if you want to update the parameters in
the method recipe. The recommendation is that you answer Yes.
Note: The question will not appear if you only change the Correction factors. The
Correction factors in the method recipe will not be updated.

How to determine Correction factors can be set to fine-tune a recipe around a specific pH, to obtain
if the Correction high pH accuracy. The table below describes how to run the BufferPrep manually
factors need to be
changed at 0% and 100% in the System Control module, to determine if the Correction factors
need to be changed:

Step Action
1 Choose Manual:Other.
Result: The System Other instructions dialog box opens.

03-0014-90 • p 542
 How to create and edit BufferPrep recipes E

Step Action
2 • Select the recipe from the Recipe Name droplist and click the Ex-
ecute button.

Result: The recipe instruction is added.

3 • Click the Pump radio button and select BufferPrep_pH.

• Set the pH value in the pH parameter box and click the Execute
button.

Result: The BufferPrep pH value is added and the run starts.

4 • Select Flow.
• Set the flow rate in the FlowRate parameter box and click Execute.

Result: The new flow rate is added.

5 Check the pH reading when it is stable in the BuffPre_pH meter in

the Run Data pane.
Note: At least 30 ml of eluent must pass through before the reading
stabilizes.
To display the BuffPre_pH meter, right-click and select Properties.
Select BuffPre_pH on the Run Data Groups panel and click the OK
button.

6 • Select Gradient in the Instructions list.

• Type 100% in the Target parameter box, 0 in the Length parameter
box and click Execute.

Result: The gradient instruction is added.

7 Check the pH reading when it is stable at 100%.

See “How to change the Correction factors” below.

How to change If the readings described in the instruction above are acceptable at both 0% and
the Correction 100%, the Correction factors do not need to be changed. If the Correction factors
factors
do not produce an acceptable result, they must be adjusted in the Method Editor
module. The table below describes how to change the Correction factors:

Step Action
1 Choose Edit:BufferPrep Recipes.
Result: The BufferPrep Recipes dialog box opens.

2 Select the recipe from the Recipe droplist and click the Edit button.
Result: The Edit Recipe dialog box opens.

• p 543
 E How to create and edit BufferPrep recipes
E.2 How to edit a BufferPrep recipe

Step Action
3 Click the Correction factors button.
Result: The Correction Factors dialog box opens.

4 • Type the deviation at 0% and 100%.

Example: If the pH is set to 7.0 and the actual pH is 7.1, the Correc-
tion factor is 0.1. If the actual pH is 6.9, the Correction factor is -0.1.
• Click OK.
• Click the Save button or the Save as button to save the recipe.

Note: If there already are Correction factors, the measured pH deviation should be
added to the old factors.

03-0014-90 • p 544
 Method examples F

F Method examples

Introduction This appendix contains practical method examples that can be applied in typical
situations. The examples cover three different topic groups:
• Watch instructions
• Messages
• Quality control

Watch instructions allow the progress of a method run to be determined by the

events during the method run, for example start collecting fractions when the first
peak elutes, or equilibrate the column until the eluent conductivity has reached a
given value. This is facilitated by the Watch instructions.
The system strategy includes Watch instructions for each monitor defined in the
system. These instructions are used to survey method runs, and instruct the system
to call a specified block or an instruction when a particular monitor signal meets
a given condition. As long as the condition is not met, the block is not activated.
Messages can be used in a method to provide information to the operator but also
for interaction between the system and the operator.
A Quality control procedure in a method can be used to ensure that the quality of
the results remain consistent in a series of runs.

In this appendix This appendix contains the following sections:

Topic See
Simple equilibration F.1

Equilibration with simple safeguard F.2

Equilibration with extra safeguard F.3

Collection of absorbance peaks F.4

Collection of three absorbance peaks F.5

Messages F.6

Quality control procedure F.7

• p 545
 F Method examples
F.1 Simple equilibration

F.1 Simple equilibration

Introduction This section contains an example of how a Watch instruction for simple equilibration
can be inserted into a method.

Example instruc- This is an example instruction as it would be presented in the Text pane.
tion
0.00 Block EQUILIBRATE
(Equilibrate)
0.00 Base SameAsMain
0.00 Watch_Cond Less_than, 5 {mS/cm}, CONTINUE
0.00 Hold
0.10 Watch_UV1 Less_than, 100 {mAU}, CONTINUE
0.10 Hold
0.10 End_Block
If you are not using ÄKTA instruments
If you are not using ÄKTA instruments, a delay should be added after the Hold/Pause
instruction so that the following instruction will not be executed simultaneously
with the Hold/Pause instruction.

This is what hap- The table below describes what happens in the above example:
pens
Stage Description
1 The Watch is started on the conductivity signal and the method is
then put on Hold.

2 Continue is issued and Watch_cond is turned off automatically when

the Watch_cond condition is fulfilled.

3 Method execution continues issuing a Watch_UV command. Again

the method is put on Hold until the Watch condition is fulfilled.

Note: Even though the line

Watch_Cond Less_than, 5 {mS/cm}, Continue
is in the method placed before Hold, the method is put on hold first and then
continued only after the conductivity has reached a level less than 5 mS/cm. This
is because Hold is an instruction that will be executed at its breakpoint, while
Continue is not an instruction but rather an action for the Watch instruction.

03-0014-90 • p 546
 Method examples F

Evaluation of the This method works satisfactorily although one drawback is that it might never
method end, and thus consume all of the buffer if the conditions for some reason are
unfulfilled. See appendices F.2 Equilibration with simple safeguard on page 548 and
F.3 Equilibration with extra safeguard on page 549.

• p 547
 F Method examples
F.2 Equilibration with simple safeguard

F.2 Equilibration with simple safeguard

Introduction This section contains an example of how a Watch instruction for simple safeguard
can be inserted into a method.

Example instruc- This is an example instruction as it would be presented in the Text pane:
tion
0.00 Block EQUILIBRATE
(Equilibrate)
0.00 Base SameAsMain
0.00 Watch_UV1 Less_than, 100 {mAU} END_BLOCK
5.00 Watch_Off UV1
5.00 Message “The Condition was never reached”, Screen,
“No sound”
5.00 End_Block

This is what hap- This is what happens in the above example:

pens
The column is equilibrated until the UV has reached a level below 100 mAU or
until the column has been equilibrated with five column volumes of buffer,
whichever condition is met first. In this way, it is possible to equilibrate the column
without the risk of running out of buffers.

03-0014-90 • p 548
 Method examples F

F.3 Equilibration with extra safeguard

Introduction This section contains an example of how a Watch instruction for extra safeguard
can be inserted into a method.

Example instruc- This is an example instruction as it would be presented in the Text pane:
tion
0.00 Block EQUILIBRATE
(Equilibrate)
0.00 Base SameAsMain
0.00 Block COND_LESS_THAN
(Cond_less_than)
0.00 Base SameAsMain
0.00 Watch_Cond Less_than, 5 {mS/cm} END_BLOCK
6.00 Message “Low conductivity not reached”, Screen,
“No sound”
6.00 Pause INFINITE {Minutes}
6.00 End_Block
0.00 Block COND_STABLE
(Cond_stable)
0.00 Base SameAsMain
0.00 WatchPar_Cond 0.500 {mS/cm}, 2 {mS/cm}
0.00 Watch_Cond Stable_Baseline, 5 {Minutes},
END_BLOCK
10.00 Message “Conductivity not stable”, Screen,
“No sound”
10.00 Pause INFINITE {Minutes}
10.00 End_Block
0.00 End_Block
Note: If you are not using ÄKTA instruments, a delay should be added after the
Hold/Pause instruction so that the following instruction will not be executed
simultaneously with the Hold/Pause instruction.

This is what hap- The table below describes what happens in the above example:
pens
Stage Description
1 The column is equilibrated until the conductivity is below 5 mS/cm.

2 If this value is not reached within 6 column volumes, the method is

paused and a message is displayed.

• p 549
 F Method examples
F.3 Equilibration with extra safeguard

Stage Description
3 Equilibration of the column is continued until the conductivity value
is “stable” (allowed to vary by max. ±2 mS/cm) over a period of at
least 5 minutes.

4 If this condition is not met within 10 column volumes, the method

is again paused.

Note: At each pause, the operator can decide whether to continue or abort the run.

03-0014-90 • p 550
 Method examples F

F.4 Collection of absorbance peaks

Introduction This section contains an example of how to collect absorbance peaks through
outlets F3 and F4.

Example instruc- This is an example instruction as it would be presented in the Text window:
tion
0.00 Block ELUTION
(Elution)
0.00 Base SameAsMain
0.00 Gradient 100.0 {%}, 20.00 {base}
0.00 Watch_UV1 Greater_Than, 100 {mAU}, Peak_1
(Peak_1)
0.00 Base SameAsMain
0.00 OutletValve F3
0.00 Watch_UV1 Less_Than_Or_Valley, 100 {mAU}, Waste
(Waste)
0.00 Base SameAsMain
0.00 OutletValve WasteF1
0.00 Watch_UV1 Greater_Than, 100 {mAU}, Peak_2
(Peak_2)
0.00 Base SameAsMain
0.00 OutletValve F4
0.00 Watch_UV1 Less_Than, 100 {mAU}, End_collect
(End_collect)
0.00 Base SameAsMain
0.00 OutletValve WasteF1
0.00 End_Block
0.00 End_Block
0.00 End_block
0.00 End_Block
20.00 End_Block

• p 551
 F Method examples
F.4 Collection of absorbance peaks

Illustration The illustration below shows peaks collected by the method in the example above.

This is what hap- In this example, one or two absorbance peaks are collected through outlets F3 and
pens F4 respectively with waste fractions collected through outlet valve F1 (waste). Each
called block (except End_collect) resets the Watch condition so that the method
reacts correctly to subsequent changes in the UV absorbance.

Invalid Watch in- The design of a method of this kind (with several Watch instructions for the same
structions monitor) is important. The construction in the following three lines appears simpler
but is incorrect:
0.00 Watch_UV Greater_than, 100 {mAU}, Peak_2
0.00 Watch_UV Less_than, 100 {mAU}, End_collect
0.00 End_block
Here, the second Watch instruction will annul the first, since a signal can only be
watched for one condition at a time.

03-0014-90 • p 552
 Method examples F

F.5 Collection of three absorbance peaks

Introduction This section contains an example of how to collect three absorbance peaks through
outlets F3, F5 and F7 with waste fractions through outlets F4, F6 and F8.
The maximum number of peaks collected in this example is three due to the limited
number of positions on the outlet valve.

Recommendations Waste container needed

The waste fractions between the peaks are collected through the outlet valve
positions F4, F6 and F8, so ensure that the tubing from these positions is lead to
a suitably large container.
Condition for UV threshold
The UV threshold for collecting the waste fraction must be below the threshold
for collecting the peak fraction so that the “waste” condition will not be fulfilled
simultaneously or immediately after peak collection.

Example instruc- This is an example instruction as it would be presented in the Text window:
tion
0.00 Block Eluate_Fractionation
(Eluate_Fractionation)
0.00 Base SameAsMain
0.00 Watch_UV1 Greater_Than, 5 {mAU}, Peak
(Peak)
0.00 Base SameAsMain
0.00 OutletValve Feed
0.00 Watch_UV1 Less_Than_Or_Valley, 4.75 {mAU}, Waste
(Waste)
0.00 Base SameAsMain
0.00 OutletValve Feed
0.00 Watch_UV1 Greater_Than, 5 {mAU}, Peak1
(Peak1)
0.00 Base SameAsMain
0.00 OutletValve Feed
0.00 Watch_UV1 Less_Than_Or_Valley, 4.75 {mAU},
waste1
(Waste1)
0.00 Base SameAsMain
0.00 OutletValve Feed
0.00 Watch_UV1 Greater_Than, 5 {mAU}, Peak2

• p 553
 F Method examples
F.5 Collection of three absorbance peaks

(Peak2)
0.00 Base SameAsMain
0.00 OutletValve Feed
0.00 Watch_UV1 Less_Than_Or_Valley, 4.75
{mAU}, Waste2
0.00 End_block
0.00 End_block
0.00 End_block
0.00 End_block
0.00 End_block
0.00 End_block

This is what hap- The table below describes what happens in the above example:
pens
Stage Description
1 When the UV reaches 5 mAU or more, the outlet valve is switched
to the position for collecting the first peak.

2 When the UV reading goes down to 4.75 mAU, the outlet valve
switches to the next position to separate the waste fraction from the
collected peak fraction.

3 This process is repeated twice for the next two peaks so that when
the UV reading rises above 5 mAU, the position switches to collect
the peak fraction and the position switches again to collect the waste
fraction when the UV reading falls again.

03-0014-90 • p 554
 Method examples F

F.6 Messages

When to use a Messages are used to inform the operator of the progress of the run. Messages can
message also be used for interaction between the operator and the system when necessary.
A message can be for information in a screen only, or it can require a signature
before the user can control the system. The messages are all added to the logbook
text. This appendix describes how to add a message to a method. The appendix
also gives two examples of how a message can be used.

How to add a The table below describes how to add a Message instruction to the method.
Message instruc-
tion
Step Action
1 • Select Other in the Instructions field of the Instruction box.
• Select Message in the instructions list.

2 Type a message in the Message text box in the Parameters field.

3 Select one of the display options on the Mode menu:

• Screen, i.e. only a text message is displayed.
• Noscreen, i.e. the message will not be displayed but only inserted
into the logbook.
• Authorize, i.e. the message will require a signature from the user
before the user can interact with the system again.

4 • Select a sound on the Sound menu if desired.

• Click the Insert button.

Note: If the Message instruction is inserted in a conditional block it will only be

displayed if the conditions of the block (for example a Watch) is fulfilled.
All messages are erased when the system reaches the End status. This also includes
Authorize messages.

Protecting a meth- A message can be set up in the beginning of a method to protect the method run
od run with a from unauthorized interference. Once the message is issued, the system is locked
message
from interaction by any user unless the user provides an authorization signature.
The only command that is available without authorization is Pause.
The illustration below shows the text instruction for the message described above:

• p 555
 F Method examples
F.6 Messages

Pausing a method A message can be set up to pause the method until a sample has been injected
run for a manual manually. If a message requiring an authorization is followed by a Pause instruction
sample injection
the system will be paused until the message is acknowledged and signed. No other
interaction with the system is available to the user. The operator will see a screen
with a reminder to inject the sample before the method run proceeds.
The illustration below shows the selected message instruction in the Instruction box
and the parameters for the message described above:

The illustration below shows the text instruction for the message described above:

Note: The message instruction must be followed immediately by the Pause

instruction as shown above.

03-0014-90 • p 556
 Method examples F

F.7 Quality control procedure

Introduction When a series of runs is performed, irregularities in samples or in system or column

performance can produce errors that will make the results inaccurate.
A quality control procedure can be added to a method to be used for a test run
during the series of runs. The control procedure can ensure that the results remain
within acceptable limits. If the result from the test run is unacceptable, the system
can be paused so that the error is not repeated in subsequent runs.

How to create the The easiest way to create the quality control procedure is to edit an existing
quality control procedure that includes a peak integration. The table below describes how to do
procedure
this.

Step Action
1 • Choose Procedures:Edit:Open in the Evaluation module.

Result: The Open Procedure dialog box opens.

2 • Select the procedure (Global) Integrate_and_Print.

• Click the OK button.

Result: The Procedure Editor opens with the procedure displayed.

3 • Select the REPORT instruction in the procedure.

• Choose Other and QC_TEST in the Instruction field.

4 • Type appropriate values in the Parameter field. See "QC_TEST

Parameter descriptions" below.
• Click the Replace button.

Result: The REPORT instruction is replaced by the QC_TEST instruc-

tion.

5 • Choose File:Save As.

Result: The Save As dialog box opens.

• Type a name for the procedure (for example QC_test).
• Select the Global procedure check box if the procedure is to be
available to all users.

Note: If you select File:Save to save the procedure it will replace the
(Global) Integrate_and_Print procedure.

• p 557
 F Method examples
F.7 Quality control procedure

Illustration: The The illustration below shows the Procedure Editor with the QC_TEST instruction
Procedure Editor displayed:

QC_TEST para- The table below describes the parameters for the QC_TEST instruction.
meter descriptions
The example values are used in the illustration above.

Parameter Description
Peak table source The peak table indicated in the PEAK_INTEGRATE in-
struction (Example: A).

Left limit The retention value where the control instruction will
begin (Example: 0).

Right limit The retention value where the control instruction will
end (Example: 100).
Note: The control instruction will be applied to the
run up to the sequence in the method where the con-
trol instruction is inserted:
• The controlled part of the run will end at the Right
limit if this retention value is reached before the
control instruction is reached in the method.
• If not, the controlled part of the run will end when
the control instruction is reached in the method.

Peak selection on The criteria for peak identification (Example: RETEN-

TION).

Order number The sequential order number of the peak (Example:

1).

03-0014-90 • p 558
 Method examples F

Parameter Description
Peak table parameter The peak table parameter that will be tested by the
control instruction (Example: RETENTION).

Less than Values less than the parameter value will be out of
the acceptable range (Example 10).

Greater than Values greater than the parameter value will be out
of the acceptable range (Example 11).

QC Action The action the system will take when the controlled
value is out of the acceptable range (Example: PAUSE).

Message text Free text message that is displayed when the con-
trolled value is out of the acceptable range (Example:
Retention out of range!)

Note: All values must be included before the instruction can be inserted.

How to add the The table below describes how to add the quality control procedure to a method.
quality control
procedure to a
method Step Action
1 • Open the method in the Method Editor.
• Click the Run Setup icon.

Result: The Run Setup for the method opens.

2 • Select the Evaluation Procedures tab.

• Click the Import button.

Result: The Import dialog box opens.

3 • Select the quality control procedure you created and saved (Ex-
ample: QC_test) in the Select field.
• Click the Import button.

Result: The quality control procedure is added to the available

evaluation procedures.
• Click the Close button.

4 • Click the check box to de-select the quality control procedure.

Note: If the quality control procedure is selected it will initiate a

new manual run at the end of the method run.

• p 559
 F Method examples
F.7 Quality control procedure

Step Action
5 • Click the Text Instructions icon.

• Select the last instruction in the method.

• Select Other:Evaluate in the Instructions field.
• Select the quality control procedure in the Procedure list.
• Click the Insert button.

6 • Choose File:Save

or
• Click the Save icon.

Result: When the method run is performed the quality control pro-
cedure will create a second chromatogram. If the controlled value
is outside the acceptable range, the system will be paused.

03-0014-90 • p 560
 Index

A
Alarms
Description, 461
Alarms and warnings
Description, 215
Effects on the system, 215
Analysis
External standard quantitation, 397
Recovery calculation, description, 406
Quantitation reliability factors, 408
How to create a quantitation table, 411
Molecular size determination, overview, 449
How to create a molecular size table, 452
How to calculate molecular size, 455
Analysis module
How to install, 391
Automated quantitation
Basic conditions, 439
How to prepare the quantitation table, 439
How to set up the sample runs, 441
Automatic update with Replace, 442
Automatic update with Average, 443
How to perform updates in scouting runs, 444
Automated update with Average for scouting runs, 447

B
Baseline
Calculation options, 329
The Calculate function, 329
Reuse existing, 329
How to edit manually, 349
How to adjust the baseline graphically, 351
Definition of a segment, 493
Parameters, 494
Batch run
How to perform, 384
BatchID
Logbook illustration, 205
Blank curve

•pi
 Index

Calculate baseline based on, 329

BufferPrep
Description, 143
Stock solutions, 143
How to create a method, 144
How to adjust the correction factors, 145
How to create a recipe, 537
Buffer concentration, 538
How to select the pH range, 539
How to define a new buffer substance, 539
How to define a new salt, 540
How to edit a recipe, 542
How to verify correction factors, 542
How to change correction factors, 543

C
Calibration curve
How to update, 425
Chromatogram Layout
Curve tab, 236
Default curve names, 236
How to choose curve name appearance, 236
The Curve Style and Color tab, description, 238
Chromatogram window
How to display header information, 229
Shortcut menu, 231
How to optimize the workspace, 231
How to display a vertical marker, 232
How to display the Logbook overlay, 232
Chromatograms
Description, 227
Temporary chromatogram, 227
How to make layout changes, general, 235
How to change and fix the Y-axis, 240
How to add a second Y-axis, 240
How to change and fix the X-axis, 241
How to save a layout, 242
How to apply a layout, 242
How to cut a curve and store as new, 245
How to change the size of fraction marks, 246

03-0014-90 • p ii
 Index

How to change the size of injection marks, 246

How to change the size of logbook marks, 246
How to print active chromatograms, 247
How to add annotations, 278
How to edit annotation text, 278
How to rename, 286
How to open several to compare, 302
The command File:Open to compare, 302
How to open several with the File:Open command, 303
How to display several simultaneously, 304
Commands to import curves from result files, 305
How to import curves with File:Open to compare, 306
How to import curves with File:Open, 309
How to copy curves into a new, 310
How to set a reference point, 364
Classic algorithm
Definition, 340
Parameters, 340
How to set, 340
Shortest baseline segment, 341
Slope limits, 342
Noise window, 344
Missing peaks, 345
When to change the Max baseline level, 346
How to set Max baseline level, 346
Definition, 493
How to measure baseline segments, 495
How to measure noise level, 496
How to measure the slope limit, 496
Columns
Column prompt in manual instructions, 212
How to add a new, 529
Normal column parameters, 530
How to edit parameters, 533
How to delete, 533
How to export, 533
How to import, 534
Concentration levels
Levels in quantitation, definition, 413
Conditional call
Description, 100

• p iii
 Index

Correlation
Explanation, 525
Curve fit models
Linear, 521
Linear through the origin, 522
Quadratic, 523
Quadratic through origin, 523
Point to point, 524
Curves
How to copy into the Temporary chromatogram, 227
Run curves default appearance, 231
How to choose the Y-axis scale, 231
Default curve names, 236
Peak labels, 238
Fraction text alignment options, 238
Logbook text alignment options, 238
How to change the color and style, 238
How to filter logbook information, 238
How to set a hatched background, 239
How to change and fix the Y-axis, 240
How to add a second Y-axis, 240
How to change and fix the X-axis, 241
How to save a layout, 242
How to apply a layout, 242
How to use the zoom function, 244
How to cut a curve and store as new, 245
How to reduce noise, 274
How to remove ghost peaks, 274
How to import a blank run curve, 276
How to subtract a blank curve, 276
How to add, 277
How to rename, 286
How to compare peaks in different curves, 288
Multifile Peak Compare Wizard, 288
Manual peak identification, 294
Commands to import curves into a chromatogram, 305
How to use the Open to compare command, 306
How to import using File:Open, 309
How to copy curves into one chromatogram, 310
How to align with Normalise, 312
How to move using the Shift function, 315
How to stretch or shrink using Multiply, 315

03-0014-90 • p iv
 Index

How to produce a mirror image, 316

How to shift a mirror image, 316
How to import, 319
Export options, 321
How to export, 321
How to export in AIA format, 323
How to delete unwanted curves, 326
How to divide, 366
How to reduce noise, 367
How to remove ghost peaks, 367
How to differentiate, 370
Simulated peak fractionation, 372
How to create, 373
Draw a straight curve between selected points, 376
How to create a fraction histogram, 377
Monitor signals stored as curves, 463
Curve settings, 463
Calibration curve models, 521
Molecular size curve models, 521
Curves pane in System Control
Description, 199
How to select curves to be monitored, 199
How to display a vertical marker, 199
How to set a reference point, 200
How to change curve colors and styles, 200
How to change scale of the Y-axis, 200
How to change scale of the X-axis, 201
How to zoom in regions of the pane, 202
Reduce scale of zoom, 202
How to select curve pressure units, 202
How to select text alignment, 202
How to display complete Logbook information, 203

D
Delete files and folders, 77
Delete Method blocks
How to use the Delete Block dialog box, 104
How to use the Block:Delete Block command, 105
How to delete unused blocks, 105
Documentation

•pv
 Index

How to view, 270

Documentation tabs, description, 270
Result information, 272
How to export, 324

E
Electronic signature
How to sign a result, 325
Evaluation
Chromatogram window views, 229
How to display chromatogram header information, 229
How to display peak table information, 230
Chromatogram window shortcut menu, 231
How to optimize the chromatogram workspace, 231
How to display a vertical marker, 232
How to set a reference point, 232
How to make chromatogram layout changes, general, 235
How to exit the module, 326
Evaluation logs
How to export, 324
Evaluation procedures
How to delete, 137
How to rename, 138
How to edit, 138
Explained variance
Definition, 525
External standard quantitation
How to perform, 397

F
File Navigator
How to open result files, 69
How to open, 222
How to locate files from the Files list, 222
How to use Find to locate a file, 223
How to open a recent run, 224
How to change preference settings for Recent Runs, 225
How to close, 225

03-0014-90 • p vi
 Index

Files and folders

How to copy, 75
How to move, 75
Copy to external, 75
How to copy from external, 76
Flow Scheme pane
Description, 204
Stretch to fit screen, 204
How to view manual instructions, 204
Folders
How to create a user-specific, 68
FPLCdirector
How to import data, 319
Frac-950
Defining the number of available tubes, 149
How to select the last tube, 150
Fraction Histogram
How to create a curve, 377
Fractions
How to set up the Frac unit, 149
How to view the contents of a fraction, 279
How to pool fractions, 279
How to create a pooled fraction curve, 280
Show only the pooled fraction curve, 281
How to calculate protein amounts and concentrations in pooled fractions, 282
How to determine the volume of a pooled fraction with a specific concentration, 282
How to use the Pool Fraction print list, 282

G
Generate Report Wizard
How to generate a report from the UNICORN Manager, 470
Gradient
Effects of Change and Replace on gradient length, 116
Gradients
Instruction parameters, 164
Step gradient instruction, 164
Gradient breakpoints, 164
Text instructions, 165
Define length as a variable, 165

• p vii
 Index

I
Instant Run
How to start, 191
Internal standard quantitation
Suitable components, 400
How to perform, 400
Reliability, 403

L
Linear flow rates
Description, 163
Log on and log off routines
How to start the program, 46
How to log on, 46
Log off alternatives, 47
Log off and set a password for a running process, 47
Unlock the system, 48
Quit UNICORN after log off, 49
Logbook
How to display an overlay in the Curves pane in System Control, 203
How to display an overlay in the chromatogram window, 232
How to filter the information, 238
Logbook pane
Description, 205
Autoscroll function, 205
How to filter the contents, 205
Search function, 206

M
Maintenance
How to view maintenance information, 465
How to set up a maintenance warning, 466
How to view warning parameters, 466
How to reset warning counters, 467
How to use the Generate Report Wizard from the UNICORN Manager, 470
How to use the Generate Report Wizard from the System Control, 473
Manual direct commands

03-0014-90 • p viii
 Index

Buttons in System Control, 208

Manual instructions in System Control
During a method run, 212
Column prompt, 212
Functions of buttons, 213
How to save results manually, 214
Measurements
How to make direct, 363
Messages
Usage, 160
How to issue, 160
How to add a message instruction to a method, 555
Method blocks
Description, 98
Blocks in the Text pane, 98
How to show or hide instructions, 98
Blocks in the Block pane, 99
Blocks in the Gradient pane, 99
Calls, 100
Unconditional calls, 100
Conditional calls, 100
Use the Instruction box to add, 101
Use the New Block dialog box to add, 102
Fields of the New Block dialog box, description, 102
Use the Delete Block dialog box, 104
How to use the Delete Block command, 105
How to delete unused blocks, 105
How to rename blocks, 106
How to find text strings, 107
How to copy a block, 107
How to move a block, 108
How to import, general information, 109
How to import, 109
Base instructions, descriptions, 155
Block length, 158
Method Editor
Modes, 28
Text instructions display panes, 29
The Block pane, 29
The Flow Scheme pane, 30
The Gradient pane, 30

• p ix
 Index

The Text pane, 30

The Instruction box, 31
Icon descriptions, 94
Log Format, 159
Method files
How to open in the UNICORN Manager, 69
How to connect a method to a system, 77
Method instructions
Instruction markings, 112
How to add an instruction, 113
Pause, Hold and Hold_until instructions, 113
How to delete instructions, 114
Undo delete, 114
How to change, 115
Difference in function between Change and Replace command, 115
Move an instruction within the same breakpoint, 117
Move an instruction to another breakpoint, 117
Instructions at the same breakpoints, 157
Method runs
Start from the UNICORN Manager, 190
Start from System Control, 190
How to define methods as menu commands, 190
How to start an Instant Run, 191
How to use the Start Protocol, 191
How to start a method run when the system is busy, 191
Run Data pane, description, 196
Curves pane in System Control module, 199
Flow Scheme pane, description, 204
Logbook pane, description, 205
Scouting runs, 216
How to perform a MethodQueue run, 217
If network communication fails, 219
Method templates
How to create a method, 85
Template information, 86
Save a new method, 86
How to create a template from a method, 171
How to delete a template, 171
Method Wizard
How to create a method, 81
How to save a new method, 83

03-0014-90 •px
 Index

MethodQueue
How to create a new, 183
How to use several systems in a queue, 184
Relative timing of steps, 185
Unattended execution, 185
Temporary hold when system is busy, 186
Folder handling, 187
File handling, 187
How to edit a MethodQueue, 187
How to perform a MethodQueue run, 217
Unattended operation, 217
Start when the system is busy, 217
How to display and edit pending and running MethodQueues, 218
Methods
How to create using a wizard, 81
How to sign, 91
Different method editing operations, 96
Method variables, general description, 118
How to select a column, 156
Hold instruction, description, 162
Pause instruction, description, 162
Hold_until instruction, description, 162
Linear flow rates, 163
Gradient instruction parameters, 164
How to print, 172
How to export, 174
How to export, 324
Monitor signal limits, 460
Molecular size calculation
Overview, 450
Preparations before curve creation, 451
How to create a size table, 452
How to open an existing table, 454
How to rename a molecular size table, 454
How to delete a molecular size table, 455
How to calculate the size, 455
Error signs, 457
Procedure instruction, 457
Molecular size curve
Overview, 449
Description, 453

• p xi
 Index

Morphological algorithm
Description, 336
How to set, 336
Structure width, 337
Incorrect structure width, 338
Noise window, 338
Minimum distance between points, 339
Definition, 493
Multifile Peak Compare Wizard
How to select the operation, 288
How to select data to compare, 290
How to select the peaks, 292
Manual peak identification, 294
How to select the Peak Data, 295
The Data View dialog box, 296
How to use the 2D data view, 297
How to use the 3D data view, 299
How to save the settings, 301
How to open saved settings, 301

P
Peak integration
How to perform, 330
Differences between to filter peaks and to reject peaks, 333
How to display peak labels, 334
How to select part of a curve for peak integration, 358
Peak skim
Compared to drop-lines, 360
How to select a ratio, 360
Peak table
How to display information, 230
How to rename, 286
How to export, 323
How to select contents, 364
Peaks
How to filter from view, 333
Labels, 334
How to display peak labels, 334
How to open the peak table, 351
How to delete a peak, 353

03-0014-90 • p xii
 Index

How to add a fill color and pattern, 354

Drop-lines, description, 355
How to split a peak, 355
How to join peaks, 356
How to add peak names, 356
How to exclude before integration, 359
Include negative peaks in integration, 359
How to select a skim ratio, 360
Edit integration for part of a curve, 361
Peak purity, 366
Peak identification through the absorbance ratio, 366
Peak parameters, 497
How to change the peak resolution algorithm, 500
How to change the Assymetry Ratio value, 502
Pool Fraction print list
How to use, 282
PrimeView
How to import data, 320
Problem reports
How to use the Generate Report Wizard from the UNICORN Manager, 470
How to use the Generate Report Wizard from the System Control, 473
Procedure instructions
Curve operations, 504
Integration, 507
File operations, 508
Export functions, 509
Chromatogram functions, 515
Miscellaneous, 516
Test instructions, 518
Procedures
How to record, 379
Global procedures, 380
How to build a procedure with instructions, 380
How to edit, 382
How to add instructions, 383
How to run a single procedure, 384
How to batch run, 384
How to rename, 387
How to delete, 387
Protein activity
Match to UV curve, 285

• p xiii
 Index

Protein amounts
Calculation formula, 282
Protein concentrations
Calculation formula, 282

Q
Quality control
How to create a control procedure, 557
How to add a control procedure to a method, 559
Quantitation
General description, 394
Process steps, 394
Procedure instructions, 396
How to use an external standard, 397
External standard reliability, 399
Internal standard, description, 400
How to use an internal standard, 400
Standard addition, description, 404
How to use standard addition, 404
Standard addition reliability, 405
Recovery calculation, description, 406
How to use recovery calculation, 406
Recovery calculation, reliability, 407
General reliability factors, 408
Preparations before, 410
Standard concentration levels, 410
Reject peaks, 410
How to create a quantitation table, 411
How to select table components, 414
Peak identification, 415
Relative retention, 416
How to adjust peak identification settings, 416
Peak identification criteria, 417
How to create a calibration curve, 418
How to enter standard data, 418
How to create a calibration curve, 418
How to enter standard data, 418
How to select an Internal Standard, 420
Statistics, 421
How to open a table for editing, 422

03-0014-90 • p xiv
 Index

How to update a calibration curve, 423

How to rename the table, 427
How to delete a quantitation table, 427
How to prepare for, 429
How to calculate the amount and concentration, 430
How to view the results, 431
Calculation error signs, 431
Standard addition stages, 432
How to prepare for standard addition, 432
How to select the standard addition component, 433
How to prepare for recovery calculation, 435
How to calculate the recovery, 435
Automated sample runs, 441
Automatic update with Replace, 442
Automatic update with Average, 443
Automated in scouting runs, 444
Automated update with Average for scouting runs, 447
Quick View
How to preview result files, 70

R
Recovery calculation
How to perform, 406
Reliability, 407
Recovery factor calculation
How to prepare for recovery calculation, 435
How to calculate the recovery, 435
Error signs, 436
Reference curves
In Run Setup, 140
Rename files and folders, 77
Reports
How to create a blank customized report, 250
Edit mode toolbar buttons, 251
How to add or delete pages, 252
How to change the page setup, 252
How to add objects to a report, 253
How to add free text, 254
How to add picture objects, 255
How to include chromatograms, 256

• p xv
 Index

How to include a peak table, 256

How to include a pool table, 256
How to include Method objects, 257
How to add documentation, 258
How to add the Evaluation log, 258
How to include Quantitate and Mol.Size data, 259
How to include Frac-950 data, 259
Toolbar icons in Report Edit Mode, 260
How to print, 261
How to save the report in PDF format, 262
How to save the report format, 263
How to create a Standard report, 264
How to print a standard report, 265
How to edit a standard report, 267
How to edit a customized report, 268
Result file name
Name options, 147
Serial numbers, 148
Unique identifier, 148
Result files
How to open in the UNICORN Manager, 69
How to open in the File Navigator, 69
Automated printing of, 136
Specify folder for storing, 148
How to open in UNICORN Manager, 221
How to open in UNICORN Manager, 221
Electronic signature, 325
How to save, 326
Run Data pane
Description, 196
How to change the appearance, 196
How to change text color or background, 197
How to set pressure units, 197
How to view and edit manual instructions, 197
Run Setup
Description, 28
Run Setup from the Method Wizard, 82
Tabs, description, 123
Variable tab view options, 125
How to change variable values, 125
Blue variable values, 125
How to delete variables, 126

03-0014-90 • p xvi
 Index

How to rename variables, 126

How to change a variable into a detail variable, 126
How to change a detail variable into a regular variable, 127
Scouting tab, 128
Questions tab, description, 129
Question status, alternatives, 129
Mandatory questions, 129
Authorized questions, 129
Chromatogram questions, 129
Questions, answer types, 129
How to insert a question, 130
How to edit questions, 131
Gradient tab, description, 133
Gradient zoom function, 133
Gradient marker line, 134
Gradient, change X-axis base, 134
Gradient hatch marks, 134
Notes, description, 135
How to write method notes, 135
How to search for text in the method notes, 135
Evaluation Procedures tab, 136
How to import evaluation procedures in the method, 137
How to edit method procedures, 138
Reference curves, 140
How to add reference curves, 140
Columns tab, 142
BufferPrep, description, 143
How to create a BufferPrep method, 144
BufferPrep correction factors, 145
Method Information tab, description, 146
Result Name tab, description, 147
Result file serial numbers, 148
Result file unique identifier, 148
Batch ID, 148
Start Protocol contents, 151
How to export values, 153
Scouting tab buttons, 176

S
Scouting
Specify folder for storing results, 148

• p xvii
 Index

Start Protocol settings, 152

Usage, 176
Changing variables, 176
Change variable values, 176
How to set up a Scouting Scheme, 177
How to edit a Scouting Scheme, 177
How to rename variables, 179
How to delete a variable, 179
How to change a variable into a detail variable, 179
How to copy variable content, 180
How to define columns, 181
How to perform a Scouting run, 216
Result files, 216
Scouting runs
Change of variables during a run, 216
Searches
General functions, 37
Security
Backup, 78
Set_Mark
Usage, 160
How to issue, 161
Slope values
Usage, 369
How to measure, 370
SMART Manager
How to import data, 319
Smoothing algorithms
Moving average, 490
Autoregressive, 490
Median, 491
Savitzky-Golay, 492
Snapshots
How to view, 41
How to add a text instruction, 90
Standard addition quantitation
How to perform, 404
Reliability, 405
How to identify sample peaks, 433
How to select the component, 433
Start Protocol

03-0014-90 • p xviii
 Index

How to use, 191

Statistics
Correlation, 525
Explained variance, 525
Stock solutions
Description, 143
Strategy
How to display the strategy instructions, 90
System Control module
Description, 32
Overview, 194
How to select the displayed panes, 194
How to customize the panes, 195
Toolbar buttons, 208
Manual instructions during a method run, 212
Manual instructions, 212
How to save manual results, 214
Alarms and warnings, 215
System Control status bar
Description, 210
Watch status, 211
System Control Toolbar
Manual command buttons, availability, 208
Manual command buttons, functions, 208
Windows buttons, 209
System Access buttons, 210
System data
How to backup, 63
How to restore backup data, 64
System operation
Automated workstation lock or logoff, 48
Unlock the system, 48
Unlock system locked by other users, 48
How to connect to a system, 58
Connection modes, 59
How to leave control of the system, 60
How to disconnect a system, 60
System settings
System summary, 61
How to change default settings, 459
How to assign a new value, 460

• p xix
 Index

T
Temporary chromatogram
Description, 227
Text instructions
Message instruction, 160
Set_Mark instruction, 161
Hold instructions, 162
Pause instruction, 162
Hold_until instruction, 162
Linear flow rates, 163
How to form a step gradient instruction, 164
Gradient breakpoints, 164
Gradient instructions, 165
Watch instructions, 166
How to insert a Watch instruction, 166
Watch parameter options, 167
Text Instructions Editor
When to use, 88
How to edit instructions, 88
Save a new method, 89
How to select panes, 95
Toolbar icons
In the System Control module, 33
Troubleshooting
Logon problems, 477
Strategy file error, 478
Access problems, 479
Connection problems, 479
Method problems, 482
Incorrect time and date, 487
Evaluation procedure aborts, 487
ÄKTAdesign system problems, 488

U
Unconditional call
Description, 100
Unconditional method instructions
Base instruction, 155
UNICORN Manager

03-0014-90 • p xx
 Index

Limited access to, 27

V
Variables
General description, 118
Identification in text instructions, 118
How to change method variable values, 119
Breakpoints or gradient lengths, 119
How to define new method variables, 119
Variable names, 120
How to rename a method variable, 120
How to remove a method variable, 121

W
Warnings
Description, 461
Watch instructions
Description, 100
Standard Watch conditions, 166
How to insert an instruction, 166
Parameter options, 167
Air sensors, 167
Permanent settings, 168
Temporary settings, 168
Delta_Peak settings, 168
Delta_Base settings, 169
Watch Stable_baseline, 170
Wizard
How to create a method, 81

Y
Y-axis
How to choose the Y-axis scale, 231

Z
Zero baseline

• p xxi
 Index

Definition, 329
Zoom function
How to enlarge parts of a curve, 244

03-0014-90 • p xxii