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Lab Report BCM 3

The experiments aimed to determine the optimum parameters for the rate of an enzyme reaction by measuring the effects of enzyme concentration, substrate concentration, and pH. The activity of the enzyme amylase was tested under varying conditions of these three factors. Experimentally, the graphs showed hyperbolic, straight line, and bell shapes for the effects of enzyme concentration, substrate concentration, and pH respectively on the reaction rate. Proper experimental design and minimizing errors can help obtain accurate and reproducible results for enzymatic reactions.

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Siti Nurasnizan Chie
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71 views2 pages

Lab Report BCM 3

The experiments aimed to determine the optimum parameters for the rate of an enzyme reaction by measuring the effects of enzyme concentration, substrate concentration, and pH. The activity of the enzyme amylase was tested under varying conditions of these three factors. Experimentally, the graphs showed hyperbolic, straight line, and bell shapes for the effects of enzyme concentration, substrate concentration, and pH respectively on the reaction rate. Proper experimental design and minimizing errors can help obtain accurate and reproducible results for enzymatic reactions.

Uploaded by

Siti Nurasnizan Chie
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© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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The experiments were conducted to determine the optimum parameter on rate of reaction of

enzyme. The evaluation involved enzyme concentration, substrate concentration and effect of ph.
The rate of reaction of enzyme was measured in absorbance by spectrophotometer.
The activity of enzymes was measured by the effect of enzyme concentration. Each cuvette
was filled with different enzyme concentration which are five, 15, 45 drops of enzymes with a
blank. Experimentally, the graph obtained is in …. shape. Theoretically, the graph for the effect of
enzyme concentration on the rate of reaction is in hyperbolic shape. By increasing the enzyme
concentration, the maximum reaction rate greatly increases. Enzymes can greatly speed up
the rate of are action. However, enzymes become saturated when the substrate concentration is
high.
Next, the effect of substrate concentration on the rate of reaction of amylase enzyme. Each
cuvette was filled with different substrate concentration which are one, three, five, 10, 20, 40 and
60 drops of starch with a blank. Experimentally, the graph obtained is in ….. shape. Theoretically,
the graph for the effect of substrate concentration on the rate of reaction is in straight line.
Increasing Substrate concentration will increase the rate of reaction. This is because
more substrate molecules will be colliding with enzyme molecules, so more product will be
formed.
The last one is the effect of pH on the rate of reaction of amylase enzyme. Different pH
buffers were used which are three, five, seven, nine and 11 pH buffers respectively.
Experimentally, the graph obtained is in ……… shape. Theoretically, the graph for the effect of
pH on the rate of reaction is in bell shape. Extremely high or low pH values generally result in
complete loss of activity for most enzymes. Generally, pH is also a factor in the stability of
enzymes. In addition to temperature and pH there are other factors, such as ionic strength, which
can affect the enzymatic reaction. Each of these physical and chemical parameters must be
considered and optimized in order for an enzymatic reaction to be accurate and reproducible.
Errors can happen in even the best experiments, but attention to detail and good
experimental design can help to minimize both random and systematic errors. Systematic errors
arise from either imperfection in the equipment being used, or by improper technique in the
laboratory. An example of a systematic error is using a cuvette that was stained or scratched, so
less light pass would through your sample and all readings using that cuvette would be affected.
Similarly, it is vital to properly clean and dry cuvettes, fill them using a pipette, handle them only
using gloves, and if possible, store them in a cuvette rack. Random errors are most likely to occur
because of the limitations of the equipment that you are using. However, selecting the correct tools
for the correct job can help minimize random errors. For example, an adjustable pipette will be
much better at measuring out a few milliliters of a solution. If random errors are unavoidable due
to equipment limitations, then the best way to minimize them is to repeat the experiment as many
times as possible to average out the error.

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