50

Screening of Indian bean against anthracnose JBiopest, 5 (supplimentary): 50-52 (2012) 50

Screening of Indian bean (Lablab purpureus L.) varieties/ germplasms

against anthracnose (Colletotrichum gloeosporioides Penz. and Sacc.)
under field conditions.
A. J. Deshmukh, B. P. Mehta, A. N. Sabalpara and V. A. Patil
ABSTRACT
Investigation on anthracnose (Colletotrichum gloeosporioides Penz. and Sacc.) of Indian bean (Lablab
purpureus L) under south Gujarat conditions was carried out in the Department of Plant Pathology, N. M.
College of Agriculture, Navsari Agricultural University, Navsari during the year 2008-2009 to find out suitable
management strategies. A field experiment was conducted in Rabi 2008 to screen five varieties and thirty nine
germplasms of Indian bean against anthracnose. Out of these, three varieties Kapasi, JNP-4, Katargam and two
germplasms NWP8 and NWP21 showed resistant reaction against anthracnose while fifteen germplasms viz.,
NWP12, 19, 20, 22, 24, 25, 26, 27, 28, 29, 30, 32, 35, 37, 39 were found to be moderately resistant, whereas variety
NPS1 was found highly susceptible to anthracnose of Indian bean under south Gujarat conditions.
Key words: Anthracnose, germplasms, screening, varieties.
INTRODUCTION MATERIALSAND METHODS
Indian bean (Lablab purpureus L.) is an important pulse crop About five varieties (obtained from different parents) and
of Gujarat. There are two cultivated types of Indian bean viz., thirty nine genotypes (obtained from same parents) of Indian
typicus and lignosus (Shivashankar et al., 1971). Typicus is a bean obtained from same parents were grown at Pulse
garden type and is cultivated for its soft and edible pods. Research Station, NAU Navsari in rabi 2008 by road row side
Lignosus is known as field bean and mainly cultivated for dry statistical method as seeds are available in very less quantity
seed as pulse and is more popularly recognized as ‘Wal’, and observations on per cent disease index were recorded by
‘Wal–papdi’ or ‘Valor’ in Gujarat state. The green pods are selecting 5 plants in each treatment. The disease intensity
used for vegetable purpose whereas; ripe and dried seeds are was recorded by observing three trifoliate leaves, first of base,
consumed as split pulse. The seeds can sometimes be soaked second of middle and third of upper portion of selected plant
in water overnight and when germination initiates, they can by using 0-6 standard scale. Observations were recorded at
be sun-dried and stored for future use. The fodder has good the initiation of the disease and at 15 days interval in field
palatability and the cattles are nourished well. It can also be condition starting from germination to harvesting and were
used as nitrogen fixing pulse crop. The fresh/immature pods graded as mentioned below (Palarpawar and Ghurde, 1989)
contain 4.5 per cent proteins and 10 percent carbohydrates viz., 0-Nil, 1-0.1-10% , 2-10.1-20 % , 3-20.1-30% , 4-30.1-40 %,
(Kay, 1973).Occurrence of anthracnose in popular variety 5-40.1-50 %, 6- >50.1%. The data was given here at about
NPS1 of walpapdi or Indian bean was observed seriously in 45 days after sowing.
south Gujarat in rabi 2007 and isolated and identified as C. Formula for calculating per cent disease index is
gloeosporioides. Out of various management strategies used,
use of resistant varieties is an ideal, the simplest and the Σ of ratings of plants infected
PDI = x 100
cheapest method for avoiding plant disease rather than No. of leaves observed x Maximum disease score
control. Moreover, it does not disturb field eco-system and Reaction of the disease was calculated on the basis of grades
avoids hazards of environmental pollution by avoiding as follows (Datar and Mayee, 1981)
fungicides spray to overcome disease. The identification of
the source of resistance is a basic need in breeding for disease PDI (%) - Reaction viz., 0.0-10%- Resistant (R), 10.1-20%-
resistance and hence the present investigation was carried Moderately Resistant (MR), 20.1-40% Moderately Susceptible
out and the results are reported here. (MS), 40.1-60% - Susceptible (S), above 60- Highly susceptible
(HS).
© JBiopest. 279
 Deshmukh et al. 51
RESULTS AND DISCUSSION
Three varieties Kapasi, JNP-4, Katargam and two germplasms
Out of five varieties and thirty nine germplasms screened NWP8 and NWP1 showed resistant reaction against
under field conditions, minimum per cent disease index was anthracnose while fifteen germplasms viz., NWP12, 19, 20, 22,
recorded in variety Kapasi, therefore, JNP4 and Katargam 24, 25, 26, 27, 28, 29, 30, 32, 35, 37, 39 were found to be
while in case of germplasms NWP8 and NWP21 were regarded moderately resistant. Thus it is suggested to discourage the
as resistant while fifteen germplasms viz., NWP12, 19, 20, 22, susceptible variety and to grow resistant to moderately
24, 25, 26, 27, 28, 29, 30, 32, 35, 37, 39 were found moderately resistant variety in south Gujarat area after rigorous testing
resistant, variety NPS1 showed highly susceptible reaction to maximize the production and also in varietal improvement.
(65.56 %) and germplasms NWP6 and NWP13 showed
susceptible reaction and other varieties/ germplasms were ACKNOWLEDGEMENT
moderately susceptible (Table 1).The varietal screening done
The authors express their gratitude to the Director of Research,
here was the first report of resistant and moderately resistant
the Dean of P.G. Studies, Navsari Agric. University, Navsari -
varieties. This is very useful information. It is suggested to
Gujarat for providing necessary facilities during the present
discourage the susceptible variety and to grow resistant to
investigations. They are also thankful to ITCC. IARI, New
moderately resistant variety in this area after rigorous testing
Delhi for providing identification of the pathogens.
to maximize the production.

Table 1. Reaction of different Indian bean varieties to C. gloeosporioides infection under field condition

Sr. Variety/ Percent Disease Disease Sr. Variety/germp Percent Disease

No germplasm Index reaction No lasm Disease reaction
(PDI ) Index
(PDI)
1. NPS1 65.56 HS 23 NWP20 13.33 MR
2. Manchhi 22.22 MS 24 NWP21 10.00 R
3. JNP 4 5.56 R 25 NWP22 18.89 MR
4. Kapasi 2.22 R 26 NWP23 25.56 MS
5. Katargam 7.78 R 27 NWP24 14.44 MR
6. NWP1 33.33 MS 28 NWP25 16.67 MR
7. NWP3 33.33 MS 29 NWP26 18.89 MR
8. NWP4 25.56 MS 30 NWP27 18.89 MR
9 NWP5 30.00 MS 31 NWP28 18.89 MR
10 NWP6 43.33 S 32 NWP29 17.78 MR
11 NWP7 27.78 MS 33 NWP30 17.78 MR
12 NWP8 5.56 R 34 NWP32 11.11 MR
13 NWP10 23.33 MS 35 NWP33 23.33 MS
14 NWP11 21.11 MS 36 NWP34 31.11 MS
15 NWP12 12.22 MR 37 NWP35 17.78 MR
16 NWP13 50.00 S 38 NWP36 25.56 MS
17 NWP14 33.33 MS 39 NWP37 14.44 MR
18 NWP15 36.67 MS 40 NWP38 33.33 MS
19 NWP16 27.78 MS 41 NWP39 16.67 MR
20 NWP17 26.67 MS 42 NWP40 27.78 MS
21 NWP18 22.22 MS 43 NWP41 26.67 MS
22 NWP19 14.44 MR 44 NWP42 26.67 MS
Navsari Wal Papdi (NWP), Navsari Wal Papdi Selection (NPS), Resistant(R), Moderately Resistant (MR), Moderately
Susceptible (MS), Susceptible(S), Highly susceptible(HS).
 Screening of Indian bean against anthracnose 52
REFERENCES Viswanatha S. R. 1971. Day- Neutral varieties of Dolichos
Datar, V. V. and Mayee, C. D. 1981. Assessment of losses in lablab L. Mysore Journal of Agricultural Sciences, 5: 216-
tomato yield due to early blight. Indian Phytopathology, 218.
34 (2): 191-195.
Kay, D. E. 1973. Food Legumes: Crop and product digest No. A.J. Deshmukh1*, B.P. Mehta2 , A.N. Sabalpara3
3. Tropical Product Institute, London, 303 P. and V.A.Patil4
Palarpawar, M. Y. and Ghurde, V. R. 1989. Sources of resistance 1,2-Department of Plant Pathology, N.M. College of
in turmeric against leaf spots incited by Colletotrichum Agriculture,Navsari, India.
capsici and C. curcumae. Indian phytopathology, 42: 171- 3,4-Director of Research and Dean, P.G. Studies, Navsari
173. Agricultural University, Navsari, India. *E-mail:
Shivashankar, G., Srirangasayi, I., Kempanna, C. and [email protected]

Received: September 20, 2011 Revised: October 14, 2011 Accepted: January 18, 2012