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BioprocessProblem 3

This document contains three problems related to continuous stirred-tank fermenters (CSTFs). Problem 1 involves analyzing experimental data to determine rate equations for cell growth and product formation in a CSTF. Problem 2 involves determining optimal fermenter sizes, concentrations, and flow rates using a Monod kinetics model. Problem 3 involves calculating steady-state concentrations in a CSTF with a cell recycling system using Monod kinetics parameters.

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403 views1 page

BioprocessProblem 3

This document contains three problems related to continuous stirred-tank fermenters (CSTFs). Problem 1 involves analyzing experimental data to determine rate equations for cell growth and product formation in a CSTF. Problem 2 involves determining optimal fermenter sizes, concentrations, and flow rates using a Monod kinetics model. Problem 3 involves calculating steady-state concentrations in a CSTF with a cell recycling system using Monod kinetics parameters.

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Ashenafi
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© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Problem 4.

1. Aiba et al. (1968) reported the results of a chemostat study on the growth of a specific strain
of baker's yeast as shown in the following table. The inlet stream of the chemostat did not
contain any cells or products.

a. Find the rate equation for cell growth.


b. Find the rate equation for product (ethanol) formation.

2. Suppose you have an organism that obeys the Monod equation:

where max = 0.5 hr-1 and Ks = 1 g/L.


The organism is being cultivated in a steady-state CSTF, where F = 100 L/hr, CSi = 50 g/L,
and YXIS = 0.5.
a. What size vessel will give the maximum total rate of cell production?
b. What are the substrate and cell concentrations of the optimum fermenter in part (a)?
c. If the exiting flow from the fermenter in part (a) is fed to a second fermenter (CSTF),
what should be the size of the second fermenter to reduce the substrate concentration to
1 g/L?
d. If the exiting flow from the first fermenter in part (a) is fed to a second fermenter whose
size is the same as the first, what will be the cell and substrate concentrations leaving
the second fermenter?

3. A strain of yeast is being cultivated in a 30-L CSTF with a cell recycling system (cell
settler) as shown in the following figure. The cell settler was designed so that the cell
concentration of its outlet stream is 30 percent of that of its inlet stream, whereas the
substrate concentrations of the two streams are the same. The growth rate of the cells
can be represented by the Monod kinetics with the parameters: Ks =0.05 g/L, max =0.3
h-1 and YXIS = 0.025. Calculate the steady-state substrate and cell concentrations in the
fermenter. The inlet substrate concentration is 100 g/L and the flow rate is 20 L/hr. The
feed stream is sterile.

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