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    Isolation of Caffeine From Tea

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    mokilpo
    This document provides instructions for isolating caffeine from tea through extraction and purification. Key steps include: 1) Extracting tea with water and sulfuric acid, then filtering and extracting the filtrate with chloroform to isolate caffeine, which is more soluble in chloroform than other compounds. 2) Analyzing the purified sample using thin layer chromatography alongside reference samples of caffeine, theobromine, and theophylline to identify compounds. 3) Obtaining an infrared spectroscopy spectrum for compound identification and comparison to reference spectra. The goal is to isolate and identify caffeine from tea.

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    Isolation of Caffeine From Tea

    Uploaded by

    mokilpo
    383 views2 pages
    This document provides instructions for isolating caffeine from tea through extraction and purification. Key steps include: 1) Extracting tea with water and sulfuric acid, then filtering and extracting the filtrate with chloroform to isolate caffeine, which is more soluble in chloroform than other compounds. 2) Analyzing the purified sample using thin layer chromatography alongside reference samples of caffeine, theobromine, and theophylline to identify compounds. 3) Obtaining an infrared spectroscopy spectrum for compound identification and comparison to reference spectra. The goal is to isolate and identify caffeine from tea.

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    This document provides instructions for isolating caffeine from tea through extraction and purification. Key steps include: 1) Extracting tea with water and sulfuric acid, then filtering and extracting the filtrate with chloroform to isolate caffeine, which is more soluble in chloroform than other compounds. 2) Analyzing the purified sample using thin layer chromatography alongside reference samples of caffeine, theobromine, and theophylline to identify compounds. 3) Obtaining an infrared spectroscopy spectrum for compound identification and comparison to reference spectra. The goal is to isolate and identify caffeine from tea.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    383 views2 pages

    Isolation of Caffeine From Tea

    Uploaded by

    mokilpo
    This document provides instructions for isolating caffeine from tea through extraction and purification. Key steps include: 1) Extracting tea with water and sulfuric acid, then filtering and extracting the filtrate with chloroform to isolate caffeine, which is more soluble in chloroform than other compounds. 2) Analyzing the purified sample using thin layer chromatography alongside reference samples of caffeine, theobromine, and theophylline to identify compounds. 3) Obtaining an infrared spectroscopy spectrum for compound identification and comparison to reference spectra. The goal is to isolate and identify caffeine from tea.

    Copyright:

    © All Rights Reserved
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    of 2

    MSc practical

    ISOLATION OF CAFFEINE FROM TEA

    Introduction

    The principle xanthines used in medicine are caffeine and theophylline. This group of drugs is
    of value for their effects on the kidney, smooth muscle of the bronchi and the central nervous
    system. (Theophylline is widely used as a bronchodilator in asthma).
    Caffeine and its salts are most active in their stimulation of the central nervous system and are
    principally used for this purpose.

    Caffeine is administered in powder or tablet form and is frequently included in analgesic


    preparations with aspirin, paracetamol, or codeine. Caffeine is claimed to enhance the activity
    of analgesics and also ergotamine, and is often added to “tonics” for tiredness. It may be
    obtained from tea or coffee or prepared synthetically.

    The object of this exercise is to isolate caffeine from tea. Identification of the caffeine should
    be made by IR and TLC

    1) Method of isolation of methylxanthines

    1. Tea (~10g weighed to 4 decimal places) is extracted with water (200ml) plus 0.05N
    sulphuric acid (50ml) in a beaker using a Bunsen bring to boil then remove from heat.

    2. Leave to cool for a few minutes and filter using Buchner system.

    3. Add to the filtrate 20 ml of 10% sulphuric acid and stir.

    Sulphuric acid converts the tannins to their salts, thus rendering them insoluble in chloroform
    but they are soluble in water

    4. The filtrate should now be extracted by 3 successive washes (in a separating funnel) of
    chloroform (50ml each) .With each wash collect the organic layer (Bottom)

    caffeine is more soluble in chloroform than the tannins.

    5. Combine all the extracts.

    6. Dry by adding large spatulas of anhydrous sodium sulphate until water is removed. It is dry
    when you get a fine powder.

    7. Clean Buchner system and dry using acetone in the fume cupboard.

    8. Remove sodium sulphate by filtering with Buchner system.

    9. Pour the filtrate into a pre-weighed (to 4 decimal places) round-bottomed flask.
    10. Evaporate all the chloroform using a rotary evaporator (see demonstrators) you will be left
    with methylxanthine crystals.

    2) Thin Layer Chromatography

    After obtaining a yield for your methylxanthines re-dissolve your crystals in 2ml of
    chloroform. Examine 1ml of your solution, as well as reference solutions and mother liquor by
    TLC.

    Methylanthines absorb UV light and therefore the inclusion of a fluorescent agent in the
    stationary phase is particularly useful since visualisation in the UV at 254nm can then be used.

    Plates: Silica gel GF254


    Solvent: Chloroform: Acetone: N-Butanol: 30% Ammonia, (30:30:40:10)
    Reference solutions: Caffeine, Theobromine, Theophylline, and Mother Liquor

    3. Infra Red Spectroscopy

    Using the rest of your methylxanthine solution obtain an IR spectrum

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