TARGET GATE

THE HYDERABAD ACADEMY

Name: Sub : Pharmaceutical Analysis
ID : Topic: U.V.Visible
Test :1 Date :
1. Match the following

P) X-rays i) 1-100A
Q) Visible rays ii) 40000A-80000A
R) IR rays iii) 20000A-40000A
S) Vaccum UV rays iv) 25x103 0A-250x103 0A
T) UV rays v) <20000A

A) P-i B) P-iv C) P-v D) P-i

Q-iii Q-ii Q-iv Q-ii
R-iv R- i R-iii R-iii
S-v S-iii S-ii S-iv
T-ii T-v T-i T-v

2. Chemical reaction applied for photometric evaluation of vitamin-A

a) Bayers test b) Ferric chloride test
c) Carrprice test d) None of the above

3. Colour observed in carrprice reaction

a) Red b) Green
c) Yellow d) Blue

4. Reagent responsible for blue colour development in carrprice reaction.

a) AgNO3
b) Antimony trichloride
c) Saturated solution of antimony trichloride in CHCl3
d) None of the above

5. Shark liver oil gives positive test to the following

a) Carrprice reaction b) Reimer-tiemar reaction
c) Meerwein-pondroff reduction d) All the above

6. Thickness of the sample medium in UV-Visible spectroscopy is related

a) Beers law b) Lamberts law
c) Beers-Lamberts law d) All the above

Common data for questions 7, 8 and 9

One milli liter of 0.1%w/v Paracetamol solution was diluted to 100 ml. The
absorbance of diluted solution was 0.253 at (1 cm) cell (molecular weight of
paracetamol-151.17)

1
7. Molar absorbitivity is
a) 36.24x104 b) 40.24x104
c) 38.24x104 d) 32.24x104

8. What is concentration of unknown solution having 0.91 absorbance at 1cm

cell.
a) 3.196x10-4g/lit b) 3.596 x10-4g/lit
-4
c) 2.596x10 g/lit d) None of the above

9. Find the concentration of unknown solution having absorbance 0.82 in 2cm

cell.
a) 1.52x10-4g/lit b) 1.42x10-4g/lit
c) 1.82x10-4g/lit d) 1.62x10-4g/lit

10. Most stable excited electron in the following.

a) Single excited state
b) Double excited state
c) Triple excited state
d) None of the above

11. Broad absorption band in UV spectra is due to

a) Electronic transition.
b) Vibrational motion of molecules.
c) Rational motion of molecules.
d) All the above

12. Presence of hydrogen bonding causes

a) Hyperchromic shift b) Hypochromic shift
c) Blue shift d) Red shift

13. Increase in polarity of solvent generally shifts the n-π* band towards.
a) Shorter wavelength b) Longer wavelength
c) Hypochromic shift d) None of the above

14. Most commonly used solvent in UV-visible spectroscopy

a) Water b) Alcohols
c) CCl4 d) None of the above

15. Primary filter absorbs following radiation.

a) UV radiation b) IR radiation
c) Visible radiation d) X-rays

16. Secondary filter emits following radiation

a) IR radiation b) X-rays
c) Visible radiation d) UV radiation

2
 TARGET GATE
THE HYDERABAD ACADEMY
Name: Sub : Pharmaceutical Analysis
ID : Topic: IR
Test :2 Date :

1. Match of the following IR region with their respective wave number.

Region Wave number
a) Near IR i) 1500-600cm-1
b) Far IR ii) 667-50cm-1
c) Middle IR iii) 12500-4000cm-1
d) Finger print iv) 4000-667cm-1
v) 1500-100cm-1
A) a-iii B) a-i C) a-ii D) a-iv
b-ii b-iii b-iv b-i
c-iv c-ii c-i c-iii
d-i d-iv d-iii d-ii

2. The most important region for an organic chemist to detect and measure
molecular vibrations in an IR-spectra
a) 2.5μ to 15μ b) 14.3μ to 50μ
c) 0.7μ to 2.5μ d) 6 μ to 13μ

3. The IR absorption occurs when

a) Vibrational frequency equals to IR frequency
b) Processional frequency equals to IR frequency
c) Vibrational frequency equals to processional frequency
d) None of the above

4. Predominant changes in the vibrational energy depends on the following one

a) Masses of the atoms present in the mole.
b) Strength of the bonds.
c) Arrangement of the atoms with in the molecule.
d) All the above.

5. In which of the following the bond angle remain constant.

a) Symmetric stretching b) Asymmetric stretching
c) Twisting d) Both a and b

6. The vibrational frequency or wave number depends on

a) Bond length b) Reduced mass
c) Velocity of radiation d) All the above

3
 Common data for Questions 7, 8 & 9
When Hydrocortisone subjected to MPV reduction

7. What is the resultant product?

a) C3-10OH and C20-20OH b) C3-20OH and C20-10OH
c) C3-20OH and C20-20OH d) None of the above

8. The IR band present in starting material disappears in final product IR spectra is

a) 1800-1000cm-1 b) 2800-2000cm-1
-1
c) 3650-3600cm d) 1200-1000cm-1

9. The broad peak appears in IR spectra above region 3000 cm-1 is due to
H

C O C OH
a) b)

C C
c) C OH d) C OH

10. The O-H streching absorbs at high frequency than C-C streching is due to
a) Lower reduced mass of O-H than C-C
b) Lower redused mass of C-C than O-H
c) Decrease in bond strength of O-H than C-C
d) Both a and c

11. The theoretical fundamental bands for the linear molecules will be
a) 3n-5 b) 3n-6
c) 3n-4 d) 3n-7

12. The number of vibrational degree of freedom in benzene is

a) 36 b) 30
c) 32 d) 34

13. What is the right option in the following for IR spectra?

a) 1-chloro ethanol wave number is higher than methane.
b) 1-chloro ethanol wave number is lower than methane.
c) Methane wave number is equal to 1-chloro ethanol.
d) None of the above.

14. Fermi-resonance occurs when

a) Energy of overtone more than fundamental mode
b) Energy of fundamental mode than overtone
c) Energy of overtone coincides with the fundamental mode.
d) None of the above.

4
15. Match the following compounds with respect to their wave number.
P) Formaldehyde i) 1725cm-1
Q) Acetone ii) 1740cm-1
R) Chloro acetone iii) 1750cm-1
S) Di chloro acetone iv) 1715cm-1

a) P-iii b) P-iii c) P-ii d) P-iv

Q-ii Q-iv Q-iv Q-i
R-iv R-i R-i R-iii
S-i S-ii S-iii S-ii

16. Which of the following electronic effect influence the vibrational frequencies in
IR?
a) Inductive effect b) Mesometric effect
c) Field effect d) All the above

17. When the compound is para substituted, the wave number of absoption in IR
region is decided by
a) Inductive effect b) Mesometric effect
c) Field effect d) Both a and b

18. Which of the following is not present in FT-IR spectrometer?

a) Movable mirror b) Interferometer
c) Monochromator d) None of the above

19. Which of the following substance used to mix the sample in Mull technique for IR
analysis?
a) Nujol b) Nujol in combination
c) Both a and b d) None of the above

20. GROUP-I GROUP-II

P. O-H stretching in IR vibration 1. 3500-3300cm-1
Q. C=O vibration 2. 1280-1000 cm-1
R. N-H vibration 3. 3700-3350 cm-1
S. C-O vibration 4. 1950-1640 cm-1

a) P-3 b) P-4 c) P-2 d) P-2

Q-4 Q-1 Q-4 Q-3
R-1 R-2 R-1 R-1
S-2 S-3 S-3 S-4

21. Gas cell which is used for sampling of gases in IR is made up of

a. KBr or NaBr b. KCl or NaCl
c. Both a & b d. None of the above

5
22. Match the following substances with their respective transmission ranges in IR
radiation.

P. NaCl 1. 9.0-26.0μm
Q. KBr 2. 2.0-15.4 μm
R. CaF 3. 2.5-5.9 μm
S. LiF 4. 2.4-7.7 μm

a) P-3 b) P-4 c) P-2 d) P-2

Q-4 Q-1 Q-1 Q-3
R-1 R-2 R-4 R-1
S-2 S-3 S-3 S-4

23. Which of the following used in construction of traditional infrared spectro

photometers?
a. Monochromator b. Interferometer
c. Both a & b d. None of the above

24. Possible representations for IR spectra

a. % T vs Wave number b. Absorption vs Wave number
c. Both a & b d. None of the above

25. Which of the following effect is considered when the compound is meta
substituted?
a. Field effect b. Inductive effect
c. Resonance effect d. Hypochromic effect

6
 TARGET GATE
THE HYDERABAD ACADEMY
Name: Sub : Pharmaceutical Analysis
ID : Topic: Fluorimetry,FES, XRD, AAS
Test :3 Date :

1. UV, Visible spectroscopy is an example of

a) Absorption spectroscopy b) Emission spectroscopy
c) Molecular spectroscopy d) Both a and b

2. Technique in which emission of radiation are measured

a) UV spectroscopy b) Visible spectroscopy
c) Fluorimetry d) IR spectroscopy

3. GROUP-I GROUP-II
P) Absorption i) Transition from singlet
exited state to triplet state
Q) Fluoroscence ii) Transition of electron from triplet
state to singlet ground state
R) Phosphorescence iii) Transition of electrons from
singlet exited state to singlet
ground state
iv) Transition of electrons from
singlet ground state to singlet
exited state

a) P-4 b) P-4 c) P-2 d) P-2

Q-3 Q-1 Q-1 Q-3
R-2 R-2 R-4 R-1

4 GROUP-I ` GROUP-II
a) Stokes flouroscence i) λ of emitted radiation is shorter the
absorbed radiation
b) Anti-stokes flouroscence ii) λ of emitted radiation in equal to
the λ of absorbed radiation
c) Resonance flouroscence iii) λ of emitted radiation longer than
λ of absorbed radiation

A) a -2 B) a -3 C) a -2 D) a -1
b -3 b -1 b -1 b -2
c -1 c -2 c -3 c -3

5. Flouroscence intensity -----------on conjugation

a) Increases b) Decreases
c) No effect d) Both a and b

7
6. On substitution with electron donating group, fluorescence intensity
a) Increases b) Decreases
c) No effect d) Both a and b

7. Fluorescence intensity decreases

a) Due to increase in temperature b) Decrease in viscosity
c) Oxygen d) All the above

8. Quenching ---------- fluorescence intensity

a) Increases b) Decreases
c) No effect d) Both a and b

9. Match the following elements with respect to their emitted radiation.

Element Emitted radiation
P) Sodium i) Crimson brilliant white
Q) Potassium ii) Orange radiation
R) Calcium iii) Yellow radiation
S) Lithium iv) Blue radiation
v) Brick red colour

a ) P-iii b) P-ii c) P-i d) P-iv

Q-ii Q-iv Q-v Q-iii
R-v R-iii R-ii R-i
S-i S-v S-iv S-ii

10. Most commonly used fuel in a conventional flame photometer.

a) Propane b) Hydrogen
c) Liquified petroleum gas d) None of the above

11. The I A group elements mostly emits the radiation in

a) Visible region b) UV region
c) Vaccum region d) Both a and b

12. If the percentage flame intensity of a sample is more than 100%. How can you
estimate the concentration of substance.
a) By diluting sample b) By changing the filter
c) By using internal standard d) Both a and b

13. Standard addition method in flame photometry is

a) Removal of interfering elements from sample matrix is difficult.
b) To overcome physical and anionic interference.
c) Both a and b
d) To avoid cationic interference.

8
14. The most suitable technique for quantitative analysis of calcium present in a
a given compound.
a) Flame photometry b) AAS
c) UV-spectroscopy d) All the above

15. Spectral line interference is also known as

a) Molecular spectral interference b) Cation-cation interference
c) Ionization interference d) Vapourisation interference
e) Both a and b

16. Which of the following is most suitable for estimation of ZMC in Zinc insulin
Injection?
a) Flame emission b) AAS
c) Fluorimetry d) None of the above

17. The concentration of element in AAS is depends on

a) Radiation absorbed b) Radiation emitted
c) Excited atoms d) Ground state atoms
e) Both a and d

18. Match the following elements emitted by AAS with the wavelength
P) Lead i) 240.7
Q) Nickel ii) 248.3
R) Cobalt iii) 232
S) Iron iv) 217
v) 253
A) P-iii B) P-ii C) P-i D) P-iv
Q-ii Q-iv Q-v Q-iii
R-iv R-iii R-ii R-i
S-i S-v S-iv S-ii

19. Emission of radiation of longer wavelength immediately is called

a) Fluorescence b) Phosphorescence
c) Luminescence d) Both b and c

20. The electron transition in phosphorescence is from

a) Singlet ground state to singlet excited state.
b) Singlet excited state to singlet ground state.
c) Doublet excited state to singlet ground state.
d) None of the above

21. Purpose of the monochromator in atomic absorption spectroscopy

a. To isolate the resonance lines from all non absorbed lines
b. To isolate the non absorbed lines from the resonance lines
c. Both a & b
d. None of the above

9
22. Ionisation of ground state atoms in flame photometry causes
a. Reduce the intensity of emission lines
b. Increases the intensity of emission lines
c. Moderate effect on intensity of emission lines
d. No effect

23. Match the following.

Element Atomic Excitation Wavelength
P. Sodium 1. 422.7nm
Q. Calcium 2. 589nm
R. Zinc 3. 213.9nm
4. 322nm

A) P-2 B) P-1 C) P-3 D) P-4

Q-1 Q-2 Q-2 Q-2
R-3 R-3 R-4 R-1

10
 TARGET GATE
THE HYDERABAD ACADEMY
Name: Sub : Pharmaceutical Analysis
ID : Topic: Fluorimetry, FES, XRD
Test :4 Date :
1. Sources used in flourimeter
a) Mercury vapour lamp b) Xenon arc lamp
c) Tungstan filament lamp d) All of the above

2. Arrange the following in decreasing energy order

a) ΔE (Absorption) > ΔE (Flouroscence) > ΔE (Phosphorescence)
b) ΔE (Flouroscence) > ΔE (Absorption) > ΔE (Phosphorescence)
c) ΔE (Phosphorescence) > ΔE (Flouroscence)> ΔE (Absorption)
d) None of the above

3. Arrange the following in the increasing order of a corresponding to the ΔE value

a) λ (Absorption) > λ (Flouroscence) > λ (Phosphorescence)
b) λ (Flouroscence) > λ (Absorption) > λ (Phosphorescence)
c) λ (Phosphorescence) > λ (Flouroscence)> λ (Absorption)
d) None of the above

4. In dilute atomic vapour--------- flouroscence occur

a) Resonance b) Stoke’s
b) Anti stoke’s d) None of the above

5. Which of the following fluorescence occurs in more complex organic compounds

a) Resonance b) Stoke’s
b) Anti stoke’s d) Both a and b

6. Concentration up to-------- can be measured by flourimetry

a) 10-2 M b) 10-5M
c) 10-3M d) 10-7M

7. The unpaired electron of same spin is called

a) Singlet excited state b) Triplet state
c) Both a and b d) Singlet ground state

8. The favorable conditions for phosphorescence is

a) Low temperature b) High temperature
c) Absence of oxygen d) Both a and c
e) Both b and c

9. The effect of SO3H and NH4 on fluorescence intensity.

a) Increases b) Decreases
c) No effect d) None of the above

11
10. Rigid structure of conjugated compounds produces.
a) More fluorescence intensity b)Less fluorescence intensity
c) Non-fluorescence d) None of the above

11. Aniline in acidic conditions gives

a) UV region b) Visible region
c) Non-fluorescent d) None of the above

12. The fluorescent intensity of riboflavin is reduced by caffeine is due to

a) Chemical quenching b) Static quenching
c) Self quenching d) Collisional quenching

13. Hydrocortisone can be estimated by flouremetry when it reacts with

a) Formaldehyde b) Fluorescein
c) Cyanogen bromide d) 75%w/v H2SO4 in ethanol

14. Which of the following is not fluorescent indicator?

a) Eosin b) Quinine sulphate
c) Acridine d) 2-naptha quinine
e) None of the above

15. X-ray diffraction is based on

a) Diffraction b) Reflection
c) Scattering d) Refraction
e) All the above

16. The most useful X-ray region in medical practice is

a) 0.1 to 1000A b) 0.7 to 2.00A
c) 10 to 1000A c) 0.5 to 2.00A
0
d) 0.9 to 2 A

17. Generation of ‘K-lines’ in X-ray’s is due to

a) Transfer of electron from ‘K’ orbital to ‘C’ orbital.
b) Transfer of electron from ‘L’ orbital to ‘K’ orbital
c) Transfer of electron from ‘K’ orbital to ‘M’ orbital
d) Nome of the above

18. Which of the following property of metal can be analysed by X-ray defraction?
a) Annealing b) Metal fatigueness
c) Conductance d) Both a and b
e) None of the above

19. The amount of amorphous powder in a crystalline compound in X-RD spectra can
be represented by
a) Sharp peak b) Broad peak
c) White background d) All the above

12
 TARGET GATE
THE HYDERABAD ACADEMY
Name: Sub : Pharmaceutical Analysis
ID : Topic: Electro chemical methods
Test : 5 Date :
1. Example for reference electrode except
a) Antimony electrode b) Silver-silver electrode
c) Calomel electrode d) None of the above

2. Nature of the indicator electrode used in redox titrations

a) Acidic b) Inert
c) Oxidative d) Reductive

3. Principle involved acid base potentiometric titrations

a) EMF of cell α 1/pH b) EMF of cell α /pH
c) EMF of cell α / 2pH d) EMF of cell α / 1/2pH

4. External indicator used in diazotization titration is

a) Starch solution b) Phenolphthalein
c) Iodine solution d) None of the above

5. Oxidation states of ‘Mn’ in KMnO4 redoxtitration

a) 7, 5 b) 7, 3
c) 7, 2 d) None of the above

6. Principle involved in cericammonium sulphate redox titrations

a) Fe reduces Ce b) Ce reduces Fe
c) Ce oxidizes Fe d) Fe oxidizes Ce

7. The indicator used in estimation of hardness of water by potentiometry

a) PH b) PM
c) Both a and b d) None of the above

8. Number of rings observed in the tetravalent ion EDTA complex

a) 4 b) 5
c) 6 d) 3

9. Stability of the complex formed in complexometric titration is reduced by

a) Addition of acid b) Addition of base
c) Addition of ethanol d) Both a & b

10. Use of mercury as a microelectrode in polarography is due to.

a) Reproducible surface area with any given capillary
b) Mercury forms amalgams with many metals
c) Mercury may be early oxidized d) Both a, b and c
e) None of the above

13
11. Reason for generation of migration current in polarogram is
a) Migration and diffusion of charged particles
b) Migration of charged particles
c) Diffusion of charged particle d) None of the above

12. Rapid analysis of mixture of organic and inorganic samples with out separation
even at low concentration .this technique is
a) Potentiometry b) Conductometry
c) Ampherometry d) Polarography

Common data for question 13&14

“Royal pharma Pvt.ltd manufacturer adrenaline injections of 2μg/ml,
10μg/ml, 20μg/ml”

13. Adrenaline pocess following activity

a) Vasoconstrictor b) Vasodilation
c) Selective β-stimulation d) Inotropic

14. Following method is suitable for analysis of adrenaline injection is

a) Polarography b) Potentiometry
c) Conductometry d) Ampherometry

15. Following is analysed by polarography

a) Amlodopine b) Chloramphenicol
c) Vanillin d) All the above

16. Organometallic compounds analysed by the following technique

a) Polarography b) Conductometry
c) Complexometric titration d) Potentiometry

17. Unit of measurement for conductance

a) Ohm-1 b) mho
c) Siemens d) All of the above

18. Conductivity of water for

a) < 5μg/ml b) < 6μg/ml
c) < 2μg/ml d) None of the above

19. Chemical used in the determination of cell constant for electrode

a) NaCl b) KCl
c) MgCl2 d) Both a and b

20. Assay of Sulfanilamide by titrimetry needs the following medium.

a. Acidic b. Basic
c. Neutral d. Non polar

14
 TARGET GATE
THE HYDERABAD ACADEMY
Name: Sub : Pharmaceutical Analysis
ID : Topic: Mass spectroscopy
Test : 6 Date :
1. Positive charged particle in the plane of magnetic field shows.
a) Anti-clockwise rotation. b) Clockwise rotation
c) Three dimensional d) None of the above

2. Reason for the presence of circular path in dempster mass spectrometer is

a) Positive ions rotates in a vaccum.
b) Positive ions rotates clockwise in the plane of magnetic field.
c) Both a and b d) None of the above

3. Application of vaccum in mass analyzer is due to

a) Prevent collision between positive and negative ions.
b) Prevent collision between positive ions.
c) Both a and b d) None of the above
4. The region present in time of flight mass analyzer.
a) Magnetic field region b) Electric region
c) Vaccum region d) None of the above

5. Parent ion abundance is more in the following.

a) Alkanes b) Fatty acids
c) Aromatic compounds d) None of the above

Common data for questions 6, 7, and 8

The broad peak was observed when para aminoanisol is analysed for mass
spectum with the use of double beam mass spectrometer.
6. The broad peak is due to
a) Molecular ion b) MR ion
c) Metastable ion d) Fragment ion

7. The broad peaks are generated due to presence of following region.

a) First field free region. b) Second field free region.
c) Third field free region. d) Vaccum region.

8. Position of broad peak in mass spectra is (m/z)

a) 91 b) 90.8
c) 94.8 d) None of the above

9. Mass chromatography lafferty rearranged ions are observed in the following.

a) Aldehydes and ketones.
b) Carboxylic acids and unsaturated compounds.
c) Amines and amides. d) All the above
e) None of the above

15
 Common data for questions 10 and 11
The urine sample of a hypertensive person who is on hydrochlorthiazide therapy
was analysed by liquid chromatography mass spectrometer.

10. Mechanism of action of hydrochlorthiazide is

a) Inhibit Na+-cl- symport b) Inhibit Na+-K+-2cl- symport
+ +
c) Inhibit Na -K antiport d) Inhibit Na+-H+ antiport

11. Urine sample mass spectra shows (M+) peak and (M+2)+ peak in the following
ratio
a) 1:3 b) 1:1
c) 3:1 d)1:2

12. Base peak observation in C6H6-CH2-R compounds is (m/z)

a) 90 b) 92
c) 94 d) 91

13. Toluene mass spectra shows base peak at 91 mass to charge ratio. This is
due to
a) Molecular ion b) Metastable ion
c) Daughter ion d) Tropylium ion

Common data for questions 14 and 15

Blood sample of Mr. Yashoda has analysed for determination of coumarin content
by LC-MS. The mass spectra shown a base peak at 118m/z(molecular
weight:146)

14. Which of the following is a coumarin derivative used as oral anticoagulant

a) Phenindiole b) Tromexan
c)Warfarin d) Both b and c
e) None of the above

15. The base peak at 118 due to loss from coumarin

a) C=O b) C2H4
c) –CH2-OH d) None of the above

16
 TARGET GATE
THE HYDERABAD ACADEMY
Name: Sub : Pharmaceutical Analysis
ID : Topic: Assays, NMR & ESR
Test : 7 Date :
1. Example for amphiprotic solvent
a) Methanol b) Glacial acetic acid
c) Water d) All of the above
e) None of the above

2. Nature of amphiprotic solvent

P) Acts as a base in strong acidic environment
Q) Acts as a acid in strong basic environment
R) Acts as a base in weak basic environment
S) Neutral in nature

a) P, R b) P, S c) Q, R d) P, Q

3. Which of the following weakly acidic drug analysed by 0.1 N Lithium

methoxide?
a) Caffeine b) Triamteren
c) Atropine d) β-Naphthol

4. Nature of acetic acid in non aqueous Perchloric acid titrations

a) Acid b) Base
c) Neutral d) None of the above

5. Principle involved in non aqueous titration of weakly basic drug

a) Proton donation from acetic acid to drug
b) Proton donation from onium ion to acetic acid
c) Proton donation from perchloric acid to acetic acid
d) All of the above
e) None of the above

Common data for question 6and7

“Premium pharmaceuticals was exporting propranolol HCl IP tablets .The
importing country had analysed the tablets as per IP”

6. Analysis technique used in assay of tablets

a) Non aqueous titration b) Aqueous acid base titration
c) Diazotization titration d) Redox titration

7. As nature propranolol HCl is weakly basic halogen acid salt, it needs addition of a
reagent for estimation
a) Lead acetate solution b) Mercuric acetate solution
c) Alkoxide solution d) None of the above

17
 Common data for 8and9
“A chemist in Roton pharmaceutical was analysing a multivitamin capsule
for the content of ascorbic acid as per IP” using ferroin in indicator.

8. Chemist should get the following end point

a) Bright red to pale blue b) Pale blue to red
c) red to blue c) Blue to red

9. Oxidation state of iron in ferroin indicator

a) Fe+2 b) Fe+3
c) Both a and b d) None

10. What are reduced components in the titration?

a) Ce and Fe b) Fe and ascorbic acid
c) Ce and ascorbic acid d) All of the above

11. Principle involved in estimation of Metronidazole from its tablet by

diazotization titration
a) Conversion of amine to diazonium salt b) Conversion of nitro to amine
c) Conversion of amine to nitro d) Both a and b
e) None of the above

12. For the assay of calcium lactate indicator used is

a) Erichrome black T b) Calcon mixture
c) Mordant black II d) Xylenol orange

13. Reason for the addition of magnesium sulphate solution in the assay of calcium
lactate
a) Magnesium EDTA complex is more stable than calcium EDTA
b) Calcium EDTA complex is more stable than magnesium EDTA
c) Magnesium indicator complex is more stable than calcium indicator
d) Calcium indicator complex is more stable than magnesium indicator

14. The following atom which exhibits NMR spectra.

a) C12 b) H1
b) N14 d) Both b and c
e) None of the above

15. Fate of spinning nucleus with the absorption of radiofrequency in the presence of
magnetic field.
a) Spin Alignment b) Spin-reversal
c) Both a and b d) None of the above

16. Magnetic field strength in H1 NMR is

a) 1.39 Tesla b) 1.4 Tesla
c) 1.5 Tesla d) None of the above

18
17. Solvent used for the analysis of H1NMR for aqueous soluble compound is
a) CDCl3 b) CCl4
c) DMSO-dc d) CD2Cl2

18. Different number of signals present in NMR spectra indicates

a) Different protons present in different environment
b) Different protons present in same environment
c) Different number of proton environment
d) Both a and b
e) None of the above

19. Chemical shift will be measured in

a) ppm b) δ (delta)
c) τ (tau) d) All of the above
e) None of the above

20. The phenomenon of induced magnetic field which opposes applied magnetic
Field is called
a) Shielding b) Deshielding
c) Coupling d) Decoupling

21. Match the following.

Compounds Number of signals

a) Acetone i) 01
b) Carbondisulphide ii) 03
c) Benzaldehyde iii) 04
d) Toulene iv) 02
v) 00

A) a-iii B) a-ii C) a-i D) a-iv

b-ii b-iii b-v b-i
c-iv c-i c-ii c-iii
d-i d-v d-iv d-ii

22. The order of shielding effect in CH3Cl, CH3Br, CH3F

a) CH3F > CH3Br > CH3Cl
b) CH3Br > CH3F > CH3Cl
c) CH3Br >CH3Cl > CH3F
d) CH3Br <CH3Cl<CH3F

23. The ratio of two signals present in toluene NMR spectra is

a) 3 : 5 b) 5 :3
c) 1 :3 d) 3 : 1

19
24. The following compound exhibits ESR spectra.
a) Diamagnetic b) Ferromagnetic
c) Paramagnetic d) None of the above

25. Conductance of semiconductors will be confirmed by

a) NMR b) IR
c) X –RD d) ESR

20
 TARGET GATE
THE HYDERABAD ACADEMY
Name: Sub : Pharmaceutical Analysis
ID : Topic: Gas Chromatography
Test : 8 Date :
1. To analyse the sample by GC, the nature of sample is.
a) Volatile and thermolabile b) Volatile and thermostable
c) Non-volatile and thermolabile d) Non-volatile and thermostable

2. The content of glycine from lysineacetylsalicylate with glycine injection can be

analysed by
a) GLC and GSC b) GSC and HPLC
c) GLC and HPLC d) None of the above

3. α-pinene is analysed by GC using the following detection in a preparative manner.

a) ECD b) FID
c) TCD d) ECD and TCD

4. Peak tailing in a GC chromatogram is due to

a) More absorption sites on stationary phase.
b) Less absorption sites on stationary phase.
c) Saturation of stationary phase.
d) Unsaturation of stationary phase.

5. The temperature program useful for efficient separation of complex mixtures by

GC is
a) Isothermal b) Linear
c) Non-linear d) None of the above

6. Which of the following detector is useful to detect the sample in nano gram
levels?
a) TCD b) FID
c) AID d) None of the above

7. Carboxylic acid and sugars can be analysed by GC by converting into less polar
nature through following technique.
a) Pre-column derivatization.
b) Post- column derivatization.
c) Online derivatization.
d) Both b and c

8. Efficiency of column in liquid chromatography can be improved by

a) Increasing number of theoretical plate.
b) Increasing HEPT.
c) Increasing flow rate.
d) All the above.

21
9. Differentiate analytic column from preparative column
a) More length and width.
b) Less length and more width.
c) More length and less width.
d) Less length and width.

10. Concentration of the analyte is determined in liquid chromatography based on

a) Area of the peak.
b) Height of the peak.
c) Retention time of the peak.
d) All the above.

11. The use of the septum devices in GC to inject.

a) Solid samples b) Liquid samples
c) Gases d) All the above

12. Fenfluramine tablets can be analysed by GC using following column.

a) Carbowax b) Phenylmethylsilicone
c) PEG column d) SCOT column

13. The most popular detection for quantitative analysis in GC.

a) FID b) AID
c) ECD d) TCD

14. Match the following

GC detector Sensitivity
a) FID i) 10-6
b) ECD ii) 10-11
c) TCD iii) 10-4
iv) 10-12
v) 10-9

A) a-i B) a-i C) a-ii D) a-iii

b-iii b-iv b-iv b-v
c-ii c-ii c-i c-iv

15. For the analysis of high molecular weight compounds the following GC technique
can be used
a. PGC b. GCMS
c. LCMS d. None of the above

22
 TARGET GATE
THE HYDERABAD ACADEMY
Name: Sub : Pharmaceutical Analysis
ID : Topic: HPLC
Test : 9 Date :

1. Most of the pharmacopoeial assays can be analysed by the following.

a) NP-HPLC b) RP-HPLC
c) HPTLC d) UPLC

2. Mode of the HPLC used for estimation of non-polar drugs.

a) NP-HPLC b) RP-HPLC
c) Chiral phase chromatography d) Both a and c

3. Estimation of levo-cetrizine from racemic mixture of cetrizine can be performed

with the use of
a) Ion exchange chromatography b) Chiral phase chromatography.
c) Ion-pair chromatography d) Both a and c

4. Chromatographic technique used in radio immunoassay.

a) Adsorption chromatography b) Affinity chromatography
c) Gel-permeation chromatography d) All the above

5. In RP-HPLC, as polarity of solvent increases what is the fate of analyte.

a) Increases b) Decreases
c) No change d) Moderate

Common data for question numbers 6, 7 and 8

Artemether injection is analysed by liquid chromatography with use of
• C18 column
• Mobile phase : ACN : H20 (75:25)
• Flow rate : 1.0 ml/min

6. Fate of retention time with increase in flow rate.

a) Increases b) Decreases
c) No effect d) No detection

7. Increase in polarity of mobile phase causes.

a) Increased column efficiency b) Increased number of theoretical plate
c) Decreased column efficiency d) Decreased retention time

8. Increased percentage of acetonitrile in mobile phase causes.

a) Increased retention time b) Decreased retention time.
c) Increases polarity of mobile phase d) No effect

23
9. Content of carbohydrates in a soft drink can be analysed.
a) RP-HPLC gradient b) RP-HPLC isocratic
c) NP-HPLC gradient d) NP-HPLC isocratic

Common data for question 10, 11, and 12

“Mixture of synthetic corticosteroids (prednisolone, hydrocortisone,
dexamethasone) were analysed using following conditions”
• Column-micro pak. Sil10
• Mobile phase-5% ethanol in dichloromethane
• Pressure-2000 psi
• Flow rate- 1-2ml/min
• λ max – 254 nm

10. Mode of HPLC is

a) Normal phase b) Reverse phase
c) Ion-exchange d) Gel permeation

11. First eluted drug (steroid)

a) Prednisolone b) Hydrocortisone
c) Dexamethasone d) Methyl prednisolone

12. Last eluted corticosteroid.

a) Prednisolone b) Hydrocortisone
c) Dexamethasone d) Methyl prednisolone

13. Detection used to sense all constituents of sample is

a) RI detector b) UV –visible detector
c) Fluorescent detector d) Circular dichrome detector.

14. Determination of sugar content of soyabean extract in RP-HPLC by using

following detector.
a) UV-detector b) RI-detector
c) Fluorescent detector d) None of the above

15. The refractive index detector is an example for

a) Bulk property detector b) Universal detector
c) Nonspecific detector d) All the above
d) None of the above

24
 TARGET GATE
THE HYDERABAD ACADEMY
Name: Sub : Pharmaceutical Analysis
ID : Topic: HPLC
Test : 10 Date :
1. High molecular weight compounds can be analysed by
a) HPLC b) Ion-exchange chromatography
c) Gel permeation chromatography d) Ion pair chromatography

2. Detector used in gel permeation chromatography

a) UV-detector b) Densitometric detector
c) Refractive index detector d) Differential refractometric detector

3. The gel used in analysis of trypsin by gel chromatography

a) Sephadex 75 b) Sephadex-25
c) Sephadex-50 d) All of the above

4. Composition of resin in Ion exchange chromatography

a) Polystyrene and vinyl benzene b) Polystyrene and divinyl benzene
c) Styrene and vinyl benzene d) Styrene and divinyl benzene

5. Match the following.

Match the type gel used in determination of compounds in gel permeation
Chromatography
GEL TYPE USE
a) Sephadex 75 i) Isomaltose and glucose
b) Sephadex 50 ii) Ribonuclease and trypsin
c) Sephadex 25 iii) Fructose and its derivatives
iv) Aminoacids
v) Separation of low molecular weight
molecules from high molecular weight
natural products
A) a-I B)a-v C) a-ii D)a-i
b-ii b-iii b-I b-iii
c-v c-v c-iv c-ii

6. Following drug can be analysed by ion-exchange chromatography.

a) Morphine b) Quinine
c) Bethamachol d) None of the above

7. Match the ion-exchange resins with respective to their PH ranges

Resins PH
a) Strong cation exchange resin i) 0-9
b)Week cation exchange resin ii)0-12
c) Strong anion exchange resin iii)5-14

25
 d) Week anion exchange resin iv)1-14
A) a-iv B) a-iii C) a-ii D) a-i
b-iii b-ii b-i b-iii
c-ii c-iv c-iv c-ii
d-i d-i d-iii d-iv

8. Match the following of composition of stationary phase in TLC

NAME COMPOSITION
a) Sillicagel G i) diatomaceous earth + binder
b) Silicagel-H ii) Silicagel without binder
c) Alumina iii) Silicagel + CaSO4
d) Kieselguhr iv) Al2O3 + Binder
v) Al2O3
A) a-i B) a-iii C) a-ii D) a-i
b-ii b-ii b-i b-iii
c-v c-iv c-iv c-ii
d-iii d-i d-iii d-iv

9. Edge effect is due to

a) Unsaturation of the development chamber b) Excess flow of solvent.
c) Both a and b d) None of the above.

10. Match the following detecting agents used in TLC with respect to drug
DETECTING AGENTS DRUG
a) Dragendroff’s i) GABA
b) Ninhydrin ii) Quinine sulphate
c) 2,4-DNP iii) Digoxin
d) 3,5-Dibutro benzoic acid iv) Steroids
A) a-ii B) a-iii C) a-ii D) a-i
b-i b-ii b-i b-iii
c-iv c-iv c-iv c-ii
d-iii d-i d-iii d-iv

11. Following destructive detection technique used in paper chromatography except

a) UV-chamber method b) Iodine chamber method
c) Both a & b d) specific spray method

12. What is the x-co ordinate in HPTLC chromatogram

a)R x b)R m c)R f d)None of the above

13. Principle involved in detectors used in HPTLC

a) Optical density b) Refractive index
c) Absorption of light d)None of the above

14. Most economical qualitative chromatographic technique used in pharma industry

a) HPTLC B) TLC

26
 C) HPLC D) None of the above
TEST NO-I KEYS
1.a 2. c 3. d 4. c 5. a 6. b
7. c 8. b 9. d 10. c 11. d 12. c
13. a 14. b 15. c 16. c

TEST NO-II KEYS

1. a 2. a 3.a 4. d 5. d 6.d
7. c 8. a 9. b 10. a 11. a 12. b
13. c 14.c 15. b 16. d 17. d 18. c
19. c 20.a 21.c 22.c 23.a 24.c
25.b

TEST NO-III KEYS

1.d 2.c 3.a 4.b 5.b 6.a
7.d 8.b 9. a 10. c 11. a 12. a
13. c 14. b 15. e 16. b 17. e 18.d
19. a 20.d 21.a 22.a 23.a

Test-IV KEYS:
1.d 2.a 3.b 4.a 5.b 6.d
7. b 8.b 9. c 10. a 11. a 12. d
13. d 14. e 15. c 16. b 17. b 18. d
19. c

TEST-V KEYS:
1.a 2.c 3.b 4.d 5.c 6.c
7.b 8.b 9.a 10.d 11.a 12.d
13.a 14.a 15.d 16.a 17.d 18.a
19.b 20.a

TEST-VI KEYS:
1.b 2.b 3.b 4.c 5.c 6.c
7.b 8.c 9.d 10.a 11.c 12.d
13.d 14.d 15.a

TEST-VII KEYS:
1. d 2. d 3. d 4. b 5. d 6. a
7. b 8. a 9. a 10. c 11. d 12. c
13. d 14. d 15. b 16. b 17. c 18. d
19. d 20. a 21. c 22. d 23. b 24. c
25. d

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TEST-VIII KEYS:
1.b 2. c 3. d 4. a 5. a 6. d
7. a 8. c 9. d 10. a 11. b 12. a
13. a 14. c 15.a

TEST-IX KEYS:
1.b 2. a 3. b 4. b 5. b 6.b
7. c 8. b 9. a 10. a 11. b 12.a
13. a 14. b 15. d

TEST-X KEYS:
1.c 2.d 3.a 4.b 5.a 6.c
7.a 8.b 9.c 10.a 11.d 12.c
13.a 14.b

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