0% found this document useful (0 votes)
125 views

Multiple Choice Questions (Numbers in Brackets Indicate The Number of Correct Answers)

The document contains multiple choice questions about various topics relating to genomes, including mitochondrial and chloroplast genomes, bacterial genome compaction, plasmids, operons, telomers, microsatellites, transposons, gene transcription, protein folding, restriction endonucleases, PCR, transformation, DNA polymerases, genome markers, linkage analysis, and physical mapping. It also lists self-study questions from chapters 2-5 of a GENOMES textbook.

Uploaded by

bmhsh
Copyright
© © All Rights Reserved
Available Formats
Download as DOC, PDF, TXT or read online on Scribd
0% found this document useful (0 votes)
125 views

Multiple Choice Questions (Numbers in Brackets Indicate The Number of Correct Answers)

The document contains multiple choice questions about various topics relating to genomes, including mitochondrial and chloroplast genomes, bacterial genome compaction, plasmids, operons, telomers, microsatellites, transposons, gene transcription, protein folding, restriction endonucleases, PCR, transformation, DNA polymerases, genome markers, linkage analysis, and physical mapping. It also lists self-study questions from chapters 2-5 of a GENOMES textbook.

Uploaded by

bmhsh
Copyright
© © All Rights Reserved
Available Formats
Download as DOC, PDF, TXT or read online on Scribd
You are on page 1/ 5

February 11, 2005

Multiple choice questions 
(numbers in brackets indicate the number of correct answers)

All mitochondrial genomes analyzed to date are
circular 
linear 
<50 kb in size 
>50 kb in size 
none of the above  (1)

All chloroplast genomes analyzed to date are
circular 
linear 
<50 kb in size 
>50 kb in size 
none of the above  (2)

Bacterial genomes are compacted by
Histones 
Other proteins than histones 
Supercoiling 
DNA polymerases 
DNA topoisomerases 
CP proteins  (3)

Plasmids are molecules that
are linear 
are circular   
are supercoiled in vivo 
are present in all bacteria 
contain essential genes 
replicate independently  (3)
Operons 
are characteristic for eukaryotic genomes 
contain more than one gene 
contain more than one promoter 
contain similar genes 
contain almost no intergenic sequences  (2)

Telomers are located
at the ends of ribosomal RNA 
in centromers 
in the middle of chromosomes 
at the ends of chromosomes 
in nuclear DNA 
in mitochondrial DNA 
in prokaryotes 
in eukaryotes  (3)

Microsatellites are
frequently found in bacterial genomes 
always smaller than 50 bp 
used as DNA markers 
repeated sequences 
movable DNA elements  (2)

Transposons
are RNA sequences 
are DNA sequences 
are only found in eukaryotes 
contain no genes 
contain at least one gene 
can replicate  (2)

Most genes are transcribed into
t­RNAs 
mRNAs 
ribosomal RNAs 
repeat elements 
small nuclear RNAs  (1)

Transcriptomes
consist of RNA   
consist of proteins 
are translated into proteins 
do not change   
differ in different cells  (3)

­sheets are stabilized by
hydrophobic bonds 
ionic bonds 
hydrogen bonds 
covalent bonds 
all of the above 
none of the above  (1)

Chaperons are involved in
translation 
transcription 
protein degradation 
polypeptide folding 
mRNA processing  (1)

Restriction endonucleases
are located in the nucleus 
degrade DNA completely 
bind to DNA 
are enzymes 
are proteins 
were discovered in the 1980s  (3)

PCR is used for
reverse transcribing RNA into DNA 
digesting proteins 
digesting DNA 
copying plasmids 
amplifying DNA 
amplifying proteins  (1)

Transformation
converts DNA into RNA 
converts RNA into proteins 
joins two DNA fragments 
cuts DNA into fragments 
introduces DNA into cells 
removes genomes from cells 
is used in cloning of DNA  (2)

DNA polymerases
join DNA fragments 
replicate RNA 
replicate DNA 
synthesize DNA in 5’­>3’ direction 
synthesize DNA in 3’­>5’ direction 
require a primer to function 
require nucleotides to function 
require ATP  (4)
Genome markers
Must occur as multiple alleles 
Must be repeat DNA sequences 
Can be any unique DNA sequence 
Are only used in genetic maps 
Are composed of foreign DNA  (1)

Linkage analysis
Is used in physical mapping 
Is based on Mendel’s laws 
Can only be used with prokaryotes 
Is based on recombination frequencies 
Requires unlinked genes  (2)

Physical mapping
Requires large numbers of organisms 
Is always based on optical methods 
Can use DNA fragment libraries 
Uses polymorphic restriction sites 
Can use sequence tagged sites (STSs)   (2)

Self study questions at the end of chapters 2, 3, 4, and 5 of the GENOMES textbook:

Chapter 2: 20, 22, 24.
Chapter 3: 2, 4, 11.
Chapter 4: 2, 4.
Chapter 5: 1, 3, 8, 10.

You might also like