Mohamed Neffati, Hanen Najja, Ákos Máthé (Ed.) - Medicinal and Aromatic Plants of The World - Africa Volume 3 (2017) PDF

Medicinal and Aromatic Plants of the World
Mohamed Neffati
Hanen Najjaa
Ákos Máthé Editors
Medicinal and
Aromatic Plants
of the World -
Africa Volume 3
123
Volume 3
Series Editor
Ákos Máthé
University of West Hungary
Faculty of Agriculture and Food Science
Mosonmagyaróvár, Hungary
More information about this series at http://www.springer.com/series/11192
Mohamed Neffati • Hanen Najjaa
Ákos Máthé
Editors
Medicinal and Aromatic

Plants of the World - Africa
Volume 3
Editors
Mohamed Neffati Hanen Najjaa
Arid Zone Research Institute Min of Agriculture, Range Ecology
Médenine, Tunisia Laboratory
Arid Zone Research Institute
Ákos Máthé Médenine, Tunisia
Faculty of Agriculture and Food Science
University of West Hungary
ISSN 2352-6831 ISSN 2352-684X (electronic)

ISBN 978-94-024-1119-5 ISBN 978-94-024-1120-1 (eBook)
DOI 10.1007/978-94-024-1120-1
Library of Congress Control Number: 2017944445
© Springer Science+Business Media B.V. 2017

This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of
the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation,
broadcasting, reproduction on microfilms or in any other physical way, and transmission or information
storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology
now known or hereafter developed.
The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication
does not imply, even in the absence of a specific statement, that such names are exempt from the relevant
protective laws and regulations and therefore free for general use.
The publisher, the authors and the editors are safe to assume that the advice and information in this book
are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the
editors give a warranty, express or implied, with respect to the material contained herein or for any errors
or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims
in published maps and institutional affiliations.
Printed on acid-free paper
This Springer imprint is published by Springer Nature

The registered company is Springer Science+Business Media B.V.
The registered company address is: Van Godewijckstraat 30, 3311 GX Dordrecht, The Netherlands
Foreword
The utilization of medicinal and aromatic plants (MAPs) dates back to the b eginnings
of mankind. This fact is increasingly true in the case of Africa, a continent that is
considered to be the cradle of mankind.
The rich and diverse flora of Africa has been basis/home for the evolution of
African traditional medicine. This holistic discipline incorporates the use of natural
plant resources (in the form of indigenous herbalism) as well as African spirituality.
In the course of millennia, through the ages, the use of MAPs has also seen extreme
development, so that to date, the most sophisticated medicines are already available
on the African continent as well. Nonetheless, healing with medicinal plants has
retained its central place in the life of the masses. Beyond traditions, this can be
attributed to the fact that synthetic medicines are still simply not affordable for
many.
Scientific progress has brought about a progress also in the use of traditional
medicinal plants. This means that the medicinal and aromatic plants historically
used by the practitioners of traditional African medicine (diviners, midwives, herb-
alists) are being gradually subjected to scientific investigations. Modern pharmacy
wants to isolate and utilize their active principles in the form of modern preparation
forms that – in turn – can be rendered accessible to consumers already on a global
scale.
As a result of this briefly (schematically) described process, the MAP resources –
formerly used only by the local people – must come up to cover global demands.
Obviously, this is not possible without farther protection, sustainable management
and, as an ultimate option, cultivation.
In view of the intensive R+D activities into African MAPs, the special literature
abounds in publications. Although even the present pace of research is not consid-
ered as sufficient by many, from handbooks to clinical research papers, the number
of both comprehensive and specific publications related to MAPs is imposing.
Volume 3 distinguishes itself from most available volumes on African MAPs by
avoiding the semblance of completeness. Squeezing all knowledge into one volume
would be absolutely impossible.
v
vi Foreword
Starting out from an overview of the status of MAPs in Africa, two introductory
chapters offer most comprehensive, valuable reviews of most important African
MAPs, as well as their traditional uses. The chapters to follow are compilations of
up-to-date information on selected characteristic African MAP species (e.g. African
cherry, bird plum), geographic regions (East and North Africa, Mauritius,
sub-Saharan Africa, Morocco, Nigeria, Tunisia) or MAPs growing under special
ecological conditions (arid and desert zones) and in cultivation, in Nigeria. In most
contributions the utilization, botanical, phytochemical as well as pharmacological
properties/traditional medicinal uses, of relevant species will be discussed.
It is unhidden hope of the author’s collective that the volume will serve as a use-
ful and updated complement to what has already been achieved in summarizing and
publishing basic knowledge on African medicinal and aromatic plants.
Médenine, Tunisia Mohamed Neffati

Mosonmagyaróvár, Hungary Ákos Máthé
Médenine, Tunisia Hanen Najjaa
Contents
I ntroduction to Medicinal and Aromatic Plants in Africa........................... 1

Ákos Máthé, Mohamed Neffati, and Hanen Najjaa
Review of African Medicinal and Aromatic Plants.................................. 19
A
Ben-Erik Van Wyk
raditional Use of Medicinal and Aromatic Plants in Africa...................... 61
T
Olufunke O. Fajinmi, Olaoluwa O. Olarewaju, and J. Van Staden
edicinal and Aromatic Plants of the World – Africa................................. 77
M
Ossy M. J. Kasilo, Mawuli Kofi-Tsekpo, and Francis Gachathi
ome Important Aromatic and Medicinal Plants of Morocco..................... 91
S
Saadia Zrira
runus africana (Hook. f.) Kalkman (the African Cherry).......................... 127
P
Mélissa Rousseau, Claire Delvaux, Ezekiel Edward Mwakalukwa,
Lawrence Mbwambo, Marie Caroline Momo Solefack, Harisoa Bako
Ravaomanalina, Coert J. Geldenhuys, Ntamwira Niranda Seintsheng,
Nils Bourland, and Hans Beeckman
Bird Plum; Berchemia discolor (Klotzsch) Hemsl.: A Review
of Its Ethnobotany, Phytochemistry and Pharmacology.............................. 143
Ahmad Cheikhyoussef and Alfred Maroyi
romatic and Medicinal Plants of Tunisian Arid and Desert Zone
A
Used in Traditional Medicine, for Drug Discovery
and Biotechnological Applications.................................................................. 157
Hanen Najjaa, Abdelkarim Ben Arfa, Ákos Máthé, and Mohamed Neffati
romising Indigenous and Endemic Medicinal Plants
P
from Mauritius................................................................................................. 231
Mohamad Fawzi Mahomoodally and Muhammad Zakariyyah Aumeeruddy
vii
viii Contents
ome Wild Herbaceous Plants of Nigeria: A Biological,

S
Pharmacognostic and Phytochemical Review............................................... 249
Sunday E. Atawodi, Mubarak L. Liman, Olufunsho D. Olowoniyi,
and Godwin O. Adejo
hytochemical, Pharmacological and Therapeutic Potentials
P
of Some Wild Nigerian Medicinal Trees......................................................... 283
Sunday E. Atawodi, Olufunsho D. Olowoniyi, Godwin O. Adejo,
and Mubarak L. Liman
iological, Pharmacognostic and Phytochemical Review
B
of Some Cultivated Medicinal Plants of Nigeria........................................... 311
Sunday E. Atawodi, Godwin O. Adejo, Olufunsho D. Olowoniyi,
he Halophytic Genus Zygophyllum and Nitraria from North
T
Africa: A Phytochemical and Pharmacological Overview........................... 345
Soumaya Bourgou, Wided Megdiche, and Riadh Ksouri
I n vivo Anticandida Activity of Three Traditionally Used Medicinal
Plants in East Africa........................................................................................ 357
D.P. Kisangau, K.M. Hosea, H.V.M. Lyaruu, C.C. Josep,
Z.H. Mbwambo, and P.J. Masimba
Wild Edible Allium Species from Arid Zones of Tunisia:
New Perspectives of Natural Compounds...................................................... 375
Hanen Najjaa, Abelkarim Ben Arfa, and Mohamed Neffati
tilization of Pteridophytes as Herbal Medicines
U
in Sub-Saharan Africa..................................................................................... 383
Alfred Maroyi
Index.................................................................................................................. 409
Introduction to Medicinal and Aromatic
Plants in Africa
Ákos Máthé, Mohamed Neffati, and Hanen Najjaa
Abstract Natural substances of plant origin, have been used by man from the very
early times of civilizations. Traditional Medicine (TM), a heritage of Africa, is still
servicing approx. 80% of the population of this huge continent comprizing 55 coun-
tries, 800 languages and an unmeasurable wealth of plant diversity. The continent
with a varied flora of an estimated 68,000 plant species is home to about 35,000
endemic species (Cunningham A, African medicinal plants. Setting priorities at the
interface between conservation. UNESCO, Paris, 1993), growing in seven centers
of endemism. Africa’s not yet fully explored medicinal plant resources frequently
come into the forefront of interest. The recent upsurge is witnessed by the fact that
ca. 60% of all recent publications on African medicinal plants, appeared during the
last decade. There is a tremendous potential for developing new crops and new
products. Research into MAPs seem to offer a nearly unlimited scope for explora-
tion. The ongoing screening for the biological activity of MAPs is also broad and
covers several important health related aspects including antimalarial and other
antiprotozoal products, anti-proliferative potential, anti-inflammatory and analgesic
activities, anti-diabetes activity antioxidant activity and activity on the reproductive
cardiovascular and central nervous systems, etc. Despite of this marked recent
increase, Africa is lagging behind Europe and Asia in terms of the number of prod-
ucts that have been commercialized and the percentage of flora utilized for interna-
tional trade. The growing demand on resources, however, has resulted, in some
cases, the overexploitation, the occasional local disappearance of favored sources of
botanicals and have reduced the diversity of species. By elaborating international
and national standards of sustainability, these dangers should be either eased or
eradicated. Domestication of wild-crafted species can also contribute to preserve
the genetic resources and to reduce the sometimes imminent threats of extinction.
The recent initiative to develop national and ultimately an African Herbal
Á. Máthé (*)
Faculty of Agriculture and Food Science, University of West Hungary,
e-mail: [email protected]
M. Neffati • H. Najjaa
Arid Zone Research Institute, 4119 Médenine, Tunisia
e-mail: [email protected]; [email protected]
© Springer Science+Business Media B.V. 2017 1

M. Neffati et al. (eds.), Medicinal and Aromatic Plants of the World - Africa
Volume 3, Medicinal and Aromatic Plants of the World,
DOI 10.1007/978-94-024-1120-1_1
2 Á. Máthé et al.
Pharmacopoeia is likely to mark an important milestone in meeting quality, safety

and efficacy standards both in African and international perspectives.
Keywords Medicinal and aromatic plants • Traditional medicine • African flora •

Biodiversity hotspots • Sustainable management • Cultivation • Pharmacopoeia
1 Introduction
Africa is the world’s second largest continent after Asia, both in terms of area and
population. In 2010, its entire population was estimated at 1010.3 million people
(FAO 2012). The African continent comprises ca. 55 countries, 800 languages, 3000
dialects, and as such it is a veritable treasure of genetic resources including medici-
nal plants (Simon et al. 2007). More than half of Africa’s population makes a living
from agriculture (FAO 2012). Medicinal plants are still widely used in the health-
care systems of Africa, particularly by the African population.
According to Mander and Le Breton (2005), alone in southern Africa, there are
up to 100 million traditional-remedy consumers and ca. 500,000 traditional healers.
Some 700,000 tons of plant material is consumed annually with an estimated value
of as much as 150 million US dollars per annum.
Medicinal plants are often a basic requirement for the treatment of certain condi-
tions irrespective of education and income levels of the patient (Cocks and Dold
2000). The use of traditional medicines is not confined to rural, low-income groups,
but also prevails in urban areas.
The trade in traditional medicines (TM) forms part of a multimillion-Rand ‘hid-
den economy’ in southern Africa (Cunningham 1989). It is stimulated by high pop-
ulation growth rates, rapid urbanization and the important cultural value placed on
traditional medicines, so that this trade is now greater than at any time in the past
(Mander et al. 2006).
The African-continent has an important role as a source of medicinal and aro-
matic plants for the world’s food, drug, herb, dietary supplement, and cosmetics
markets. Although, in changing supplies, numerous African raw plant materials still
have an established, strong international market presence. People around the world
enjoy Africa’s manifold “culinary contributions” including the peanut (Arachis
hypogaea, Fabaceae), yam (Dioscorea spp., Dioscoreaceae), watermelon (Citrullus
lanatus, Cucurbitaceae), okra (Abelmoschus esculentus, Malvaceae), as well as
numerous other foods and flavors like coffee (Coffea spp., Rubiaceae) (Blumenthal
2011).
This wealth of plant species has been subject to a multitude of scientific investi-
gations that also reveal the versatility of international contributions Africa’s. As
such, the number of references on “African MAPs” in the scientific database
SCOPUS exceeds 25,000, in the period 2010–2015 and it seems to be farther
increasing at a steady pace (e.g. ca. 9330 entries, in the current year of 2016).
Introduction to Medicinal and Aromatic Plants in Africa 3
Fig. 1 Terrestrial ecoregions of the world: 14 biomes and eight biogeographic realms (Olson et al.
2001)
Remarkably, despite of the relevant dynamic increase in the number of publica-

tions on indigenous medicinal plants and dietary supplements, Africa is lagging
behind Europe and Asia. Similarly, a relatively low percentage of the African flora
has been utilized for international trade (Van Wyk 2015). So, Africa remains to be a
continent with nearly unlimited potentials for research and development in the
exploration of new MAP crops and new products.
Africa as a Botanical Continent
According to the World Geographical Scheme for Recording Plant Distributions

(WGSRPD) (Brummitt and Pando 2001), which provides definitions and codes for
recording plant distributions, Africa is a botanical continent that corresponds
closely to the usual geographical definition. It excludes the Sinai Peninsula, which
is placed in the region of Western Asia. To the west, it includes islands grouped as
Macaronesia, comprising the Azores, the Canary Islands, and the Cape Verde
Islands. To the east, it includes Madagascar and other Indian Ocean islands out as
far as the island of Rodrigues (Udvardy 1975) and (Brummitt et al. 2001) (Fig. 1).
Table 1 Biodiversity hotspots in Africa

Plant species Endemic species
Hotspot Number
Eastern Arc and Coastal Forests of 4000 1500
Tanzania/Kenya
West African Forests 9000 2250
Cape Floristic Region 8200 5682
Succulent Karoo 4849 1940
Madagascar 12,000 8704
After Myers et al. (2000)
Biodiversity Hotspots of Africa
The concept of “hotspots” has been elaborated to denote those natural areas that are
characterized by exceptional concentrations of species with high levels of ende-
mism and are experiencing unusually rapid rates of depletion (Myers 1988) and
(Klopper et al. 2002). On a worldwide scale, 44% of all vascular plant species are
confined to 25 recognized hotspots. This amounts to merely 1.4% of the Earth’s ter-
restrial surface. Four out of the 25 hotspots can be found on the African continent
and an additional one is Madagascar (Table 1).
According to Conservation International (Anonymous 2005) biodiversity
hotspots, the Earth’s biologically richest places with high numbers of endemic spe-
cies, face extreme threats and have already lost at least 70% of their original
vegetation.
The overexploitation of Africa’s natural resources has also frequently lead to the
destruction of both natural populations and vast stretches of unique natural areas. As
habitat loss is often accompanied by the extinction of equally unique organisms and
ecosystems, ultimately, the rich and unique biological heritage of the African conti-
nent can be at stake. In the lack of exact and comprehensive documentation of both
extent and threatened species, this process can be regarded as greatly threatening
(Klopper et al. 2002).
Flora of Africa
The African continent has a unique diversity of geographic and climatic factors. It
has a similarly exceptionally rich and varied flora with an estimated 68,000 plant
species, of which about 35,000 are known to be endemic (Cunningham 1993).
Exceptionally rich in endemic species (8500 species, each) are the Guineo-
Congolian and Cape biogeographic units. The latter is known as the Cape Floristic
Region and encompasses five of the South Africa’s 12 endemic plant families. The
Cape region has predominantly Mediterranean type of vegetation (Van Wyk et al.
2009). On the other hand, the Guineo-Congolian region can be ranked to the African
Table 2 Seven centers of endemism in Africa

Plants
Biogeographic unit Area 1000 km2 No. of species %
1. Guineo-Congolian 2815 8000 80
2. Zambesian 3939 8500 54
3. Sudanian 3565 2750 33
4. Somali-Masai 1990 2500 50
5. Cape 90 8500 80
6. Karoo-Namib 629 3500 50
7. Afro-montane 647 3000 75
After Cunningham (1993)
rain forests. Lowest frequency of endemism has been explored in the Somali-Masai
floristic region, a mostly arid semi-desert grassland and scrubland (White 1983).
The main geographical units of plant endemisms, according to Cunningham (1993)
are summarized in Table 2.
onservation and Sustainable Management of MAP Resources

C
in Africa
The sustainable management of traditional medicinal plant resources has become an

important issue, not only because of the value of the natural flora as a potential
source of new drugs (Elujoba et al. 2005), but also due to population growth accom-
panied by the significant reliance, in many African countries, on the use of tradi-
tional medicinal plants for health. The indiscriminate harvesting of spontaneous
flora, including forests has already resulted in severe losses in habitats and genetic
diversity. Additional sources of threat are forest clearance for agriculture, afforesta-
tion of montane grasslands, uncontrolled burning and livestock grazing. Frequently,
it is the exclusion from core conservation areas that affects medicinal plant supplies
adversely. The effect of competing resource uses (e.g.: as timber logging, commer-
cial harvesting for export and extraction of pharmaceuticals, and use for building
materials and fuel) is similar.
In brief, a growing demand for fewer resources has resulted, in some cases, the
local disappearance of favored and effective sources of botanicals that have lead to
reduced species diversity. In this process, the popular, slow growing or slow to prop-
agate species or species with specific habitat requirements and a limited distribution
are most vulnerable (Cunningham 1993).
In 2010, over 2000 plant species, about 1000 species of mammals and nearly
2000 species of fish were threatened, in Africa, whereas globally, in the same year,
the relevant numbers were: more than 10,000 plant species, about 6000 species of
fish and over 3000 species of mammals (FAO 2012).
Fig. 2 Endangered plant species in Africa (IndexMundi 2016)
A country-wise statistic of endangered species reveals that the African country

with the highest value of 496 species, is Tanzania and the country with the lowest
value of 0, is Mauritania (IndexMundi 2016) (Fig. 2).
As a part of natural diversity, the above described trends in the overexploitation
of natural resources, have already lead to substantial losses in African MAP
resources. The importance of medicinal plants in primary health care, both in self-
medication and in national health services was first recognized by participants of the
WHO/IUCN/WWF International Consultation on Conservation of Medicinal
Plants, held in Chiang Mai, 21–26 March 1988 (Van Seters 1997).
Alarmed by the consequences of loss of plant diversity around the world, the so
called Chiang Mai Declaration was issued under the motto: “Saving Lives by Saving
Plants”. The Declaration affirms the importance of medicinal plants and calls on the
United Nations, its agencies and Member States, as well as other international orga-
nizations, to take action for the conservation and sustainable use of medicinal
plants (Máthé 2015).
Documents by year
250
200
Documents
150
100
50
0
1931 1939 1947 1955 1963 1971 1979 1987 1995 2003 2011
Fig. 3 Number of SCOPUS documents of the search “medicinal plants Africa” for the period
1930–2015
aving Plants that Save Lives: Effect of Chiang Mai Declaration

S
(1988)
The belated growth in international awareness about the declining supply capacity
of MAPs, the over-harvesting of natural resources, the destructive harvesting prac-
tices accompanied by habitat loss, forest degradation, etc. are apparent, also on the
African continent.
It seems that the Chiang Mai Declaration (1988) calling for coordinated interna-
tional efforts to safeguard the natural populations of plant biodiversity has had a
spurring effect on the study of African medicinal plants, too.
Our survey of the relevant special literature of the decades following the
Declaration have revealed the emergence of new terms previously not used by the
scientific community. E.g. the term “exploitation of MAPs” did not figure in the
SCOPUS databases of special literature, until 1998 (Fig. 3).
Pharmacology, toxicology and pharmaceutics (788), Agricultural and Biological
Sciences (634), as well as Medicine (601) have been the most popular scientific
areas on African MAPS with entries mostly from South Africa (752), Nigeria (160),
United States (139), Cameroon (105) and the United Kingdom (100).
As regards biodiversity and conservation of medicinal and aromatic plants in
Africa, some important contributions have opened up a comprehensive insight into
both problems and possibilities (Okigbo and Mmeka 2006; Makunga et al. 2008;
Rukangira 2001; Dzoyem and Tshikalange 2013), etc. These authors also empha-
size the importance of saving Africa’s medicinal plant resources from extinction and
call for intensive management as well as conservation, more research and increased
level of public awareness about this vanishing African heritage. Effective
Table 3 Spice and herb export (in US $) of 10 major exporting countries

Exported value (US $)
Exporters 2011 2012 2013 2014
World 8,066,358 5,611,580 4,847,018 5,793,174
Africa Aggregation 534,619 487,354 464,563 512,673
Madagascar 180,362 185,462 135,84 247,714
South Africa 64,444 64,429 66,741 70,229
Tanzania (United Republic of) 31,912 3817 43,283 45,627
Comoros 17,628 10,348 7836 33,57
Nigeria 42,285 88,537 9863 33,134
Morocco 17,925 17,158 22,744 28,487
Ethiopia 38,897 31,377 31,238 2257
Kenya 11,146 12,976 674
Mauritius 1538 4172 1731 5065
Uganda 922 5581 529 382
Tunisia 31,961 2878 306 3636
Egypt 33,144 27,336
After ITC Trade statistics (2016)
conservation strategies are needed especially in the following four main areas:
in-situ and ex-situ conservation, education and research.
Ultimately, the future of medicinal and aromatic plants in Africa seem to depend
on the ability to resolve the current conflicts between conservation and resource use,
as well as a shift towards more resource based agriculture that is already being chal-
lenged by the globalization of economics (Okigbo et al. 2008).
Commercial Potential of African MAPs
Africa is a continent that not only possesses a vast treasure of medicinal plants but
also produces some exclusive materials for the world market.
A more recent statistics, as available from the International Trade Center (Market
Analysis and Research n.d.), indicates that in terms of sales values Madagascar,
South Africa and Tanzania were the three main exporters countries (Table 3). These
countries preserved their leading place also in the four consecutive years of analy-
sis. In these years, the exports showed an increasing trend, with the exception of
Madagascar, where a set-back was experienced, in the year 2013. As a contrast, the
aggregate spice and herbs exports from Africa have seen a slight drop back, which
has been the case also with the World figures (Market Analysis and Research n.d.;
Profound Advisers in Development 2014; Dzoyem et al. 2013).
In the lack of exact assessments, the importance of this special economic botani-
cal group of plants has been demonstrated in an indirect way by van Wyk (2015).
His method was to evaluate the relevant search results in SCOPUS (Elsevier), the
largest abstract and citation database of peer-reviewed scientific journals, books and
conference proceedings. On the basis of a “strongly focused inventory of species”
that was meant to cover most of the medicinal plant raw materials traded interna-
tionally (i.e.: exported from African countries) some informative observations/con-
clusions have been made.
It has been established that more than some 5400 plant species are used in tradi-
tional medicine (TM), in Africa. Less than 10% of these have been commercially
developed to some extent. Africa can be regarded as home to more than 80 valuable
commercial species that are regularly traded in international markets. These include
phytomedicines (e.g. Harpagophytum procumbens and Pelargonium sidoides),
functional foods (e.g. Adansonia digitata and Hibiscus sabdariffa) and sources of
pure chemical entities (e.g. caffeine from Coffea arabica and yohimbine from
Pausinystalia johimbe) (Van Wyk 2015).
Chemical and Pharmacological Potential of African MAPs
Several African species have been found to contain active principles that can have a
great potential in the fight against several global health issues. Examples are mani-
fold. African medicinal plants of the Families Moraceae, Guttiferae, Fabaceae, etc.
have been found to offer good alternative treatment against cancer which has been
recognized as a critical health problem in Africa, too (Kuete 2013).
In terms of pharmacological activities, African MAPs are also manifold. They
have been widely studied according to their following activities: Antimalarial and
Other Antiprotozoal Products, Anti-proliferative Potential, Anti-inflammatory and
Analgesic Activities, Anti-diabetes Activity, Antioxidant Activity and activity on the
Reproductive Cardiovascular and Central Nervous Systems (Atawodi 2005; Fennell
et al. 2004; Juliani et al. 2007; Amoa Onguéné et al. 2013; Van Wyk 2011; McGaw
et al. 2014; Kuete et al. 2013; Tekwu et al. 2013).
Cultivation of MAPs in Africa: A New Dimension in Agriculture
To-date, wild-crafting (collection) is still the main ways of harvesting medicinal

plants in Africa. However, as earlier discussed, this has a significant detrimental
impact on both the ecosystem and the survival of plant species.
Cultivation of highly valued medicinal plants is therefore expected to create new
dimensions in the field of agriculture also for the developing African nations.
Elaborate food-crop production technologies and techniques can be deployed in
cultivation programs that could ultimately contribute to improving also the medici-
nal value of plant species.
According to Wiersum and Shackleton (2005) there exist a wide array of avail-
able plant production systems and different degrees of (semi)-domestication of wild
plants.
It should be also noted that frequently the domestication process is inferred in
context with the modification of morphological characteristics and genetic make-up
of plants, as well as the “artificialization” of the biophysical environment in which
the plant is growing (Wiersum et al. 2006). This concept has been also interpreted
as to include the “acculturalization” of a plant to a given social management envi-
ronment (Wiersum 1997). According to this interpretation, the stimulation process
involves a socio-technical approach in which attention is given to the interaction
between technical, cultural and institutional dimensions of domestication, i.e.: tech-
nology development is not a value-free process, but rather a location-specific devel-
opment process in which cultivation practices are developed on the basis of both
technical options, socio-cultural conditions and specific livelihood conditions of the
intended cultivators.
This, in view of the development needs of underprivileged groups of people,
means that the technologies to be elaborated should be adjusted to the specific social
values and livelihood assets of the intended target groups.
ultivation in Farming Systems vs. Sustainable Exploitation

C
of MAPs
In Africa, much attention has been given to the possibility of combining biodiversity
conservation and poverty alleviation.
In an overview of medicinal commercial plant harvesting and its relevance to
medicinal plant conservation and the self-sufficiency of traditional medical practi-
tioners, Cunningham (1997) emphasized that generally, the most vulnerable species
are the popular, slow reproducing species with specific habitat requirements and
limited distribution. In most African countries, the sustainable use of barks, roots or
whole plants used as herbal medicines is, in theory, possible, still due to the high
level of input of resources (e.g.: money and manpower required for intensive man-
agement of slow-growing species) in multiple-species systems is likely to be rather
limited,. It seems, therefore, necessary to secure alternative supply sources for pop-
ular, high conservation priority species outside of the core conservation areas. In the
core conservation areas, however, the slow-growing species constitute a priority for
both ex-situ conservation and strict protection. As for some species high prices can
be achieved, they make potential new crop plants, either for agroforestry systems
(e.g. Warburgia salutaris, Garcinia kola, G. afzellii, G. epunctata) or agricultural
production (e.g. Siphonochilus aethiopicus).
This trend has received a strong impetus, especially, since the Johannesburg
Earth Summit, in 2002. According to Wiersum et al. (2006), in this regards, two
options merit special attention: i.e. the stimulation of sustainable exploitation of
wild growing plant resources (e.g. in natural forests) or the cultivation in farming
systems.
The way people use and manage wild plant resources will significantly influence
the sustainability of their livelihoods and the conservation of diversity (Canter et al.
2005).
The Example of Prunus africana
The bark of African cherry (Prunus africana (Hook. f.) Kalm.) has been used, for
the last 35 years, in the treatment of benign prostatic hyperplasia and other disorders
(Stewart 2003). The bark used for the preparation of Pygeum (bark extract used in
the treatments) takes its origin entirely from wild-collection. Major exporting coun-
tries include Cameroon, Madagascar, Equatorial Guinea, and Kenya. Global exports
of dried bark in 2000 have been estimated at 1350–1525 tons per annum, with a
peak of 3225 tons, in 1997.
The natural occurrence of Prunus africana ranges from east to west and from
central to southern Africa, posing several, different threats to the species. According
to (Jimu 2011) P. africana populations in west (Cameroon and Equatorial Guinea)
and east (Kenya, DRC, Uganda), as well as the island of Madagascar are threatened
mainly by commercial harvesting, whereas in southern Africa the main threat is
caused by habitat degradation/fragmentation accompanied by invasive alien species
and traditional wild-harvesting for medicinal purposes (The latter are also common
in west and east Africa). Climate change is likely to affect the species throughout
its range of area, especially in the lack of measures to ensure the adaptation of this
species. All these factors call for country or region specific conservation
strategies.
Since P. africana occurs in ecologically important habitats, i.e. the afromontane
forests that are rich in endemic, endangered and rare fauna and flora, the focus
should be on the conservation of these ecosystems.
Sustainable harvesting should be promoted together with other conservation
strategies (e.g. in-situ, circa-situ and ex-situ conservation): the ultimate aim should
be to avoid/reduce both habitat destruction and destructive wildfires.
Since 1995, Prunus africana has been included in CITES (the Convention on
International Trade in Endangered Species of Wild Fauna and Flora) Appendix II as
an endangered species (Cunningham 1997).
As a result, various project initiatives have been launched to elaborate alterna-
tives to wild-collection while also meeting future market demands: these include
conservation practices, enrichment plantings, small- and large-scale production,
and protection of genetic resources. There is hope that the status of this species will
shift from the verge of transition of an exclusively wild-collected species to that of
a cultivated medicinal tree (Stewart 2003).
Organic Production of MAPs in Africa
Growing international organic markets seem to stimulate organic production also in

Africa. Several non-governmental organizations (NGOs) and development partners
have designed and supported projects aimed at increasing farmers’ incomes by tap-
ping into the opportunities offered by organic production. Many farmers produce
organically by default, making it relatively easy to convert to true organic agricul-
tural practices. Smallholders, however, often do not have access to international
markets.
As a rule, already available certifications, e.g. Kilimohai (East African Organic
Products Standard) used by them regionally, are not yet approved at the interna-
tional markets. The East African organic products standard by TOAM (Tanzania
Organic Agriculture Movement) is mostly used for self-assessment by producers,
declarations of conformity in the marketplace, certification by certification bodies
in the region, or other kinds of verification. If the standard is used for the purposes
of third-party certification, inspection and certification should be carried out in
accordance with international norms, such as ISO Guide 65 or the IFOAM
Accreditation Criteria. If adherence to the standard is verified through other mecha-
nisms, those mechanisms shall adhere to the principles of competency, integrity and
transparency (TOAM 2016). Generally, most individual buyers are still not able to
meet the quality and quantity requirements of international buyers, however, there
are positive examples, e.g. Tanzania Organic Agriculture Movement (TOAM) coor-
dinates and promotes the development of the organic farming sector among small-
holder producers, in order to realize sustainable livelihoods in Tanzania (Barrett
et al. 2001; Bakewell-Stone et al. 2008; Profound Advisers in Development 2014).
MAPs in Traditional Medicine: A Heritage in Africa
African medicinal plant resources are abundant. A recent volume by Iwu (Iwu 2014)
provides a comprehensive review of more than 2000 species of plants deployed in
indigenous African medicine, with references from over 1100 publications.
Despite of their vast natural resources, most African countries are still struggling
to provide basic amenities for their people. Health facilities are deplorable and most
of the population continues to rely on traditional remedies derived from medicinal
plants. In consequence, Traditional African Medicine (TAM) as a socio-economic
and socio-cultural heritage, is still servicing over 80% of the populations in Africa
(Elujoba et al. 2005).
As a, quasi, international ramification (impact) of African TAM, quite a number
of African medicinal herbs have become increasingly popular, recently as dietary
supplements, food supplements, natural health products, therapeutic goods, tradi-
tional medicines, and even conventional drugs (obviously, only in harmony with the
regulatory system of the relevant countries). These herbs, according to Blumenthal
(2011) include the controversial diet aid hoodia (Hoodia gordonii, Asclepiadaceae),
the antioxidant red tea rooibos (Aspalathus linearis, Fabaceae), the anti-malarial
cryptolepis (Cryptolepis sanguinolenta, Asclepiadaceae), the 5-hydroxy-L-
tryptophan-containing griffonia (Griffonia simplicifolia, Fabaceae), anti-
inflammatory and analgesic devil’s claw (Harpagophytum procumbens,
Pedialiaceae), sausage tree (Kigelia africana, Bignoniaceae), the anti-tonsillitis and
anti-bronchitis umckaloabo (Pelargonium sidoides, Geraniaceae), the difficult-to-
sustain prostate aid pygeum (Prunus africana, Rosaceae; syn. Pygeum africanum),
the anti-depressive and mood-enhancing sceletium (Sceletium tortuosum,
Aizoaceae), and many others.
The current upsurge of interest in herbal medicines all over the world had lead to
the unregulated exploitation of Africa’s bio-resources, is a threat to the extinction of
plant species, while the traditional healers themselves are hopelessly being observed
to advance in age and gradually disappearing from the surface of the earth. Therefore,
traditional medicine knowledge should be learned, acquired, documented and used
for the benefit of both African people and the entire mankind (Elujoba et al. 2005).
frican Herbal Pharmacopoeia and Research to Promote

A
the Medicinal Plant Sector
With the increased popularity of medicinal and aromatic plants, an inevitable need
has emerged for enhanced quality control of raw materials, including tests for
botanical identity and potential – accidental or intentional – adulterants. The present
volume is expected to contribute to the medicinal plant sector in African countries
by offering complementary, comprehensive information on a wide range of medici-
nal and aromatic plants.
There have been various resolutions adopted by the World Health Assembly and
Regional Committee for Africa that call upon Member States, to develop herbal
pharmacopoeias and to develop/apply scientific criteria and methods for proof of
safety and efficacy of medicinal plant products. However, only few countries have
developed national herbal pharmacopoeias. The number of plant species with suf-
ficient information on the scientific (clinical) evaluation of their herbs, their possi-
ble medical applications, as well as the safety and efficacy of their usage is relatively
rather scarce. Without well documented information on safety, efficacy and phyto-
chemical characteristics, it is difficult to assess the virtual value of some new raw
materials and extracts of African origin. In order to address these shortcomings, the
Association of African Medicinal Plants Standards is developing an African Herbal
Pharmacopeia with trading standards which provide information and technical data
on some 50 important medicinal plants (Gurib-Fakim and Kasilo 2010).
The objective of developing these monographs is to ensure that these plants
become visible on the world market. The monographs lay emphasis on the quality
control issues, dosage, use, efficacy, pharmacology and safety of important African
medicinal plants. Despite the increasing popularity of phytomedicines of African

origin, the number of publications dealing with the results of controlled clinical tri-
als is relatively modest. Consequently, the African pharmacopoeia initiative can be
rightly regarded as the first African, interdisciplinary effort to compile information
in the form of a pharmacopeia. Ultimately, a reliable, authoritative compendium of
standards and analytical methods will be available for the increasingly interested
herb community, from policy-makers to entrepreneurs. It is also expected to con-
tribute to bringing economic benefit to African nations in the sustainable e xploitation
of their natural resources, and create an environment conductive to the growth of the
plant-based medicine industry.
2 Conclusion
MAPs have been accompanying mankind from the early times of civilizations. Our
forefathers, who had their origin in Africa, used natural substances of plant origin,
in order to ease their pains, heal their wounds, etc. These healers can be regarded as
forefathers of Traditional Medicine. A heritage of Africa, that is still servicing
approx. 80% of the population of this huge continent that is home to 55 countries,
800 languages and an unmeasurable wealth of plant diversity.
The continent with a varied flora of an estimated 68,000 plant species is home to
about 35,000 endemic (Cunningham 1993) species, growing in seven centers of
endemism.
Due to the nearly permanent renewed interest in the utilization of MAPs, Africa
and it’s not yet fully explored medicinal plant resources, frequently come to the
forefront of interest.
There is a tremendous potential for developing new crops and new products, in
Africa. In view of the recently significantly increased requirements for quality,
safety and efficacy of both botanicals and their products, there remains much to be
done to provide convincing proofs through clinical studies on the varied pharmaco-
logical activities of African MAPs (e.g.: antimalarial and other Antiprotozoal prod-
ucts, anti-proliferative potential, anti-inflammatory and analgesic activities,
anti-diabetes activity antioxidant activity and activity on the reproductive cardiovas-
cular and central nervous systems, etc.). These trials seem to offer a nearly unlim-
ited scope for exploration. This is witnessed by the fact that ca. 60% of all recent
publications on African medicinal plants, appeared during the last decade.
Despite a marked recent increase in the number of publications on indigenous
medicinal plants and dietary supplements, Africa is lagging behind Europe and Asia
in terms of the number of products that have been commercialized and the percent-
age of the flora that is utilized for international trade.
The growing demand on fewer resources, has resulted, in some cases, in the local
disappearance of favored and effective sources of botanicals and reduced species
diversity. By elaborating international and national standards of sustainability, these
dangers can be either eased or eradicated. Domestication of wild-crafted species can
also contribute to preserve the genetic resources and to reduce the sometimes
imminent threats of extinction. The recent initiative to develop national and ulti-
mately an African Herbal Pharmacopoeia is likely to mark an important milestone
in meeting quality, safety and efficacy standards both in African and international
perspectives.
Acknowledgements Thanks are due to Ms. J.V. van Hal, ProFound – Advisers In Development,
The Netherlands for her comments.
References
Amoa Onguéné P et al (2013) The potential of anti-malarial compounds derived from African
medicinal plants, Part I: A pharmacological evaluation of alkaloids and terpenoids. Malar
J 12:449
Anonymous (2005) Conservation international 2005 annual report, Washington, DC
Atawodi SE (2005) Antioxidant potential of African medicinal plants. J Biotechnol
4(February):128–133
Bakewell-Stone P, Lieblein G, Francis C (2008) Potentials for organic agriculture to sustain liveli-
hoods in Tanzania. Int J Agric Sustain 6(1):22–36
Barrett HR et al (2001) Smallholder farmers and organic certification: Accessing the EU market
from the developing world. Biol Agric Hortic 19(2):183–199
Blumenthal M (2011) African herbal pharmacopoeia – book review. HerbalGram 90:71–72
Brummitt RK, Pando F (2001) World geographical scheme for recording plant distributions, 2nd
edn. Hunt Institute for Botanical Documentation Carnegie Mellon University, Pittsburgh
Brummitt R et al (2001) World geographical scheme for recording plant distributions. International
Working Group on Taxonomic Databases for Plant Sciences (TDWG), Pittsburgh
Canter PH, Thomas H, Ernst E (2005) Bringing medicinal plants into cultivation: opportunities
and challenges for biotechnology. Trends Biotechnol 23(4):180–185
Cocks M, Dold A (2000) The role of ‘African chemists’ in the health care system of the Eastern
Cape province of South Africa. Soc Sci Med 51(10):1505–1515
Cunningham AB (1989) Herbal medicine trade: a hidden economy. Indic South Africa 6(3):51–54
Cunningham A (1993) African medicinal plants. Setting priorities at the interface between conser-
vation. UNESCO, Paris
Cunningham A (1997) An Africa-wide overview of medicinal plant harvesting, conservation and
health care. In: Bodeke G, KKS B, Burly J, Vantomme P (eds) Medicinal plants for forest con-
servation and health care. FAO, Rome, pp 116–129
Dzoyem JP, Tshikalange E (2013) Medicinal plant research in Africa. Elsevier, Oxford
Dzoyem JP, Tshikalange E, Kuete V (2013) Medicinal plants market and industry in Africa. In:
Medicinal plant research in Africa. Elsevier, Oxford, pp 859–890
Elujoba A, Odeleye O, Ogunyemi C (2005) Traditional medicine development for medicinal and
dental primary health care delivery system in Africa. African J Tradit Complemen Altern Med
2(1):46–61
FAO (2012) FAO statistical yearbook 2012 – Africa food and agriculture. FAO, Rome
Fennell CW et al (2004) Assessing African medicinal plants for efficacy and safety: Pharmacological
screening and toxicology. J Ethnopharmacol 94(2–3):205–217
Gurib-Fakim A, Kasilo M (2010) Promoting African medicinal plants through an African herbal
pharmacopoeia. Afr Health Moni Spec Issue Decade Afr Tradit Med 14:63–66
IndexMundi, 2016. Endangered plant species in Africa. (Retrieved 06 May 2017. at: http://www.
indexmundi.com/facts/indicators/EN.HPT.THRD.NO/map/africa)
Iwu M (2014) Handbook of African medicinal plants, 2nd edn. CRC Press, Boca Raton
Jimu L (2011) Threats and conservation strategies for the African cherry (Prunus africana) in its
natural range – a review. J Ecol Nat Environ 3(4):118–130
Juliani R, Simon JE, Ho CT (2007) African natural plant products: new discoveries and challenges
in chemistry and quality. American Chemical Society, Washington, DC
Klopper R, Smith GF, Van Rooy J (2002) The biodiversity of Africa. In: Baijnath H, Singh Y (eds)
Rebirth of science in Africa: a shared vision for life and environmental sciences. Umdaus Press,
Hatfield, pp 60–86
Kuete V (2013) Medicinal plant research in Africa pharmacology and chemistry. In: Kuete V (ed)
Elsevier insights. Elsevier, London/Waltham, pp 251–260
Kuete V, Viertel K, Efferth T (2013) Antiproliferative potential of African medicinal plants. In:
Medicinal plant research in Africa: pharmacology and chemistry. Elsevier, Oxford, pp 711–724
Makunga N, Philander L, Smith M (2008) Current perspectives on an emerging formal natural
products sector in South Africa. J Ethnopharmacol 119(3):365–375
Mander M, Le Breton G (2005) Southern African trade directory of indigenous natural products.
In: McKenzie MMM (ed) . Commercial Products from the Wild Group, Stellenbosch
Mander M, Steytler N, Diederichs N (2006) The economics of medicinal plant cultivation. In:
Diederichs N (ed) Commercializing medicinal plants. A Southern African guide. Sun Press,
Stellenbosch, p 215
Market Analysis and Research, I.T.C. (ITC) Trade map – list of exporters for the selected product
group (spices and herbs). Title – Trade map trade statistics for international business develop-
ment. Available at: http://www.trademap.org/cbi/Country_SelProduct_TS.aspx. Accessed 12
Feb 2016
Máthé Á (2015) Botanical aspects of medicinal and aromatic plants. In: Máthé Á (ed) Medicinal
and aromatic plants of the world. Springer, Dordrecht, pp 13–33
McGaw L, Ee E, Eloff J (2014) Toxicological survey of African medicinal plants. In: Kuete V
(ed) Toxicological survey of African medicinal plants. Elsevier, London/Waltham, pp 181–233
Myers N (1988) Threatened biotas: “hot spots” in tropical forests. Environmentalist 8(3):187–208
Myers N et al (2000) Biodiversity hotspots for conservation priorities. Nature 403(6772):853–858
Okigbo R, Mmeka E (2006) An appraisal of phytomedicine in Africa. KMITL Sci Technol
J 6(2):83–94
Okigbo R, Eme U, Ogbogu S (2008) Biodiversity and conservation of medicinal and aromatic
plants in Africa. Biotechnol Mol Biol Rev 3(6):127–134
Olson DM et al (2001) Terrestrial ecoregions of the world: a new map of life on earth. BioScience
51(11):933–938
Profound Advisers in Development (2014) Wild-collected botanicals and the EU market, BTC,
Belgian Development Agency
Rukangira E (2001) The African herbal industry: constraints and challenges|Elton Mukama –
Academia.edu. Erboristeria Domani. Available at: https://www.academia.edu/3443773/The_
African_herbal_industry_constraints_and_challenges. Accessed 13 Feb 2016
Simon J et al (2007) Issues in new crops and uses. In: Janick J, Whipkey A (eds) Issues in new
crops and uses. ASHS Press, Alexandria, pp 322–331
Stewart KM (2003) The African cherry (Prunus africana): can lessons be learned from an over-
exploited medicinal tree? J Ethnopharmacol 89(1):3–13
Tanzanian Organic Agriculture Movement (TOAM). (Retrieved: 06 May 2017. at: http://www.
kilimohai.org/?L=0)
Tekwu EM, Beng VP, Kuete V (2013) African medicinal plants acting on the reproductive, cardio-
vascular, and central nervous systems. In: Medicinal plant research in Africa: pharmacology
and chemistry. Elsevier, Oxford, pp 805–841
Udvardy M (1975) A classification of the biogeographical provinces of the world. IUCN, Morges
van Seters A (1997) Forest based medicines in traditional and cosmopolitan health care. In:
Bodeker G et al (eds) Medicinal plants for forest conservation and health care. FAO, Rome,
pp 5–11
Van Wyk BE (2011) The potential of South African plants in the development of new medicinal
products. S Afr J Bot 77(4):812–829
Van Wyk BE (2015) A review of commercially important African medicinal plants.
J Ethnopharmacol 176:118–134
Van Wyk BE et al (2009) Medicinal plants of South Africa, 2nd edn. Briza Publications, Pretoria
White F (1983) The vegetation of Africa: a descriptive memoir to accompany the UNESCO/
AETFAT/UNSO vegetation map of Africa. Nat Resour Res 20:1–356
Wiersum KF (1997) From natural forest to tree crops, co-domestication of forests and tree species,
an overview. Neth J Agric Sci 45:425–438
Wiersum K, Shackleton C (2005) African forests between nature and livelihood resource: inter-
disciplinary studies in conservation and forest management. In: Ros-Tonen M, Dietz T (eds)
African studies. Edwin Mellen Press, Lewiston, pp 67–91
Wiersum K et al (2006) Medicinal and aromatic plants. Agricultural, commercial, ecological,
legal, pharmacological and social aspects. In: Bogers R, Craker L, Lange D (eds) Frontis.
Springer, Dordrecht, pp 43–58
A Review of African Medicinal and Aromatic
Plants
Ben-Erik Van Wyk
Abstract The botanical and cultural diversity of the African continent provides
numerous opportunities for the development of innovative new crops and new prod-
ucts. However, many of African medicinal and aromatic plants remain scientifically
poorly known and in need of detailed investigation. To contribute to this process a
broad review of commercially important African medicinal and aromatic plants is
presented. The following data are given for a selection of 246 indigenous and 12
naturalised/commonly cultivated species: Scientific name, author citation, plant
family, trade name and/or vernacular name(s), part(s) used, main uses, region of
origin within Africa, African countries involved in local, regional or international
trade and recent references (general publications since 2000). Important publica-
tions at global, continental and regional levels are briefly mentioned. The categories
of use include traditional medicines, phytomedicines, dietary supplements and
functional foods, sources of extracts or chemical entities and sources of essential
oils. Examples of several African plants and their products in each of these catego-
ries are illustrated in colour.
Keywords Aromatic plants • Central Africa • Chemical entities • Dietary supple-

ments • East Africa • Essential oils • Mascarene Islands • Medicinal plants • North
Africa • Phytomedicines • Southern Africa • Traditional medicine • West Africa
1 Introduction
Africa is a continent of tremendous biological and cultural diversity that have

remained poorly explored from a scientific and commercial perspective. In recent
years however, there has been a surge of research interest in Africa as a source of
natural products. A recent review of African medicinal plants (Van Wyk 2015)
showed that 60% of the scientific papers on the most important plants appeared in
B.-E. Van Wyk (*)

Department of Botany and Plant Biotechnology, University of Johannesburg,
P.O. Box 524, Auckland Park, 2006 Johannesburg, South Africa

DOI 10.1007/978-94-024-1120-1_2
20 B.-E. Van Wyk
the last decade. African medicinal plants and plant-derived chemical entities repre-
sent about 10% of the total diversity in international markets. Coffea arabica and
Aloe vera are arguably the most valuable of all plant-based natural products in
global trade. At least 10 African plant species feature prominently in international
markets as functional foods and nutraceuticals (Van Wyk 2015). The African flora
undoubtedly represents a rich source of inspiration for cultural, scientific and com-
mercial innovations.
While the explicit aim of this volume is to demonstrate the wealth and diversity
of medicinal and aromatic plants of the African continent, this chapter is aimed at
providing the reader with a bird’s eye view of the most prominent and popular
African species.
Diversity of the African Flora
The total number of plant species in Africa has not yet been accurately recorded and
new species are continuously being described. However, a fairly accurate estimate
is possible. In their checklist for sub-Saharan Africa, Klopper et al. (2006) listed
50,136 species, with 32,424 taxa (including subspecies and varieties) in tropical
Africa and 22,755 taxa in southern Africa. The Cape Flora represents a special
hotspot of diversity and endemism, with 9000 species (Manning and Goldblatt
2012). The North African flora comprises an estimated 10,000 species (Dobignard
and Chatelain 2010–2013), while the figure for Madagascar is estimated at 11,350
species (Madagascar Catalogue 2013). The total number of African species is,
therefore, close to 71,500. In a recent publication from the Royal Botanic Gardens
Kew, it was estimated that there are 391,000 vascular plants known to science
(Royal Botanic Gardens Kew 2016). The African flora represents roughly 18% of
the total species diversity of the world.
Popular and Commercially Relevant African Plant Species
Based mainly on the literature discussed below, and the results of recent review
papers, an attempt was made to provide a summary of the most important and well-
known African medicinal and aromatic plants (Table 1). The list focuses strongly on
indigenous species (246 are listed) and includes only 12 very prominent exotic spe-
cies that are naturalized and/or widely cultivated. Naturalized species are commonly
used in traditional medicine in Africa and many non-indigenous species are often
included in publications (e.g. Vasisht and Kumar 2004 and Iwu 2014). However,
most of these are excluded here. The regional classification of African countries
follows Van Wyk (2015) and author citations for scientific names follow The Plant
List (2013). Examples of well-known species are illustrated in Figs. 1, 2, 3, 4, 5.
Table 1 Commercially relevant African medicinal and aromatic plants
African countries
Species, author citation and Origin involved in local,
family (non-indigenous Trade name and/or (see regional or References
species)* vernacular name Part(s) used Main uses (see caption) caption) international trade (see caption)
Abrus precatorius L. Crab’s eye vine, coral Leaves (seeds are TM (asthma, bronchitis, PA, Ma Many countries 1, 2, 3, 11
(Fabaceae) pea extremely toxic) coughs, stomach cramps,
malaria, physical and
sexual asthenia; wounds)
Acacia drepanolobium Whistling thorn Gum TM (root: diuretic; bark: EA Ethiopia (gum) 1, 3
Sjöstedt (Fabaceae) pharyngitis); gum as for
gum Arabic
Acacia karroo Hayne Sweet thorn Gum TM (bark: diarrhea; root: SA, EA South Africa, 1, 2, 3
(Fabaceae) colic); gum as for gum Zimbabwe
Arabic
Acacia nilotica (L.) Willd. Egyptian thorn Gum TM (all parts); gum as for EA Algeria, 1, 2, 5
ex Delile (Fabaceae) gum arabic Mozambique,
A Review of African Medicinal and Aromatic Plants
South Sudan,
Tanzania
Acacia senegal (L.) Willd. Gum arabic Gum (exudate) TM (bark, root); gum: WA, EA Central African 1, 2, 3, 8, 10,
(Fabaceae) emollient, wound healing); Republic, Chad, 11
pharmaceutical aid Eritrea, Ethiopia,
(emulsifier, stabilizer, Ghana,
additive) Madagascar, Mali,
Nigeria, Rwanda,
South Sudan,
Tanzania
Acacia seyal Delile Red acacia Gum TM (bark, root, leaf); gum EA Ethiopia, South 1, 3, 5
(Fabaceae) as for gum Arabic Sudan
(continued)
21
22
Table 1 (continued)
African countries
Acanthosicyos horridus !Nara Seeds TM (root); seed oil (edible, SA Namibia 1, 2
Welw. ex Hook.f. cosmetics)
(Cucurbitaceae)
Achillea fragrantissima Lavender cotton; Herb TM (general medicine) NA Egypt 3, 5
(Forssk.) Sch. Bip. yarrow
(Asteraceae)
Achyranthes aspera L. Chaff flower; devil’s Leaves TM (many ailments) PA Many countries 1, 2, 10
(Amaranthaceae) horsewhip
Acokanthera oppositifolia Bushman poison All parts TM (snake bite, headache, EA, SA Kenya, South 1, 2, 5, 6
(Lam.) Codd many uses) Africa
(Apocynaceae)
Adansonia digitata L. Baobab Dry fruit pulp TM (all parts); DS (fruit PA Algeria, Burkina 1, 3, 4, 8, 11
(Malvaceae) pulp: anti-oxidant) Faso, Madagascar,
Malawi, Nigeria,
South Africa,
South Sudan,
Tanzania,
Zimbabwe
Aerva javanica (Burm.f.) Desert cotton Leaves, roots TM (skin ailments) NA, EA Egypt, Kenia 1, 2, 3
Juss. (Amaranthaceae)
B.-E. Van Wyk
African countries
Aframomum melegueta Melegueta pepper Seeds TM (leaf, fruit, seed); DS WA, CA Cameroon, 1, 3, 11
K.Schum. (Zingiberaceae) (spice); ES Democratic
Republic of the
Congo (hereafter
D.R. Congo),
Equatorial Guinea,
Gabon, Ghana
Agathosma betulina (Berg.) Round leaf buchu Leaves TM (urinary antiseptic); SA South Africa 1, 2, 3, 7, 8,
Pillans (Rutaceae) DS (bitter tonic); ES (food 10, 11
flavourant)
Ageratum conyzoides L. Goat weed, billy-goat Leaves TM (wound healing, WA, CA, Burundi, 1, 2, 4
(Asteraceae)* weed diarrhea, dyspepsia, as EA D.R. Congo,
febrifuge; many uses) Ethiopia, Ghana,
Nigeria
Albizia adianthifolia Flat crown, Bark TM (diversity of ailments) PA Kenya, South 1, 2
(Schumach.) W.Wight umgadankawu Africa
(Fabaceae)
Alchornea cordifolia Christmas bush, Leaves, roots, TM (many uses: PA Burkina Faso, 1, 6
(Schumach. & Thonn.) dovewood fruit antimicrobial, Ghana
Muell.-Arg.; A. floribunda antispasmodic;
Muell.-Arg.; antidepressant)
(Euphorbiaceae)
Alepidea amatymbica Eckl. Ikhathazo Rhizome and TM (respiratory ailments – SA, EA South Africa, 1, 2, 3
& Zeyh., A. cordifolia B.-E. roots colds and influenza) Zimbabwe
van Wyk and other spp.
(Apiaceae)
Allanblackia floribunda Tallow tree Seeds TM (bark, fruit, leaves); CA, WA Cameroon, Côte 1, 3
Oliv. (Clusiaceae) seed oil (edible, cosmetics) d’Ivoire
Aloe arborescens Mill. Krantz aloe, kidachi Leaf DS (bitter tonic) SA Japan 1, 2, 6
(Asphodelaceae) aloe
(continued)
23
24
Table 1 (continued)
African countries
Aloe ferox Mill. and other Cape aloe Exudate, leaf gel TM (laxative); DS (gel SA South Africa 1, 2, 6, 8, 10,
species (Asphodelaceae) drinks, bitter tonic, 11
flavourant)
Aloe secundiflora Engl.; A. Kenya aloes Exudate TM (laxative) EA Kenya 1, 6
lateritia Engl.; A.
turkanensis Christian
(Asphodelaceae)
Aloe vera L. Aloe vera Leaf gel DS (tonic drinks); leaf gel Cu South Africa, 1, 6, 10, 11
(Asphodelaceae) (cosmetic, wounds) Nigeria
Ammi majus L. (Apiaceae) Bishop’s weed Fruits TM (many uses); CH EA South Sudan, 2, 5, 11
(xanthotoxin: psoriasis, Ethiopia, Somalia
leukoderma, vitiligo) (India)
Ammi visnaga – see Visnaga daucoides
Ammodaucus leucotrichus Cumin du Sahara Leaves, fruits TM (digestive system NA Morocco, Algeria 5
Coss & Durr. (Apiaceae) ailments)
Anabasis articulata Jointed anabasis Herb TM (skin diseases, NA 3
(Forssk.) Moq. diabetes); soap industry
(Amaranthaceae)
Anacyclus pyrethrum L. Spanish pellitory Root TM (diversity of uses; NA Morocco and 1, 5
(Asteraceae) kidney ailments; promotion Algeria
of salivation)
Anogeissus leiocarpa (DC.) African birch, All parts TM (anthelmintic, chewing WA Senegal to 1, 3
Guill. & Perr. ngalama sticks, veterinary medicine) Cameroon
(Combretaceae)
B.-E. Van Wyk
African countries
Antidesma Bois bigaignon Leaves, bark TM (dysentery, fever, Ma Madagascar 4, 8
madagascariensis Lam. diabetes)
(Euphorbiaceae)
Aphloia theiformis Benn. Fandamane Leaves, roots TM (leaves: malaria, fever; Ma Madagascar 1, 2, 4, 8
(Flacourtiaceae) root: worms)
Argania spinosa (L.) Skeels Argan (oil) Seeds TM: skin conditions, NA Morocco 5
(Sapindaceae) cosmetic; edible oil
Artemisia afra Jacq. ex African wormwood Herb TM (digestive and SA, EA Ethiopia, South 1, 2, 8, 10,
Willd. (Asteraceae) respiratory ailments); ES Africa, Tanzania, 11
(fragrance) Zimbabwe
Artemisia herba-alba Asso White wormwood Herb TM (antiseptic, NA Tunisia, Egypt 1, 5
(Asteraceae) antispasmodic, carminative,
vermifuge); ES
Artemisia judaica L. Judean wormwood Herb TM (gastrointestinal NA Egypt 5
(Asteraceae) ailments, anthelmintic); ES
Artemisia rehan Chiov. (=A. Ariti Herb Ritual incense; ES EA Ethiopia 4
absinthium L.?) (fragrance)
(Asteraceae)
Aspalathus linearis Rooibos tea Herb TM (antispasmodic); DS SA South Africa 1, 2, 3, 7, 8,
(Burm.f.) R. Dahlgren (health tea); cosmetics 10, 11
(Fabaceae)
Athrixia phylicoides DC. Bush tea Herb (tea) DS (health tea) SA South Africa 2
(Asteraceae)
Aucoumea klaineana Pierre Oukoumé Resin TM (general health) WA Gabon 1
(Burseraceae)
Azadirachta indica A.Juss.* Neem All parts TM (malaria and many Asia Chad, Kenya, 1, 3, 10, 11
(Meliaceae) other ailments); seed oil Madagascar, Niger,
(antifertility agent) South Sudan,
Tanzania
(continued)
25
26
Table 1 (continued)
African countries
Baillonella toxisperma African pearwood tree, Bark, seeds TM (bark); seed oil CA Cameroon 1, 3
Pierre (Sapotaceae) moabi tree (cosmetics)
Ballota africana L. African horehound Herb TM (colds, fever, cough, SA South Africa 1, 2, 10
(Lamiaceae) insomnia); ES
Ballota undulata (Sieber ex Horehound Herb TM (skin allergy); ES NA Egypt 3
Fresen.) Benth. (Lamiaceae)
Balanites aegyptiaca (L.) Desert date All parts, fruits, DS (fruits); seed oil (food, NA, CA, Algeria, Chad, 1, 5, 8
Delile (Zygophyllaceae) seeds cosmetics) EA Ethiopia,
Madagascar, South
Sudan
Bersama abyssinica Fresen. Winged bersama, Bark, leaves, TM (anthelmintic, WA, EA, Ethiopia and many 1, 6
(Melianthaceae) umukaka roots dysentery, roundworm, SA other countries
hemorrhoids, aphrodisiac)
Boophone disticha (L.f.) Poison bulb Bulb TM (analgesic, wound SA Lesotho, South 1, 2, 11
Herb. (Amaryllidaceae) healing; hallucinogen) Africa, Swaziland
Boswellia frereana Birdw. Maydi frankincense Resin TM (topical); traditional EA Somalia only 3
(Burseraceae) incense; ES (fragrance)
Boswellia neglecta S.Moore Olibanum Gum-resin TM (topical); traditional EA Ethiopia, Kenya 1
(Burseraceae) incense
Boswellia papyrifera (Del. Ethiopian frankincense Gum-resin TM (topical); traditional EA Ethiopia, Eritrea, 1
ex Caill.) Hochst. incense; ES (fragrance) South Sudan
(Burseraceae) ()
Boswellia rivae Engl. Frankincense Gum-resin TM (topical); traditional EA Ethiopia
(Burseraceae) incense; ES (fragrance)
B.-E. Van Wyk
African countries
Boswellia sacra Flueck. Arabian frankincense Gum-resin TM (topical); traditional EA Somalia 8, 10, 11
(=B. carteri Birdw.) incense; ES (fragrance)
(Burseraceae)
Bowiea volubilis Harv. ex Climbing potato, Bulb TM (headache, SA South Africa 1, 2
Hook.f. (Hyacinthaceae) igibisila constipation, oedema,
infertility, cystitis)
Bridelia atroviridis Fever leaf, mkarati Bark TM (purgative and diuretic) WA Nigeria 1, 6
Muell.-Arg.
(Phyllanthaceae)
Brucea antidysenterica Abalo, waginos Leaves, roots TM (diarrhea, indigestion, EA Ethiopia, Kenya 1, 6
J.F.Mill. (Simaroubaceae) skin problems, leprosy)
Bulbine frutescens (L.) Bulbine, burn jelly Leaf gel TM (topical); cosmetics SA South Africa 1, 2, 8, 11
Willd. (Asphodelaceae) plant
Cajanus cajan (L.) Millsp.* Pigeon pea Leaves, seeds, TM (leaves: sickle-cell India Kenya, Malawi, 1, 2, 8, 11
(Fabaceae) roots anaemia, numerous Nigeria, Uganda,
ailments); DS (seeds) Tanzania
Calotropis procera (Aiton) Giant milkweed, arka Roots, bark, TM (root: diarrhea, NA, WA, Egypt, Libya, 1, 5, 9
W.T. Aiton (Apocynaceae) (Ayurveda) leaves dysentery, dyspepsia; EA Benin, Ethiopia,
leaves or latex: ulcers, Ghana, Kenya,
leprosy) Somalia, Sierra
Leone, South
Sudan, Togo
Cananga odorata (Lam.) Ylang-ylang Fresh flower ES (fragrance) Asia Madagascar 1, 3, 4
Hook.f.* (Annonaceae)
Cannabis sativa L.* Marijuana Herb TM (analgesic, anti-emetic, Asia Ethiopia, South 1, 2, 6, 7, 10,
(Cannabaceae) asthma); intoxicant Africa and other 11
countries (usually
illegal; major trade
item)
(continued)
27
Table 1 (continued)
28
African countries
Capparis spinosa L. and Caper bush Herb, roots, fruits TM (herb: rheumatism; NA North African 3, 5
other species (Capparaceae) roots: diuretic, astringent, countries
tonic)
Caralluma adscendens Caralluma Stems TM (tonic and other uses); WA, EA, India 9
(Roxb.) R.Br. DS (putative appetite NA
(Apocynaceae) suppressant)
Carapa procera DC. African crabwood Seeds seed oil: cosmetic uses; CA, WA Cameroon, Congo, 1
(Meliaceae) TM (bark, leaves, seed oil: Gabon, Ivory
fever, respiratory and Coast, Mali,
digestive ailments) Senegal
Carissa edulis (Forssk.) Simple spined carissa Roots, leaves, TM (numerous ailments); PA Cameroon, 1, 2, 4, 8
Vahl. (Apocynaceae) fruits DS (fruits: appetizer for Ethiopia, Kenya,
invalids) Madagascar,
Namibia, South
Africa, Tanzania,
Zimbabwe
Catha edulis (Vahl.) Forssk. Khat Roots, bark, TM (roots, bark); DS (fresh SA, EA Ethiopia, Somalia 1, 2, 10, 11
(Celastraceae) leaves leaves: stimulant)
Catharanthus roseus (L.) Madagascar Herb TM (diabetes and many Ma Burundi, 1, 2, 4, 6, 8,
G.Don (Apocynaceae) periwinkle other uses); CH (vinca Madagascar, 10, 11
alkaloids – vinblastine, Rwanda and other
vincristine and others: countries
leukemia and Hodgkin’s
lymphoma)
Cedrelopsis grevei Baill. Katrafay Bark TM (fever, back pain) Ma Madagascar 1, 4
(Rutaceae)
B.-E. Van Wyk
African countries
Cedrus atlantica L. Atlantic ceder Wood ES (Atlantic ceder wood NA Morocco
(Pinaceae) oil)
Centella asiatica (L.) Urb. Indian pennywort Herb PH (topical: prevention of Co Madagascar, 1, 2, 4, 7, 8,
(Apiaceae) scar tissue formation); CH Tanzania 10, 11
(asiaticoside: vulnerary)
Ceratonia siliqua L. Carob tree Fruit pulp, seeds TM (diarrhea, vomiting); NA Arabia, 2, 5, 11
(Fabaceae) seed gum: thickener, Mediterranean
dietary supplement countries; widely
cultivated
elsewhere
Chenopodium ambrosioides Wormseed Seeds TM (anthelmintic, fever); SAm, Many countries 1, 5, 10, 11
L.* (Amaranthaceae) ES (anthelmintic) CAm (Egypt, Burundi,
Rwanda, South
Africa, Zimbabwe)
Chironia baccifera L. Christmas berry Herb TM (hemorrhoids, ulcers, SA South Africa 1, 2
(Gentianaceae) acne)
Cinchona ledgeriana Quinine tree Bark TM (malaria, appetite SAm Burundi, 1, 10, 11
(How.) Bern.Moens. and stimulant) D.R. Congo,

other spp.* (Rubiaceae) Guinea, Kenya,
Madagascar,
Rwanda, Tanzania
Cissampelos pareira L., C. Velvetleaf All parts TM (gout, syphilis and PA Almost all 1, 2, 3, 4, 6
mucronata A.Rich. numerous other ailments); countries
(Menispermaceae) CH (cissampeline: skeletal
muscle relaxant)
Citrullus colocinthus (L.) Colocynth Fruit TM (purgative) NA Egypt and all other 1, 5, 11
Schrad. (Cucurbitaceae) North African
countries
Citrullus lanatus (Thunb.) Kalahari melon Seeds Seed oil (edible, SA Botswana 1, 2
Matsum. & Nakai cosmetics); also TM
(Cucurbitaceae)
29
(continued)
30
Table 1 (continued)
African countries
Cladanthus mixtus (L.) Moroccan chamomile Herb TM (topical); ES NA Morocco, Algeria, 5
Chevall. (Asteraceae) (cosmetics) Tunisia, Libya
Clausena anisata (Willd.) Horsewood Root, leaves TM (numerous ailments; PA Many countries 1, 2, 4, 9
Hook.f. (Rutaceae) chewing sticks)
Cleome chrysantha Decne Spider flower Herb TM (anthelmintic, infantile NA Egypt 3
(Brassicaceae) convulsions)
Cleome droserifolia Spider flower Herb TM (diabetes and NA, EA Egypt, Libya, 3, 5
(Forssk.) Delile numerous other ailments) Ethiopia
(Brassicaceae)
Clivia miniata (Lindl.) Bush lily Whole plant TM (uterotonic) SA South Africa 1, 2
Regel (Amaryllidaceae)
Coffea arabica L. Coffee Seeds CH (caffeine: CNS EA Ethiopia, Kenya, 1, 3, 10, 11
(Rubiaceae) stimulant) South Africa,
Tanzania
Cola acuminata (P.Beauv.) Cola nut tree, abata Seeds TM (masticatory, tonic, WA, CA Cameroon; also 1, 3, 7, 10,
Schott. & Endl. kola many uses); CH (caffeine, D.R. Congo, 11
(Malvaceae) theobromine: stimulant) Gabon, Ghana,
Northern Nigeria,
Senegal, Sudan
Cola nitida (Vent.) Schott. Cola nut tree, gbanja Seeds TM (masticatory, tonic, WA, CA Ghana, Sierra 1
& Endl., (Malvaceae) kola many uses); CH (caffeine, Leone, Northern
theobromine: stimulant) Nigeria, Burkina
Faso, Côte
d’Ivoire, Sudan
B.-E. Van Wyk
African countries
Colophospermum mopane Mopane Seeds ES (fragrance); TM (bark: SA, EA Namibia 1, 2
(J.Kirk ex Benth.) J. diarrhoea; many uses)
Léonard (Fabaceae)
Combretum coccineum Flame vine Seeds TM (seeds, “voan seeds”, Ma Madagascar 1, 4, 9
(Sonn.) Lam. voanta menaka:
(Combretaceae) anthelmintic)
Combretum micranthum Kinkeliba Leaves DS (tonic tea); also TM WA Côte d’Ivoire, 1, 4, 8, 9
G.Don (Combretaceae) (bark, leaves: cholagogue, Mali, Senegal
antipyretic, tonic; many
uses)
Combretum nigricans Lepr. Gum combretum Gum TM (root, leaves); edible WA Burkina Faso, 1, 3
ex Guill. & Perr. gum, similar to gum Arabic Nigeria
(Combretaceae)
Commiphora africana African myrrh, Gum-resin Incense; TM (all parts: PA Algeria, Chad 1, 2
(A.Rich) Engl. bdellium numerous ailments)
(Burseraceae)
Commiphora erythraea Opopanax tree Gum-resin Incense, TM, ES EA Somalia 7
Engl. (Burseraceae) (fragrance)

Commiphora guidottii Scented myrrh, bisabol Gum-resin Incense, TM, ES EA Ethiopia, Somalia 1
Chiov. (Burseraceae) (fragrance)
Commiphora myrrha (Nees) Myrrh tree Gum-resin TM (antiseptic); incense; EA Ethiopia, Somalia 1, 3, 8, 10,
Engl. (Burseraceae) ES (fragrance) 11
Commiphora wildii Merxm. Namibian myrrh tree, Gum resin ES (fragrance) SA Namibia
(Burseraceae) omumbiri
Cryptolepis sanguinolenta Ghana quinine Roots TM (malaria) WA, CA Ghana, 1, 8, 9
(Lindl.) Schltr. Madagascar, Mali
(Apocynaceae)
Cullen plicatum (Delile) Scurfy pea Herb TM (antibiotic, antiemetic) NA Egypt 3
C.H.Stirt. (Fabaceae)
(continued)
31
32
Table 1 (continued)
African countries
Curtisia dentata (Burm.f.) Assegai, umlahleni Bark TM (tonic, aphrodisiac, SA South Africa 1
C.A.Sm. (Curtisiaceae) diarrhea)
Cyclopia genistoides (L.) Honeybush Herb TM (health tea); CH SA South Africa 2, 8, 11
R.Br. and other spp. (mangiferin: antioxidant)
(Fabaceae)
Cymbopogon schoenanthus Camel grass Herb TM (antispasmodic); ES: NA North African 1, 3, 5
(L.) Spreng. (Poaceae) commercial skin care countries
products
Cyperus rotundus L. Nutsedge Tubers TM (numerous ailments); NA North African 1, 2, 5
(Cyperaceae) ES countries
Dacryodes edulis (G.Don) Safou, safu, butterfruit Seed DS (seed oil); also TM (all CA Cameroon, Gabon 1
H.J.Lam (Burseraceae) parts: many ailments)
Danais fragrans C.F.Gaertn. Liane jaune Roots, bark TM (analgesic, skin Ma Madagascar 1, 4, 8
(Rubiaceae) infections)
Detarium microcarpum Sweet dattock Root, bark, TM (diuretic, astringent; WA, CA Mali, Senegal and 1, 6
Guill. & Perr. (Fabaceae) leaves many uses) other countries
Dichrostachys cinerea (L.) Sickle bush Roots TM (numerous ailments) SA, EA, Many countries 1, 2, 6, 9
Wight & Arn. (Fabaceae) WA (South Africa,
Zambia,
Mozambique,
Zimbabwe)
Dicoma anomala Sond. Hloenya Root (leaves) TM (numerous ailments) SA, EA Lesotho, South 1, 2
(Asteraceae) Africa, Zimbabwe
B.-E. Van Wyk
African countries
Dicoma capensis Less. Koorsbossie Leaves TM (fever, diuretic) SA South Africa 1, 2
(Asteraceae)
Dodonaea viscosa Jacq. Sand olive, umusasa Leaves, roots TM (many ailments) EA, CA, Many countries 1, 2, 4
(Sapindaceae) SA
Drimia maritima (L.) Stearn Squill, sea squill Bulb TM (heart tonic, diuretic, NA All North African 5, 10, 11
(Asparagaceae) expectorant) countries
Drosera madagascariensis Sundew Herb TM (respiratory ailments) Ma Madagascar 2, 4
DC. (Droseraceae)
Echinops kebericho Mesfin Kebericho Roots TM (diarrhoea, stomach EA Ethiopia
(Asteraceae) ache, fever, typhus)
Embelia schimperi Vatke Inkoko Seeds TM (anthelmintic) EA Ethiopia, Tanzania 1
(Primulaceae)
Eucomis autumnalis (Mill.) Pineapple flower, Bulb TM (bone fractures, SA South Africa 1
Chitt. (Asparagaceae) amathunga numerous ailments)
Euphorbia hirta L. Asthma herb Leaves TM (amebic dysentery and Co Madagascar, 1, 2, 4, 6, 8
[=Chamaesyce hirta (L.) numerous other ailments) Mauritius and
Millsp.] (Euphorbiaceae) many other African

countries
Euphorbia peplus L. Petty spurge Latex CH (ingenol mebutate; skin NA, Asia, cultivated in 2, 6, 11
(Euphorbiaceae) ailments, solar keratosis) Europe Australia
Fadogia agrestis Schweinf. Baraboro Bark TM (aphrodisiac, erectile WA Nigeria 1
ex Hiern (= Vangueria dysfunction)
agrestis) (Rubiaceae)
Faidherbia albida (Delile) Ana tree Bark TM (toothache, cough, NA, WA, Algeria, Mali, 1, 2
A. Chev. (Fabaceae) diarrhea and other EA, SA South Sudan,
ailments) Ethiopia
Ferula communis L. Giant fennel Root, TM (many topical uses of NA, EA Morocco and other 1, 5
(Apiaceae) inflorescence the resin gum – called North African
fessoukh) countries
(continued)
33
34
Table 1 (continued)
African countries
Garcinia afzelii Engl., G. Garcinia Stems, roots Oral hygiene (toothbrush WA, CA Cameroon, Côte 1
mannii Oliv. and other spp. sticks) d’Ivoire, Equatorial
(Clusiaceae) Guinea, Gabon,
Ghana, Nigeria
Garcinia kola Heckel Bitter kola Seeds TM (numerous ailments); WA, CA Cameroon, 1, 8
(Clusiaceae) DS (antioxidant, D.R. Congo,
aphrodisiac, Equatorial Guinea,
immunostimulant) Nigeria, Sierra
Leone
Glinus lotoides Loefl. Damascisa, lotus Fruits TM (tapeworm, diarrhea) NA, EA Ethiopia, Egypt 1
(Molluginaceae) sweetjuice, metera
Gloriosa superba L. Flame lily Tuber, seeds TM (tuber; many uses); CH PA Cameroon, 1, 2, 6, 11
(Colchicaceae) (seeds; colchicine) Nigeria, Zimbabwe
Gnetum africanum Welw. Eru Leaves DS (leaves); TM CA Cameroon, 1
(Gnetaceae) (numerous uses) D.R. Congo,
Gabon
Griffonia simplicifolia (Vahl Griffonia Seeds PH/CH WA Côte d’Ivoire, 1, 6, 8, 11
ex DC.) Baill. (Fabaceae) (5-hydroxytryptophan: Ghana
neurological and
psychiatric disorders)
Guiera senegalensis Guiera Leaves TM (many uses; cough WA, NA Senegal and other 1, 9
J.F.Gmel. (Combretaceae) syrup) countries
Gunnera perpensa L. River pumpkin, Rhizome TM (impotence, infertility SA South Africa 1
(Gunneraceae) ugobho and many other uses)
B.-E. Van Wyk
African countries
Gymnema sylvestre (Retz.) Miracle fruit Leaves DS (diabetes, weight loss); PA India, USA 1, 9
R.Br.ex Schult. CH: gymnemic acid
(Apocynaceae) (antisweet activity)
Hagenia abyssinica (Bruce) Kosso Female flowers TM (anthelmintic) EA Ethiopia 1
J.F.Gmel. (Rosaceae)
Harpagophytum Devil’s claw Secondary roots TM (anti-inflammatory; SA Botswana, 1, 2, 3, 7, 8,
procumbens DC. ex Meisn., bitter tonic); PH (low back Namibia, South 10, 11
H. zeyheri Decne pain) Africa
(Pedaliaceae)
Harrisonia abyssinica Oliv. Baingou Root TM (many uses) PA Kenya 1, 6
(Simaroubaceae)
Harungana Haronga Bark, leaves TM (digestive ailments, PA Madagascar 1, 4, 8, 10,
madagascariensis Lam. ex topical uses) 11
Poir. (Hypericaceae)
Helichrysum odoratissimum Imphepho Leaves TM; ritual incense SA South Africa 1, 2
(L.) Sweet (Asteraceae)
Heteromorpha arborescens Parsley tree, mkatlala Stems TM (aphrodisiac, SA, EA Lesotho, South 1, 2
(Spreng.) Cham. & Schltdl. hypertension, headache) Africa, Zimbabwe
(Apiaceae)
Hibiscus sabdariffa L. Hibiscus, red sorrel Calyx and DS (antioxidant); TM CA Mali, Senegal, 1, 2, 3, 7, 8,
(Malvaceae) epicalyx (numerous uses; also South Sudan 10, 11
topical)
Hoodia gordonii (Masson) Hoodia, ghaap, ghôba Stem DS (functional food, to SA Botswana, 8, 9, 11
Sweet ex Decne suppress hunger and thirst) Namibia, South
(Apocynaceae) Africa
Hyoscyamus muticus L. Egyptian henbane Stems and leaves CH (tropane alkaloids – NA Egypt 3, 5, 6
(Solanaceae) hyoscyamine and
scopolamine); bronchial
diseases, ophthalmology
(continued)
35
36
Table 1 (continued)
African countries
Hypoxis hemerocallidea Hypoxis, African Corm DS (general tonic; prostate EA, SA Mozambique, 1, 2, 8, 10,
Fisch. & C.A.Mey. potato hyperplasia) South Africa 11
(Hypoxidaceae)
Ipomoea pes-caprae L. Goat’s foot Leaves, roots TM (antihistaminic, Co All coastal 1, 2, 4, 8, 9
(Convolvulaceae) antimicrobial, countries
anti-inflammatory)
Irvingia gabonensis Baill. Dika nut tree Seed oil DS (edible nut, oil); also CA, WA Cameroon, 1
(Irvingiaceae) TM (bark, leaves: Equatorial Guinea,
numerous ailments, weight Gabon
loss)
Jateorhiza palmata (Lam.) Calumba Root (radix TM (diarrhoea, dysentery EA Malawi, 1,6
Miers (Menispermaceae) calumbae) and other uses); DS (bitter Mozambique
tonic, anorexia nervosa)
Jatropha curcas L.* Purging nut Seeds, leaves TM (purgative; numerous SAm Many countries 1, 2, 4, 6, 11
(Euphorbiaceae) other uses)
Juniperus oxycedrus L. Prickly cedar, cade Wood, fruits, oil ES (cade oil, obtained from NA Morocco, Algeria, 5
(Cupressaceae) the wood): topical Tunisia
antiseptic, psoriasis,
chronic eczema
Juniperus phoenicia L. Phoenician juniper Leaves, fruits TM (leaves: digestive and NA Algeria, Morocco, 5
(Cupressaceae) urinary ailments); ES: Tunisia
antiseptic
Juniperus procera Hochst. African cedar Twigs and leaves TM (antifertility, EA Kenya 1
ex Endl. (Cupressaceae) expectorant, rheumatism);
ES (fragrance, medicinal)
B.-E. Van Wyk
African countries
Kigelia africana (Lam.) Sausage tree Fruit (bark, roots) TM (antimicrobial, PA Ethiopia, South 1, 2, 8, 10,
Benth. (Bignoniaceae) antineoplastic; numerous Africa and many 11
traditional uses) other countries
Lavandula antineae Maire Desert lavender Herb ES NA Algeria and Libya 5
(Lamiaceae)
Lavandula multifida L. Fernleaf lavender Herb TM (numerous ailments); NA Tunisia, Egypt 5
(Lamiaceae) ES
Lavandula stoechas L. Spanish lavender Herb TM (catarrh, colds, NA Morocco and other 5
(Lamiaceae) abdominal pain); ES countries
(aromatherapy)
Laurus nobilis L. Laurel, sweet bay Leaves, fruits Culinary herb; ES (topical NA Tunisia and other 5
(Lauraceae) use, flavourant) countries (widely
cultivated)
Leonotis leonurus (L.) R.Br. Wild dagga Herb TM (respiratory ailments, SA South Africa 1, 2
(Lamiaceae) numerous other uses)
Lepidium sativum L. Garden cress Seeds DS (colic, dysentery, EA South Sudan, 1, 11
(Brassicaceae) headache, asthenia, Ethiopia

measles, rheumatism)
Lessertia frutescens (L.) Sutherlandia, cancer Leaves DS (bitter tonic; diabetes, SA Lesotho, Namibia, 1, 2, 8, 10,
P.Boldblatt & J.C.Manning bush numerous ailments) South Africa, 11
[=Sutherlandia frutescens
(L.) R.Br.] (Fabaceae)
Lippia javanica (Burm.f.) Fever tea Herb TM (coughs, colds, SA, EA Mozambique, 1, 2
Spreng. (Verbenaceae) headache, numerous other South Africa,
ailments); ES (mosquito Zimbabwe
repellent)
Lippia scaberrima Sond. Mosukujane Herb DS (stomachic, tonic, SA Botswana 1, 2
(Verbenaceae) health tea)
Lophira lanceolata Van Dwarf red ironwood All parts TM (diversity of ailments) WA Guinea 1, 3
Tiegh. (Ochnaceae)
37
(continued)
38
Table 1 (continued)
African countries
Malva sylvestris L. Common mallow Leafy stems, TM (antitussive, topical NA Tunisia and other 2, 5, 7, 11
(Malvaceae) flowers antipruritic); flowers: countries
natural colourant
Massularia acuminata Massularia Bark, roots TM (chewing sticks, CA, WA Cameroon, Sierra 1
(G.Don) Bullock ex Hoyle aphrodisiac) Leone
(Rubiaceae)
Mentha longifolia (L.) Horse mint Herb TM (antispasmodic); ES NA, SA Egypt, South 1, 2, 3
Huds. (Lamiaceae) Africa
Merwilla plumbea (Lindl.) Inguduza Bulb TM (fertility, purgative, SA South Africa 1, 2
Speta (=Scilla natalensis wound healing)
Planch.) (Hyacinthaceae)
Momordica charantia L. Bitter melon Leaves TM (numerous ailments) PA Many countries 1, 2, 4, 7, 10
(Cucurbitaceae)
Mondia whitei (Hook.f.) White’s ginger, Root TM (aphrodisiac; numerous PA Benin, Kenya, 1, 8, 9, 11
Skeels (Apocynaceae) mondia, tonic root ailments; spice) South Africa,
Uganda,
Zimbabwe
Monodora myristica Calabash nutmeg, Fruit, seeds Fruit: essential oil, spice; PA Benin, Cameroon, 1
(Gaertn.) Dunal African nutmeg ES (flavourant); TM Equatorial Guinea,
(Annonaceae) (stimulant, stomachic, Gabon
many ailments)
Morinda lucida Benth. Brimstone tree Leaves, roots, TM (fever, diabetes and CA, WA Madagascar, 1
(Rubiaceae) bark numerous other ailments) Nigeria,
D.R. Congo
B.-E. Van Wyk
African countries
Moringa oleifera Lam.* Drumstick tree, all parts DS (health food); TM India Madagascar, South 1, 4, 8, 11
(Moringaceae) horseradish tree (many and diverse Sudan and most
ailments) tropical parts
Moringa peregrina Wild drumstick tree Leaves, seeds Seed oil: edible, cosmetic NA Egypt, Sudan 5
(Forssk.) Fiori uses
(Moringaceae)
Moringa stenopetala (Baker Ethiopian horseradish Leaves DS (health food) EA Ethiopia, Kenya
f.) Cufod. (Moringaceae) tree, African moringa
Myrianthus arboreus Giant yellow mulberry, Leaves, bark, DS (fruits, leaves); TM WA, EA Cameroon, Congo, 1
P. Beauv. (Urticaceae) bush pineapple roots (headache, pains, numerous Ghana, Ivory
ailments) Coast, Nigeria,
Sierra Leone,
Tanzania
Myrothamnus flabellifolius Resurrection plant Herb TM (respiratory ailments); SA Angola, 1, 2
(Sond.) Welw. ES (antimicrobial) Mozambique,
(Myrothamnaceae) Namibia, South
Africa, Zimbabwe
Myrtus communis L. Myrtle Leaves, fruits TM (expectorant, NA Egypt, Tunisia 5

(Myrtaceae) astringent, antiseptic); ES
(oil of myrtle)
Nauclea – see Sarcocephalus
Neobeguea mahafalensis Andy Bark TM (rheumatism, asthenia, Ma Madagascar 1
J.-F.Leroy (Meliaceae) erectile dysfunction)
Nerium oleander L. Oleander Root, leaves TM (topical applications); NA Morocco, Algeria, 1, 2, 5, 10,
(Apocynaceae) cardiotonic (toxic!) Tunisia, Libya 11
Ocimum gratissimum L. African basil, romba Herb TM, ES (colds, respiratory PA Burundi, 1, 2, 4
(Lamiaceae) ailments, headache, fever Madagascar,
and diversity of other uses) Nigeria
(continued)
39
40
Table 1 (continued)
African countries
Ocotea bullata (Burch.) Stinkwood, unukani Bark TM (headache, stomach SA South Africa 1, 2
Baill. (Lauraceae) complaints, nervous
disorders, infantile
diarrhoea)
Okoubaka aubrevillea Okoubaka Bark TM (many uses); DS WA, CA Ghana, Nigeria, 1, 6
Pellegr. & Normand (stomachic and tonic) Cameroon,
(Santalaceae) D.R. Congo
Olea europaea L. subsp. Wild olive, olive Leaves, bark, TM (urinary tract, colic, EA, CA, Many countries 1, 2, 4, 7, 10,
africana (Mill.) P.S.Greene; roots ophthalmia, tapeworm); SA 11
subsp. europaea (Oleaceae) TM (olive leaf, olive oil)
Origanum compactum Moroccan wild Herb TM; ES NA Morocco
Benth. (Lamiaceae) oregano
Origanum syriacum L. Za’ater, zatar, bible Herb TM (many uses), ES NA Egypt 5
(Lamiaceae) hyssop
Parinari excelsa Sabine Guinea plum Roots, bark TM (stomach complaints, WA, CA Central African 1
(Chrysobalanaceae) headache, wounds) Republic, Ghana,
Ivory Coast, Mali,
Senegal,
Parkia biglobosa (Jacq.) R. African locust bean Seeds (all parts) TM (wide diversity of uses) WA Chad, Nigeria 1
Br. ex Don (Fabaceae)
Parquetina nigrescens African parquetina, Leaves TM (anaemia and many PA Nigeria 1, 9
(Afzel.) Bullock ogbo other uses)
(Apocynaceae)
B.-E. Van Wyk
African countries
Pauridiantha paucinervis Tamirova Roots, leaves TM (headache, purgative, Ma Madagascar 1
(Hiern) Bremek. wounds)
(Rubiaceae)
Pausinystalia johimbe Yohimbe tree Bark CH: yohimbine WA, CA Cameroon 1, 3, 10, 11
(K.Schum.) Pierre (aphrodisiac)
(Rubiaceae)
Peganum harmala L. Harmal, African rue, Roots, leaves, TM (high blood pressure, NA Egypt, Morocco, 2, 5, 10, 11
(Zygophyllaceae) wild rue seeds haemorrhoids, bronchitis, Tunisia and other
rheumatism); CH:beta- countries
carboline alkaloids)
Pelargonium cv. Rosé (“P. Rose geranium Leaves ES (fragrance) SA Egypt, Tunisia, 2, 5
graveolens” = P. capitatum Kenya, Reunion,
(L.) L’Hérit. x P. radens South Africa
H. Moore) (Geraniaceae)
Pelargonium sidoides DC. Rooirabas, Roots PH (bronchitis, immune SA Lesotho, South 1, 2, 3, 7, 8,
(Geraniaceae) umckaloabo stimulant); TM (dysentery; Africa 10, 11
general tonic)
Pentadiplandra brazzeana L’oubli Roots, fruits TM (roots: pre- and WA, CA Cameroon, 1, 6
Baill. (Pentadiplandraceae) postnatal care); CH D.R. Congo,
(brazzein: protein Nigeria
sweetener)
Pentanisia prunelloides Icimamlilo Roots (leaves) TM (headache, pain, SA South Africa 1,2
(Klotzsch ex Eckl. & Zeyh.) purgative, wounds)
Walp. (Rubiaceae)
Physostigma venenosum Calabar bean Seeds CH (physostigmine: WA Cameroon 1, 10
Balf. (Fabaceae) cholinesterase inhibitor;
Alzheimer’s)
Phytolacca dodecandra Endod All parts TM (numerous ailments; EA Burundi, Ethiopia, 1, 2, 6
L’Hérit. (Phytolaccaceae) control of snails and Madagascar
bilharzia)
(continued)
41
42
Table 1 (continued)
African countries
Piper guineense Schumach. West African pepper Fruit; roots, Spice (fruit, essential oil); WA, CA Cameroon, Central 1
& Thonn. (Piperaceae) leaves ES (flavourant); TM African Republic,
(numerous ailments) Congo,
D.R. Congo,
Equatorial Guinea
Pistacia lentiscus L. Mastic tree, lentisc Gum resin (roots, TM (stomach ulcers); gum NA Tunisia, Morocco 1, 5
(Anacardiaceae) leaves, fruits) resin (mastic): dietary and (commercial
cosmetic preparations production mostly
on Chios, Greece)
Plantago afra L. Fleawort Ripe seeds TM (chronic constipation, NA Egypt 5, 10, 11
(Plantaginaceae) demulcent)
Prosopis africana (Guill. & African mesquite Seeds DS (fermented seeds); TM WA Mali, Nigeria 1
Perr.) Taub. (Fabaceae) (bark, roots, leaves:
numerous uses)
Prunus africana (Hook.f.) Red stinkwood, Bark PH (benign prostate PA Cameroon, 1, 2, 3, 4, 8,
Kalkman (Rosaceae) pygeum hyperplasia); TM (pain, D.R. Congo, 10, 11
fever, inflammation and Equatorial Guinea
other uses) (& Bioko),
Ethiopia, Kenya,
Madagascar,
Tanzania, Uganda
Rauvolfia media Pichon Madagascar Bark, roots CH (indole Ma Madagascar 6
(Apocynaceae) serpent-wood alkaloids – reserpiline)
B.-E. Van Wyk
African countries
Rauvolfia vomitoria Afzel. African serpent-wood Roots, bark TM (mild essential CA, WA, Cameroon, Central 1, 6, 8
(Apocynaceae) hypertension); CH: EA African Republic,
isolation of ajmaline Guinea, Kenia,
Madagascar, South
Sudan
Ravenala madagascariensis Traveller’s palm Leaves TM (antiseptic, fever) Ma Madagascar 1, 4, 8
Sonn. (Strelitziaceae)
Rhamnus prinoides L’Hérit. Geisho, umunanira Roots, bark, TM (colic, fever, EA Ethiopia, Rwanda 1, 2
(Rhamnaceae) leaves pneumonia, headache and
other uses)
Ricinodendron heudelotii Erimado, ground nut Seeds DS (seeds: nuts); TM WA, CA, Cameroon, 1
(Baill.) Pierre tree, musodo (roots, bark, leaves: many EA Equatorial Guinea
(Euphorbiaceae) uses)
Ricinus communis L. Castor oil plant Cold-pressed TM (seed oil: purgative; PA Burundi, Ethiopia, 1, 2, 5, 10,
(Euphorbiaceae) seed oil leaves, roots: many other Mozambique, 11
uses) [biodiesel] Nigeria, Rwanda,
South Africa
Rosa canina L. (Rosaceae) Wild rose, dog rose Flower buds, TM (numerous ailments; NA Algeria, Tunisia, 5, 7, 10, 11
fruits (rosehips) tonic); Morocco
Rosa gallica L. (Rosaceae)* Common rose Flower buds, TM (numerous ailments; NA Egypt, Algeria, 5
petals tonic); ES, source of rose Tunisia, Morocco
water
Rosmarinus officinalis L. Rosemary Herb TM (antispasmodic, NA Morocco, Algeria, 5, 7, 10, 11
(Lamiaceae) antiseptic, anti- Tunisia, Libya
inflammatory); ES (also widely
cultivated)
Ruta chalepensis L. Aleppo rue, Syrian rue Herb, roots TM (respiratory ailments, NA Morocco, Algeria, 1, 5, 9
(Rutaceae) rheumatism and many Tunisia, Libya
other uses); ES (also widely
cultivated)
(continued)
43
44
Table 1 (continued)
African countries
Salvadora persica L. Mustard tree, Roots, stems TM (toothbrush sticks, oral PA Algeria, Chad, 1, 2, 3, 10,
(Salvadoraceae) toothbrush tree hygiene) Egypt, Ethiopia, 11
Tanzania
Sarcocephalus latifolius African peach, African Roots, bark and TM (fever, malaria and WA, CA Benin, Burkina 1, 8
(Sm.) Bruce (= Nauclea quinine, opepe leaves many other uses) Faso, Cameroon,
latifolia Sm.) (Rubiaceae) D.R. Congo,
Gabon, Gambia,
Ghana, Ivory
Coast, Mali,
Nigeria, Sierra
Leone
Sceletium tortuosum (L.) Sceletium, kanna Whole plant TM/PH (hypnotic, sedative; SA South Africa 2, 8, 10, 11
N.E.Br. (= neurological and
Mesembryanthemum psychiatric disorders)
tortuosum L.) (Aizoaceae)
Sclerocarya birrea (A.Rich) Marula, umganu Seeds; bark, Seed oil (edible, cosmetic); SA, EA, Algeria, Botswana, 1, 2
Hochst. (Anacardiaceae) fruits TM (bark: diarrhoea, NA Mozambique,
dysentery, malaria and Namibia, South
many other uses) Africa, Swaziland,
Zimbabwe,
Tanzania
Sclerochiton ilicifolius Molomo monate Root bark CH (monatin/arruva: SA South Africa
A.Meeuse (Acanthaceae) sweetener, now
synthesized)
B.-E. Van Wyk
African countries
Securidaca Violet tree Root bark TM (a wide diversity of PA Ethiopia, 1, 2, 10, 11
longepedunculata Fresen. (leaves, stem ailments in most parts of Madagascar,
(Polygalaceae) bark) Africa) Malawi, Mali,
Mozambique,
Tanzania,
Zimbabwe
Senna alata (L.) Roxb.* Ringworm cassia Leaf TM (purgative, antifungal) Trop. Am. Benin, Congo, 1, 6
(Fabaceae) Ghana, Ivory
Coast, Nigeria,
Sierra Leone,
Uganda
Senna alexandrina Mill. Senna, Alexandria Leaf, fruit TM (stimulant laxative) NA, EA Sudan, Egypt, 1, 5, 6, 10,
(Fabaceae) senna Libya, Algeria, 11
South Sudan,
Ethiopia, Somalia,
Kenya
Senna occidentalis (L.) Coffeeweed, Roots, leaves TM (many ailments) PA Madagascar and 1, 2
Link (Fabaceae) tsotsorinangatra many other

countries
Senna italica Mill. Senegal senna Leaves, fruits TM (purgative); hair care PA Many countries; 1, 2, 6
(Fabaceae) Egypt and Sudan
(hair conditioner)
Sesamum indicum L. (= S. Sesame Seeds, seed oil DS (functional food: EA Ethiopia, South 1, 2, 3, 11
orientale L.) (Pedaliaceae) reduction of cholesterol Sudan,
and blood sugar levels)
Siphonochilus aethiopicus African ginger Rhizome and TM (anti-inflammatory, PA South Africa, 1, 2, 8, 10
(Schweinf.) B.L.Burtt roots anti-malarial); ES Mozambique,
(Zingiberaceae) (flavourant) Swaziland
(continued)
45
46
Table 1 (continued)
African countries
Solanum incanum L. Bitter apple Fruit, leaves, TM (wounds, pain, EA, CA, Botswana, 1, 2, 6
(Solanaceae) roots toothache, venereal SA Djibouti, Ethiopia,
diseases and numerous Namibia, Rwanda,
other ailments) South Africa,
Tanzania and other
countries
Solenostemma argel Arghel Herb, root TM (colds, indigestion, NA Egypt, Sudan, 5, 9
(Delile) Hayne skin ailments, eye lotion) Algeria, Chad,
(Apocynaceae) Libya
Stachys aegyptiaca Pers. Egyptian stachys Herb TM (antispasmodic, NA Egypt 3
(Lamiaceae) antiallergenic); ES
Sterculia setigera Delile Karaya Exudate; roots, Exudate (gum, gum EA South Sudan 1
(Malvaceae) bark karaya); TM (roots, bark: (gum); Benin,
diversity of uses) Cameroon, Central
African Republic,
Senegal
Strophanthus gratus (Wall. Strophanthus, poison Seeds; root, stem CH (seeds: ouabain, a WA, CA Cameroon, 1, 6, 8, 10,
& Hook.) Baill. arrow vine leaf cardiotonic); TM Nigeria, Gabon 11
(Apocynaceae) (gonorrhea and numerous
other uses)
Strophanthus hispidus DC. Brown strophanthus Seeds CH: strophanthidine CA, EA Many countries 1, 6
(Apocynaceae) glycosides (chronic heart
weakness)
Strophanthus kombe Oliv. Strophanthus Seeds CH (k-strophanthin: CA, SA, Madagascar 2, 6
(Apocynaceae) cardiotonic) EA
B.-E. Van Wyk
African countries
Sutherlandia – see Lessertia
Synsepalum dulcificum Miracle fruit, Fruit CH (miraculin: sweetener; WA Congo, Ghana 1, 11
(Schum. & Thonn.) Daniell miraculous berry taste modifier)
(Sapotaceae)
Syzygium cordatum Hochst. Water berry, umdoni Bark TM (diarrhea, stomach SA, EA Kenya, South 1, 2
(Myrtaceae) ailments and other uses) Africa, Zimbabwe
Tabernaemontana crassa Adam’s apple flower Bark TM (many uses) WA, CA Regional trade 1, 6
Oliv. (Apocynaceae) (many countries)
Tabernanthe iboga Stapf Iboga Root CH (ibogaine, a stimulant CA, WA Gabon, Cameroon, 1, 6
(Apocynaceae) used for the treatment of Central African
drug addiction); TM Republic, Congo,
(hallucinogen, aphrodisiac, D.R. Congo,
asthenia; conjunctivitis and Equatorial Guinea
other ailments)
Tamarindus indica L. Tamarind Fruit pulp DS (fruit pulp: mild EA Chad, Ethiopia, 1, 2, 10
(Fabaceae) laxative, general tonic); South Sudan
TM (roots, bark, leaves:
diverse uses)
Tamarix gallica L. French tamarisk, Leaves, bark TM (astringent, tonic, liver NA Morocco, Algeria 5
(Tamaricaceae) saltcedar stimulant, stomachic)
Tanacetum cinerariifolia Pyrethrum Flower heads CH (source of pyrethrins, Balkans Cultivated: Kenya, 10
(Trevir.) Sch.Bip. natural insecticides) Tanzania
(=Chrysanthemum
cinerariifolium (Trevir.)
Vis.* (Asteraceae)
Taverniera abyssinica Rich. Taverniera, dingetegna Root TM (headache, stomach EA Ethiopia
(Fabaceae) ache, fever – root chewed)
Terminalia sericea Burch. Silver cluster-leaf, Roots, leaves, TM (general tonic, SA, EA Botswana, 1, 2, 8
ex DC. (Combretaceae) mususu bark diarrhea, many other Mozambique,
ailments) South Africa,
Zimbabwe
47
(continued)
48
Table 1 (continued)
African countries
Tetraclinis articulata (Vahl.) Arar tree Leaves, fruits, TM (numerous ailments); NA Morocco, Algeria, 5
Masters (Cupressaceae) wood, resin ES: distilled from Tunisia
sandarac(h) or gum
sandarac (the resin)
Tetradenia riparia (Hochst.) Iboza, umuravumba Leaves TM (coughs, colds, CA, SA, Burundi, 1, 2, 4
Codd (Lamiaceae) bronchitis, respiratory EA D.R. Congo,
ailments, diarrhea, angina, Rwanda, South
venereal diseases) Africa, Zimbabwe
Tetrapleura tetraptera Aridan Fruits, bark DS (fruit: spice, tonic); TM CA, WA, Cameroon, Congo, 1
(Schumach. & Thonn.) (fruit, bark: numerous EA D.R. Congo,
Taub. (Fabaceae) ailments) Equatorial Guinea,
Gabon, Ivory
Coast, Senegal,
Togo, Uganda
Teucrium polium L. Felty germander Herb TM (dysmenorrhea, NA Egypt, Tunisia 5
(Lamiaceae) digestive ailments,
antispasmodic, skin
allergy)
Thaumatococcus daniellii Miraculous fruit, ewe Seed aril CH (source of thaumatin, a WA, CA Côte d’Ivoire, 1
(Bennett) Benth. eran protein sweetener) D.R. Congo,
(Marantaceae) Nigeria
Thymbra capitata (L.) Cav. Capitate thyme Herb TM (various ailments); ES NA Tunisia (widely 5
[=Coridothymus capitatus (antiseptic, antispasmodic) cultivated)
(L.) Reichenb.f.]
(Lamiaceae)
B.-E. Van Wyk
African countries
Thymus decussatus Benth. Sinai thyme Herb TM (antispasmodic, NA Egypt 3
(Lamiaceae) toothache); ES
Thymus satureioides Coss. Moroccan wild thyme Herb TM (topical anti- NA Morocco 5
& Ball. (Lamiaceae) inflammatory); ES
(aromatherapy)
Toddalia asiatica (L.) Lam. Orange climber, Lopez Leaves, roots TM (antimalarial, EA, CA, Uganda, Gabon, 1, 2, 4, 8
(Rutaceae) root anti-inflammatory, SA, Ma Kenya,
spasmolytic) Madagascar, South
Africa, Tanzania
Tribulus terrestris L. Caltrops Fruits TM (urinary disorders and PA North African 1, 2, 5
(Zygophyllaceae) many other uses); countries
nutritional supplement
Trichilia emetica Vahl Cape/Natal mahogany Bark; seeds TM (bark: many uses); PA Ethiopia, 1, 2, 8
(Meliaceae) seeds: cosmetic oil (mafura Mozambique,
butter, mafoureira tallow) South Africa,
Zimbabwe
Trigonella foenum-graecum Fenugreek Seeds DS, PH (hypoglycaemic, EA Ethiopia, South 1, 2, 7, 10,
L. (Fabaceae) cholesterol-lowering); TM Sudan 11

(convulsions, stomach
disorders; topical for
ulcers)
Tylosema esculentum Marama bean Seeds DS (seeds); seed oil SA Namibia
(Burch.) Schreib. (edible, cosmetics)
(Fabaceae)
Urera acuminata Sampivato Root TM (gynecological use) Ma Madagascar 4
Gaudichaux (Urticaceae)
Vepris madagascarica Anzéty Bark, leaves ES (anethole) Ma Madagascar 9
(Baill.) H.Perrier (Rutaceae)
(continued)
49
50
Table 1 (continued)
African countries
Vernonia amygdalina Bitter leaf Leaves, roots TM (general tonic, PA D.R. Congo, 1, 2, 8
Delile, V. colorata (Willd.) numerous uses) Ethiopia, Kenya,
Drake (Asteraceae) Mozambique,
Nigeria, Rwanda,
Tanzania, Uganda,
Zimbabwe
Visnaga daucoides Gaertn. Khella Fruit TM; ES NA Morroco, Egypt 1, 7, 11
[=Ammi visnaga (L.) Lam.]
(Apiaceae)
Vitellaria paradoxa Karate, shea tree, shea Seeds DS (seed oil: edible shea WA, CA Burkina Faso, 1
Gaertn.f. (=Butyrospermum butter tree butter, cosmetics); TM Cameroon, Chad,
parkii Kotschy) (leaf, bark: several uses) Mali, Nigeria
(Sapotaceae)
Voacanga africana Stapf Voacanga Seeds CH (isolation of PA Cameroon, 1, 6, 8
and V. thouarsii (Rubiaceae) tabersonine; synthesis of Madagascar; also
vincamine, a cerebral Côte d’Ivoire,
stimulant); TM (bark, Ghana, D.R. Congo,
roots: many uses) Sao Tome and
Principe, Tanzania
Warburgia salutaris Pepperbark tree Bark, leaves TM (bark: antibiotic, many EA, SA South Africa, 1, 2, 8, 10,
(G. Bertol) Chiov. (=W. uses; leaves: tablets, spice) Kenya, Madagascar, 11
ugandensis Sprague) Mozambique,
(Canellaceae) Tanzania
Withania somnifera (L.) Indian ginseng, Roots PH (physical and Co, PA Almost all 1, 2, 4, 6, 10
Dunal (Solanaceae) ashwagandha psychological stress); TM countries
(roots, leaves: many uses,
B.-E. Van Wyk
also topical for sores)

African countries
Ximenia americana L. Small sourplum Seeds Seed oil: cosmetic; TM PA Ethiopia, 1, 2
(Olacaceae) (roots, leaves: many uses) Madagascar,
Mozambique,
Namibia
Xylopia aethiopica (Dunal) Guinea pepper Fruits Fruits: essential oil, spice; CA, WA Benin, Cameroon, 1, 8
A.Rich. (Annonaceae) ES (flavourant) TM (fruits, Central African
leaves, bark: diverse uses) Republic, Congo,
Equatorial Guinea,
Sierra Leone
Xysmalobium undulatum Uzara, ishongwe Roots TM/PH (diarrhea, SA, EA Namibia, South 1, 7, 8, 9, 10,
(L.) Aiton f. (Apocynaceae) dysentery, dysmenorrhea, Africa, Zimbabwe 11
colic; root powder:
headache)
Zanthoxylum zanthoxyloides Senegal prickly-ash Roots, bark, TM (many ailments); DS WA Côte d’Ivoire, 9
(Lam.) Zepern. & Timler leaves (roots: sickle cell anaemia) Mali, Burkina
(Rutaceae) Faso, Ghana,
Nigeria
Ziziphus mucronata Willd. Buffalo thorn, Bark (roots, TM (numerous ailments, WA, EA, Many African 1, 2
(Rhamnaceae) mokgalo leaves, fruits) including stomach and SA countries
urinary tract; topically for
ulcers)
The list includes 258 species (246 indigenous and 12 naturalised and/or commonly cultivated)
Main uses: PH phytomedicine, DS dietary supplement and/or functional food, CH source of a useful chemical compound(s), ES source of essential oil, TM
traditional medicine
Origin (following Van Wyk 2015): NA North Africa, WA West Africa, EA East Africa, SA Southern Africa, PA Pan-African, Ma Madagascar and Indian Ocean
Islands, Co Cosmopolitan, Cu cultigen of unknown/uncertain origin
General references (1–11): 1 = Neuwinger (2000), 2 = Arnold et al. (2002), 3 = Vasisht and Kumar (2004), 4 = Gurib-Fakim and Brendler (2004), 5 = IUCN
(2005), 6 = Schmelzer and Gurib-Fakim (2008) [PROTA 11(1)], 7 = Wichtl (2009), but see also Wichtl and Bisset (2000), 8 = African Herbal Pharmacopoeia
(Brendler et al. 2010), 9 = Gurib-Fakim and Schmelzer (2013) [PROTA 11(2)], 10 = Van Wyk et al. (2015), 11 = Van Wyk and Wink (2015)
Exotic (non-African) taxa are indicated with an asterisk (*)
51
52 B.-E. Van Wyk
Table 1 provides a brief overview of the most important species of African ori-
gin, their main uses and region(s) of origin. The list includes products of interna-
tional trade, such as phytomedicines (Fig. 1), traditional medicines (Fig. 2), dietary
supplements (Fig. 3), and sources of pure chemical entities (Fig. 4) and essential oil
(Fig. 5), but also some species of regional or local importance. The choice of species
is partly based on the statistics provided by Van Wyk (2015), who used a combina-
tion of Google Scholar and Scopus to determine the relative popularity and scien-
tific interest in 175 species from sub-Saharan Africa. It is also based on 11 general
publications and reviews that have appeared since 2000, namely Neuwinger (2000),
Arnold et al. (2002), Vasisht and Kumar (2004), Gurib-Fakim and Brendler (2004),
IUCN (2005), Schmelzer and Gurib-Fakim (2008), Wichtl (2009), (Brendler et al.
2010), Gurib-Fakim and Schmelzer (2013), Van Wyk et al. (2015) and Van Wyk and
Wink (2015). These publications can be consulted for detailed information on the
botany, chemistry, traditional uses and biological activities of the plants.
Fig. 1 Examples of African phytomedicines. 1, Centella asiatica leaves; 2, Griffonia simplicifolia

seeds; 3, Harpagophytum procumbens (3a, leaves and flowers; 3b, fruit; 3c, sliced and dried sec-
ondary roots); 4, Pelargonium sidoides (4a, flowering plant; 4b, flowers; 4c, fresh roots); 5, Prunus
africana (5a, leaves and fruits; 5b, bark); 6, Sceletium tortuosum (6a, flowering plant; 6b, tradi-
tional dried product); 7, Trigonella foenum-graecum (7a, leaves, flower and fruit; 7b, seeds); 8,
Withania somnifera (8a, leaves, flowers and fruits; 8b, roots); 9, Xysmalobium undulatum (9a,
leaves and flowers; 9b, dried roots) (Copyright: B.-E. Van Wyk)
A Review of African Medicinal and Aromatic Plants 53
Fig. 2 Examples of African medicinal plants. 1, Aloe ferox (1a, flowering plant; 1b, from top to
bottom: spray-dried leaf juice, powdered aloe lump, aloe lump); 2, Bulbine frutescens (2a, flower-
ing plant; 2b, flowers; 2c, leaves showing gel); 3, Catha edulis (3a, flowers; 3b, fresh leaves); 4,
Harungana madagascariensis leaves and fruits; 5, Kigelia africana flowers and fruit; 6, Lessertia
frutescens (6a, flowers and fruits; 6b, dried leaves); 7, Mondia whitei (7a, flowers; 7b, dried roots
and powdered root); 8, Phytolacca dodecandra flowers; 9, Securidaca longepedunculata (9a,
leaves and flowers; 9b, root bark); 10, Senna alexandrina (10a, leaves and flowers; 10b, dried
leaves and fruits); 11, Warburgia salutaris leaves and flower (Copyright: B.-E. Van Wyk)
ources of Information on African Medicinal and Aromatic

S
Plants
Medicinal plants have been used in Africa since ancient times but the documenta-
tion of the knowledge generally dates back to the last two centuries. Colonial floras
are often viewed as inventories of plants with potential commercial value and these
publications are still of considerable historical value. Major contributions made dur-
ing the twentieth century were those of Burkill (1937, 1985–2004) for West Africa
and Watt and Breyer-Brandwijk (1962) for southern and eastern Africa. General
works include Sofowora (1982) and Iwu (1993, 2014), as well as the first African
pharmacopoeia in two volumes (African Pharmacopoeia 1981, 1986). Other note-
worthy publications are Boulos (1983) for North Africa; Ayensu (1978), Oliver-
Bever (1986) and the Ghana Herbal Pharmacopoeia (1992) for West Africa;
Kokwaro (1993) and Abebe and Ayehu (1993) for East Africa, and Gelfand et al.
(1985), Hedberg and Staugård (1989), Hutchings et al. (1996) and Van Wyk et al.
(1997) for southern Africa.
The year 2000 marked a turning point in the tempo of publications on African
medicinal and aromatic plants. Neuwinger (2000) made a major contribution by
compiling all available knowledge on sub-Saharan Africa, which incorporated many
54 B.-E. Van Wyk
Fig. 3 Examples of African dietary supplements and functional foods. 1, Acanthosicyos horridus
(1a, female plant; 1b, fruit and seeds); 2, Adansonia digitata (2a, tree; 2b, fruit, seeds and fruit
pulp); 3, Aloe vera (3a, plants; 3b, flowers; 3c, fresh gel and spray-dried gel); 4, Aspalathus lin-
earis (4a, plant; 4b, leaves, flowers and fruits; 4c, rooibos tea (fermented and green)); 5, Balanites
aegyptiaca (5a, tree; 5b, leaves and thorns; 5c, fruits); 6, Combretum micranthum (whole and cut
leaves); 7, Cyclopia genistoides (7a, leaves and flowers; 7b, cut stems and leaves); 8, Hibiscus
sabdariffa (8a, flower; 8b, dried calyces); 9, Hoodia gordonii (9a, flowering plant; 9b, stems); 10,
Hypoxis hemerocallidea (10a, flowering plant; 10b, corms); 11, Lepidium sativum (11a, plants;
11b, flowers and fruits; 11c, seeds); 12, Moringa stenopetala (12a, tree; 12b, flowers; 12c, leaves);
13, Sesamum indicum (13a, leaves and flower; 13b, seeds); 14, Tamarindus indica (14a, tree; 14b,
leaves and fruits; 14c, fruits; 14d, fruit pulp) (Copyright: B.-E. Van Wyk)
Fig. 4 Examples of African sources of chemical compounds and extracts. 1, Acacia senegal (1a,
leaves and fruits; 1b, gum); 2, Catharathus roseus flowers; 3, Coffea arabica (3a, plant; 3b, flower
and fruits); 4, Gloriosa superba; 5, Pausinystalia johimbe (5a, leaves; 5b, bark); 6, Physostigma
venenosum (6a, leaves; 6b, seeds); 7, Rauvolfia vomitoria leaves and fruits; 8, Sclerochiton ilicifo-
lia; 9, Strophanthus gratus; 10, Vitellaria paradoxa seed oil (shea butter); 11, Voacanga africana
seeds (Copyright: B.-E. Van Wyk)
poorly known publications of the twentieth century, including those from French-
speaking regions of the continent. An ambitious project to document the useful
plants of tropical Africa was initiated in 2000. This was the PROTA program (Plant
Resources of Tropical Africa), aimed at treating 8681 useful plant species (PROTA
2010a) in separate commodity groups (see http://www.prota4u.org). Medicinal
plants have been published in two volumes (out of a planned total of four), namely
volumes 11(1) and 11(2) of the PROTA series. No less than 894 primary use species
were included in Volume 1 (Schmelzer and Gurib-Fakim 2008), while 409 species
were included in 146 reviews in Volume 2 (Gurib-Fakim and Schmelzer 2013).
Another useful book dealing with the medicinal and aromatic plants of the various
countries and regions of the African continent (Vasisht and Kumar 2004) was pub-
lished by the United Nations Industrial Development Organization (UNIDO).
Detailed information on the chemistry and biological activity of African plants can
also be obtained from the database developed by Ermias Dagne at Addis Ababa
University (Natural Database for Africa 2011). Of special interest is the African
herbal pharmacopoeia (Brendler et al. 2010), representing a collective effort from
several African scientists which comprises monographs of 51 carefully selected
African medicinal plants.
56 B.-E. Van Wyk
Fig. 5 Examples of African aromatic plants. 1, Aframomum melegueta seeds; 2, Agathosma betu-
lina (2a, leaves and flower; 2b, dried leaves); 3, Artemisia afra leaves and flower heads; 4,
Boswellia neglecta (4a, leaves and fruits; 4b, gum-resin); 5, Boswellia papyrifera (5a, tree; 5b, leaf
and flowers; 5c, gum-resin); 6, Boswellia rivae gum-resin; 7, Boswellia sacra (7a, leaves; 7b, gum-
resin); 8, Commiphora guidottii gum-resin; 9, Commiphora myrrha (9a, leaves and fruits; 9b,
gum-resin); 10, Commiphora wildii gum-resin; 11, Lippia javanica leaves and flower heads; 12,
Myriothamnus flabellifolius leaves and flowers; 13, Pelargonium cv. Rosé; 14, Siphonochilus aeth-
iopicus (14a, flowering plant; 14b, rhizomes); 15, Xylopia aethiopica dried fruits (Copyright:
B.-E. Van Wyk)
Books and publications with a global scope also included several African species
of commercial relevance. Twelve of a total of 118 species (i.e. more than 10%)
treated in the four volumes of the WHO Selected Monographs of Medicinal Plants
(WHO 1999, 2003, 2007, 2009) are indigenous to Africa, namely Aloe ferox, A.
vera, Ammi majus, Centella asiatica, Commiphora myrrha (as C. molmol),
Harpagophytum procumbens, Momordica charantia, Prunus africana, Ricinus
communis, Senna alexandrina (as Cassia senna), Visnaga daucoides (as Ammi vis-
naga) and Withania somnifera. Aloe vera is an ancient cultigen of North African and
Arabian origin, while Ricinus communis is almost certainly of Ethiopian origin,
given the extreme morphological variation in this region (Van Wyk 2015).
Another global review of medicinal plants was published by Van Wyk and Wink
(2004). In the most recent revised edition of this book (Van Wyk et al. 2015; Van
Wyk and Wink 2017), 36 of the 350 monographs (roughly 11%) represent African
plants. The summary table of 911 species shows 111 species of African origin
(roughly 12%).
Seven African species were listed amongst the 43 phytomedicines presented by
Van Wyk and Wink (2015), namely Centella asiatica, Griffonia simplicifolia,
Harpagophytum procumbens, Pelargonium sidoides, Prunus africana, Trigonella
foenum-graecum and Withania somnifera. In addition, Sceletium tortuosum and
Xysmalobium undulatum may also qualify for inclusion in the list of phytomedi-
cine. Ten of the 56 species (nearly 18%) listed as functional foods and nutraceuti-
cals are indigenous to Africa: Adansonia digitata, Aloe ferox, Aspalathus linearis,
Hibiscus sabdariffa, Hoodia gordonii, Hypoxis hemerocallidea, Lepidium sativum,
Sesamum indicum, Synsepalum dulcificum and Tamarindus indica. Thirteen of the
135 species (roughly 10%) listed as sources of commercially relevant chemical
compounds are African: Catharanthus roseus, Centella asiatica, Coffea arabica,
Cissampelos pareira, Cyclopia genistoides, Pausinystalia johimbe, Physostigma
venenosum, Sclerochiton ilicifolius, Strophanthus gratus, Synsepalum dulcificum,
Thaumatococcus daniellii, Voacanga africana and V. thouarsii.
Numerous other scientific publications and books provide additional insights
into the most popular and important African medicinal and aromatic plants (Van
Wyk 2015, and references cited therein). These include the treatment of 25 African
plants with exceptional potential, published by PROTA (2010b), IUCN (2005) for
North Africa, the Nigerian Natural Medicine Development Agency (2004, 2006) for
West Africa.
Hedberg et al. (2009) and Asfaw and Demissew (2009) for East Africa, Latham
and Konda ku Mbuta (2014) for Central Africa, Van Wyk and Gericke (2000), Von
Koenen (2001), Arnold et al. (2002) and Van Wyk et al. (2009) for Southern African
and Gurib-Fakim and Brendler (2004) for Madagascar and the Indian Ocean Islands.
Surveys of the informal and unregulated markets that exist in African countries are
a valuable source of information on the most popular medicinal plants and the large
volumes of material that are traded. Examples include Cunningham (1988), Mander
(1998), Williams et al. (2000) and Von Ahlefeldt et al. (2003) for South Africa, Van
Andel et al. (2012) for Ghana, Jusu and Sanchez (2014) for Sierra Leone, Quiroz
et al. (2014) for Benin, Towns et al. (2014) for Gabon, Otieno et al. (2015) for
Tanzania and Randriamiharisoa et al. (2015) for Madagascar.
Conclusions and Future Prospects
Although some African medicinal and aromatic plants have become important items
of international trade, the full diversity and commercial potential of the entire flora
have yet to be explored. The strong scientific interest in African plants, as reflected
in the large numbers of recent publications, indicates an awareness of the potential
for developing novel treatments and for making new discoveries that can be of ben-
efit to mankind. Much remains to be discovered, especially in poorly known plants
from remote regions of the African continent. The botanical literature, incomplete
58 B.-E. Van Wyk
as it may be, already provides a glimpse of the exciting research opportunities that
are presented by the rich diversity of useful African plants. Traditional uses are
based on oral-traditional knowledge that has been passed down through many gen-
erations. This cultural context adds to the complexity of the patterns of plant use and
is particularly relevant to understanding the origins of medicinal plant use and aro-
matherapy as practiced by our early ancestors in Africa. The available wisdom and
experience should not only be studied and documented but also used to inspire new
innovations. It seems reasonable to expect that African medicinal and aromatic
plants will gradually rise to more international prominence and that they will even-
tually rival the large number of herbal products of European and Asian origin. The
rapid development of more sophisticated technologies will open up new frontiers of
scientific exploration. It can be anticipated that new tools and more holistic
approaches will become available for gaining insights into the efficacy and pharma-
cological activities of many of the popular African plants that have not yet been
subjected to rigorous scientific study.
Acknowledgements Thanks are due to the University of Johannesburg and the National Research
Foundation of South Africa for providing logistic and financial support to the National Research
Chair in Indigenous Plant Use.
References
Abebe D, Ayehu A (1993) Medicinal plants and enigmatic health practices of Northern Ethiopia.
B.S.P.E, Addis Ababa
African Pharmacopoeia (1981, 1986) (vols 1, 2). OAU/STRC Publications Division, Laos
Arnold TH, Prentice GA, Hawker LC, Snyman EE, Tomalin M, Crouch NR, Pottas-Bircher C
(2002) Medicinal and magical plants of southern Africa: an annotated checklist. Strelitzia 13.
National Botanical Institute, Pretoria
Asfaw N, Demissew S (2009) Aromatic plants of Ethiopia. Shama Books, Addis Ababa
Ayensu ES (1978) Medicinal plants of West Africa. Reference Publications Inc, Algonac
Boulos L (1983) Medicinal plants of North Africa. Algonac, Michigan
Brendler T, Eloff JN, Gurib-Fakim A, Phillips LD (eds) (2010) African herbal pharmacopoeia.
Association of African Medicinal Plant Standards, Port Louis
Burkill HM (1985–2004) The useful plants of West Tropical Africa, 2nd edn, 6 vols. Royal Botanic
Gardens, Kew
Cunningham AB (1988) An investigation of the herbal medicine trade in Natal/KwaZulu.
Investigational Report No. 29. Institute for Natural Resources, University of KwaZulu-Natal,
Pietermaritzburg
Dobignard A, Chatelain C (2010–2013) Index synonymique flore d’Afrique du Nord, vols 1–5.
Conservatoire et jardin Botaniques, Genève
Gelfand M, Mavi S, Drummond RB, Ndemera B (1985) The traditional medicinal practitioner in
Zimbabwe. Mambo Press, Gweru
Ghana Herbal Pharmacopoeia (1992) Policy Research and Strategic Planning Institute, Accra
Gurib-Fakim A, Brendler T (2004) Medicinal and aromatic plants of Indian Ocean islands:
Madagascar, Comores, Seychelles and Mascarenes. Medpharm Scientific Publishers, Stuttgart
Gurib-Fakim A, Schmelzer GH (eds) (2013) The plant resources of Tropical Africa 11(2),
Medicinal Plants 2. Wageningen, CTA PROTA
Hedberg I, Staugård F (1989) Traditional medicinal plants: traditional medicine in Botswana.

Ipeleng, Gaborone
Hedberg I, Friis I, Persson E (2009) Flora of Ethiopia and Eritrea, vol 8. The National Herbarium,
Addis Ababa University/Department of Systematic Botany, Uppsala University, Addis Ababa/
Uppsala
Hutchings A, Scott AH, Lewis G, Cunningham A (1996) Zulu medicinal plants. Natal University
Press, Pietermaritzburg
IUCN (2005) A guide to medicinal plants in North Africa. International Union for Conservation of
Nature and Natural Resources, Málaga
Iwu MM (1993) Handbook of African medicinal plants. CRC Press, Boca Raton
Iwu MM (2014) Handbook of African medicinal plants, 2nd edn. CRC Press, Boca Raton
Jusu A, Sanchez AC (2014) Medicinal plant trade in Sierra Leone: threats and opportunities for
conservation. Econ Bot 68:16–29
Klopper RR, Chatelain C, Bänninger V, Habashi C, Steyn HM, De Wet BC, Arnold TH, Gautier
L, Smith GF, Spichiger R (2006) Checklist of the flowering plants of Sub-Saharan Africa. An
index of accepted names and synonyms. South African Botanical Diversity Network Report
No. 42. SABONET, Pretoria
Kokwaro JO (1993) Medicinal plants of East Africa, 2nd edn. East Africa Literature Bureau,
Nairobi
Latham P, Konda ku Mbuta A (2014) Useful plants of Bas-Congo Province, Democratic Republic
of Congo, 2nd edn, 2 vols. Published by the Authors
Madagascar Catalogue (2013) Catalogue of the vascular plants of Madagascar. Missouri Botanical
Garden, St. Louis, U.S.A. & Antananarivo, Madagascar. http://www.efloras.org/madagascar.
Accessed May 2013
Mander M (1998) Marketing of indigenous medicinal plants in South Africa: a case study in
KwaZulu-Natal. Food and Agricultural Organization of the United Nations, Rome
Manning J, Goldblatt P (2012) Plants of the Greater Cape Floristic Region 1: The Core Cape Flora.
Strelitzia 29. South African National Biodiversity Institute, Pretoria
Natural Database for Africa (2011) Version 2.0. NDA is a species based versatile database devel-
oped by Ermias Dagne, Ethiopia ([email protected])
Neuwinger HD (2000) African traditional medicine – a dictionary of plant use and applications.
Medpharm Scientific Publishers, Stuttgart
Nigerian Natural Medicine Development Agency (2004) Medicinal plants of Nigeria: South-West
Nigeria. Nigerian Natural Medicine Development Agency, Lagos
Nigerian Natural Medicine Development Agency (2006) Medicinal plants of Nigeria: North
Central Zone. Nigerian Natural Medicine Development Agency, Lagos
Oliver-Bever B (ed) (1986) Medicinal plants of tropical West Africa. Cambridge University Press,
Cambridge
Otieno J, Abihudi S, Veldman S, Nahashon M, Van Andel T (2015) De Boer HJ (2015) Vernacular
dominance in folk taxonomy: a case study of ethnospecies in medicinal plant trade in Tanzania.
J Ethnobiol Ethnomed 11:10. doi:10.1186/1746-4269-11-10
Prota (2010a) The plant resources of tropical Africa. Updated list of species and commodity group-
ings. Backhuys Publishers and CTA PROTA, Wageningen
Prota (2010b) Review of 25 African plants with special potential. Backhuys Publishers and CTA
PROTA, Wageningen
Quiroz D, Towns A, Legba SI, Swier J, Brière S, Sosef M, Van Andel T (2014) Quantifying the
domestic market in herbal medicine in Benin, West Africa. J Ethnopharmacol 151:1100–1108
Randriamiharisoa MN, Kuhlman AR, Jeannoda V, Harison Rabarison H, Rakotoarivelo N,
Randrianarivony T, Raktoarivony F, Randrianasolo A, Bussmann RW (2015) Medicinal plants
sold in the markets of Antananarivo, Madagascar. J Ethnobiol Ethnomed 11:60. doi:10.1186/
s13002-015-0046-y
Royal Botanic Gardens Kew (2016) The state of the world’s plants report – 2016. Royal Botanic
Gardens, Kew. ISBN:978-1-84246-628-5
60 B.-E. Van Wyk
Schmelzer GH, Gurib-Fakim A (eds) (2008) The plant resources of tropical Africa 11(1), Medicinal
Plants 1. Backhuys Publishers and CTA PROTA, Wageningen
Sofowora A (1982) Medicinal plants and traditional medicine in Africa. Wiley, Chichester
The Plant List (2013) The plant list. A working list of all plant species, version 1.1. Royal Botanic
Gardens, Kew and Missouri Botanical Garden. http://www.theplantlist.org/
Towns AM, Quiroz D, Guinee L, De Boer H, Van Andel T (2014) Volume, value and floristic diver-
sity of Gabon’s medicinal plant markets. J Ethnopharmacol 155:1184–1193
Van Andel T, Myren B, Van Onselen S (2012) Ghana’s herbal market. J Ethnopharmacol
140:369–378
Van Wyk B-E (2015) A review of commercially important African medicinal plants.
Van Wyk B-E, Gericke N (2000) People’s plants – a guide to useful plants of Southern Africa.
Briza Publications, Pretoria
Van Wyk B-E, Wink M (2004) Medicinal plants of the world. Timber Press/Briza Publications,
Portland/Pretoria
Van Wyk B-E, Wink M (2015) Phytomedicines, herbal drugs and poisons. Kew Publishers, Kew
and University of Chicago Press, Chicago
Van Wyk B-E, Wink M (2017) Medicinal plants of the world, 2nd edn. CABI/Briza Publications,
Wallingford/Pretoria
Van Wyk B-E, Van Oudtshoorn B, Gericke N (1997) Medicinal plants of South Africa. Briza
Publications, Pretoria
Van Wyk B-E, Van Oudtshoorn B, Gericke N (2009) Medicinal plants of South Africa, 2nd edn.
Briza Publications, Pretoria
Van Wyk B-E, Wink C, Wink M (2015) Handbuch der Arzneipflanzen. Wissenschaftliche
Verlagsgesellschaft, Stuttgart
Vasisht K, Kumar V (2004) Compendium of medicinal and aromatic plants, vol 1. Trieste,
ICS-UNIDO
Von Ahlefeldt D, Crouch NR, Nichols G, Symmonds R, McKean S, Sibiya H, Cele MP (2003)
Medicinal plants traded on South Africa’s eastern seaboard. Ethekwini Parks Department and
University of Natal, Durban
Von Koenen E (2001) Medicinal, poisonous and edible plants in Namibia. Klaus Hess Publishers,
Windhoek/Göttingen
Watt JM, Breyer-Brandwijk MG (1962) The medicinal and poisonous plants of Southern and
Eastern Africa, 2nd edn. Livingstone, London
WHO Monographs on selected medicinal plants, (vols 1–4) (1999, 2003, 2007, 2009) World
Health Organisation, Geneva
Wichtl M (2009) Teedrogen und Phytopharmaka. Wissenschaftliche Verlagsgesellschaft, Stuttgart
Wichtl M, Bisset NG (eds) (2000) Herbal drugs and phytopharmaceuticals. CRC Press, Boca
Raton
Williams VL, Balkwill K, Witkowski ETF (2000) Unravelling the commercial market for medici-
nal plants and plant parts on the Witwatersrand, South Africa. Econ Bot 54:310–327
Traditional Use of Medicinal and Aromatic
Plants in Africa
Olufunke O. Fajinmi, Olaoluwa O. Olarewaju, and J. Van Staden
Abstract Traditional medicine and the use of medicinal and aromatic plants in
Africa are linked to social life, culture and daily activities of the African people. The
celebration of life events involve the presentation and sharing of several culturally
significant items such as honey, salt, Cola and other medicinal plants and their prod-
ucts. In this chapter, the rich and unique culture of African countries is discussed in
relation to the use of medicinal and aromatic plants. As a special feature, the major
medicinal and aromatic plants endemic to Western, Northern and Southern Africa
are reviewed with regards to their manifold uses. The disposition of African people
to traditional medicine, formulation of policies to support the growth of traditional
medicine and the progress made so far are highlighted. As a result of the acceptance
of African traditional medicine by a vast majority of African populace and support
gained from the African Union, World Health Organization and the United Nations;
African traditional medicine will in the near future be highly valued just like the
Chinese traditional medicine system.
Keywords African culture • Traditional medicine • Aloe species • Wonder nut

(Cola nitida) • Henna • Marula • Acalypha species • Aromatic plants • Buchu
1 Africa, Its People, Culture and Traditional Medicine
Africa is one of the seven continents of the world, the second largest after Asia, with
about a billion people of diverse tribes, language, tradition and culture. It comprises
54 countries, with a total land area of 30 Million Square Kilometers. Africa, as a
O.O. Fajinmi • J. Van Staden (*)

Research Centre for Plant Growth and Development, School of Life Sciences, University of
KwaZulu-Natal, Private Bag X01, Scottsville, Pietermaritzburg 3209, South Africa
O.O. Olarewaju
Department of Horticultural Science, School of Agricultural,
Earth and Environmental Science, University of KwaZulu-Natal,
Private Bag X01, Scottsville, Pietermaritzburg 3209, South Africa

DOI 10.1007/978-94-024-1120-1_3
62 O.O. Fajinmi et al.
continent, is rich in various and interesting cultures and as such is regarded as the
most diverse amongst the seven continents of the world. These cultures and tradi-
tions have been passed from one generation to another and are still maintained to
date, irrespective of colonization and civilization.
One of the most important aspects of African culture is its use of traditional
medicine (TM) or medicinal plants (MP) which is an ancient practice. African TM
is one of the oldest and unique systems across the world. It is diverse, as it is shaped
by cultural beliefs of different countries in Africa. Some African countries have
similarities in the use and practice of TM and therefore utilize similar plants for the
cure of diseases and infections. This, however, is common in countries with linked
histories. For example, some African countries were formed as a result of migration
from another African country during wars and tribal conflicts arising from royal
battles and land ownership. This migration to exile led to the birth of subtribes,
tribes and countries. While in exile, diseases and infections were treated either with
plants found in their place(s) of refuge or dried MP taken from their home country.
Furthermore, this similarity often arises from the common diseases that affected
certain regions of Africa. These have been the cause of high mortality. For example,
malaria in West Africa, tuberculosis in the Southern African Development
Community (SADC) and North Africa, dengue fever in East Africa and hepatitis in
Northern Africa. Although, treatment for these diseases have been developed in
clinical practice, MP endemic to Africa offer varieties of bioactive compounds
capable of addressing such health issues at an affordable price. In addition to afford-
ability, TM is more accessible, as compared to orthodox medicine. Hence, more
preferred by most African people, especially those living in the rural areas.
2 A
frica, a Continent Blessed with Plants
of High Medicinal Value
Africa is blessed with a vast amount of plant resources with an estimated number of
species between 40,000–45,000, out of which some 5000 species are used medici-
nally (Mahomoodally 2013). Several patents have been developed from MP endemic
to Africa and formulations (tinctures, teas and infusions) derived from African MP
are utilized both across Africa and the developed world. Some of the highly utilized
MPs constitute a crucial part of African pharmacopeias. Acacia senegal, Artemisia
herba-alba, Aspalathus linearis, Centella asiatica, Catharanthus roseus, Cyclopia
genistoides, Momordica charantia, and Pelargonium sidoides are some MP with
commercial importance that form part of the African herbal pharmacopeia
(Mahomoodally 2013). Apart from the above species, there are also several other
plants that are highly valued by African TM and revered as export materials which
add to the economy of several African nations. Some of these species are endemic
(e.g. Acalypha sp.) or are commonly found in specific regions of Africa (e.g. Aloe ssp.).
The Aloe species exist abundantly in SADC countries and are also medicinally
utilized extensively in these countries.
Traditional Use of Medicinal and Aromatic Plants in Africa 63
3 A
loe Species, an Integral Part of the Southern African
Traditional Medicine System
Aloe ferox, popularly known as Cape Aloe is revered in South African TM system
for its high medicinal value. It is regarded as the most common Aloe species in
South Africa and native to South Africa and Lesotho. It has been used since ancient
times and is one of the few plants found in San rock paintings (Mahomoodally
2013). Globally, it is a valuable raw material used in the pharmaceutical, food, and
cosmetic industries. Both aloe production and tapping form an important means of
livelihood in some rural communities of Southern Africa. The finished product
obtained from aloe tapping, aloe bitters, has remained an important South African
export product since 1761 when it was first exported to Europe (Mahomoodally 2013).
Till date, it has remained one of the highly utilized MPs within SADC countries
and beyond. Aloe is harvested extensively and used in various ways in Zambia and
Zimbabwe (Shumba et al. 2009) and other Southern African countries. The fleshy
leaf is the main part of the plant utilized for medicinal and cosmetic purposes. It is
sliced open and the fresh gelatinous juice is applied topically for the treatment of
wounds, rashes, skin irritation and burns or can be boiled and drunk as a cough
remedy (Shumba et al. 2009). Apart from the medicinal value of Aloe, it is also used
in preparing young girls for marriage ceremony (Shumba et al. 2009). Wedding is
an important traditional ceremony among Africans.
4 M
edicinal Plants Used During Wedding Ceremonies
in the West African Culture
In West Africa, traditional wedding takes place before the court wedding as the
couple are regarded as husband and wife once the traditional wedding has taken
place. Traditional wedding is done in a unique way in West Africa. During the event,
the elders of both families will be present together with the parents of the couples’
or their representatives if they are deceased. The use of certain MPs during the cer-
emony is common in almost all West African countries, as they constitute an impor-
tant part of the culture of these countries. Kola nuts (Cola nitida), nuts of bitter kola
(Garcinia kola) and alligator pepper (Aframomum meleguata) are frequently used
during traditional weddings across West Africa.
The genus Cola belongs to the family Sterculiaceae which is one of the most
important genera of this family. It is endemic to tropical Africa and has its greatest
diversity in West Africa (Asogwa et al. 2006). Kola nut is highly revered during
traditional ceremonies in West African culture. It has a unique place amongst West
Africans as a result of its specific importance in the social life and religious customs
of the people in the tropics of West Africa (Adebayo and Oladele 2012). It is an
important commodity presented before and during traditional wedding ceremonies
in West African culture. When the family of the groom sends Kola nuts to the family
of the bride and it is accepted, this act symbolizes their support and readiness for the
union of the couple. During the wedding ceremony, Kola nut is broken to seal the
friendship between the two families and blessings are pronounced on the couple. In
some West African communities, it is believed that marriages would crash if Kola is
not presented during the wedding ceremony.
Bitter kola (Garcinia kola) is an indigenous medicinal plant found in rain forest
of Central and Western African countries such as Benin Republic, Cameroon,
Democratic Republic of Congo (DRC), Cote d’Ivoire, Gabon, Ghana, Liberia,
Nigeria, Senegal and Sierra Leone (Esiegwu et al. 2014). Garcinia kola is cultivated
in West African countries for its edible fruit and seeds which serve as rejuvenating
agent for masticatory purposes (Ibiblio 1983). It is popularly chewed in Nigeria for
nervous alertness and induction of insomnia (Esiegwu et al. 2014). The nut of bitter
kola is mostly eaten to stimulate the flow of saliva (Omode et al. 1995; Leakey
1999) and, hence, are widely traded in the market.
The dried nut of bitter kola is ground and mixed with honey to make a mixture
used for cough treatment (Esiegwu et al. 2014). In West Africa, Garcinia kola dried
peels are used to treat cuts and sore throats, roots soaked in gin for tooth decay,
cough and gonorrhoea, tea (steamed leaves) for stomach ache, hepatitis, respiratory
tract inflammation, diarrhea and as an aphrodisiac, seed (nuts) for liver cirrhosis,
dried seed for asthma and fresh fruit as a broncho-dialator (Lewis 2001). The roots
of the plant are commonly used as chewing stick which are mostly sold in local
markets across West Africa (Iwu 2014).
Aframomum melegueta (grains of paradise, alligator pepper, melegueta pepper)
is an aromatic plant with edible spicy fruit. It is a tropical, herbaceous, perennial
plant belonging to the family Zingiberaceae (Doherty et al. 2010). The seeds have a
pungent peppery taste as a result of its aromatic ketones (Galal 1996). Aframomum
melegueta has both medicinal and nutritive values and is commonly found in rain
forests (Doherty et al. 2010). The plant is extensively utilized in West and Central
African TM systems (Iwu 2014). It is widely spread across tropical African coun-
tries such as Nigeria, Ghana, Cote D’Ivoire, Liberia, Cameroon, Sierra Leone,
Ghana and Togo (Doherty et al. 2010). In the southern part of Nigeria, it is chewed
with Kola nut as a masticatory agent. The fresh fruit is used as an aphrodisiac and
leaves for measles and leprosy (Iwu 2014). Alligator pepper is used in the produc-
tion of cosmetics and perfumes (Burkill 1985). During various traditional ceremo-
nies in Africa, bitter kola and alligator pepper are presented together with Kola nut.
5 W
onder Nut (Cola nitida) and Its Importance
in the Cultural, Social and Everyday Life
of the African People
Kola nut is presented when welcoming important guests, elders or a chief in Nigeria.
The significance of Kola nut among the Igbo people of Nigeria are: ushering in a
new day, divination, oath taking and welcoming of guests. Breaking of Kola nut is
believed to usher in peace and unity. During settlement of disputes between family
members or communities involved in land disputes, kola is presented to the repre-
sentatives of the families or communities before the meeting is commenced. The nut
when chewed helps to stay awake, quenches thirst and hunger. For this reason, it is
widely used in West African countries as an alternative to coffee drinking.
As a result of its ability to suppress sleep, kola nut is now popularly consumed
by higher institution students in West Africa. In the olden days, Kola nut was used
in exchange for money in some West African countries. The nut is also of great
economic importance as it is a cash crop of commercial importance which is culti-
vated for local consumption and export purposes like cocoa. The produce is con-
sumed locally and exported outside Africa.
There are other endemic plants used during ceremonies and festivals in Africa
such as Henna which is known globally for its use in the cosmetic industry.
6 Legendary Plants from North Africa
Henna (Lawsonia inermis): Its Cultural, Traditional

Importance and Its Cosmetic Use in Africa
The Henna plant, which is used across Africa during wedding ceremonies, is also
utilized in the cosmetic industry. Henna is known globally for its use as hair dye and
stain for the skin and nails. Lawsonia inermis Linn (Henna) belongs to the family
Lythreaceae with the genus Lawsonia as its sole member. It is a glabrous branched
shrub with small, sub-sessile, greenish brown leaves and small, red or rose color
(Chauhan and Pillai 2007), scented flowers (Simon et al. 1984). It tolerates extreme
heat and long droughts and grows wild in semi-arid regions (Kidanemariam et al.
2013). It is a perennial shrub native to North Africa (Vasudevan and Laddha 2003)
and it is naturalized and cultivated in other parts of Africa where it is utilized for
cultural and medicinal purposes.
The species is sometimes classified as Lawsonia reba with height up to 6 m
(Simon et al. 1984). The leaves of the plant contain a dye which is used in making
patterns on the hand and nails. The chemical compound responsible for the colour-
ing effect of the dye is contained by the leaves. It is known as Hennotannic acid or
Lawsone (2-hydroxy-1, 4-napthoquinone) (Kidanemariam et al. 2013). According
to Semwal et al. (2014), Henna can be applied to the hands and feet to prevent fun-
gal infections and to the hair to combat lice and dandruff.
Lawsone levels of Henna is determined by the soil and its moisture content. Dry,
hot, iron bearing soils produce Henna with high Lawsone levels (Kidanemariam
et al. 2013) while moist, fertile soils produce Henna with a lower level of Lawsone
(Yogisha et al. 2002). Bridal Henna nights remain a popular tradition in Northern
African countries. In Algeria, the bride’s mother-in-law paints the Henna on the
bride’s hands as a tradition. In Ethiopia (Kidanemariam et al. 2013), Sudan, Eritrea,
Nigeria (Northern Nigeria), Somalia and several other African countries, L. inermis
is traditionally used to make patterns on the hands, feet, nails and colourings on the
hair for occasions such as weddings and religious festivals. In the last fifteen years,
Henna body art has, recently, become very popular, spreading across the globe from
its areas of origin, and has changed from being just a traditional bridal and festival
adornment to an exotic fashion accessory (Semwal et al. 2014).
Henna dye is prepared by mixing a mildly acidic liquid with powdered Henna
leaves to prevent oxidation of Lawsone, and left to soak for several hours, (Pradhan
et al. 2012). The product is then applied with tools which place the wet paste in
contact with hair, nails or skin (Semwal et al. 2014). The colourant penetrates,
stains, and binds to keratin in the skin, nails and hair, without heating and the wet
paste is then scraped or rinsed off the skin (Semwal et al. 2014). A high percentage
of the Lawsone remains in the outermost, dead skin layer, while about 1% moves
into the blood-bearing, living layers and carried away by urine (Kraeling et al.
2007). Its flowers are scented and are used to extract perfume which is used as base
for local scents.
Lawsonia inermis (Henna) is also a medicinal plant (Mitscher et al. 1972;
Cajkovac et al. 1996; Singh and Singh 2001; Azaizeh et al. 2003) as its leaves are
used in the treatment of wounds, anaemia, ulcers, lumbago, rheumatagia, inflamma-
tions, bronchitis, diarrhoea, leucoderma, scabies, boils, haemorrhages, cough, fever
and dysentery (Shihata et al. 1978; Vaidyratnam 1995). It is used for alleviating
jaundice, venereal diseases, skin diseases, smallpox and spermatorrhoea (Chaudhary
et al. 2010). An infusion of the flowers, when applied to bruises can facilitate heal-
ing processes (Chaudhary et al. 2010). It is used in the treatment of liver and diges-
tive disorders, diabetic foot disorders, reduction of tissue loss in leprosy, and ulcers
(Semwal et al. 2014). Apart from Henna, there are several other valuable MP
endemic to Northern Africa.
ighly Utilized Medicinal Plants Across the Northern African

H
Countries
Several plant species endemic to Northern African countries are utilized in these
countries for similar ailments/diseases and form an integral part of their TM sys-
tems. Ephedra alata is used in Algeria, Libya, and Morocco to treat asthma, hyper-
tension, headaches and pulmonary diseases (Boulos 1983). Lepidium sativum seeds
crushed with honey is used as a treatment for cough and pulmonary affections, and
skin problems (De Natale and Pollio 2012). The leaves are effective in the preven-
tion of scurvy and are often used as a condiment (Boulos 1983; Bellakhdar et al.
1991). The milk infusion of the seeds is used to cure migraines (De Natale and
Pollio 2012). Seeds boiled in oil are used to treat diarrhea while the powdered seeds
are applied topically to treat skin ulcers and warts (De Natale and Pollio 2012).
The seeds also form part of a preparation used to treat sexual impotence (Merzouki
et al. 2000).
Globularia alypum branch and leaf decoctions are prepared for the treatment of
intermittent fevers, arthritis and rheumatisms (Boulos 1983; Bellakhdar et al. 1991).
The decoction of the aerial parts is used for constipation, fever, and mycosis
(Hammiche and Maiza 2006). Cyperus rotundus tubercles are used to cure a wide
range of infections (Boulos 1983). In Sudan, a tuber decoction is used to treat stom-
ach problems (El-Kamali and El-Khalifa 1999). Its stem galls is mixed with leaves
of Lawsonia inermis, powdered, kneaded with water and applied as a hair tonic
(Merzouki et al. 2000). In Morocco, the plant is used as a cosmetic for hair care
(Bellakhdar et al. 1991).
Balanites aegyptica leaf decoction is used for medicinal purposes in the central
Sahara (Algeria) and in other Northern African countries, as an anthelminthic and
for skin infections (De Natale and Pollio 2012). It is also used as a cure for stomach,
liver, pulmonary and spleen infections (Boulos 1983; Hammiche and Maiza 2006).
In Sudan, the fruit is used as a purgative and a bark decoction against jaundice
(El-Kamali and El-Khalifa 1999). It is also used as a purgative and for bilharzias
(Hussein and Svendsen 1981).
Marula, a Revered South African Native
Marula tree (Sclerocarya birrea) is a member of Anacardiaceae family (Pretorius

et al. 1985). It is distributed across Africa with its southern-most location in the
lowlands of KwaZulu-Natal (South Africa) and extends northwards through tropical
Africa into Ethiopia and Sudan (Van Wyk 1974). In the aspect of domestication and
commercialization, Marula has received most attention of all the fruit trees endemic
to South Africa (Shackleton 1996). It is among the most valued of Southern African’s
trees as a result of its multipurpose use and its edible fruit. There is currently an
increasing demand for S. birrea fruit in various parts of Southern Africa and beyond.
As a result of the high and increasing demand of Marula beer and nut oil the plant
have attained high economic value and serve as source of livelihood in many rural
communities within SADC. Sclerocarya birrea is highly valued for its nutritional
and financial benefits to several poor communities within SADC.
The Tonga people celebrate feast of the First Fruits by pouring drink offering of
fresh juice from the fruit on the tombs of dead chiefs (Palgrave 1983). The pulp of
the fruit is considered delicious and the large nut is also edible (Eloff 2001). Some
tribes such as the Pedi also make delicacies from the leaves (Fox and Young 1982).
Traditional Zulu healers wash themselves in the bark decoctions of Marula before
commencing treatment of patients with gangrenous rectitis and at the same time use
the decoction to treat the patient (Bryant 1966). To treat dysentery and diarrhoea in
some parts of Southern Africa, 300 ml doses of Marula bark decoction is taken
(Watt and Breyer-Brandwijk 1962).
The bark of Marula has also been used for the treatment of proctitis (Eloff 2001)
and the Vhavenda people use it to treat fevers and ulcers (Mobogo 1990). The roots
of the plant are used for many purposes including sore eyes in Zimbabwe
(Gelfand et al. 1985). In East Africa, Marula is an ingredient of an alcoholic medi-

cine used for treating an internal ailment known as kati while its bark is used for
stomach disorders (Kokwaro 1976). The Hausa people in West Africa use a cold
infusion of the bark together with native natron as a traditional remedy to treat dys-
entery (Oliver-Bever 1986).
Products such as beer, juice, jam and jelly have been developed from the meso-
carp of Marula fruit and are successfully marketed (Burger et al. 1987) within South
Africa and beyond. Ripe fruits of Marula can also be consumed by biting or cutting
through the thick leathery skin and sucking the juice or chewing the mucilaginous
flesh after the skin has been removed (Viljoen et al. 2008). The leaf and bark infu-
sions are often used to treat diarrhoea, boils and malaria while the oil from the seeds
is used to preserve meat and utilized as a cosmetic (Shone 1979).
calypha species Utilized in the African Traditional Medicine

A
Systems
The genus Acalypha belong to the family Euphobiaceae and is widespread across
Africa. About 100 species of Acalypha are known from Africa (Levin et al. 2007).
In the African TM, Acalypha species is of high relevance (Adesina et al. 2000;
Akinyemi et al. 2005; Wiart et al. 2004; Motsei et al. 2003). Acalypha manniana is
a slender climbing shrub that grows to about 6 m high in marshy places and found
in scattered locations from Rwanda, Burundi, Uganda, across northern DRC,
Cameroun, Nigeria, Benin, and Ghana (Noumedem et al. 2013) while A. guineensis
is restricted to the mountains of Upper Guinea (Levin et al. 2007).
edicinal Uses of Acalypha manniana, and A. wilkesiana

M
in Africa
Traditional healers found in the Western region of Cameroon, use decoction of the
leaves for the treatment of mycosis and various skin diseases (Noumedem et al.
2013). A leafy stem decoction of A. manniana is taken in some African countries
such as Ivory Coast, Ghana, Uganda, Rwanda, Burundi and Cameroon to treat diar-
rhoea (Schmelzer 2007; Schmelzer and Gurib-Fakim 2008). Acalypha wilkesiana is
used in West Africa for the treatment of headache and cold while the cold leaf
extract is popularly used in Nigeria to bath babies with skin infection (Adesina et al.
2000) such as eczema.
7 A
romatic Plants, an Integral Part of Human Day to Day
Life
The appeal of scented flowers and leaves and their by-products are as irresistible
today as it was in the first fragrant plot, the “Garden of Eden” (Allardice 1992). A
garden should appeal to all senses especially the sense of smell and sight (La Croix
1984). Aromatic plants have been used for innumerable purposes among which are
food and drink flavouring, scenting of clothes and to uplift the spirits of humans for
many centuries (Allardice 1992). Aromatic plants possess volatile substances in the
form of essential oils in one or more parts of the plant (Skaria et al. 2007). Many
essential oils are used commercially for the production of perfumes (La Croix
1984).
The use of fragrances for pleasure is very old; an Egyptian papyrus from around
the sixteenth century BC gives recipes for perfumes, cosmetics and deodorants (La
Croix 1984). Perfume was integral to ancient Egypt civilizations and the perfumes
were derived from aromatic plants (Allardice 1992). The Egyptians used perfumes
as an offering to their Gods and the aromatic products were prepared and kept in a
small room in their temples (Skaria et al. 2007). According to Skaria et al. (2007),
incense and aromatic oils were used in religious ceremonies and to ward off evil
spirits.
The oil of cedar wood collected from the dense forests of Mount Lebanon was in
much demand by the Egyptians who used it for religious rites, fragrant cosmetics
and ointments and they believed that cedarwood was imperishable and could pre-
serve life (Allardice 1992). The Hebrews got the knowledge about aromatic per-
fumes from their countrymen who were held as prisoners by Egyptians (Skaria et al.
2007). In Elizabethan times and earlier, floors were strewed with aromatic herbs
such as Acorus calamus, chamomile, thyme, hyssop and lavender (La Croix 1984).
For several thousands of years, the “Incense Road” between Arabia and Egypt was
travelled by the wealthy spice caravanserai and the rewards for a successful incense
merchant were great and the entire economy of the kingdom of Sheba in the south-
ern part of Africa depended on the sale of Frankincense and Myrrh (Allardice 1992).
In the Middle East, Egypt is one of the most important producers of aromatic and
medicinal crops (Elsabawy 2012). The Egyptians also had knowledge about the
efficacy of aromatic plants in healing diseases and this pool of knowledge was
applied in medical care (Skaria et al. 2007).
When plague was a fact of life, lice and fleas were man’s constant companions
and waste was disposed through the nearest open window, scented flowers were
used for their antiseptic qualities and also to mask the odour (Verey 1981). In an
attempt to stop the spread of plague in Athens, Hippocrates fumigated streets with
aromatic plants (Skaria et al. 2007). Herbs were grown and utilized not only for
their scents but also for their medicinal uses (La Croix 1984) and some of them have
remarkable application as therapeutic agents in pharmaceutical and drug industries
(Skaria et al. 2007). A rhythm exhibiting calmness is produced when essential oil
with a sedative effect is inhaled as it stimulates the sense of smell and the emotional
centre of the brain (Skaria et al. 2007). In light of this, aromatherapy has become a
lucrative business and the production and export of essential oil from plants is on
the increase in Africa.
8 N
orthern Africa at the Forefront of Aromatic Plant
Production and Export
“The breeze of essential oils of the world has blown from yesterday’s narrow defini-
tion as a symbol of luxury to indispensable necessities for both the rich and poor
from the cradle of infancy to the silence of the grave” (Skaria et al. 2007). In ancient
Egypt, women used cinnamon oil (from Nepal) combined with honey, myrrh and
almonds to make a perfume for the feet and legs (Allardice 1992). At present, Egypt
is one of the leading African exporters of aromatic plant material to the international
market (Tsey 1997). In Morocco, sale of aromatic MP is a very lucrative business as
a result of which 14,468 tons of essential oils and various plant material worth Dh
300,000 is exported annually (Kouhila et al. 2002). The highly utilized plants are
Rosmarinus officinalis, from which 60 tons of essential oil are extracted and
exported, Thyme and Lavender species, Artemisia herba-alba, Mentha pulgemium,
Origanum compactum and Coriander sativum (Montanari 2004).
In Sudan, an incense mixture called bakour, composed of gum, exudates, balsam
and dried plant material which is burnt in a traditional clay incense burner to give a
fragrant smoke believed to repel insects, clean air and make the environment smell
pleasant (Khalid et al. 2012). In Sudan, there is a unique tradition of preparing sev-
eral different local perfumes of which the basic compounds are Khumra, which is
prepared from the seeds of Prunus mahleb and Santalum album powder (Khalid
et al. 2012). Khumra can be classified as Khumra zeit which is oil-based, Khumra
dufra includes shells and Khumra misk contains extracts of crocodile sweat glands
(Khalid et al. 2012).
In Algeria and Tunisia, Thymus algeriensis is used as a culinary herb (Chaieb and
Boukhriss 1998). Thymus algeriensis which is endemic to Lybia, Tunisia, Morocco
and Algeria (Houmani et al. 2002), is an herbaceous fragrant plant largely used,
fresh or dried, as a culinary herb (Chaieb and Boukhriss 1998). This plant is widely
used in TM against illnesses of the digestive tube (Le Floch 1983). Globally,
Morocco is the ninth largest exporter of medicinal and aromatic plants (Ozhatay
et al. 1997). Egypt and Morocco are among two major countries from Africa
involved in the export of essential oil into the European Union (EU) (Montanari
2004). In SADC, South Africa is one of the leading countries involved in the growth,
sale and export of indigenous aromatic plants and their products. The Rutaceae fam-
ily which has a high percentage of species endemic to South Africa is among the
family with species which their essential oil and products are marketed locally.
9 B
uchu, a Genus of Dynamic Aromatic Plants of the South
African Flora
The family Rutaceae to which citrus belong to are easily identified on the field as a
result of dotted oil glands on the reverse side of their leaves. As a result of this and
scents associated with species of the Rutaceae, several species are utilized in the
production of perfumes, flavors and spices. In South Africa, members of the
Rutaceae, most especially species of the genus Agathosma are referred to as Buchu.
‘Buchu’ (Agathosma species) has been an indispensable part of the San and Khoi
traditional healing culture in the Cape and is still being used for this purpose
throughout South Africa (Moolla et al. 2007).
The San people used aromatic plants lubricated with fat to keep their skin soft
and moist in the desert climate, and as an antifungal agent to promote good health
through the uptake of aromatic substances by the skin (Simpson 1998). Agathosma
ovata is still used for medicinal purposes amongst the Cape people (Van Wyk and
Gericke 2000). The South African indigenous plant, Agathosma betulina, is among
the most popularly used medicinal plant in the family Rutaceae in the South African
TM system. This has resulted in the local cultivation of these plants by farmers,
indigenous nurseries and National conservation bodies.
Essential oil of Agathosma betulina and other Agathosma species are marketed
by several local and international natural product companies. Buchu, Agathosma
betulina oil is used for medicinal and cosmetic purposes (Van Wyk et al. 1997).
Globally, Buchu is highly sought after for its essential oil which has a high diosphe-
nol content (Moolla et al. 2007). Moolla and Viljoen (2008) reported that a kilo-
gramme of A. betulina oil sells on the international market for about 700 Euro.
Irrespective of the vast amount of medicinal and aromatic plants Africa is blessed
with and uniqueness of its TM system, the future of Africa’s TM is dependent on its
integration into the healthcare system of African countries and the formulations of
policies which will encourage its growth. Moreover, its sustainability is also depen-
dent on the incorporation of health policies which can support the growth and docu-
mentation of TM and MP used for specific health conditions.
10 A
Transition in the Acceptance of African Traditional
Medicine from the Days of the Colonial Masters
to the Present Age
In Africa, during colonisation; colonial governments connected TM Practitioners

(TMPs) to the use of witchcraft, and TM was discredited and placed on legal bans
(Stangeland et al. 2008). TM was outlawed (Bodeker 1994; Obbo 1996; Svarstad
and Dhillion 2000) and in several African countries, modern medicine was officially
recognised while TM and its practitioners were not tolerated (Tabuti et al. 2003).
When colonization ended, independence brought about a more tolerant disposition
towards TM, which helped to regain African identity and cultural values (Stangeland
et al. 2008). The passion of African leaders and world health organization (WHO)
towards the development of TM cannot be underestimated (Kasilo et al. 2010). This
is evident by the declarations made at African Summits of Head of States and
Government such as the declaration of 2001–2010 as the Decade for African TM,
and the institution of African TM Day for Advocacy by WHO (Kasilo et al. 2010).
Throughout the Third World, TM is being promoted by WHO and other interna-
tional agencies (WHO 1978; Leslie 1980; Jingfeng 1987; Launs 1989; Chavunduka
1994). The Inter-African Experts Committee on African TM and MP in the
Organization of African Unity (OAU) led to proclamation of the Decade of African
Medicine from 2001 to 2010 (Mahunnah 2002), while the African TM Day was set
to be on the 1st of September (Stangeland et al. 2008). The facilitation, recognition,
acceptance, development and institutionalization of TM are part of the key priorities
of the African Union action plan (Kasilo et al. 2010).
11 Progress Made so far by African Countries
As at 2010, few countries have developed national policies, legal frameworks, and
codes of ethics regulating the practice of TM (WHO Regional Office for Africa
2010). Diverse forces are responsible for the integration of services of Traditional
Health Practitioners (THPs), they include the cultural acceptance of TM, which
majority of African population use to cater for their primary health care needs
(Kasilo et al. 2010). Allopathic health care providers in some African countries such
as Ghana, Senegal, Burkina Faso, Kenya, Uganda, South Africa, Tanzania and
Zimbabwe have been collaborating with THPs in the use of TM and HIV/AIDS
prevention activities. Researchers have observed a reduction in viral load, an
improvement in the clinical condition of HIV/AIDS patients, and in some cases,
weight increase (Kasilo et al. 2010).
A number of countries have made good progress in the area of TM policy formu-
lation but some of the policies are yet to be translated into implementation (Kasilo
et al. 2010). In 2010, 39 of the 46 African countries already had established national
offices for TM (Kasilo et al. 2010). According to an AU report (2007), Benin
Republic, Burkina Faso, Cameroon, Cote d Ivoire, Equatorial Guinea, Ethiopia,
Kenya, Ghana, Guinea, Madagascar, Malawi, Mali, Mozambique, Nigeria, Senegal,
South Africa, Tanzania, Togo, Uganda, Zambia and Zimbabwe have enacted some
laws and regulations to govern TM while Angola, Central African Republic, Chad,
DRC, Equatorial Guinea, Gambia, Rwanda, Sao Tome and Principe, and Seychelles
are developing laws and regulations.
TM products form a crucial part of the healthcare system of SADC countries and
there is a cooperation project to establish a regional databank of MP and develop
policies and legal frameworks for its practice within the region (Lezotre 2014). The
progress made so far coupled with support from African leaders could make African
TM accepted globally just like for Chinese TM.
Acknowledgement O.O. Fajinmi gratefully acknowledges J. Van Staden for his mentorship over
the years.
References
Adebayo SA, Oladele OI (2012) Medicinal values of Kolanut in Nigeria: implication for extension
service delivery. Life Sci J 9:887–891
Adesina SK, Idowu O, Ogundaini AO, Oladimeji H, Olugbade TA, Onawunmi GO, Pais M (2000)
Antimicrobial constituents of the leaves of Acalypha wilkesiana and Acalypha hispida.
Phytother Res 14:371–374
Akinyemi KO, Oladapo O, Okwara CE, Ibe CC, Fasure KA (2005) Screening of crude extracts of
six medicinal plants used in South-West Nigerian unorthodox medicine for anti-methicillin
resistant Staphylococcus aureus activity. BMC Complement Altern Med 5:6–10
Allardice P (1992) The scented garden. A complete guide to growing and using fragrant plants.
Collins Angus and Robertson Publishers, Australia.
Asogwa EU, Anikwe JC, Mokwunye FC (2006) Kola production and utilization for economic
development. Afr Sci 7:217–222
Azaizeh H, Fulder S, Khalil K, Said O (2003) Ethnobotanical knowledge of local Arab practitio-
ners in the Middle Eastern region. Fitoterapia 74:98–108
Bellakhdar J, Claisse R, Fleurentin J, Younos C (1991) Repertory of standard herbal drugs in the
Moroccan pharmacopoea. J Ethnopharmacol 35:123–143
Bodeker G (1994) Traditional health knowledge and public policy. Nat Res Forum 30:5–16
Boulos L (1983) Medicinal plants of North Africa. Algonac, Michigan
Bryant AT (1966) Zulu medicine and medicine men. Struik, South Africa
Burger AEC, De Villiers JBM, Du Plessis LM (1987) Composition of the kernel oil and protein of
the marula seed. S Afr J Sci 83:733–735
Burkill HM (1985) The useful plants of West Tropical Africa, 2nd edn. Pergamon Press, Richmond
Cajkovac M, Oremovic L, Cajkovac V (1996) The effect of hair colours and perm products on the
state of hair. Acta Pharma 46:39–49
Chaieb M, Boukhriss M (1998) Flore suscinte et illustrée des zones arides et sahariennes de
Tunisie. Association de la Protection de la Nature et de l’Environnement, l’Or du Temps (ed),
Sfax, Tunisia, 42
Chaudhary G, Goyal S, Poonia P (2010) Lawsonia inermis Linnaeus: a phytopharmacological
review. Int J Pharm Sci Drug 2:91–98
Chauhan MG, Pillai AP (2007) Microscopic profile of powdered drug used in Indian system of
medicine, vol II. Gujarat Ayurved University, Jamnagar, India
Chavunduka GL (1994) Traditional Medicine in modern Zimbabwe. University of Zimbabwe
Publications, Zimbabwe.
De Natale A, Pollio A (2012) A forgotten collection: the Libyan ethnobotanical exhibits (1912–14)
by A. Trotter at the Museum O. Comes at the University Federico II in Naples, Italy. J Ethnobiol
Ethnomed 8:1
Doherty VF, Olaniran OO, Kanife UC (2010) Antimicrobial activities of Aframomum melegueta
(Alligator pepper). Int J Biol 2:126
El-Kamali HH, El-Khalifa KF (1999) Folk medicinal plants of riverside forests of the Southern
Blue Nile district, Sudan. Fitoterapia 70:493–497
Eloff JN (2001) Antibacterial activity of Marula (Sclerocarya birrea (A. rich.) Hochst. subsp. caf-
fra (Sond.) Kokwaro) (Anacardiaceae) bark and leaves. J Ethnopharmacol 76:305–308
Elsabawy MNE (2012) Medicinal and aromatic crops in Egypt: a study in medical geography.
ARPN J Sci Technol 2:207–221
Esiegwu AC, Okoli AC, Emenalom OO, Esonu BO, Udedibie ABI (2014) The emerging nutricitical
benefits of the african wonder nut (Garcinia Kola Heckel): a review. Glob J Anim Sci Res 2:2
Fox FW, Young MEN (1982) Food from the veld. Edible wild plants of Southern Africa. Delta
Books, Johannesburg
Galal AM (1996) Antimicrobial activity of 6-paradol and related compounds. Int J Pharmacogn
34:64–69
Gelfand M, Mavi S, Drummond RB, Ndemera B (1985) The traditional medical practitioner in
Zimbabwe. Mambo Press, Gweru
Hammiche V, Maiza K (2006) Traditional medicine in Central Sahara: pharmacopoeia of Tassili
N’ajjer. J Ethnopharmacol 105:358–367
Houmani Z, Azzoudj S, Naxakis G, Skoula M (2002) The Essential Oil Composition of Algerian
Zaâtar: Origanum spp. and Thymus spp. J Herbs Spices Med Plants 9:275–280
Hussein SM, Svendsen AB (1981) Medicinal and aromatic plants in the Sudan: usage and explora-
tion. Fitoterapia 52:243–246
Ibiblio JO (1983) Some medicinal plants of Nigeria. Ibadan publishers Nigeria Limited, Nigeria,
pp 132–133
Iwu MM (2014) Handbook of African medicinal plants. CRC Press/Taylor and Francis Group,
Boca Raton/London
Jingfeng C (1987) Towards a comprehensive evaluation of alternative medicine. Soc Sci Med
25:659–667
Kasilo MJO, Trapsida JM, Mwikisa CN, Lusamba-Dikassa PS (2010) An overview of the
traditional medicine situation in the African region (Decade of African Traditional Medicine,
2001–2010). African Health Moni Spec Issue 14
Khalid H, Abdalla WE, Abdelgadir H, Opatz T, Efferth T (2012) Gems from traditional north-
African medicine: medicinal and aromatic plants from Sudan. Nat Prod Bioprospect
2:92–103
Kidanemariam TK, Tesema TK, Asressu KH, Boru AD (2013) Chemical investigation of Lawsonia
inermis L. leaves from Afar region, Ethiopia. Orient J Chem 29:1129–1134
Kokwaro JO (1976) Medicinal plants of East Africa. East African Literature Bureau, Nairobi
Kouhila M, Kechaou N, Otmani M, Fliyou M, Lahsasni S (2002) Experimental study of sorption
isotherms and drying kinetics of Moroccan Eucalyptus globulus. Dry Technol 20:2027–2039
Kraeling ME, Bronaugh RL, Jung CT (2007) Absorption of lawsone through human skin. Cutan
Ocul Toxicol 26:45–56
La Croix IF (1984) Scented plants in Southern Africa. Macmillan South African, Johannesburg
Launs L (1989) Integrated medicine-a challenge to the health-care system. Acta Sociologica
32:237–251
Le Floch E (1983) Contribution à une étude ethnobotanique de la flore tunisienne. Ministère de
l’Enseignement Supérieur et de la Recherche Scientifique (ed), Imprimerie Officielle de la
République Tunisienne, 402
Leakey RRB (1999) Potential for novel food products from agroforestry trees: a review. Food
Chem 66:1–14
Leslie C (1980) Medical pluralism in world perspective (1). Soc Sci Med Part B Med Anthropol
14:191–195
Levin GA, Morton JK, Robbrecht E (2007) Two new species of Acalypha (Euphorbiaceae) from
Tropical Africa, and a review of some Robyns names for cupricolous plants from the Democratic
Republic of the Congo. Syst Bot 32:576–582
Lewis R (2001) Discovery: science as a window to the world. Black Science Property Inc, USA
Lezotre PL (2014) International cooperation, convergence and harmonization of pharmaceutical
regulations: a global perspective. Academic, Oxford
Mahomoodally MF (2013) Traditional medicines in Africa: an appraisal of ten potent African
medicinal plants. Evid Based Complement Alternat Med 2013:1–14
Mahunnah RLA (2002) Ethnobotany and conservation of medicinal plants in Africa: the way for-
ward in the next decade. In: Proceedings of the 15th meeting of the Inter-African Experts
Committee on African Traditional Medicine and Medicinal Plant, OAU Lagos, Nigeria
Merzouki A, Ed-Derfoufi F, Mesa JM (2000) Contribution to the knowledge of Rifian traditional

medicine. II: Folk medicine in Ksar Lakbir District (NW Morocco). Fitoterapia 71:278–307
Mitscher LA, Leu RP, Bathala MS, Wu WN, Beal JL, White R (1972) Antimicrobial agents from
higher plants. I: Introduction, rational, and methodology. Lloydia 35:157–166
Mobogo DEN (1990) Unpublished M.Sc., thesis, University of Pretoria
Montanari B (2004) Alternative paths to commercialising medicinal and aromatic plants in a com-
munity of the High Atlas Mountains, Morocco. University of Kent, Canterbury
Moolla A, Viljoen AM (2008) ‘Buchu’-Agathosma betulina and Agathosma crenulata (Rutaceae):
a review. J Ethnopharmacol 119:413–419
Moolla ASF, Van Vuuren RL, Van ZYL, Viljoen AM (2007) Biological activity and toxicity profile
of 17 Agathosma (Rutaceae) species. S Afr J Bot 73:588–592
Motsei ML, Lindsey KL, Van Staden J, Jäger AK (2003) Screening of traditionally used South
African plants for antifungal activity against Candida albicans. J Ethnopharmacol
86:235–241
Noumedem JA, de Dieu TJ, Teke GN, Momo RC, Kuete V, Kuiate JR (2013) Phytochemical analy-
sis, antimicrobial and radical-scavenging properties of Acalypha manniana leaves. Springerplus
2:1–6
Obbo C (1996) Healing: cultural fundamentalism and syncreticism in Buganda. Africa
66(2):183–201
Oliver-Bever BEP (1986) Medicinal plants in tropical West Africa. Cambridge University Press,
Cambridge
Omode AA, Fatoki OS, Olagun KA (1995) Physico-chemical properties of some underexploited
and unconventional oilseeds. J Agric Food Chem 43:2850–2853
Ozhatay N, Koyuncu M, Atay S, Byfield AJ (1997) The wild medicinal plant trade in Turkey.
DHKD/WWWF. TRAFFIC, Istanbul
Palgrave KC (1983) Trees of Southern Africa. C. Struik, Cape Town
Pradhan R, Dandawate P, Vyas A, Padhye S, Biersack B, Schobert R, Ahmad A, Sarkar F (2012)
From body art to anticancer activities: perspectives on medicinal properties of henna. Curr
Drug Target 13:1777–1798
Pretorius V, Rohmer E, Rapp A, Holtzhausen LC, Mandery H (1985) Volatile flavour components
of marula juice. Zeitschrift für Lebensmitteluntersuchung and Forschung 181:458–461
Schmelzer GH (2007) Acalypha ornata Hochst. ex A.Rich. In: Schmelzer GH, Gurib-Fakim A
(eds) Medicinal plants/Plantes médicinales. Prota, Wageningen
Schmelzer GH, Gurib-Fakim A (2008) Ressources végétales de l’Afrique tropicale 11. Plantes
Médicinales 1:869
Semwal RB, Semwal DK, Combrinck S, Cartwright-Jones C, Viljoen A (2014) Lawsonia inermis
L. (henna): Ethnobotanical, phytochemical and pharmacological aspects. J Ethnopharmacol
155:80–103
Shackleton CM (1996) Potential stimulation of local rural economies by harvesting secondary
products: a case study of the central Transvaal Lowveld, South Africa. Ambio 25:33–38
Shihata IM, Hassan AG, Mayah GY (1978) Pharmacological effects of Lawsonia inermis leaves
(el-henna). Egypt J Vet Sci 15:31
Shone AK (1979) Notes on the marula. Bulletin 58, Department of Forestry, Pretoria, South Africa
Shumba E, Carlson A, Kojwang H, Sibanda M, Masuka M, Moyo N (2009) Traditional medicinal
plant practice in Southern Africa: a situation analysis in Zambia and Zimbabwe. WWF-World
Wide Fund for Nature (Formerly World Wildlife Fund) Harare, Zimbabwe
Simon JE, Cahadwick AF, Craker LE (1984) Herb: an indexed bibliography, 1971–1980. The sci-
entific literature on selected herbs, aromatic and medicinal plants of the temperature zone.
Archon Books Press, Purdue University, Hamden
Simpson D (1998) Buchu – South Africa’s amazing herbal remedy. Scott Med J 43:189–191
Singh A, Singh DK (2001) Molluscicidal activity of Lawsonia inermis and its binary and tertiary
combinations with other plant derived molluscicides. Indian J Exp Biol 39:263–268
Skaria BP, Joy PP, Matthew S, Mathew G, Joseph A, Joseph R (2007) Aromatic plants. New India
Publishing Agency, New Delhi
Stangeland T, Dhillion SS, Reksten H (2008) Recognition and development of traditional medicine
in Tanzania. J Ethnopharmacol 117:290–299
Svarstad H, Dhillion SS (2000) Responding to bioprospecting: from biodiversity in the south to
medicines in the north. Spartacus Forlag, Oslo
Tabuti JRS, Dhillion SS, Lye KA (2003) Traditional medicine in Bulamogi county, Uganda: its
practitioners, users and viability. J Ethnopharmacol 85:119–129
Tsey K (1997) Traditional medicine in contemporary Ghana: a public policy analysis. Soc Sci Med
45:1065–1074
Vaidyratnam PS (1995) Indian medicinal plant; a compendium of 500 Species, vol III. Botanical
Survey of India, Ministry of Environment and Forests, India, New Delhi, pp 303–304
Van Wyk P (1974) Sclerocarya caffra trees of the Kruger National Park. Purnell, Cape Town
Van Wyk BE, Gericke N (2000) People’s plants: a guide to useful plants of Southern Africa. Briza
Van Wyk BE, Van Quadshoom B, Gericke N (1997) Medicinal plants of South Africa. Briza
Publication, Pretoria, p 236
Vasudevan TN, Laddha KS (2003) Herbal drug microscopy. Yucca Publishing House, New Delhi
Verey R (1981) The scented garden; choosing, growing and using the plants that brings fragrance
to your life, home and table. Michael Joseph Limited, London
Viljoen AM, Kamatou GPP, Başer KHC (2008) Head-space volatiles of marula (Sclerocarya
birrea subsp. caffra). S Afr J Bot 74:325–326
Watt JM, Breyer-Brandwijk MG (1962) The medicinal and poisonous plants of southern and east-
ern Africa. E.S. Livingstone, Edinburgh
WHO Regional Office for Africa (2010) Decade of African TM (2001-2010). African Health
Monit Spec Issue 14:4–103
Wiart C, Hannah A, Yassim M, Hamimah H, Sulaiman M (2004) Antimicrobial activity of
Acalypha siamensis Oliv. ex Gage. J Ethnopharmacol 95:285–286
World Health Organization (1978) The promotion and development of traditional medicine: report
of a WHO meeting (held in Geneva from 28 November to 2 December 1977)
Yogisha S, Samiulla DS, Prashanth D, Padmaja R, Amit A (2002) Trypsin inhibitory activity of
Lawsonia inermis. Fitoterapia 73:690–691
Medicinal and Aromatic Plants
of the World – Africa
Ossy M.J. Kasilo, Mawuli Kofi-Tsekpo, and Francis Gachathi
Abstract The medicinal and aromatic plants of Africa are found in the continent
from Cape Town to Cairo and have been used in the African people’s traditional
culture of health for centuries. The declaration of Alma Ata by the International
Conference on Primary Health Care in 1978 was a significant turning point in the
development of health systems in the developing countries in Africa. This declara-
tion provided for incorporation of traditional medicine in health systems. Medicinal
plants and aromatic plants form the major component of African traditional medi-
cine practice and these have become very important in the development of health
systems in Africa.
Therefore, in 2001 the Regional Committee of the WHO African Region adopted
a regional strategy on promoting the role of traditional medicine in health systems
(WHO, Promoting the role of traditional medicine in health systems: a strategy for
the African Region (Strategy documents, AFR/RC50/9 and Resolution AFR/RC65/
R3). WHO Regional Office for Africa, Harare, Zimbabwe, 2001). In 2013 the
Regional Committee further emphasized the enhancement of the role of traditional
medicine in health systems in the African Region (WHO, Enhancing the role of
traditional medicine in health systems: a strategy for the African Region (Strategy
documents, AFR/RC63/6 and Resolution AFR/RC65/R3). WHO Regional Office
for Africa, Brazzaville, Republic of the Congo, 2013). If medicinal and aromatic
plants are to be incorporated in health systems then, their identity and quality must
be assured in order to establish the safety and efficacy of the medicines they are used
to manufacture.
O.M.J. Kasilo (*)

Health Technologies and Innovation (HTI), Health Systems and Services Cluster,
World Health Organization, Regional Office for Africa, P.O. Box 6, Brazzaville,
Republic of the Congo
M. Kofi-Tsekpo
Consolata Hospital, P.O. Box 2005, 60200 Nkubu, Meru, Kenya
F. Gachathi
Kenya Forest Research Institute, P.O. Box 200412, 00200 Nairobi, Kenya

DOI 10.1007/978-94-024-1120-1_4
78 O.M.J. Kasilo et al.
This chapter contains monographs on 15 medicinal plants which are of major

health and commercial importance in the world today. Each monograph provides
the following information: Botanical name, Synonyms, Geographical Distribution,
Description, Parts used for medicine preparation, Chemical constituents identified,
Pharmacology and therapeutics and References.
Keywords Africa • Traditional medicine • Monographs • Pharmacology
1 Introduction
Medicinal and aromatic plants have, for centuries, constituted an inseparable aspect
of the African traditional culture of health. They are part of the nutritive, preventive,
curative, and spiritual components of the African way of life and the African tradi-
tional health systems. The documentation of these concepts of the African health
systems have been given through oral traditions for centuries. However, it was only
in the last century that some written accounts began to appear and such notable
publications include those of Watt and Breyer-Brandwijk (1962), Iwu (1993),
Kokwaro (2009) and Gachathi (2007).
Information on African Health Systems The information on the African health sys-
tems particularly, the one involving medicinal and aromatic plants has remained in
the secret domain through oral communication up to now. This has been partly due
to the abolition of traditional medicine during the colonial period and also due to the
secret taboos associated with the practice of African traditional medicine. The need
for some openness arose when the World Health Organization proposed the incor-
poration of traditional medicine in health systems.
2 T
he Role of Medicinal and Aromatic Plants in Health
Systems in Africa
The declaration of Alma Ata in 1978 jointly sponsored by the World Health
Organization (WHO) and the United Nations International Children’s Fund
(UNICEF) as decided by the World Health Assembly (WHO resolutions) brought
into focus the practice of African traditional medicine and, by default, the signifi-
cance of medicinal and aromatic plants on the continent. The declaration provided
for the use or incorporation of African traditional medicine in the western-style
health systems in order to achieve a greater health care coverage in the communities
on the continent. However, this brought out some logistical challenges. The WHO
Regional Office for Africa took the challenge to rationalize the incorporation of
traditional medicine in health systems. This would require the establishment of
Medicinal and Aromatic Plants of the World – Africa 79
quality standards for: (a) the mode of practice of traditional medicine, (b) the
authentication and quality of the practitioners, and (c) the medicinal and aromatic
plants used for treating patients. In this respect the WHO has been assisting the
member states in the African region to carry out these rationalizations. The materi-
als from medicinal and aromatic plants are critical elements for the rationalization
of the use of the culture-based practices in contemporary evidence-based health
systems.
3 Monographs, Pharmacopoeias, and Formularies
Monographs and Pharmacopoeias
Most of the African cultures have a verbal tradition and, therefore, written informa-
tion on cultural features in the past are not so readily available from Africa as from
many other parts of the world. The ethnobotanical information on the uses of these
plants was sometimes documented on herbarium labels and, in this way, ethnobo-
tanical information on a number of medicinal and aromatic plants began to accumu-
late. Systematic accounts in written form dealing with medicinal and aromatic
plants in Africa are of a fairly recent date, while reports dealing with ethnopharma-
cological aspects are more recent.
The Need for the Creation of Formularies
It is becoming evident that formularies are required for the quality control of the
preparations in African traditional medicine. The herbal preparations are almost
invariably formulated from more than two or more materials from different plant
materials. This practice has pharmaceutical, pharmacological and pharmacodynam-
ics implications. The creation of official formularies are desirable from the view-
point of drug interactions, and also in order to reduce the confounding factors that
bedevil the western-trained physician who has to reconcile his diagnosis and treat-
ment with a herbal preparation with little available information.
4 Some Selected Medicinal Plant Monographs
The monographs of the following 15 medicinal and aromatic plants are described
below as examples of the uniqueness and richness of the African flora.
Catha edulis Forssk,
Family, CELASTRACEAE
Synonyms Catha forsskalii, Catha inermis, Celastrus edulis, Celastrus tsaad,
Dillonia abyssinica, Methyscophyllum glaucum. Some common names include:
Miraa (Kenya); qat, kat (Ethiopia) and Bushman’s tea (South Africa); and the dried
leaves are also known as Abyssinian tea and Arabian tea (WHO 1980, 2006).
Geographical Distribution Catha edulis is a flowering plant which is native to the
horn of Africa and the Arabian peninsula, namely, Ethiopia, Somalia, Yemen, South
Sudan, Djibouti, Eritrea, Uganda and Kenya. Khat is also scattered in KwaZulu-
Natal and Eastern Cape, Western Cape and Mpumalanga in South Africa as well as
Swaziland and Mozambique. It has now been cultivated in other countries in Africa
(WHO 1980, 2006).
Habitat and Ecology Catha edulis is an evergreen hardy tree which normally
grows on mountain slopes or hill sides. It has been cultivated in a variety of climates
and soils and accordingly yielding wide varieties (WHO 1980, 2006). Catha edulis
grows often as a shrub but it can grow to over ten meters in a fertile mountainous
environment at altitudes of 4000–5000 m
Chemical Constituents Over 40 alkaloids have been identified in the leaves of
which two are of biological importance, namely, cathinone and cathine. The leaves
also contain other alkaloids that include kathedulins, flavonoids, glycosides, and
terpenoids (WHO 1980, 2006; Wabel 2011).
Uses in Traditional Medicine That is a traditional ritual plant of which the fresh
young shoots and leaves are chewed. The dry leaves are also smoked (WHO 1980,
2006; Wabel 2011). The leaves are also brewed as tea.
Biological Activities The chewing of khat in order to experience its stimulant
effects occurs over a short period because cathinone and cathine are readily metabo-
lized into nonstimulant derivaties. Cathinone is enolisable to the enol which is eas-
ily metabolized by the cytochrome P450 superfamily. The enolic form is then
reduced to cathine which metabolized. This explains why the users of khat have to
chew the material continuously in order to maintain the stimulant effect. The World
Health Organization therefore does not consider khat as an addictive drug.
It would be of interest to note that synthetic cathinones (which should be called
“cathinoids”) cannot undergo enolization and they therefore produce persistent hal-
lucinogenic effects.
Catharanthus roseus (L.) G. Don
Family, APOCYNACEAE
Synonyms Vinca rosea; Madagascar periwinkle; Rose periwinkle.

Geographical Distribution This is an ornamental herb which grows everywhere
in Africa. It is particularly endemic in Madagascar where is cultivated as a commer-
cial crop. It grows in the tropical and sub-tropical belt of the world.
Habitat and Ecology An evergreen, herbaceous, ornamental plant growing up to
1.5 m. There are varieties of this plant with different colored flowers: white, rose-
pink, and in different shades.
Chemical Constituents The leaves and flowers have been reported to contain
indole alkaloids, often called the vinca alkaloids, which include vinblastine and
vincristine (Van der Heijden et al. 2004; Sain and Sharma 2013).
Uses in Traditional Medicine All parts of the plant have been used in regional
herbal medicine, including the dried root, leaves, flowers and stalks. Alkaloids used
in modern medicine are extracted from the whole dried plant (Van der Heijden et al.
2004; Sain and Sharma 2013; Tiong et al. 2013; Gajalakshmi et al. 2013).
Biological Activities The vinca alkaloids vinblastine and vincristine are useful
drugs in cancer chemotherapeutics, particularly in the treatment of leukemia (Van
der Heijden et al. 2004; Sain and Sharma 2013). The traditional use of the hot water
extract for the management of diabetes mellitus has also been documented (Van der
Heijden et al. 2004; Sain and Sharma 2013).
Corynanthe pachyceras K. Schum.
Family, RUBIACEAE
Synonyms Pausinystalia johimbe, Corynanthe yohimbe; Corynanthe johimbe K.
Schum; Pausinystalia trillesii Beille; Pausinystalia yohimbe Beille.
Geographical Distribution The plant occurs in West and Central Africa:
Cameroon, Congo, Democratic Republic of the Congo, Equatorial Guinea, Gabon,
Ghana, Liberia and Nigeria
Habitat and Ecology The tree is an evergreen tree which grows from 9 to 30 m
high.
Chemical Constituents The bark is a natural source of the alkaloid yohimbine
(Putheti and Okigbo 2008; EFSA 2009; Burkill 2010)
Uses in Traditional Medicine The bark extract is traditionally used for the treat-
ment of erectile dysfunction (Putheti and Okigbo 2008)
Biological Activities Yohimbine is an alkaloid used for treatment of male impo-
tence (EFSA 2009).
Erythrina abyssinica Lam ex DC,
Family, LEGUMINOSAE
Synonyms Red hot poker tree; Lucky bean tree, Erythrina tomentosa R.Br. ex
A. Rich.
Geographical Distribution The plant is common tree on the African continent.
Habitat and Ecology It is a medium-sized tree of height between 5 and 15 m.
Chemical Constituents The bark extract contains flavonoids and iso-flavonoids.
Uses in Traditional Medicine Antioxidant and antimalarial properties against
Plasmodium falciparum (crude extract of the root bark); antiplasmodial activity
including activity against chloroquine-resistant strains of Plasmodium falciparum
(stem bark extracts) and a curare-like poison (seeds) (Burkill 2010; Yenesew et al.
2009; Kebenei et al. 2011; PROTA 2015)
Biological Activities The bark is most commonly used in traditional medicine, to
treat snakebites, malaria, sexually transmittable diseases such as syphilis and gonor-
rhoea, amoebiasis, cough, liver inflammation, stomach-ache, colic and measles.
Roasted and powdered bark is applied to burns, ulcers and swellings. The liquid
from crushed bark of green stems is used to cure conjunctivitis caused by Chlamydia
trachomatis (trachoma), whereas bark sap is also drunk as an anthelmintic. The bark
is also applied against vomiting. Pounded flowers serve to treat dysentery. A mac-
eration of the flower is drunk as an abortifacient, and applied externally to treat
earache. Roots are taken to treat peptic ulcers, epilepsy, malaria, blennorrhagia and
schistosomiasis. Leaves are taken to treat peptic ulcers; they are also used for treat-
ment of diarrhoea. A leaf decoction serves as an emetic. Leaves are applied exter-
nally to wounds and painful joints. Fruit extracts are taken to treat asthma and
meningitis (Yenesew et al. 2009).
Gymnema sylvestre R. Br,
Family, ASCLEPIADACEAE
Synonyms Asclepias geminate, Gymnema melicida, Periploca sylvestris.
Geographical Distribution A wide distribution on the African continent.
Habitat and Ecology A woody climbing plant.
Chemical Constituents The main constituents in the leaves are oleanane-type tri-
terpene saponins called gymnemic acids. Other constituents include anthraquinones
and flavonoids (Najafi and Deokule 2011; Anjum and Hasan 2013).
Uses in Traditional Medicine The extracts of G. sylvestre have been used for
centuries in the management of diabetes mellitus, although scientific proof has not
been adduced (Najafi and Deokule 2011; Anjum and Hasan 2013).
Biological Activities Antidiabetic activities have of the extracts have been demon-
strated in humans and laboratory animals (Anjum and Hasan 2013).
Hypoxis hemerocallidea, Fisch, CA Mey & Ave-Lall.
Family, HYPOXIDACEAE
Synonyms Hypoxis rooperi, African potato.
Geographical Distribution Southern Africa: Botswana, Lesotho, Mozambique,
Namibia, South Africa, Swaziland and Zimbabwe.
Habitat and Ecology This is a perennial geophyte with a tuberous rootstock.
Chemical Constituents Beta-Sitosterol, Ergosterol, Stigmasterol, Hypoxoside
(Boukes et al. 2008; Mkhize et al. 2013).
Uses in Traditional Medicine Antineoplastic properties, build up the immune sys-
tem of patients suffering from cancer and HIV and is effective in the treatment of
inflammations and the urinary system (Boukes et al. 2008; Mkhize et al. 2013).
Biological Activities The extract of the tuber has been reported to be used in the
treatment of various forms of cancer and for treatment of AIDS.
Kigelia africana (Lam.) Benth,
Family, BIGNONIACEAE
Synonyms Kigelia pinnata (Jacq.) DC, Sausage tree.
Geographical Distribution This plant is widespread across the African
continent.
Habitat and Ecology Kigelia Africana is a flowering evergreen tree growing over
20 m tall. Its fruits are cylindrical, sausage-like, about 30–60 cm long, hence its
common name.
Chemical Constituents The following groups of chemical compounds have so far
been identified: naphthaquinones, iridoids, monoterpenoids, isocoumarins, lignans,
sterols, and flavonoids (Gabriel and Olubunmi 2009; Agyare et al. 2013).
Uses in Traditional Medicine The leaves, the fruits, the stem bark, and the roots
are all used in African traditional medicine for a variety of conditions
Biological Activities Many therapeutic uses have been reported in African tradi-
tional medicine; these include: anticancer properties, anti-psoriasis conditions, anti-
microbial properties, and anti-inflammatory properties (Saini et al. 2009; Oyedeji
and Bankole-Ojo 2012; Oyelami et al. 2012).
Maytenus buchananii (Loes.) Wikzek.
Family, CELASTRACEAE
Synonyms None.
Geographical Distribution The genus is endemic in the Eastern, Central and
Southern Africa.
Habitat and Ecology An evergreen perennial shrub usually found in the forest
habitat.
Chemical Constituents The chemical constituents include the following:
Pristimerin; The ANSA MACROLIDES which include: Maytansine, Maytanprine,
Maytanbutine (USA Dept of Agric 1978).
Uses in Traditional Medicine The roots are employed for the treatment of rheu-
matoid arthritis and cancer.
Biological Activities Anticancer properties (USA Dept of Agric 1978; Handbook
of African Meduicinal plants).
Mondia whitei (Hook.f.) Skeels
Family, APOCYNACEAE
Synonyms Common names: White’s ginger; Tonic root.
Geographical Distribution The plant is native to all parts of Africa.
Habitat and Ecology The plant is a perennial, woody climber with a tuberous
rootstock. The roots are aromatic.
Chemical Constituents The chemical compounds isolated so far include
2-hydroxy-4-methoxybenzaldehyde; methyl salicylate; beta-sitosterol; alpha-beta-
amyrin acetate (Watcho et al. 2007; Malviya et al. 2011; Watcho et al. 2012).
Uses in Traditional Medicine Enhancement of sexual function in the male;
Treatment of erectile dysfunction;
Biological Activities The aphrodisiac. properties have not been clinically proved.
Prunus Africana (Hook.f.) Kalkman,
Family, ROSACEAE
Synonyms Pygeum africanum, Stinkwood.
Geographical Distribution Prunus Africana can be found in the forests of coun-
tries of Africa south of the Sahara. It is most common in the congo basin, eastern
and southern Africa (Cunningham and Mbenkum 1993; Thorne Research Inc 2002;
Kadu et al. 2012).
Habitat and Ecology It is an evergreen tree that is native to montane regions of
and the neighboring African islands. It grows at altitudes between 900 and 3400 m,
reaching 25 m high.
Chemical Constituents Phytosterols; Pentacyclic triterpenoids; long chain ali-
phatic alcohols; long fatty acids; Trans-ferulic acid esters (Thorne Research Inc
2002; Kadu et al. 2012).
Uses in Traditional Medicine The stem bark is used in combination with other
plant materials in traditional medicine.
Biological Activities The bark extracts have been used for the treatment of benign
prostate hyperplasia.
Sclerocarya birrea (A. Rich.) Hochst.
Family, ANACARDIACEAE
Synonyms Poupartia birrea (A. Rich.) Aubrev.; Spondias birrea (A. Rich.),
Common name, Marula.
Geographical Distribution The plant is native to the following countries:
Botswana, Democratic Republic of Congo, Eritrea, Ethiopia, Gambia, Kenya,
Malawi, Mozambique, Namibia, Niger, Senegal, Somalia, South Africa, Sudan,
Swaziland, Tanzania, Uganda, Zambia, Zimbabwe.
Habitat and Ecology It is a medium-sized tree with a wide spreading crown and
grows to 18 m high. It grows well in dry land habitat.
Chemical Constituents Oils from seeds.
Uses in Traditional Medicine The bark and fruits are used.
Biological Activities Antiparastic and antimicrobial properties; Gastrointestinal
and antihypertensive activities (Hall et al. 2002; Wynberg et al. 2002; Mariod and
Abdelwahab 2012).
Sutherlandia frutescens (L.) R.Br.
Family, FABACEAE
Synonyms Colutea frutescens L.; Lessertia frutescens (L.) Goldblatt & JC
Manning.; Cancer bush.
Geographical Distribution South-west of the Western Cape of South Africa.
Habitat and Ecology This is a small plant that grows wild as bush to about 1 m
high.
Chemical Constituents A triterpene called SU1, Canavanine, a polyhydroxy
cyclitol, pinitol; and gamma-Aminobutyric acid, GABA (Chinkwo 2005; Van Wyk
and Albrecht 2008).
Uses in Traditional Medicine The leaves are used to prepare medicines for
cancer.
Biological Activities Treatment of the following conditions: HIV/AIDS, cancer,
diabetes, stress, inflammatory diseases (Chinkwo 2005; Van Wyk and Albrecht
2008).
Tabernanthe iboga (L.) Nutt.
Family, APOCYNACEAE
Synonyms Iboga.
Geographical Distribution West-Central Africa: Gabon, Cameroon, Republic the
Congo.
Habitat and Ecology A perennial rainforest shrub growing to 2 m, although it
may under favourable conditions, grow to 10 m high.
Chemical Constituents Indole alkaloids, Ibogaine and derivatives (Alper 2001;
Daniel 2006; Koenig and Hilber 2015).
Uses in Traditional Medicine Roots and stem bark are used in Bwiti cultural
traditions.
Biological Activities Effects on the central nervous system; treatment of effects
due to heroin withdrawal and similar effects of other drugs (Koenig and Hilber
2015).
Toddalia asiatica (L.) Lam.
Family, RUTACEAE
Synonyms Paullinia asiatica.
Geographical Distribution This plant is native to many countries in Africa.
Habitat and Ecology It is woody and thorny climber reaching up to 10 m in length.
Chemical Constituents Benzophenanthridine Alkaloids. Nitidine, Chelerythrine,
Norchelerythrine, Chelerythrine_cyanide, Avicine (Timberlake and Kativu 1999;
Rajkumar et al. 2008; Nyahanga et al. 2013).
Uses in Traditional Medicine The roots are used in traditional treatment of
malaria and sickle cell anemia.
Biological Activities No confirmed clinical studies have been done.
Voacanga africana Stapf,
Family, APOCYNACEAE
Synonyms Voacanga.
Geographical Distribution The plant is native to most countries in Africa.
Habitat and Ecology This is a small tropical tree that grows up to 6 m in height in
the forest vegetation.
Chemical Constituents The plant contains indole alkaloids which include voac-
angine and Ibogaine (Thomas and Biemann 1968; De Ruijter 2008).
Uses in Traditional Medicine The roots, stem bark and leaves are used in tradi-
tional medicine.
Biological Activities The constituents have been demonstrated to exhibit anti-
inflammatory, and antioxidant effects.
5 Conclusions
The above account has summarized the situation analysis on medicinal and aromatic
plants in Africa with particular reference to their usefulness in addressing their
application in providing health on the African continent. In the short term, the
account has identified some areas which would need to be addressed whilst adopting
the herbal medicine for the incorporation strategy. The strategy has raised some
fundamental issues on implementation. On the other hand, there is abundant evi-
dence of the therapeutic usefulness of medicinal and aromatic plants in therapy.
References
Agyare C, Dwobeng AS, Agyepong N, Boakye YD, Mensah KB, Patrick George Ayande P,
Adarkwa-Yiadom M (2013) Antimicrobial, antioxidant, and wound healing properties of
Kigelia africana (Lam.) Beneth. and Strophanthus hispidus DC. Adv Pharmacol Sci 2013:
Article ID 692613, 10 pages. Available at: http://www.hindawi.com/journals/aps/2013/692613/.
Accessed on 13 May 2015
Alper KR (2001) Chapter 1: Ibogaine: a review. In: The alkaloids, vol 56. Academic, San Diego,
pp 1–38
Anjum T, Hasan Z (2013) Gymnema sylvestre. Plant used by peoples of Viidisha District for the
treatment of diabetes. Int J Eng Invent 2:98–102
Boukes GJ, Van de Venter M, Oosthuizen V (2008) Quantitative and qualitative analysis of ste-
rols/sterolins and hypoxoside contents of three Hypoxis (African potato) spp. Afr J Biotechnol
7(11):1624–1629
Burkill HM (2010) The useful plants of West Tropical Africa, complete set of volumes 1–6, 2nd
edn. Royal Botanic Gardens, Kew. ISBN-13:9781842461327
Chinkwo KA (2005) Sutherlandia frutescens extracts can induce apoptosis in cultured carcinoma
cells. J Ethnopharmacol 98:163–170
Cunningham AB, Mbenkum FT (1993) People and plants working paper 2 May, Geographical
distribution and values of Prunus Africana, pp 4–11
Daniel M (2006) Medicinal plants. Chemistry and Properties. Science Publishers, – Science – 250
pages
De Ruijter A (2008) VOACANGA AFRICAN A Stapf. In: Medicinal plants – vol 1 – Page
623. Protologue J Linn Soc Bot 30:87 (1894). Available at: https://books.google.cg/
books?isbn=9057822040. Accessed on 14 May 2015
European Food Safety Authority (2009) Guidance on safety assessment of botanicals and botanical
preparations intended for use as ingredients in food supplements
Gabriel OA, Olubunmi A (2009) Comprehensive scientific demystification of Kigelia Africana: a
review. Afr J Pure Appl Chem 3(9):158–164
Gachathi FM (2007) Kikuyu botanical dictionary, Revised 2nd edn. Tropical Botany, Gituamba
Gajalakshmi S, Vijayalakshmi S, Devi Rajeswari V (2013) Pharmacological activities of
Catharanthus roseus: a perspective review. Int J Pharm Bio Sci 4(2):431–439
Hall JB, O’Brien EM, Sinclair FL (2002) Sclerocarya birrea: a monograph. School of Agricultural
and Forest Sciences Publication Number 19, University of Wales, Bangor. 157 pp (Edited by
John B. Hall, E. M. O’Brien and Fergus L. Sinclair)
Iwu M (1993) Handbook of African Medicinal plants. CRC Press, Boca Raton, Feb 18, – Science –
464 pages.
Kadu CAC, Parich A, Schueler S et al (2012) Bioactive constituents in Prunus africana:
Geographical variation throughout Africa and associations with environmental and genetic
parameters. Phytochemistry 83:70–78
Kebenei JS, Ndalut PK, Sabah AO (2011) Synergism of artemisinin with – V from Erythrina abys-
sinica (Lam ex) against Plasmodium falciparum parasites: a potential anti-malarial combina-
tion therapy. J Med Plants Res 5(8):1355–1360
Koenig X, Hilber K (2015) The anti-addiction drug Ibogaine and the heart: a delicate relation.
Molecules 20:2208–2228
Kokwaro JO (2009) Medicinal plants of East Africa, 3rd edn. University of Nairobi Press, Nairobi
Malviya N, Jain SS, Gupta VB, Vyas S (2011) Recent studies on aphrodisiac herbs for the man-
agement of male sexual dysfunction – a review. Acta Poloniae Pharmaceutica ñ Drug Research
68(1):3–8
Mariod AA, Abdelwahab SI (2012) Sclerocarya birrea (Marula), an African tree of nutritional and
medicinal uses: a review. Food Rev Int 28:375–388
Mkhize N, Mohantalall V, Odhav B (2013) Isolation and Quantification of β-sitosterol, ergosterol

and stigmasterol from Hypoxis rigidula Baker var. rigidula and Hypoxis hemerocallidea Fisch.,
C.A.Mey. & Avé-Lall (Hypoxidaceae). Int J Sci 2:118–134
Najafi S, Deokule SS (2011) Studies on gymnema sylvestre – a medicinally important plant of
the family asclepiadaceae. Trakia J Sci 9(2):26–32. Available online at: http://www.uni-sz.bg.
Accessed on 14 May 2015
Nyahanga T, Jondikoa JI, Manguroa LOJ, Orwa JA (2013) Antiplasmodial and larvicidal com-
pounds of Toddalia asiatica root bark. J Sci 125(5):1115–1121
Oyedeji FO, Bankole-Ojo OS (2012) Quantitative evaluation of the antipsoriatic activity of sau-
sage tree (Kigelia Africana). Afr J Pure Appl Chem 6(13):214–218
Oyelami OA, Yusuf KO, Oyelami AO (2012) The use of Kigelia Africana in the management of
polycystic ovary syndrome (PCOS). Chin Med 3:1–3
PORTA: Erythrina abyssinica (PROTA). Available at: http://uses.plantnet-project.org/en/
Erythrina_abyssinica_%28PROTA%29. Accessed on 14 May 2015
Putheti R, Okigbo RN (2008) Effects of plants and medicinal plant combinations as anti-infectives.
Afr J Pharm Pharmacol 2(7):130–135
Rajkumar M, Chandra RH, Kaleab Asres K, Veeresham C (2008) Toddalia asiatica (Linn.) Lam. –
a comprehensive review. Pharmacogn Rev 2(4):386–397
Sain M, Sharma V (2013) Catharanthus roseus (an anti-cancerous drug yielding plant) – a review
of potential therapeutic properties. Int J Pure Appl Biosci 1(6):139–142
Saini S, Kaur H, Verma B, Ripudaman, Singh SK (2009) Kigelia Africana (Lam.) Benth. – an
overview. Nat Prod Radiance 8(2):190–197
Thomas DW, Biemann K (1968) The hydroxyindolenine derivative of Voacangine, a new indole
alkaloid from Voacanga africana. Tetrahedron 24:4223–4231
Thorne Research Inc (2002) Monograph. Pygeum africanum. Altern Med Rev 7(1):71–74
Timberlake J, Kativu S (1999) African plants: biodiversity, taxonomy and uses: proceedings of
the 1997 AETFAT congress, Harare, Zimbabwe, 581 pages. Royal Botanic Gardens, Kew
Tiong SH, Looi CY, Hazni H et al (2013) Antidiabetic and antioxidant properties of alkaloids from
Catharanthus roseus (L.) G. Don. Molecules 18:9770–9784
USA DEPT OF AGRIC (1978) Review of taxonomy, distribution and ecology of Maytenus bucha-
nanii. Maurice Iwu M (1993)
Van der Heijden R, Jacobs DI, Snoenijer W, Hallard D, Verpoorte R (2004) The Catharanthus
alkaloids: pharmacognosy and biotechnology. Curr Med Chem 11:607–628
Van Wyk B-E, Albrecht C (2008) A review of the taxonomy, ethnobotany, chemistry and pharma-
cology of Sutherlandia frutescens (Fabaceae). J Ethnopharmacol 119:620–629
Wabel NT (2011) Psychopharmacological aspects of Catha edulis (Khat) and consequences of
long term use: a review. J Mood Disord 1(4):187–194
Watcho P, Djoukeng C, Zelefack F et al (2007) Relaxant effect of Mondia whitei extracts on iso-
lated Guinea Pig Corpus Cavernosum. Pharmacol Online 2:44–52
Watcho P, Zelefack F, Ngouela S et al (2012) Enhancement of erectile function of sexually naïve
rats by beta-sitosterol and alpha-beta-amyrin acetate isolated from the hexane extract of
Mondia whitei. Asian Pac J Trop Biomed. S1266–S1269
Watt JM, Breyer-Brandwijk MG (1962) The medicinal and poisonous plants of Southern and
Eastern Africa, 2nd edn. E & S Livingstone Ltd, Edinburgh/London
WHO (1980) KHAT: pharmmacological aspects. UN Bulletin on Narcotics, Issue 3
WHO Expert Committee on Drug Dependence (2006) Assessment of khat (Catha edulis. Forsk).
34th report. WHO Technical Report Series No. 942, pp 1–25
World Health Organization (2001) Promoting the role of traditional medicine in health systems: a
strategy for the African Region (Strategy documents, AFR/RC50/9 and Resolution AFR/RC65/
R3). WHO Regional Office for Africa, Harare, Zimbabwe
World Health Organization (2013) Enhancing the role of traditional medicine in health systems: a
strategy for the African Region (Strategy documents, AFR/RC63/6 and Resolution AFR/RC65/
R3). WHO Regional Office for Africa, Brazzaville, Republic of the Congo
Wynberg R, Cribbins J, Leakey R et al (2002) Knowledge on Sclerocarya birrea subsp. caffra with
emphasis on its importance as a non-timber forest product in South and southern Africa: a sum-
mary. South Afr For J 196:67–70
Yenesew A, Twinomuwhezi H, Kiremire BT et al (2009) 8-Methoxyneorautenol and radical scav-
enging flavonoids from Erythrina abyssinica. Bull Chem Soc Ethiop 23(2):205–210
Some Important Aromatic and Medicinal
Plants of Morocco
Saadia Zrira
Abstract The most important characteristic of the Moroccan Aromatic and

Medicinal Plants sector is its diversity and its richness. Because of the ecological
heterogeneity and climatic variations, 800 of the more than 4200 plant species
growing in this country are of aromatic and/or medicinal interest. This diversity is
more marked when compared with other Mediterranean countries: Algeria (3150),
Tunisia (2200), Libya (1800), Egypt (2100), Mauritania (1100), Lebanon (2100),
Jordan (2500), Syria (2100) and Portugal (3100). In the European countries France
and Spain, the number of native species is 4500 and 4900, respectively. Turkey is
the country in this region that shelters the greatest number of species (9000). The
Moroccan production of herbs and their extracts comes from both wild crafted and
farmed species. It is estimated that only 280 species are currently evaluated. Almost
100 species are exported in the form of dried herbs for food herb trade. More than
20 species are used for the production of essential oils or other aromatic extracts
intended primarily for the perfumery and cosmetic industry and for the preparation
of hygienic products and the formulation of flavours.
Keywords Morocco • Biodiversity • Aromatic and medicinal plants • Argania spi-

nosa • Artemisia herba-alba • Cedrus atlantica • Laurus nobilis • Laurus azorica •
Myrtus communis • Rosa damascena • Rosmarinus officinalis
1 Introduction
Morocco is a traditional supplier of the world market in aromatic and medicinal

plants. This activity concerns the exploitation of both wild-crafted and cultivated
species. Several products (more than 70) are exported in the form of dried plants for
the food herbs trade. More than twenty species are used for the production of essen-
tial oils or other aromatic extracts intended primarily for the perfumery and
S. Zrira (*)
Département des Sciences Alimentaires et Nutritionnelles, Institut Agronomique et
Vétérinaire Hassan II, BP 6202, Rabat Instituts, Rabat, Morocco

DOI 10.1007/978-94-024-1120-1_5
92 S. Zrira
cosmetic industry and also for the preparation of hygienic products and flavour
formulations (Zrira 2011a, b).
The harvesting of wild growing species represents more than 90% of national
production. The number of wild-crafted species is large. Their most important rep-
resentatives are rosemary, thyme, pennyroyal, Artemisia, oregano, myrtle and carob
seeds.
The wild-crafted species can be classified into the following three categories:
• Plants grown in the forest, such as rosemary and myrtle. In order to exploit these
resources, it is necessary to have authorisation from the Moroccan forestry
administration, either after a deal or by direct negotiation. In both cases the
recipient pays a royalty to rent the right to exploit an area for 3 years.
• Plants grown on local communities’ land. The exploitation of this resource is
subject to the same requirements and same steps. The only difference lies in the
ministry responsible for the supervision; in this case it is the Moroccan Interior
Ministry. Artemisia is typically exploited in this way. Plant harvesting is under-
taken by the local population.
• Plants grown on private land include pennyroyal, oregano, the annual species
Tanacetum annuum, Ormenis, etc. In general, harvesting is carried out by the
local population without any particular administrative procedures.
The most important cultivated species are verbena, coriander, sage, mint, laven-
der, geranium, bergamot, citrus, basil and saffron (Zrira 2010a, 2011a, b).
In this chapter, we will give a brief description of some important species in
Morocco such as: Argania spinosa, Artemisia herba-alba, Cedrus atlantica, Laurus
nobilis, Rosa damascena, R. centifolia and Rosmarinus officinalis.
2 Argan (Argania spinosa L.)
The Argan tree (Argania spinosa (L.) Skeels; Sapotaceae is an 80-million-year-old

relic tree species that has been known since the time of the Phoenicians, who estab-
lished trade centers throughout the Mediterranean Sea and along the coast of
Morocco, in 600 BC. It is generally accepted that all Argan trees disappeared from
Northern Africa during the Quaternary glaciations, except in the Souss valley where
optimum conditions for the trees’ survival remained (Kenny and De Zborowski
2007). The Argan tree is the only species of the family Sapotaceae that grows in the
subtropical zone. The Argan forest covers about 8280 km2, mostly in the dry low-
lands of the Souss valley and on the sunny mountain spurs of the Anti-Atlas. The
Argan tree grows very slowly. It takes 15 years to mature. But it is extremely resis-
tant. Argan trees can live for 150 years, and sometimes more than 200 years, and
because of its deep root system, it can survive long periods of drought (Kenny and
De Zborowski 2007; Morton and Voss 1987). Therefore, the Argan tree is often the
ultimate warrior when the desert is encroaching on the Souss valley.
Some Important Aromatic and Medicinal Plants of Morocco 93
Picture 1 Argan tree
From an ecologic and economic standpoint, the Argan tree (1) stabilizes the soil,
reduces erosion, maintains moisture in the air, shelters a large variety of small and
wild animals, and (2) shades domestic cultures and furnishes via its fruit the major
part of the Argan forest dwellers lipid diet. The quasi-symbiotic development
between the Souss valley, the Argan forest and its dwellers has resulted in an equili-
brated rural micro-society that has been lasting for centuries but that is totally
dependent on the Argan tree (Achhal et al. 1980).
Taxonomy and Distribution
The Argan tree, Argania spinosa (L.) Skeels belongs to a tropical family, Sapotaceae,
which includes about 10 genera and 600 species. Specifically endemic, Moroccan
Argan tree is a fruit, forest tree whose size hardly exceeds 8–10 m whose height,
dense and rounded, has spiny branches (Picture 1) (M’Hirit et al. 1998).
Leaves alternate, lanceolate, dark green on the upper surface and clear in below,
are subpersistant. The hermaphrodite flowers are pretty and complete (Picture 2).
The fruit, called “Argan nut” is a green bay of variable size and shape containing
a seed (Picture 2). Though raised by several authors (Boudy 1951; Boulos 1983;
Dupin 1949; Emberger 1938; Rieuf 1962), taxonomy of the Argan tree has not been
biosystematically studied to better define the degree of polymorphism of this
species.
The flowering is in May–June. The species is drought resistant, shedding foliage
and remaining in a state of dormancy for several years during prolonged drought.
Production of fruits is at its maximum when the tree is 60 years old. The average
yield of fruit per tree is estimated to be 8 kg per annum.
The current geographical area of Argan covers more than 800,000 ha in the
southwest Morocco, extending from Safi at north to Saharan coast. This area is one
of the most remarkable part of Moroccan territory, both by its flora and vegetation.
94 S. Zrira
Picture 2 Botanical
characteristics of Argan
tree (a) branch with
inflorescences; (b) branch
with fruit; (c) flower; (d)
seed
In northern, two stations quite special: that of Oued Grou near Rabat, and that of the
bulk of Beni Snassen near Oujda, reflect the extension of the Argan in tertiary and
early Quaternary.
Ecology
The trees cling to the slopes of rough hills and seem to thrive between the rocks on
poor soil. Growing up to an elevation of 1500 m, they only need annually from 100
to 200 mm of rain. Unable to withstand cold, the Argan tree is resistant to extreme
heat and drought, making it ideal for the climate of the arid southeastern Moroccan
coast. The associated species in the Argania woodlands includes Periploca laevi-
gata, Senecio anthephorbium, Launaea arborescens, Warionia saharae, Acacia
gummifera, Balanites aegyptiaca, Maerua crassifolia, Rhus trpartitum, Withania
frutescens, Euphorbia officinarum, Cytisus albidus, Ephedra altissima and
Tetraclinis articulata.
The mean annual temperature is 18–20 °C and the mean annual rainfall is 100–
500 mm. The altitude is 0–1500 m.
Soil type: It is well suited to calcareous soils, sandy deposits and relatively poor
semi desert soils conditions but not drifting sands and water-logged soils (Debouzie
and Mazih 1999).
The Argan-Oil
The Argan tree (Argania Spinosa (L.)) Skeels provides a nut sized fruit containing
kernels that are used to produce a range of cosmetic and edible Argan-oils available
for national and export markets. Argan-oil has a high percentage of unsaturated fatty
acids, which makes it of high nutritional quality (Yaghmur et al. 1999; Khallouki
et al. 2003). It is relatively stable in storage (Khallouki et al. 2003; Farines et al.
1981; Chimi et al. 1994) and frying (Yaghmur et al. 2001) because of its natural
antioxidants, mainly tocopherols.
In Argan-oil production, the ripe fruit pulp and peel are discarded and the nuts
are carefully broken manually with stones to separate the kernels (or “almonds”).
Argan-oil extracted from raw kernels by press extraction is referred as cosmetic
grade while edible Argan-oil is extracted from roasted kernels. Kernel roasting is
used to give the oil its desirable organoleptic characteristics and to improve oil
extraction yield. Edible Argan-oil, can be produced by a traditional (manual), or a
semi mechanical process. In the traditional technology, women use a multi-step
process that includes kernels roasting in clay containers followed by grinding the
roasted kernels using a millstone to give a brownish viscous mix. The mix is hand-
worked with the concomitant addition of water to give dough, which is, and then
hand pressed to give an emulsion and a cake. Oil is separated from the emulsion by
decantation. This hand-press oil extraction technology is time consuming which
gives low oil yields and a product with poor shelf-life (Charrouf and Guillaume
1999). According to the mechanical technology, kernels are roasted in a rotating
oven using gas burners at the base of the cabinet for heat followed by the extraction
of the oil by mechanical pressing. The introduction of the mechanical technology by
several women cooperatives and industrial units has improved oil quality and extrac-
tion yield, and significantly reduced the time required for oil extraction (Charrouf
and Guillaume 1999; Fellat et al. 1987).
Chemical Composition of Argan-Oil
The edible Argan-oil density at 20 °C, relative to the density of water at the same
temperature, ranges from 0.906 to 0.919. At the same temperature, its refractive
index is 1.463–1.472, and its acid value between 0.8 and 2.5 [Service de normalisa-
tion industrielle (SNIMA) 2003]. The acid value makes it easy to distinguish extra-
virgin Argan-oil, fine-virgin Argan-oil, virgin Argan-oil, and lampante Argan-oil;
the acid value of extra-virgin Argan-oil must be lower than 0.8 (Service de normali-
sation industrielle (SNIMA 2003). The variability of the extra-virgin Argan-oil acid
value has been studied as a function of its various parameters. Together with the
seed origin, the extraction technology is also a parameter possibly modifying the
Argan-oil acid value (Hilali et al. 2005). Comparing Argan-oil samples prepared
from roasted versus non-roasted kernels, mechanically peeled versus goat-digested
fruits, and fruits originating from different geographical regions, the acid values
ranged between 0.15 and 0.9 (Hilali et al. 2005).
96 S. Zrira
Acylglycerols, including 95% of triacylglycerols, constitute 99% of the Argan-

oil. The remaining 4% are composed of monoacylglycerols (0.27–0.65%), diacylg-
lycerols (0.68–1.53), and free fatty acids (1.1–2%) (Maurin 1992). The two main
fatty acids found in Argan-oil acylglycerols are oleic acid (O) (46–48%) and lin-
oleic acid (L) (31–35%), α mono- and α-diunsaturated fatty acid, respectively. The
third and fourth main fatty acids are palmitic acid (P) (11–14%) and stearic acid (S)
(4–7%) (SNIMA 2003; Hilali et al. 2005). These two latter acids are saturated fatty
acids.
The major triacylglycerols found in Argan-oil include two or more oleic acid
residues. Other frequently encountered triacylglycerols include two linoleic acid
residues and one oleic acid moiety or two oleic acid residues and one palmitic acid
moiety (Rahmani 2005).
Unsaponifiable matter constitutes 1% of Argan-oil. It is made of carotenes (37%),
tocopherols (8%), triterpene alcohols (20%), sterols (29%), and xanthophylls (5%).
In extra virgin Argan-oil, the levels of tocopherols are between 600 and 900 mg/kg.
The main tocopherol found in Argan-oil is γ-tocopherol (between 81 and 92%); it is
a strong antioxidative agent. α-, β-, and δ-tocopherols represent 2.4–6.5%, 0.1–
0.3%, and 6.2–12.8%, respectively, of the total tocopherol fraction. Other phenols
have also been identified as traces using mass spectroscopy; they might also act as
antioxidants. These phenol derivatives include caffeic acid, oleuropein, vanillic
acid, tyrosol, ferulic acid, syringic acid, catechol, resorcinol, (−)-epicatechin, and
(1)-catechin (Rojas et al. 2005; Charrouf and Guillaume 2008). Five ubiquitous tri-
terpene alcohols have also been identified: tirucallol (27.9%), β-amyrine (27.3%),
butyrospermol (18.1%), lupeol (7.1%), and 24-methylene cycloartanol (4.5%)
(Charrouf and Guillaume 2008).
Edible Argan-oil has a rich aroma and flavor that confer it a high culinary value
as a seasoning and cooking oil. Pyrazines, aldehydes, ketones, hydrocarbons, alco-
hols, pyrroles and furans were the main aroma compounds of commercial edible
Argan-oils (Zahar et al. 2007). In addition, qualitative and quantitative differences
in aroma profile were observed between commercial oils and these differences were
attributed to differences in the roasting step and/or extraction techniques used in
their preparation. In a study of the effect of oil extraction on the quality of edible
Argan-oil during storage, Matthaus et al. (2010) found no change in the sensory
characteristics of Argan-oil obtained by mechanical extraction after 20 weeks at
20 °C; however, the oil obtained by traditional extraction, developed a Roquefort
cheese taste after 12 weeks of storage.
These authors found also that sensory attributes of oil from unroasted seeds
changed very fast and within 4 weeks attributes like moldy/musty and Roquefort
cheese taste appeared. Hilali et al. (2005) found that the processing method had no,
or little, influence on the overall oil composition (fatty acid profile, antioxidants,
pigments, etc.) and that both press-extracted and traditionally prepared oil had simi-
lar compositions. However, oils obtained from roasted seeds had lower acidity val-
ues and α-tocopherol contents than oils extracted from non-roasted seeds.
Zahar et al. (2011) found that aroma compounds identified in Argan-oil prepared
from unroasted kernels (cosmetic grade) were limited in number and dominated by
alcohols and few aldehydes and ketones. Volatile compounds identified in edible
Argan-oils prepared from roasted kernels were derived primarily from Maillard
reaction, lipid oxidation, sugar degradation and/or their interactions. Edible oils
prepared by the traditional manual process or the semi-mechanical process had
similar aroma profiles but they showed differences in aroma compounds levels. The
oil obtained by the semi-mechanical process contained significantly (p < 0.05)
higher levels of pyrazines, aldehydes and ketones than the edible traditional oil. No
significant differences were observed for furans, furanones, pyrroles, sulfuric com-
pounds and terpenes between the two edible oils.
The olfactometric profile of cosmetic grade oil revealed mainly fruity/green/
herbal notes. In edible Argan-oils, in addition to odours detected in the cosmetic
grade oil, roasty/toasty/nutty and burnt sugar/caramel notes were also present.
Traditional oil presented more fruity and burnt sugar/caramel notes than the oils
obtained by the semi-mechanical process.
Medicinal Properties of Argan-Oil
The Argan-oil, extracted from Argan-tree fruits, has been known for its various
pharmacological properties and used in traditional medicine like a natural remedy
since several centuries. Argan-oil is traditionally used for skin, nail, and hair care,
cooking, massage and healing. Its chemical composition highlights the interest of
many laboratories to use it in their best selling products. The remarkable properties
of Argan-oil evaluated by numerous laboratories are: restoration of the skin water-
lipid layer and an increase of nutrients in the skin cell, stimulation of intracellular
oxygen, neutralization of free radicals, and protection of the conjunctive tissue.
Recently, various studies were realised in vitro or on human and animal models sug-
gesting that Argan-oil could play a beneficial role in cardiovascular prevention and
its consumption could protect against atherosclerosis via a variety of biological
mechanisms. Virgin Argan-oil is characterized by high contents of antioxidants and
mono and polyunsaturated fatty acids. These data, in addition to its cosmetic bene-
fits give to Argan-oil a unique value as natural source with high economic value
(Adlouni et al. 2009).
3 Artemisia herba-alba Asso.
Botanical Description
Artemisia herba-alba is an herbaceous plant with woody and branched stems,

30–50 cm, very leafy with thick stock. The leaves are small, sessile, pubescent and
silvery appearance. The flowers are grouped in clusters, with very small heads
(3/1.5 mm) and ovoid. The involucre bracts are overlapping the orb and pubescent
98 S. Zrira
Picture 3 Artemisia herba-alba
outside. The floral receptacle is naked with 2–5 yellowish flowers per head all her-
maphrodites (Picture 3).
Distribution and Ecology
The largest populations of this species are found in the arid and semi-arid biocli-
matic zones. They grow on various types of soils that are generally rich in lime-
stone. Artemisia herba-alba leak sites where rainwater accumulates and stagnates
(Benjilali and Richard 1980).
The sagebrush stands cover an estimated area of 10 million hectares in North
Africa. This is the most common aromatic plant in Morocco. It occupies about
2.5 million hectares. The sagebrush stretches on the high plateau of the East, the
High Moulouya, the foothills south of the Atlas and the valley of Oued Souss, with
representatives in the arid western region of Haouz and arid valleys High Atlas and
Souss continental. It grows, both on the plates in the plains and troughs.
Artemisia herba-alba is a Xerophil chamephyte; it forms more or less open
steppes. Its range, extends from Spain to the Middle East, via North Africa and
Egypt; it is also present in Iran, Iraq and Afghanistan.
In Morocco, it prefers hot, dry climates, where it is widespread in many areas
and especially in the blast eastern plateaus, the region of Boulmane, Midelt, High
Atlas and Anti-Atlas (Bruneton 1993; Fennane 1987).
Chemical Composition of the Essential Oil
The qualitative and quantitative analysis of Artemisia herba-alba essential oils

shows an extraordinary polymorphism. Sixteen “chemical varieties” (chemotypes)
were distinguished in Moroccan A. herba-alba. The most important chemotypes are
as follows:
• α-thujone chemotype: Essential oils of that chemotype are characterized by a

high content of α-thujone, usually greater than 50% and may reach 80%. This
chemotype characterized A. herba-alba of the Dades Valley and the plain of
Rachidia.
• β-thujone chemotype: This chemotype is characterized by a high content of
β-thujone (50%–80%). It is especially abundant in the region of Boulmane and
Haute Moulouya.
• Chemotype with camphor: In this chemotype, camphor alone accounts for over
50% of the total essential oil. It’s found in the Anti-Atlas and Souss region.
• Davanon chemotype: The essential oil of this chemotype is rich in davanon (40–
70%). This chemotype is found in the region of Saghro and High Atlas.
• Chrysanthenone chemotype: The major constituent is chrysanthenone. This che-
motype is scarce. It is found mainly in the Upper Moulouya and the North-East
of Morocco.
• Chrysanthényle acetate chemotype: In this chemotype chrysanthenyle acetate
can reach 60% of the essential oil. It’s a relatively rare chemotype, which is
found in the northeast of the country.
• α-thujone-camphor chemotype: This chemotype is characterized by an average
content of about 30% in α-thujone, camphor 36% and 6% β-thujone. A. herba-
alba of Marrakech is characteristic of this chemotype (Benjilali et al. 1982).
It’s important to notice that the commercial A. herba-alba essential oil is the
α-thujone/camphor chemotype (32 and 37% respectively) (Benjilali et al. 1984,
1985, 1986; Bellakhdar 1978; Bellakhdar et al. 1987).
Use of A. herba-alba and Its Essential Oil
In Morocco, Artemisia herba-alba is used extensively both in traditional medicine

to treat helminthiasis, diabetes mellitus and other conditions such as jaundice. Also,
the antihyperglycaemic, antimicrobial, antioxidant, antispasmodic, anti-venom,
nematicidal, anthelmintic, anti-leishmanial, neurological, pesticidal and antibiotic
resistant inhibitor activities of this plant have previously been reported (Imelouane
et al. 2010; Mohamed et al. 2010). Moreover, the aqueous extract significantly
increased gastrointestinal transit time and the reaction time to thermal stimuli
(Husnia Marrif et al. 1995). Furthermore, the species of this genus are widely used
in the pharmaceutics, cosmetics and food industry. They are also used to treat hair
and the preventive effect of diseases of the cold season, mainly colds and rheuma-
tism. They are also fortifying species, antiseptic, healing and warming character. A
decoction or infusion based on Artemisia species can relieve abdominal pain, vomit-
ing and digestive disorders. This recipe is also recommended in the case of fever,
diarrhea, upset stomach, and liver. The same preparation is also used as an
anthelmintic.
100 S. Zrira
Artemisia herba-alba Asso, known also as desert wormwood (known in Arabic

as shih, Armoise blanche (Fr.)), has been used in folk medicine by many cultures
since ancient times. In Moroccan folk medicine it has been used to treat arterial
hypertension and/or diabetes. Herbal tea from this species has been used as analge-
sic, antibacterial, antispasmodic, and hemostatic agents. This plant is also suggested
to be important as a fodder for sheep and for livestock in the plateau regions of
Morocco where it grows abundantly.
4 Atlas Cedar (Cedrus atlantica Mannerti)
Taxonomy
The genus Cedrus, family Pinaceae, subfamily Abieteas, is an old genus, known
since the tertiary. Widespread, it includes several species whose taxonomic value
has long been controversial. First integrated into a single species (Cedrus libanotica
Link), cedars evolved into the following four species, depending on biogeographi-
cal conditions:
• The Atlas cedar (Cedrus atlantica Manetti)
• The Lebanon cedar (Cedrus libani Loudon)
• Cyprus Cedar (Cedrus brevifolia Henry);
• Himalayan cedar (Cedrus deodora Loudon) (M’Hirit 2006).
Morphological Description
It’s a majestic allure tree that can easily exceed 40 m in height (Picture 4). Its lon-
gevity is important; the branches are very long and horizontal. The needles of
25 mm, bouquet on short shoots, green or glaucous, apex acute. The cones of 5–8 cm
are cylindrical flat-topped, green and brown (Pictures 4, 5 and 6).
Atlas cedar lives in mountainous areas and cedar forests grow between an alti-
tude of 1500 and 2500, with a preference for the northern and western slopes that
are watered; it has several local varieties, differing in size, appearance, and espe-
cially the color.
Geographical Distribution
Cedar spontaneously constitutes three distinct biogeographic blocks (M’Hirit 1982,

1994):
• North Africa, with Cedrus Atlantica (180,000 ha).
Picture 4 Atlas cedar tree
Picture 5 The cones

containing the seeds
Picture 6 The leaves of

Atlas cedar
• Minor Asia, where live two species Cedrus libani in Lebanon (1700 ha), Syria
some hundreds of hectares and more than 160,000 ha in Turkey and about ten
hectares of Cedrus brevifolia in Cyprus.
• The third non-Mediterranean bloc in Himalayas with Cedrus deodora represent-
ing large areas of India and Afghanistan (500,000 ha).
102 S. Zrira
The genus Cedrus is subdivided into four distinct species: Cedrus atlantica
Manetti, Cedrus libanotica Barr, Cedrus deodora Loudon, Cedrus brevifolia Henry
(Moufaddal 1983).
In Morocco the cedar exists in the Middle Atlas, the Rif, and the High Atlas
(M’Hirit 1982). In the Rif, cedar occurs sporadically in the Limestone Mountains of
Jbala but its density increases rapidly eastward. In the high Atlas and the Middle
Atlas, stands form two blocks, one occupies the high mountains, south of Taza, the
other is between the Middle central Atlas and the eastern High Atlas.
Ecological Requirements
Atlas cedar is a large evergreen tree gymnosperm, bole squat, whose bark, initially
smooth, greyish brown cracks with age in small brown or dark gray scales. Very
long branches spread horizontally or slightly raised, form a whole by a large conical
or ovoid crown with a wingspan can exceed 100 m. The arrow takes in older tree, a
horizontal direction. The cones are smaller (5–6 cm), often with a small hollow in
the center, yellowish green, they become purple before maturity. The leaves (nee-
dles) evergreen, leathery, acute, green or glaucous, isolated on elongated branches
gathered in dense rosette on numerous short branches that fill the entire length of the
branches. Seeds, triangular, are 10–15 mm long, dull colors are tending toward
brown and provided with long wing and are rich in resins.
Height growth of Atlas cedar is continuing into old age, at least 200–300 years.
The growth rate is weak the early years.
Chemical Composition
Morocco is the main supplier of the international market by the Atlas cedar essential
oil extracted from sawdust. It is a yellowish orange yellow liquid, viscous and
sometimes a little trouble. Its smell is very special, slightly camphor with a sweet
and woody notes reminiscent Cassie and mimosa.
The essential oil extracted from the Moroccan Cedrus atlantica sawdust is rich
in α-himachalene (17.19%), γ-himachalene (9.53%) and β-himachalene (41.69%)
and its also contains γ-Atlantone (2.92%) and Cedryl methylique cetone (3.52%)
(Zrira 2010b).
Chalchat et al. (1994) analyzed the essential oil extracted from the Moroccan
Cedrus atlantica sawdust, and identified 53 compounds. The special particularity of
this essential oil is its himachalène content in the form of three isomers: α, β, and γ,
which constitute the majority of the essential oil, compounds (70%). Other charac-
teristic compounds of this essential oil are atlantone (E and Z) are also present but
in much lower concentrations.
Based on the work done in the GEF-Rif project, essential oil extracted from the
leaves of Atlas cedar located in Ghomara region has a chemical composition that is
completely different from that extracted from sawdust. The essential oil extracted
from the thick boughs was rich in monoterpenic hydrocarbons (57%): limonene
(29.2%) ocimene + α-pinene (18.6%) and myrcene (16%).
Use of Atlas Cedar and Its Essential Oil
Cedar makes multiple services and provides useful products: lumber, firewood and
sawdust as energy, essential oils (wood, leaves and branches) and wood tar and is
home to a large pasture.
It provides a fairly brittle wood, which limits its use for framing. Due to its impu-
trescibility it is popular in shipbuilding. Cedar poles have good elasticity, prevent
and withstand before breaking sufficient evidence to be used as mine timbers.
Traditionally destined for uses structure, cedar wood has been used in woodwork-
ing, fine woodworking and running both inside and outside (El Abid 2006).
The Atlas cedar essential oils are used in perfumery for his role as fixative and its
unique scent that blends well with labdanum products (rock rose) and perfume
materials woody woody-floral type. It is used in the manufacture of scented soaps,
deodorants and other flavors (Arcander 1960).
The aromatic essential oil of Atlas cedar has antifungal, antiseptic, healing,
astringent and decongestant to respiratory system. It is reported to have also relax-
ing, draining, lymphotonic and diuretic, lipolytic (fat burning) effects. It is said to
improve the quality of the arterial network and scalp (dandruff as cade oil). It repels
mosquitoes and moths.
Among the ancient Egyptians, cedar oil and resin were added to the preparations
used to embalm mummies. Even today this natural attribute is exploited in cedar
hangers and balls hanging in wardrobes. The aromatic substances released from the
oil have excellent repellent properties against mites (Duquenois 1968).
5 Laurel (Laurus nobilis and Laurus azorica)
Taxonomy
The Laurus genus belongs to the family Lauraceae that are usually green and wild
growing trees, represented by 2000–2500 species. Popular representative of the
genus are, e.g.: cinnamon (from China, Ceylon), bay leaves, rosewood, the raven-
sara, sassafras.
The genus Laurus contains three species that are evergreen trees with very close
key (Mabberley 1997):
104 S. Zrira
Picture 7 Leaves, flowers and fruits of Laurus nobilis
–– Laurus azorica (Seub) Franco, also called laurel Azores;

–– Laurus nobilis L., commonly known as the “Noble Bay Laurel”, “Sauce Laurel”,
“franc laurel” or Apollon laurel is known in arabic as “‫ ”الرند‬or “‫;”ورق سيدنا موسى‬
–– Laurus novocanariensis, formerly included in Laurus azorica, is a native of
Madeira and the Canary (Costa et al. 2004).
Laurus nobilis
The laurel is a shrub native to Minor Asia and up to 10 m high. The plant has a
smooth black bark, evergreen quite variable form, alternate, coriaceous, entire, lan-
ceolate or oblong, 4–10 cm/2 to 3 cm (Picture 7). It is a dioecious plant that blooms
in early spring (April, May) in the Mediterranean countries.
The female plants produce fruits in abundance: these are black drupes, ovoid,
very bright.
Laurus azorica
A native of the Azores laurel forests, Laurus azorica also called the bay of the
Azores is a massive tree with a height of 15 m, evergreen, alternate and elliptical
4–15 cm long, smooth and dark green. Its flowers are greenish yellow. The fruits of
a length of 12 mm are black when ripe (Picture 8). The Laurus azorica leaves are
less aromatic than the laurel noble and are still used in cooking.
Picture 8 Leaves and

flowers of Laurus azorica
Laurus nobilis
Laurus nobilis is widespread in many parts of the world of warm and temperate
climate. In particular, it is abundant in the Mediterranean countries, including
Morocco, Algeria, Spain, southern France, Italy, Palestine and Cyprus.
Laurus azorica
Laurus azorica is currently stationed tree in the Atlantic islands (Canaries, Madeira,
Azores). This species also existed in Europe, where it was known as fossils in many
localities (Roiron 1979).
In Morocco, the species is found in the forest communities of Jebel Tazerkount
(Atlas Ksiba) at the forest with deciduous oaks, around 1650 m (Barbero et al.
1981), so it takes a very different ecological situation that of Laurus nobilis. It is
indeed full floor above Mediterranean deciduous at high altitudes and at minor lime-
stone cliffs (Barbero et al. 1981).
Many representatives of this tree were also observed in the Atlas near Beni
Mellal in Jebel Ighnayene. It is therefore in the same habitat, at a much higher alti-
tude, between 1900 and 2000 m. In this zone, Laurus azorica always colonizes
small limestone cliffs facing north. Deciduous oaks no longer exist at this level,
probably because of too low winter temperatures, 3 or 4 observed localities lie sub-
stantially at the upper limit of the upper Mediterranean sclerophyllous oak or moun-
tain Mediterranean.
106 S. Zrira
Laurus nobilis
Suitable for light sandy soils, loamy soil medium and heavy clay and prefers well-
drained soil. It prefers soils with acidic, neutral and alkaline pH. It can grow in the
shade or no shade half. It prefers dry or wet soils. The plant can tolerate strong
winds.
Laurus azorica
Laurus azorica occupies a very different ecological situation from Laurus nobilis.
It’s indeed, open floor above Mediterranean deciduous at high altitudes and at minor
limestone cliffs (Barbero et al. 1981).
Chemical Composition of Laurus Leaves
The leaves of Laurus nobilis contain polar flavonoids (glycosylated derivatives of

quercetin, kaempferol and catechin) and nonpolar (four acyl derivatives of kaemp-
ferol) (Fiorini et al. 1997), sesquiterpene lactones, alkaloids of isoquinoline (Simic
et al. 2003), in addition many researchers showed the richness of its leaves in vita-
min E.
By steam distillation, the leaves provide about 10–30 ml/kg (1–3%) of essential
oil (Bruneton 1999), whose major constituents include 1,8-cineole, α et β −pinene,
sabinene, linalool, eugenol, terpineol, plus other esters and terpenoids, but the pro-
portions vary by geographic origin (Sayyah et al. 2001).
The chemical composition of the essential oil of Laurus nobilis, harvested in the
region of Tétouan (Benkarrich and Oued Laou) and Laurus azorica, collected in the
region of Beni Mellal is rich in 1,8-cineole (39.0–40.2%) and (39.48–42.2) respec-
tively, linalool (3.71–8.03) and (8.1–12.25) respectively and α-terpenyle acetate
(4.67–11.86) and (2.04–6.26) respectively. They have relatively low content of
eugenol and methyl eugenol (GEF-Rif 3 1998).
Chemical Composition of Laurus Berries
• The essential oil of L. nobilis berries

100 parts of peeled berries contain 0.8% of a volatile oil obtained by steam distil-
lation of berries, 0.5 % of a particular crystalline substance, called laurine, 6.4% of
a green fatty oil, 3.5% of crystalline fat having more consistency, 0.8% a soft resin,
half fluid (containing volatile oil), 12.5% of starch, 8.6% of gum, 3.2% of plant
mucilage, 0.2% of noncrystallizable sugar, traces of vegetable albumin, 9.4% of
vegetable fiber, 0.72% of saline ash and 3.2% of water. To obtain the crystalline
substance, the berries must be exhausted with boiling alcohol and the latter is dis-
tilled. The remaining liquor allowed settling, on cooling, crystalline needles, elon-
gated and yellowish. The liquor was composed of two layers, an upper, consisted of
fatty oil, while the other was an alcoholic solution.
The fatty oil contains, like that of the leaves, 1,8-cineole in smaller amounts
(12.8%), alcohol (10.6%), esters (17.9%), cinnamic acid (1.5%), phenol (2%),
hydrocarbon terpene (15.4%) and carbonyl compounds and sesquiterpenes.
• Laurus butter
Laurel berries have the same culinary uses than the leaves; they are dried and
ground such as nutmeg. Harvested in the fall, they are rich in fats.
The berries are also used to obtain, by hot expression, fatty oil, called “laurel
butter” and whose yield is 8–9% of the berries weight. It’s a creamy green mass,
solid at room temperature, with a pleasant smell. It consists of a fat blend in which
lauric acid (54.2%), oleic acid (15.1%) and linoleic acid (17.2%) dominate.
The unsaponifiable fraction contains undecanone, α-terpineol, α-terpényle ace-
tate, β-elemene and β-sitosterol (Hafizoglu and Reunanen 1993).
Use of Laurel and Its Extracts
The leaves of Laurus nobilis are among the most popular seasoning in all countries,
they are generally used as a culinary spice (in soups, stews, sauces, etc.) and flavor-
ing agent in food industry. Laurel is mainly used orally, for the symptomatic treat-
ment of disorders of the upper digestive tract such as epigastric bloating, slow
digestion, belching and flatulence.
The aqueous extract is used in traditional Turkish medicine as an anti-
hemorrhoidal, antirheumatic, diuretic and as an antidote to snake bites and for the
treatment of stomach ache. In Iranian traditional medicine, the leaves of this plant
have been used to treat epilepsy and Parkinsonism.
Everywhere in Morocco, the infusion of the leaves is used in indigestion and
concentrated decoction is used to gargle against diseases of the oral pharyngeal
sphere (tonsillitis, mouth ulcers, bad mouth odor, gingivitis). The bay is used in
chewing or gargle decoction, against the evils of the mouth, teeth, gums or tonsils.
The leaves and berries are used as an aromatic condiment for the preparation of fish
and meat. They are also used to flavor the traditional vinegar and canned olives and
peppers.
The essential oil obtained from the leaves has been used for the relief of hemor-
rhoids and rheumatic pain (Sayyah et al. 2001). In addition, the essential oil is used
by the cosmetics industry in perfumery and in the manufacture of soaps. It is one of
the best ways to keep away annoying insects.
108 S. Zrira
Picture 9 Plant of myrtle

(Myrtus communis L.)
The bay is mainly used to treat disorders of the upper digestive system and arthri-
tis pain. Moreover, it stimulates the appetite and the secretion of gastric juices. Used
as a condiment, leaves aid digestion and assimilation of food. They have the same
beneficial effect as mint (Mentha spicata) and rosemary (Rosmarinus officinalis).
Laurel promotes the onset of menstruation. Diluted in neutral oil, the essential oil
is mainly used as an ointment to rub the muscles and painful joints. Added to bath
water decoction of the leaves soothes aching limbs.
6 Myrtle (Myrtus communis L.)
Taxonomy
The Myrtus word is derived from the Greek word “myron”, which is the fragrance:
aromatic plant (Coste 1937). Also it can be derived from the Arabic word
“Mayroune.” The Myrtus genus belongs to the Myrtaceae family. A native of the
Mediterranean basin, it includes about fifty species, some of which are also present
in Western Asia, South America and Australia (Zrira 2002).
Description
Evergreen shrub, 2–3 m high, Smooth, aromatic, evergreen; leaves opposite, very
close together, ovate-lanceolate acute, entire, leathery, petiole reduced, smooth and
shiny, not stipulated stipules. Flowers white, solitary, axillary, on long stalks, fra-
grant; calyx tube welded to the ovary, 5 spreading lobes; 5 petals; numerous sta-
mens; 1 style, single stigma; inferior ovary. Fruit fleshy barely Bay, ovoid, bluish
black, crowned by the calyx, few-seeded (Picture 9). The full maturity of the fruit is
reached in November. Flowering occurs in summer from June to August (Fennane

et al. 1987; Valdes et al. 1987).
Ecology and Distribution
Myrtle is the only representative of the family Myrtaceae in North Africa. It grows
in semi-arid bioclimatic, sub-humid and humid hot tempered variant on non-
calcareous soils.
This species is very common in the Rif centro-occidental, the area between
Larache, Tangier and Bab Taza in Mamora and Arbaa Sehoul. It is also present in
the region of Amezmiz (High Atlas of Marrakech) (Barbero et al. 1981).
Chemical Composition of Myrtle Essential Oil
The myrtle leaves contain tannin, resinous and bitter substances, and about 0.3%
essential oil. The extraction of EO occurs by stripping with steam. Performance will
vary depending on the scrub exposure level and the cutting period, technology and
mining equipment. It is generally of the order of 0.7%.
Myrtle essential oil has a fairly simple chemical composition with generally
α-pinene (8.0–31.4%, limonene, 1,8-cineole (20.5–31.0%), limonene (9.0–18.0%)
and myrtenyl acetate (10.0–20.0%) as major constituents (Assaf 1995; Chalchat
et al. 1998; Zrira et al. 2006).
The chemical composition of essential oils of myrtle is qualitatively stable.
However, the percentage of a compound may vary widely (Chalchat et al. 1998).
Uses of Myrtle
Myrtle is known since antiquities, as a symbol of love and peace. The uses of myrtle
in traditional medicine and modern pharmacopoeia are numerous. It is still widely
used to fight against diseases of the scalp, upper respiratory tract infections and
gastrointestinal diseases against hemorrhoids. The fruits of the myrtle green or dried
were used against bleeding; the juice of the berries is used as stomachic and diuretic
(Fournier 1948). Their powder was used to prepare a macerate against felons and
nail diseases and was also administered against seminal losses and sweat heart
(Bellakhdar et al. 1994). Myrtle flowers are used against heart disease and liver.
Their decoction is used to stop acute diarrhea, treatment of cough and rhinitis.
The myrtle essential oils pale yellow or amber colour with a camphor notes are
very spicy. These are used in perfumery. They mix well with bergamot, lavender or
lavender, rosemary, sage, hyssop and mugwort essential oil.
110 S. Zrira
Picture 10 Rosa spp.
This species is also used in formulations for flavouring sauces and meats mixed
with spicy essential oils such as bay leaf, cloves and cardamom. It can identify the
effect of ginger and positively changes the nutty flavour of nutmeg (Bellakhdar et al.
1994).
7 Rose (Rosa damascena & Rosa centifolia)
Taxonomy
The rose belongs to the genus Rosa. The plant is in the form of a shrub climbing
stems, erect or creeping, usually furnished with hairs or spines. The leaves are alter-
nate, deciduous or evergreen, flowers terminal, solitary or in corymbs. The numer-
ous carpels are inserted on a fleshy receptacle shaped urn. The genus Rosa has a
large number of species, which are, derived the countless farmed varieties or
hybrids. If more than five thousand roses are counted today, few of them have a
strong fragrance. This fragrance is also very variable according to the variety. Only
three roses are used for the isolation of their perfume: Rosa damascena Mill. cv.
trigintipetala Dieck, Rosa centifolia L and Rosa alba L (Zrira 2006).
(a) Rosa damascena Mill. variety Trigintipetala Dieck
This flower, called the ‘Damascus Red Rose’ and of unknown origin is cultivated
as a hybrid of R. gallica L. and R. canina L. Nowadays, it can be found growing in
the wild in regions of the Caucasus, Syria, Morocco and Andalusia. Rosa Damascena,
very fragrant, contains an amount of essential oil that can be extracted by steam
distillation. Being probably the most used in perfumery, it is grown extensively in
Bulgaria and, on a smaller scale, in Turkey. It is a flower with regular petals (about
thirty), long stamens with a piriform ovary, the elongate calyx. Yellow green stem is
almost thornless (Picture 10). Damascus rose must have a suitable climate, tempera-
ture and a moderate humidity, on airy low calcium ground. Flowering begins in late
April in the most exposed places and continues throughout the month of May (Zrira
2006).
(b) Rosa centifolia L.

Called the ‘Rose of May’, it’s cultivated extensively in the Grasse region (south
of France) and in North Africa. It contains a relatively large amount of essential oil
that cannot however be economically isolated by steam distillation. Small amounts
are used for the production of rose water, popular in the Mediterranean and Latin
regions. Most of the production of Rosa centifolia L. is used to extract roses con-
crete and absolute, by volatile solvents. From a botanical point of view, the rose of
May seems very close to Rosa damascena Mill (Zrira 2006).
(c) Rosa alba L. ou Rosa damascena Mill. var. Alba
The white peasant rose ‘contains much less essential oil than Rosa damascena’.
This oil is, in addition, of lesser quality. Stronger than the red rose of Damascus, the
peasant rose is cultivated in Bulgaria as a hedge around red roses and, at higher
altitudes where the damask rose blooms more (Zrira 2006).
Cultivation of Rose
Rosa damascena is cultivated in south of the High Atlas between the M’gouna chain
and the Saghro in the commonly known M’gouna and Dadès valleys. Outside this
area, the rose is found upstream of the two valleys and around Ouarzazate, Skoura
and Agdz. Dadès belongs to the pre-Saharan bioclimatic costs. The crop soils con-
sist of deep silt. Their alkaline pH varies between 7.4 and 7.8. The organic matter
content is low. The concentration of nitrogen varies from medium to low.
Planting is done from November to February in 40 cm trenches deep rooted that
receive plants, taken from tuft on old hedges.
The yield is about 0.8 kg of fresh roses per linear meter of hedge; it may reach
1.4 kg in good year for a production of 3500–5000 tons of fresh roses/year (Zrira
2006).
Extraction of Rose Essential Oil
Rose essential oil is obtained by hydro-distillation, which gives rose water and
essential oil. The essential oil is the most expensive and most popular material.
The method of distillation roses is different from those used for other plants.
Double distillation is practiced. The still must perform:
• Hydro-distillation;
• Reinjection of rose waters;
• Double distillation
25% of the essential oils is obtained in the first distillation, whereas 75% during
the rest of the second distillation. The essential oil content in the rose flower is quite
small, only about 2%.
112 S. Zrira
Extraction of Rose Concrete and Absolute
(a) Extraction of concrete

The extraction of concrete is processed by hexane as solvent. The raw material is
loaded into the extractor and is then exhausted by successive washings with a suit-
able solvent. After passing through a settler and a hub, a partial distillation is per-
formed. We obtain odorant molecules, waxes and pigments and the solvent to be
reused.
Depending on the nature of the plant we could obtain:
–– The resinoid obtained from dried matters (balms, gums and resins). It is used in
their state;
–– The concrete (treatment outcome of all fresh organs of the plant).
The solvent that dissolves the fragrant wax or miscella must be evaporated to
obtain rose concrete. Obtaining the concrete is done in two phases:
1. A concentration under atmospheric pressure in evaporators. This operation stops
at 60 °C in order not to alter the volatiles.
2. Final concentration under reduced pressure at low capacity condensers (50–
100 l). This operation is difficult and must be carried out at the lowest tempera-
ture possible, all traces of solvent must be removed. Each industry has its own
method for this phase.
(b) Concrete conversion to absolute
Although easy to carry and maintain thanks to the presence of waxy mass con-
crete are not directly used in perfume compositions. Thus, they must be transformed
into absolute (real cleared of waxes). The dewaxing is carried out by successive
washings of the concrete in the alcohol inactive polar solvent on waxes. Usually five
to six washes are made by kneading in threshing and precipitation of wax is obtained
at −20/−25 °C. Alcoholic or absolute solution is purified by filtration.
(c) Concrete and absolute yield
The concrete rose volatile solvent is extracted from Rosa centifolia and Rosa
damascena. The yield varies between 0.17% and 0.27% in concrete that gives 55%–
65% absolute.
Chemical Composition of Rose Extracts
Rosa damascena Mill. contains carboxylic acid, terpene, myrcene, and vitamin C.
–– Flavonoids such as kaempferol and quercetin glycosides were detected. (querce-
tin 3-O galactosid, quercetin 3-O-xyloside kaempferol glycosides, The kaemp-
ferol glycosides, along with the kaempferol aglycone, accounted for 80% of the
total compounds that were quantified, with kaempferol 3-O-glucoside being the
predominant component. The high flavonol content of approximately 16 g/kg on
a dry weight basis, rutin, quercitrin, myricetin, quercetin, apigenin, and
kaempferol.
–– Catechin, epicatechin, rutin
–– Antocyanins (like cyanidin-3-O-β-glucoside)
–– Proanthocyanidins kind of tannins
–– Phenolic acids (gallic acid etc., m-coumaric acid)
–– Major carotenoids as the β-carotene, lycopene, rubixanthin, zeaxanthin, lutein
–– Vitamin C (European Medicines Agency 2013).
The basic character of rose oil, mostly dependent upon citronellol and geraniol,
is further modified by nerol (5–11%) and farnesol (0.2–1.4%). Their contents are
slightly higher in village oils. Higher farnesol content leads to the establishment of
strong floral character and an overall improvement of body-note volume. Nerol not
only adds to the rosaceous character but also to its freshness. In those cases where
the geraniol content is low, however, the freshness of nerol manifests itself as
slightly citrusy. When geraniol content is high, the combination of citronellol, gera-
niol, farnesol, and nerol results in a strong, sweet, floral, fresh rosaceous character.
Other typical constituents of rose oil are geranyl acetate, nonanal, citronellyl for-
mate, citronellyl acetate, eugenol, methyl eugenol, cis-rose oxide, α-terpineol,
phenylethyl alcohol, and linalool. Damascenones and some sulfur compounds are
among the minor components. Stearoptenes (paraffins) are natural constituents of
rose oil (the major one being nonadecane) and due to their presence, rose oil solidi-
fies at room temperature and when refrigerated. The chemical composition and
physical properties of the rose absolute produced from rose concretes and the
extract, showed that rose absolute consists mainly of β-phenylethyl alcohol, citro-
nellol, geraniol, nerol, eugenol, methyl eugenol, geranyl acetate, benzyl alcohol,
nonadecane, nonadecene and farnesol. It was observed that the solid residue con-
tains mainly straight-chain saturated hydrocarbons with high molecular weight
(C-15-C-31) and the esters of carboxylic acids and a homologous series between
them exists. It was also concluded that the extract obtained by solvent extraction
from the residue of rose flower (Rosa damascena Mill.), which was subjected to
steam distillation is also, a valuable raw material (Aycı et al. 2005).
Properties and Uses
For centuries, the Damascus rose (Rosa damascena) has been considered a symbol
of beauty and love. The fragrance of the rose has been captured and preserved in the
form of rose water by an ancient method that can be traced back to biblical times in
the Middle East, and later to the Indian subcontinent. An Iranian doctor, Avicenna,
is credited with the discovery of the process for extracting rose water from rose pet-
als in the early eleventh century. Damascus roses were introduced into England
114 S. Zrira
during the reign of Henry VIII and were frequently displayed and scattered at wed-
dings and festivals. Nowadays, they are popular in craft projects and as potpourri
ingredients. They are used in wedding favors, gathered together in organza bags or
favor boxes, and they replace the traditional Avola sugared almonds to make per-
fumed keepsakes. They are also used to decorate festive tables and as hair decora-
tions when attached to hairpins.
The uses of the dried Damascus rose in beauty products are numerous. Soaking
Damascus rosebuds in water for 3 or 4 days releases a rose essence which can be
added to bath water or may be used to rinse hair after shampooing to leave the skin
and hair soft with the fragrance of roses. As the gentlest of all astringents, rose water
is often used as toner for fair and dry skin or as an anti-aging product in facial
creams. Damascus rose oil also has therapeutic properties that sooth the mind and
helps with depression, nervous tension and stress.
The flower buds and rose water are also used in herbal medicine. Indeed, every-
where in Morocco decoction of flower buds is used against stomach, and the infu-
sion of rose petals henna mixture is administered as a laxative. To remedy the
abdominal pain, rural women are preparing a dried flower decoction. Externally, the
pale pink petals are chewed in toothache. They are also mixed with the myrtle,
sweet clover, lavender and other plants to wet the Ghassul in the hair care. Rose
water is used, in the treatment of fevers, nausea states, migraines and sunstroke
(compresses on the head and forehead), otitis (ear drops) nervousness and anxiety
(a spoon to drink twice a day). Rose water (hydrosol) is also used in cosmetics in the
toilet water, cosmetics).
The rose odorous extracts (essential oil, concrete and absolute) have taken an
important place in perfumery. The Grasse region is the main production area. These
extracts are incoproprated in the formulation of high quality perfumes (Channel,
Yves Saint Laurent, Guerlain). Rose essence is used in many compositions, extracts,
tinctures, beauty milk, and ointments, cosmetic oils. Dried rose petals musk is used
for rose powder or the manufacture of scented sachets (Zrira 2006).
The Rose in Morocco
The perfumed rose (Rosa damascena) is a specific crop to Ouarzazate region. Its
cultivation is located in the Dadès Valley. Currently 4200 km linear of rose are under
the form of hedges or fences around agricultural plots, representing about 1000 ha
or 10% of the cultivated agricultural land. Roses’ farming is often compromised by
frost and cold that affect the flowering period and industrial quality of the rose. It
varies greatly from year to year with an average of 4000 tons of fresh roses per year.
Eleven hectares of Rose (Rosa centifolia) were cultivated in 1998 in the Tidass
region for the production of concrete and rose absolute.
The rose is usually harvested in the first half of May, in the morning at sunrise.
To produce 1 kg of dry rose, it takes 4–5 kg of fresh roses and we need 5000 kg of
fresh roses to obtain 1 kg of rose essence. Pale rose petals contain 0.03–0.04% of an
essential oil consisting of geraniol, nerol, citronellol, phenylethyl and aldehyde
(soluble in water from which the fragrance release in rosewater).
The rose is used for the production of several products:
• Dried flower blossoms,
• Rose water,
• Rose essence and
• Concrete and absolute
Different rose aromatic extracts are produced by BIOLAND and “Arômes du
Maroc” these are companies equipped with sophisticated modern processing units
at Kelaat M’gouna in the Dades Valley, in the province of Ouarzazate. The compa-
nies also have processing units in the city of Khemisset (BIOLAND) and Tiddas
“Les Arômes du Maroc”.
BIOLAND produces rose essence and concrete r from Rosa damascena while
“Les Arômes du Maroc” develop:
• Rose water, rose essence and concrete from Rosa damascena
• Concrete and absolute from Rosa centifolia
8 Rosemary (Rosmarinus officinalis L.)
Botanical Description
Rosemary is an ornamental, aromatic and medicinal plant known in France under

the name or marine rose (Fournier 1948) “Yazir” in the Moroccan vernacular or
“Iklil al JBAL” in classical Arabic. Rosmarinus genus belongs to the Lamiaceae
family and includes two species Rosmarinus officinalis L. and Rosmarinus
eriocalyx.
Rosemary is a very aromatic shrub, from 0.50 to over 1 m, evergreen, very
branching, very leafy. The leaves are evergreen, leathery, sessile, linear, rolled by
the edges, green and aggrieved above, white-tomentose beneath. The flowers are
pale blue or whitish, with peduncle very small. Small inflorescences axillary and
terminal racemes, calyx bell bi-labiate, powder, bare throat, full oval upper lip, the
lower 2 lanceolate lobes lipped corolla, with protruding tube to a larger helmet lip,
cleft, the lower 3 lobes, the wide and concave means 2 stamens salient fillets,
inserted in the groove of the corolla, fitted to the base of a small tooth. Fruits:
achenes trines, smooth (Picture 11). Flowering occurs between April and July
(Elamrani et al. 1994a, b).
116 S. Zrira
Picture 11 Rormarinus

officinalis L.
Rosemary (Rosmarinus officinalis L) is found throughout the Mediterranean, in the

scrub up to 1500 m altitude and gardens where it is used as an ornamental plant. It’s
mainly found in the Mediterranean (southern Europe, Asia Minor, Cyprus, North
Africa, the Canary Islands, Madeira).
In Morocco, it’s widespread in the Mediterranean part and especially in Eastern.
It’s found in the western Rif at the eastern part of the limestone ridge, and in the
eastern Morocco at the wooded steppes of Debdou El Atef and Jerada. It’s also
reported in the regions of Bouarfa and Midelt.
Rosemary is a species, typical of the Mediterranean region, requiring light and heat,
drought resistant. It acclimates particularly in the upper arid or areas under the hot
sub-humid to fresh. It prefers calcareous soils and well drained. This species lives
in a very dry sub-humid areas or regions.
Main Chemical Constituents of Rosemary
The main constituents of rosemary are:

• Essential oil (1.0–2.5%)
• Methylated flavones: genkwanine, luteolin, diosmetin, apigenin,

isoscutellaréine-7-O-glucoside
• Phenols acids: rosmarinic acid also called “tannin of Labiatae”
• Tricyclic phenolic Diterpenes: rosmaridiphenol, carnosolique acid (= carnosic
acid) rosmadial (carnosol = picrosalvine, rosmanol)
• Polysaccharides acid (6%)
• Triterpenes and steroids: oleanolic acid, ursolic acid derivatives (Hoffler et al.
1986)
The Essential Oil
Rosemary gives an essential oil (EO) pale yellow or light colored. It is a mobile
liquid with a strong odor, fresh, herbaceous, woody, slightly minty.
The chemical composition of rosemary EO is relatively constant. However, the
proportions of each component may vary significantly (Granger et al. 1973).
Chemical heterogeneity of rosemary populations was demonstrated. Thus chem-
ical varieties (or chemotype) were differentiated on the basis of their EO. Three
rosemary chemotypes were identified:
• 1.8-cineole chemotype, with 1,8-cineole as major constituent (Rosemary from
Italy, Tunisia and Morocco).
• Camphre- borneol chemotype to present mainly in Spain
• α-pinene and verbenone chemotype to (Corsica, France and Algeria)
In Morocco, three rosemary chemotypes were identified:
• 1.8 cineole chemotype;
• Camphor chemotype;
• α-pinene chemotype (Elamrani et al. 1997a, b).
The commercial rosemary essential oil (1,8-cineole chemotype) of Morocco is
characterized by the following components: 1,8- cineole: 54.4%, Camphor: 6.7%,
α-pinene: 5.5% and β-pinene: 4.6% (Zrira et al. 1997).
The Antioxidants
(a) Flavonoids
Many flavonoid molecules have been isolated and identified: diosmetin, luteolin
genkwanine the hispidulin and apigenin. Some of these flavonoids were isolated
under hétérosidique form (diosmoside, methoxy-6-lutéoloside and apigénol-7-
glucoside) and under glycosiliques form (Table 1) (Elamrani 1999).
(b) Phenolic acids:
Three phenolic acids were identified in rosemary: rosmarinic acid, chlorogenic
acid and caffeic acid (Table 2).
118 S. Zrira
Table 1 Chemical structures of flavonoids identified in R. officinalis L.

R'1
R'2
R1 O
R2
O
R3
R1 R2 R3 R'1 R'2
Luteoline OH OH OH OH OH
6-Metoxy-luteoline OH OCH3 OH OH OH
6-Metoxy-luteoline-7-methyl éther OCH3 OCH3 OH OH OH
Apigenine OH H OH OH H
Diosmetine OH H OH OH OCH3
Hispiduline OH OCH3 OH OH H
Salvigenine OCH3 OH H OCH3
OCH3
Genkwanine OCH3 OH OH H
6-Methoxy-Genkwanine H OH OH
OCH3 OCH3 H
Genkwanine-4'-methyl éther OH OH OCH3
5-Hydroxy-4'-7-dimethyl flavone OCH3 H OH H
OCH3 H OCH3
Diterpenes, Diterpene Lactones and Quinones:
Diterpene lactones were isolated from the leaves of R. officinalis. Some authors
have shown the presence of carnosolique acid and carnosol. Others have isolated the
ether of carnosolique acid and rosmanol and rosmadial and have reported the pres-
ence of royléanone and its derivatives in the roots (Tables 3 and 4) (Elamrani 1999;
Elamrani et al. 2000).
Triterpenes
The ursolic acid was isolated from the leaves of R. officinalis. Then other terpenic
acids were isolated: 19-α-acid hydroxyursolique, 2β-hydroxyoléanolique acid.
Triterpene hydrocarbons adjacent sturctures were also isolated: α-amyrin, β-amyrin
(Table 5).
Table 2 Phenolic acids in R. officinalis: rosmarinic acid, chlorogenic acid and caffeic acid
HO CH2 CH COOH
O CO CH CH OH
HO
OH
1'acide rosmarinique
H O CO CH CH OH
HOOC H
H OH
H H
HO OH
H OH
1'acide chlorogénique
HOOC CH CH OH
OH
1'acide caféïque
Table 3 Chemical structure of Diterpenes and diterpene lactones in R. officinalis
OH OH
OH O
O
O
CHO
OH
CHO
Rosmanol Rosmadial
OH
OH OH
O OH
HOOC
Carnosol Acide carnosolique

120 S. Zrira
Table 4 Chemical structure of diterpenique quinones in R. officinalis
7 H
6
R
R = H: Royleanone
R = OH: 7-Hydroxy-royleanone (Horminone)
R = -O-CO-CH3: 7-Acetoxy-royleanone
Table 5 Chemical structure of some triterpene in R.officinalis
H 3C R1
R2
CH3 CH3 R3
CH3
HO
R1 R2 R3
α-Amyrine H CH3 CH3
β-Amyrine CH3 H CH3
Acide ursolique H CH3 COOH
Acide oléanolique CH3 H COOH
Uses of Rosemary and Its Essential Oil
Because of their aromatic flavor, rosemary leaves are used for culinary purposes. In
the food industry, the dry leaves are used as food flavorings. Note that the Council
of Europe in his collection of natural and artificial flavorings, consider adding rose-
mary to foods as a spice, is permitted without limitations.
Rosemary is a tonic, general stimulant including adrenocortical glands. It is also
a pulmonary antiseptic. Rosemary is hypertensive, stomachic, antidiarrheal, due to
its diuretic and sudorific qualities. It is cholagogue and choleretic to the point that
the intravenous injection of the lyophilized extract prepared from the leaves in ani-
mals, double the bile secretion volume of the latter. Rosemary acts against the gall-
stones and stomach pains. In the intestine, it can be used in infections, colitis,
diarrhea and flatulence. Rosemary leaves after powdering can be used to relieve
stomach crises and strengthen this body. An infusion of the leaves has emmena-
gogue properties.
On the other hand external use of rosemary should not be underestimated. Thanks
to its healing and fungicidal properties, it can beneficially intervene to heal wounds,
burns, joint swelling and scabies.
Rosemary is widely used in folk traditions for its ability to keep the food against
degradation by microorganisms and especially against oxidation of animal and veg-
etable fats. Its antiseptic properties are widely used in some traditional techniques
of food preservation. Furthermore, the latest research showed that rosemary extracts
exhibit anti-mutagenic activity and a marked antioxidant power due to flavonoids,
polyphenols and phenolic acids it contains in significant amounts. The antioxidant
properties of rosemary are linked essentially to the presence of rosmarinic acid
(anti-inflammatory phenolic acid), rosmanol, rosmadial, carnasol, carnosolique acid
and rofficerone, separated from dry leaves by extraction with hexane.
Essential oils of rosemary are currently used in the perfumery industry, cosmet-
ics, soap and in the pharmaceutical industry. In perfumery, they are incorporated in
the composition of most colognes. Combined with other essential oils, especially
basil and patchouli, they provide fashionable perfumes. In the seventeenth century,
rosemary essential oil was used to prepare the famous Queen of Hungary water.
This essential oil is used in deterpenated state in the preparation of soaps, sham-
poos and deodorants. It finds its application in agriculture as insecticides and
disinfectants.
In pharmaceutical and therapeutic industry, rosemary essential oils are used as
raw material in the formulation of drugs. The rosemary essential oils rich in cineole
and pinene are used for medicinal purposes. Indeed, thanks to its anesthetic, anti-
septic and stimulant properties, pure cineole is commonly used in the treatment of
inflammation of the nose and throat as well as chronic bronchitis. They are used as
stimulating the scalp and as pest control.
References
Achhal A, Akabali A, Barbero M, Bernabid A, M’hirit A, Peyre C, Quezel P, Rivas-Martinez S

(1980) A propos de la valeur bioclimatique et dynamique de quelques essences forestières au
Maroc. Ecol Medit Marseilte 3:211–249
Adlouni A, Bennani H, El Messal M (2009) Argan-oil: From nutrition to Health. Congrès
International Plantes Aromatiques et Médicinales, Maroc 2009, Ifrane du 24 au 26 septembre
2009
Arctander (1960) Perfume and flavor materials of natural origin. Edition Elisabeth New Jersey,
pp 154–158, po 138–141
122 S. Zrira
Assaf N (1995) Valorisation du Myrtus communis var. italica dans la zone de Beni Idder, province
de Tétouan. Mémoire de troisième cycle pour l’obtention du diplôme d’Ingénieur des Eaux et
Forêts, option “Ecologie et gestion des ressources naturelles”. ENFI, Salé
Aycı F, Aydınlı M, Bozdemir A, Tuta M (2005) Gas chromatographic investigation of rose con-
crete, absolute and solid residue. Flav Fragr J 20(5):481–486
Barbero M, Fresneau M, Peyre C, Quezel P (1981) Sur la présence au maroc de Laurus azorica
(Seub.) Franco. Acte III Congr. OPTIMA. Anales jard. Bot Madrid 37 (2):467–472
Bellakhdar J (1978) Médecine traditionnelle et toxicologie ouest saharienne. Contribution à l’étude
de la pharmacopée marocaine. Edition: technique Nord-Africaine, Rabat. pp 55–71
Bellakhdar J, Baayaoui A, Kazdari A, Marechal J (1987) Herboristes et médecine traditionnelle
à Tissint, oasis présaharien du sud marocain (Province deTata) Al Birunya. Rev Mar Pharm
3(1):7–50
Bellakhdar J, Miki W, Honda G (1994) Herboristes et pharmacopée à Marrakech et à Salé (Maroc).
Simples et prescriptions. Al Biruniya, Rev. Mar. Pharm. Tome n°1. pp 26–27
Benjilali B, Richard H (1980) Étude de quelques peuplements d’armoise blanche du Maroc
(Artemisia herba-alba). Rivista Italiana EPPOS 62:69–74
Benjilali B, Saris J, Richard H (1982) Nouveaux chemotypes d’armoise blanche du Maroc
(Artemisia herba-alba Asso). Rivista Feraderno Agricolo Supplemento 2, atti delle giornata
internationali Saint Vincent, 26–27 April 1982
Benjilali B, Richard H, Liddle P (1984) Chemotypes d’armoise blanche du Maroc ‘Artemisia
herba-alba’. Rivista Feraderno Agricolo Supplemento 2, atti delle Giornata Internationali
Saint-Vincent 26–27 April 1984
Benjilali B, Hammoumi M, M’Hamedi A, Richard H (1985) Analyse en composantes principales
(ACP) et chimiotaxonomie de l’armoise blanche du Maroc (Artemisia herba-alba). Les cahiers
du C.E.A.M.S 2:12–21
Benjilali B, Richard H, Baritaux O (1986) Étude des huiles essentielles de deux espèces d’origan
du Maroc : Origanum compactum Benth et Origanum elongatum Emb. Et Maire Lebensem
Wiss U Technol 19:22–26
Boudy P (1951) Economie forestière nord-africaine, II: Monographie et traitement des essences
forestières, Larose, Paris, Fasc I: 382–414; III: Description forestière du Maroc, Stat. de rech.
forest. Maroc, Rabat, pp 190–219
Boulos L (1983) Médicinal plants of North-Africa. Référence Publications Inc. 218 st. Clair River
Drive, Box 344 Algonac, Michigan 48001
Bruneton J (1993) Pharmacognosie, Phytochimie, Plantes Médicinales. Tec. & Doc. Lavoisier,
Paris, p 915
Bruneton J (1999) Pharmacognosie, Phytochimie, Plantes Médicinales, Tec. & Doc. Lavoisier,
Paris, 2ème édition, pp 632–643
Chalchat JC, Garry RP, Michet A (1998) Essential Oils of Myrtle (Myrtus communis L.) of the
Mediterranean Littoral. J Essent Oil Res 10(6):1998
Chalchat JC et al (1994) Essential oil components in sawdust of cedrus atlantica from Morocco.
J Essen Oil Res 6(3):323–325
Charrouf Z, Guillaume D (1999) Ethnoeconomical, ethnomedical, and phytochemical study of
Argania spinosa (L.) Skeels. J. Ethnopharmacol 67:7–14
Charrouf Z, Guillaume D (2007) Huile d’argan une production devenue adulte. Les Technologies
de Laboratoire, n° 6 septembre-octobre 2007
Charrouf Z, Guillaume D (2008) Argan-oil: Occurrence, composition and impact on human health.
Eur J Lipid Sci Technol 110:632–636
Chimi H, Cillard J, Cillard P (1994) Autooxydation de l’huile d’Argan. Argania Spinosa L du
Maroc Sci Aliment 14:117–124
Costa J C, Capelo J, Jardim R, Sequeira M (2004) Catálogo Florístico do Arquipélago da Madeira.
Quercetea 6:187–200
Coste H (1937) Flore descriptive et illustrée de la France de la Corse et des contrées limitrophes
Tome 2, taxon n°1275. Paris: Librairies des Sciences et des Arts, p 75
Debouzie D, Mazih A (1999) Argan (Sapotaceae) trees as reservoirs for Mediterranean fruit fly
(Diptera: Tephritidae) in Morocco. Environ Entomol 28(1):53–60
Dupin A (1949) L’Arganier survivant de la flore tertiaire, providence du sud marocain, Elevage et
cultures
Duquenois P (1968) L’utilisation des huiles essentielles en pharmacie, leur normalisation et
l’europe du medicament Parf. Cosmet Sav 11:132–137
Emberger L (1938) Les arbres du Maroc et comment les reconnaître. La rose, éditeurs, París, 318 p
El Abid A (2006) Le cèdre de l’Atlas mémoire du temps, Le bois de cèdre, structure, propriétés et
utilisations, p 158
Elamrani A (1999) Les Huiles Essentielles des Romarins du Maroc (Rosmarinus officinalis et
Rosmarinus eriocalix): Rendements, Chimie, Chimiotaxonomie, Facteurs influençant le ren-
dement et la composition chimique des huiles essentielles. Thèse de Doctorat, Faculté des
Sciences Ben M’sik Casablanca
Elamrani A, Zrira S, Ismaili Alaoui M, Berrada M, Benjilali B (1994a) L’huile essentielle de roma-
rin du Maroc: effet de la période de récolte et du niveau de coupe sur les rendements et la
composition chimique. Rivisita Italiana EPPOS N° spéciale, 1994, pp 238–245
Elamrani A, Zrira S, Ismaili Alaoui M, Berrada M, B (1994b) Le romarin du Maroc (Rosmarinus
officinalis L.): Variabilité intraspécifique. Rivisita Italiana EPPOS N° spéciale, 1994,
pp 226–236
Elamrani A, Zrira S, Ismaili Alaoui M, Berrada M, Benjilali B (1997a) Effet de la fréquence de
coupe et de la période de récolte sur la production et la qualité de l’huile essentielle de romarin
(Rosmarinus officinalis L.) du Maroc. Rivisita Italiana EPPOS N° spéciale, 1997, pp 655–667
Elamrani A, Zrira S, Ismaili Alaoui M, Berrada M, Benjilali B (1997b) Caractérisation chimique
de l’huile essentielle de romarin (Rosmarinus officinalis L) du Maroc. Rivisita Italiana EPPOS
N° spéciale, 1997, pp 646–654
Elamrani A, Zrira S, Berrada M, Benjilali B (2000) A study of Moroccan Rosemary oils. J Essent
Oil Res 12:487–495
European Medicines Agency (2013) Assessment report on Rosa gallica L., Rosa centifolia L.,
Rosa damascena Mill., flos. Committee on Herbal Medicinal Products (HMPC), 15 December
2013 EMA/HMPC/137298/2013
Farines M, Charrouf Z, Soulier M (1981) The sterols of Argania spinosa seed oil. Phytochemistry
20:2038–2039
Fellat Zarrouk K, Smoughhen S, Maurin R (1987) A study of the pulp of the fruit of the Argan tree
(Argania spinosa) of Morocco. Fats and latex. Actes de l’Institut Agronomique et Veterinaire,
II. 7:3–4, 17–22
Fennane M (1987) La grande encyclopédie du Maroc: flore. Présentation du monde végétal GPE,
Cremona, pp 7–13
Fennane M, Ibn Tattou M, Banabid A, Bellakhdar J, Lawalle (1987) La grande encyclopédie du
Maroc. Flore et végétation – G.E.P. Crémona Italie
Fiorini C, Fouraste I, David B, Bessières JM (1997) Laboratoire de pharmacognosie, Faculté de
pharmacie, Toulouse, Fr. Flav Fragr J
Fournier P (1948) Le livre des plantes médicinales et vénéneuses de France. Edition Lechevalier.
Paris, 3. pp 335–336
Gef-Rif 3 (1998) Description des échantillons des produits de la distillation et développement
des opérations d’analyse de ces échantillons, présentation des résultats d’analyse. Projet de
production et de gestion participative des écosystèmes forestiers du Rif N° B 75040/I/94/02,
Chefchaouen
Hafizoglu H, Reunanen M (1993) Fett ISSN 0931–5985 CODEN FWTEEG, Vol. 95, no 8,
pp. 304–308 (49 ref.)
Imelouane B, El Bachiri A, Ankit M, Khedid K, Wathelet JP, Amhamdi H (2010) Essential oil
composition and antimicrobial activity of Artemisia herba-alba Asso grown in Morocco.
Banats J Biotechnol 1:48–55
124 S. Zrira
Hilali M, Charrouf Z, El Aziz SA, Hachimi L, Guillaume D (2005) Influence of origin and extrac-
tion method on Argan-oil physico-chemical characteristics and composition. J Agric Food
Chem 53:2081–2087
Hoffler C, Mortier F, Fleurintin J, Pelt JM, Guillemain J (1986) Composition chimique de
Rosmarinus officinalis L. (Lamiacées). Al Biruniya. Rev Mar Pharm 2(2):79–89
Husnia Marrif I, Ali BH, Hassan KM (1995) Some pharmacological studies on Artemisia herba-
alba (Asso.) in rabbits and mice. J Ethnopharmacol 49:51–55
Kenny L, De Zborowski I (2007) In Atlas de l’Arganier et de l’Arganeraie; IAV Hassan II: Rabat,
Morocco; p 190
Khallouki F, Younos C, Soulimani R, Oster T, Charrouf Z, Speigelhader B, Bartsch H, Owen RW
(2003) Consumption of Argan-oil (Morocco) with its unique profile of fatty acids, tocopherols,
squalene, sterols, and phenolic compounds should confer valuable cancer chemopreventive
effects. Eur J Cancer Prev 12(1):67–75
Mabberley DJ (1997) The plant book, a portable dictionary of the vascular plants, 2nd edn.
Cambridge University Press, Cambridge
Maurin R (1992) L’huile d’Argan Argania spinosa (L.) Skeels sapotaceae. Rev Fr Corps Gras
39:139–146
Matthaus B, Guillaume D, Gharby S, Haddad A, Harhar H, Charrouf Z (2010) Effect of processing
on the quality of edible Argan-oil. Food Chem 120:426–423
M’hirit O (1982) Etude écologique et forestière des cédraies du Rif marocain. Essai sur une
approche multidimensionnelle de la phytoécologie et de la productivité du cèdre (Cedrus atlan-
tica Manetti) Thèse es-Sciences Université d’Aix Marseille III
M’Hirit O (1994) Annales de la recherche forestière au Maroc, 27, vol 1. pp 5–15
M’Hirit O (2006) Le cèdre de l’atlas mémoire du temps, Taxonomie et répartition historique,
Répartition actuelle du cèdre de l’atlas p 13, 39–42
M’Hirit O, Benzyane M, Benchekroun F, El Yousfi SM, Bendaanoun M (1998) L’Arganier. Une
espèce fruitière-forestière à usages multiples; Mardaga: Sprimont, Belgium, p 150
Mohamed AE-HH, El-Sayed MA, Hegazy ME, Helaly SE, Esmail AM, Mohamed NS (2010)
Chemical constituents and biological activities of Artemisia herba-alba. Rec Nat Prod 4:1–25
Morton JF, Voss GL (1987) The Argan tree (Argania sideroxylon, Sapotaceae). Desert Sour Edible
Oil Econ Bot 41:221–233
Moufaddal (1983) Contribution à l’étude des possibilités d’extension du cèdre de l’Atlas (Cedrus
Atlatica Manetti) en dehors de son aire actuelle. Thèse pour l’obtention de Doctorat en Ecologie
générale et appliquée. IAV Hassan II, Maroc
Rahmani M (2005) Composition chimique de l’huile d’Argane “vierge”. Cahiers Agric 14:461–465
Rieuf P (1962) Les champignons de l’Arganier. Cah. Rech. Agron., n° 15
Roiron P (1979) Recherches sur les flores Plio-Quaternaires méditerranéennes: la mac-
roflore Pliocéne de Pichegru pres de Saint-Gilíes (Gard). Thése Doctorat 3éme cycle,
U.S.T.L. Montpellier
Rojas LB, Quideau S, Pardon P, Charrouf Z (2005) Colorimetric evaluation of phenolic content
and GC-MS characterization of phenolic composition of alimentary and cosmetic argan oil and
press cake. J Agric Food Chem 53:9122
Sayyah M, Saroukhani G, Peirovi A, Kamalinejad M (2001) Analgesic and anti-inflammatory
activity of the leaf essential oil of Laurus nobilis Linn
Service de Normalisation Industrielle (SNIMA) (2003) Huiles d’Arganes. Spécifications. Norme
Marocaine NM 08.5.090. SNIMA, Rabat, Morocco
Simic M, Kundakovic T, Kovacevic N (2003) Department of Pharmacognosy, Faculty of Pharmacy,
University of Belgrade, Vojvode Stepe 450, Belgrade 11221, Serbia and Montenegro
Valdes P, Tavavera S, Galiano EF (1987) Flora vascular de Andalucia occidental. Tome II. Ketres
Editoria S.A
Yaghmur A, Aserin A, Mizrahi Y, Nerd A, Garti N (1999) Argan-oil -in-water emulsions: prepara-
tion and stabilization. J Am Oil Chem Soc 76(1):15–18
Yaghmur A, Aserin A, Mizrahi Y, Nerd A, Garti N (2001) Evaluation of Argan-oil for deep fat fry-
ing. Lebensm-Wiss Technol 34:124–130
Zahar M, Reineccius G, Schirle-Keller J-P (2007) Identification of aroma compounds in food

grade Argan-oil. The 234th American Chemical Society meeting, August 19–23, Boston, MA,
USA
Zahar M, Reineccius G, Schirle-Keller J-P (2011) Argan-oil aroma: effect of kernels roasting and
oil extraction process. Actes du Premier Congrès International de l’Arganier, Agadir 15–17
Décembre 2011
Zrira S (2002) Etude des Plantes Aromatiques et Médicinales de la Région d’Aguelmame Sidi Ali-,
Rapport AMO, Projet PMF/FEM, PNUD
Zrira S (2006) La rose du Dadès: Culture et technologies d’extraction des essences de rose. Bulletin
Mensuel de Liaison et d’information Du PNTTA. Transfert De Technologie En Agriculture,
MADER/DERD N°146 Novembre 2006 PNTTA
Zrira S (2010a) Moroccan essential oils: Some important issues. IFEAT International Conference
in Marrakech, 26–30 Sept. 2010 ‘North African and Mediterranean Essential Oils and Aromas:
2010 Tales and Realities of our Industry – a new decade of challenges and opportunities’
Zrira S (2010b) Le cèdre de l’Atlas. 29èmes Journées Internationales Huiles essentielles et Extraits
Digne les Bains 24 & 25 Juin 2010
Zrira S (2011a) Les plantes aromatiques et médicinales du Maghreb. 30èmes Journées
Internationales Huiles essentielles et Extraits, DIGNE Les BAINS 14 & 15 Septembre 2011
Zrira S (2011b) The Value Chain of Aromatic & Medicinal Plants in the Maghreb. SIPAM, Tunisie,
2012
Zrira S, Elamrani A, Mejjati A, Belanger A, Ismaili Alaoui M, Benjlali B (1997) Rationalisation
de l’exploitation industrielle des plantes aromatiques et médicinales: cas du romarin: Plantes
Aromatiques et Médicinales et leurs Huiles Essentielles, Actes Edition pp 177–183 I.A.V
Hassan II, Maroc
Zrira S, Menut C, Bessiere JM, Benjilali B (2006) Propriétés biologiques de quelques huiles essen-
tielles marocaines. 25èmes Journées Internationales Huiles essentielles et Extraits, Digne les
Bains 14 & 15 septembre 2006
Prunus africana (Hook. f.) Kalkman (the
African Cherry)
Mélissa Rousseau, Claire Delvaux, Ezekiel Edward Mwakalukwa,

Lawrence Mbwambo, Marie Caroline Momo Solefack,
Harisoa Bako Ravaomanalina, Coert J. Geldenhuys,
Ntamwira Niranda Seintsheng, Nils Bourland, and Hans Beeckman
Abstract Prunus africana (Hook. f.) Kalkman, also known as Pygeum africanum,
is the only species of the Prunus genus found in Africa. The reputation of this tree
is due to the use of its bark extract in the treatment of benign prostatic hyperplasia
(enlarged prostate). The present review compiles the knowledge of the medicinal
uses, the extract composition and the threats to this overexploited tree but also pos-
sible solutions to sustainably produce its active compounds.
M. Rousseau (*) • C. Delvaux (*) • H. Beeckman

Wood Biology Service, Royal Museum for Central Africa,
Leuvensesteenweg 13, 3080 Tervuren, Belgium
e-mail: [email protected]; [email protected]
E.E. Mwakalukwa
Department of Forest Biology, Faculty of Forestry and Nature Conservation, Sokoine
University of Agriculture, P. O. Box 3010, Chuo Kikuu, Morogoro, Tanzania
L. Mbwambo
Tanzania Forestry Research Institute, P. O. Box 1854, Morogoro, Tanzania
M.C.M. Solefack
Department of Plant Biology, Faculty of Sciences, Dschang University,
B.P. 96, Dschang, Cameroon
H.B. Ravaomanalina
Department of Plant Biology and Ecology, Faculty of Sciences, Antananarivo University,
B.P 906 – 101, Antananarivo, Madagascar
C.J. Geldenhuys
Department of Forest and Wood Science, Stellenbosch University,
c/o Forestwood cc, P.O. Box 228, La Montagne 0184, South Africa
N.N. Seintsheng
Faculty of Sciences and Applied Sciences, Official University of Bukavu,
Bukavu, Democratic Republic of the Congo
N. Bourland
Wood Biology Service, Royal Museum for Central Africa,
Leuvensesteenweg 13, 3080 Tervuren, Belgium
Center for International Forestry Research, Situ Gede, Sindang Barang,
Bogor (Barat), 16115, Indonesia

DOI 10.1007/978-94-024-1120-1_6
128 M. Rousseau et al.
Keywords Prunus africana • African cherry • Bark • Benign prostatic hyperplasia

• Phytochemicals
1 Introduction
P. africana (Hook. f.) Kalkman is a multi-use species mainly known for its bark
whose extracts are used in the treatment of benign prostatic hyperplasia, a disorder
common in men over 50 years (Simons et al. 1998). In 1997, the annual trade of P.
africana bark and its derivatives was estimated at more than $220 million
(Cunningham and Mbenkum 1993).
Because the bark is harvested from the natural environment, the importance of its
international trade leads to the overexploitation of the natural populations (Hall
et al. 2000). This results in significant disturbances in the population dynamics
(Cunningham and Mbenkum 1993; Sunderland and Tako 1999; Ndam and Ewusi
2000; Stewart 2003).
Concerns about the durability of the bark harvest have led to the inclusion of P.
africana in Appendix-II of the Convention on International Trade in Endangered
Species (CITES) in 1995 (Cunningham and Mbenkum 1993). The aim was to regu-
late the trade and to reduce the pressure on natural populations. However, in addi-
tion to the impacts of its trade, habitat loss due to agriculture and climate change is
also threatening this species (Geldenhuys 1981; Mayaux et al. 2015). Moreover, the
increase of the population concerned by prostatic hyperplasia and the renewed inter-
est in herbal medicine make the bark of P. africana a product whose demand is
likely to increase while the supply is remaining stable or even decreasing
(Cunningham and Mbenkum 1993; Hall et al. 2000; Cunningham et al. 2014).
2 Geographical Distribution and Ecology
P. africana is a tree of the Rosaceae family, also known as Pygeum africanum or

African cherry. It is the only species of the genus that is indigenous to Africa
(Simons et al. 1998). This tree is restricted to high conservation value Afromontane
forest islands (White 1983) in the African continent (Angola, Cameroun,
Democratic Republic of the Congo, Ethiopia, Kenya, Malawi, Nigeria, Somalia,
South Africa, Sudan, Swaziland, Tanzania, Uganda, Zimbabwe) and outlying
islands (Bioko, Grand Comore, Madagascar, Sao Tome e Principe) (Kalkman
1965) (Fig. 1). Cameroon and Madagascar are the countries that contain the largest
populations of the species because of their relatively large areas of montane forests
(Stewart 2003).
P. africana is a light-demanding species growing within the secondary forests
(Stewart 2003; Kadu et al. 2013). This tree is most abundant in open areas along
Prunus africana (Hook. f.) Kalkman (the African Cherry) 129
Fig. 1 Geographical distribution of Prunus africana (Hook. f.) Kalkman in the African continent
and outlying islands (Source: http://www.ville-ge.ch/musinfo/bd/cjb/africa)
forest margins and in disturbed areas (Geldenhuys 1981; Ndam 1996) and occurs at
altitudes between 1000 and 2500 m (Cunningham and Mbenkum 1993; Sunderland
and Tako 1999). It can reach 30–40 m in height and a diameter of 1 m. Its bark
(Fig. 2) is light to dark reddish fissured (Stewart 2003).
Its evergreen canopy is medium to large with simple and alternate leaves (Stewart
2003) and small white flowers pollinated by insects (Farwig et al. 2008). The fruit
is a drupe that becomes purple colored at maturity (Stewart 2003) and it is dispersed
by birds and mammals such as red colobus and black and white colobus (Chapman
and Chapman 1999; Chapman et al. 2003; Fashing 2004). Birds can disperse the
relatively small seeds over distances of more than 300 km (White 1983). The differ-
ent organs (Fig. 3) (Sim 1907) and the bark emit a characteristic “cherry” odor of
the genus (Stewart 2003).
Three different zones can be distinguished based on flowering periodicity: the
equatorial zone where some individuals are flowering almost continuously and the
southern and northern zones where the flowering period seems to correspond with
Fig. 2 Prunus africana (Hook. f.) Kalkman bark from a tree located in Tshivanga at the entry of
the Kahuzi Biega National Park (Democratic Republic of the Congo)
Fig. 3 Flowering branch

(1) and fruit (2) of Prunus
africana (Hook. f.)
Kalkman (Adapted from
Sim 1907 [14])
cool and dry conditions (Hall et al. 2000). P. africana starts to produce seeds at
15–20 years, a relatively late age (Simons et al. 1998). The fruiting period, generally
associated with rainfall, seems to appear the 2–3 months after the flowering period
(Hall et al. 2000).
3 Medicinal Uses
P. africana is multi-use species known mainly for the beneficial effects of its bark
as treatment for prostate enlargement. Nevertheless, this species is also known for
its timber and its leaves, roots and bark as traditional medicines in Africa
(Cunningham and Mbenkum 1993; Kadu et al. 2012).
Before the 1960s, P. africana was only used for traditional purposes. In 1966, the
effectiveness of its bark to treat prostate hypertrophy (an enlargement of the pros-
tate) and Benign Prostatic Hyperplasia (BPH) was discovered (Debat 1966). Benign
prostatic hyperplasia is a non-cancerous enlargement of the prostate that commonly
affects men over 50 (Bombardelli and Morazzoni 1997; Simons et al. 1998). More
than 50% of men over the age of 60 have urinary symptoms that can be attributed to
BPH (Wilt and Ishani 1998). The number of side effects of other treatments of BPH
(drugs and surgery) make phytotherapy an increasingly popular option (Stewart
2001). Moreover, P. africana is well tolerated and costs less than most prescripted
medications (Wilt and Ishani 1998).
Though the mechanism of action of P. africana bark remains unclear, its efficacy
to treat BPH symptoms is believed to be due to at least three groups of compounds:
(a) phytosterols (β-sitosterol, β-sitostenone) having anti-inflammatory properties
(Carbin et al. 1990), (b) pentacyclic triterpenoids (oleanolic and ursolic acids) pro-
viding anti-edematous activity (Bombardelli and Morazzoni 1997) and (c) ferulic
acid esters (n-docosanol and n-tetracosanol) that lower blood levels of cholesterol,
from which testosterone is produced (Bombardelli and Morazzoni 1997). These
phytochemicals (Fig. 4) are believed to work synergistically to counteract the physi-
ological changes associated with the disease (Bassi et al. 1987; Bombardelli and
Morazzoni 1997; Simons et al. 1998).
Although the bark is the plant material commonly extracted, it has been found
that wood, flowers and fruits have similar therapeutic values (Debat 1974).
The extract from the plant material is used in a variety of pharmaceutical forms
such as tablets, capsules or cachets (Hall et al. 2000). To produce hundred 50-mg
capsules, 5 g of extract are enough (Simons et al. 1998). Usually, P. africana extract
is blended with other extracts known to have similar properties such as saw pal-
metto (Serenoa repens) (Lowe and Ku 1996).
a b
H
H
H
H
H H
H H
HO
O
c d
OH OH
H H
O O
H H
HO HO
H H
e
OH
f
OH
Fig. 4 Some of the phytochemicals present in Prunus africana extract involved in the treatment
of Benign Prostatic Hyperplasia (BPH). (a) β-sitosterol, (b) β-sitostenone, (c) oleanolic acid and
(d) ursolic acid, (e) n-docosanol and (f) n-tetracosanol
4 International Trade
Following the discovery of the use of P. africana in the treatment of BPH, pharma-
ceutical companies began hiring people to harvest bark for exportation to European
countries (Cunningham and Mbenkum 1993). The first countries to export from the
early 1970s were Cameroon, Madagascar, Democratic Republic of the Congo
(DRC), Kenya and Uganda (Cunningham et al. 1997). Cameroon, where the harvest
began in 1972 around Mount Cameroon, has the longest history in P. africana bark
trade (Stewart 2003).
In the 1990s, the annual quantity of dried bark or extract from P. africana
exploited to export to Europe ranged between 3200 and 4900 tons, making it the
Table 1 CITES national export quotas for Prunus africana (Source: https://cites.org/eng/
resources/quotas/export_quotas)
Country Quotas Unit Specimens
Burundi 0 –
Cameroon 1,082,879 kg Dry bark
Democratic Republic of the Congo 232 kg Dry bark
Kenya 0 –
Madagascar 0 –
Uganda 176,179 kg Dry bark
Fig. 5 Products derived from Prunus africana extracts
first of any African medicinal plant in international trade (Cunningham et al. 2002).
The global value of this international trade of products was estimated to be more
than USD 220 million per year with Cameroon providing approximately 60% of the
volume annually traded (Cunningham and Mbenkum 1993; Cunningham et al.
1997). However in the country, the price of the bark was less than USD 0.10 per kg
compared to the international value of USD 2 per kg (Cunningham et al. 1997). The
majority of the overall product value was taken by the pharmaceutical companies
(Clark and Sunderland 2004).
In 2012, due to the establishment of quotas per exporting country, the volume
traded was less than 1000 tons with Cameroon providing almost 75% of P. africana
bark at the market price of USD 6 per kg (Cunningham et al. 2014). Over the last 40
years, the changing demand and markets have led to strong fluctuations in the values
and volumes of bark traded (Ingram and Schure 2010).
Madagascar, Kenya and Burundi have a zero quota granted by CITES in 2016 so
the global supply is supplied by Cameroon, DRC and Uganda (Table 1). Most of the
bark harvested in Cameroon is exported to Italy and France while bark from DRC
is exported to France and Belgium (Cunningham et al. 2002). The herbal products
derived from P. africana (Fig. 5) are sold under its synonym, Pygeum africanum
(Stewart 2003). Originally only two brand-name products were produced with P.
africana extracts but now more than 40 brand-name products can be found on the
market of ten countries (Wei et al. 2005). Capsules containing bark extracts have
been marketed in Europe (mainly Austria, France, Italy and Switzerland) for over
40 years (Cunningham and Mbenkum 1993).
5 Threats
Unsustainable Harvest
Currently, all the P. africana bark found on the market is from wild harvest (Hall
et al. 2000). In theory, the harvest of its bark is supposed to be sustainable. Indeed
the tree has the faculty to regenerate its bark as long as the vascular cambium is not
damaged (Stewart 2003). Unfortunately, it is not the case in practice. The wild pop-
ulations of P. africana are highly vulnerable due to bark overexploitation (Hall et al.
2000; Kadu et al. 2012).
Studies showed that most trees are even debarked from upper branches or felled
and left to die to facilitate the access to the bark (Cunningham and Mbenkum 1993;
Stewart 2003). Even partially strip barking living trees expose them to ring-barking
and to insect attacks that can cause 50–90 % of post-harvest tree mortality (Ndam
and Ewusi 2000). In Equatorial Guinea, 68% of exploited P. africana trees for local
trade were experiencing death or canopy dieback (Sunderland and Tako 1999).
Early studies trying to evaluate the impacts of bark harvest showed that it affected
negatively the population structure, increased the mortality and decreased the fecun-
dity (Parrot and Parrot 1989; Ewusi et al. 1992; Cunningham and Mbenkum 1993;
Walter and Rakotonirina 1995). Death rates in commercially harvested wild popula-
tions of P. africana trees larger than 10 cm of diameter at breast height (dbh) can be
50–100 times higher than the natural mortality rate (Stewart 2003; Forboseh et al.
2011). Moreover, with the over-exploitation of large trees, there is a shift to trees
with increasingly smaller diameters at breast height (Cunningham and Mbenkum
1993). The impacts on the population are ever higher since the density of mature
trees is generally low in forests but also threaten ecosystem integrity (Ewusi et al.
1992; Hall et al. 2000).
The harvest of bark is geographically limited because of P. africana distribution
in a specific ecosystem. It also increases the pressure on small areas (Cunningham
et al. 2002). This harvest is still occurring even in our days, in Cameroon in areas with
a protected status (Mount Oku, Mount Cameroon and Mount Kupe) endangering
also other species (Cunningham and Mbenkum 1993). Moreover, in Madagascar,

the prescription that two seed trees per hectare should be left in the exploitation area
is not applied (Walter and Rakotonirina 1995; Hall et al. 2000). It is in Madagascar
and in Cameroon that P. africana bark is under the greatest threat (Cunningham and
Mbenkum 1993; Walter and Rakotonirina 1995). At an average P. africana density
of 5.5 trees ha−1 (Eben Ebai et al. 1992) and a bark yield of 55 kg per tree, the com-
mercial harvesting of 1923 tons of bark each year would affect over 6300 ha of
Afromontane forest annually (Cunningham and Mbenkum 1993).
With the world population aging, it is known that 88% of men in western coun-
tries will have the chance of developing histologic evidence of BPH (Cunningham
and Mbenkum 1993). The demand of P. africana bark is then likely to be increasing
which will strengthen the pressure on wild populations (Dawson et al. 2000; Hall
et al. 2000).
Loss of Habitat
P. africana is not only threatened by the unsustainable harvest of its bark but also by
the loss of its habitat. For example, the forest cover of P. africana habitat in Ethiopia
has been reduced from 35% in the beginning of the nineteenth century to less than
2.8% now (Mayaux et al. 2015). In Cameroon, the discovery of the potential of P.
africana have resulted in a densification of the human populations in the concerned
areas, causing a clearing of the forest for farming purposes (Cunningham and
Mbenkum 1993).
There exist different major threats to montane ecosystems that are P. africana
habitat: clearing for agriculture, timber extraction, browsing and trampling by
livestock, and fire (Cunningham et al. 2008). Forest disturbances have impacted on
species populations that can show an episodic natural regeneration as a result
(Cunningham et al. 1997).
Considering the range of the species overall, the destruction of montane forests
is probably the major factor affecting its conservation status (Hall et al. 2000).
Climate Change
Climate change is another threat to P. africana populations that has to be consid-

ered. Sensitivity of Afromontane forest plants to small changes in temperature and
rainfall can have high impacts on plants viability (Conway 2009). In South Africa,
a study showed that 47% of P. africana standing stems of more than 10 cm diameter
at breast height were dead due to increasing aridity in the Southern Cape region
(Geldenhuys 1981). Moreover, climate changes can influence regeneration at the
edges of the species’ natural range (Geldenhuys 1981).
The countries that are more likely to be negatively affected by changes in

ountain ecosystem climates are Tanzania, Madagascar, Cameroon, Democratic
m
Republic of the Congo and Uganda (Ingram et al. 2015).
6 Protection Status
In 1995, growing concerns about the sustainability of P. africana harvest led to the
species to be listed in CITES Appendix-II (Cunningham and Mbenkum 1993).
In 2005, Cunningham summarized in the CITES Significant Trade Review evi-
dences of the destructive and highly unsustainable harvest of the bark (Ewusi et al.
1992; Sunderland and Tako 1999; Stewart 2001; Nkeng et al. 2009; Ingram 2014).
This report was discussed at the CITES meeting in Lima in 2006. In 2007, the
European Union banned the importation of P. africana. But because of the pressure
from the private sector and from the Cameroon government, the ban was lifted in
2011 and quotas have been established for the main producing countries
(Cunningham et al. 2014). This lift was mainly based on the “Management Plan for
Prunus”, a report published by different research organizations including the Center
for International Forestry Research (CIFOR) (Ingram et al. 2009; Ingram 2014).
However, there were many reservations about the poor quality of the report even
among the authors (Cunningham et al. 2014).
Besides its status for CITES, P. africana is also listed in the Red List of
International Union for Conservation of Nature (IUCN, www.iucnredlist.org) as
a vulnerable species. Despite those designations, P. africana exported volumes
are still making it Africa’s most exported medicinal plant species (Cunningham
et al. 2002).
7 Bark Harvesting and Composition
As mentioned previously, P. africana is among the few African tree species that
exhibit complete bark regrowth and is even able to withstand complete bark removal
(Stewart 2003). This ability offered the potential for sustainable harvesting of P.
africana bark (Cunningham and Mbenkum 1993). The method currently presented
as sustainable for bark removal is the two-quarter method (Fig. 6), with the proce-
dure described by Ndam and Yogo (1999). Only the trees with a dbh of more than
30 cm can be debarked. The trees with a dbh below 50 cm should be debarked with
two strips in opposite sides, each no wider than 1/4 of the tree circumference, from
1 m above the ground up to the first branch. From those with a dbh of 50 cm or
more, four strips should be removed, each no wider than 1/8 of the tree circumfer-
ence. Moreover each portion debarked on the tree should be separated from the next
one by an untouched section of the bark of the same width. Lateral roots with a
minimum diameter of 20 cm on trees with a dbh above 50 cm can also be debarked.
Fig. 6 Two-quarter method for Prunus africana (Hook. f.) Kalman bark harvest (bark in dark
brown) for trees with a diameter at breast height (dbh) of less than 50 cm (a) and for trees with a
dbh of more than 50 cm (b) from 1.30 m height to the first branch
The trees should completely recover from the first debarking before subsequent
debarking (Ndibi and Kay 1997).
The recommendations for a sustainable harvest of P. africana bark also include
the time between the harvest of the first 50% of the bark and the harvest of the last
part. The minimum time of rotation has been found to be 7–8 years (Ingram 2014).
The problem for the harvesters is that the bark yield decreases with an increase of
the rotation time. From a 5-year rotation to a 6-year rotation, the bark yield can be
reduced by more than 20% (Cunningham et al. 2014).
In practice, the use of this harvest technique shows that the tree responds differ-
ently depending on the site. In moist sites, bark regrowth occurs but in dry sites, bark
does not recover (Cunningham et al. 2014). This leads to wood-borer and fungal
attack on the debarked trees (Cunningham et al. 2002). Insects’ attacks also occur in
lower altitude sites, even on healthy P. africana trees (Cunningham et al. 2014).
There is a tendency to harvest P. africana bark during dry or low rainfall periods
(Cunningham and Mbenkum 1993; Walter and Rakotonirina 1995). Using the
method described previously, between 15 and 100 kg (depending on the size of the
tree) can be obtained from a tree at each harvest (Tsobeng et al. 2008). To produce
5 kg of extract 1 ton of dry bark (that corresponds approximatively to the double of
fresh bark) is needed to be harvested from mature trees (Cunningham et al. 1997).
Differences in genetic and chemical constituents of the bark have been found
across the distribution range of P.africana that may reflect environmental differ-
ences within the range of a species (Kadu et al. 2012). A study in Cameroun,
Democratic Republic of the Congo and Madagascar has already shown that the
chemical composition of the bark depends on its origin (Martinelli et al. 1986).
Madagascar, a region genetically distinct from the African continent, seems to have
populations with very high chemicals constituents studied by Kadu et al. (2012).
8 Perspectives for a Sustainable Use
For a sustainable use of P. africana, it is important to focus on reducing the impacts

of the current techniques of harvest on the populations’ survival but also on looking
for alternatives to wild harvest. A lot of research is absolutely necessary to underpin
a sound harvest aiming at a sustained production of bark.
Bark, Cambium and Wood Anatomy
The macroscopic phenomenon of P. africana bark recovery has been studied and it
is known that after debarking, P. africana rapidly produces new bark on the wound
surface (Cunningham and Mbenkum 1993; Vermeulen and Geldenhuys 2004;
Geldenhuys et al. 2007). No information exists of this phenomenon at the micro-
scopic level. However, to understand how P. africana can regenerate its bark, it is
essential to study the tissues and the anatomical feature modification due to the
injury. Delvaux et al. (2013) proposed the size of the conducting phloem as a key-
factor for bark regeneration. Research should make it clear how, when and where
new cambium is generated and how fast new tissues are being formed.
Inventory and Monitoring
To assess the state of the P. africana populations and the impacts of the current
techniques on their survival, a standardized and accepted method of sampling needs
to be adopted. The National Management Plan for P. africana in Cameroon (Ingram
et al. 2009) included inventories that used different methods, with very different
results, even for the same locations (Cunningham et al. 2014). In their study,
Morrison et al. (2008) found that grid-based systematic designs were the more effi-
cient and practical method for sampling.
It is also important to assure that bark is not harvested from protected areas. A
recent project carried out by ITTO-CITES has developed a DNA tracking technol-
ogy for P. africana bark (Darren 2016). With this technique, it is possible to identify
the origin of the harvested bark and if it was from authorized harvest zones.
9 Cultivation
Unlike P. africana, other tree species from which the bark is commercially har-
vested (cinnamon, cork oak, chestnut, etc.) have made the transition from wild har-
vest to production in agroforestry or plantation systems (Lubbe and Verpoort 2011).
In Cameroon, several attempts have started to cultivate P. africana more than 20
years ago (Cunningham and Mbenkum 1993). Small-scale farmers began producing
the species in agroforestry systems (Cunningham and Mbenkum 1993; Tchoundjeu
et al. 2002). In terms of industrial cultivation, only 9 ha of pure plantation have been
established by the Cameroon Development Corporation (Ndam and Tonye 2004).
The annual P. africana plantation targets of 2 ha set in 1986 and 5 ha set in 1992
(Ndibi and Kay 1997) was far too small to have a meaningful impact (Hall et al.
2000). Attempts at cultivation are also underway in Kenya (Dawson et al. 2000;
Dawson and Powell 1999).
There are different cultivation practices to consider that can be found in Ingram
et al. (2015). For example, Tchoundjeu et al. (2002) found that vegetative propaga-
tion is suitable for P. africana, giving other options to domestication. The harvest of
leaves is another alternative to bark harvest, since both plant material have the same
pharmaceutical properties (Debat 1974).
For P. africana conservation, it is important to have regional approaches because
of the genetic variation across the range but even at the country level. Indeed, differ-
ences have been found in populations in Madagascar and in Cameroon (Dawson
and Powell 1999). It would be especially interesting to do research on the potential
of the species in agroforestry systems.
10 Conclusion
The growing interest for P. africana is strengthening the threats the species is fac-
ing. It is therefore necessary to protect wild populations and find other ways to
produce P. africana bark in order to sustainably supply the pharmacological indus-
try with its valuable extract.
References
Bassi P, Artibani W, De Luca V, Al E (1987) Estratto standardizzato di Pygeum africanum nel trat-
tamento dell’ipertrofia prostatica benigna. Studio clinico controllato versus placebo. Minerva
Urol Nefrol 39:45–50
Bombardelli E, Morazzoni P (1997) Prunus africana (Hook.f.) Kalkm. Fitoterapia 68:205–218
Carbin B-E, Larsson B, Lindahl O (1990) Treatment of benign prostatic hyperplasia with phytos-
terols. Brit J Urol 66:639–641
Chapman CA, Chapman LJ (1999) Implications of Small-scale variation in ecological conditions

for the diet and density of red colobus monkeys. Primates 40(1):215–231
Chapman CA, Chapman LJ, Rode KD, Hauckand EM, McDowell LR (2003) Variation in the
nutritional value of primate foods: Among trees, time periods, and areas. Int J Primatol
24:317–333
CIFOR : Bogor, Indonesia. http://www.cifor.org/publications/pdf_files/WPapers/
WP153Sunderland.pdf. Accessed 29 June 2015
Clark LE, Sunderland TC (2004) The key non-timber forest products of Central Africa: state of the
knowledge. SD Publication Series Technical Paper No. 122. L. E. Clark and T. C. Sunderland,
U.S. Agency for International Development, Office of Sustainable Development, Bureau for
Africa: 1999
Conway G (2009) The science of climate change in Africa: impacts and adaptation. Discussion
paper No 1. Grantham Institute for Climate Change. London Imperial College, UK
Cunningham AB, Mbenkum FT (1993) Sustainability of harvesting Prunus africana bark in
Cameroon: a medicinal plant in international trade. People and plants Working Paper, vol 2,
pp 1–28
Cunningham M, Cunningham AB, Schippmann U (1997) Trade in Prunus africana and the imple-
mentation of CITES. German Federal Agency for Nature Conservation, Bonn. Available via
https://www.researchgate.net/publication/276028274_Trade_in_Prunus_africana_and_the_
Implementation_of_CITES. Accessed 24 July 2015
Cunningham AB, Ayuk E, Franzel S, Duguma B, Asanga C (2002) An economic evaluation of medic-
inal tree cultivation: Prunus africana in Cameroon. In: People and plants Working Paper No.
10. UNESCO, Paris. Available via http://unesdoc.unesco.org/images/0013/001330/133098e.
pdf. Accessed 29 June 2015
Cunningham AB et al (2008) Sustainable trade and management of forest products and services in
the COMESA region. Cifor. Available via http://www.cifor.org/publications/pdf_files/Books/
BCunningham0801.pdf. Accessed 27 July 2015
Cunningham A, Avana Tientcheu M-L, Anoncho V, Nkuinkeu R, Sunderland T (2014) Power,
profits and policy: a reality check on the Prunus africana bark trade. In Working Paper 153. 10
Darren T (2016) A pilot study of Prunus africana shows that DNA tracking technology can help
safeguard supply chains from illegality. ITTO Trop For Update 25(1):16–19
Dawson IK, Powell W (1999) Genetic variation in the Afromontane tree Prunus africana, an
endangered medicinal species. Mol Ecol 8:151–156
Dawson I, Were J, Lengkeek A (2000) Conservation of Prunus africana, an over-exploited African
medicinal tree. For Genet Resour FAO 28:27–33
Debat J (1966) Br. App. 15.893/66, 10 June 1966
Debat J (1974) Prunus africana extract. US Patent 3,856,946, 24 Dec 1974
Delvaux C, Sinsin B, Van Damme P, Beeckman H (2013) Size of conducting phloem: The « key »
factor for bark recovery of 12 tropical medicinal tree species. Flora 208:111–117
Eben Ebai S, Ewusi BN, Asanga CA, Nkongo JBN (1992) An evaluation of the quantity and
distribution of Pygeum africanum on the slopes of Mount Cameroon. Divisional Service of
Forestry, Limbe
Ewusi BN, Eben-Ebai S, Asanga CA, Nkongo JBN (1992) An evaluation of the quantity and distri-
bution of Pygeum africanum on the slopes of Mount Cameroon. Unpublished Report prepared
for Plantecam-Medicam
Farwig N, Carsten B, Bohning-Gaese K (2008) Human disturbance reduces genetic diversity of an
endangered tropical tree, Prunus africana (Rosaceae). Conserv Genet 9:317–326
Fashing PJ (2004) Mortality trends in the African cherry (Prunus africana) and the implications for
colobus monkeys (Colobus guereza) in Kakamega Forest, Kenya. Biol Conserv 120:449–459
Forboseh PF, Sunderland TCH, Comiskey JA, Balinga M (2011) Tree population dynamics of three
altitudinal vegetation communities on Mount Cameroon (1989–2004). J Mt Sci 8:495–504
Geldenhuys CJ (1981) Prunus africana in the Bloukrans River Gorge, Southern Cape. S A Fores
J 118:61–66
Geldenhuys CJ, Syampungani S, Meke GS, Vermeulen WJ (2007) Response of different species
to bark harvesting for traditional medicine in Southern Africa. In: Multiple use management of
natural forests and woodlands: policy refinement and scientific progress. Department of Water
Affairs and Forestry, Indigenous Forest Management, Pretoria, pp 55–62
Hall JB, O’Brien EM, Sinclair LF (2000) Prunus africana: a monograph. School of Agricultural
and Forest Sciences. Publication No 18 University of Wales, Bango
Ingram V (2014) Profitability and value chain analysis of Prunus africana commercialisation from
Mount Cameroon, Cameroon, P.-. GIZ (ed). Coopération Germano – Camerounaise. Ministère
des Forêts et de la Faune (MINFOF). Ministère de l’Environnement et de la Protection de
la Nature et du Développement durable (MINEP). Deutsche Gesellschaft für Internationale
Zusammenarbeit (GIZ) GmbH: The Netherlands and Cameroon, p 100
Ingram V, Schure J (2010) Review of non timber forest products (NTFPs) in Central Africa:
Cameroon. CIFOR, Yaoundé
Ingram V, Awono A, Schure J, Ndam N (2009) Guidance for a national Prunus africana man-
agement plan. Cameroon. Center for International Forestry Research, Food and Agriculture
Organization, Cameroon, p 158
Ingram V, Van Loo J, Vinceti B et al (2015) Ensuring the future of the pygeum tree (Prunus afri-
cana). Factsheet (p 8). LEI Wageningen UR
Kadu CAC, Parich A, Schueler S, Konrad H, Muluvi GM, Eyog-Matig O, Muchugi A, Williams
VL, Ramamonjisoa L, Kapinga C et al (2012) Bioactive constituents in Prunus africana: geo-
graphical variation throughout Africa and associations with environmental and genetic param-
eters. Phytochemistry 83:70–78
Kadu CAC, Konrad H, Schueler S, Muluvi GM, Eyog-Matig O, Muchugi A, Williams VL,
Ramamonjisoa L, Kapinga C, Foahom B, Katsvanga C, Hafashimana D, Obama C, Geburek
T (2013) Divergent pattern of nuclear genetic diversity across the range of the Afromontane
Prunus africana mirrors variable climate of African highlands. Ann Bot 111:47–60
Kalkman C (1965) The Old World species of Prunus sub-genus Laurocerasus including those
formerly referred to as Pygeum. Blumea 13:1–115
Lowe FC, Ku JC (1996) Phytotherapy in treatment of benign prostatic hyperplasia: a critical
review. Urology 48:12–20
Lubbe A, Verpoort R (2011) Cultivation of medicinal and aromatic plants for specialty industrial
materials. J Ind Crops Prod 34:785–801
Martinelli EM, Seraglia R, Pifferi G (1986) Characterization of Pygeum africanum bark extracts
by HRGC with computer assistance. J High Resolut Chromatogr Chromatogr Comm
9:106–110
Mayaux P, Pekel J-F, Desclée B, Donnay F et al (2015) State and evolution of the African rainfor-
ests between 1990 and 2010. Philos Trans R Soc Lond Ser B Biol Sci 368(1625)
Morrison LW, Smith DR, Young CC, Nichols DW (2008) Evaluating sampling designs by com-
puter simulation: a case study with the Missouri bladderpod. Popul Ecol 50(4):417–425
Ndam N. (1996) Recruitment patterns of Prunus africana (Hook f.) Kalkman on Mount Cameroon:
a case study at Mapanja. In: A strategy for the conservation of Prunus africana on Mount
Cameroon. Technical Papers and Workshop Proceedings
Ndam N, Ewusi B (2000) Case study: income from Prunus africana. In: Price MF, Butt N (eds)
Forests in sustainable mountain development: a state of knowledge report for 2000. CAB
International, Wallingford, pp 306–309
Ndam N, Tonye MM (2004) Prunus africana on Mount Cameroon: a case study of the production-
to consumption systems. In: Sunderland T, Ndoye O (eds) Forest products, livelihoods and
conservation. Case studies of non-timber forest product systems, vol 2 – Africa, pp 37–52
Ndam N, Yogo C (1999) A review of NFORKEMBA exploitation of Prunus africana in
Cameroun
Ndibi BP, Kay EJ (1997) The regulatory framework for the exploitation of medicinal plants in
Cameroon: the case of Prunus africana on Mount Cameroon. Biodivers Conserv 6:1409–1412
Nkeng PF, Ingram V, Awono A, Tientcheu MLA (2009) Assessment of Prunus africana bark
exploitation methods and sustainable exploitation in the South west, North-West and
Adamaoua regions of Cameroon, in Project GCP/RAF/408/EC « Mobilisation et Renforcement
des Capacités des Petites et Moyennes Entreprises impliquées dans les Filières des Produits
Forestiers Non Ligneux en Afrique Centrale », CIFOR, Editor. FAO-CIFORSNV- World
Agroforestry Center-COMIFAC, Yaounde
Parrott J, Parrott H (1989) Report on the conservation of Prunus (Pygeum africanum) in Cameroon.
Unpublished Report prepared for the International Council for Bird Preservation (Now Birdlife
International)
Sim TR (1907) Forests and forest flora of the colony of the Cape of Good Hope. Taylor &
Henderson, Aberdeen
Simons AJ, Dawson IK, Duguma B, Tchoundjeu Z (1998) Passing problems: prostate and Prunus.
HerbalGram 43:49–53
Stewart KM (2001) The commercial bark harvest of the African cherry (Prunus africana) on
Mount Oku, Cameroun: effects of traditional uses and population’s dynamics. Dissertation,
Florida international University
Stewart KM (2003) The African cherry (Prunus africana): can lessons be learned from an over-
exploited medicinal tree? J Ethnopharmacol 89:3–13
Sunderland T, Tako CT (1999) The exploitation of Prunus africana on the island of Bioko,
Equatorial Guinea. Report for the People and Plants Initiative, WWF-Germany and the IUCN/
SSC Medicinal Plant Specialist Group, Bonn, p 13
Tchoundjeu Z, Avana ML, Leakey RRB, Simons AJ et al (2002) Vegetative propagation of
Prunus africana: effects of rooting medium, auxin concentrations and leaf area. Agrofor Syst
54:183–192
Tsobeng A, Degrande A, Tientcheu M-LA, Tchoundjeu Z, Tabuna H (2008) Cultivation and sus-
tainable bark collection of Prunus africana. In: Technical Note. ICRAF, Yaounde, p 15
Vermeulen WJ, Geldenhuys CJ (2004) Experimental protocols and lessons learnt from strip har-
vesting of bark for medicinal use in the southern Cape forests. In: FRP-DFIP Project R8305
Report, Wild Resources Limited, UK (unpublished)
Walter S, Rakotonirina J-CR (1995) L’exploitation de Prunus africana à Madagascar. PCDI
Zahamena et la Direction des Eaux et Forêts, Antanarivo/Fénérive-Est, p 23
Wei JT, Clahoun E, Jacobsen SJ (2005) Urologic diseases in America project: benign prostatic
hyperplasia. J Urol 173(4):1256–1261
White F (1983) Long-distance dispersal and the origins of the Afromontane flora. Sonderbände
Naturwissenschaftliche vereins Hamburg 7:87–116
Wilt TJ, Ishani A (1998) Pygeum africanum for benign prostatic hyperplasia. Cochrane database
of systematic reviews 1
Bird Plum; Berchemia discolor (Klotzsch)
Hemsl.: A Review of Its Ethnobotany,
Phytochemistry and Pharmacology
Ahmad Cheikhyoussef and Alfred Maroyi
Abstract Bird plum (Berchemia discolor) is a shrub or tree distributed in the

Arabian Peninsula and Africa characterized by edible fruits and its wood makes
charcoal, building material, beehives, furniture and crafts. Berchemia discolor is
also used as a dye, fodder, ornamental tree and as herbal medicine to treat several
human and animal diseases and ailments. The objective of this study was to collate
the fragmented information on the ethnobotanical uses, phytochemical and pharma-
cological properties of the Bird plum throughout its geographical range in the
African continent so as to highlight research gaps and provide a foundation for
further investigations on the plant species. The utilization of Berchemia discolor as
a food plant, for various other uses including its medicinal applications forms the
basis of the current demand for the plant species in Africa. Research on Berchemia
discolor over the past decade on its health promoting properties have greatly con-
tributed to the increased consumption of the species as a food plant and herbal medi-
cine. Therefore, further studies are required to improve our knowledge about its
phytochemistry, mechanisms of action, efficacy, toxicity and clinical relevance of
the plant species.
Keywords Bird plum • Berchemia discolor • Africa • Food • Herbal medicine •

Livelihood needs
A. Cheikhyoussef (*)
Science and Technology Division, Multidisciplinary Research Centre (MRC),
University of Namibia, Private Bag 13301, Windhoek, Namibia
A. Maroyi
Medicinal Plants and Economic Development (MPED) Research Centre,
Department of Botany, University of Fort Hare, Private Bag X1314,
Alice 5700, South Africa

DOI 10.1007/978-94-024-1120-1_7
144 A. Cheikhyoussef and A. Maroyi
1 Taxonomy and Geographic Distribution
The genus Berchemia Neck. ex DC. is named after M. Berchem, a seventeenth cen-
tury French botanist. The specific epithet discolor means the species has two differ-
ent colours (Palmer and Pitman 1972) referring to the fact that the upper and the
lower leaf surfaces are of different colours, that is, green and silver grey respec-
tively. Berchemia is a member of the Rhamnaceae, characterized by 51 genera and
over 600 species, distributed throughout the warm countries of the world (Palmer
and Pitman 1972). It is native to Angola, Botswana, Eritrea, Ethiopia, Kenya,
Madagascar, Malawi, Mozambique, Namibia, Somalia, South Africa, Swaziland,
Tanzania, Uganda, Yemen, Zambia and Zimbabwe. Berchemia discolor is com-
monly known as bird plum; other vernacular names are given in Table 1.
Berchemia discolor is a semi-deciduous shrub or tree (Fig. 1a), usually between
3 and 20 m in height (Palmer and Pitman 1972). The trunk is a straight bole with
rough, dark grey bark (Fig. 1b) that flakes longitudinally, with a dense and rounded
crown. The branches are conspicuously covered by lenticelles. Branchlets are gla-
brous to densely pubescent with short, spreading whitish hairs. The leaves (Fig. 1c)
are alternate or sub-opposite, entirely or obscurely crenate, shiny above, dull and
dark green below with raised veins on the lower side. Flowers are small, solitary and
thick, while fruits are datelike, yellow to pale orange with a single stone in sweet,
edible flesh.
2 Traditional Uses
Berchemia discolor is one of the most valuable non-timber forest product in sub-
Saharan Africa. The bark, fruit juice, leaves and roots are used to treat numerous
health complaints including abdominal pains, abscesses, flu and cold, general body
pains, infertility, malaria, menorrghagia, nose bleeding, skin itching, toothache and
livestock diseases (Table 2).
The bark of Berchemia discolor is used as an alluring love charm in South Africa
(Hutchings et al. 1996) and as poultices (Hutchings et al. 1996; van Wyk and
Gericke 2007). Berchemia discolor is also used to produce alcoholic drink, animal
food, gum or resin, human food, live fence, ornaments, tannin or dye, timber,
tobacco pipes and windbreak (Table 2). The fruits are marketed mainly by women
and children to generate income in Ethiopia, Namibia and Zimbabwe (Mukamuri
and Kozanayi 1999; Feyssa et al. 2012a; Cheikhyoussef and Embashu 2013). Bille
et al. (2013) reported on three value added products from Berchemia discolor fruits
in Namibia, these products are: juice, jam and jelly which were scored through a
sensory evaluation model with 3.2 ± 0.28 for juice, 2.95 ± 0.29 for jam and 2.31 ±
0.27 for jelly and they were significantly different (<0.05) from the other two fruits
used in their study.
Bird Plum; Berchemia discolor (Klotzsch) Hemsl.: A Review of Its Ethnobotany… 145
Table 1 Vernacular names of Berchemia discolor

Vernacular name(s) Country Reference(s)
Bird plum English, South Palmer and Pitman (1972), Mabogo (1990),
Africa Hutchings et al. (1996), and van Wyk and Gericke
(2007)
Swaziland Long (2005)
English, Ganduri et al. (2014)
Zimbabwe
Brown ivory English, South Hutchings et al. (1996) and van Wyk and Gericke
Africa (2007)
Bruinivoor Afrikaans, South Hutchings et al. (1996) and van Wyk and Gericke
Africa (2007)
Deen Somali, Kenya Gachathi et al. (1994)
Eembe Namibia Barrion et al. (2001) and Bille et al.(2013)
Jajab Orma, Kenya Gachathi et al. (1994)
Jajabho Malakote, Kenya Gachathi et al. (1994)
Jejeba Amharic, Ethiopia Dejen and Merene (2013)
Matatya Shona, Zimbabwe Wild et al. (1972)
Mgandu Gogo, Tanzania Hines and Eckman (1993)
Mkuni Nyamwezi, Hines and Eckman (1993)
Tanzania
Mmupudu Botswana Ohiokpehai (2003)
Mnago Tanzania Hines and Eckman (1993)
Monyee Botswana Kayombo et al. (2014)
Motsentsila Tswana, South van Wyk and Gericke (2007)
Africa
Mountain date English, South Palmer and Pitman (1972) and Hutchings et al.
Africa (1996)
Mozinzila South Africa Palmer and Pitman (1972)
Muchukwo Kenya Kipkorir and Kareithi (2012)
Mugaramhanga Shona, Zimbabwe Wild et al. (1972)
Muhukhuma Venda, South Palmer and Pitman (1972)
Africa
Mukhukhuma Venda, South Mabogo (1990)
Africa
Mujajabho Malakote, Kenya Gachathi et al. (1994)
Munhacha Shona, Zimbabwe Wild et al. (1972)
Munie Venda, South Mabogo (1990)
Africa
Munyi Shona, Zimbabwe Wild et al. (1972)
Munyii Tonga, Zimbabwe Wild et al. (1972)
Munyiyi Shona, Zimbabwe Wild et al. (1972)
Mutsintsila Tswana, South Palmer and Pitman (1972)
Africa
Muzinzila Lozi, Zambia Sorensen (1993)
(continued)
Table 1 (continued)
Vernacular name(s) Country Reference(s)
Muwe Tsonga, South Liengme (1981)
Africa
Mwii Tonga, Zimbabwe Mukamuri and Kozanayi (1999)
Nyahumbu Pogoro, Tanzania Hines and Eckman (1993)
Nyii Hlengwe, Wild et al. (1972)
Zimbabwe
Shona, Zimbabwe Mukamuri and Kozanayi (1999)
Nyiri Tsonga, South Liengme (1981)
Africa
Nyiyi Tsonga, South Palmer and Pitman (1972) and Liengme (1981)
Africa
Okoo Sandawi, Tanzania Hines and Eckman (1993)
Omuve Herero, Namibia Palmer and Pitman (1972)
Omuye Oshiwambo, Cheikhyoussef and Embashu (2013)
Namibia
Pink ivory English, Ganduri et al. (2014)
Zimbabwe
Ubalatsheni likulu Zulu, South Africa Palmer and Pitman (1972)
Ubalatsheni omkulu Zulu, South Africa Hutchings et al. (1996)
Umadlozane Zulu, South Africa Hutchings et al. (1996)
Umhlungulo Zulu, South Africa Hutchings et al. (1996)
UmMumu Zulu, South Africa Palmer and Pitman (1972)
Umncaga Ndebele, Wild et al. (1972)
Zimbabwe
Umnyi Zimbabwe Ganduri et al. (2014)
Umnyiyi Ndebele, Wild et al. (1972)
Zimbabwe
Umumu Zulu, South Africa Hutchings et al. (1996)
Uvuku Zulu, South Africa Hutchings et al. (1996)
Swaziland Long (2005)
Voëlpruim Afrikaans, South Carruthers (2000)
Africa
Wild almond English, South Palmer and Pitman (1972) and Hutchings et al.
Africa (1996)
Wild almond English, Tanzania Hines and Eckman (1993)
Wilde dadel Afrikaans, South Palmer and Pitman (1972) and Hutchings et al.
Africa (1996)
3 Phytochemistry
The seed oil content from Berchemia discolor give a yield of 11% as reported by
Sunder and Sino (1992). The unsaturated fatty acids (70%) (UNSAT), saturated
fatty acids (30%) (SAT) with ω-6/ω−3 ratio of 17 (Bombarda et al. 2010).
Polygalacturonase was extracted from ripe Berchemia discolor fruits in Zimbabwe
Fig. 1 Berchemia discolor in Namibia at the main campus of the University of Namibia, Windhoek
(a), barks on trunk (b) leaves and fruit (c) (Taken by author)
with optimum pH ranging from 4.5 to 5 and optimum temperature from 25 to 37 °C

(Muchuweti et al. 2005).
The qualitative phytochemical analysis revealed the presence of tannins, flavo-
noids, saponins, total phenols, steroids, phlobatanins, terpenoids, cardiac glycosides
and anthraquinones (Cheikhyoussef et al. 2010). The total phenolic content (TPC)
in fruit extracts ranged between 33.98 ± 0.05 and 40.03 ± 0.04 mg gallic acid equiv-
alent (GAE)/g. Total flavonoid content (TC) in bark extracts ranged from 3.829 ±
0.04 to 5.473 ± 0.08 mg equivalents to quercetin (QE)/g. Fruit extract had the high-
est content of saponins and the lowest content of tannins, meanwhile, bark extracts
showed the highest content of tannins and the lowest content of saponins
(Cheikhyoussef 2014). The presence of these phytochemicals in Berchemia dis-
color extracts is probably responsible for the medicinal efficacy of this plant in
Namibian traditional healing system (Cheikhyoussef et al. 2011). Bark extracts
exhibited the highest reducing power (508.36 %) followed by leaves (388.83%)
then fruit (377%). The DPPH radical scavenging activity in bark extracts was very
high (30.27%) compared to leaves and fruits extracts at 23.65% and 13.09% respec-
tively (Cheikhyoussef 2014).
Table 2 Ethnobotanical uses of Berchemia discolor

Plant part(s) used and Country
Use preparation practised Reference(s)
Medicinal uses
Abdominal pains Roots extract used against Zimbabwe Maroyi (2011)
abdominal pains medicine
Abscesses Leaves used against Ethiopia Desta (1993)
abscesses
Bleeding gums Fruit juice used to treat South Africa van Wyk and Gericke
bleeding gums (2007)
Flu and cold Leaves used against flu Namibia Cheikhyoussef and
and cold Embashu (2013)
General body Body washed with root Zimbabwe Gelfand et al. (1985)
pains infusion as remedy for
general body pains
Infertility Bark and fruits used for South Africa Mabogo (1990)
infertility
Love charm Bark used as an alluring South Africa Hutchings et al. (1996)
Malaria Stem bark boiled with Tanzania Chin et al. (2006)
roots of Cordia crenata
Delile and Tamarindus
indica L. as remedy for
malaria
Menorrghagia Smoke from burning roots South Africa Arnold and Gulumian
used to treat menorrghagia (1984)
Nose bleeding Bark used against nose Namibia Cheikhyoussef and
bleeding Embashu (2013)
Poultices Boiled leaves used as South Africa Hutchings et al. (1996) and
poultices van Wyk and Gericke
(2007)
Skin itching Bark used against skin Namibia Cheikhyoussef and
itching Embashu (2013)
Toothache Bark used for toothache Madagascar Jenkins (1987)
Veterinary herbal Bark used as veterinary Namibia Cheikhyoussef and
medicine herbal medicine Embashu (2013)
Other ethnobotanical uses
Alcohol Alcoholic drink distilled Namibia, South Barrion et al. (2001), van
from fruits Africa Wyk and Gericke (2007)
and Cheikhyoussef and
Embashu (2013)
Animal food Fruit and leaves used as Ethiopia; Kenya; Palmer and Pitman (1972),
fodder for baboons, birds, South Africa; de Groot and Hall (1989),
camels, cattle, giraffe, Tanzania Hines and Eckman (1993)
goats and monkeys and Feyssa et al. (2012a)
Apiculture Bees attracted to the Kenya, Tanzania Hines and Eckman (1993)
flowers; people hang and de Groot and Hall 1989
beehives in the trees and
tree trunks used for
making beehives
(continued)
Table 2 (continued)
Plant part(s) used and Country
Use preparation practised Reference(s)
Gum or resin Heartwood produces gum Tanzania Hines and Eckman (1993)
and resin
Human food Dried fruit flesh pounded Ethiopia; Kenya, Palmer and Pitman (1972),
to make a meal which, Nambia, South Liengme (1981), de Groot
when mixed with seeds of Africa, and Hall (1989), Mabogo
Eleusine coracana Asch. Swaziland; (1990), Hines and Eckman
& Gr. and Pennisitum Tanzania, (1993), Mukamuri and
typhoides Stapf. & Zimbabwe Kozanayi (1999), Long
C.E. Hubb. is kneeded (2005), Feyssa et al.
into a biscuit dough and (2012b) and Cheikhyoussef
baked or steamed. and Embashu (2013)
Beverage similar to tea
made from leaves, and
fruits added to porridge
Income generation Fruits marketed mainly by Ethiopia, Mukamuri and Kozanayi
women and children to Namibia, (1999), Feyssa et al.
generate income Zimbabwe (2012a) and Cheikhyoussef
and Embashu (2013)
Live fence Species conserved at farm Ethiopia Feyssa et al. (2012a)
boarders, live fences and
enclosed pasture (kalo)
areas
Ornaments Seeds used by Namibia Loeb et al. (1956) and
Kwanyamas of Namibia Palmer and Pitman (1972)
to decorate hair of women
Tannin or dye Brown and purple dyes Botswana; Palmer and Pitman (1972),
come from the wood and Namibia; South Mabogo (1990), Hines and
bark, these provide the Africa; Eckman (1993), Mukamuri
colouring for basket Swaziland; and Kozanayi (1999), Long
fibres. Mauvish-brown Tanzania; (2005), van Wyk and
colour given to fishing Zimbabwe Gericke (2007), Shumba
nets by boiling them in et al. (2010) and Ganduri
water mixed with bark of et al. (2014)
Lannea stuhlmannii
Timber Wood excellent for Kenya; South Palmer and Pitman (1972),
axe-handles, building Africa; Liengme (1981), de Groot
material, charcoal, crafts, Swaziland; and Hall (1989), Hines and
furniture, firewood, knife Tanzania; Eckman (1993), Mabogo
handles, knobkerries, Zimbabwe (1990), Mukamuri and
mortar, poles, pestles and Kozanayi (2001), Long
hair combs (2005), van Wyk and
Gericke (2007), Kipkorir
and Kareithi (2012) and
Ganduri et al. (2014)
Tobacco pipes Fashioned from wood Botswana Palmer and Pitman (1972)
Windbreaks Spreading branches and Tanzania Hines and Eckman (1993)
heavy rounded crown
makes Berchemia discolor
an effective shade tree and
act as windbreak
The fruit pulp has high sugar content (30%), and contains minerals (K, Ca, Mg, P)
and vitamin C (65 mg/100 g) and seeds taste like walnuts (Venter and Venter 1996).
The concentration of proteins and carbohydrates in Berchemia discolor were
18.21–19.59%, 16.72–16.89% respectively. There was a significant interactive
effect (p < 0.05) between different land uses and nutrient contents for all the param-
eters, dry matter (DM), moisture, crude fiber (CF) and crude protein (CP) (Feyssa
et al. 2012a). They further concluded that land use had significant effect on the
nutritional content of Berchemia discolor indicating that land use should be consid-
ered as one factor of production and domestication of the species in Ethiopia (Feyssa
et al. 2012a). The nutrients contents of fruits significantly varied for all variables
analysed except for copper (Cu) and condensed tannin (CT). Phosphorus, calcium,
zinc, magnesium, manganese are relatively higher in fruits collected from transhu-
mance land use than settled farmers land use, while iron is relatively higher from
fruits collected from settled farmers land use (Feyssa et al. 2012a). Vitamin-A con-
tent of Berchemia discolor significantly varied across land uses (p < 0.05) with
higher mean value from samples collected from transhumance land use systems.
There was no significant variation in vitamin-C content across land uses (p > 0.05).
Therefore, land use is a factor to be considered in domestication of the species.
Moreover, the high vitamin C indicates the potential that consumption of the fruits
can enhance metallic nutrients absorption such as iron. These two vitamins are also
among the critical vitamins focused by current human nutrition security (Feyssa
et al. 2012a).
4 Pharmacology
Chin et al. (2006) isolated five new prenylated flavonoids (Fig. 2) from the root bark
of Berchemia discolor, collected in Tanzania, along with ten known compounds, by
bioactivity-guided fractionation. The isolated compound exhibited cytotoxic activ-
ity when evaluated against three human cancer cell lines (Lu1, LNCaP, and MCF-7)
(Chin et al. 2006).
The structures of the known compounds were identified by physical and spectro-
scopic data measurement ([α] D, CD, 1H NMR, 13C NMR, 2D NMR, and MS) and
by comparing these with the data obtained from published studies, such as nitidulin,
heminitidulan, leiocarpin, leiocin, leiocinol, and nitidulan (Fig. 3) (van Heerden
et al. 1981), amorphigenin and dabinol (Abe et al. 1985), 3-hydroxy-4′-O-
methylglabridin (Kinoshita et al. 1996) and 4′-hydroxycabenegrin A-I (Fig. 4)
(Silva et al. 1997).
Four compounds namely, (3,S)-5,2′,3′-trihydroxy-4′-methoxy-3″-methyl-3″-(4-
methylpent-3-enyl)-pyrano[7,8] isoflavanone, nitidulin, amorphigenin and dabinol
exhibited cytotoxic activity (ED50 ≤ 5 μg/mL) for one or more cancer cell lines.
Among them, nitidulin inhibited the growth only of the LNCaP cell line (49–52%),
propagated within the intraperitoneal site, at all doses tested. The compound was
not active against any of the lines propagated subcutaneously (Chin et al. 2006).
Fig. 2 Five newly reported prenylated flavonoids from the root bark of Berchemia discolor col-
lected in Tanzania (Adapted from Chin et al. 2006)
O O
O O O
O
H
O
O O O
O O
O
Nitidulin Nitidulan Heminitidulan
O
O
O O
O
O O
H
O O O
O
H
Leiocin Leiocinol
Fig. 3 Prenylated flavonoids from Berchemia discolor (Group I)

O H
O O O
O H O
O
O
H O O
O O
O O
O
Amorphigenin Dabinol
OH
H3C
O O
O OH
H
HO OCH3
H
OH O O
O
3-hydroxy-4′-O-methylglabridin Cabenegrin AI
Fig. 4 Prenylated flavonoids from Berchemia discolor (Group II)
Prenylated flavonoids have been identified in 37 of plant genera only and prenyl-
ation usually renders flavonoids with improved bioactivities (Yang et al. 2015). The
mechanism of action is prenylation increases the lipophilicity of flavonoids, which
results in a higher affinity to biological membranes and a better interaction with
target proteins (Xu et al. 2012).
The antimicrobial activities of Berchemia discolor plant parts extracts were eval-
uated with well diffusion method (Perez et al. 1990). Ethanolic, methanolic and
aqueous extracts of Berchemia discolor showed the strongest effects towards the
indicator strains such as Staphylococcus aureus ATCC 25923 (inhibition diameter 9
± 0.86 mm), Bacillus subtilis ATCC 11774 (13.5 ± 2.12 mm), Alcaligenes faecalis
ATCC 8750 (11 ± 0.57 mm) Clostridium difficile ATCC 9689 (4.25 ± 1.06 mm),
Escherichia coli ATCC 25922 (13 ± 1.52 mm) (Cheikhyoussef 2014) (Fig. 5).
Lactobacillus plantarum ATCC 8014 has not been affected by any extracts of
Berchemia discolor. This could be explained by the fact that Lactobacillus ssp. have
the ability to metabolize the phenolic compounds (Gyawali and Ibrahim 2012) and
these compounds also serve as oxygen scavengers, which could have increased the
growth of certain probiotic bacteria (Alberto et al. 2001).
Fig. 5 Antimicrobial activities of three extracts of Berchemia discolor using well diffusion method
toward Staphylococcus aureus ATCC 25923 (a) and Alcaligenes faecalis ATCC 8750 (b)
(Cheikhyoussef 2014)
5 Conclusion
Berchemia discolor is an important tree species characterized by edible fruits which

are of social and economic value throughout its distributional range in African
countries. Berchemia discolor is of high nutritional value but its consumption and
use is not well promoted by the cultural production systems. Berchemia discolor is
nutritionally rich in terms of major food nutrients such as carbohydrates, proteins,
minerals, crude lipids, macro and micronutrients. Therefore, it can significantly
contribute to human nutrition and ecosystem services to enhance human wellbeing.
It provides livelihood needs in terms of nutrition, income generation, fuel wood,
timber, fodder and medicine. Berchemia discolor extracts showed a broad inhibitory
spectrum against food-borne bacteria and yeast. Scientifically based evidence on the
health benefits of different parts of Berchemia discolor extracts may provide a far-
ther value addition to this important fruit tree in the African continent.
References
Abe F, Donnelly DMX, Moretti C, Polonsky J (1985) Isoflavonoid constituents from Dalbergia
monetaria. Phytochemistry 24:1071–1176
Alberto MR, Farías ME, de Nadra MCM (2001) Effect of gallic acid and catechin on Lactobacillus
hilgardii 5w growth and metabolism of organic compounds. J Agric Food Chem 49:4359–4363
Arnold HJ, Gulumian M (1984) Pharmacopoeia of traditional medicine in Venda. J Ethnopharmacol
12:35–74
Barrion S, Keya EL, Ngwira TN (2001) Country-wine making from Eembe fruit (Berchemia dis-
colour) of Namibia. J Food Technol 6
Bille PG, Shikongo-Nambabi M, Cheikhyoussef A (2013) Value addition and processed products
of three indigenous fruits in Namibia. Afr J Food Agric Nutr Dev 13(1):7192–7212
Bombarda I, Zongo C, McGill CR, Doumenq P, Fogliani B (2010) Fatty acids profile of Alphitonia
neocaledonica and Grevillea exul var. rubiginosa seed oils, occurrence of an ω5 series. J Am
Oil Chem Soc 87:981–986
Carruthers V (2000) Die natuurlewe van Suider-Afrika. Struik Uitgewes, Kaapstad
Cheikhyoussef A (2014) Ethnobotanical, phytochemical, antioxidant and antimicrobial activi-
ties of Namibian indigenous fruits and vegetables. Paper presented at the INSPIRE 2014;
Sustainable Future for Green Medicine Symposium, 26–27 March 2014, Nelson Mandela
Metropolitan University, George, South Africa
Cheikhyoussef A, Embashu W (2013) Ethnobotanical knowledge on indigenous fruits in
Ohangwena and Oshikoto regions in Northern Namibia. J Ethnobiol Ethnomed 9:34
Cheikhyoussef A, Shapi M, Matengu K, Mu Ashekele H (2011) Ethnobotanical study of indig-
enous knowledge on medicinal plant use by traditional healers in Oshikoto region, Namibia.
J Ethnobiol Ethnomed 7:10
Cheikhyoussef A, Naomab E, Potgieter S, Kahaka G, Raidron C, Mu Ashekele H (2010)
Phytochemical properties of a Namibian indigenous plant; Eembe (Berchemia discolor). In:
Proceedings of the first national research symposium, Windhoek, Namibia, pp 313–321
Chin Y-W, Mdee LK, Mbwambo ZH, Mi Q, Chai H-B, Cragg GM, Swanson SM, Kinghorn AD
(2006) Prenylated flavonoids from the root bark of Berchemia discolor, a Tanzanian medicinal
plant. Nat Prod 69:1649–1652
De Groot P, Hall D (1989) Back to grass roots. New Sci 121:44–47
Dejen A, Merene Y (2013) Ecology and field biology of the sorghum chafer, Pachnoda interrupta
(Olivier) (Coleoptera: Scarabaeidae) in Ethiopia. J Entomol Nematol 5:64–69
Desta B (1993) Ethiopian traditional herbal drugs. Part 2: antimicrobial activity of 63 medicinal
plants. J Ethnopharmacol 39:129–139
Feyssa DH, Njoka JT, Asfaw Z, Nyangito MM (2012a) Nutritional value of Berchemia discolour:
a potential to food and nutrition security of households. J Biol Sci 12:263–271
Feyssa DH, Njoka JT, Asfaw Z, Nyangito MM (2012b) Comparative analysis of indigenous
knowledge on use and management of wild edible plants: the case of Central East Shewa of
Ethiopia. Ethnobot Res Appl 10:287–304
Gachathi FN, Johansson SG, Alakoski-Johansson GM (1994) A check-list of indigenous trees and
shrubs of Bura, Tana river district, Kenya with Malakote, Orma and Somali names. J East Afr
Nat Hist 83:117–141
Ganduri L, Nyathi JA, Nkiwane L, Nyoni AB (2014) Potential of natural dye processing in
Matabeleland South Province: case study of Masendu Ward, Bulilima-Mangwe District
Zimbabwe. Zimb J Sci Technol Spec Issue MS 11:1–7
Gelfand M, Drummond RB, Mavi S, Ndemera B (1985) The traditional medical practitioner in
Zimbabwe: his principles of practice and pharmacopoeia. Mambo Press, Gweru
Gyawali R, Ibrahim SA (2012) Impact of plant derivatives on the growth of foodborne pathogens
and the functionality of probiotics. Appl Microbiol Biotechnol 95:29–45
Hines DA, Eckman K (1993) Indigenous multipurpose trees of Tanzania: uses and economic ben-
efits for people. Cultural Survival Canada and Development Services Foundation of Tanzania
Hutchings A, Scott AH, Lewis G, Cunningham AB (1996) Zulu medicinal plants: an inventory.
University of Natal Press, Pietermaritzburg
Jenkins MD (1987) Madagascar: an environmental profile. IUCN, Gland
Kayombo B, Tsheko R, Semetsa S, Malepa D (2014) Documentation of indigenous knowledge
and best-bet practices on the use of animals and plants for sustainable natural resources man-
agement in Botswana. Botswana J Agric Appl Sci 10(1):3–10
Kinoshita T, Kajiyama K, Hiraga Y, Takahashi K, Tamura Y, Mizutani K (1996) Isoflavan deriva-
tives from Glycyrrhiza glabra (licorice). Heterocycles 43:581–588
Kipkorir D, Kareithi J (2012) Human and natural factors in the deterioration of indigenous irriga-
tion Furrows in Marakwet, Kenya. Int J Humanit Soc Sci 2:113–128
Liengme CA (1981) Plants used by the Tsonga people of Gazankulu. Bothalia 13:501–518
Loeb EM, Koch C, Loeb EMK (1956) Kuanyama Ambo magic. Part 6. Medicinal, cosmetical and
charm flora and fauna. J Am Folk 69:147–174
Long C Swaziland’s flora (2005) siSwati names and uses. http://www.sntc.org.sz/flora/clbotalpha.
asp?l=C&pg=10
Mabogo DEN (1990) The ethnobotany of the Vhavenda. MSc. Thesis. University of Pretoria.
Pretoria, South Africa.
Maroyi A (2011) Ethnobotanical study of medicinal plants used by people in Nhema communal
area, Zimbabwe. J Ethnopharmacol 136:347–354
Muchuweti M, Moyo E, Mushipe S (2005) Some properties of the polygalacturonase from four
Zimbabwean wild fruits (Uapaca kirkiana, Zizyphus mauritiana, Tamarindus indica and
Berchemia discolor) fruits. Food Chem 90:655–661
Mukamuri B, Kozanayi W (1999) Institutions surrounding the use of marketed bark products: the
case of Berchemia discolour, Warburgia salutaris and Adansonia digitata. IES Working Paper
17. University of Zimbabwe, Harare.
Mukamuri BB, Kozanayi W (2001) Commercialization and institutional arrangements involving
tree species harvested for bark by small-holder farmers in Zimbabwe. IES Working Paper 18.
University of Zimbabwe, Harare.
Ohiokpehai O (2003) Promoting the nutritional goodness of traditional food products. Pak J Nutr
2:267–270
Palmer E, Pitman N (1972) Trees of southern Africa, covering all known indigenous species in
the republic of South Africa, South-West Africa, Botswana, Lesotho and Swaziland. Balkema,
Cape Town.
Perez C, Pauli M, Bazerque P (1990) An antibiotic assay by agar well diffusion method. Acta
Biologica Medica Exp 15:113–115
Shumba E, Chidumayo E, Gumbo D, Kambole C, Chishaleshale M (2010) Biodiversity of plants.
In: Chidumayo G (ed) The dry forests and woodlands of Africa: managing for products and
services. Earthscan, London
Silva GL, de Abreu Matos FJ, Silveira ER (1997) 4′-dehydroxycabenegrin A-I from roots of
Harpalyce brasiliana. Phytochemistry 46:1059–1062
Sorensen C (1993) Controls and sanctions over the use of forest products in the Kafue river basin
of Zambia. Rural Development Forestry Network, ODI Forest Policy & Engineering Group,
London
Sundar Rao K, Sino D (1992) Fatty acid composition of 20 lesser known Western Australian seed
oils. J Sci Food Agr 58:585–587
van Heerden FR, Brandt EV, Ferreira D et al (1981) Metabolites from purple heartwoods of the
Mimosoideae. Part 4. Acacia fasciculifera F. Muell ex. Benth: Fasciculiferin, Fasciculiferol, and
the Synthesis of 7-Aryl and 7-Flavanyl-Peltogynoids. J Chem Soc Perkin Trans 1:2483–2490
Van Wyk BE, Gericke N (2007) People’s plants: a guide to useful plants of southern Africa. Briza
Venter F, Venter JA (1996) Making the most of Indigenous trees. Briza Publications, Cape Town/
Pretoria
Wild H, Biegel HM, Mavi S (1972) A Rhodesian dictionary of African and English plant names.
Government Printers, Salisbury
Xu MJ, Wu B, Ding T, Chu JH, Li CY, Zhang J, Wu T, Wu J, Liu SJ, Liu SL, Ju WZ, Li P (2012)
Simultaneous characterization of prenylated flavonoids and isoflavonoids in Psoralea corylifo-
lia L. by liquid chromatography with diode-array detection and quadrupole time-of-flight mass
spectrometry. Rapid Commun Mass Spectrom 26:2343–2358
Yang X, Jiang Y, Yang J, He J, Sun J, Chen F, Zhang M, Yang B (2015) Prenylated flavonoids,
promising nutraceuticals with impressive biological activities. Trends Food Sci Technol
44(1):93–104
Aromatic and Medicinal Plants of Tunisian
Arid and Desert Zone Used in Traditional
Medicine, for Drug Discovery
and Biotechnological Applications
Hanen Najjaa, Abdelkarim Ben Arfa, Ákos Máthé, and Mohamed Neffati
Abstract Tunisian has a high diversity of plants as a result of the variation in

topography climate and edaphic conditions of the country. Among these plants sev-
eral aromatic and medicinal species are traditionally used in folk medicines to treat
and/or manage common ailments. Some others species are used in foods as spices
and/or in aromatherapy. The present chapter endeavors to highlight the botanical
description, geographical distribution, ethno pharmacological uses of 45 species
spread over 22 botanical families among those the most used in traditional medi-
cine. Information on the chemical composition and biological activities of these
species are also included in this chapter.
Keywords MAPs • Tunisian • Arid land • Ethnopharmacology
1 Introduction
The global importance of Medicinal and Aromatic Plants (MAPs) is evident from
the fact that in 2006, the world wide trade of MAPs touched upon 60 billions of US
dollars. With the increase in demand, trade figures are expected to further grow to 5
trillion by the year 2050 (Lang 2008). It is also estimated that Europe alone annually
imports about an average market value of 1 billion US$ from Africa and Asia
(Larsen and Smith 2004; Sher and Hussain 2009). It is also worth mentioning that
the use of wild edible herbs/culinary plants has been increasing in the global food
market for diverse items ranging from salads to desserts. In recent years, there has
H. Najjaa (*) • A.B. Arfa • M. Neffati

Laboratoire d’Ecologie Pastorale, Institut des Régions Arides, 4119 Médenine, Tunisia
Á. Máthé
Faculty of Agriculture and Food Science, University of West Hungary,
Vár 2, H-9200 Mosonmagyaróvár, Hungary

DOI 10.1007/978-94-024-1120-1_8
158 H. Najjaa et al.
been an increasing interest also in the utilization of natural substances. Some ques-
tions concerning the safety of synthetic compounds have also encouraged the study
of plant resources. Essential oils, i.e. volatile secondary substances of plant origin,
can have wide ranging applications in both traditional folk medicine, and food, fla-
voring, cosmetic and fragrance industries. Recently, many essential oils and their
constituents have been investigated for their multifunctional properties (Hajji et al.
2010). Authorities and consumers are equally concerned about food safety and the
potential harmful effects of synthetic additives on health (Reische et al. 1998).
In addition to the increasing demand for safe and natural food in recent years, the
great economic losses caused by the deterioration and poisoning of food products
by food pathogens have motivated many researchers to explore new alternatives to
traditional food preservation practices. Among these natural products, essential oils
(EOs) from aromatic and medicinal plants have received particular attention as
potential natural agents for food preservation (Burt 2004; Bakkali et al. 2008). In
fact, EOs and their main components have been reported to possess a wide spectrum
of antimicrobial, antiviral, insecticidal and antioxidant activities (Janssen et al.
1987; Rota et al. 2004; Gachkar et al. 2007).
The use of plants for medicines is by far the biggest use of plants in terms of the
number of species specifically targeted. Plants provide the predominant ingredients
of medicines in most traditional systems of healing and have been the source of
inspiration for several major pharmaceutical drugs (Chevallier 1996; Sher et al.
2010a). According to Plant life International, the scale of trade in medicinal plants
ranges from local to international. Much of this trade is unrecorded or poorly docu-
mented in official statistics. Due to the poor documentation, decision makers usu-
ally have little awareness of the significance of trade and consumption of medicinal
plants, or of the problems of un-sustainability and sometimes deleterious impacts
on natural habitats of wild collection (Hamilton and Hamilton 2006).
Numerous publications have yielded detailed knowledge of aromatic wild plants
in certain locations in Africa. They have indicated that wild plants are essential
constituents of many African drugs. The ethnobotanical survey and documentation
of traditional knowledge, mainly on the medicinal uses of plants, have provided
important clues for the discovery of new drugs nowadays. For many years, tradi-
tional plant remedies were empirically used in Africa for treating various diseases.
According to the World Health Organization up to 80% of the population in Africa
uses traditional medicine for their primary healthcare (Larsen and Smith 2004;
Al-Quran 2008).
Tunisia has a high diversity of plans with several aromatic plant species tradi-
tionally used in folk medicines, in foods as spices, in massage and in aromatherapy.
Among the 2250 species that compose Tunisia’s vascular flora (Marrif et al. 1995),
1630 species are native to the arid and desert part of the country, which is character-
ized by low rainfall, high temperature and drying winds (Le Houérou 1959).
Remarkably, a wide range of plant species thrive under these conditions, which is of
high economic and ecological significance.
Despite the considerable medicinal plant potential of Tunisian flora, studies on
the traditional uses of many species are relatively scarce. The present chapter aims
Aromatic and Medicinal Plants of Tunisian Arid and Desert Zone Used in Traditional… 159
at reviewing the traditional uses, chemical composition and pharmacological prop-

erties of Tunisian arid plants in order to provide information on their possible uses
as preservatives in food products, in biotechnology or raw materials for pharmaceu-
ticals and cosmetics. Our choice of investigated species is based on two criteria: the
disponibility of ethnopharmacological data indicating their utilization in folk medi-
cine (a) and the recent research results on their chemical composition and biological
activities of their extracts (b).
2 Ethnopharmacological and Culinary Use of Arid Plants
In Tunisia, thanks to the diversity of its climate, the traditional pharmacopoeia con-
sists of a wide arsenal of medicinal plants. Although, many of these species are
nearly facing extinction (Ziyyat et al. 1997), only a small proportion of them has
been scientifically studied.
In this review, the species are listed alphabetically by plant families. For each
species the traditional medicinal and culinary uses are described. The surveyed spe-
cies are listed in Fig. 1, where plant families and species within each family are
cited in alphabetic order. Scientific names and local names are included, and infor-
mation concerning the parts used and the methods of use are given.
Information on the therapeutic properties of plants mentioned in this review
should not be considered as a practical manual for self-medication.
Amaryllidaceae
This family comprises 700 species, widely distributed in the northern hemisphere
(Jones et al. 2004; Tepe et al. 2005). Allium is the largest and the most important repre-
sentative genus of the Amaryllidaceae. For many centuries, several species of
Amaryllidaceae family have been used as vegetables and spices, and as folk medicines
for curing various diseases (Hacıseferoğullari et al. 2005). Many Allium species (Allium
roseum L., Allium cepa L., Allium sativum L.) were well studied in the world as well as
in Tunisia (Najjaa et al. 2007, 2010; Leporatti and Ghedira 2009; Guetat et al. 2010).
Allium species have been found to shown various activities. For example, a wide
array of therapeutic effects of garlic has attracted particular attention in modern
medicines because of its widespread use as antiatherosclerotic, antidiabetic, antihy-
pertensive, antimicrobial, anticancerous, antioxidant, antifungal and antiviral
(Keusgen et al. 2003; Auger et al. 2004; Arnault et al. 2005; Bakri and Douglas
2005; El-Demerdash et al. 2005). According to Durenkamp and Dekok (2004)
Allium species, namely, onion, garlic, leek and chive contain a variety of secondary
sulphur compounds. Sulphur carrying flavour compounds are responsible for the
characteristic smell and taste and are the source of major active compounds which
provide the best known properties in Allium plants (Keusgen et al. 2003; Auger
et al. 2004; Jones et al. 2004; Hacıseferoğullari et al. 2005).
Fig. 1 Aromatic and Medicinal species: [1] Allium roseum, [2] Allium ampeloprasum, [3] Rhus
tripartitum, [4] Periploca laevigata, [5] Capparis spinosa, [6] Hernaria fontanesii, [7] Hamada
scoparia = Arthrophytum scoparium, [8] Artemisia herba-alba, [9] Artemisia campestris, [10]
Oudneya africana, [11] Diplotaxis harra, [12] Colocynthis vulgaris = Cistrullus colocynthis, [13]
Juniperus phoenicea, [14] Ephedra alata, [15] Globularia alypum, [16] Hyparrhenia hirta, [17]
Rosmarinus officinalis, [18] Thymus algeriensis, [19] Thymus vulgaris, [20] Thymus capitatus,
[21] Ajuga iva, [22] Marribium deserti, [23] Marribum alysson, [24] Teucrium polium, [25]
Fig. 1 (continued) Astragalus gombiformis, [26] Calicotome villosa, [27] Genista saharae, [28]
Retama raetam, [29] Ceratonia siliqua, [30] Genista microcephala, [31] Cymbopogon schoenan-
thus, [32] Calligonum comesum, [33] Calligonum azel, [34] Calligonum arich, [35] Polygonum
equisetiforme, [36] Ziziphus lotus, [37] Ruta graveolens, [38] Thymelaea hirsuta, [39] Crithmum
maritimum, [40] Thapsia garganica, [41] Pituranthos chloranthus, [42] Pituranthos tortuosus,
[43] Nitraria retusa, [44] Peganum harmala, [45] Zygophyllum album
Fig. 1 (continued)
Allium roseum L., a polymorphic species, is represented in North Africa by 12 dif-

ferent taxa: 4 varieties, 4 subvarieties and 4 forms (Cuénod 1954; Le Floc’h 1983). In
Tunisia, the same authors mentioned the presence of only three varieties: var. grandi-
florum Briq., var. perrotii Maire. and var. odoratissimum (Desf.) Coss. The last variety
is considered as an endemic taxon in North Africa (Cuénod 1954). A. roseum var.
odoratissimum (Desf.) Coss. prefers poor and sandy soils. It is also found in grassy
and bushy places, cultivated fields and fallows, and roadsides. This taxon is used since
ancient times as a vegetable, spice and herbal remedy (Le Floc’h 1983). Today its
edible aerial parts are widely harvested and sold commercially. The fresh young leaves
and bulbs of A. roseum or “lazoul”, as it is called in Southern Tunisia, are consumed
in salads and used as spice to prepare traditional recipes. Besides its culinary use,
“lazoul” is also used in folk medicine. Le Floc’h (1983) reported its use for the treat-
ment of headaches and rheumatism. It is also used for the treatment of respiratory tract
infections, as an inhalation for colds, to diminish fever and enhance appetite.
Phytochemical screening of A. roseum using TLC showed the presence of anti-
microbially active agents, such as saponins, tannins, flavonoids, coumarins, ste-
roids, cardiac glycosides, free quinone and iridoids (Najjaa et al. 2011a, b). The
dichloromethane, petroleum ether and ethanolic extracts of A. roseum exhibited a
wide spectrum of antimicrobial activities against Staphylococcus aureus,
Staphylococcus epidermidis, Micrococcus luteus, Escherichia coli and Pseudomonas
aeruginosa. Such results may provide a good support for the use of this plant in
herbal medicine. They may also serve as a base for the development of new drugs
and phytomedicines and may be considered as functional food and/or served as a
nutraceutical (Najjaa et al. 2007).
Recent studies revealed that A. roseum var. odoratissimum growing in Tunisia had
high soluble carbohydrate, crude protein and dietary fibre contents, as compared to other
Alliums. Its mineral content was high in potassium, and calcium. The mineral composi-
tion of ‘rosy garlic’ is sufficient in Ca, P, K, Cu, Fe, Zn and Mg so that it can meet many
macronutrient and micronutrient requirements of the human diets (Najjaa et al. 2010)
Allium ampeloprasum L., locally known as “kurrat” in Tunisia, a wild leek species,
originates from the Mediterranean region and southwest Asia (Figliuolo and Mang 2010).
Wild A. ampeloprasum in Tunisia is popularly appreciated as a medicinal plant
or as a vegetable used to prepare local dishes, and it is widespread across the whole
country landscape (Guenaoui et al. 2012). It is rich in flavonoids such as myricetin,
kaemferol and quercetin (Horbowicz and Kotlińska 2000), responsible for anticar-
cinogenic and antithrombotic activities (Havey 1999).
Anacardiaceae
Due to their high contents in phenolics, flavonoids and other phytochemicals, Rhus
species are widely used in both modern and traditional medicine. The extracts
showed antimalarial (Ahmed et al. 2001), antimicrobial (McCutcheon et al. 1992),
antitumorigenic (Lee et al. 2004; Choi et al. 2012), antioxidant (Lee et al. 2002;
Rima et al. 2011; Olchowik et al. 2012), antiviral (Lin et al. 1999), hypoglycaemic
(Giancarlo et al. 2006), leukopenic (Yang and Du 2003), atheriosclerosis (Zargham
and Zargham 2008) and anticonvulsant (Ojewole 2008) properties. In Tunisia, the
genus Rhus is represented by two species: Rhus tripartita (Ucria) Grande [=R. tri-
partitum (Ucria) D.C. = R. oxyacanthoides Dum. Cours. = R. oxyacantha Shousb.
Ex. Cav.] and R. pentaphylla Desf. (Pottier-Alapetite 1979; Le Floch and Boulos
2008). R. pentaphylla Desf. spreads in the north and the center of the country, and is
relieved by R. tripartita from the center to the far southern region. The two species
may be sympatric in several regions. They grow mainly on eroded substrates, under
a rainfall ranging from 100 to 600 mm/year and at altitudes ranging from 10 to
500 m. The populations of both species are widely scattered, presumably as remnant
sites of previously more continuously distributed species. The overharvesting of
roots and other plant parts for traditional medicine, dyeing and tanning (Le Floc’h
1983) has led to a high death rate of trees and a decrease in the size of populations
(Le Houerou 1969). R. pentaphylla Desf. is being lost at a higher rate than R. tripar-
tita. Its habitat has been mainly cleared for agricultural and coal mining operations.
Rhus tripartita (Rh. tripartitum) (Ucria) Grande, is a native presaharan Tunisian
plant, with a great economic and ecological interest. R. tripartitum is classified in
Tunisia among the rare plants that need preservation. This species is adapted to the
sub-deserted climate (Quezel and Santa 1962). R. tripartitum is widespread in North
Africa (Pottier-Alapetite 1981). In Tunisia, R.tripartitum has many traditional uses.
In his ethnobotanical study, Le Floc’h (1983) cited many examples on the consump-
tion of R. tripartitum species. Fruits of this plant are consumed fresh, stored (sweet-
ened), or soaked in sour milk. They are added to drinking water to offer an acceptable
taste (Le Floc’H 1983). The bark is used for tanning and the wood for the collery
and to treat eye troubles. The infusion of the fruits, the leaves and the roots is recom-
mended for gastric ulcer and intestinal ailments. The pulverized leaves or roots or
barks are used on wounds to facilitate healing. Earlier investigations show the
occurrence of vitamin E and flavonoid substances in R. tripartitum extracts
(Mahjoub et al. 2004) and testify the importance of this plant as a source of poly-
phenolics as anti-inflammatory and antioxidant products. They alsodemonstrate the
medicinal and pharmacological interest in R. tripartitum species. Recent work by
chromatographic separation of Tunisian R. tripartitum revealed the presence of four
main natural substances: a new biflavonoid masazinoflavanone (S1), myricetin (S2),
(−)-lyoniresinol 3α-O-β-D-glucopyranoside (S3), and (−)-methyl shikimate (S4)
(Mahjoub et al. 2005). A biflavonoid masazinoflavanone and the isobiflavonoid
calodenone have been isolated and characterized in the methanolic aerial part extract
of the plant R. tripartitum (Mahjoub et al. 2005). Compared with the standard drug
dexamethasone, the biflavonoid substance masazinoflavanone (S1) inhibited, in a
dose related manner, carrageenan-induced paw edema in rats. Mahjoub et al. (2005),
using free radical forms of ABTS and DPPH, tested the antiradical scavenging
capacities of extracts and natural substances from R. tripartitum. The Trolox
Equivalent Antioxidant Capacity (TEAC) assay shows values of 1.92, 1.39, 1.25,
2.40 and 1.58 mM Trolox/mg sample respectively for the chloroformic extract, the
ethyl acetate extract, (S1), (S2) and (S3). Values revealed from the total phenolic
content assay were 299.3, 372.3, 99.1 and 235.7 mg Catechin/g extract respectively
for the chloroformic, ethyl acetate, ethyl acetate/methanol and methanolic extracts
(Mahjoub et al. 2010).
Asclepiadaceae
Periploca laevigata Ait. is native to the Mediterranean region and widely distrib-
uted in the Sahara area. P. laevigata is an important pastoral species in the dry season.
This shrub grows mainly in the semiarid and sub-humid zones, between 400 and
1800 m. Having disappeared from large sites of its former distribution area but still
being quite frequent on some islands, it is classified as endangered (EN). In Tunisia,
it is predominantly found in the south of the country, especially in the mountains. It
is used as an herbal preparation (tea) because of its reputed medicinal properties, e.g.
for the treatment of headaches and diabetes (Askri et al. 1989). Its utilization for
animal skin tanning mentioned by Chevalier (1935), has been confirmed by inhabit-
ants of Fogo, but nowadays, it is rarely practiced. On Santiago it is used as a medici-
nal plant to treat fever and cough (Gomes et al. 1999). The root powder of P. laevigata
is used for preparing soft drinks and as an aromatic, in Tunisia. The plant is used also
to treat digestive disorders and hypertensive effects, as well as other health problems
(Hajji et al. 2009). The most studied Periploca species, were reported to have various
biological activities, such as anti-proliferative (Spera et al. 2007), antitumor (Itokawa
et al. 1988) and hypotensive effects (Askri et al. 1989).
Many chemical compounds have been isolated and identified from species of the
Periploca genus, such as α- and β-amyrin, lupeol β-sitosterol and elemane-type
sesquiterpenes from P. laevigata (Askri et al. 1989). Gas chromatography/mass
spectrometry was used to determine the composition of the P. laevigata root bark
oil. Forty-three components were identified in the essential oil and the main com-
pounds were benzaldehyde (56%), methyl 4-methoxysalicylate (6.55%) and carva-
crol (4.75%). The P. laevigata root bark essential oil exhibited a dose-dependent
manner of inhibitory activity toward ACE. The highest ACE inhibitory activity
(54%) was observed at a concentration of 30 μg/mL. The P. laevigata root bark oil
was also found to possess antioxidant activities, as evaluated by the 1,1-diphenyl-2-
picrylhydrazyl (DPPH) radical method, β-carotene bleaching and reducing power
assays. The antimicrobial activity of the essential oil was also investigated on
several microorganisms. The inhibition zones and minimal inhibitory concentration
(MIC) values of bacterial strains were in the range of 12–46 mm and 50–300 μg/ml,
respectively. The inhibitory activity of the P. laevigata root bark essential oil against
Gram-positive bacteria was significantly higher than against Gram-negative. It also
exhibited remarkable activity against several fungal strains (Hajji et al. 2010). Our
findings demonstrate that P. laevigata essential oil might be a good candidate for
further investigations of new bioactive substances (Hajji et al. 2010).
Capparidaceae
Capparis spinosa L. is a perennial shrub and is the common name of the genus
Capparis, family Capparidaceae. This genus is represented by several species
(about 250) of which the most important economical species is C. spinosa. It is
known by various names, e.g. Caper (English), Kabbar (Arab), Alcaparro (Spain),
and Gollaro (Pakistan). Capers grow wildly in various regions of the world, espe-
cially in the Mediterranean basin, showing strong resistance to hard environmental
conditions. Caper is adapted to poor soils, and is widespread on rocky areas, moun-
tains and grows on numerous soil types, including alfisols, regosols and lithosols. It
shows a good response to volcanic or alkaline soils. Soil pH from 6.1 to 8.5 is toler-
ated (Janick and Paull 2006). This perennial shrub with a height of 30–100 cm, has
thick and deep roots, and can be thorny or inerm (Inocenio et al. 2006 and Saadaoui
et al. 2007).
As a spontaneous plant, caper has a wide natural distribution. Despite adverse
conditions, plants of Capparis, do not seem to show any water stresses or any symp-
toms of photo-inhibition. The plants efficiently utilize the high irradiance through-
out the growth season (Levizon et al. 2004). In Tunisia the average annual caper
production is estimated to be around 150 tonnes. The USA is one of the most impor-
tant consumers (Janick and Paull 2006). The cultivation of this plant is limited.
Most of the supplies for traditional and commercial uses of Capparis depend exclu-
sively on the collection of wild plants. Capers are cultivated also in some other
countries, e.g.: Spain, Italy, Turkey, Morocco, Tunisia, Algeria, Iran and the coastal
areas of the Black Sea. Caper cultivation started around 1970 in Spain and Italy, and
later in Morocco, with a maximum of about 4000, 1000, and 2500 ha in cultivation,
respectively (Infantino et al. 2007). It is worth mentioning that various studies con-
ducted on the pharmacological potential of C. spinosa have verified its local, folk
medicinal uses.
The aromatic part of the caper plant is the floral bud, which is gathered just before
it blossoms. Before commercial packaging, the buds are pickled in vinegar or pre-
served in granular salt (Germano et al. 2002). They have long been used in recipes of
salads, pasta, meat, sauces and garnishes to add a pungent spicy flavor and aroma to
food. Fici (2001) stated that the salted and pickled caper bud is often used as a sea-
soning and garnish. Furthermore, he also reported that Capers are common ingredi-
ents in the Mediterranean cuisine, especially Italian and it is also important in the
commercial preparation of frozen food (Germano et al. 2002). Capers are graded and
sold by their size, defined as follows, with the smallest sizes being the most valuable:
Non-pareil (up to 7 mm), surfines (7–8 mm), capucines (8–9 mm), capotes (9–11
mm), fines (11–13 mm) and grusas (14+ mm). Capers are picked daily, since the
youngest flower buds (about the size of peas) have the highest quality. The young
fruits and tender branch tips can also be pickled and used as a condiment. More
rarely, mature and semi-mature fruits are eaten as a cooked vegetable. The ripened
fruits are rich from protein, lipid, carbohydrates, and vitamins and mineral (Fragiska
2005; Ozcan 2005) reported that outside of the Mediterranean and the Caucasus
mountains, capers are not much known, although the pickled fruits of some Central
Asian species (e.g., Capparis aphylla) are used as a vegetable in Afghanistan,
Pakistan and North Western India (Sher and Alyemeni 2010).
Roots, leaves, buds, fruit, bark and seeds of caper were used by ancient peoples
for medicinal purposes, to treat some diseases such as rheumatism, stomach prob-
lems, headache and toothache. The first use of C. spinosa was for medicinal pur-
poses, in 2000 BC by the Sumerians and afterwards by Greeks and Romans (Romeo
et al. 2007). Different parts of the caper plant can be used as a drug or a cosmetic. In
ancient times, roots were consumed by the ancient Egyptians and Arabs to treat kid-
ney disease, liver disease, stomach problems and scorpion’s stings. Leaves were used
by the ancient Arabs against skin diseases, to treat earaches and to kill worms in the
ear. Buds were used by the ancient Arabs against spleen disease. Ancient Romans
used this part of the caper plant to treat paralysis. Flowers could serve as a stimulant
to increase erection and soothe pains. Fruits were used by ancient Greek to treat
convulsions. Seeds served as a medication for gum problems by ancient Arab and to
accelerate menstruation by ancient Egypt and ancient Greek (Rivera et al. 2003).
Actually, in Morocco the fruit of C. spinosa is used to cure diabetic (Eddouks
et al. 2002) Iranian people use the roots, fruit and plant bark of C. spinosa as diuret-
ics, tonics, against malaria and joint disease (Hooper 1937; Afsharypuor et al.
1998). In Pakistan, people use all parts of C. decidua as anti-rheumatic, as diuretic,
as kidney disinfectant, tonics, to reduce flatulence, to improve liver functions, to
cure arteriosclerosis and migraine (Jagtap et al. 2006; Pirzada et al. 2007); and they
especially used leaves of C. spinosa as analgesic, antihemorrhoid, antirheumatic
(Khakberdyev et al. 1968), aperients, deobstruent, depurative and diuretic (Chopra
et al. 1986). In India, buds and roots of C. spinosa are useful in the treatment of
boils; leaves are used as counter-irritant and as a cataplasm in swellings; roots are
used to treat fever, rheumatism, paralysis, toothache and kill worm in the ear; the
bark is used in the treatment of coughs, asthma and inflammation (Wealth of India
1992; Kiritikar and Basu 1987, 1991). These findings are in line with the study of
Fragiska (2005), who reported that C. spinosa has the potential to support the for-
mations of different agro-based industrial products. Moreover, Sushila et al. (2010)
also reported that in ayurvedic medicine capers are recorded as hepatic stimulants
and protectors, improving liver function and also act as a appetizer agent.
The presence of both α- and γ-tocopherol was found in buds. Moreover, C. spi-
nosa contained an appreciable level of vitamin-C. Several studies have linked plants
rich in these compounds with a reduced risk of several chronic and degenerative
diseases including cancer, cardiovascular disorders and atherosclerosis (Brigelius-
Flohé and Traber 1999; Sommer and Davidson 2002).
Tlili et al. (2010), show that leaves and flower buds of C. spinosa from different
locations in Tunisia were very rich in total phenolic compounds (with an average of
3643.29 and 2621.14 mg/100 g FW, respectively), including rutin (with an average
of 1352.71 and 693.14 mg/100 g FW, respectively). The highest value of phenolic
compound and tocopherol was detected in samples from Tataouine, arid zone (3.96
± 0.40 mg/100 g FW) while the lowest value was detected in samples from Ghar el
Melh (2.19 ± 0.12 mg/100 g FW). This difference may be due to the effect of the
location, and confirms our hypothesis that plants collected from stressed zones syn-
thesize more bioactive substances. HPLC was used to determine carotenoids
(β-carotene, lutein, neoxanthin, and violaxanthin) and tocopherols in leaves, buds,
and flowers of Tunisian C. spinosa (Tlili et al. 2010). The means of total carotenoids
were 3452.5; 518.5, and 342.7 μg/g fresh weights (FW) in leaves, buds, and flowers,
respectively. The principal form of tocopherol detected in leaves was α-tocopherol
(20.19 mg/100 g FW). In buds and flowers, they are also present at 49.12 and 28.68
mg/100 g FW, respectively, whileγ-tocopherol content amounted to 48.13 and 27.8
mg/100 g FW, respectively. The combined content of pro-vitamin-A and vitamin-E
in capers encourages researchers to carry on with research and to explore this plant
(Tlili et al. 2009). The glucosinolatecontent and composition of young shoots and
raw flower buds of C. spinosawere investigated by HPLC with UV detection.
Samples were harvested in August 2001 in Turkey. Twelve different glucosinolates
were identified in the young shoots and buds of both species. Total content of glu-
cosinolates ranged from 6.55 μmol/g of C. spinosa to 45.56 μmol/g (young shoots
of C. ovata). The main glucosinolate was glucocapperin, which amounted to 90% of
the total glucosinolates (Germano et al. 2002). Some publications refer to specific
aspects of the qualitative composition of flavonoids, the occurrence of elemental
sulfur, and physical and chemical properties (Rodrigo et al. 1992; Ozcan and Akgul
1998; Brevard et al. 1992; Inocencio et al. 2000), but up to now there is little avail-
able information about the glucosinolate content and composition of members of
this family. The presence of different glucosinolates such as neoglucobrassicin, 4-
methoxyglucobrassicinglucocapperin, glucoiberin, sinigrin, 1-methoxy-3-
indolylymethyl, and glucobrassicin were described (Daxenbichler et al. 1991; Kjaer
and Thomsen 1963; Schraudolf 1989), mostly in the roots or leaves.
Besides bitter flavonoid glycosides, rutin, quercetin, quercetin 3-O-glucoside
and quercetin 3-O-glucoside-7-O-rhamnoside, quercetin 3-O-(6′″-α-L-rhamnosyl-
6″-β-D-glucosyl)-β-D-glucoside (Sushila et al. 2010), and kaempferol glycosides,
C. spinosa also contains lipids, glucocapparin (methyl glucosinolate), methyl isothio-
cynate, isopropyl isothiocyanate, sec-butyl isothiocyanate, benzyl-isothiocyanate,
β-sitosterylglucoside-6′-octadecanoate, 3-methyl-2-butanyl-β-glucoside, stachy-
drine (a pyridine alkaloid), and cadabicine (a 24-membered polyamine lactam alka-
loid). Furthermore, homologous polypreols namely; cappaprenol-12,
cappaprenol-13, and cappaprenol-14 with 12, 13, and 14 isoprene units were also
isolated from the alcoholic extract of C. spinosa (Al-Said et al. 1988; Inocencio
et al. 2002). Later, new (6S)-hydroxy-3-oxo-α-ionol glucosides together with cor-
choinoside C (6S, 9S)-roseoside, and prenyl glucosides were isolated from the
mature C. spinosa fruits (Calis et al. 2002). C. spinosa fruits also contained cappa-
riloside-A, stachydrine, an adenosine nucleoside, hypoxanthine, β-sitosterol, vanil-
lic acid, p-hydroxybenzoic acid, protocatechuric acid, daucosterrol, uracil,
butanedioc acid, and uridine (Calis et al. 2002). In the volatile oils of C. Spinosa 145
compounds were identified. The major constituents were: aldehydes 22%, esters
21%, and sulfur containing compounds 8.42%. The presence of sesquiterpenes,
monoterpenes, and capric acid was also confirmed (Arena et al. 2008). Chemical
studies on caper have reported the richness of different partsin several beneficial
chemical compounds. The significant level of all theses antioxidants highlight the
important nutritional and medicinal value of this species used in the Mediterranean
basin (Tlili et al. 2010).
Recent works reported that the C. spinosa extract has the ability to maintain skin
homeostasis and can be used as a possible tanning agent or as a treatment for hair
de-pigmentation (Matsuyama et al. 2009). Methanolic extract of C. spinosabuds
may contribute to improve the immune surveillance of human peripheral blood
mononuclear cells toward virus infection by up-regulating expression of peculiar
pro-inflammatory cytokines; it suppressed the replication of herpes simplex virus
type 2 and increased the expression of pro-inflammatory cytokines including inter-
leukin-12, interferon-γ and tumor necrosis factor-α (Arena et al. 2008). More
recently, a protein with potent antiproliferative activity toward tumor cells and
inhibitory activity toward HIV-1 reverse transcriptase and some antifungal activity
have been isolated from seeds of C. spinosa (Lam and Ng 2009). These important
biological activities were probably due to the important quantity of the antioxidants
which have an excellent activity against these diseases. It was reported that aqueous
extracts of C. spinosaexhibit a potent anti-hyperglycemic activity in diabetic rats
without affecting basal plasma insulin concentrations (Eddouks et al. 2004, 2005).
These facts explain the use of caper by old people to treat diabetic disease (Jouad
et al. 2001). Yue-lan et al. (2010) call attention to the notable protective activity of
C. spinosa against oxidative stress and interrupting of ROS in systemic sclerosis
dermal fibroblasts. Ethanolic extracts from C. spinosacan effectively inhibit fibro-
blast proliferation and type I collagen production in progressive systemic sclerosis
(Cao et al. 2008). Aqueous extracts of C. spinosacan also act by decreasing the
cholesterol biosynthesis (Eddouks et al. 2005). Gadgoli and Mishra (1999) sug-
gested that C. spinosaextract possesses significant antihepatotoxic activity against
carbontetrachloride and paracetamol which induced hepatotoxicity in vivo and thio-
acetamide and galactosamine which induced hepatotoxicity in isolated rat hepato-
cytes, using in vitro technique. This result supports the use of caper by the ancient
people to treat liver disease and improve liver functions (Jagtap et al. 2006).
The anti-oxidant activity of cappariside, 4-hydroxy-5-methylfuran-3-carboxylic
acid and several other organic acids from C. spinosa was confirmed (Tlili et al.
2009, 2010; Sher et al. 2010a). New anti-inflammatory and antioxidant compounds,
lignin glucosides, 1H-indole-alkaloid glucosides, and phenolic glucosides were iso-
lated from roots of another caper species C. tenera. Nonetheless, cappaprenol-13
isolated from C. spinosa showed significant anti-inflammatory activity and antioxi-
dative potential (Al-Said et al. 1988). C. spinosa extract was shown to possess
prominent activity against Plasmodium falciparum (Arena et al. 2008). The chono-
droprotective effect of C. spinosa suggested that it might be used in the manage-
ment of cartilage damage during the inflammatory process (Tlili et al. 2009). In
diabetic rats, C. spinosa treatment, significantly reduced plasma glucose, triglycer-
ide levels and plasma cholesterol levels (Sher et al. 2010a). On the other side, the
extracts of C. spinosa flowering buds, showed the ability to protect against
histamine-induced bronchospasm (Arena et al. 2008).
A popular compound finished herbal drug formulation Liv-52 (containing C.

spinosa as its essential ingredient) was used in patients with liver cirrhosis follow-
ing a hospital based clinical protocol. Liv-52 showed significant hepato-protective
effect in cirrhotic patients. The protective effect of Liv-52 was attributed to the
diuretic, anti-inflammatory, anti-oxidative, and immunomodulating properties of
the component herbs (Arena et al. 2008). However, antihepatotoxic activity of
p-methoxy benzoic acid isolated from C. spinosa was confirmed by earlier research-
ers (Gadgoli and Mishra 1999). It is worth mentioning that the ethanol extract of C.
spinosa fruits exhibited a notable protective activity against oxidative stress and
interrupting of ROS-ERK1/2- Ha-Ras signal loop in systemic sclerosis, signifying
its potential protective effects against skin sclerosis (Sher et al. 2010a). The same
researchers also reported that the ethanol extract from C. spinosa was found to
effectively inhibit the fibroblast proliferation and type I collagen production in pro-
gressive systemic sclerosis (Zhang and Tan 2009). Herpes simplex virus type 1
(HSV-1) and type 2 (HSV-2) are common human pathogens that in particular cases,
can also cause severe problems especially in immunodeficient patients. The metha-
nol extract of C. spinosa contributed well to improving immune surveillance of
PBMCs (human peripheral blood mononuclear cells) toward virus infection by up-
regulating expression of peculiar pro-inflammatory cytokines; indicating the possi-
bility of successful use of C. spinosa for treatment of HSV-2 infections in
immunocompromised hosts (Arena et al. 2008). In a recent study, it was shown that
the male flowers of C. spinosa save resources for the female function and that they
primarily serve to attract pollinators as pollen donors (Zhang and Tan 2009). A
monomeric protein with a molecular mass of 38 kDa was also purified from C. spi-
nosa seeds. The protein exhibited an N-terminal amino acid sequence with some
similarity to imidazo-leglycerol phosphate synthase. It inhibited HIV-1 reverse tran-
scriptase and mycelia growth in fungus without having hemogglutinating, ribonu-
clease, mitogenic or protease inhibitor properties. Furthermore, a novel dimeric
62-kDa lectin was purified from caper (C. spinosa) seeds which inhibited HIV-1
reverse trans-criptase and proliferation of both hepatoma HepG2 and breast cancer
MCF-7 cells (Lam et al. 2009). Based on the current detailed ethnopharmaceutical
surveys and literature reviews, it is concluded that C. spinosa. Besides medicinal
significance, various parts of C. spinosa were found to have food value, potential
culinary value, and possessed also cosmetic ingredients. It is worthy to conclude
that C. spinosaas a key to resources for raw materials in the pharmaceutical, aroma
and food industries, hence it is called “Plant of the Millennium” (Sher and Alyemeni
2010). Based on the present literature review C. spinosa is a safe plant and there are
no reports in the scientific literature on its toxic manifestations after acute, sub-
acute, or chronic treatment. However, allergic contact dermatitis caused by C. spi-
nosa was reported (Angelini et al. 1991).
Caryophyllaceae
Herniaria fontanesii J. Gay, which have a widespread distribution in the

Mediterranean area (Borkowski and Pasich 1958), have been until now little stud-
ied. In Tunisia, this plant has been used traditionally for Lithia’s problems, as
diuretic and for healing wounds and scars. In the traditional pharmacopoeia of
Morocco, the aerial parts of this plant are used for the treatment of lithiasis (Mbark
et al. 1999).
In a previous investigation into the secondary metabolites of H. fontanesii, a tri-
terpenoid saponin (herniaria saponin A), flavonol derivative, isorhamnetin
3-(3′″-feruloylrhamnosyl(1-->6)galactoside), isorhamnetin 3-robinobioside and
(−) catechin was also isolated from the aerial parts of H. fontanesii (Mbark et al.
1995, Mbark et al. 1996). The structures of the two saponins isolated from the aerial
parts of this specieswere established as 28-O-{a-L-rhamnopyranosyl-(1-->2)-((α-L
rhamnopyranosyl)-(1--->3))-(β-D-4-acetoxyfucopyranosyl}ester of 3-O-(α
-L-rhamnopyranosyl-(1 --> 2)- β -D-glucopyranosyl uronic)-2 β-3 β-16-α
-trihydroxyolean-12-ene-23, 28-dioic acid and 28-O-{α-L-rhamnopyranosyl-(1-->
2)-((α -L-rhamnopyranosyl (1--> 3))-(β -Dfucopyranosyl} ester of 3-O-(α
-L-rhamnopyranosyl-(l--->2)- β -D-glucopyranosyl uronic)-2 β-3 β-16- α
trihydroxyolean-12-ene-23, 28-dioic acid (Mbark et al. 1995) and oleanane sapo-
nins (Mbark et al. 1999).
Chenopodiaceae
Hammada scoparia (Pomel) Iljin (=Arthrophytum scoparia (Pomel) Iljin =

Haloxylon articulatum subsp. scoparium (Pomel) Batt.= Haloxylon scoparium
(Pomel) is a small, highly-branched halophytic shrub distributed in south-east
Spain, North Africa and parts of Iran, Turkey, Iraq and Syria (Irano-Turanian region)
(Maire 1962). A. scoparium grows in North Africanregions, especially in Tunisia,
Algeria and Morocco, with an extreme environment ofsalinity, dryness and sun-
light. Given this type of environment, A. scoparium iswell-adapted to water short-
ages and exhibits adaptations for storing water; in addition, surviving harsh
environmental conditions might cause it to produce bioactiveanti-stress factors.
Conventionally, A. scoparium is usually used as traditional medicine to treat eye
disorders, a poultice for mould, and as a snuff powder. The ash of H. scoparia or
“Remeth” as called in Tunisia is blended with the powder of tobacco leaves for the
preparation of the tobacco to appraise “Neffa”. This tobacco has numerous thera-
peutic virtues of which the most important remains her action in the treatment of the
scabies of the sheep. As an infusion, the plant is also used to struggle against the
diarrhea and for treatment in case of bites of snakes and against rheumatisms. By
blending dry leaves with Pistacia atlantica and Cleome arabica, this plant is used
against headaches.
Recently, Aquoues extract of A. scoparium has been reported to show antioxida-

tive activity, hepatoprotective effects (Bourogaa et al. 2012), antibacterial activity
(Alghazeer et al. 2012) anti-cancer, anti-plasmodial and larvicidal activity (Bellei
et al. 2010; Bourogaa et al. 2011). Bourogaa et al. (2012) tested the antioxidative
activity and hepatoprotective effects of methanolic extract (ME) of H. scoparia
leaves against ethanol-induced liver injury in male rats treated daily with 35% etha-
nol solution (4 g kg−1 day−1) during 4 weeks. This treatment led to an increase in the
lipid peroxidation, a decrease in antioxidative enzymes (catalase, superoxide dis-
mutase, and glutathione peroxidase) in liver, and a considerable increase in the
serum levels of aspartate and alanine aminotransferase and alkaline phospahatase.
However, this treatment protects efficiently the hepatic function of alcoholic rats by
the considerable decrease in aminotransferase contents in serum of ethanol-treated
rats. The glycogen synthase kinase-3 β was inhibited after ME administration,
which leads to an enhancement of glutathione peroxidase activity in the liver and a
decrease in lipid peroxidation rate by 76%.
H. scoparia has been described as a plant rich in alkaloids (Carling and Sandberg
1970; Benkrief et al. 1990; Jarraya et al. 1993, 2008; Jarraya and Damak 2001). The
molluscicidal activity of H. scoparia leaf extracts and the principal alkaloids iso-
lated from them (carnegine and N-methylisosalsoline) were tested against the mol-
lusc gastropod, Galba truncatula, the intermediate host of Fasciola hepatica in
Tunisia. The results indicated that the molluscicidal activity was correlated with the
presence of alkaloids. A significant molluscicidal value, according to the World
Health Organization, was found with the methanol extract (LC50 = 28.93 ppm).
The effect of A. scoparium ethanol extract (ASEE) on melanogenesis regulation
in B16 murine melanoma cells was investigated by Chao et al. (2013). Cells treated
with 0.017% (w/v) ASEE showed a significant inhibition of melanin biosynthesis in
a time-dependent manner without cytotoxicity. To clarify the mechanism behind the
ASEE-treated melanogenesis regulation, the expressions of tyrosinase enzyme and
melanogenesis-related genes were determined. Results showed that the expression
of tyrosinase enzyme was significantly decreased and Tyr, Trp-1, Mitf and Mc1R
mRNA expressions were significantly down-regulated. LC-ESI-TOF-MS analysis
of the extract identified the presence of six phenolic compounds: coumaric acid,
cinnamic acid, chrysoeriol, cyanidin, catechol and caffeoylquinic acid. The melano-
genesis inhibitory effect of ASEE may therefore be attributed to its catechol and
tetrahydroisoquinoline derivative content. Chao et al. (2013) report that the first
report on the antimelanogenesis effect of A. scoparium and on its potential as a
whitening agent. Flavonol triglycoside, isorhamnetin: 3-O-beta-D-xylopyranosyl-
(1–3)-alpha-L rhamnopyranosyl-(1″′-6″)-beta-D-galactopyranoside, Isorhamnetin
3-O-beta-D-apiofuranosyl-(1–2) (alpha-L-rhamnopyranosyl-(1–6))-beta-D-
galactopyranoside, Isorhamnetin3-O-alpha-L-rhamnopyranosyl-(1′-2″) (alpha-L-
rhamnopyranosyl-(1′-6″))-beta-D-galactopyranoside have been isolated from
leaves of H. scoparia collected from Tunisia (Ben Salah et al. 2002).
Compositeae/Asteraceae
Artemisia herba-alba Asso. is a greenish-silverperennial dwarf shrub growing in

arid and semi-aridclimates. This species is known as “desert wormwood” in English,
‘armoise blanche’ in French, and ‘chih’ in Arab, and it is characteristic of the steppes
and deserts of the Middle East, North Africa (Tunisia, Algeria and Morocco) and
Southern Europe (Spain and Italy), extending into the Northwestern Himalaya
(Vernin et al. 1995). In Tunisia, A. herba-alba is found from the mountains around
Jebel Oust (Fahs) until the South of the country (Nabli 1989; Marrif et al. 1995). A.
herba-alba is used as aromatisant for tea and in folk medicine for treatment of
colds, coughing, and intestinal disturbances and as antidiabetic agent (Le Floc’h
1983).
This plant is widely used in the traditional medicine to treat diabetes, bronchitis,
diarrhea, hypertension and neuralgias (Said et al. 2002; Tahraoui et al. 2007). The
essential oil of this species was known for its therapeutic disinfectant, anthelmin-
thic, antibacterial and antispasmodic virtues (Hatimi et al. 2001). Moreover, the oil
exhibited antileishmanial (Hatimi et al. 2001), spasmolytic (Perfumi et al. 1999),
antioxidant (Mighri et al. 2010b) and antimutagenic activity against the carcinogen
benzopyren (Neffati et al. 2008). Numerous studies in the literature were focused on
its essential oils and have reported the composition of A. herba-alba essential oil
from different parts of the world and confirmed the many oil-dependent chemotypes
assigned to the plant. According to the reviews of Lawrence (2003) and Salido et al.
(2004), two types of oils could be distinguished: those whose composition is domi-
nated by a major compound (camphor, β- or α-thujone, chrysanthenone, chrysan-
thenyl acetate, or davanone) and those characterized by the codominance of two or
more of these compounds. According to Al-Mustafa and Al-Thunibat (2008), vari-
ous compositions were observed for oils isolated from the aerial parts of A. herba-
alba plants growing wild in Tunisia. In Tunisia (semi-arid and arid land), various
compositions were observed, dominated either by a single component (α-thujone,
β-thujone, 1,8-cineole, camphor, chrysanthenone or trans-sabinyl acetate) or char-
acterized by the occurrence, at appreciable content, of two or more of these com-
pounds (Akrout 2004; Boukrich et al. 2010; Mighri et al. 2010a). In Northern
Tunisia, the major component was either α-thujone (48.7–79.9%), chrysanthenone
(44.2–64.8%), or camphor (35.5 – 47.9%) for 13 oil samples out of 18, and in the
five other samples, the main component did not exceed 34%. In Southern Tunisia,
four oils out of eight contained a major component present at very high content, i.e.,
a-thujone (50.6–79.9%) or trans-sabinyl acetate (43.8%). The four other composi-
tions were characterized by the occurrence, at appreciable contents, of two or more
compounds (Al-Mustafa and Al-Thunibat 2008). Oxygenated monoterpenes were
found to be the major components of A. herba-alba oil extracted from aerial parts
of plants originated from arid regions of Tunisia (Akrout 2004; Neffati et al. 2008).
Studies from Spain (Salido et al. 2001, 2004) showed that monoterpene hydro-
carbons and oxygenated monoterpenes are the most abundant skeletons in A. herba-
alba oil, but large amounts of sesquiterpenes were found for some populations. Two
oil types were found for plants grown in Sinai (Feuerstein et al. 1986) those of
cineole-thujane-borane type and the pinane type with monoterpene skeletons. In
Jordan regular monoterpenes were predominant and the principal components were
α- and β-thujones, classifyng the plant as being a thujone chemotype (Hudaib and
Aburjai 2006). In Morocco, the market leader in A.herba-alba essential oil exports,
16 chemotypes were found (Lamiri et al. 1997a, b), with 12 having monoterpenes
as major components and for four, sesquiterpene skeletons represent the major frac-
tion of the oil.
Artemisia campestris L. “Field sagewort” is a perennial scarcely aromatic herb or
small shrub. A. campestris “T’gouft” is medicinal plants commonly used by local
population in the southern of Tunisia for several purposes.
The aerial part of this plant is used in popular medicine as anthelmintic, antisep-
tic, cholagogue, deobstruent, emmenagogue, stomachic, tonic, hypotensive and
antivenin. This plant was used by some native North American Indian tribes as an
abortifacient to terminate difficult pregnancies. This plant has been crushed and
applied externally to rheumatic joints, eczema, bruises and sores. A poultice of the
crushed leaves has been applied to sore eyes. An infusion of the roots has been used,
especially on children, as a hair tonic and to treat scalp infections. It has been taken
internally to promote urination and bowel movements (Le Floc’h 1983). The essen-
tial oils of A. campestris widespread in the south of Tunisia have been studied by
several authors and were found to contain different compounds such as alpha and
beta-pinenes, p-cymène, caryophyllene oxide, spathulenol, limonene, dehydro-1,8-
cineole, cadin-4-en-7-ol, gamma-terpinene, (Z)-beta-ocimene, aromadendrene, ger-
macrene D, bicyclogermacrene, myrtenol, p-cymen-8-ol, gamma-cadinene,
ar-curcumene, delta-cadinene, calamenene, alpha-muurolene, gamma-muurolene,
gamma-cadinene, bisabolene and endoperoxide, (Z,E)-farnesol, cedrol and verben-
one (Neffati et al. 2008; Akrout et al. 2001, 2003). Solvent extracts and essential oils
from Artemisia campestris have been shown to exhibit antioxidant, hepatoprotec-
tive, antibacterial, antiviral, insecticide and allelochemical activities (Hatimi et al.
2000; Djeridane et al. 2006; Aniya et al. 2000; Tharib et al. 1983).
Crucifereae
Oudneya africana R. Br., a wild-growing plant in the arid region of Tunisia, is
used in ethnomedicinal treatment of microbial infections. Later, Hammami et al.
(2009) shown that compounds isolated from O. africana have broad-spectrum activ-
ity against both Gram-positive and Gram-negative bacteria.
Djeridane et al. (2006) considered this species as medicnal plant in Algeria to
treat the digestive problem and they reported that the aqueous ethanol of O. africana
characterised by 7.75 mg GAE/g dw ± 0.22 total phenolic content and 7.66 mg rutin
equivalent/g dw ± 0.23 flavonoids content and has an antioxidant activity expressed
as Trolox equivalent antioxidant capacity (TEAC) (16.30 (mmol TEAC/g dw).
Diplotaxis harra (Forsk.) Boiss. an annual is widely distributed in many sandy

and gypseous areas in southern Tunisia (Tlig et al. 2008). It is a ramified stem herb
which occurs in both sandy and gypseous soils reaching a height of 50–60 cm
(Chaieb and Boukhris 1998; Pottier-Alapetite 1979), and grows best in soils where
NaCl levels are around 580 ppm.
When dried, this herb is browsed and appreciated by animals but its abuse seems
to have certain toxicity; however, the fresh plants are generally refused by the ani-
mals (Le Floc’h 1983). It is also a medicinal plant used against the scabies of the
animals. The decoction of the D. harra leaves was used against constipation (Boulos
1983) and showed higher activity against yeasts than bacteria (Hashem and Saleh
1999).
Cucurbitaceae
Colocynthis vulgaris (L.) Schard. = Cistrullus colocynthis Schrad. a plant indig-

enous to India, Pakistan and Sri Lanka have a variety of ethnomedical claims and
finds uses as a purgative (Banarjee and Dandiya 1967), powerful cathartic (Adam
1978), abortifacient (Saha et al. 1961) and for amenorrhea (Al-Rawi and Chakravarty
1964). Extracts derived from fruits of C. vulgaris have been shown to possess car-
diac depressant, smooth muscle relaxant, cytotoxic, and antitumour activities (Faust
et al. 1958). Previous chemical investigations of the fruits of C. vulgaris by chroma-
tography technic has revealed the presence of cucurbitacins B, D, E, I, L and the
glucosides of cucurbitacins B, E and L (Darwish-Sayed et al. 1974).
C. vulgaris is used for rhemnatism, the Bedouins tie a slice of fresh, green gourd
onto the heel before retiring. The gourds are eaten by domestic animals when green,
according to an Abadi Bedouin of the Eastern Desert. Mason (1936) learned, during
his travels in the Libyan Desert that they were eaten (when green) by gazelles,
Barbary sheep and donkeys, probably, he postulated, for the water they contained.
He tasted a juicy piece of green gourd and hod diarrhoea for 3 days. Tregenza (1955)
guides told him that gazelles broke the dry gourds with their horns to get at the
seeds. According to this author, the Tibbu people harvested the seeds of handal for
food. The seeds were eaten after prolonged soaking in water. According to Hassanein
Bey (1925) abra, the staple dish of the Tibbu people, is made from handal. The
seeds are boiled thoroughly to get rid of the bitterness and then crushed with dates
or locusts in a wooden mortar. Hassanein Bey recorded a conversation with a
Bedouin who had once survived for 12 days on handal seeds which, the man said,
had upset his digestion. The cathartic effect of the untreated seeds is known to all
Bedouins.
In Bahariya Oasis learned that a purgative for intestinal parasites is milk that has
stood overnight in a hollowed, dry ground gourd. Since the time of the Pharaohs
Natives of Aswan dried gourds with a small opening in them have been placed
among clothes to keep away insects.
Cupressaceae
Juniperus phoenicea L.
The genus Juniperus consists of approximately 60 species growing in the Northern
Hemisphere and divided into three sections: Caryocedrus Juniperus (=Oxycedrus)
and Sabina, the third being the most important one which comprises J. phoenicea.
Recently, Adams (1999) showed on the basis of essential oil composition and RAPD
DNA fingerprinting that J. phoenicea is distinguishable from other species of sec-
tion Sabina. J. phoenicea is a shrub or a small tree from the Mediterranean region
(Bonnier and Douin 1990). In Mediterranean countries the Juniperus flora is
expanding to a great variety of natural habitats (Milos and Radonic 2000; Adams
2000; Munoz-Reinoso 2004) and has attracted special interest as constructional tim-
ber, because its wood is free of insults from pests and diseases. Another interesting
use of Juniper wood was described by Herodotus, who connected the beneficial
effects of plant resin with the preservation of dead human tissues in ancient Egypt.
This activity was further confirmed by Dioscorides, who named Juniper as the “life
of the dead”. Later, the insect-repellent properties of Juniper were widely recog-
nized through biological evaluation experiments that revealed its strong antibacte-
rial and fungicide properties (Karaman et al. 2003). In addition, the EO of Juniper
is known to possess significant insecticidal and repellence properties (Kanat and
Alma 2004; Gao et al. 2004). Against mosquitoes the Eos of Juniperus sp. is
reported to display potent repellent, insecticidal, oviposition-deterrent or larvicidal
properties (Prajapati et al. 2005; Amer and Mehlhorn 2006a, b). The mixture of the
leaves and cones of J. phoenicea is used as an oral hypoglycemic, whereas the
leaves are used against bronchopulmonary diseases and as a diuretic (Bellakhder
1997).
There are few reports on the composition of J. phoenicea leaf oil, even though
they concerned plants from various origins and a limited number of samples were
analysed every time. The first studies date back to 1956 and 1973 and reported
monoterpene-rich oils (subspecies not reported) (Gil de Meister and Hoffman 1956;
Banthorpe et al. 1973). More recently, Vidrich and Michelozzi (1993) reported
1,8-cineole, α-pinene and borneol as major components for an oil from Italy (sub-
species not reported). Adams et al. (1996) studied samples from different origins
and different subspecies. They reported the following compositions: α-pinene for J.
phoenicea (sensu stricto) from inland Greece and Spain and α-pinene/β--
phellandrene/α-terpinyl acetate for J. phoenicea var. turbinata from coastal Spain
and J. phoenicea subsp. eu-mediterranea from coastal Portugal. The composition of
50 samples of essential oil of individual plants of J. phoenicea subsp. turbinate from
Corsica was investigated by GC, GC–MS and 13C NMR. α-Pinene, β-phellandrene,
α-terpinyl acetate, Δ-3-carene, myrcene and α-phellandrene were found to be the
main constituents (Rezzi et al. 2001). Since species of J. phoenicea have already
been studied how the geographical situation affects the production of secondary
metabolites (Rezzi et al. 2001).
The essential oils of the leaves and cones have proven to possess antimicrobial
activity (Stassi et al. 1996). These results indicate that the EOs of Juniper plants
display potentials for use as natural alternative to synthetic agrochemicals, and
research is needed for the most potent EO identification and the thorough study of
its components (Vourlioti-Arapi et al. 2012). Hammami et al. (2009) mentioned that
J. phoenicea extract have the most active antibacterial activity in comparison with
two other plants from the arid regions of Tunisia; The essential oils, which were
utilised centuries ago in cosmetics usually, show interesting biological features. The
oils also help increase the flow of digestive fluids, improve digestion and eliminate
gas and stomach cramping (Uphof 1968). J. phoenicea has been used for centuries
as a steam inhalant for bronchitis and to control arthritis (Watt et al. 1962; Stassi
et al. 1996). According to the results presented by Hayouni et al. (2007) we can note
that extracts from J. phoenicea obtained by mixture: acetone/water/acetic acid
(90/9.5/0.5) showed more than 70% inhibition of scavenging of the ABTS radical,
which is as strong as the synthetic BHA. This could be attributed to the higher levels
of phenolics in J. phoenicea extracts. Juniperoside, a new 9-O(β-D-glucopyranoside)-
3,4,5-trimethoxycinnamyl alcohol has been isolated along with the 9-O-(α-L-
arabinofuranosyl-(1→6)-β-D-glucopyranoside)cinnamyl alcohol (rosarin) and
coumarin 7-O-β-D-glucopyranoside (skimmin) from the acetone extract of the aer-
ial parts of J. phoenicea (Comte et al. 1996).
Ephedraceae
Ephedra alata Decaisne, is a very effective sand-binder and resistant to desertifi-

cation (Abdallah and Chaieb 2007). Most of the 50 Ephedra species worldwide
(Stevenson 1993; Price 1996) are shrubs adapted to semiarid and desert conditions
(Pearson 1929). A few lianoid species are found in temperate regions along the
Mediterranean coastline and humid montane sites in North Africa and southwest
Asia (Freitag and Maier-Stolte 1989). About 25 species of Ephedra are found in the
drier regions of the Old World extending westwards from Central Asia across
southwest Asia and into Mediterranean Europe and North Africa (Freitag and
Maier-Stolte 1994). According to Boulos (1983), E. alata which grows wild in the
Egyptian desert provides extracts used in folk medicine as depurative, hypotensive,
anti-asthmatic, sympathomimetic and astringent agents. The branches are chewed
for cephalalgia, used in miscarriage and as a bronchodilator.
An Asian Ephedra species has been used for thousands of years in Traditional
Chinese Medicine herbal formulas designed to treat asthma and other respiratory
diseases (Rotblatt and Ziment 2002). According to Chen and Schmidt (1930) and
Bloedorn and Dickens (1928), Ephedra gained widespread medical use in the
United States in the 1920s as a nasal decongestant, central nervous system stimu-
lant, and asthma treatment, but use declined substantially in the following decade
because of safety concerns and the availability of safer alternatives. Many Ephedra-
containing products are claimed to be effective for weight-loss, as energy/perfor-
mance boosters, euphorics or aphrodisiacs, claims that are especially appealing to

young adults (Josefson 1995). Recently, Ephedra has become a common ingredient
in many dietary supplements that are promoted to increase energy or assist with
weight loss. Since herbs are regulated as dietary supplements in the United States,
these ephedra-containing products that can be sold without approval by the
U.S. Food and Drug Administration (FDA) (FDA 1994). Numerous case studies
have documented adverse effects in persons using ephedra (Haller and Benowitz
2000; Vahedi et al. 2000). In the largest published case series, Haller and Benowitz
(2000) reviewed 140 adverse event reports involving ephedra that were submitted to
the FDA. They concluded that 43 cases were definitely or probably related to ephe-
dra and another 44 were possibly related. However, there were certain limitations in
determining causality from case reports, the foremost of which is the inability to
determine relative risk.
Almost all commercial applications of Ephedra extracts derive from the ephed-
rine alkaloids found in the stems in many Eurasian species. The best-documented
drug made from Ephedra is Ma-huang. It contains several Ephedra alkaloids,
including the primary active constituent, ephedrine, as well as smaller amounts of
pseudoephedrine, phenylpropanolamine, methylephedrine, methylpseudoephed-
rine, and norpseudoephedrine (Gurley et al. 1998), used in Chinese medicine for
5000 year as a treatment for fever, nasal congestion, and asthma (Zhu 1998).
Ma-huang is also an effective respiratory sedative and cough remedy. These drugs
have also sympathomimetic activity and can lead to various physiologic responses,
including vasoconstriction; bronchodilation; and increases in blood pressure, heart
rate, cardiac contractile force, and automaticity (Hardman and Limbird 2001).
Herbal mixtures containing Ma-huang are sold in health food stores in the West as
nutritional supplements under such names as Herbal Ecstasy and Escalation (Gurley
et al. 1998) accompanied by dubious claims that they have energizing value or assist
in dieting (White et al. 1997). The organs used in traditional medicine are the dried
green stems, which are usually boiled in water and administered as a hot tea. The
usual daily dose is 1.5–9 g of the decocted herb (Leung 1999). The aerial parts of
different Ephedra species contain from 0.02% to 3.4% of 6 optically active alka-
loids concentrated in the internodes (Leung and Foster 1996). (−)-Ephedrine (EPH)
is the major isomer comprising 30–90% of the total alkaloids. It was the first alka-
loid isolated from Ephedra by Nagai in 1887. (+)-Pseudoephedrine (PSE), the dia-
stereomer of (−)-EPH, was subsequently isolated by Ladenburg and Ölschlägel in
1889 followed by the remaining isomers in the late 1920 (Ehab et al. 2003). In
addition to the ephedrine-type alkaloids, other alkaloids and amino compounds
have also been isolated from different species of Ephedra (Ehab et al. 2003).
The isolation of β-coumaric acid and (f)-2,6-bis-(3,5-dimethoxy-4-
hydroxyphenyl)-3,7- dioxabicyclo(3,3,0)octane (syringaresinol, 2) from E. alata
chloroform extract, the 2,3-digalloylglucopyranose (nilocitin, 3) from E. alata
ethanolic extract and the new alkaloid 7- methoxy-4-quinolone 2-carboxylic acid
(ephedralone, 4) from E. alata aqueous extract of the same plant were reported
(Nawwar et al. 1984). In addition to the known β-coumaric acid, the furanofuran
lignan (f)-syringaresinol and the digalloyl-glucose, nilocitin, were obtained from
the whole plant of E. alata. A new natural alkaloid, ephedralone, was also isolated
(Nawwar et al. 1985). Flavonol glucosides have been identified in E. alata, namely,
herbacetin methyl ether 3-0- glucoside-7-0-rutinoside and herbacetin 7-0-(6″-qui-
nylglucoside). The known flavonoids vicenin II, lucenin III, kaempferol
3-rhamnoside, quercetin 3-rhamnoside and herbacetin 7-glucoside were also found.
The structure of the isolated compounds was determined mostly by FABMS and
HNMR spectroscopy (Nawwar et al. 1984).
The pharmacology of Ephedra is complex, but for both traditional and more
recent popular uses, the established pharmacological effects appear to be attribut-
able to its ephedrine-type alkaloids, mainly (−)-EPH and (+)-PSE. Pharmacological
studies of Ephedra began shortly after ephedrine was first isolated and were focused
on the pure compound rather than on the whole herb extract (Takahashi and Miura
1888).
Globulariaceae
Globularia alypum L., is a perennial wild-growing shurb found throughout the

mediterranean area locally named as “Ain Larneb”. Its leaves are traditionally used
as hypoglycaemic agent, laxative (Bellakhdar et al. 1991), cholagogue, stomachic,
purgative and sudorific (Sijelmassi 1993). A recent ethnobotanical survey has dem-
onstrated that G. alypum is one of the most used medicinal plants in Fez region
(Jouad et al. 2001). Previous investigations of 624 patients suffering from diabetes
and/or hypertension in oriental Morocco have demonstrated that G. alypum repre-
sented the second highest percentage of medicinal plant utilization (9.9%) after
Trigonella foenum graecum (13.3%) (Ziyyat et al. 1997). Similar studies under-
taken in the Wilaya of Marrakesh have shown that this plant was the fifth highest
percentage (9.86%) of utilization (Jaouhari et al. 1999). Investigations were carried
out to evaluate the hypoglycaemic activity of the infusion of G. alypum leaves. Oral
and intraperitoneal administration of the plant (0.7 g/kg) produced a significant
hypoglycaemic effect in normal as well as in hyperglycaemic rats. The infusion
increased plasma insulin levels significantly in normal rats. It is suggested that the
hypoglycaemic activity of this plant may be mediated through enhancement of
peripheral metabolism of glucose and increase in insulin release (Skima et al. 1999).
A significant antileukemic activity of an aqueous extract of G. alypum was also
reported (Caldes et al. 1975).
The antioxidant activity of the G. alypum phytochemicals were evaluated by
Es-Safi et al. (2007) for their capacity to scavenge the 1,1-diphenyl-2-dipicrylhydrazyl
(DPPH) free radical and some structure–activity relationships were obtained. Assay
guided fractionation led to the isolation of syringin, four phenylethanoids, four fla-
vonoids and six iridoids as the main constituents of the extract and their antioxidant
activity was determined. The obtained results showed that the activity towards the
DPPH free radical was mainly due to the flavonoid and phenyl ethanoid constituents
which were most active free radical scavengers than iridoids. Their high antioxidant
activity could be attributed to the caffeoyl moieties contained in them, while iri-
doids showed moderate free radical scavenging activity. These results demonstrated
that some of the isolated compounds play an important role for the antioxidant
activity of G. alypum and give a scientific basis to the use of this plant in traditional
medicine. The hydromethanolic extract of G. alypum could thus be considered as a
source of potential antioxidants and will promote the reasonable use of this plant in
food technology and processing as well as for medical use.
G. alypum is used in the treatment of cardiovascular and renal diseases as dem-
onstrated by recent ethnobotanical surveys (Jouad et al. 2001; Jaouhari et al. 1999).
Recently, methanol and dichloromethane extracts of G. alypum were also shown to
reduce histamine and serotonin contraction in vitro (Bello et al. 2002). From the
hydromethanolic extract of the aerial parts of G. alypum grown in Morocco, a new
chlorinated iridoid glucoside, globularioside has been isolated beside 5 known iri-
doid glycosides, globularin, globularicisin, globularidin, globularinin and globulari-
min (Es-Safi et al. 2006).
Poaceae
Hyparrhenia hirta (L.) Stapf is a tufted perennial grass growing to 1 m in height
that is distributed mainly in the shore regions of the Mediterranean and across the
African continent (Clayton 1969). H. hirta Stapf occurs in Tunisia in higher densi-
ties in central and southern parts of the country (Chaieb and Boukhris 1998). H.
hirta is a pasture grass that has become highly invasive in several parts of the world,
including Australia where it has become a serious environmental weed in recent
decades. In Tunisia the plant is known for its diuretic properties (Boukef 1986).
The phytochemistry of H. hirta has been investigated until relatively recently,
when triterpenes, a β-ketone, aromatic aldehydes and some common flavonoids
were described (Ben Salah et al. 2000 and Bouaziz et al. 2001). Leaves of H. hirta
yielded the rare diastereoisomeric flavonolignans tricin 4′-O-(erythro-β-
guaiacylglyceryl) ether and tricin 4′-O-(threo-β-guaiacylglyceryl) ether together
with their 7-O-glucosides, which are the first flavonolignan glycosides to be isolated
as natural products (Bouaziz et al. 2002). Although the overall flavonoid profile of
H. hirta is typical of Poaceae in containing flavone C-glycosides and tricin
5-O-glucoside, the multitude of glycosides present in this species shows that the
exact glycosidic pattern of species may be of chemotaxonomic importance in the
family at lower levels of classification (Bouaziz et al. 2001). The methanol extract
of H. hirta show an anti-inflammatory (Hoult and Moroney 1994) and anti-oxidant
(Joyeux et al. 1995) activity, which could be attributed to the isolated flavonoids
(Bouaziz et al. 2001).
Labiatae (Lamiaceae)
Rosmarinus officinalis L. is a woody, perennial herb with fragrant evergreen

needle-like leaves. It is native to the Mediterranean region. Forms range from
upright to trailing; the upright forms can reach 1.5 m, rarely 2 m. The leaves are
evergreen, 2–4 cm long and 2–5 mm broad, green above, and white below with
dense short woolly hair. Flowering, very common in a mature and healthy speci-
men, usually appears in winter or spring and is variable in color, being white, pink,
purple, or blue. R. officinalis is very common in Europe, Asia and Africa, but only
in the areas around the Mediterraneean Sea and in many islands, particularly Sicily,
Sardinia, Corsica, Baleari and Elba (Pintore et al. 2002). In Tunisia, R. officinalis,
this is an interesting plant due to its essential oil, grows in mountains at an altitude
of 400–500 m and on plains but it is rare or absent in the islands (Djerba, Kerkanah).
In Tunisia, R. officinalis is present with an endemic variety (troglodytorum),
growing in the Matmatas region (southern Tunisia). It is characterized by its particu-
lar ecological adaptation to arid superior environmental factors (i.e. irregularity and
insufficiency of rainfall, low soil quality, etc.). Because of its rusticity, it grows in
every soil type, but it prefers a sandy, arid, calcareous, humus-poor soil. It can be
distinguished from the other varieties by its dark purple flowers, long stamens and
its greyish leaves and stem (Pottier-Alapetite 1981). R. officinalis is cultivated in
many family gardens in Tunisia as ornamental plant and to use its leaves in tradi-
tional medicine and for flavoring food. It is also cultivated for the valuable oil which
can be extracted from the flowering aerial tops, comprising leaves, twigs and flow-
ers, collected from spring to late autumn. Many studies have been interested in the
qualitative and quantitative variability of the composition, yield and biological char-
acteristics of the essential oil, due to intrinsic (genetics and plant age) or extrinsic
parameters such as climate and cultivation conditions or isolation methods (Bekkara
et al. 2007; Stevanovits-Banyai et al. 2003).
The fresh and dried leaves are used frequently in traditional Mediterranean cui-
sine as food flavoring and for medicinal purposes (Le Floc’h 1983). It contains a
great amount of essential oil (0.5–2.5% v/w), which is largely used in traditional
medicine as a pulmonary antiseptic, a choloretic and a colagoguic. It has alsosto-
machic, antidiarrhoic and antirhumatic properties (Oury 1984). It is also utilized in
the cosmetic industry producing various Cologne waters, bathing essence, hair
lotions and shampoos (Burt 2004). R. officinalis is known by its antioxidant proper-
ties (Raul et al. 2005; Sacchetti et al. 2005; Wang et al. 2008). The biological char-
acteristics of essential oil of R. officinalis have been studied by several authors. It
has been demonstrated that this oil exhibited antibacterial, antioxidant, insecticidal
and antimutagenic activities (Bannour et al. 2006; Gachkar et al. 2007; Moghtader
and Afzali 2009; Wang et al. 2008; Isman et al. 2008; Miresmailli et al. 2006).
Al-Sereitia et al. (1999) reported different pharmacological actions of R. officinalis
such as effects on central nervous system, on the circulation and on smooth muscle,
choleritic and antitumoriginic effect and antimycotic effect.
According to the geographic location of the species, in North Africa and in

Europe, the analyses of chemical composition showed that the species is mainly
represented by two major chemotypes, 1,8-cineole (over 40%) and α-pinene/cam-
phor (with equal ratio, 20-30%). The species is cultivated extensively in several
countries such as Portugal, Spain, Italy, in near orient, in Asia and in America.
This chemical composition of R. officinalis essential oil has been the subject of
considerable study. The reported components were mostly monoterpenes, the
majorones being α-pinene, 1,8-cineole and camphor, associated with variable
amounts of camphene, myrcene, limonene, borneol, verbenone, bornyl-acetate, etc.
The major types of R. officinalis can be distinguished with respect to these main
constituents: oils with over 40% of 1,8-cineole (oils from Morocco, Tunisia, Turkey,
Greece, Yugoslavia, Italy, France, Algeria) (Chalchat et al. 1993; Boutekedjiret
et al. 1998; Boutekedjiret et al. 1999; Rezzoug et al. 1998; Zaouali et al. 2005) and
oils with approximately equal ratios (20–30%) of 1,8-cineole, α-pinene and cam-
phor (oils from France, Spain, Italy, Greece, Tunisia, Algeria, South Africa)
(Domokos et al. 1997; Mangena and Muyima 1999; Mastelic and Kustrak 1997;
Tomei et al. 1995). One other chemical composition could be defined according to
the comparatively higher amount of myrcene in oils from Argentina, Portugal and
Spain (Mizrahi et al. 1991; Dellacassa et al. 1999). The literature also revealed some
other chemical compositions for rosemary oils. Oils dominated by camphor/1,8-
cineole/borneol from Cubaand Morocco (Pino et al. 1998; Elamrani et al. 2000), by
1,8-cineole/borneol/p-cymene from Turkey (Perez-Alonso et al. 1995), by
α-pinene/1,8-cineole/camphene from Iran and Morocoo (Elamrani et al. 2000;
Jamshidi et al. 2009), by α-pinene/camphor/α--pinene from Algeria, by1,8-cineole/
camphor/camphene/α-pinene from southern of Tunisia. Oils from Sardinia and
Corsica (Mediterranean islands) were characterized by its relatively high content of
verbenone (15.7–24.9%) with a considerable amount of α-pinene (13.7–24.6%) and
camphor (2.9–14.1%). Oils from Izmir and Canakkale (Turkey) were also relatively
rich in verbenone (4.4–45.2%) but with a considerable amount of 1, 8-cineole
(12.1–34.3%) and camphor (9.9–24.1%). The essential oils extracted by Clavenger
apparatus from leaves of R. officinalis cultivated in different areas in Djerba (Island
in the southern of Tunisia) were evaluated for their antioxidant (DPPH method) and
antibacterial activities (agar-well diffusion method) by (Akrout et al. 2010a, b). The
three essential oils chemotypes exhibited moderate antioxidant activity with an IC50
ranged from 4.186 mg/ml for chemotype I to 7.298 for chemotype II and showed
strong to moderate antibacterial activity with a MIC ranged from 0.156 to 1.25 mg/
mL (Chemotype I and chemotype II) and from 1.25 to 5 mg/mL (chemotype III).
The Thymus genus belonging to the Lamiaceae family includes approximately
350 species, existing mainly in Europe, Western Asia and the Mediterranean regions
(Mabberley 1997). Many species of Thymus have been widely used in folk medicine
in the world for their carminative, antispasmodic, emmenagogic and tonic proper-
ties (Bruneton 1993). In Tunisia, the genus Thymus includes four species: T. capita-
tus (L.) Hoffm. et Link., T. numidicus Poir., T. algeriensis Boiss. et Reut., and T.
vulgaris L. The species can be sympatric in a wide part of their distribution area.
The species Thymus algeriensis (Hyphodromi Section) is an endemic plant of

semi-arid and arid areas of Tunisia and Algeria (Pottier-Alapetite 1981; Le Floch
and Boulos 2008). In Tunisia, T. algeriensis populations are distributed from the
sub-humid to the lower arid climates at altitudes ranging from 120 to 1100 m. The
species grows on poor fertile calcareous soils and occurs in scattered, small popula-
tions, showing different levels of destruction, mainly due to overharvesting and
overgrazing. Close to 100 species have been identified in the kind of Thymus across
the world (Richard et al. 1985). They are reported to possess some biological effects
such as antispasmodic (Meister et al. 1999), antibacterial (Essawi and Srour 2000;
Dob et al. 2006), antifungal (Soliman and Badeaa 2002), anti-tabagism (Carlini
et al. 2006), giardicidal (Amaral et al. 2006), anticancer (Jaafari et al. 2007) and
antioxidant activities (Tepe et al. 2005). The First Edition of Brazilian Official
Pharmacopoeia, published in 1926, includes 1702 Monographs for medicinal prod-
ucts. Among those, two Monographs refer the thyme (Brandão et al. 2006).
Tunisian Thymus algeriensis Boiss & Reuter (Synonym T. hirtus Willd. subsp.
algeriensis Boiss. & Reut.) is known under vernacular name of “Mazoukcha”. In
traditional medicine, infusions, leaves and flowers decoctions were used as tonic,
anti-inflammatory, antiparasitic, antitussive and carminative (Stahl-Biskup and
Sáez 2002). It is an herbaceous fragrant plant largely used, fresh or dried, as a culi-
nary herb (Pottier-Alapetite 1981; Le Floch and Boulos 2008). Furthermore, this
plant is also widely used in folk medicine against illnesses of the digestive tube and
antiabortion (Le Floc’h 1983). T. algeriensis is used fresh or dried as a culinary herb
and in folk medicine for its antiseptic, antispasmodic and antifungal properties
(Hazzit et al. 2009; Giordani et al. 2008). In Algeria, they have been used as astrin-
gent, expectorant and cicatrising agents (Baba Aïssa 1991). The thyme has been
used, in Moroccan traditional medicine, in the treatment of diarrhoea, fever, cough,
infected areas and wounds. It was also used as a tonic and stimulant (Bellakhdar
1996; Sijelmassi 1993) and, generally, for its anti-inflammatory properties after
topical or oral administration (Ismaili et al. 2001, 2002, 2004).
Data on the essential oil composition of T. vulgaris of other continents have been
presented in numerous publications. Thyme oil is among the world’s top ten essen-
tial oils also used as a preservative for food purposes (Alçiçek 2011; Ehivet et al.
2011) and (Stahl-Biskup and Sáez 2002). The demand for essential oils from these
species is increasing for perfumery, cosmetic and medicinal uses (Hazzit et al.
2009). T. algeriensis are rich in essential oils and were characterized by a great
variability of both morphology and chemotypes (Stahl-Biskup 1991). There is obvi-
ously a chemical polymorphism of essential oils within the plants belonging to the
genus Thymus. In addition, the infraspecific variability of the essential oils in the
genus Thymus was also observed (Stahl-Biskup 1991). These differences observed
in the oil composition may be due to the collection time (development stage) and
ecological factors (Dob et al. 2006). Depending on the geographical origin and the
specific ecological sites from which it is collected, its quantitative and qualitative
composition vary greatly between countries and the diversity of essential oils arises
from the existence of many chemotypes (Hudaib et al. 2002; Owen et al. 2002;
Goodner et al. 2006; Snoussi et al. 2008). Even in the same country, genetic and
ecological factors, especially climatic, determine the distribution frequency of the

chemotypes among populations such as in Spain: thymol (Rota et al. 2008),
1.8-cineole and eucalyptol (Goodner et al. 2006) and in France (geraniol, linalol,
α-terpineol, thujanol-4, terpineol-4, thymol and carvacrol) (Granger and Passet
1973). Recently, investigations on the chemical composition of T. algeriensis essen-
tial oil wildly growing in Tunisia, allowed the identification of six chemotypes
according to the main compounds. In fact, a high variation among populations for
the majority of the compounds was shown (Ben El Hadj Ali et al. 2010). The varia-
tion of the essential-oil composition among 14 Tunisian natural populations of T.
algeriensis was assessed by GC (RI) and GC/MS. The populations were collected
from different geographical regions belonging to the sub-humid, upper semi-arid,
mean semi-arid, lower semi-arid, and upper-arid bioclimates. A total of 47 constitu-
ents, representing 81.0–96.5% of the total oil, were identified. The main volatiles at
the species level were 1,8-cineole (17.7%), α-pinene (15.5%), and camphor (8.2%).
A high variation among populations for the majority of the compounds was shown.
Camphor (0.2–14.0%), linalool (0.2–22.4%), borneol (<0.01–24.3%), caryophyl-
lene oxide (<0.01–18.8%), thymol (<0.01–54.9%), γ-terpinene (0.4–6.5%),
α-copaene (0.4–7.6%), linalyl acetate (<0.01–6.4%), and methyl eugenol (<0.01–
6.9%) were the main constituents differentiating the populations (Ben El Hadj Ali
et al. 2010).
Many studies on the antimicrobial activity (Zambonelli et al. 2004; Dorman and
Deans 2004) and antioxidative activity (Dorman and Deans 2004; Youdim et al.
2002) of these oils have been reported. On the other hand, several extracts of these
plants were tested for their pharmacological activity (Marti et al. 2005). Furthermore,
the oil was tested for antimicrobial activity against four bacteria, two fungi and two
yeasts. This oil exhibited a significant in vitro antimicrobial activity against Bacillus
subtilis (MIC = 0.5 lL/mL), as well as against all yeast and all filamentous fungi
tested (MIC = 0.5 and 1.0 lL/mL) (Dob et al. 2006). The antimicrobial properties of
the oil could be associated with the high percentage of linalool, which is known to
possess strong antimicrobial activities (Burt 2004; Pitarokili et al. 2002). In addi-
tion, it has been well known that the phenolic components of the essential oils
showed the strongest antimicrobial activity (Burt 2004). The essential oil activity is
then due either to minor constituents or a synergy between these and the major
components (Zakarya et al. 1993). Thymes are well known for their antispasmodic,
sedative, antioxidant (Mkaddem et al. 2011; Safaei-Ghomi et al. 2009) and
antiaflatoxigenic (Razzaghi-Abyaneh et al. 2009) properties. The T. algeriensis
essential oil was found to possess an interesting inhibitory activity towards ACE
with an IC50 value of 150 μg/ml. The obtained results also showed that this oil can
act as radical scavengers (IC50 = 0.8 mg/ml) and displayed a lipidperoxidation inhib-
itory activity (IC50 = 0.5 mg/ml) as evaluated by 2,2-diphenyl-1-picrylhydrazyl and
β-carotene bleaching methods, respectively. Previously isolated constituents flavo-
noids were: taxifolin, eriodictyol, 5,6-dihydroxy-7-3′,4′-trimethoxyflavone, and
5,6,4′-trihydroxy-7,3′-dimethoxyflavone (El-Domiaty et al. 1997).
Thymus vulgaris L., locally known “zaatar” and member of the Lamiaceae family,
is widely used in Mediterranean medicine for its biological activities (Biondi et al.
1993; Cosentino et al. 1999). T. vulgaris, also known as Spanish common thyme,
has its flowering period between March and July. It can be normally located from
sea level to 2000 m above sea level (Morales 1996). The buds of cloves known as a
dental analgesic, carminative, stimulant and antiseptic agent, are also used in the
traditional preparation of some Tunisian food dishes such us “couscous” due to their
flavouring activity (Snoussi et al. 2008).
T. vulgaris oil exhibited a high level of antimicrobial activities against Vibrio
spp. Strains compared to Mentha longifolia, M. pulegium, Eugenia caryophyllata
and Rosmarinus officinalis oil (Snoussi et al. 2008). As a valuable medicinal plant,
T. vulgaris possesses, antiseptic, antimicrobial, and antioxidative properties
(Dorman and Deans 2000; Nguyen et al. 2000; Dapkevicius et al. 2002).
Totally, 61 constituents were identified in T. vulgaris essential oil by capillary
GC and GC-MS. Thymol was the dominating compound in the all analyzed oils
(44.4–58.1%), followed by α-cymene (9.1–18.5%), α-terpinene (6.9–18.9%), and
carvacrol (2.4–4.2%). Phenolic compounds are the aroma principles in this chemo-
type of thyme. The quantitatively most important compounds are the phenols thy-
mol (44.4–58.1%) and carvacrol (2.4–4.2%), which constituted almost half of the
essential oil, followed by monoterpene hydrocarbons p-cymene (9.1–18.5%),
α-terpinene (16.1–18.9%), myrcene (1.5–2.2%), and β-thujene (1.0–1.5%). (E)-
Sabinene hydrate (0.7–1.2%), linalool (1.5–2.8%), and borneol (0.4–1.7%) were
dominant among monoterpenic alcohols (Baranauskiene et al. 2003).
Thymus capitatus (L.) Hoffm. et Link (Synonym Thymbra capitata (L.) Cav.
or Coridothymus capitatus Rch. f.) (Figueiredo et al. 2008).
It is widespread in the Mediterranean area. In Tunisia, populations grow on
sandy and often on rocky soils from the sub-humid to the upper arid bioclimates.
The annual rainfall of sites ranged from 300 to 1000 mm/year. Altitudes varied from
150 to 500 m (Nabli 1995). Populations were mainly associated with Ceratonia
siliqua L., Fumana thymifolia Spach, Hyparrhenia hirta Stapf., Olea europea L.,
Quercus coccifera L. and Rosmarinus officinalis L. It’s a perennial, herbaceous
shrub commonly used as a spicy herb and locally known under the common name
“Zaâtar”. T. capitatus is used as spicy herb for flavouring cheeses, soups, stews,
stuffings, meats, fishes, dressings, sauces, and honey. The essential oil of T. capita-
tus are also used in the flavour and food industries. The oil is used in the flavouring
of toothpaste, mouthwashes, cough suppressant medicines and in the manufacture
of perfumes and cosmetics. As a medicinal plant, T. capitatus has traditionally been
considered an anthelmintic, antispasmodic, carminative, emmenagogue, expecto-
rant, rubefactient, sedative, stimulant, and tonic. The plant has been used as a folk
medicine against asthma, arteriosclerosis, colic, bronchitis, coughs, diarrhea, and
rheumatism (Le Floc’h 1983). Nowadays, essential oils and their components are
gaining increasing interest because of their relatively safe status, their wide accep-
tance by consumers, and their exploitation for potential multi-purpose functional
use (Ormancey et al. 2001; Sawamura 2000). The essential oils of T. capitatus have
been investigated by many researchers whose reported that the major components
of these oils were thymol, carvacrol, linalool, γ-terpinene with the presence of
β-cymene, borneol and β-bisabolene in relatively lower amounts (Amirti et al.
2008a; Chaouch 2008a; Akrout 2004). T. capitatus, growing wild in the southern of
Tunisia, oil was mainly composed of carvacrol (68.8%) and β-cymène (11.1%)
(Bounatirou et al. 2007a). This chemical composition is in concordance with the
carvacrol chemotype growing in Tunisia (Bounatirou et al. 2007a, b; Akrout 2004;
Bounatirou et al. 2008), Morocco (El Ajjouri et al. 2008) and Greece (Karousou
et al. 2005). The Sardinian T. capitatus oil differed from Tunisian oil by its low
content of carvacrol (10.8%) and its high amount of thymol (29.3%) and β-cymene
(26.4%) (Cosentino et al. 1999). The Turkish oil is dominated by carvacrol (35.6%)
in addition with thymol (18.6%), β-cymene (26.4%) and γ-terpinene (12.3%)
(Goren et al. 2003). T. capitatus essential oils collected from the southern of Tunisia
exhibited antibacterial, antifungal and antioxidant activities (Bounatirou et al.
2007a, 2008; Amirti and Aberchane 2008; El Ajjouri et al. 2008). The determination
of the antiradical activity by DPPH method showed that T. capitatus oil exerted the
highest activity with (0.15 μl/ml), followed by the more commonly medicinal plant
used by local population in the southern parts of Tunisia for several purposes,
Artemisia herba-alba (1.0 μl/ml) and Artemisia campestris (2.09 μl/ml). The screen-
ing of the antibacterial activity against seven bacteria using the disc diffusion
method showed that T. capitatus oil strongly inhibited the growth of all bacteria
studied (20–30 mm) except Pseudomonas aerogunosa which was resistant to all
oils. These results show and confirm that T. capitatus possesses strong antiradical
and antibacterial activities, and therefore it could be used as a natural preservative
ingredient in food and/or pharmaceutical industries (Akrout et al. 2010a, b).
Ajuga iva (L.) Schreber
Several species of the genus Ajuga are used in African and Asian folk medicine.
Ajuga iva (L.) Schreber is an annual herbaceous plant, commonly found in the dif-
ferent regions of Morocco, where it is known as “Chendgora” (Ziyyat et al. 1997).
It is known for its numerous beneficial effects as a panacea (cure-all) (Hassar 1999),
specifically for gastrointestinal disorders (Bellakhdar et al. 1991), hypertension
(Ziyyat et al. 1997) diabetes (Ziyyat et al. 1997), and as an anthelmintic. In East
Africa, the plants of the genus Ajuga have been used as a remedy for fever, tooth-
ache, dysentery, and high blood pressure (Kokwaro 1976), and in the traditional
Chinese pharmacopoeia, they are known for their diuretic effect (Aliotta and Pollio
1994).
The plants of the genus Ajuga have been reported to have antifungal (Anonymous
2000; Kariba 2001), antibacterial (Chen et al. 1996; Anonymous 2000;
Bennaghmouch et al. 2001), antimycobacterial (Cantrell et al. 1999), antihyperten-
sive (Odek-Ogunde et al. 1993), antiplasmodial (Kuria et al. 2001, 2002), hypo-
glycaemic (El Hilaly and Lyoussi 2002), and larvae and insect antifeedant (Bondi
et al. 2000; Ben et al. 2000, Ben Jannet et al. 2001) activity. The empirical use of
Ajuga is corroborated by the isolation and identification of a number of the active
compounds, including antileukemic sterol glycosides (Akbay et al. 2002), hypogly-
caemicecdysteroids (Kutepova et al. 2001), antibacterial and insect antifeedant neo-

clerodane diterpenoids (Bremner et al. 1998; Ben et al. 2000; Bondi et al. 2000),
insect antifeedant diglyceride (Ben Jannet et al. 2001), vasoconstrictor
8-O-acetyharpagide (Breschi et al. 1992), and insect ecdysis inhibitors (Kubo et al.
1981). Some of the phytochemicals isolated from the Ajuga plantsare reported to be
cardiotonic (Kuria and Muriuki 1984), renal stimulant (Aliotta and Pollio 1994),
biliary sectretagogue (Syrov et al. 1986), antidote for liver toxicity (Syrov and
Khushbaktova 2001) and erythropoiesis stimulator (Syrov et al. 1997).
A. iva aqueous extract improves the antioxidant status by reducing lipid peroxi-
dation and enhancing the antioxidant enzymes activities in plasma, erythrocytes and
tissues of diabetic rat (Taleb-Senouci et al. 2009). An aqueous extract of the A. iva
whole plant showed hypolipidemic activity, in addition to its hypoglycemic effect in
normoglycemic and diabetic rats. In view of the hypoglycemic and hypolipidemic
activity, and its relatively non-toxic, A. iva may be a candidate for development as
an anti-diabetic agent in human (El-Hilaly et al. 2006).
Marrubium deserti De Noé
The genus Marrubium is represented by 97 species which are widely spread over
the temperate and warm regions. Marrubium species are indigenous in Europe, the
Mediterranean area and in Asia (Watt and Breyer-Brandwizk Watt and Breyer-
Brandwijk 1962). M. deserti is a common plant in Tunisia and is used in traditional
medicine in the form of a decoction as a remedy for asthma, diabetes and as a
diuretic. Furthermore, it is frequently used in traditional medicine for treating diges-
tive disorders as antispasmodic, and as local treatment against scorpion stings and
allergy (Maiza et al. 1993; Ould El Hadj et al. 2003). Recent research demonstrated
the antibacterial activity of M. deserti extracts (Edziri et al. 2007).
Marrubium species are reported in the special literature to be used in folk medi-
cine. As an example: aqueous and hydroalcoholic extracts of flowered aerial parts of
different Marrubium sp. are reported to treat cough and as choleretic, in digestive
and biliary complaints (Newall et al. 1996 and Wichtl and Anton Wichtel and Anton
1999). Traditionally, they are also used for their neurosedative and antiinflammatory
activities (Girre 2000).
The essential oil from aerial parts of M. deserti, obtained by hydrodistillation,
was analyzed by gas chromatography (GC) and gas chromatography-mass spec-
trometry (GC-MS). Thirty-seven compounds were identified in the oil, with
germacrene-D, as the major component (45.7%). This oil was characterized by a
significant hydrocarbon fraction (78.1%) and the predominance of sesquiterpenes
(67.4%) (Edziri et al. 2007).
The best known and first isolated diterpenoid was obtained from M. vulgare, in
1842. It was characterized as marrubiin. This furanic labdane diterpene is, in part,
responsible for therapeutic properties of Marrubium sp. (Stulzer et al. 2006). The
genus Marrubium is a rich source of diterpenoids, flavonoids and phenylethanoid
glycosides (Takeda et al. 2000; Rigano et al. 2006; Piozzi et al. 2006; Dendougui
et al. 2011).
Dichloromethane and methanolic extracts of M. deserti led to the isolation of

terpenoid among which the two new labdane diterpenes were named marrulibace-
tal-A and desertine, respectively. There were also six known compounds (a mixture
of the isomers cyllenin A and 15-epi-cyllenin A, marrubiin, marrulactone, marruli-
bacetal and β-stigmasterol) and seven known phenolic compounds were also iso-
lated: apigenin and several 7-O-substituted derivatives (apigenin-7-O-β-neohesperi
doside, apigenin-7-O-glucoside, terniflorin and apigenin-7-O-glucuronide) together
with two phenylethanoid glucosides (acteoside and forsythoside B). The structures
and relative configurations of the new compounds were elucidated by MS and a
series of 1D and 2D NMR analyses. Some pure compounds have been evaluated for
their antioxidant activities through different methods: DPPH and ABTS assays.
Genotoxic and antigenotoxic activities of extracts and pure compounds were also
evaluated in vitro on Escherichia coli PQ37 cells by the SOS Chromotest. Some of
the isolated compounds like phenylethanoid derivatives showed stronger antioxi-
dant capacity than trolox and were also able to significantly inhibit β-galactosidase
induction caused by the mutagen agent nitrofurantoin (Zaabat et al. 2011).
The antiviral activity of petroleum ether, ethyl acetate, chloroform, butanol and
methanol extracts of M. deserti was evaluated against human cytomegalovirus
(HCMV) strain AD-169 (ATCC Ref. VR 538) and Coxsackie B virus type 3 (CoxB-
3) using a cytopathic effect (CPE) reduction assay. The butanol, methanol and ethyl
acetate extracts showed significant antiviral activity against Coxsackie B3 virus
(Dziri et al. 2012).
Marrubium alysson L.
M. alysson is a perinial herb of the Lamiaceae family which is commonly distrib-
uted in Europe, Mediterranean, Asia (Mabberly 1997) and in Tunisia. Its flowering
aerial parts, as well as their aqueous and hydroalcoholic extracts are used in tradi-
tional medicine for treating cough but also as a laxative during digestive and biliary
complaints (Wichtel and Anton 1999).
M. alysson witch had an interesting antibacterial activity with MIC varied from
128 to 2000 μg/ml against different enterobacteriacea and had good antifungal
activity with MIC between 128 and 256 μg/ml (Edziri et al. 2007).
Teucrium polium L.
T. polium L. is a wild-growing flowering plant belonging to the Labiatae family and
is found abundantly in south western Asia, Europe and North Africa. Teucrium spe-
cies have been used as medicinal herbs for over 2000 years as diuretic, diaphoretic,
tonic, antipyretic, antispasmodic, an appetizer especially for children and chola-
gogic (Galati et al. 2000). An infusion of the leaves and flowers of the plant is con-
sumed as a refreshing beverage (Facciola 1990). The biological activities of T.
polium is widely reported and it has been shown to possess anti-inflammatory, anti-
nociceptive, anti-bacterial, anti-hypertensive, hypolipidemic, and anti-rheumatoid
effects (Rasekh et al. 2001; Abdollahi et al. 2003). The effect of ethanolic extract of
T. polium on carrageenan-induced acute inflammation, cotton-pellet granuloma and
some of the biochemical parameters were studied by Tariq et al. (1989). The etha-
nolic extract of T. polium at a dose of 500 mg/kg body weight produced significant
inhibition of carrageenan-induced inflammation and cotton-pellet granuloma. The

presence of flavonoids and sterols might be responsible for the anti-inflammatory
activity of this plant.
Two known phenylethanoid glycosides, verbascoside and poliumoside have been
reported from the aerial parts of T. polium (Bedir et al. 1999).
The crude extracts and isolated compounds were screened for their antioxidant
and free radical scavenging activities using DPPH radical-scavenging, beta-
carotene/linoleic acid and ammonium thiocyanate methods. Methanol extract, rutin
and apigenin were found to be the most active fractions as radical-scavengers with
CI50 values of 20.1 ± 1.7, 23.7 ± 1.9 and 30.3 ± 2.1 mg/ml, respectively. The samples
with the highest inhibition of oxidation of beta-carotene and lipid peroxidation in
ammonium thiocyanate methods were also found to be methanol extract, rutin and
apigenin. Methoxylated flavonoids exhibited a lesser antioxidant activity (Sharififar
et al. 2009). T. polium has a folk reputation, as a hypoglycemic agent. The hypogly-
cemic activity of an aqueous decoction of plant aerial parts was tested in normogly-
cemic and streptozotocin-hyperglycemic rats. Results indicate that this extract
caused significant reductions in blood glucose concentration 4 h after intravenous
administration and 24 h after intraperitoneal administration (Gharaibeh et al. 1988).
To assess the claimed hypoglycemic property of the crude drug and to get some-
knowledge about its mechanism of action. This extract was administered orally to a
group of streptozotocin diabetic rats for six consecutive weeks (0.5 g plant powder
per kg body weight). A significant decrease (64%) in blood glucose concentration
was observed in the treated animals compared to the untreated diabetic rats, without
any measurable effects on the major biochemical factors. In addition, the crude
extract significantly enhanced the blood insulin level by almost 160% compared to
the untreated diabetic rats. The insulinotropic property of the T. polium extract was
further assessed by an in vitro investigation using isolated pancreatic rat islets. The
data indicated that T. polium crude extract is able to enhance insulin secretion by
almost 135% after a single dose of plant extract (equivalent to 0.1 mg plant leaf
powder per ml of the culture medium) at high glucose concentration (16 mmol/l)
(Esmaeili and Yazdanparast 2004).
Some classes of constituents (diterpenoids, flavonoids, iridoids, sterols and ter-
penoids) was isolated (Carreiras et al. 1989; Hassan et al. 1979). Piozzi et al. (1987),
have isolated the previously known neo-clerodane diterpenoids 19-acetylgnaphalin,
eriocephalin, isoeriocephalin, and 3-deacetyl-20-epi-teulanigin (De la Torre et al.
1988), together with 5,3′,4′-trihydroxy-6,7-dimethoxyflavone (cirsiliol) (Harborne
et al. 1975) and 5,7,4′-trihydroxyflavone (apigenin) (Harborne et al. 1975). In addi-
tion, three new diterpenoids, teuvincentins A, B and C, have also been isolated from
the same source and their structures (2, 3 and 4, respectively) established by chemi-
cal and spectroscopic means and, in the case of teuvincentins A and B, by X-ray
diffraction analyses of their respective acetyl derivatives 6 and 10. From the aerial
parts of T. poliumsubsp. Vincentinum, three new neo-clerodane diterpenoids,
teuvincentins A, B and C, have been isolated besides four already known diterpenes
(19-acetylgnaphalin, eriocephalin, isoeriocephalin and 3-deacetyl-20-epi-teulani-
gin) and the flavones cirsiliol and apigenin. The structures of teuvincentin-A
((12S,20S)-20-0-acety1-19-acetoxy-18-ch1oro-15,16-epoxy-4~,7-dihydroxy-6-
oxo-neo-c1erodane-7,13(16),14-triene-20,12-hemiacetal), −B ((12S,20S)-20-0-
acetyl-4a,l8;15,16-diepoxy-6a-hydroxy-l7-neo-clerodane-13(16),14-diene-
7a,19; 20,12-dihemiacetal) and C ((12S,20S)-20-0-acetyl-6a,l9-diacetoxy-4a,l8;
15,16-diepoxy-7-oxo-17-neo-clerodane-13(16),14-diene-20,12-hemiacetal) were
established by chemical and spectroscopic means and, in the case of teuvincentins-
A and -B, also confirmed by X-ray diffraction analyses of their acetyl derivatives
(Carreiras et al. 1989).
Leguminosae/Papilionacées/Fabaceae
Astragalus gombiformis Pomel

Astragalus, genus of Fabaceae, comprises more than 1500 species that are distrib-
uted in the Orient and North Africa (Ozenda 1991). Many Astragalus species are
toxic. In contrast, several therapeutic properties are assigned to other plants of this
genus. In folk and modern medicine, Astragalus species such as A. monghalicus
Bunge and A. membranaceus (Fish) Bugne are used against various diseases and for
cancer therapy (Lei et al. 2003; Yesilada et al. 2005; Yin et al. 2006). Several species
of Astragalus are reported from the Tunisian flora, including A. gombiformis Pomel,
which grows in desert regions. In these areas, the plants normally synthesize several
metabolites in order to adapt to drought stress and are therefore a promising source
of active molecules A. Gombiformis is traditionally used against the bites of snakes
and scorpions (El-Rhaffari and Zaid 2002).
In Tunisia, the flora containes many Astragalus species such as A. epiglottis L.,
A. sesameus L., A. baeticus L., and A. caprinus usually distributed in desert regions
(Le Floc’h 1993). Except the studies by Semmar et al. (2001, 2002, 2005) on A.
caprinus, no previous phytochemical investigations have been reported on
Astragalus species of Tunisian flora. A. gombiformis Pomel, syn. A. gombo subsp.
gombiformis (Pomel) Ott, has a narrow spread in the big oriental Erg of Tunisia
under Saharan climate characterized by different stresses. Although, this plant has a
significant biomass, it is only moderately edible for animals, probably due to its
toxicity or its rankness (Teyeb et al. 2012).
The main chemical compounds of A. gombiformis are: 6,10,14-trimethylpentade
can-2-one, hexadecanoic acid, phytol and dillapiole. Many volatiles of interest are
identified such as butylated hydroxytoluene (BHT), an antioxidant compound.
Other compounds which can be responsible for toxicity are identified with different
amounts between organs such as caprolactam and benzencacetonitrile (Teyeb et al.
2010). A. gombiformis essential oils are complex mixtures of important natural
compounds, which varied qualitatively and quantitatively between cultivated and
wild plants and between phenological stages of development. All analysed oils are
characterized by the constant presence of phytol, 6,10,14-trimethyl-2-pentadecanone,
4-terpinol, and γ-terpinene (Teyeb et al. 2011). The antibacterial and cytotoxic
activities of extracts from the leaves of wild A. gombiformiswere reported. The
strongest cytotoxic activity against the human A549 lung epithelial carcinoma cell
line was detected for dichloromethane extracts at IC50 = 85 ± 21.7 ug/ml. The meth-
anol extract was found to be the most active against Pseudomonas aeruginosa and
Salmonella typhimurium (Teyeb et al. 2012). In Turkish folk medicine, the roots of
Astragalus species are used for the treatment of leukemia and for the healing of
wounds (Yesilada et al. 2005). Some Astragalus products, such as gum tragacanth,
are widely in use in the preparation of pharmaceuticals and as thickening agents in
certain foods (Zarre 2000). Some products may even have applications in control-
ling cancer cells (Somer and Calışkan 2007).
Calicotome villosa (Poir.) Link. is a spiny shrub, with yellow papilionaceous flow-
ers during the spring season (Pignatti 1982; Tutin 1972), very common in the
Mediterranean area, where it grows near the sea (0–1200-m altitude). C. villosa was
considered as an antitumoral agent (Hartwell 1982), while in the Sicilian folk medi-
cine it was used for the treatment of foruncle, cutaneous abscess and chilblain
(Lentini et al. 1993). According to Dessi et al. (2001), the methanol extract and
essential oil of this species exibit an antioxidant, cytotoxic and antimicrobial activi-
ties. Previously isolated constituents in C. villosa, isoflavones (Harborne 1969),
alkaloids and chrysin (Dessí et al. 2001). Two new acylated flavone glycosides,
chrysin 7-(6″-O-acetyl)-O-β-d-glucopyranoside (1) and chrysin 7-(4″-O-acetyl)-O-
β-d-glucopyranoside (2) were isolated from the aerial parts of C. villosa, along with
17 known flavonoids and one triterpene (Pistelli et al. 2003).
Genista saharae Coss. et Dar., growing in Sahara, is a spontaneous shrub legume.
This evergreen legume is well adapted to drought stress and it is a goodcontributor
to dune stabilization (Mahdhi et al. 2007). According to ethnobotanical investiga-
tion, arial parts of G. saharae are traditionally used for treating respiratory diseases
and possess diuretic property (Mekkiou et al. 2005). A good antioxidant capacity
(26.5 mg of Vitamin C Equivalent/g plant extract and antibacterial activity against:
Escherichia coli (ATCC 25922), Staphylococcus aureus (ATCC 25923) were shown
by ethyl acetate fractions (EAF) of G. saharae (Bouchouka et al. 2012). The ethyl
acetate fraction (EAF) of G. sahara was found to have the highest content of both
total phenolic compounds and total flavonoids with 459.28 mg GAE and 242 mg
QuE per g of plant extract, respectively (Bouchouka et al. 2012).
Recently, the scientific community got interested in the studies involving G. sah-
arae (Mekkiou et al. 2005; Boumaza et al. 2006). According to these authors, G.
saharae, like other species of Fabaceae family, contains high levels of isoflavones,
notably C-glycosylated isoflavones.
Retama raetam (Forsk.) Webb. Locally named as “R’tm”, is a wild plant common
to the north and east Mediterranean regions (Mittler et al. 2001). The plant flowers
from April to May. It is used for traditional treatment of some renal diseases and for
treatment of hypertension (Kassem et al. 2000; Burits and Bucar 2000). Since it was
reported to show significant diuretic activity (Caceres et al. 1987). This specieshas
been used since ancient times as a herbal remedy for traditional treatment of wounds,
bites of snakes and sheep scabies. Branches are used as febrifuge; powdered
branches mixed with honey are emetic, given as a purgative and vermifuge, abortive
in large doses. The plant is used for making eye wash for eye troubles. The antibac-
terial, antifungal, antioxidant and antiviral activities were also recent reprted by
Edziri et al. (2008). Edziri et al. (2008) added that among the different extracts stud-
ied by the butanol extract of R. raetam showed high antimicrobial activity against
Gram-positive bacteria than the other extracts. It has MICs of 0.256–0.512 mg/ml
against Bacillus subtilis, Enterococcus faecium, Streptococcus agalactiae,
Streptococcus pyogenes, Corynebacterium spp, MSSA, and MRSA. Ethyl acetate
and butanol R. raetam extracts displayed more important antibacterial activity
against Gram-positive bacteria than Gram-negative bacteria.
The screening of chemical groups in the methanol extract of the flowers of R.
raetamcollected from Tunisia revealed the presence of alkaloids, flavonoids, and
tannins (Edziri et al. 2008). The total phenolic contents varied from 135.91 to
220.81 mg CAE/g in R. raetam butanolic extracts which exeded the total phenolic
contents of other extracts (Edziri et al. 2008). Flavonoid content of the extracts of R.
raetam flowers ranged from 41.58 to 54.97 mg CAE/g. The major components in
the R. raetam oil detected were nonanal (35.75%), humulene (29.29%), acetalde-
hyde (7.84%), linalool (5.62%), myrcene (3.38%), tridecanal (2.21%), caryophyl-
lene (1.79%), terpinyl acetate (1.46%), terpinolene (1.26%) and methyl anthranilate
(1.06%) (Edziri et al. 2010). Retama species have been reported to contain alkaloids
(Abdel Halim et al. 1997) and flavonoids such as daidzein, vicenin-2, naringenin,
apigenin, kaempferol, quercetin and kaempferol-7-O-glucoside in the seeds, and
daidzein 7, 4′-dimethyl ether, chrysoeriol 7-O-glucoside and orientin in the leaves
(Abdalla and Saleh 1983). Kassem et al. (2000) have isolated two new flavonoids
from the aerial part: luteolin 4′-O-Neohesperidoside and 5, 4′-dihydroxy-(3″,
4″-dihydroxy)-2″, 2″-dimethylpyrano-(5″, 6″: 7, 8)-flavone.
Ceratonia siliqua L.
Ceratonia siliqua L., commonly known as carob, is distrubuted in the Mediterranean
region (Polunin and Huxley 1972). The nonfleshy and bean-like fruits, called “carob
pods”, are a traditional part of the diet in the Mediterranean region, and the plant has
been cultivated in the region for centuries for its edible fruits (Ayaz et al. 2007).
Current world production of carob pod has been estimated at about 310,000 tons per
year, produced from about 200,000 ha with very variable yields depending on the
cultivar, region, and farming practices (Makris and Kefalas 2004). Its bark and
leaves are used in Turkish folk medicine as an antidiarrheal and diuretic (Polunin
and Huxley 1972; Baytop 1984). The fruits are traditionally used as an antitussive
and against warts (Merzouki et al. 1997, Amico and Sorce 1997). Its hormone-like
effects have been reported. Carob pods have been consumed traditionally as animal
and human food (Binder et al. 1959). Industrially, carob is mainly used for locust
bean gum production from the seeds. From the pulp, different human foods can be
derived, such as sugar syrups or molasses, unroasted and roasted carob powder used
as cocoa substitutes, or especially tannin-rich preparations as antidiarrheic products
(Marakis 1996; Loeb et al. 1989). Carob pulp products are especially rich in insol-
uble dietary fiber (carob fiber) that has recently shown to have promising cholesterol-
lowering properties both in animals (Pérez-Olleros et al. 1999a, b) and human trials
(Zunft et al. 2001; Zunft et al. 2003). Furthermore, carob fiber products, as well as
water extracts from carob pods, showed a high antioxidative activity in in vitro tests
(Haber 2002; Kumazawa et al. 2002). Results from the antimicrobial screening of
ethanol extract of C. siliqua inhibited the growth of five out of the ten microorgan-
isms studied and showed that ethyl-acetate and n-hexane extracts of C. siliqua were
more active against Enterococcus faecalis ATCC 29212 than Ceftazidime.
Carob pods contain condensed tannins (proanthocyanidins), composed of flavan-
3-ol groups and their galloyl esters (Bravo et al. 1994; Marakis et al. 1997), gallic
acid (Corsi et al. 2002), (+)-catechin, (−)-epicatechin gallate, (−)-epigallocatechin
gallate, and quercetin glycosides (Sakakibara et al. 2003). Carob pod meal con-
tained high levels of carbohydrates (45%), appreciable amounts of protein (3%),
and low levels of fat (0.6%). Germ and seedmeal contained more fat and less carbo-
hydrates as compared to the pods. Carob pods contain a mean value of 19 mg of
total polyphenols/g, 2.75 mg of condensed tannins (proanthocyanidins)/g, 0.95 mg
of hydrolysable tannins (gallo- and ellagitannins)/g. Germs contained higher con-
centrations of total polyphenols (40.8 mg/g) and tannins (16.2 mg ofcondensed
tannins/g and 2.98 mg of hydrolysable tannins/g) while only traces of these com-
pounds were detected in carob seed (Avallone et al. 1997).
Carob meal contains, among total polyphenols, a high level of pro-anthocyanidins
in comparison to hydrolysable ones represented by ellagitannins and gallotannins
(Avallone et al. 1997). Extracts from pods and leaves of carob were tested for their
abilityto inhibit cell proliferation of mouse hepatocellular carcinoma cell line (T1).
The two extractsshowed a marked alteration of T1 cell proliferation in a dose-related
fashion reaching themaximal effect at 1 mg/ml. Moreover, we demonstrated that
leaf and pod extracts were able to induce apoptosis in T1 cell lines after 24-h treat-
ment mediating a direct activation of the caspase 3 pathway (Corsi et al. 2002).
HPLC analysis revealed the presence of gallic acid, (y) epigallocatechin-3-gallate
and (y) epicatechin-3-gallate in pod and leaf extracts, compounds well known to
exert antiproliferative effects. The discovery that carob pod and leaf extracts con-
tained antiproliferative agents could be of practical importance in the development
of functional foods and/or chemopreventive drugs (Corsi et al. 2002).
Genista microcephala Coss et Dur. is an endemic plant from North Africa that
flourishes from May to July (Quezel and Santa 1962). G. microcephala is distrib-
uted in Eastern Algeria (Aurès, Hauts-Plateaux) and Tunisia (Quezel and Santa
1962; Maire 1987). G. microcephala var. tripolitana (Bornm.) Maire occurs only in
Libya (Tripolitania) and South-Eastern Tunisia (Maire 1987).
The Genista species show interesting biological properties such as hypoglyce-
mic, antiinflammatory, anticuler, spasmolytic, antioxidant, estrogenic and cytotoxic
activity against different human cancer cell lines (Scarpato et al. 2008; Rauter et al.
2009; Rigano et al. 2009). Phytochemical analysis of G. microcephala has revealed
the presence of flavorous, isoflavones and alkaloids (Martins et al. 2005; Tosun et al.
2009). An acceptable yield, 0.2% is obtained 27 compounds were identified by
Lograda et al. (2012) representing 98.9% of the total essential oil. The oils showed
significant antibacterial activities against Echerchia coli, Pseudomonas agreunisa
and Staphylocus aureus. A chemical investigation of the aerial parts of G. micro-
cephala, yielded two alkaloids, identified as lupanine (2-oxosparteine) and

S-calycotomine. The complete 1H and 13C chemical shifts assignments of Lupanine
and Scalycotomine were determined using 1D and 2D NMR spectroscopy. The
alkaloid extract of G. microcephala was tested against bacteria and fungi strains,
which showed a strong sensitivity (Zellagui et al. 2004).
Poaceae
Cymbopogon schoenanthus (L.) Spreng, is an aromatic herb known in Tunisia by

the name of “El bekhirai”. Fresh young leaves are consumed in salads and are used
to prepare traditional meat recipes. Due to its pleasant aroma and taste it is used to
prepare an aromatic “tea” that is much appreciated and largely consumed in the
north of Africa (IUCN 2005). Besides its culinary use, C. schoenanthus is also used
in folk medicine. Its medicinal properties are known from the antiquity, being
already described by “Pliny the Eldey” in his book Naturalis Historia (Pline
L’Ancien 1848–1850). Le Floc’h (1983) reports its use for the treatment of rheuma-
tism and fever. This author describes also its use as a diuretic, insecticide and a
poultice to cure dromedary wounds.
C. schoenanthus or camel grass is a major essential oil bearing aromatic grass
that grows well in arid areas. Essential oils obtained from the aerial parts of plants
are used extensively in perfumery and pharmaceutical industries. C. schoenanthus
oil has been reported to be severely toxic for some parasite species. The oil is used
to reduce density of parasitoid populations and increase seed losses (Ketoh et al.
2002). The chemical composition, antioxidant activities and acetylcholinesterase
inhibitory properties of the essential oils from fresh leaves, dried leaves and roots
collected from three different locations in southern Tunisia, were evaluated by
Khadri et al. (2008). Essential oils were analysed by GC–mass spectrometry and
13C NMR. The major components were limonene (10.5–27.3%), β-phellandrene
(8.2–16.3%), α-terpinene (4.3–21.2%) and α -terpineol (6.8–11.0%) (Khadri et al.
2008). Antioxidant, acetylcholine-esterase activity of C. schoenanthus essential oil
was reported by Khadri et al. (2008, 2010). C. schoenanthus essential oil was active
against both termites (Koba et al. 2007) and the bruchid (Callosobruchus macula-
tus), which is a major pest of stored grains (Ketoh et al. 2002).
Polygonaceae
Calligonum genus belongs to the Polygonaceae family (Wang 2007), with some 80
species distributed throughout Western Asia, Southern Europe and North Africa.
Only C. comosum L’Hérit, C. azel Maire and C. arich Le Houérou are represented
in the Tunisian arid zone and the last one is endemic (Le Houérou 1959). The three
Calligonum species are dominant perennials in active sand dunes and stabilized
sand fields in the southern desert of Tunisia and grow naturally in the eastern Great
Erg (Le Houérou 1959). Several species have a C4-type of photosynthesis and can
grow in mobile sand dunes, under extreme drought conditions (Ren 2001). Due to
their high tolerance of water deficits, these speceies appear to be suitable for re-
vegetating deserts (Dhief et al. 2009).
Calligonum comsum Hérit, is widely distributed, from the North African deserts to
the desert sands of the Middle East and as far east as the Rajputana desert in western
India (Le Houérou 1959).
In the Tunisian desert, C. comosum was used for the treatment of the scabies of
the camel. The decoction of roots of this species was anthelmintic. In theUnited
Arab Emirates, C. comosum is used by some healers to treat stomach ailments, the
stems and leaves are chewed for curing toothache. The fresh aerial parts of this spe-
cies, collected in Abu Dhabi Emirates, show an anti-inflammatory and antiulcer
activity. Some compounds, extracted from Egyptian C. comosum, presented cyto-
toxic and antioxidant activity. Calligonum species are known to contain high levels
and various types of polysaccharides, secondary metabolites, polyphenols, alka-
loids, flavonoids, and tannins (Liu et al. 2001). Dhief et al. (2011) studied the essen-
tial oils of the three wild species of Calligonum growing in the Tunisian desert. The
oils obtained by hydrodistillation were analyzed by GC-FID and GC-MS in order to
compare their chemical composition. A total of 110 compounds, which accounted
for 94.0–99.7% of the total composition of the essential oils, have been identified.
The main constituents were viridiflorol (9.6%) in C. azel, hexadecanoic acid
(20.1%) in C. arich and 9-octadecenoicacid (19.8%) in C. comosum. Based on their
chemical composition, two chemotaxonomic groups are proposed: C. azel on one
hand, C. arich and C. comosum.
Polygonum equisetiforme Sibth. et Smis
In the south of Tunisia, Polygonum equisetiforme Sibth. et Smis is used for the dis-
infection of wounds, healing and fortifying. It is also used to treat cough, cold and
sore throat (Khafagi and Dewedar 2000). Crude extracts of P. equisetiforme, as well
as the pure compounds isolated from them, were screened for antibacterial and anti-
fungal activity against 20 bacterial and 17 fungal cultures. The crude extract of P.
equisetiforme inhibited the growth of Trichophyton mentagrophytes, M. canis and
Allescheria boydiiat an MIC of 450–500 μg/ml whereas the pure compounds inhib-
ited the growth at MIC of 300–350 μl. Species of Corynebacterium, Bacillus and
Staphylococcus aureus were found to be highly susceptible to the crude and the pure
compounds. MIC values of both crude extracts for different organisms were found
to be higher (200–350 μg/ml) than those of the pure compounds (150–170 μg/ml).
The crude extract of P. equisetiforme did not inhibit the growth of P. aeruginosa,
however, the pure compound was found to be bacteriostatic (Kazmi et al. 1991).
Quercetin (a flavonol) has been isolated and identified as the major constituent of P.
equisetiforme (Ghazal et al. 1992).
Rhamnaceae
The genus Ziziphus belongs to the family Rhamnaceae and is represented by 135–
170 species (Liu and Cheng 1994). These are evergreen or deciduous trees, or
shrubs, usually armed with unequal stipular spines. Of these, only Z. spina-christi
(L.) Willd, Z. vulgaris Lam. and Z. lotus (L.) Lam. are found in Tunisia (Laamouri
and Zine El Abidine 2000). Although highly similar, Z. lotus differs from the other
two species by its deciduous shrubby habit with intricately branched stems and
smaller flowers and fruits (Jafri 1977). This species is indigenous to Tunisia. It
grows under a variety of environmental conditions and has a wide ecological and
geographical distribution in both climatic regions of Tunisia. It is a shrub that
reaches 2–5 m in height and is found in depressions with deep sandy soil. Mounds
composed of wind-borne sediments that accumulate around Z. lotus thorn scrub
have long been reported from the Tunisian steppe regions (Tengberg and Chen
1998). This species is a dominant perennial shrub in both the active sand dunes and
stabilized sand fields of the southern, arid zone of Tunisia. Z. lotus is dormant from
October through March and mature plants flower in May–June and produce fruits in
August.
Ziziphus lotus (L.) Desf. is abundantly present in the Mediterranean region,
throughout Libya to Morocco, Algeria and southern European countries like Spain,
Sicily, Greece and Cyprus (Gorai et al. 2010). The North African species of the
genus Zizyphus are known with the vernacular names ‘sedra’, ‘addhal’, ‘roubaidh’,
‘dhou achaouk’, ‘sder alberri’ and ‘cder, nabga’ (Marie 1964; Boukef 1986). Fruits,
leaves, seeds, roots and bark of jujube have been commonly used as a source of
simple medicines in traditional medicine (Le Croueour et al. 2002; Abdel-Zaher
et al. 2005; Li et al. 2005). Flowers of Chinese jujube have high-quality nectar, and
the leaves are consumed as tea (Zhao et al. 2008). The fruit is the edible part of the
plant by local population. Several parts of Zizyphus have been used by traditional
and ancestral medicine, both in North Africa and Middle East, for the treatment of
several pathologic conditions including digestive disorders, weakness, liver
complaints, obesity, urinary troubles, diabetes, skin infections, fever, diarrhoea and
fatigue, and distress, which cause insomnia (Boukef 1986; Adzu et al. 2003; Lahlou
et al. 2002). Z. lotus are used is emollient (Le Floc’h 1983). The Ethnomedicinal
evaluation of this plant in Tunisia by Le Floc’h (1983) indicates that seeds and flow-
ers have sedative effect. They can contribute to the overall tonification therapy, nor-
malize the function of stomach, calm the mind and treat eyes inflammation. They
are reported to be effective in treating vitamin B deficiencies. The fruits are known
to be beneficial to lose weight and as such, they are used in treating cases of obesity.
Farther uses are: spleen tonic, aphrodisiac, blood cleaning, appetizer. The dried
fruits have been reported to facilitate digestion (Boukef 1986). Zizyphus is also used
for treatment of fever, skin problems and for fortifying hair (Le Floc’h 1983).
Jujube fruits are consumed fresh, dried and processed (jams, loaf, cakes, jelly,
etc.) throughout the world (Pareek 2002). Fruits have a high sugar content and high
levels of vitamin A, C and B complexes, phosphorus and calcium (Pareek 2002).
Alkaloids, flavonoids, sterols, tannins, saponin, and fatty acids have been detected
in the different species of the genus Ziziphus (Le Croueour et al. 2002; Abdel-Zaher
et al. 2005; Bhargava et al. 2005; Zhao et al. 2006). Z. lotus extracts contain anti-
spasmodic constituents mediating their effect through cholinergic receptors and
blockade Ca2+ influx. This could explain the traditional use of Z. lotusin the treat-
ment of the intestinal diseases (Borgi et al. 2007). The different extracts of Z. lotus
exerted immune-suppressive on T-cell proliferation and IL-2 mRNA expression and
antioxidant effects. Borgi et al. (2007) have shown that root barks of Z. lotus, given
intraperitoneally, showed a significant and dose-dependent anti-inflammatory and
analgesic activity in carrageenan-induced paw edema in the rat. Hence, the presence
of flavonoids in the Zizyphus extracts was supposed to be responsible for these ben-
eficial effects. Adhvaryu et al. (2007) have shown that Zizyphus extracts used with
other plants stimulated neutrophil functions and exerted hepatotoxic and immuno-
modulatory effects in guinea pigs. Chan et al. (2006) assessed cell signaling mecha-
nisms in T-cells and provided the evidence that a mixture of herbs containing
Zizyphus extract induced the expression of mitogen-activated protein kinases (i.e.
ERK, JNK and p38) in T-cells, indicating that the immunomodulatory effects of
Zizyphus involve the activation of second messenger cascade. Since Z. lotus has
been shown to modulate different disorders (Adzu et al. 2003; Han and Park 1986).
Four cyclopeptide alkaloids, lotusines B, C, E and F, have been isolated from the
root bark of Z. lotus. Their structures were elucidated mainly by homo- and hetero-
nuclear NMR techniques. Besides, several biologically active molecules, particu-
larly cyclopeptide alkaloids, termed lotusiones (Ghedira et al. 1993; Le Croueour
et al. 2002) and dammarane saponins have been isolated from this shrub (Renault
et al. 1997). Cyclopeptides extracted from Z. lotus exhibited antibacterial and anti-
fungal properties (Pandey et al. 1990). Five dammarane-type saponins were isolated
by means of centrifugal partition chromatography from the leaves of Z. lotus. Their
structures were elucidated using a combination of 1D and 2D 1H and 13C NMR
spectra and mass spectroscopy. One of these glycosides is the known jujuboside B
(5). Three are new jujubogenin glycosides, identified as 3-O-α-L-rhamnopyranosyl-
(1_6)-β-D-glucopyranosyljujubogenin-20-O-(2,3,4-O-triacetyl)-α-L-rhamnopyranoside
(1), 3-O- α -L-rhamnopyranosyl-(1_6)- β -D-glucopyranosyljujubogenin-20-O- α
-L-rhamnopyranoside (2), and 3-O- α -L-rhamnopyranosyl-(1_2)-((4-sulfo)- β
Dglucopyranosyl-(1_3))- α -L-arabinopyranosyljujubogenin (3). The last is a new
sulfated derivative of jujubasaponine IV, identified as 3-O-R-L-rhamnopyranosyl-
(1_2)-((4-sulfo)- β -D-glucopyranosyl-(1–3))- β -D-galactopyranosyl-(20R,22R)-16
β,22:16R,30-diepoxydammar-24-ene-3 β,20-diol (4) (Borgi et al. 2007). Catechin,
caffeic acid, p-coumaric acid, ferulicacid, rutin, apigenin-7-glucoside, eriodictyol,
quercetin, p-hydroxybenzoic acid, chlorogenic acid andsyringic acid were isolated
from leaves were analyzed withan HPLC device equipped with a diode array detec-
tor, and the fatty acid methyl esters were analyzedwith QP 5050 GC/MS equipped
with a CP-Wax 52 CB column. Rutin content was very high in the leaves of all the
jujubeselections, ranging from 269.0 to 367.90 mg/100 g, followed by apigenin-7-
glucoside (22.90–49.38 mg/100 g) and eriodictyol (5.06–6.27 mg/100 g). Seven
phenolic compounds, catechin, caffeic acid, epicatechin, ferulic acid, rutin,
p-hydroxybenzoic acid and chlorogenic acid, were isolated from fruits of jujube
selections. Catechin level ranged from 2.46 to 3.74 mg/100 g, and rutin level ranged
from 0.88 to 3.60 mg/100 g for fruits (Borgi et al. 2007).
The predominant fatty acids in all jujube selections were oleic acid, linoleic acid,
palmitic acid andpalmitoleic acid. Unsaturated fatty acids comprised 68.54–72.44%
of the total fat in jujube fruit.
Rutaceae
Ruta graveolens L. (chalepensis L. or montana)

The most common medicinal plant of this family is Ruta graveolens, known as rue
and native to Europe. The plant is now available all over the world, though prefer-
ably grown in Mediterranean climates. This plant is in medicinal use for various
clinical conditions from very ancient time but rationality of its use is still controver-
sial. R. graveolens is a medicinal plant which has been traditionally used as a seda-
tive and antihelmintic to relievemenstrual and gastrointestinal disorders (Guarrera
1999; Skidmore-Roth 2001), hypotensive, (Trovato et al. 2000) and antifertility
(Al-Okbi et al. 2002). In homeopathy, rue is an important remedy for deep aching
pain and rheumatism besides being used for eyestrain-induced headache. It has also
been used as a remedy for gastric disorders, stiff neck, dizziness and headache
(Conway and Slocumb 1979). R. graveolens L.has been notorious, since at least the
first century of the Christian era, for its ability to produce erythema and pustular
eruptions on human skin coming in contact with it. In the present century, the lesions
were characterized as a photophytodermatitis induced by exposure to UV and due
to the presence in this species of linear furanocoumarins, psoralen and some of its
derivatives (Murray et al. 1982).
R. graveolens is a medicinal plant, used since time immemorial. Traditionally
Rue is considered to be emmenagogue, ecbolic, anthelminthic and antispasmodic. It
has been approved by Food and Drug Administration (FDA) as a flavouring agent.
Antiinflammatory (Raghav et al. 2006) anti fungal (Oliva et al. 2003) antibacterial
(Ojala et al. 2000) and hypotensive (Chiu and Fung 1997) activities of R. graveolens
have been scientifically proven but at higher concentrations it has been reported to
exhibit toxicity (El Agraa et al. 2002). Most of these biological properties that have
beendenoted by preceding studies are likely related to some chemical constituents
including flavonoids (Chen et al. 2001; Chiu and Fung 1997). The most important
analyzed flavonoid in R. graveolens L. is rutin (quercetin-3-O- b-rutinoside) that
belongs to flavonol glycoside. Quercetin is another major flavonoid found in R.
graveolens L. and can also be obtained by rutin hydrolysis. The purpurted therapeu-
tic effects of many traditional medicines have been attributed to flavonoids in par-
ticular as a result of their enzyme inhibitory and antioxidant activity (Havsteen
1983; Chiu and Fung 1997). R. graveolens plant contains approximately 2% of
rutin. An interesting recent study reported the decrease in lipopolysaccharide-(LPS)
induced nitric oxide production by rutin in vivo due to inhibition of nitric oxide
synthase (iNOS) protein expression. R. graveolens, a related plant has been found
to reduce LPS-induced nitric oxide level (measured nitrite level) without altering
the cytokines (Iauk et al. 2004). The study of Raghav et al. (2006) is designed to
investigate the effect of plant extract of R. graveolens on murine macrophage cells
(J-774) challenged with lipopolysaccharide (LPS). The inhibition observed for the
extract was significantly higher than that observed for rutin, a flavonoid constituent
of the plant. At 40 μM rutin, a comparable concentration of this flavonoid in the
highest concentration (500 μg/ml) of plant extract was used in this study; a 20%
inhibition (p = 0.058) was observed. Inhibition in inducible nitric oxide synthase
(inos) gene expression in the cells treated with the plant extract suggests an inhibi-
tion at the transcription level.
R. graveolens extract exhibited a high inhibition on aldehyde oxidase activity
(89–96%) at 100 mg/ml which was comparable with 10 mM of menadione, a spe-
cific potent inhibitor of aldehyde oxidase (Saieed et al. 2006).
Extracts of rue have been proposed as topical pharmaceutical fungicides (Ali-
Shtayeh and Abu Ghdeib 1999; Trovato et al. 2000). Antifungal compounds were
found in callus cultures of R. graveolens (Wolters and Eilert 1981). Rue extracts and
two constituents of rue, 5- and 8-methoxypsoralen, are active against fungal plant
pathogens (Rhizoctonia solanii, Fusarium spp., Pyrenochaeta lycopersici,
Trichoderma viride, Penicillium spp., Thielaviopsis basicola, and Verticillium dahl-
iae) (Aliotta et al. 2000; Ojala et al. 2000; Oliva et al. 1999).
The constituents of R. graveolens were further studied not only because of inter-
est in the chemistry of natural products, but also because of several biologically
active compounds providing a base for the use of R. graveolens in folk medicine and
to find some more biologically active compounds. Bioassay-directed isolation of
antifungal compounds from an ethyl acetate extract of R. graveolens leaves yielded
two furanocoumarins, one quinoline alkaloid, and four quinolone alkaloids, includ-
ing a novel compound, 1-methyl-2-(6′-(3′′,4′′-methylenedioxyphenyl)hexyl)-
4-quinolone. Four of the alkaloids had moderate activity against Colletotrichum
species, including a benomyl-resistant Colletotrichumacutatum. These compounds
and the furanocoumarins 5- and 8-methoxypsoralen had moderate activity against
Fusarium oxysporum. The novel quinolone alkaloid was highly active against
Botrytis cinerea. Phomopsis species were much more sensitive to most of the com-
pounds, with P. viticola being highly sensitive to all of the compounds (Oliva et al.
2003).
The use of R. graveolens against intracranial cysticercosis has been reported
(Banerji and Banerji 2001). Recently Pathak et al. (2003) showed that homeopathi-
cally potentiated Ruta induces cell death in brain cancer cells. Telomere dynamics,
mitotic catastrophe and apoptosis have been shown to be the possible mechanisms.
An extract of R. graveolens was found to be cytotoxic to Dalton’s lymphoma ascites
(DLA), Ehrlich ascites carcinoma (EAC) and L929 cells in culture (IC100 = 16mg/
ml) and also to increase the lifespan of tumour bearing animals. The extract further
decreased solid tumours developing from DLA and EAC cells when given simulta-
neously with elongation of the lifespan of tumour-bearing animals. A homeopathic
preparation of R. graveolens (200c) was equally effective. Neither was effective for
reducing already developed tumours. The R. graveolensextract was found to scav-

enge hydroxyl radicals and inhibit lipid peroxidation at low concentrations.
However, at higher concentrations the extract acted as a prooxidant as inhibition of
lipid peroxidation and scavenging of hydroxyl radical was minimal. These data
indicate that the prooxidant activity of R. graveolens may be responsible for the
cytocidal action of the extract and its ability to produce tumour reduction (Preethi
et al. 2006).
The effects on the reproductive system and fertility using adult male albino rats
with special emphasis on the aggressive behavior and sex behavior was recently
study. The aqueous extract of R. graveolens solution was fed orally to male albino
rats at a dose of 500 mg/kg body weight for 60 days. This dose induces a significant
decrease in the weight of reproductive organs (P < 0.01) when compared to controls.
The sperm motility and density in cauda epidydimides and testicular ducts were
significantly decreased (P < 0.01). A significant decreased (P < 0.001) in spermato-
genesis activity is observed in somniferous tubule. Treated rats testicular cell popu-
lation showed a decrease in number of spermatocytes and spermatids (P < 0.001)
when compared to controls. Serum hormonal assay indicated a decrease in
Testosterone and Follicular Stimulating Hormone levels in treated rats. A decrease
in the number female rats impregnated by males receiving treatment was observed
and demonstrated by a decrease inthe implantation sites and viable fetuses number
(P < 0.01). The ingested extract also suppresses the sexual behavior in adult male
rats expressed by a prolongation of first mount time, increase in intromission
latency, decrease in intromissions number, and prolongation of the post-ejaculatory
interval. This led to reduce the ejaculation time and increase the post ejaculatory
intervals. Ingestion of R. graveolens markedly abolished aggressive behavior
parameters in adult male treated rats namely, a suppression in lateralization, boxing
bouts and ventral presenting postures (Khouri and EL-Akawi 2005).
Nineteen compounds have been isolated from the methanol extract of the root
and aerial parts of R. graveolens by (Wu et al. 2003). Their antiplatelet aggregation
andcytotoxic activities were examined to find potent antiplatelet aggregation and
cytotoxic compounds from natural resources. Among them, dictamine, skimmi-
anine, psoralen, chalepensin, clausindinand graveolinine showed significant inhibi-
tion of platelet aggregation, induced by arachidonic acid and collagen. Arborinine,
dictamine, isopimpinellin, clausindin, and graveoline exhibited cytotoxic activity
against KB, Hela, DLD, NCI and Hepa tumor cell lines (Wu et al. 2003).
The essential oil of aerial parts of R. graveolens was obtained by hydrodistilla-
tion with a 0.74% yield on a dry weight basis. Thirty-eight components were identi-
fied by GC and GC--MS analyses. 2-Ketones were predominant in the essential oil,
with undecan-2-one (46.8%) and nonan-2-one (18.8%) as the main constituents (De
Feo et al. 2002). Subcritical (CO2) extraction, carried out in a J&W Scientific High
Pressure Soxhlet Extractor, was used to isolate secondary metabolites from leaves,
flowers, stems and roots of Colombian rue (R. graveolens). The various extracts
were analyzed by capillary chromatography, on an HP-5 (30 m) column, using
nitrogen-phosphorus, flame ionization, and mass selective detection systems. The
extracts from the various parts of rue had different compositions. The number of
compounds detected at concentrations above 0.01% (w/w) in the extracts from

leaves, flowers, stems and roots, was 78, 45, 25 and 24, respectively. 2-Nonanone
(8.9%), 2-undecanone (13.4%), chalepensin (13.0%), and geijerene (19.3%) were
the main constituents found in the extracts from rue leaves, flowers, stems and roots,
respectively. Furanocoumarins, furoquinolines, hydrocarbons and benzodioxol
derivatives were the main compound families found in all extracts, at total concen-
trations between 3.7 and 33.9%, depending on the part of the plant (Stashenko et al.
2000).
Thymelaeaceae
Thymelaea hirsuta (L.) Endel.

Thymelaea is a genus comprising about 30 species of evergreen shrubs of the flow-
ering plant family Thymelaeaceae, native to the Canary Island, the Mediterranean
region, north of central Europe, and east of central Asia. In this report, we focused
on T. hirsuta, which is native to North Africa.
Thymelaea hirsuta (L.) Endel. is comonly known as “Methnane”. In Tunisia, this
plant has been used traditionally as an antiseptic and anti-inflammatory agent and
for the treatment of hypertension by external application. Murine melanoma B16
cells were treated with T. hirsuta extract, and then cell viability and synthesized
melanin content were measured. We found that the T. hirsuta extract decreased the
synthesized melanin content in B16 cells without cytotoxicity. Tyrosinase is a key
enzyme of melanogenesis and extracellular signal-regulated kinase (ERK)-1/2
phosphorylation is known to be related to melanogenesis inhibition. To clarify its
mechanism, authors also determined ERK1/2 phosphorylation and tyrosinase
expression level. ERK1/2 was immediately phosphorylated in cells just after treat-
ment with the extract. The tyrosinase expression was inhibited after 24 h of stimula-
tion with the extract. The same authors added that the fraction contains daphnanes
as the main component (Kawano et al. 2007).
A study on the effect of oral administration of the T. hirsuta aqueous extract on
blood glucose levels in normal, glucose-hyperglycemic and streptozotocin (STZ)-
induced diabetic rats showed that in normal rats, single oral administration of T.
hirsuta lowered blood glucose levels significantly. It omitted significantly reduced
the fasting glucose level in rats with oral glucose (2 g/kg) induced hyperglycemia.
In STZ-induced diabetic rats, single oral administration of T. hirsuta also produced
a significant decrease of blood glucose levels. The aqueous extract of T. hirsuta pos-
sesses both hypoglycaemic and antidiabetic effects in normoglycaemic and
streptozotocin-induced diabetic rats. This may indicate the ethnopharmacological
reason of the use of T. hirsuta in traditional medicine for treating diabetes (El-Amran
et al. El Amrani et al. 2009). The bioassay-guided fractionation of this plant implied
that the major bioactive components are daphnane diterpenoids. It was previously
reported that five daphnane diterpenoids were isolated from T. hirsuta (Brooks et al.
1990). These compounds are known to have various bioactivities, such as antileuke-
mic and neurotrophic effects (Park et al. 2007). The structures of two new daphnane
diterpenoids, hirseins A and B, from T. hirsuta, and their antimelanogenesis activi-
ties were reported.
Apiaceae (Umbelliferae)
Critimum maritmum L. called sea fennel, crest marine, marine fennerl, sampler
and also rock samphier, is a fleshy aromatic, perennial plant which grows wild on
maritime rocks, piers, and breakwaters, more rarely on sandy beaches along the
Mediterranean countries, pacific and Atlantic coasts. It grows on all the world’s
coastlines but is especially abundant in France, Italy, Portugal, Spain and Turkey
coastal regions (Barroso et al. 1991, 1992; Coiffard et al. 1993; Cunsolo et al. 1993).
C. maritimum is a facultative halophyte growing on maritime cliffs and sometimes
in sand. Several uses of this plant are known for culinary purposes. Indeed, C. mari-
timum leaves are rich in several compounds such as vitamin C, carotenoids, flavo-
noids, as well as bioactive substances which could be used for aromatic and
medicinal purposes (Ozcan 2000; Ruberto and Baratta 2000; Kumarasamy et al.
2002). Sea fennel contains fragrant oil other aromatic substances that are widely
used in cosmetics due to claimed slimming properties and medicine (Barroso et al.
1991; Senatore and De Feo 1994).
Its fresh leaves and young branches are pickled in vinegar and used as appetizers
and condiments (Ozcan 2000; Akgül 1993). The leaves are also used in flok medi-
cine as antiscorbutic, tonic, carminative, diuretic, depurative and vermifuge (Barroso
et al. 1991; Senatore and De Feo 1994; Ruberto et al. 1991).
Same results indicate that sea fennel can be considered as a valuable source of
antioxidant products, especially of chlorogenic acid (Meot-Duros and Magné 2009).
According to Guenther (1950), early studies on C. maritmum oil showed that all
parts of the plant contain volatiles compounds, but the yield and the chemical com-
position of the oils varied considerably depending on the site of growth and soil
conditions. The essential oil of C. maritmum showed major fluctuations in the rela-
tive amounts of several components, mainly sabinene (7–42%) and gamma terpi-
nene (26–55%) (Barroso et al. 1992). More recently, Ozcan et al. (2006) showed
that the essential oil obtained from young leaves and branches of C. maritmum
collected from Turkey contained mainly monoterpens, amounting to 89 % and 99,
respectively of the oil, with γ-terpinene (36% and 32%), β-phellandrene (21% and
22%), and sabinene (13% and 9%) as the main components.
Thapsia garganica L.
The genus Thapsia family Apiaceae (Umbelliferae) is widely distributed through-
out the Mediterraean area. Umbelliferous plants are popular as drugs and spices as
they contain useful secondary compounds such as essential oils, coumarins and
sequiterpenes (Hetwood 1971). The resin of Thapsia garganica causes contact
dermatitis and is extensively used by the Northern African Arabs for medicinal pur-
poses (Jager et al. 1993).
The major bioactive compounds synthesized by T. garanica are Thapsigargin,
Thapsigargicin, notrilobolid and thapsivillosin (Rasmussen et al. 1981). T. gargan-
ica, especially the root, contains potent skin irritants (Rasmussen et al. 1978).
Because of this property, described by Hippokrates about 400 B.C., drugs prepared
from the plant have been recorded in several pharmacopoieas, most recently the
1937 edition of the French pharmacopoiea. Two very potent skin irritants, named
thapsigargin (yield 0.1% of fresh material) and thapsigargicin (yield 0.02%), have
been isolated from an ethanolic extract of the root. The drugs are still used as ingre-
dients of rheumatic pain releasing ointments in Arabian folk medicine (Rasmussen
1978). The most famous compounds from either biosynthetical or pharmacological
point of view, have been isolated from T. garganica, which has become an indis-
pensable “tool” for the study of the sarco/endoplasmic calcium pump (SERCA)
(Olesen et al. 2004) and might offer a new type of chemotherapeutics for treatment
of prostate cancer (Denmeade and Isaacs 2005; Denmeade et al. 2003).
Pituranthos chloranthus L.
Pituranthos is the largest genus of the Apiaceae family and includes about 2500
species, which are spread throughout North Africa (Touil et al. 2006).
In Tunisia three species were mentioned by Pottier-Alapetite (1979), P. chloran-
thus; P. scoparius (Coss. et Dur.) and P. tortuosus (Coss. and Dur.). Furher,
Pituranthos species are used in traditional medicine for the treatment of asthma,
rheumatism, postpartum care, spasms, pains, fevers, diabetes, lice (head and pubis),
hepatitis, digestives difficulties, urinary infections and scorpions stings (Verite et al.
2004; Yangui et al. 2009). Stems of P. chloranthus have been, traditionally, used as
straw for farmers to dry figs and grapes. This plant has a double advantage; first, it
is used for its aroma and distinctive taste that adhere to the dry fruits. Second, it has
an insecticidal effect. In the South of Tunisian, a tuft of P. chloranthus was tradition-
ally suspended on the surface of drinking water to disinfect the underground cis-
terns of the rainwater storage.
As a consequence, some species of Pituranthos, for example P. tortuosus, have
been investigated for biological activity (Abdelwahed et al. 2006). Pituranthos
essential oil and its main constituents are considered as a potential source of bio-
logically active compounds (Le Floc’h 1983). The P. chloranthus essentials oils
reduced significantly Benzo (a) pyrene (B(a) P) and sodium-azide-induced muta-
genicity. The antioxidant activity was also showed. More recently Neffati et al.
(2009) showed that essential oils of P. chloranthus could notably protect against
damage induced by radicals. Dahia et al. (2007) identified 54 volatile constituents
in the essential oil of P. chloranthus harvested in Algeria at the flowering stage. One
hundred and fifty compounds were identified in which α-pinene, β-pinene,
α-phellandrene, β-myrcene, β-phellandrene, p-cymene, 8-methyldecanal, exo-2-
hydroxycineole acetate and carvacrol could reach more than 10 % of the total
amount of Tunisian P. chloranthus (Neffati et al. 2009).
Pituranthos tortuosus (Coss.) Maire

P. tortuosus, known in Arabic as “Guezzah”, is a small shrub without leaves. This
aromatic plant, which grows naturally in North Africa, is widespread in central and
southern Tunisia (Pottier-Alapetite 1981). In Tunisia, P. tortuosus is used tradition-
ally as an anti-asthmatic and against scorpion stings (Boukef et al. 1982). This spe-
cies is used by the Egyptians for the preparation of a carminative drink and is
occasionally eaten by grazing animals (Ahmed et al. 1962). It is also used for relief
of stomach pains, against intestinal parasites, when blood is excreted in the urine or
when coughing blood, and for the regulation of menstruation (Ashkenazy et al.
1984).
Plants of the family Apiaceae are generally known to be rich in essential oils
showing antimicrobial activity against bacteria and fungi (Al-Gaby and Allam
2000). The essential oil of P. tortuosus from Egypt is a mixture of a number of vola-
tile components, mainly terpenoids, with camphene (31%) as the major constituent,
of the oil. This oil was ineffective against Gram-positive bacteria but exhibited anti-
fungal activity at high concentrations (Al-Gaby and Allam 2000). The aerial parts of
P. tortuosus, collected during November and April from Tunisia, were analysed by
GC and GC–MS. In total, 56 compounds were identified by their retention indices.
Antimicrobial assays showed that the essential oil obtained from plants in November
is more effective against the Gram-positive bacteriathan the oil of April. Enterococcus
faecalis and Staphylococcus aureus. The essential oil obtained in April displayed
the highest activity against Staphylococcus aureus (Abdelwahed et al. 2006).
Mutagenic and antimutagenic activities against direct acting mutagens, nifuroxa-
zide (NF) and sodium azide (SA), and indirect acting mutagen aflatoxin B1 (AFB1)
of extracts prepared from aerial parts of P. tortuosuswere investigated in bacterial
assay systems (i.e., the Ames test with Salmonella typhimurium TA100, TA98,
TA1538, TA1535, and the SOS chromotest with Escherichia coli PQ 37). It was
found that all extracts obtained from P. tortuosus decreased the mutagenicity
induced by AFB1 (10 μg/assay), SA (1.5 μg/assay), and NF (20 μg/assay). Ethyl
acetate, acetone, methanol, and total oligomer flavenoid extracts exhibited the high-
est inhibition level of mutagenicity induced by the indirect mutagen AFB1
(Abdelwahed et al. 2008). In addition, antiproliferative and apoptotic properties of
these extracts have also been reported using two leukemia cell lines, L1210 and
K562. The results revealed that all extracts showed a significant cytotoxic effect on
these cell lines, and the effect was greater in the presence of human K562 chronic
myelogenous leukemia cells, whereas they do not induce apoptosis (Abdelwahed
et al. 2008).
Using different spectroscopic techniques, the isolated compounds from the
CHCl3 soluble fraction of the roots of P. tortuosus were identified as bergapten,
graveolone, xanthotoxin, isopimpinellin, aesculetin dimethyl ether, stigmasterol
glucoside, in addition to the new ester 4-methoxyphenylumbellate (Abdel-Kader
2003).
Zygophyllaceae
Nitraria retusa (Forsk) Asch. is native to the desert areas of Northern Africa. N.
retusa (Forssk.) Asch is one of the leading shrubs in steppes, deserts and saline soils.
It belongs to the family Nitrariaceae where it is the only genus of this family
(Täckholm 1974; Boulos 2000) or to the family Zygophyllaceae (Zohary 1972). It
has a fairly continuous range of distribution extending from Arabia in the north to
Somalia in the south, and from Palestine in the east to Senegambia in the west
(Zohary 1973). It is definitely a Saharo-Arabian species with some trends towards
Sudanian territories. It has tiny, white to green, fragrant flowers and small edible red
fruits. Along with its culinary use, N. retusa is also used in folk medicine. N. retusa
aqueous extracts showed significant anti-inflammatory activities.
Peganum harmala (L.)
Peganum harmala L. is a perennial herbaceous, glabrous plant, which may grow up
to 30–100 cm. Its normal habitat is semi-arid rangeland, steppe areas and sandy
soils. The plant is widely distributed in the Central Asia, North Africa and Middle
East and has been introduced in America and Australia. This plant is known as
“Espand” in Iran, “Harmel” in North Africa and “African Rue”, “Mexican Rue” or
“Turkish Rue” in United States. It has alternate thong-like leaves, which have a
strong deterrent odor when rumpled. Opposite to the leaves are solitary white flow-
ers with green veins (Mahmoudian et al. 2002).
Seeds of P. harmala, a widespread species growing wild in Turkey are used as an
antiheamoroidal, helmicide, and central nervous system (CNS) stimulating agent in
folk medicine (Baytop 1999 and Kusmenoglu 1996). P. harmala has been used in
Turkey for ritual purification for drift away the evil spirits by burning the seeds. It is
used for dyes in Turkish rugs, it was source of the richly coloured textile dye (Baytop
1999). In traditional medicine in the Middle East and North Africa, this plant is used
as an anthelmintic, lactogogue, antispasmodic, antipyretic, abortifacient, emetic and
emmenagogue (Chopra et al. 1958; Nadkarni 1954). Its ethnobotanical preparation
is used in the treatment of cancer by the late Hakim Analoui, a local healer in Iran,
is a mixture of the extracts of P. harmala L. (Zygophyllaceae) and Dracocephalum
kotshyi Boiss. (Labiatae). Eighty percent of this local remedy is P. harmala seeds
extract. Its cytotoxic effects on cancerous cell-lines have been demonstrated in pre-
vious studies (Lamchouri et al. 1999; Lamchouri et al. 2000; Sobhani et al. 2002).
P. harmala alkaloids (Munir et al. 1993) are known for their antimicrobial
(Ahmed et al. 1992), hypothermic (Abdelfattah et al. 1995) and hallucinogenic
(Bruinvels 1969; Stratton and Lorden 1991; O’Hearn and Molliver 1993) proper-
ties. In Moroccan traditional medicine, seeds of P.harmala have been used for the
empiric treatment of cancers (Bellakhdar 1997). The alkaloidic fraction of the meth-
anol extract of P. harmala seeds was tested in vitro on three tumoral cell-lines:
UCP-Med and Med-mek Carcinoma, and UCP-Med Sarcoma. Proliferation was
significantly reduced at all tested concentrations (20–120 μg/ml) during the first 24
h of contact. A cell lysis effect occurred after 24 h and increased thereafter to com-
plete cell death within 48–72 h, depending on tested concentration (Lamchouri
et al. 2000). P. harmala is a poisonous plant that grows in Central Asia, North Africa
and Middle East
The pharmacologically active compounds of P. harmala are several alkaloids,
which are contained found especially by the seeds and the roots. These include
β-carbolines such as: harmine, harmaline (identical with harmidine), harmalol and
harman and quinazoline derivatives: vasicine and vasicinone. The alka-loidal con-
tent of the unripe seeds is less than the ripeones (Kamel et al. 1970). Harmaline
(harmidine, C13H15ON2), first isolated by Göbel (Budavari and O’Neil 1996) from
the seeds and roots of P. harmala, this is the major alkaloid of this plant. Harmaline
is almost twice as toxic as harmine and in moderate doses causes tremors and clonic
convulsions but with no increase in spinal reflex excitability (Glasby 1978). Lethal
doses bring about convulsions, which are soon followed by motor paralysis due to
the marked depressive action upon the central nervous system. Respiration is para-
lyzed and a decrease in body temperature occurs. The perfused heart is arrested in
diastolic phase and the con-tractions of smooth muscle are diminished with the ex-
ception of the uterus, which may be made to contract more powerfully. Over a wide
range of doses there is a reduction in blood pressure due to a pronounced weakening
of the heart muscle.
Harmine (banisterine, C13H12ON2) is present in P. harmala. Pharmacologically,
harmine resembles harmaline in its actions but is less toxic. The hydrochlo-ride has
been found to be highly active against Myco-bacterium tuberculosis (Glasby 1978).
Harmalol (C12H12ON2) which occurs in P. harmala crystallizes from water as the
trihydrate. It is freely soluble in hot water, acetone or chloroform but only sparingly
soluble in benzene. The alkaloid is unstable when exposed to air. Its methyl ether is
harmaline (Glasby 1978).
Vasicine (peganine, C13H15ON2), discovered in P. harmala under the name of
Peganine. The salt are readily obtained as crystals and is used in India as a remedy
for asthma and the pure alkaloid acts as a bronchodilator (Puzii et al. 1980).
Vasicinone (C11H10O2N2) a further alkaloid present in P. harmala. The base
forms colorless crystals from 95% alcohol. It has (α) 22–100° (c = 0.5 in CHCl3)
and the UV spectrum has absorption maxima at 227, 272, 302 and 315 nm. The
alkaloid yields crystalline salts with mineral acids. It is an active bronchodilator
(Puzii et al. 1980). In addition to their effects on CNS (Airaksinen and Kari 1981)
and cardiovascular system (Aarons et al. 1977), there are reports of antimicrobial
(Ahmed et al. 1992) and cytotoxic activities (Sobhani et al. 2002; Ahmed et al.
1992) of β-carbolines.
Zygophyllum album L.
Zygophyllum album L. is a succulent plant belonging to the Zygophyllaceae family
that adapts to dry environments such as rock walls, the warm and temperate regions.
Folk medicine uses a variety of species of Zygophyllum, including Z. simplex L., Z.
gaetulum, Z. cornutum Coss. and Z. album L. for therapeutic treatments. It was
reported that different parts of Zygophyllum are used against diabetes, and as a drug
active against rheumatism, gout, asthma and hypertension. It is also used as diuretic,
local anaesthetic, antihistaminic, anti-inflammatofry, molluscicidal and expectorant
activity (Pöllmann et al. 1997; Safir and Fkih-Tetouani 1998; Jaouhari et al. 1999;
Jaouhari and Lazrek 2000; Rimbau et al. 1999). Local surveys show that Z. album
L. is used against aches and for calm thirst. It is also used for wound care, treatment
of dental caries, and washing clothes and hair (Grim and Bounaga 1996).
The essential oil of Z. album was extracted, by hydrodistillation (HD) and analy-
sis performed by gas chromatography and gas chromatography coupled to mass
spectrometry which showed that (E)-β-damascenone was the major component of
the oil (Tigrine-Kordjani et al. 2006a). Employing microwave accelerated distilla-
tion (MAD) extraction (100 °C, 30 min), Z. album essential oil contained mainly
oxygenated monoterpenes with major constituents: carvone and a-terpineol.
Remarkably, most of the compounds present in the hydrodistilled volatile fraction
were not terpene species, with β-damascenone as a major constituent (Tigrine-
Kordjani et al. 2010). Further investigations into the leaves and stems of Z. album
yielded four triterpene glycosides which were characterized as 14-decarboxyquinovic
acid-3β-O-β-d-quinovopyranosyl (1→4)-quinovopyranoside, quinovic acid 28-O-β-
d-glucopyranosyl (2→1)β-d-glucopyranosyl ester, quinovic acid 27-β-d-glucopy
ranosyl(2ster-d-glucopyranosyl ester and quinovic acid-3-β-O-glucopyranosyl(2ster
and quinovic acid-3-β-uinovic acid 28-le fraction were not terpenes (Hassanean
et al. 1993). Besides the two known saponins, quinovic acid-3β-O- β -D-quinovoside
and 3β-O- β -D quinovopyranosyl quinovic acid (28→l) β-D-glycopyranosyl ester,
two new glycosides having the structure quiaovic β acid 3β-O- β -D quinovopyrano-
syl(3→1) β-D-xylopyranoside and 3β-O- β -D quinovopyranosylquinovic acid
(28→ l)quinovopyra-nosy1 ester were isolated from the aerial parts of Z. album
growing in Egypt (Hassanean et al. 1993).
References
Aarons DH, Rossi GV, Orzechowski RF (1977) Cardiovascular actions of three harmala alkaloids:
harmine, harmaline, and harmalol. J Pharm Sci 66:1244–1248
Abdallah L, Chaieb M (2007) Water status and growing phonology of a Saharan Shrub in North
Africa. Afr J Ecol 1(45):80–85
Abdalla MF, Saleh NAM (1983) Flavonoids of Retama raetam. J Nat Prod 46:755–756.
Abdel Halim OB, Abdel Fattah H, Halim AF, Murakoshi I (1997) Comparative chemical and bio-
logical studies of the alkaloidal content of Lygos species and varieties growing in Egypt. Acta
Pharm Hung 67:241–247
Abdel-Kader MS (2003) New ester and furanocoumarins from the roots of Pituranthos tortuosus.
J Braz Chem Soc 14:48–51
Abdel-Fattah AFM, Matsumoto K, Gammaz HAK, Watanabe H (1995) Hypothermic effect of
harmala alkaloid in rats: involvement of serotonergic mechanism. Pharmacol Biochem Behav
52:421–426
Abdelwahed MSA, Amin AA, El-Rashad SM (2006) Physiological effect of some bioregulators
on vegetative growth, yield and chemical constituents of yellow maize plants. World J Agric
Sci 2:149–155
Abdelwahed A, Skandrani I, Kilani S, Neffati A, Sghaier MB, Boubaker J, Ammar RB, Mahmoud
A, Ghedira K, Chekir-Ghedira L (2008) Mutagenic, antimutagenic, cytotoxic and apoptotic
activities of extracts from Pituranthos tortuosus. Drug Chem Toxicol 31(1):37–60
Abdel-Zaher AO, Salim SY, Assaf MH, Abdel-Hady RH (2005) Antidiabetic activity and toxicity
of Zizyphus spina-christi leaves. J Ethnopharmacol 101:129–138
Abdollahi M, Fooladian F, Emami B, Zafari K, Bahreini-Moghadam A (2003) Protection by silde-
nafil and theophylline of lead acetate-induced oxidative stress in rat submandibular gland and
saliva. Hum Exp Toxicol 22:587–592
Adam SEI (1978) C/in. 7bxicol 13:269
Adams RP (1999) Systematics of multi-seeded eastern hemisphere Juniperus based on leaf essen-
tial oils and RAPD DNA fingerprinting. Biochem Syst Ecol 27:709–725
Adams RP (2000) Systematics of Juniperus section Juniperus based on leaf essential oils and ran-
dom amplified polymorphic DNAs (RAPDs). Biochem Syst Ecol 28:515–528
Adams RP, Barrero AF, Lara A (1996) Comparisons of the leaf essential oils of Juniperus phoeni-
cea, J. phoenicea subsp. eu-mediterranea Lebr. & Thiv. and J. phoenicea var. turbinata (Guss)
Parl. J Essent Oil Res 8:367–371
Adhvaryu MR, Reddy N, Parabia MH (2007) Effects of four Indian medicinal herbs on Isoniazid-,
Rifampicin- and Pyrazinamide-induced hepatic injury and immunosuppression in guinea pigs.
World J Gastroenterol 13:3199–3205
Adzu B, Amos S, Amizan MB, Gamaniel K (2003) Evaluation of the antidiarrhoeal effects of
Zizyphus spina-christi stem bark in rats. Acta Trop 87(2):245–250
Afsharypuor S, Jeiran K, Jazy AA (1998) First investigation of the flavor profiles of leaf, ripe fruit
and root of Capparis spinosa var. mucronifolia from Iran. Pharm Acta Helv 72:307–309
Ahmed A, Cameron IT, Ferriani RA (1992) Activation of phospholipase A2 and phospholipase C
by endothelin-1 in human endometrium. J Endocrinol 135:383–390
Ahmed MS, Galal AM, Ross SA, Ferreira D, El Sohly MA, Ibrahim ARS, Mossa JS, El-Ferly FS
(2001) A weakly antimalarial biflavonone from Rhus retinorrhoea. Phtochemistry 58:599–602
Ahmed ZF, Wassel GM, Abdel-Moneim FM (1969) A preliminary phytochemical investigation of
pituranthostortuosus (Desf.) Benth and Hook. J Pharm Sci U A R 10(1):31–36
Airaksinen MM, Kari I (1981) b-Carbolines, psychoactive compounds in the mammalian body.
Effects Med Biol 59:190–211
Akbay P, Gertsch J, Çalis I, Heilmann J, Zerbe O, Sticher O (2002) Novel antileukemic sterol
glycosides from Ajuga salicifolia. Helv Chim Acta 85:1930–1942
Akgül A (1993) Spice science and technology (Baharat Bilimi ve Teknolojisi; in Turkish) Turkish.
Association of Food Technologists, Publ. No. 15, Ankara
Akrout A (2004) The study of chemical compositions of essential oils of three pastoral plants from
Matmata (south Tunisia) (in French). Cah Options Méditerr 62:289–292
Akrout A, Chemli R, Chrief I, Hammami M (2001) Analysis of the essential oil of Artemisia camp-
estris L. J Flavour Fragr 16:337–339
Akrout A, Chemli R, Simmonds M, Kite G, Hammami M, Cherif I (2003) Seasonal variation of the
essential oil of Artemisia campestris L. J Essent Oil Res 15(5):333–336
Akrout A, El-Jani H, Amouri A, Neffati M (2010a) Screening of antiradical and antibacterial activ-
ities of essential oils of Artemisia campestris L., Artemisia herba-alb Asso. & Thymus capita-
tus Off. et Link. Growing wild in the southern of Tunisia. Recent Res Sci Technol 2(1):29–39
Akrout A, Hajlaoui H, Mighri H, Najjaa H, El Jani H, Zaidi S, Neffati M (2010b) Chemical and
Biological Characteristics of Essential Oil of Rosmarinus officinalis Cultivated in Djerba.
Jeobp 13(4):398–411
Alçiçek Z (2011) The effects of thyme (Thymus vulgaris L.) oil concentration on liquidsmoked
vacuum-packed rainbow trout (Oncorhynchus mykiss Walbaum, 1792) fillets during chilled
storage. Food Chem 128:683–688
Al-Gaby AM, Allam RR (2000) Chemical analysis, antimicrobial activity and the essential oils
from some wild herbs in Egypt. J Herbs Species Med Plant 7(1):15–23
Alghazeer R, El-Saltani H, Saleh N, Al-Najjar A, Hebail F (2012) Antioxidant and antimicrobial
properties of five medicinal Libyan plants extracts. Nat Sci 4(5):324–335
Aliotta G, Pollio A (1994) Useful plants in renal therapy according to Pliny the elder. Am J Nephrol
14:341–399
Aliotta G, Caiero G, De Feo V, Di Blasio B, Iacovino R, Oliva A (2000) Allelochemicals from rue
(Ruta graveolens L.) and olive (Olea europaea L.) oil mill waste waters as potential natural
pesticides. Curr Top Phytochem 3:167–177
Al-Mustafa AH, Al-Thunibat OY (2008) Antioxidant activity of some Jordanian Medicinal plants
used traditionally for treatment of diabetes, Pakistan. Aust J Biol Sci 1(3):351–358
Al-Okbi SY, El-Sayed EM, Ammar NM, El-Sayed NK, Abou-El Kassem LT (2002) Effect of Ruta
graveolens L. and Euphorbia peplus L. anti-inflammatory extracts on nutritional status of rats
and the safety of their use. Indian J Exp Biol 40(1):45–48
Al-Quran S (2008) Taxonomical and pharmacological survey of therapeutic plants in Jordan. J Nat
Prod 1:10–26
Al-Rawi A, Chakravarty HL (1964) Medicinal plants of Iraq. Ministry of Agriculture Technical
Bulletin No. 15, Govt. Press, Baghdad.
Al-Said MS, Abdelsatter EA, Khalifa SI, El-Ferly FS (1988) Isolation and identification of an anti-
inflammatory principle from Capparis spinosa. Pharmazie 43:640–641
Al-Sereitia MR, Abu-Amerb KM, Sena P (1999) Pharmacology of rosemary (Rosmarinus officina-
lis Linn.) and its therapeutic potentials. Indian J Exp Biol 37:124–131
Ali-Shtayeh MS, Abu Ghdeib SI (1999) Antifungal activity of plant extracts against dermato-
phytes. Mycoses 42:665–672
Amaral FMM, Ribeiro MNS, Barbosa-Filho JM, Reis AS, Nascimento FRF, Macedo RO
(2006) Plants and chemical constituents with giardicidal activity. Rev Bras Farmacogn
16(Supl.):696–720
Amer A, Mehlhorn H (2006a) Larvicidal effects of various essential oils against Aedes anopheles
and Culex larvae (Diptera, Culicidae). Parasitol Res 99:466–472
Amer A, Mehlhorn H (2006b) Repellency effects of forty-one essential oils against Aedes anoph-
eles and Culex mosquitoes. Parasitol Res 99:478–490
Amico FP, Sorce EG (1997) Medical plants and phytotherapy in Mussomeli area (Caltanisseta,
Sicily, Italy). Fitoterapia 68:143–159
Amirti F, Aberchane M (2008) Activité antifongique des huiles essentielles de Thymus bleicheria-
nus Pomel et Thymus capitatus (L.) Hoff. & Link contre les champignons de pourriture du bois
d’eouvre. BASE 12(4):345–351
Amirti F, Satrani B, Aafi A, Ghanmi M, Farah A, Aberchane M, El Ajjouri M, Al Antry S, Chaouch
A (2008a) Chemical composition and antimicrobial activity of the essential oils of Moroccan
Thymus capitatus and Thymus bleicherianus. Phytothérapie 6(6):342–347
Angelini G, Vena GA, Filotico R, Foti C, Grandolfo M (1991) Allergic contact dermatitis from
Capparis spinosa L. applied as wet compresses. Contact Dermatitis 24(5):382–383
Aniya Y, Shimabukuro M, Shimoji M, Kohatsu M, Gyamfi MA, Miyagi C, Kunii D, Takayama F,
Egashira T (2000) Antioxidant and hepatoprotective actions of the medicinal herb Artemisia
campestris from the Okinawa Islands. J Biol Pharm Bull 23(3):309–312
Anonymous (2000) Studies on the cytotoxicity and antimicrobial effects of the extract of Ajuga
multiflora Bunge. Korean J Pharmacogn 31:72–76
Arena A, Bisignano G, Pavone B, Tomaino A, Bonina FP, Saija A (2008) Antiviral and immuno
modulatory effect of a lyophilized extract of Capparis spinosa L. buds. Phytother Res
22:313–317
Arnault I, Haffner T, Siess MH, Vollmard A, Kahane R, Auger J (2005) J Pharm Biomed Anal
37:963–970
Ashkenazy D, Friedman J, Kashman Y, Egyed MN, Shlosberg A, Nyska A (1984) Photosensitization
in ducklings induced by Pituranthos triradiatus. Vet Hum Toxicol 26(2):118–120
Askri M, Mighri Z, Bui AM, Das BC, Hylands PJ (1989) Medicinal plants of Tunisia. The struc-
ture of periplocadiol, a new elemane-type sesquiterpene isolated from the roots of Periploca
laevigata. J Nat Prod 52:792–796
Auger J, Yang W, Arnault I, Pannier F, Potin-Gautier M (2004) J Chromatogr A 1032:103–107
Avallone R, Plessi M, Baraldi M, Monzani A (1997) Determination of chemical composition
of carob (Ceratonia siliqua): protein, fat, carbohydrates and tannins. J Food Compos Anal
10:166–172
Ayaz FA, Torun H, Ayaz S, Correia PJ, Alaiz M, Sanz C, Gruz J, Strnad M (2007) Determination
of chemical composition of Anatolian carob pod (Ceratonia siliqua L.): sugars, amino and
organic acids, minerals and phenolic compounds. J Food Qual 30(6):1040–1055
Baba Aïssa F (1991) Les Plantes Me´dicinales en Algérie. Coédition Bouchène et Ad-Diwan, Alger
Bakkali F, Averbeck S, Averbeck D, Idaomar M (2008) Biological effects of essential oils – a
review. Food Chem Toxicol 46:446–475
Bakri LM, Douglas CWI (2005) Inhibitory effect of garlic extract on oral bacteria. Arch Oral Biol
50(7):645–651
Banarjee SP, Dandiya PC (1967) smooth muscle and cardiovascular pharmacology of α-elaterin-2-
D-glucopyranoside glycoside of Citrullus colocynthis. J Pharm Sci 56(12):1665–1667
Banerji P, Banerji P (2001) Intracranial cysticercosis: an effective treatment with alternative medi-
cines. In Vivo 15:181–184
Bannour F, Bel Haj Saleh K, Chraief I, Hammami M, El Gazzah M, Chemli R, Aouni M (2006)
Variability in essential oil composition and antibacterial activity of Tunisian Rosmarinus offici-
nalis. Microbiol Hyg Alim 18(51):59–64
Banthorpe DV, Davies HS, Gatford C, Williams SR (1973) Monoterpene patterns in Juniperus and
Thuja species. Planta Med 23:64–69
Baranauskiene R, Venskutonis PR, Viskelis P, Dambrauskiene E (2003) Influence of nitrogen
fertilizers on the yield and composition of thyme (Thymus vulgaris). J Agric Food Chem
51(26):7751–7758
Barroso JG, Pedro LG, Pais MSS, Scheffer JJC (1991) Analysis of the essential oil of Crithmum
maritimum L. J Essent Oil Res 3:313–316
Barroso JG, Pedro LG, Figueiredo AC, Salomé M, Pais S (1992) Seasonal variation in the compo-
sition of the essential oil of Crithmum maritimum L. Flavour Fragr J 7:147–150
Baytop T (1984) Therapy with medicinal plants (past and present). Istanbul University publica-
tions, Istanbul, pp 268–269
Baytop T (1999) Turkçe Bitkiler ile Tedavi-Geçmisten Bugüne (Therapy with Medicinal Plants in
Turkey-Past and Present), 2nd edn. Nobel Tip Basimevi, Istanbul, pp 348–349
Bedir E, Tasdemir D, Çalis I, Zerbe O, Sticher O (1999) Neo-clerodane diterpenoids from Teucrium
polium. Phytochemistry 51:921–925
Bekkara FA, Bousmaha L, Bendiab SAT, Boti JB, Casanova J (2007) Chemical composition of
essential oil cultivated and wild growing Rosmarinus officinalis L. from Tlemcen (Algeria).
Biology & Health 7(1):6–11. (in french)
Bellakhdar J (1996) La Pharmacopée Marocaine Traditionnelle. Ibiss Press, p 358
Bellakhder J (1997) La Pharmacopée Marocaine Traditionnelle. Ibis Press, Paris, pp 271–272
Bellakhdar J, Claisse R, Fleurentin J, Younos C (1991) J Ethnopharmacol 35:123–143
Bellei B, Maresca V, Flori E, Pitisci A, Larue L, Picardo M (2010) Regulates pigmentation via
proteasomal degradation of tyrosinase. J Biol Chem 285:7288–7299
Bello R, Moreno L, Primo-Yufera E, Esplugues J (2002) Phytother Res 16:389–392
Ben El Hadj Ali I, Zaouali Y, Bejaoui A, Boussaid M (2010) Variation of the chemical composition
of essential oils in Tunisian populations of Thymus algeriensis Boiss. et Reut (Lamiaceae) and
implication for conservation. Chem Biodivers 7:1276–1289
Ben Jannet H, Harzallah-Skhiri F, Mighri Z, Simmonds MSJ, Blaney WM (2001) Antifeedant
activity of plant extracts and of new natural diglyceride compounds isolated from Ajuga
pseudo-iva leaves against Spodoptera littoralis. Ind Crop Prod 14:213–222
Ben Jannet H, Harzallah-Skhiri F, Mighri Z, Simmonds MSJ, Blaney WM (2000) Responses of
Spodoptera littoralis larvae to Tunisian plant extracts and to neo-clerodane diterpenoids iso-
lated from Ajuga pseudoiva leaves. Fitoterapia 71:105–112
Ben Salah H, Bouaziz M, Bahroun A, Damak M, McKillop M, Simmonds MSJ (2000) Contribution
à l’étude chimique Hyparrhenia hirta. J Soc Chim Tunis 5(7):647–652
Ben Salah H, Jarraya R, Martin MT, Veitch NC, Grayer RJ, Simmons MSJ, Damak M (2002)
Flavonol triglycosides from the leaves of Hammada scoparia (POMEL) ILJIN. Chem Pharm
Bull 50(9):1268–1270
Benkrief R, Brum-Bousquet M, Tillequin F, Koch M (1990) Alkaloids and flavonoid from aerial
parts of Hammada articulata spp scoparia. Ann Pharm Fr 48:219–224
Bennaghmouch L, Hajjaji N, Zellou A, Cherrah Y (2001) Pharmacological study of Ajuga iva. Ann
Pharm Fr 59:284
Bhargava R, Shukla AK, Chauhan N, Vashishtha BB, Dhardar DG (2005) Impact of hybridity on
flavonoid spectrum of ber (Ziziphus mauritiana Lamk.) Environ Exp Bot 53:135–138
Binder RJ, Coit JE, Williams KT, Brekke JE (1959) Carob varieties and composition. Food
Technol 13:213–216
Biondi D, Cianci P, Geraci C (1993) Antimicrobial activity and chemical composition of essential
oils from Sicilian aromatic plants. J Flavour Fragr 8:331–337
Bloedorn WA, Dickens PF (1928) Danger of the administration of ephedrine in heart failure. Arch
Intern Med 42:322–330
Bondi ML, Al-Hillo MRY, Ladjel S, Bruno B, Piozzi F, Simmonds MSJ (2000) Occurrence of the
antifeedant 14,15-dihydroajugaritin in the aerial parts of Ajuga iva from Algeria. Biochem Syst
Ecol 28(10):1023–1025
Bonnier G, Douin R (1990) La grande flore. Belin, Paris
Borgi W, Bouraoui A, Chouchane N (2007) Antiulcerogenic activity of Zizyphus lotus (L.) extracts.
Borkowski B, Pasich B (1958) Die isolation der flavone aus herba Herniariae glabrae L. Planta
Med 6(3):225–237
Bouaziz M, Simmonds MSJ, Grayer RJ, Kite GC, Damak M (2001) Flavonoids from Hyparrhenia
hirta Stapf (Poaceae) growing in Tunisia. Biochem Syst Ecol 29:849–851
Bouaziz M, Veitch NC, Grayer RJ, Simmonds MSJ, Damak M (2002) Flavonolignans from
Hyparrhenia hirta. Phytochemistry 60:515–520
Bouchouka EL, Djilani A, Bekkouche A (2012) Antibacterial and antioxidant activities of three
endemic plants from Algerian Sahara. Acta Sci Pol Technol Aliment 11(1):61–65
Boukef MK (1986) Les Plantes dans la médicine traditionnelle Tunisienne, Agence de Coopération
Culturelle et Technique, pp 82–83
Boukef MK, Souissi HR, Ballansard G (1982) Contribution à l’étude des plantes utilisées en médi-
cine traditionnelle tunisienne. Plants Med Phyto 16:260–279
Boukrich F, Zouari S, Neffati M, Abdelly C, Liu K, Casanova J, Tomi F (2010) J Essent Oil Res
22:331
Boulos L (1983) Medicinal plants of North Africa. Reference Publications, Algonac
Boulos L (2000) Flora of Egypt. Vol. II (Geraniaceae- Boraginaceae). Al-Hadara Publishing,
Cairo, p 352
Boumaza O, Mekkiou R, Seghiri R, Sarri D, Benayache S, Garcia VP, Benayache F (2006)
Flavonoids and isoflavonoids from Genista tricuspidata. Chem Nat Compd 42(6):730–731
Bounatirou S, Smiti S, Miguel MG, Rejeb MN, Neffati M, Costa MM, Faleiro L, Figueiredo AC,
Barroso JG, Pedro LG (2007a) Chemical composition, antioxidant and antibacterial activi-
ties of the essential oils isolated from Tunisian Thymus capitatus Hoff. Et Link. Food Chem
105:146–155
Bounatirou SG, Zouari S, Figueiredo ACS, Barroso JG, Pedro LG, Neffati M, Rejeb MN, Smiti S
(2007b) Chemical homogeneity of Thymus capitatus Hoff. et Link. essential oils from Tunisia.
Revue des Régions Arides 2:679–686
Bounatirou S, Smiti S, Miguel MG, Rejeb MN, Neffati M, Costa MM, Faleiro L, Figueiredo
AC, Barroso JG, Pedro LG (2008) Tymus capitatus grown in Tunisia: antioxidant ability of
the essential oils on lilnoleic acid evaluated by different methods. Acta Horticulturae (ISHS)
765:315–324
Bourogaa E, Bertrand J, Despeaux M (2011) Hammada scoparia flavonoids and rutin kill adherent
and chemoresistant leukemic cells. Leuk Res 35:1093–1101
Bourogaa E, Nciri R, Sulta CR, Damak M, Elfeki A (2012) Antioxidant activity and hepatopro-
tective potential of Hammada scopariaagainst ethanol-induced liver injury in rats. J Physiol
Biochem
Boutekedjiret C, Bentahar F, Belabbes R, Bessière JM (1998) The essential oils of Rosmarinus

officinalis from Algeria. J Esent Oil Res 10:680–682
Boutekedjiret C, Belabbes R, Bentahar F, Bessière JM (1999) Study of Rosmarinus officinalis L.
essential oil as a function of the plant life cycle. J Esent Oil Res 11:238–240
Brandão MGL, Cosenza GP, Moreira RA, Monte-Mor RLM (2006) Medicinal plants and other
botanical products from the Brazilian Official Pharmacopoeia. Rev Bras Farm 16:408–420
Bravo L, Grados N, Saura-Calixto F (1994) Composition and potential uses of mesquite pods
(Prosopis pallida L.). Comparison with carob pods (Ceratonia siliqua L.) J Sci Food Agric
65:303–306
Bremner PD, Simmonds MSJ, Blaney WM, Veitch NC (1998) Neo-clerodane diterpenoid insect
antifeedants from Ajuga reptans ev Catlins Giant. Phytochemistry 47:1227–1232
Breschi MC, Martinotti E, Catalano S, Flamini G, Morelli I, Pagni AM (1992) Vasoconstrictor
activity of 8-Oacetylharpagide from Ajuga reptans. J Nat Prod 55:1145–1148
Brevard H, Brambilla M, Chaintreau A, Marion JP (1992) Occurrence of elemental sulphur
in capers (Capparis spinosa L.) and first investigation of the flavour profile. Flavour Fragr
J 7:313–321
Brigelius-Flohé R, Traber MG (1999) Vitamin E: function and metabolism. FASEB J 13:1145–1155
Brooks G, Evans AT, Aitken A, Evans FJ, Rizk AFM, Hammouda FM, El-Missiry MM, Ismail SE
(1990) Daphnane diterpenes of Thymelaea hirsuta. Phytochemistry 29(7):2235–2237
Bruinvels J (1969) Comparison of intracisternally and intraperitoneally injected harmaline on
body temperature and tremor in the rat. J Pharm Pharmacol 21:506–508
Bruneton J (1993) Pharmacognosie: Phytochimie, Plantes Me´dicinales, 2nd edn. Technique et
Documentation, Lavoisier, Paris
Budavari S, O’Neil MJ (1996) The Merck Index, 12nd edn. CRC Press, pp 4644–4645
Burits M, Bucar F (2000) Antioxidant activity of Nigella sativa essential oil. Phytother Res
14:323–328
Burt S (2004) Essential oils: their antibacterial and potential application in foods- A review. Int
J Food Microbiol 94:223–253
Caceres A, Giron LM, Martinez AM (1987) Diuretic activity of plants used for the treatments of
urinary ailments in Guatemaela. J Ethnopharmacol 43:197–201
Caldes G, Prescott B, King JR (1975) Planta Med 27:72–76
Calis I, Kuruϋzϋm-Uz A, Lorenzetto PA, Rϋedi P (2002) (6S)-Hydroxy-3-oxo-α-ionol glucosides
from Capparis spinosa fruits. Phytochemistry 59(4):451–457
Cao YL, Li X, Zheng M (2008) Effect of Capparis spinosa on fibroblast proliferation and type II col-
lagen production in progressive systematic sclerosis. Zhongguo Zhong Yao Za Zhi 33:560–563
Cantrell CL, Rajab MS, Franzblau SG, Fronczek FR, Fisher NH (1999) Antimycobacterial ergos-
terol-5,8-endoperoxide from Ajuga remota. Planta Med 65:732–734
Carling C, Sandberg F (1970) Alkaloids of Haloxylon articulatum. Acta Pharm Suec 7:285–288
Carlini EA, Rodrigues E, Mendes FR, Tabach R, Gianfratti B (2006) Treatment of drug depen-
dence with Brazilian herbal medicines. Rev Bras Farm 16:690–695
Carreiras MC, Rodríguez B, Piozzi F, Savona G, Torres MR, Perales A (1989) A chlorine-
containing and two 17β-neo-clerodane diterpenoids from Teucrium polium subsp. Vincentinum.
Phytochemistry 28(5):1453–1461
Chaieb M, Boukhris M (1998) Flore succinte et illustrée des zones arides et désertiques de Tunisie.
Association pour la protection de la nature et de l’environnement. Sfax, 290 pages
Chalchat JC, Carry RP, Michet A, Benjilali B, Chabart JL (1993) Essential oil of Rosemary
(Rosmarinus officinalis L.), the chemical composition of oil from various origins. J Esent Oil
Res 5:613–618
Chan AS, Yip EC, Yung LY, Pang H, Luk SC, Pang SF, Wong YH (2006) Ckbm stimulates MAPKs
but inhibits LPS-induced IFN-gamma in lymphocytes. Phytother Res 20:725–731
Chao HC, Najjaa H, Villareal MO, Ksouri R, Han J, Neffati M, Isoda H (2013) Arthrophytum
scoparium inhibits melanogenesis through the down-regulation of tyrosinase and melanogenic
gene expressions in B16 melanoma cells. Exp Dermatol 22(2):131–136
Chaouch A (2008a) Chemical composition and antimicrobial activity of the essential oils of
Moroccan Thymus capitatus and Thymus bleicherianus. Phytothérapie 6(6):342–347
Chen KK, Schmidt CF (1930) Ephedrine and related substances. Medicine 9:91–117
Chen CC, Huang YL, Huang FI, Wang CW, Ou JC (2001) Water-soluble glycosides from Ruta
graveolens. J Nat Prod 64(7):990–200
Chen H, Tan RX, Liu ZL, Zhang Y (1996) Antibacterial neoclerodane diterpenoids from Ajuga
lupulina. J Nat Prod 59:668–670
Chevalier A (1935) Les iles du Cap Vert. Flore de l’archipel. Rev Bot Appl Agric Trop 15:733–1090
Chevallier A (1996) The encyclopedia of medicinal plants. Dorling Kindersley, London
Chiu KW, Fung AY (1997) The cardiovascular effects of green beans (Phaseolus aureus), com-
mon rue (Ruta graveolens) and kelp (Laminaria japonica) in rats. Gen Pharmacol 29:859–862
Choi W, Jung H, Kim K, Lee S, Yoon S, Park J, Kim S, Cheon S, Eo W, Lee S (2012) Rhus vernici-
flua stokes against advanced cancer: a perspective from the Korean integrative cancer Center.
J Biomed Biotechnol 2012:181–191
Chopra RN, Chopra IC, Handa KL, Kapur LD (1958) Indigenous drugs of India. Academic
Publishers, Calcutta
Chopra RN, Nayar SL, Chopra IC (1986) Glossary of Indian Medicinal Plants (including the
supplement) Council of Scientific and Industrial Research, New Delhi
Clayton WD (1969) A revision of the Genus Hyparrhenia. Kew Bulletin Additional Series
II. HMSO, London
Coiffard L, Piron-Frenet M, Amicel L (1993) Geographical variations of the constituents of the
essential oil of Crithmum maritimum L. Apiaceae. Int J Cosm Sei 15:15–21
Comte G, Chulia AJ, Vercauteren J, Allais DP (1996) Phenylpropane glycosides from Juniperus
phœnicea. Planta Med 62(1):88–89
Conway GA, Slocumb JC (1979) Plants used as abortifacients and emmenagogues by Spanish
New Mexicans. J Ethnopharmacol 1(3):241–261
Corsi L, Avallone R, Cosenza F, Farina F, Baraldi C, Baraldi M (2002) Antiproliferative effects of
Ceratonia siliqua L. on mouse hepatocellular carcinoma cell line. Fitoterapia 73(7–8):674–684
Cosentino S, Tuberoso CIG, Pisano B, Mascia E, Arzedi E, Palmas F (1999) In-vitro antimicrobial activ-
ity and chemical composition of Sardenian Thymus essential oils. Lett Appl Microbiol 29:130–135
Cuénod A (1954) Flore analytique et synoptique de la Tunisie. Cryptogames vasculaires,
Gymnospermes et Monocotylédones, S.E.F.A.N. (Eds). Tunis, p 287
Cunsolo F, Ruberto G, Amico V, Piattelli M (1993) Bioactive metabolites from Sicilian marine
fennel, Crithmum maritimum. J Nat Prod 56:1598–1600
Dahia M, Laouer H, Chake AN, Prado S, Meierhenrich UJ, Baldovini N (2007) Chemical com-
position and antibacterial activity of Pituranthos chloranthus volatile oil. Nat Prod Commun
2(11):1159–1162
Dapkevicius A, Van Beek VTA, Lelyveld GP, Van Veldhuizen A, DeGroot A, Linssen JPH,
Venskutonis R (2002) Isolation and structural elucidation of radical scavengers from Thymus
vulgaris leaves. J Nat Prod 65(6):892–896
Darwish-Sayed M, Balbaa SI, Afifi MS (1974) The glycosidal content of the different organs of
Citrullus colocynthis. Planta Med 26(3):293–298
Daxenbichler ME, Spencer GF, Carlson DG, Rose GB, Brinker AM, Powel RG (1991) Glucosinolate
composition of seeds from 297 species of wild plants. Phytochemistry 30:2623–2638
De Feo V, De Simone F, Senatore F (2002) Potential allelochemicals from the essential oil of Ruta
graveolens. Phytochemistry 61:573–578
De la Torre M, Rodriguez B, Bruno M, Piozzi F (1988) Montanin C from Teucrium polium var.
album. Fitoterapia 59:129–130
Dellacassa E, Lorenzo D, Moyna P, Frisso CD, Serafini A, Dugo P (1999) Rosmarinus officinalis
L. (Labiatae) essential oils from the south of Brazil and Uruguay. J Esent Oil Res 11(1):27–30
Dendougui H, Seghir S, Belloum Z, Benayache F, Leon F, Brouard I, Bermejo J, Benayache S
(2011) A new labdane diterpene and other constituents from Marrubium deserti Noe ex coss.
Rec Nat Prod 5(4):300–304
Denmeade SR, Isaacs JT (2005) The SERCA pump as a therapeutic target: making a “smart bomb”
for prostate cancer. Cancer Biol Ther 4:14–22
Denmeade SR, Jakobsen CM, Janssen S, Khan SR, Garrett ES, Lilja H, Christensen SB, Isaacs JT
(2003) Prostate-specific antigen-activated thapsigargin prodrug as targeted therapy for prostate
cancer. J Natl Cancer Inst 95:990–1000
Dessí MA, Deiana M, Rosa A, Piredda M, Cottiglia F, Bonsignore L, Deidda D, Pompei R,
Corongiu FP (2001) Antioxidant activity of extracts from plants growing in Sardinia. Phytother
Res 15(6):511–518
Dhief A, Gorai M, Aschi-Smiti S, Neffati M (2009) Comparative phenological and water poten-
tial patterns of three Calligonum species in the eastern great Erg of Tunisia. Flora – Morphol
Distrib Funct Ecol Plant 204(8):581–592
Dhief A, Zouari S, Abdellaoui R, Aschi-Smiti S, Neffati M (2011) Comparative study of chemi-
cal composition of the essential oils from three Calligonum species growing wild in Tunisian
desert. Jeobp 14(1):11–22
Djeridane A, Yousfi M, Nadjemi B, Boutassouna D, Stocker P, Vidal N (2006) Antioxidant activ-
ity of some Algerian medicinal plants extracts containing phenolic compounds. Food Chem
97:654–660
Dob T, Dahmane D, Benabdelkader T, Chelghoum C (2006) Studies on the essential oil composi-
tion and antimicrobial activity of Thymus algeriensis Boiss.et Reut. Int J Aromather 16:95–100
Domokos J, Hethelyi E, Palinkas J, Szirmai S, Tulok HM (1997) Essential oil of rosemary
(Rosmarinus officinalis L) of Hungarian origin. J Esent Oil Res 9:41–45
Dorman HJD, Deans SG (2000) Antimicrobial agents from plants: antibacterial activity of plant
volatile oils. J Appl Microbiol 88:308–316
Dorman HJD, Deans SG (2004) Chemical composition, antimicrobial and in vitro antioxidant
properties of Monaranda citriodora var. citriodora, Myristica fragrans, Origanum vulgare ssp.
hirutum, pelargonium sp. and Thymus zygis oils. J Essent Oil Res 16:145–150
Durenkamp M, Dekok LJ (2004) J Exp Bot 55(404):1821–1830
Dziri S, Hassen I, Fatnassi S, Mrabet Y, Casabianca H, Hanchi B, Hosni K (2012) Phenolic con-
stituents, antioxidant and antimicrobial activities of rosy garlic (Allium roseum var. odoratis-
simum). J Funct Foods 4(2):423–432
Eddouks M, Maghrani M, Lemhadri A, Ouahidi ML, Jouad H (2002) Ethnopharmacological sur-
vey of medicinal plants used for the treatment of diabetes mellitus, hypertension and cardiac
diseases in the south-east region of Morocco (Tafilalet). J Ethnopharmacol 82:97–103
Eddouks M, Lemhadri A, Michel JB (2004) Caraway and caper: potential antihyperglycaemic
plants in diabetic rats. J Ethnopharmacol 94:143–148
Eddouks M, Lemhadri A, Michel JB (2005) Hypolipidemic activity of aqueous extract of Capparis
spinosa L. in normal and diabetic rats. J Ethnopharmacol 98:345–350
Edziri H, Ammar S, Groh P, Mahjoub MA, Mastouri M, Gutmann L, Zine M, Aouni M (2007)
Antimicrobial and cytotoxic activity of Marrubium alysson and Retama retama in Tunisia. Pak
J Biol Sci 10:1759–1762
Edziri H, Mastouri M, Ammar S, Matieu M, Patrich G, Hiar R, Mahjoub MA, Ali SM, Laurent G,
Mighri Z (2008) World. J Microbiol Biotechnol 24(12):2933–2940
Edziri H, Mastouri M, Chéraif I, Aouni M (2010) Chemical composition and antibacterial, antifun-
gal and antioxidant activities of the flower oil of Retama raetam (Forssk.). Webb Tunisia Nat
Prod Res Form Nat Prod Lett 24(9)
Ehab AA, El-Alfy AT, Khan IA, Walker L (2003) Ephedra in perspective – a current review.
Ehivet FE, Min B, Park MK, Oh JH (2011) Characterization and antimicrobial activity of sweet
potato starch based edible film containing origanum (Thymus capitatus) oil. J Food Sci
76:178–184
Elamrani A, Zrira S, Benjilali B, Berrada MA (2000) Study of Moroccan Rosemary oils. J Esent
Oil Res 12:487–495
El Agraa SE, El Balwi SM, Adam SE (2002) Preliminary observations on experimental Ruta gra-
voelens toxicosis in Nubian goats. Trop Animal Health Proc 34:271–281
El Ajjouri M, Satrani B, Ghanmi M, Aafi A, Farah A, Rahouti M, Amarti F, Aberchane M (2008)
Activité antifongique des huiles essentielles de Thymus bleicherianus Pomel et Thymus
capitatus (L.) Hoff.& Link contre les champignons de pourriture du bois d’eouvre. BASE
12(4):345–351
El Amrani F, Rhallab A, Alaoui T, El Badaoui K, Chakir S (2009) Hypoglycaemic effect of T. hir-
suta in normal and streptozotocininduced diabetic rats. J Med Plant Res 3:625–629
El-Demerdash FM, Yousef MI, Abou El-Naga NI (2005) Food Chem Toxicol 43(1):57–63
El-Domiaty MM, El-hafae AM, Abdel-Aal MM (1997) Alexandria J Pharmaceut Sci 11:13
El Hilaly J, Lyoussi B (2002) Hypoglycemic effect of the lyophilized aqueous extract of Ajuga iva
in normal and streptozotocin diabetic rats. J Ethnopharmacol 80:109–113
El-Hilaly J, Tahraoui A, Israili ZH, Lyoussi B (2006) Hypolipidemic effects of acute and sub-
chronic adminis-tration of an aqueous extract of Ajuga iva L. whole plant in normal and dia-
betic rats. J Ethnopharmacol 105:441–448
El-Rhaffari L, Zaid A (2002) Pratique de la phytothérapie dans le sud-est du Maroc (Tafilalet): Un
savoir empirique pour une pharmacopée rénovée. Des sources du savoir aux médicaments du
futur 1:293–318
Esmaeili MA, Yazdanparast R (2004) Hypoglycaemic effect of Teucrium polium: studies with rat
pancreatic islets. J Ethnopharmacol 95:27–30
Es-Safi NE, Khlifi S, Kollmann A, Kerhoas L, El Abbouyi A, Ducrot PH (2006) Iridoid glucosides
from the aerial parts of Globularia alypum L. (Globulariaceae). Chem Pharm Bull 54:85–88
Es-Safia NE, Kollmann A, Khlifi S, Ducrot PH (2007) Antioxidative effect of compounds isolated
from Globularia alypum L. structure – activity relationship. LWT 40:1246–1252
Essawi T, Srour M (2000) Screening of some Palestinian medicinal plants for antibacterial activity.
Facciola S (1990) Cornucopia – a source book of edible plants. Kampong Publications, Vista
Faust RE, Cwalina G, Ramstad E (1958) The antineoplastic action of chemical fractions of the fruit
of Citrullus colocynthis on sarcoma-37. J Am Pharm Assoc 47(1):1–5
Feuerstein I, Müller D, Hobert K, Danin A, Segal R (1986) The constitution of essential oils from
Artemisia herba-alba populations of Israel and Sinaï. Phytochemistry 25:2343–2347
Fici S (2001) Intraspecific variation and evolutionary trends in Capparis spinosa L. (Capparaceae).
Plant Syst Evol 228(3–4):123–141
Figliuolo G, Mang SM (2010) Characterization and discrimination of Mediterranean bulb-
producing garlic. In: Acurar MP, Krejci G (eds) Garlic consumption and health. Nova Science
Publishers, Inc., New York, pp 181–197
Figueiredo AC, Barroso JG, Pedro LG (2008) Tymus capitatus grown in Tunisia: antioxidant abil-
ity of the essential oils on lilnoleic acid evaluated by different methods. Acta Hortic (ISHS)
765:315–324
Food and Drug Administration (FDA) Center for Food Safety and Applied Nutrition (1994)
Adverse events with Ephedra and other botanical dietary supplements
Fragiska M (2005) Wild and cultivated vegetables, herbs and spices in Greek antiquity. Environ
Archaeol 10(1):73–82
Freitag H, Maier-Stolte M (1989) The Ephedra species of P. Forsskal: identity and typification.
Taxon 38:545–556
Freitag H, Maier-Stolte M (1994) Ephedraceae. In: Browicz K (ed) Chorology of trees and shrubs
in south-west Asia and adjacent regions. Bogucki Publishers, Poznan
Gachkar L, Yadegari D, Rezaei MB, Taghizadeh M, Astaneh SA, Rasooli I (2007) Chemical and
biological characteristics of Cuminum cyminum and Rosmarinus officinalis essential oils. Food
Chem 102:898–904
Gadgoli C, Mishra SH (1999) Antihepatotoxic activity of p-methoxy benzoic acid from Capparis
spinosa. J Ethnopharmacol 66:187–192
Galati EM, Mondello MRD, Aquino A, Miceli N, Sanogo R, Tzakou O (2000) Effects of Teucrium
divaricatum Heldr. ssp. divaricatum decoction on experimental ulcer in rats. J Ethnopharmacol
72:337–342
Gao R, Gao C, Tian X, Yu X, Di X, Xiao H, Zhang X (2004) Insecticidal activity of deoxypodo-
phyllotoxin, isolated from Juniperus sabina L, and related lignans against larvae of Pieris rapae
L. Pest Manag Sci 60:1131–1136
Germano MP, De Pasquale R, D’Angelo V, Catania S, Silvaria V, Costa C (2002) Evaluation of
extracts and isolated fraction from Capparis spinosa L. buds as an antioxidant source. J Agric
Food Chem 50:1168–1171
Gharaibeh MN, Elayan HH, Salhab AS (1988) Hypoglycemic effects of Teucrium polium.
J Ethnopharmacol 24(1):93–99
Ghazal S, Abuzarqa MA, Mahasneh MA (1992) Antimicrobial activity of Polygonum equiseti-
forme extracts and flavonoids. Phytother Res 6(5):265–269
Ghedira K, Chemli R, Richard B, Nuzillard JM, Zeches M (1993) Two cyclopeptide alkaloids from
Zizyphus lotus. Phytochemistry 32(6):1591–1594
Giancarlo S, Rosa LM, Nadjafi F, Francesco M (2006) Hypoglycaemic activity of two spices
extracts: Rhus coriaria L. and Bunium persicum Boiss. Nat Prod Res 20:882–886
Gil de Meister E, Hoffman FR (1956) Die Atherischen Oll le, vol 4. Akademie-Verlag, Berlin,
pp 296–297
Giordani R, Hadef Y, Kaloustian J (2008) Compositions and antifungal activities of essential oils
of some Algerian aromatic plants. Fitoterapia 79:199–203
Girre L (2000) Infusions et Plantes de Santé en France. Ed. Tours, Ouest-France, p 60
Glasby JS (1978) Encyclopedia of the alkaloids. Plenum Press, London, pp 58–661
Gomes I, Leyens T, Da Luz B, Costa J, Gonçalves F (1999) New data on the distribution and
conservation status of some angiosperms of the Cape Verde Islands, W Africa. Willdenowia
29:105–114
Goodner KL, Mahattanatawee K, Plotto A, Sotomayor JA (2006) Aromatic profiles of Thymus hye-
malis and Spanish T. vulgaris essential oils by GC-MS/GC-O. Ind Crop Prod 24(3):264–268
Gorai M, Maraghni M, Neffati M (2010) The relationship between phenological traits and water
potential patterns of the wild jujube Ziziphus lotus in southern Tunisia. Plant Ecol Divers
3:273–280
Goren AC, Bilsela G, Bilsela M, Demira H, Kocabas EE (2003) Analysis of essential oil of cori-
dothymus capitatus (L.) and its antibacterial and antifungal activity. Z Naturfo B 58C:687–690
Granger R, Passet J (1973) Thymus vulgaris spontané de France: Races chimiques et chemotax-
onomie. Phytochemistry 12(7):1683–1691
Grim F, Bounaga N (1996) Flavonic polymorphism and chemosystematics of the genus
Zygophyllum studied in the Algerian Sahara. Acta Pharma 46:187–194
Guarrera PM (1999) Traditional anthielmintic, antiparasitic and repellent uses of plants in Central
Italy. J Ethnopharmacol, 68(1–3):183–192.
Guenaoui C, Mang S, Figliuolo G, Neffati M (2012) Diversity in Allium ampeloprasum: from
small and wild to large and cultivated. Genet Resour Crop Evol 53:19–25
Guenther E (1950) The essential oils, 3rd edn. D. Van Nostrand, New York
Guetat A, Vilatersana R, Neffati M, Bousaid M (2010) Genetic diversity in Tunisian rosy garlic
populations (Allium roseum L.) as evidenced by chloroplastic DNA analysis: Sequence varia-
tion of non-coding region and intergenic spacers. Biochem Syst Ecol 38(4):502–509
Gurley BJ, Wang P, Gardner SF (1998) Ephedrine-type alkaloid content of nutritional supplements
containing Ephedra sinica (Ma-huang) as determined by high performance liquid chromatog-
raphy. J Pharm Sci 87:1547–1553
Haber B (2002) Carob fibre benefits and applications. Cereal Foods World 47:365–369
Hacıseferoğullari H, Őzcan M, Demir F, Çalışır S (2005) Some nutritional and technological prop-
erties of garlic (Allium sativum L.) J Food Eng 68:463–469
Hajji M, Masmoudi O, Ellouz-Triki Y, Siala R, Gharsallah N, Nasri M (2009) Chemical composi-
tion and antioxidant and radical-scavenging activities of Periploca laevigata root bark extracts.
J Sci Food Agric 89:897–905
Hajji M, Masmoudi O, Triki Y, Souissi N, Kammoun S, Nasri M (2010) Chemical composition,

angiotensin I-converting enzyme (ACE) inhibitory, antioxidant and antimicrobial activities of
the essential oil from Periploca laevigata root barks. Food Chem 121(3):724–731
Haller CA, Benowitz NL (2000) Adverse cardiovascular and central nervous system events associ-
ated with dietary supplements containing ephedra alkaloids. N Engl J Med:3431833–3431838
Hamilton AC, Hamilton PB (2006) Plant conservation: an ecosystem approach. Earthscan, London
Hammami R, Zouhir A, Ben Hamida J, Neffati M, Vergoten G, Naghmouchi K, Fliss I (2009)
Antimicrobial properties of aqueous extracts from three medicinal plants growing wild in arid
regions of Tunisia. Pharm Biol 47(5):452–457
Han BH, Park MH (1986) Folk medicine: the art and science. The American Chemical Society,
Washington, p 205
Harborne JB (1969) Chemosystematics of the leguminosae. Flavonoid and Isoflavonoid patterns in
the tribe genisteae. Phytochemistry 8(8):1449–1456
Harborne JB, Mabry TJ, Mabry H (1975) The Flauonoids. Chapman & Hall, London
Hardman JG, Limbird LE (2001) The pharmacological basis of therapeutics, 10th edn. McGraw-
Hill, New York
Hartwell JL (1982) In plant used against cancer a survey. Quarterman Publication, Inc., Lawrence
Hashem FA, Saleh MM (1999) Antimicrobial components of some cruciferae plants (Diplotaxis
harra Forsk. and Erucaria microcarpa Boiss.) Phytother Res 13:329–332
Hassan MM, Muhtadi FJ, Al-Badr AA (1979) Antispasmodic GLC-mass spectrometry of Teucrium
polium oil. J Pharm Sci 68:800–801
Hassanean HA, El-Hamouly MMA, El-Moghazy SA, Bishay DW (1993) 14-decarboxyquinovic
and quinovic acid glycosides from Zygophyllum album. Phytochemistry 33:667–670
Hassanien Bey AM (1925) The lost oases. Century Co., New York/London. pp xxiii, 363
Hassar M (1999) La phytothérapie au Maroc. Espérance Médicale 47:83–85
Hatimi S, Boudouma M, Bichichi M, Chaib N, Idrissi NG (2000) Evaluation in vitro of antileish-
manien activity of Artemisia herba-alba Asso. (in French). Presented at Franco-African meet-
ing of pediatrics N°14, Paris, France. Exotic pathology Society, Paris, pp 57–70
Hatimi S, Boudouma M, Bichichi M, Chaib N, Idrissi NG (2001) Bull Soc Pathol Exot 94:29
Havey MJ (1999) Advances in new Alliums. In: Janick J (ed) Perspectives on new crops and new
uses. ASHS Press, Alexandria
Havsteen B (1983) Flavonoids, a class of natural products of high pharmacological potency.
Biochem Pharmacol 32:1141–1148
Hayouni E, Abedrabba M, Bouix M, Hamd M (2007) The effects of solvents and extraction method
on the phenolic contents and biological activities in vitro of Tunisian Quercus coccifera L. and
Juniperus phoenicea L. fruit extracts. Food Chem 105:1126–1134
Hazzit M, Baaliouamer A, Verıssimo AR, Faleiro ML, Miguel MG (2009) Chemical composition
and biological activities of Algerian Thymus oils. Food Chem 16:714–721
Hetwood VH (1971) The biology and chemistry of the Umbelliferae. Academic Press, New York
Hooper D (1937) Useful plants and drugs of Iran and Iraq. Botanical series, Publication 387, Field
Museum of Natural History, Chicago, p 94
Horbowicz M, Kotlińska T (2000) Level of flavonols in wild and cultivated Allium species. Acta
Hortic 517:375–380
Hoult JRS, Moroney MA (1994) Paya, M. Methods Enzymol 234:443
Hudaib M, Aburjai T (2006) Composition of the Essential Oil from Artemisia herba-alba Grown
in Jordan. J Essent Oil Res 18:301–304
Hudaib M, Speroni E, Di Pietra AM, Cavrini V (2002) GC/MS evaluation of thyme (Thymus vul-
garis L.) oil composition and variations during the vegetative cycle. J. Pharmaceut. Biomed.
Analysis 29(4):691–700
Iauk L, Mangano K, Rapisarda A, Maiolino L, Musumeci R, Costanzo R, Serra A (2004)
Protection against murine endotoxemia by treatment with Ruta chalepensis L., a plant with
anti-inflammatory properties. J Ethopharmacol 90:267–272
Infantino A, Tomassoli L, Peri E, Colazza S (2007) Viruses, fungi and insect pests affecting caper.
Eur J Plant Sci Biotechnol 1(2):170–179
Inocencio C, Rivera D, Alcaraz F, Tomas-Barberan FA (2000) Flavonoid content of commercial

capers (Capparis spinosa, C. sicula, and C. orientalis) produced in Mediterranean countries.
Eur. Food Res. Dent Tech 212:70–74
Inocencio C, Alcaraz F, Calderón F, Obón C, Rivera D (2002) The use of floral characters in
Capparis sect. Capparis’ to determine the botanical and geographical origin of capers. Eur
Food Res Technol 214(4):335–339
Inocenio C, Rivera D, Obon MC, Alcaraz F, Barrena JA (2006) A systematic revision of Capparis
section Capparis (Capparaceae). Ann Mo Bot Gard 93:122–149
Ismaili H, Tortora S, Sosa S, Fkih-Tetouani S, Ilidrissi A, Della Loggia R, Tubaro A, Aquino
R (2001) Topical anti-inflammatory activity of Thymus willdenowii. J Pharm Pharmacol
53:1645–1652
Ismaili H, Sosa S, Brkic D, Fkih-Tetouani S, Ilidrissi A, Touati D, Aquino R, Tubaro A (2002)
Topical anti-inflammatory activity of extracts and compounds from Thymus broussonettii.
J Pharm Pharmacol 54:1137–1140
Ismaili H, Milella L, Fkih-Tetouani S, Ilidrissi A, Camporese A, Sosa S, Altinier G, Della Loggia
Aquino R (2004) In vivo topical anti-inflammatory and in vitro antioxidant activities of two
extracts of Thymus satureioides leaves. J Ethnopharmacol 91:31–36
Isman MB, Wilson JA, Bradbury R (2008) Insecticidal activities of commercial rosemary oils
(Rosmarinus officinalis L.) against Trichoplusiani in relation to their chemical compositions.
Pharm Biol 46(1–2):82–87
Itokawa H, Xu JP, Takeya K (1988) Studies on chemical constituents of antitumor fraction from
Periploca sepium. IV. Structures of new pregnane glycosides, periplocosides D, E, L, and
M. Chem Pharm Bull 36:2084–2089
IUCN (2005) A guide to medicinal plants in North Africa. ISBN: 2, 8317-0893-1. p 256
Jaafari A, Ait Mouse H, El Rakib M, Ait L, Barek M, Tilaoui M, Benbakhta C, Boulli A, Abbad
A, Zyad A (2007) Chemical composition and antitumor activity of different wild varieties of
Moroccan thyme. Rev Bras Farm 17(4)
Jafri SMH (1977) Rhamnaceae. In: Jafri SMH, EL-Gadi A (eds) Flora of Libya vol 30. Al-Faateh
University, Department of Botany, Tripoli, pp 1–13
Jager AK, Schottlander S, Smitt UW, Nyman U (1993) Somatic embryogenesis in cell cultures of
Thapsia garganica: correlation between the state of differentiation and the content of thapsi-
gargins. Plant Cell Rep 12:517–520
Jagtap SD, Deokule SS, Bhosle SV (2006) Ethnopharmacological communication some unique
ethnomedicinal uses of plants used by the Korku tribe of Amravati district of Maharashtra.
India J Ethnopharmacol 107(03):463–469
Jamshidi R, Afzali Z, Afzali D (2009) Chemical composition of hydrodistillation essential oil of
rosemary in different origins in Iran and comparaison with other countries. American-Eurasian
J Agr Envriron Sci 5(1):78–81
Janick J, Paull RE (2006) The encyclopedia of fruits and nuts. Edited by Janick J, Purdue University,
USA, and Paull RE., University of Hawaii at Manoa, USA.; pp 228–231
Janssen MA, Scheffer JJC, Svendsen AB (1987) Antimicrobial activities of essential oils: a
1976–1986 literature review on possible applications. Pharmaceutische Weekblad (Scient Edn)
9:193–197
Jaouhari JT, Lazrek HB (2000) Hypoglycemic activity of Zygophyllum gaetulum extracts in
alloxan induced hyperglycemic rats. J Ethnopharmacol 69(1):17–20
Jaouhari JT, Lazrek HB, Seddik A, Jana M (1999) Hypoglycaemic response to Zygophyllum
gaetulum extracts in patients with non-insulin-dependent diabetes mellitus. J Ethnopharmacol
64(3):211–217
Jarraya R, Damak M (2001) Alcaloïdes des feuilles de Hammada scoparia (pomel) Iljin. J de la
Société Chimique de Tunisie 4(9):941–948
Jarraya R, Chaieb M, Damak M (1993) Screening des plantes à alcaloïdes au sein de la flore
Tunisienne. Plant Med Phytother 26:177–189
Jarraya MR, Bouaziz A, Hamdi B, Ben Salah A, Damak M (2008) N-methylisosalsoline from
Hammada scoparia. Acta Cryst E 64:1710–1714
Jones MG, Hughes J, Tregova A, Mline J, Tomsett AB, Collin HA (2004) J Exp Bot
55(404):1903–1918
Josefson D (1995) Herbal stimulant causes US deaths. Br Med J 312:1378–1379
Jouad H, Haloui M, Rhiouani H, ElHilaly J, Eddouks M (2001) Ethnobotanical survey of medici-
nal plants used for the treatment of diabetes, cardiac and renal diseases in the North centre
region of Morocco (Fez-Boulemane). J Ethnopharmacol 77:175–182
Joyeux M, Lobsrein A, Anton R, Mortier F (1995) Planta Med 61:126
Kamel S, Ibrahim L, Afifi A, Hamza (1970) Major alkaloidal constituents of the Egyptian plant.
Peganum harmala. UARJ Vet Sci 7:71–86
Kanat M, Alma MH (2004) Insecticidal effects of essential oils from various plants against larvae of
pine processionary moth (Thaumetopoea pityocampa Schiff) (Lepidoptera: Thaumetopoeidae).
Pest Manag Sci 60:173–177
Karaman I, Sahin F, Gulluce M, Ogutcu H, Sengul M, Adiguzel A (2003) Antimicrobial activity
of aqueous and methanol extracts of Juniperus oxycedrus L. J Ethnopharmacol 85:231–235
Kariba RM (2001) Antifungal activity of Ajuga remota. Fitoterapia 72:177–178
Karousou R, Koureas DN, Kokkini S (2005) Essential composition is related to the natural
habitats: Coridothymus capitatus and satureja thymbra in NATURA 2000 sites of Crete.
Phytochemistry 66:2668–2673
Kassem M, Mosharrafa S, Saleh N (2000) Two new flavonoids from Retama raetam. Fitoterapia
71:649–654
Kawano M, Matsuyama K, Miyamae Y, Shinmoto H, Kchouk ME, Morio T, Shigemori H, Isoda
H (2007) Antimelanogenesis effect of Tunisian herb Thymelaea hirsuta extract on B16 murine
melanoma cells. Exp Dermatol 16(12):977–984
Kazmi SU, Ali SN, Jamal SA, Attaur R (1991) New bioactive compounds of plant origin. Pak
J Pharm Sci 4(2):113–123
Ketoh GK, Glitho AI, Huignard J (2002) Susceptibility of the bruchid Callosobruchus maculates
(Coleoptera: Bruchidae) and its parasitoid Dinarmus basalis (Hymenoptera: Pteromalidae) to
three essential oils. J Econ Entomol 95(1):174–182
Keusgen M, Jinger M, Krest I, Schoning MJ (2003) Sensors Actuators B 95:297–302
Khadri A, Serralheiro MLM, Nogueira JMF, Neffati M, Smiti S, Araujo MEM (2008) Antioxidant
and antiacetylcholinesterase activities of essential oils from Cymbopogon schoenanthus
L. Spreng. Determination of chemical composition by GC–mass spectrometry and C NMR13.
Food Chem 109:630–637
Khadri A, Neffati M, Smiti S, Fale P, Rosa A, Lino L, Luisa M, Serralheiro M, Eduarda M, Araujoc
M (2010) Antioxidant, antiacetylcholinesterase and antimicrobial activities of Cymbopogon
schoenanthus L. Spreng (lemon grass) from Tunisia. LWT Food Sci Technol 43:331–336
Khafagi IK, Dewedar A (2000) The efficiency of random versus ethno-directed research in the
evaluation of Sinai medicinal plants for bioactive compounds. J Ethnopharmacol 71:365–376
Khakberdyev MM, Mansurov MM, Eshchanov TB (1968) Russian Medskii Zh. Uzbek 12:47–48
(Biol Abstr 50:109453)
Khouri NA, El-Akawi Z (2005) Anti-androgenic activity of Ruta graveolens L. in male albino rats
with emphasis on sexual and aggressive behavior. Neuroendocrinol Lett 26:823–829
Kiritikar KR, Basu BD (1987) Indian medicinal plants, vol 1. International Book Distributors,
Dehradun, pp 195–197
Kiritikar KR, Basu BD (1991) Indian medicinal plants. Periodical Expert Book Agency, Delhi,
pp 197–198
Kjaer A, Thomsen H (1963) Isothiocyanate-producing glucosides in species of Capparidaceae.
Koba K, Poutouli PW, Nenonene YA, Songai MS, Raynaud C, Sanda K (2007) Chemical com-
position and anti-termite activity of three tropical essential oils against termite species
Trinervitermes geminates (Wasmann). J Sci Technol 5(2):39–46
Kokwaro JO (1976) Medicinal plants of East Africa. East African Literature Bureau, Nairobi, p 106
Kubo I, Klocke JA, Asano S (1981) Insect ecdysis inhibitors from the East African medicinal plant,
Ajuga remota (Labiatae). Agric Biol Chem 45:1925–1927
Kumarasamy Y, Cox PJ, Jaspars M, Nahar L, Sarker SD (2002) Screening seeds of Scottish plants
for antibacterial activity. J Ethnopharmacol 83:73–77
Kumazawa S, Taniguchi M, Susuki Y, Shimura M, Kwon MS, Nakayama T (2002) Antioxidant
activity of polyphenols in carob pods. J Agric Food Chem 50:373–377
Kuria KAM, Muriuki G (1984) A new cardiotonic agent from Ajuga remota benth (Labiatae). East
Afr Med J 61:533–538
Kuria KAM, Chepkwony H, Govaerts C, Roets E, Busson R, de Witte P, Zupko I, Hoornaert G,
Quirynen L, Maes L (2002) The antiplasmodial activity of isolates from Ajuga remota. J Nat
Prod 65:789–793
Kuria KAM, De Coster S, Muriuki G (2001) Antimalarial activity of Ajuga remota benth (Labiatae)
and Caesalpinia volkensii Harms (Caesalpiniaceae): in vitro confirmation of ethnopharmaco-
logical use. J Ethnopharmacol 74:141–148
Kusmenoglu S (1996) The plant Peganum harmala L. and its biologically active constitutents.
FABAD J Pharm Sci 21:71–75
Kutepova TA, Syrov VN, Khushbaktova ZA, Saatov Z (2001) Hypoglycemic activity of the total
ecdysteroid extract from Ajuga turkestanica. Pharm Chem J 35:608–609
Laamouri A, Zine El Abidine A (2000) Multiplication des jujubiers en Tunisie. Annales de la
Recherche Forestière au Maroc 33:37–49
Lahlou M, El Mahi M, Hamamouchi J (2002) Évaluation des activités antifongique et molluscicide
de Zizyphus lotus (L.) Desf. du Maroc. Ann Pharm Fr 60(6):410–414
Lam SK, Ng TB (2009) A protein with antiproliferative, antifungal and HIV-1 reverse transcriptase
inhibitory activities from caper (Capparis spinosa) seeds. Phytomedicine 16:444–445
Lam SK, Han QF, Ng TB (2009) Isolation and characterization of a lectin with potentially exploit-
able activities from caper (Capparis spinosa) seeds. Biosci Rep 29(5):293–299
Lamchouri F, Settaf A, Cherrah Y, Zemzami M, Lyoussi B, Zaid A, Hassar M (1999) Antitumor
principles from Peganum harmala seeds. Therapie 54:753–758
Lamchouri F, Settaf A, Cherrah Y, Hassar M, Zemzami M, Atif N, Lyoussi B (2000) In vitro cell-
toxicity of Peganum harmala alkaloids on cancerous cell-lines. Fitoterapia 71:50
Lamiri A, Belanger A, Berrada M, Ismaïli-Alaoui MM, Benjilali B (1997a) Origin of chemical
polymorphism of Moroccan Artemisia herba-alba Asso (in French). Rabat, Morocco, pp 81–92
Lamiri A, Belanger A, Berrada M, Zrira S, Benjilali B (1997b) Chemical polymorphism of
Artemisia herba-alba Asso from Morocco (in French). Rabat, Morocco, pp 69–79
Lang D (2008) Trade in plant material for medicinal and other purposes. TRAFFIC Bull 17:21–32
Larsen HO, Smith LK (2004) Alpine medicinal plant trade and mountain livelihood strategies.
Geogr J 169(3):243–254
Lawrence BM (2003) Progress in essential oils. Perfum Flav 28(2):62
Le Croueour G, Thepenier P, Richard B, Petermann C, Ghedira K, Zeches-Hanrot M (2002)
Lotusine G: a new cyclopeptide alkaloid from Zizyphus lotus. Fitoterapia 73:63–68
Le Floc’h E (1983) Contribution à une étude ethnobotanique de la flore tunisienne. Programme
Flore et Végétation tunisienne. Ed., Ministère de l’Enseignement Supérieur et de la Recherche
Scientifique, Tunis, pp 83–85
Le Floch E, Boulos L (2008) Flore de la Tunisie. Catalogue Synonymique Commente´,
Montepellier, p 461
Le Houérou HN (1959) Recherches écologiques et floristiques sur la végétation de la Tunisie méri-
dionale. Institut des Recherches Sahariennes, Algérie
Le Houerou HN (1969) La vegetation de la Ttunisie steppique (avec references au Maroc, en
Algérie et la Libye). Ann Inst Nat Rech Agric Tunisie 42:642p
Lee JC, Lee KY, Kim JN (2002) Identification of Rhus verniciflua Stokes compounds that exhibit
free radical scavenging and antiapoptotic properties. Biochim Biophys Acta 1570:181–191
Lee JC, Lee KY, Kim JN (2004) extract from Rhus verniciflua Stokes is capable of inhibiting the
growth of human lymphoma Murray cells. Food Chem Toxicol 9:1383–1388
Lei H, Wang B, Li WP, Yang Y, Zhou AW, Chen MZ (2003) Anti-ageing effect of astragalo-sides
and its mechanism of action. Acta Pharmacol Sin 24:230–234
Lentini F, Aleo M, Amenta R (1993) L’uso popolare delle piantenelle Isole Egadi (Sicilia). G Bot
Ital 127(3):702
Leporatti ML, Ghedira K (2009) Comparative analysis of medicinal plants used in traditional
medicine in Italy and Tunisia. J Ethnobiol Ethnomed 5:31
Leung AY, Foster S (1996) Encyclopedia of common natural ingredients. John Wiley & Sons,
New York
Leung AY (1999) Ephedrine, Ephedra, Mahuang, Mahuanggen what are they? Presented at AHPA
Ephedra international symposium, Arlington, VA
Levizon E, Drilias P, Kyparissis A (2004) Exceptional photosynthetic performance of Capparis
spinosa L. under adverse conditions of Mediterranean summer. Photosynthetica 42:229–235
Li JW, Ding SD, Ding XL (2005) Comparison of antioxidant capacities of extracts from five culti-
vars of Chinese jujube. Process Biochem 40(11):3607–3613
Lin YM, Flavin MT, Schure R, Chen FC, Sidwell R, Barnard DL (1999) Antiviral activities of
bioflavonoids. Planta Med 65:120–125
Liu MJ, Cheng CY (1994) A taxonomic study on Chinese jujube and wild jujube. J Agr Univ
Hebei 17:251–263
Liu XM, Zakaria MN, Islam MW, Radhakrishnan R, Ismail A, Chen HB, Chan K, Al-Attas A
(2001) Anti-inflammatory and anti-ulcer activity of Calligonum comosum in rats. Fitoterapia
72(5):487–491
Loeb H, Vandenplas Y, Wursch P, Guesry P (1989) Tannin-rich carob pod for the treatment of
acute-onset diarrhea. J Paediatr Gastroenterol Nutr 8(4):480–485
Lograda T, Ramdani M, Figueredo G, Chalchat JC, Chalard P (2012) Essential oil composition and
antimicrobial activity of Genista microcephala Coss. et Dur. Int J Med Arom Plants 2(1):75–79
Mabberley DJ (1997) The plant-book. Cambridge University Press, Cambridge
Mahdhi M, Nzoué A, Gueye F, Merabet C, De Lajudie P, Mars M (2007) Phenotypic and genotypic
diversity of Genista saharae microsymbionts from the infra-arid region of Tunisia. Lett Appl
Microbiol 45(6):604–609
Mahjoub MA, Ammar S, Edziri H, Mighri N, Bouraoui A, Mighri Z (2004) Anti-inflammatory and
antioxidant activities of some extracts and pure natural products isolated from Rhus tripartitum.
J Soc Alger Chim 14:293–297
Mahjoub MA, Ammar S, Mighri Z (2005) A new biflavonoid and an isobiflavonoid from Rhus
tripartitum. Nat Prod Res Form Nat Prod Lett 19(8)
Mahjoub MA, Ammar S, Edziri H, Mighri N, Bouraoui A, Mighri Z (2010) Anti-inflammatory and
antioxidant activities of some extracts and pure natural products isolated from Rhus tripartitum
(Ucria). Med Chem Res 19(3):271–282
Mahmoudian M, Jalilpour H, Salehian P (2002) Toxicity of Peganum harmala: review and a case
report. Iran J Pharmacol Ther 1:1–4
Maire R (1962) Flore de l’Afrique du Nord, vol 8, ed. Paul Lechevalier, Paris, pp 161–164
Maire R (1987) Flore de l’Afrique du Nord, Dicotyledonae, ed. Lechevalier, vol 16. Paris, p 302
Maiza K, Brac de la Perrière RA, Hammiche V (1993) Traditional Saharian pharmacopoeia. Acta
Hortic 332:37–42
Makris DP, Kefalas P (2004) Carob pods (Ceratonia siliqua L.) as a source of polyphenolic anti-
oxidants. Food Technol Biotechnol 42:105–108
Mangena T, Muyima NYO (1999) Comparative evaluation of the antimicrobial activities of essen-
tial oils of Artemisia afra, Pteronia incana and Rosmarinus officinalis on selected bacteria and
yeast strains. Lett Appl Microbiol 28:291–296
Marakis S (1996) Carob bean in food and fee: current status and future potentials- a critical
appraisal. J Food Sci Technol 33:365–383
Marakis S, Marakis G, Lambraki M (1997) Tannins of eight carob varieties from the island of
Lefkada, Greece, Chimica Chronika. New Series 26:57–66
Marie R (1964) Flore de l’Afrique du Nord (Maroc, Algérie, Tunisie, Tripolitaine cyrénaïque et
sahara). Paris XIéme, p 156
Marrif HI, Ali BH, Hassan KM (1995) Some pharmacological studies on Artemisia herba-alba
(Asso.) in rabbits and mice. J Ethnopharmacol 49(1):51–55
Marti D, Villagrasa V, Martinez-Solis I, Blanquer A, Castillo E, Moreno Royo L (2005) Hystological
and pharmacological study of Thymus piperella (L.) Phytother Res 19:298–302
Martins A, Wink M, Tei A, Brum-Bousquet M, Tillequin F, Rauter P (2005) A phyto-chemical
study of the quinolizidine alkaloids from Genista tenera by gas chromatography–mass spec-
trometry. Phytochem Anal 16:264–266
Mason M (1936) The paradise of fools. Hodder and Stoughton, London, 282 pp
Mastelic J, Kustrak D (1997) Essential oil and glycosidically bound volatiles in aromatic plants.
II. Rosemary (Rosmarinus officinalis L., Lamiaceae). Acta Pharmaceut 47(2):139–142
Matsuyama K, Villareal MO, El Omri A, Han J, Kchouk ME, Isoda H (2009) Effect of Tunisian
Capparis spinosa L. extract on melanogenesis in B16 murine melanoma cells. J Nat Med
63:468–472
Mbark AN, Charrouf Z, Wieruszeki JM, Leroy Y, Kol O (1995) Herniaria Saponin A, A Novel
Saponin from Herniaria Fontanesii. Nat Prod Lett 6(3)
Mbark AN, Guillaume D, Cherrah Y, Wieruszeki JM, Ricart G, Charrouf Z (1996) An acylated
isorhamentin glycoside from Herniaria fontanesii. Phytochemistry 41(3):989–990
Mbark AN, Charrouf Z, Guillaume D, Kol O (1999) New glycosidesfrom Herniaria fontanesii.
Stud Plant Sci 6:314–319. Adv Plant Glycos Chem Biol. Proceedings of the international sym-
posium in plant glycosides
McCutcheon AR, Ellis SM, Hancock RE, Towers GH (1992) Antibiotic screening of medicinal
plants of the British Columbian native peoples. J Ethnopharmacol 37:213–223
Meister A, Bernhardt G, Christoffel V, Buschauer A (1999) Antispasmodic activity of Thymus
vulgaris extract on the isolated guinea-pig trachea: discrimination between drug and ethanol
effects. Planta Med 65:512–516
Mekkiou R, Touahar H, Dijoux-Franca MG, Mariotte AM, Benayache S, Benayache F (2005) A
new isoflavone from Genista saharae (Fabaceae). Biochem Syst Ecol 33:635–638
Meot-Duros L, Magné C (2009) Antioxidant activity and phenol content of Crithmum maritimum
L. leaves. Plant Physiol Biochem 47:37–41
Merzouki A, Derfoufi A, El-Aallau A, Molero-mesa J (1997) Wild medicinal plants used by local
Bouhmed population (Morocco). Fitoterapia 68:444–460
Mighri H, Akrout A, El-jeni H, Zaidi S, Tomi F, Casanova J, Neffati M (2010a) Composition and
intraspecific chemical variability of the essential oil from Artemisia herba alba growing wild
in a Tunisian arid zone. Chem Biodivers 7:2709–2717
Mighri H, Hajlaoui H, Akrout A, Najjaa H, Neffati M (2010b) Antimicrobial and antioxidant
activities of Artemisia herba-alba essential oil cultivated in Tunisian arid zone. C R Chim
13:380–386
Milos M, Radonic A (2000) Gas chromatography mass spectral analysis of free and glycosidically
bound volatile compounds from Juniperus oxycedrus L. growing wild in Croatia. Food Chem
68:333–338
Miresmailli S, Bradbury R, Isman MB (2006) Comparative toxicity of Rosmarinus officinalis L.
essential oil and blends of its major constituents against Tetranychus urticae Koch (Acari:
Tetranychidae) on two different host plants. Pest Manag Sci 62:366–371
Mittler R, Merquiol E, Hallak-Herr E, Rachmilevitch S, Kaplan A, Cohen M (2001) Living under
a “dormant” canopy: a molecular acclimation mechanism of the desert plant Retama raetam.
Plant J 25:407–416
Mizrahi I, Juarez MA, Bandoni A (1991) The essential oils of Rosmarinus officinalis growing in
Argentina. J Esent Oil Res 3:11–15
Mkaddem MG, Romdhane M, Ibrahim H, Ennajar M, Lebrihi A, Mathieu F, Bouajila J (2011)
Essential oil of Thymus capitatus Hoff. et Link. from Matmata Tunisia: gas chromatogra-
phy–mass spectrometry analysis and antimicrobial and antioxidant activities. J Med Food
13:1500–1504
Moghtader M, Afzali D (2009) Study of the antimicrobial properties of essential oil of rosemary.
American-Eurasian J Agr Envriron Sci 5(3):393–397
Morales R (1996) Studies on the genus Thymus L. Lamiales. Newsletter 4:6–8

Morales R (1986) Taxonom´ıa de los géneros Thymus (excluida la sección Serpyllum) y Thymbra
en la Pen ´ınsula Ibérica. Ruizia, 3,Monograf´ıas Del Real Jard´ın Botánico, Madrid
Munir C, Zaidi MI, Ahmad N, Rahman AU (1993) An easy rapid metal mediated method of isola-
tion of harmine and harmaline from Peganum harmala. Fitoterapia 1:73–76
Munoz-Reinoso JC (2004) Diversity of maritime juniper woodlands. Forest Ecol Manag
192:267–276
Murray RDH, Mtndez J, Brown SA (1982) The natural coumarins: occurrence, chemistry and
biochemistry, Wiley, Chichester
Nabli A (1989) Essai de synthèse sur la végétation et la phyto-écologie tunisienne. Tunis Imp. Off.
Rep, Tunisia, p 186
Nabli MA (1995) Essai de synthèse sur la végétation et la phyto-écologie tunisiennes. II et III. Le
milieu physique et la végétation. Ecologie végétale appliquée, No. 5, 6 I.O.R., Tunisia
Nadkarni AK (1954) Peganum harmala Linn. Indian Materia Medica, 3rd Edition, vol 1. G. R.
Bhatkal and Co., Bombay Popular Book Depot, Bombay, pp 927–929, 1319 pp
Najjaa H, Neffati M, Zouari S, Emmar E (2007) Essential oil composition and antibacterial activ-
ity of different extracts of Allium roseum L., a North African endemic species. CR Chimie
10:820–826
Najjaa H, Zouari S, Ammar E, Neffati M (2010) Phytochemical screening and antibacterial proper-
ties of Allium roseum L. a wild edible species in North Africa. J Food Biochem 35:699–714
Najjaa H, Zouari S, Arnault I, Auger J, Ammar E, Neffati M (2011a) Différences et similitudes
des métabolites secondaires chez deux espèces du genre Allium, Allium roseum L. et Allium
ampeloprasum L. Acta Bot Gallica 158(1):111–123
Najjaa H, Zria K, Fattouch S, Ammar E, Neffati M (2011b) Antioxidant and antimicrobial activi-
ties of Allium roseum L. “Lazoul,” a wild edible endemic species in North Africa. Prop Sci
Food 14:371–380
Nawwar MAM, El Sissi HI, Barakat HH (1984) Flavonoid constituents of Ephedra alata.
Nawwar MAM, Barakat HH, Buddrus J, Linscheid M (1985) Alkaloidal, lignan and phenlic con-
stituents of Epherdra alata. Phytochemistry 24(4):818–819
Neffati A, Skandrani I, Sghaier MB, Bouhlel I, Kilani S, Ghedira K, Neffati M, Cherif I, Hammami
M, Chekir-Ghedira L (2008) Chemical composition, mutagenic and antimutagenic activities
of essential oils from (Tunisian) Artemisia campestris and Artemisia herba-alba. J Essent Oil
Res 20(5):471–477
Neffati A, Bouhlela I, Ben Sghaiera M, Boubakera J, Limema I, Kilani S, Skandrania I, Bhouria W,
Le Dauphinc J, Barillier D, Mosrati R, Chekir-Ghediraa L, Ghediraa K (2009) Antigenotoxic
and antioxidant activities of Pituranthos chloranthus essential oils. Environ Toxicol Pharmacol
27:187–194
Newall CA, Anderson LA, Phillipson JD (1996) Herbal medicines. The Pharmaceutical Press,
London, p 165
Nguyen DV, Takascova M, Jakubik T, Dang MN (2000) Antioxidant effect of thyme in rape-seed
oil. Biologia 55:277–281
Odek-Ogunde M, Rajab MS, Migwi GJ, Ndegwa JM (1993) Blood pressure responses to an extract
of Ajuga remota in experimentally hypertensive rats. Planta Med 59:573–574
O’Hearn E, Molliver ME (1993) Degeneration of purkinje cells in parasagittal zones of the cer-
ebellar vermis after ibogaine or harmaline. Neuroscience 55:303–310
Ojala T, Remes S, Pasi Haansuu P, Vuorela H, Hiltunen R, Haahtela K, Vuorela P (2000)
Antimicrobial activity of some coumarin containing herbal plants growing in Finland.
J Ethnopharmacol 73(1–2):299–305
Ojewole JAO (2008) Anticonvulsant effect of Rhus chirindensis (Baker F.) (Anacardiaceae) stem-
bark aqueous extract in mice. J Ethnopharmacol 117(1):130–135
Olchowik E, Sciepuk A, Mavlyanov S, Abdullajanova N, Zamaraeva M (2012) Antioxidant capaci-
ties of polyphenols from Sumac (Rhus typhina L.) leaves in protection of erythrocytes against
oxidative damage. Biomed Prev Nutr 2(2):99–105
Olesen C, Sorensen TLM, Nielsen RC, Moller JV, Nissen P (2004) Dephosphorylation of the cal-
cium pump coupled to counterion occlusion. Science 306:2251–2255
Oliva A, Lahoz E, Contillo R, Aliotta G (1999) Fungistatic activity of Ruta graveolens extract and
its allelochemicals. J Chem Ecol 25:519–526
Oliva A, Meepagala KM, Wedge DE, Harries D, Hale AL, Aliotta G, Duke SO (2003) Natural
fungicides from Ruta graveolens L. leaves, including a new Quinolone Alkaloid. J Agric Food
Chem 51(4):890–896
Ormancey X, Sisalli S, Coutiere P (2001) Formulation of essential oils in functional perfumery.
Parfums, Cosmetiques, Actualites 157:30–40
Ould El Hadj MD, Hadj-Mahammed M, Zabeirou H (2003) Place des plantes spontanées dans la
médicine traditionnelle de la région d’Ouargla (Sahara septentrional est). Courrier du Savoir
3:47–51
Oury P (1984) Encyclopedia of medicinal plants and flowers, vol IV. Eds Alliance, Paris. (in
French)
Owen SM, Harley P, Guenther A, Hewitt CN (2002) Light dependency of VOC emissions from
selected Mediterranean plant species. Atmos Environ 36(19):3147–3159
Ozcan M (2000) The use of yogurt as starter in rock sapphire (Crithmum maritimum L.) fermenta-
tion. Z Eur Food Res Technol 210:424–426
Ozcan M (2005) Mineral composition of different parts of Capparis ovata Desf. Growing wild in
Turkey. J Med Food 8:405–407
Ozcan M, Akgul A (1998) Influence of species harvest date and size on composition of capers
(Capparis spp.) flower buds. Nahrung 42:102–105
Ozcan MM, Pedro LG, Figueiredo AC, Barroso JG (2006) Constituents of the essential oil of sea
fennel (Crithmum maritimum L.) growing wild in Turkey. J Med Food 9(1):128–130
Ozenda P (1991) Flore et végétation du Sahara, CNRS éditions
Pandey RC, Pathak RA, Pathak RK (1990) Physico-chemical changes associated with growth and
development of fruits in ber (Zizyphus mauritiana Lamk). Indian J Hortic 47(3):286–290
Pareek OP (2002) Ber-ziziphus Mauritania. Available by International centre for underutilized
crops (online). Available: http://www.civil.soton.ac.uk/icuc/cd-icuc-ber-tamarind/content/er/
ber-book-html via the INTERNET. Accesed 6 Apr 2004
Park Y, Moon B, Yang H, Lee Y, Lee E, Lim Y (2007) Complete assignments of NMR data of 13
hydroxymethoxyflavones. Magn Reson Chem 45:1072–1075
Pathak S, Multani AS, Banerji P, Banerji P (2003) Ruta 6 selectively induces cell death in brain
cancer cells but proliferation in normal peripheral blood lymphocytes: a novel treatment for
human brain cancer. Int J Oncol 23:975–982
Pearson HHW (1929) Gnetales. Cambridge University Press, Cambridge
Perez-Alonso MJ, Valasco NA, Emin DM, Harmandar M, Esteben JL (1995) Composition of the
essential oils of Ocimum basilicum var. glabratum and Rosmarinus officinalis from Turkey.
J Esent Oil Res 7(1):73–75
Pérez-Olleros L, Garcia-Cuevas M, Ruiz-Roso B (1999a) Influence of pulp and natural carob fiber
on some aspects of nutritional utilization and lipidaemia. Food Sci Technol Int 5:425–430
Pérez-Olleros L, Garcia-Cuevas M, Ruiz-Roso B, Requejo A (1999b) Comparative study of natu-
ral carob fibre and psyllium husk in rats. Influence on Some Aspects of Nutritional Utilization
and Lipidaemia. J Sci Food Agric 79:173–178
Perfumi M, Valentini G, Bellomaria B, Biondi E (1999) J Essent Oil Res 11:223
Pignatti S (1982) Flora d’Italia. Edagricole, Bologna 1:636–644
Pino JA, Estraron M, Fuentes C (1998) Essential oil of Rosemary (Rosmarinus officinalis L.) from
Cuba. J Esent Oil Res 10(1):111–112
Pintore G, Usai M, Bradesi P, Juliano C, Boatto G, Tomi F, Chessa M, Cerri R, Casanova J (2002)
Chemical composition and antimicrobial activity of Rosmarinus officinalis L. oils from
Sardinia and Corsica. Flavour Fragr J 17:15–19
Piozzi F, Rodriguez B, Savona G (1987) Advances in the chemistry of the furanoditerpenoids from
Teucrium species. Heterocycles 25:807–841
Piozzi F, Bruno M, Rosselli S, Maggio A (2006) The diterpenoids of the genus Marrubium
(Lamiaceae). Nat Prod Commun 11(7):585–592
Pirzada AJ, Shaikh W, Kazi TG (2007) Isolation of essential elements and inhibition activity of
medicinal plant Rhazya stricta Dcne. against dermatophytic fungi. Pak J Agri Agril Eng Vet
Sci 23(1):34–38
Pistelli L, Fiumi C, Morelli I (2003) Flavonoids from Calicotom villosa. Fitoterapia 74:417–419
Pitarokili D, Couladis M, Petsikos-Panayotarou N, Tzakou O (2002) Composition and antifungal
activity on soil-borne pathogens of the essential oil of Salvia sclarea from Greece. J Agric
Food Chem 50:6688–6691
Pöllmann K, Gagel S, Elgamal MHA, Shaker KH, Seifert K (1997) Triterpenoid saponins from the
roots of Zygophyllum species. Phytochemistry 44:485–489
Polunin O, Huxley A (1972) Flowers of the Mediterranean. Publications of Chatto and Windos
Ltd, London
Pottier-Alapetite G (1979) Flore de la Tunisie. Angiosperme–Dicotylédones. Vol. I: Apétales–
Dialypétales. Ministère de l’Enseignement Supérieur et de la Recherche Scentifique et le
Ministère de l’Agriculture, Tunis, Tunisie
Pottier-Alapetite G (1981) Flore de la Tunisie. Angiosperme–Dicotylédones. Vol. II: Gamopétales.
Ministère de l’Enseignement Supérieur et de la Recherche Scentifique et le Ministère de
l’Agriculture, Tunis, Tunisie
Prajapati V, Tripathi AK, Aggarwal KK, Khanuja SPS (2005) Insecticidal, repellent and
oviposition-deterrent activity of selected essential oils against Anopheles stephensi, Aedes
aegypti and Culex quinquefasciatus. Bioresour Technol 96:1749–1757
Preethi KC, Kuttan G, Kuttan R (2006) Antitumor activity of Ruta graveolens extract. Asian Pac
J Cancer Prev 7:439–443
Price RA (1996) Systematics of the Gnetales: a review of morphological and molecular evidence.
Int J Plant Sci 157(Suppl. 6):S40–S49
Puzii AD, Vecherkin SS, Tribunskii MP, Romakhov VG (1980) Toxicity of the combined alkaloids
of harmala (Peganum harmala, Zygophyllaceae). Veterinariia 4:57–58
Quezel P, Santa S (1962) Nouvelle Flore de l′Algérie et des Régions Désertiques, Méridionales,
tome II. Edition CNRS, Paris
Raghav SK, Gupta B, Agrawal C, Goswami K, Das H (2006) Antiinflammatory effect of Ruta
graveolens L. in murine macrophage cells. J Ethnopharmacol 104:234–239
Rasekh HR, Khoshnood-Mansourkhani MJ, Kamalinejad M (2001) Hypolipidemic effects of
Teucrium polium in rats. Fitoterapia 72:937–939
Rasmussen U, Christensen SB, Sandberg F (1978) Thapsigargin and Thapsigargicin, two new
Histamine Liberators from Thapsia garganica L. Acta Pharm Suee 15:133
Rasmussen U, Christensen SB, Sandberg F (1981) Phytochemistry of the genus Thapsia. Planta
Med 43:336–341
Raul NC Jr, Lucinewton SM, Paulo TVR, Meireles MAA (2005) Supercritical fluid extraction
from rosemary (Rosmarinus officinalis): kinetic data, extract’s global yield, composition, and
antioxidant activity. J Supercrit Fluids 35:197–204
Rauter AP, Martins A, Lopes R, Ferreira J, Serralheiro LM, Araujo ME, Borges C, Justino J, Silva
FV, Goulart M, Thomas-Oates J, Rodrigues JA, Edwards E, Noronha JP, Pinto R, Mota-Filip
H (2009) Bioactivity studies and chemical profile of the antidiabetic plant Genista tenera.
Razzaghi-Abyaneh M, Shams-Ghahfarokhi M, Rezaee MB, Jaimand K, Alinezhad S, Saberi R,
Yoshinari T (2009) Chemical composition and antiaflatoxigenic activity of Carum carvi L.,
Thymus vulgaris and Citrus aurantifolia essential oils. Food Control 20:1018–1024
Reische DW, Lillard DA, Eintenmiller RR (1998) Antioxidants in food lipids. In: Ahoh CC, Min
DB (eds) Chemistry, nutrition and biotechnology. Marcel Dekker, New York, pp 423–448
Ren J (2001) The study on soil seed banks of desert vegetation and germination ecology of
Calligonum plants. Ph.D. thesis, Environment and Engineering Institute of Cold and Arid
Regions, The Chinese Academy of Sciences, P. R. China (in Chinese with English abstract)
Renault JH, Ghedira K, Thepenier P, Lavand C, Zeches-Hanrot M, Le Men-Olivier L (1997)

Dammarane saponins from Zizyphus lotus. Phytochemistry 44:1321–1327
Rezzi S, Cavaleiro C, Bighelli A, Salgueiro L, Hnio A, Da Cunha P, Casanova J (2001) Intraspecic
chemical variability of the leaf essential oil of Juniperus phoenicea subsp. turbinata from
Corsica. Biochem Syst Ecol 29:179–188
Rezzoug SA, Baghdadi MW, Isouka N, Boutekedjiret C, Allaf K (1998) Study of a new extraction
process: controlled instantaneous decompression. Application to the extraction of essential oil
from rosemary leaves. Flavour Fragr J 13(4):251–258
Richard H, Benjilali B, Banquour N, Baritaux O (1985) Etude de diverses huiles essentielles de
thym du Maroc. Lebensm-Wiss Technol 18:105–110
Rigano D, Formisano C, Basile A, Lavitola A, Senatore F, Rosselli S, Bruno M (2006) Antibacterial
activity of flavonoids and phenylpropanoids from Marrubium globosum ssp. libanoticum.
Rigano D, Venera C, Formisano C, Maldini MT, Piacente S, Bevilacqua J, Russo A, Senatore F
(2009) Genista sessilifolia DC. and Genista tinctoria L. inhibit UV light and nitric oxide –
induced DNA damage and human melanoma cell growth. Chem Biol Interact 180:211–219
Rima K, Hao Z, Wei C (2011) Antimicrobial and antioxidant activities of Chinese sumac (Rhus
typhina L.) fruit extract. Food Control 22(1):128–132
Rimbau V, Cerdan C, Vila R, Iglesias J (1999) Antiinflammatory activity of some extracts
from plants used in the traditional medicine of north-African countries (II). Phytother Res
13(2):128–132
Rivera D, Inocencio C, Obon C, Alcaraz F (2003) Review of food and medicinal uses of Capparis
L. subgenus Capparis (Capparidaceae). Econ Bot 57:515–534
Rodrigo M, Lazaro MJ, Alvarruiz A, Giner V (1992) Composition of capers (Capparis spinosa):
influence of cultivar, size and harvest date. J Food Sci 57:1152–1154
Romeo V, Ziino M, Giuffrida D, Condurso C, Verzera A (2007) Flavour profile of capers (Capparis
spinosa L.) from the Eolian archipelago by HS-SPME/GC-MS. Food Chem 101:1272–1278
Rota C, Carraminana JJ, Burillo J, Herrera A (2004) In vitro antimicrobial activity of essential oils
from aromatic plants against selected foodborne pathogens. J Food Prot 67:1252–1256
Rota MC, Herrera A, Martínez RM, Sotomayor JA, Jordán MJ (2008) Antimicrobial activity and
chemical composition of Thymus vulgaris, Thymus zygis and Thymus hyemalis essential oils.
Food Control 19(7):681–687
Rotblatt M, Ziment I (2002) Evidence-based herbal medicine. Philadelphia Hanley & Belfus
Ruberto G, Biondi D, Piattelli M (1991) Composition of the volatile oil of Crithmum maritimum
L. Flavour Fragr J 6(2):121–123
Ruberto G, Baratta MMT (2000) Antioxidant activity of selected essential oil components in two
lipid model systems. Food Chem 69:167–171
Saadaoui E, Khaldi A, Khouja ML, El-Gazzah M (2007) Etude de la variabilité morphologique du
câprier (Capparis spp.) en Tunisie. Revue des régions arides 2:523–527
Sacchetti G, Maietti S, Muzzoli M, Scaglianti M, Manfredini S, Radice M, Bruni R (2005)
Comparative evaluation of 11 essential oils of different origin as functional antioxidants, anti-
radicals and antimicrobials in foods. Food Chem 91:621–632
Safaei-Ghomi J, Ebrahimabadi AH, Djafari-Bidgoli Z, Batooli H (2009) GC/MS analysis and in
vitro antioxidant activity of essential oil and methanol extracts of Thymus caramanicus Jalas
and its main constituent carvacrol. Food Chem 115:1524–1528
Safir O, Fkih-Tetouani S (1998) Saponins from Zygophyllum gaetulum. J Nat Prod 61:130–134
Saha JC, Savini EC, Kasinathan S (1961) Indian J Med Rex 49:130
Said O, Khalil K, Fulder S, Azaizeh H (2002) J Ethnopharmacol 83:251
Saieed P, Reza RM, Abbas D, Seyyedali R, Aliasghar H (2006) Inhibitory effects of Ruta graveo-
lens L. extract on guinea pig liver aldehyde oxidase. Chem Pharm Bull 54:9–13
Sakakibara K, Nishiyama T, Sumikawa N, Kofuji R, Murata T, Hasebe M (2003) Involvement
of auxin and a homeodomain-leucine zipper I gene in rhizoid development of the moss
Physcomitrella patens. Development 130:4835–4846
Salido S, Altarejos J, Nogueras M, Shanchez A (2001) Chemical composition of essential oil of

Artemisia herba alba Ass. Ssp. valentine (Lam.) Marc. J Essent Oil Res 13:221–224
Salido S, Valenzuela LR, Altarejos J, Nogueras M, Sanchez A, Cano E (2004) Composition and
infraspecific variability of Artemisia herba-alba from southern Spain. Biochem Syst Ecol
32:265–277
Sawamura M (2000) Aroma and functional properties of Japanese yuzu (Citrus junos Tanaka)
essential oil. Aroma Res 1:14–19
Scarpato R, Paganucci L, Bertoli A, Fiore L, Pistelli L, Federico G (2008) Licoflavone C atten-
uates the genotoxicity of cancer drugs in human peripheral lymphocytes. Phytother Res
22:1650–1654
Schraudolf H (1989) Indole glucosinolates of Capparis spinosa. Phytochemistry 88:259–260
Senatore F, De Feo V (1994) Essential oil of a possible new chemotype of Crithmum maritimum
L. growing in Campania (southern Italy). Flavour Fragr J 9:305–307
Semmar N, Jay M, Chemli R (2001) Chemical diversification trends in Astragalus caprinus
(Leguminosae), based on the flavonoid pathway. Biochem Syst Ecol 29:727–738
Semmar N, Fenet B, Gluchoff-Fiasson K, Comte G, Jay M (2002) New flavonol tetraglycosides
from Astragalus caprinus. Chem Pharm Bull 50:981–984
Semmar N, Jay M, Farman M, Chemli R (2005) Chemotaxonomic analysis of Astragalus caprinus
(Fabaceae) based on the flavonic patterns. Biochem Syst Ecol 33:187–200
Sharififar F, Dehghn-Nudeh GH, Mitrajaldini M (2009) Major flavonoids with antioxidant activity
from Teucrium polium L. Food Chem 112:885–888
Sher H, Alyemeni MN (2010) Ethnobotanical and pharmaceutical evaluation of Capparis spinosa
L, validity of local folk and Unani system of medicine. J Med Plant Res 4(17):1751–1756
Sher H, Hussain F (2009) Ethnobotanical evaluation of some plant resources in Northern part of
Pakistan. Afr J Biotechnol 8(17):4066–4076
Sher H, Al-Yemeni MN, Sher H (2010a) Forest Resource utilization assessment for economic
development of rural community, Northern parts of Pakistan. J Med Plant Res 4(12):1197–1208
Sijelmassi A (1993) Les plantes médicinales du Maroc. Editions Le Fennec, Casablanca, 286 pp
Skidmore-Roth L (2001) Mosby’s handbook of herbs and natural supplements, 1st edn. Mosby,
New York, pp 741–744
Skima F, Kaaya A, Jaouhari JT, Lazrek HB, Jana M, El Amri H (1999) Hypoglycaemic activity of
Globularia alypum leaves in rats. Fitoterapia 70:382–389
Snoussi M, Hajlaoui H, Noumi E, Usai D, Sechi LA, Zanetti S, Bakhrouf A (2008) In vitro
anti-Vibrio spp. activity and chemical composition of some Tunisian aromatic plants. World
J Microbiol Biotechnol 24:3071–3076
Sobhani AM, Ebrahimi SA, Hoormand M, Rahbar N, Mahmoudian M (2002) Cytotoxicity of
Peganum harmala L. seeds extract and its relationship with contents of β-carboline alkaloids.
J Iran Univ Med Sci 8:432–438
Soliman KM, Badeaa RI (2002) Effect of oil extracted from some medicinal plants on different
mycotoxigenic fungi. Food Chem Toxicol 40:1669–1675
Somer G, Calışkan AC (2007) Selenium and trace element distribution in Astragalus plants:
developing a differential pulse polarographic method for their determination. Turk J Chem
31:411–422
Sommer A, Davidson FR (2002) Assessment and control of vitamin A deficiency: the annecy
accords. J Nutr 132:2845S–2850S
Spera D, Siciliano T, De Tommasi N, Braca A, Vessières A (2007) Antiproliferative cardenolides
from Periploca graeca. Planta Med 73:384–387
Stahl-Biskup E (1991) The chemical composition of Thymus oils: a review of the literature 1960–
1989. J Essent Oil Res 3:61–82
Stahl-Biskup E, Sáez F (2002) Thyme: the genus Thymus. Taylor and Francis, London
Stashenko EE, Acosta R, Martínez JR (2000) High-resolution gas-chromatographic analysis of
the secondary metabolites obtained by subcritical-fluid extraction from Colombian rue (Ruta
graveolens L.) J Biochem Biophys Methods 43(1–3):379–390
Stassi V, Verykokidou A, Loukis A (1996) The antimicrobial activity of the essential oils of four
Juniperus species growing wild in Greece. Flavour Fragr J 11:71–74
Stevanovits-Banyai E, Tulok MH, Hegedus A, Renner C, Varga IS (2003) Antioxidant effect of
various rosemary (Rosmarinus officinalis L.) clones. Acta Biol Szegediensis 47(1–4):111–113
Stevenson DW (1993) Ephedraceae. In: Flora of North America Editorial Committee (eds) Flora
of North America, vol 2. Oxford University Press, New York, pp , 428–434
Stratton SE, Lorden JF (1991) Effect of harmaline on cells of the inferior olive in the absence of
tremor: differential response of genetically dystonic and harmaline-tolerant rats. Neuroscience
41:543–549
Stulzer HK, Tagliari MP, Zampirolo JA, Cechinel-Filho V, Schlemper V (2006) Antioedematogenic
effect of marrubiin obtained from Marrubium vulgare. J Ethnopharmacol 108(3):379–392
Sushila R, Permender R, Dharmender R, Deepti R, Vikash K (2010) Phytochemical and pharma-
cological Potential of Kair (Capparis Decidua). Int J Phytomed 2:10–17
Syrov VN, Khushbaktova ZA (2001) Experimental study of pharmacotherapeutic effect of phyto-
ecdysteroids and nerobol in toxic liver damage. Eksp Klin Farmakol 64(4):56–58
Syrov VN, Nabiev AN, Sultanov MB (1986) Action of phytoecdysteroids on the bile-secretory
function of the normal liver and in experimental hepatitis. Farmakol Toksikol 49:100–103
Syrov VN, Nasyrova SS, Khushbaktova ZA (1997) The results of experimental study of phytoec-
dysteroids as erythropoiesis stimulators in laboratory animals. Eksp Klin Farmakol 60:41–44
Täckholm V (1974) Students’ flora of Egypt, 2nd edn. Cairo University, Cairo, p 888
Tahraoui A, El-Hilalya J, Israili ZH, Lyoussi B (2007) J Ethnopharmacol 110:105
Takahashi D, Miura K (1888) Mitt Med Fak Tokyo 1:255
Takeda Y, Yanagihara K, Masuda T, Otsuka H, Honda G, Takaishi Y, Sezik E, Yesilada E (2000)
Labdane diterpenoids from Marrubium globosum ssp. globosum. Chem Pharm Bull (Tokyo)
48(8):1234–1235
Taleb-Senouci D, Ghomari H, Krouf D, Bouderbala S, Prost J, Lacaille-Dubois MA, Bouchenak
M (2009) Antioxidant effect of Ajuga iva aqueous extract in streptozotocin-induced diabetic
rats. Phytomedicine 16:623–631
Tariq M, Ageel AM, Al-Yahya MA, Mossa JS, Al-Said MS (1989) Anti-inflammatory activity of
Teucrium polium. Int J Tissue React 11(4):185–188
Tengberg A, Chen D (1998) A comparative analysis of nebkhas in central Tunisia and northern
Burkina Faso. Geomorphology 22:181–192
Tepe B, Sokmen M, Akpulat HA, Daferera D, Polissiou M, Sokmen A (2005) Antioxidative activ-
ity of the essential oils of Thymus sipyleus subsp. sipyleus var. sipyleus and Thymus sipyleus
subsp. sipyleus var. rosulans. J Food Eng 66:447–454
Teyeb H, Zouari S, Douki W, Najjar MF, Neffati M (2010) Essentiel oils of leaves, flowers and
fruits of Atragalus gombiformis Pomel (Fabaceae). Acta Hort 853:ISHS
Teyeb H, Zouari S, Douki W, Najjar MF, Neffati M (2011) Variation in volatiles of Atragalus gom-
biformis Pomel. Verlag der Zeischrift fùr Naturforschung. Tùbingen:0939–5075
Teyeb H, Zanina N, Neffati M, Douki W, Najjar MF (2012) Cytotoxic and antibacterial activi-
ties of leaf extracts of A. gombiformis Pomel (Fabaceae) growing wild in Tunisia. Turk J Biol
36:53–58
Tharib SM, Gnan SO, Veitch GBA (1983) Antimicrobial activity of compounds from Artemisia
campestris. J Food Prot 46:185–187
Tigrine-Kordjani N, Meklati BY, Chemat F (2006a) Analysis by gas chromatography–mass
spectrometry of the essential oil of Zygophyllum album L., an aromatic and medicinal plant
growing in Algeria. Int J Aromather 6(3–4):187–191
Tigrine-Kordjani N, Meklati BY, Chemat F (2010) Contribution of microwave accelerated distil-
lation in the extraction of the essential oil of Zygophyllum album L. Phytochem Anal 22:1–9
Tlig T, Gorai M, Neffati M (2008) Germination responses of Diplotaxis harra to temperature and
salinity. Flora 203:421–428
Tlili N, Nasri N, Saadaoui E, Khaldi A, Triki S (2009) Carotenoid and tocopherol composition
of leaves, buds, and flowers of Capparis spinosa grown wild in Tunisia. J Agric Food Chem
57:5381–5385
Tlili N, Khalidi A, Triki S, Munné-Bosch S (2010) Phenolic compounds and vitamin antioxidants
of Caper (Capparis spinosa). Plant Foods Hum Nutr 65:260–265
Tomei PE, Cioni PL, Flamini G, Stefani A (1995) Evaluation of the chemical composition of the
essential oils of some Lamiaceae from Serrania de Ronda (Andalucia, Spain). J Esent Oil Res
7(3):279–282
Tosun F, Akyuz-Kizilay C, Tosun AU (2009) Flavonoïds and isoflavonoïds from Genista sessilifo-
lia DC. Growing in Turkey. Chem Nat Compd 45(1):83–84
Touil A, Rhouati S, Creche J (2006) Flavonoid glycosides from Pituranthos chloranthus. Chem
Nat Compd 42(1):104–105
Tregenza LA (1955) The Red Sea mountains of Egypt. Oxford University Press, London, pp viii, 247
Trovato A, Monforte MT, Forestieri AM, Pizzimenti F (2000) In vitro anti-mycotic activity of
some medicinal plants containing flavonoids. Boll Chim Farm 139(5):225–227
Tutin TG (1972) Flora Europea. University Press, Cambridge
Uphof JCT (1968) Dictionary of economic plants. Verlag von Cramer, Germany, p 290
Vahedi K, Domigo V, Amarenco P, Bousser MG (2000) Ischaemic stroke in a sportsman who
consumed MaHuang extract and creatine monohydrate for body building [Letter]. J Neurol
Neurosurg Psychiatry 2000:68112–68113
Verite P, Nacer A, Kabouche Z, Seguin E (2004) Composition of seeds and stems essential oil of
Pituranthos scoparius (Coss.& Dur.) Schinz. Flavour Fragr J 19:562–564
Vernin G, Merad O, Vernin GMF, Zamkotsian RM, Parkanyi C (1995) GC-MS analysis of
Artemisia herba-alba Asso essential oils from Algeria. Dev Food Sci 37A:147–205
Vidrich V, Michelozzi M (1993) Sulla resae compozione stagionale di olio essenziale di galbule e
foglie di Juniperus phoenicea L. Italia Forestale e Montana 48:133–140
Vourlioti-Arapi F, Michaelakis A, Evergetis E, Koliopoulos G, Haroutounian SA (2012) Essential
oils of indigenous in Greece six Juniperus taxa: Chemical composition and larvicidal activity
against the West Nile virus vector Culex pipiens. Parasitol Res 110:1829–1839
Wang RZ (2007) C4 plants in the deserts of China: occurrence of C4 photosynthesis and its mor-
phological functional types. Photosynthetica 45:167–171
Wang W, Wu N, Zu YG, Fu YJ (2008) Antioxidative activity of Rosmarinus officinalis L. essential
oil compared to its main components. Food Chem 108:1019–1022
Watt OM, Breyer-Brandwijk MG (1962) The medicinal and poisonus plants of Southern and
Eastern Africa. E and S Livingstone, Edinburgh, p 841
Wealth of India (1992) A dictionary of Indian raw material and industrial products, vol 3. CSIR,
New Delhi, pp 213–214
White LM, Gardner SF, Gurley BJ, Marx MA, Wang P-L, Estes M (1997) Pharmacokinetics and
cardiovascular effects of MaHuang (Ephedra sinica) in normotensive adults. J Clin Pharmacol
37:116–122
Wichtel M, Anton R (1999) Plantes Thérapeutiques. Tradition, pratique, officinale, science et
thérapeutique. Tec and Doc (Eds)/EM inter, Paris, p 341
Wolters B, Eilert U (1981) Antimicrobial substances in callus cultures of Ruta graveolens. Planta
Med 43:166–174
Wu T-S, Shi LS, Wang JJ, Iou SC, Chang HC, Chen YP, Kuo YH, Chang YL, Tenge CM (2003)
Cytotoxic and antiplatelet aggregation principles of Ruta Graveolens. J Chin Chem Soc
50(1):171–178
Yang J, Du Y (2003) Chemical modification, characterization and bioactivity of Chinese lacquer
polysaccharides from lac tree Rhus vernicifera against leukopenia induced by cyclophospha-
mide. Carbohydr Polym 52:405–410
Yangui T, Bouaziz M, Dhouib A, Sayadi S (2009) Potential use of Tunisian Pituranthos chloran-
thus essential oils as natural disinfectant. Lett Appl Microbiol 48:112–117
Yesilada E, Bedir E, Caliş I, Takaishi Y, Ohmoto Y (2005) Effects of triterpene saponins from
Astragalus species on in vitro cytokine release. J Ethnopharmacol 96(1–2):71–77
Yin G, Jeney G, Racz T, Xu P, Jun X, Jeney Z (2006) Effect of two Chinese herbs (Astragalus
radix and Scutellaria radix) on non-specific immune response of tilapia, Oreochromis niloti-
cus. Aquaculture 253:39–47
Youdim KA, Deans SG, Finlayson HJ (2002) The antioxidant properties of thyme (Thymus zygis
L.) essential oil: an inhibitor of lipid peroxidation and a free radical scavenger. J Essent Oil
Res 14:210–215
Yue-lan C, Xin L, Min Z (2010) Capparis spinosa protects against oxidative stress in systemic
sclerosis dermal fibroblasts. Arch Dermatol Res 302:349–355
Zaabat N, Hay AE, Michalet S, Darbour N, Bayet C, Skandrani I (2011) Antioxidant and antigeno-
toxic properties of compounds isolated from Marrubium deserti de Noe. Food Chem Toxicol
49(12):3328–3335
Zakarya D, Fkih-Tetouani S, Hajji F (1993) Chemical compositionantimicrobial activity relation-
ships of Eucalyptus essential oils. Plantes Med Phytother 26:319–331
Zambonelli A, D’aulerio AZ, Severi A, Benvenuti S, Maggi L, Bianchi A (2004) Chemical com-
position and fungicidal activity of commercial essential oils of Thymus vulgaris L. J Essent Oil
Res 16:69–74
Zaouali Y, Messaoud C, Ben Salah A, Boussaid M (2005) Oil compositon variability among popu-
lations in relationship with their ecological areas in Tunisia. Flavour Fragr J 20(5):512–520
Zargham R, Zargham H (2008) Tannin extracted from Sumac inhibits vascular smooth muscle cell
migration. McGill J Med 11:119–123
Zarre S (2000) Systematic revision of Astragalus sect. Adiaspastus, sect. Macrophyllium and sect.
Pterophorus. Englera 18:1–219
Zellagui MA, Rhouti S, Creche J, Toth G, Ahmed AA, Pare PW (2004) Antimicrobial activity of
the alkaloid extract of Genista microcephala: isolation and complete 1H and 13C. Chemical
shifts assignments of lupanine and S-balycotomine. Revista Latinoamer Quím 32(3):109–114
Zhang T, Tan DY (2009) An examination of the function of male flowers in an andromonoecious
shrub Capparis spinosa. J Integr Plant Biol 51(3):316–324
Zhao J, Li SP, Yang FQ, Li P, Wang YT (2006) Simultaneous determination of saponins and fatty
acids in Ziziphus jujuba (Suanzaoren) by high performance liquid chromatography-evaporative
light scattering detection and pressurized liquid extraction. J Chromatogr A 1108:188–194
Zhao Z, Liu M, Tu P (2008) Characterization of water soluble polysaccharides from organs of
Chinese Jujube (Ziziphus jujuba Mill. cv. Dongzao). Eur Food Res Technol 226:985–989
Zhu YP (1998) Chinese Materia Medica: chemistry, pharmacology and applications. Harwood
Academic, Amsterdam
Ziyyat A, Legssyer H, Mekhfi H, Dassouli A, Serhouchni M, Benjelloun W (1997) Phytotherapy of
hypertension and diabetes in oriental Morocco. J Ethnopharmacol 58:45–54
Zohary M (1972) Flora of Palaestina, vol 2. p 25
Zohary M (1973) The geobotanical foundations of the middle East, Fischer G (ed). Stuttgart, 738p
Zunft HJF, Lueder W, Harde A, Haber B, Graubaum HJ, Gruenwald J (2001) Carob pulp prepara-
tion for treatment of hypercholesterolemia. Adv Ther 18:230–236
Zunft HJF, Lueder W, Harde A, Haber B, Graubaum HJ, Koebnick C, Gruenwald J (2003) Carob
pulp preparation rich in insoluble fibre lowers total and LDL cholesterol in hypercholesterol-
emic patients. Eur J Nutr 42:235–242
Promising Indigenous and Endemic Medicinal
Plants from Mauritius
Mohamad Fawzi Mahomoodally
and Muhammad Zakariyyah Aumeeruddy
Abstract The Republic of Mauritius is part of Sub-Saharan Africa and one of the
three islands that constitute the Mascarene archipelago. Due to its volcanic origin,
the tropical island of Mauritius has a diverse flora and has been endorsed by inter-
national authorities as one of the Indian Ocean islands biodiversity hotspots. The
local flora bears its essence from the floristic heritage of the Mascarenes and harbor
a wide array of endemic or highly specialized species that has evolved as a result of
the variation in topography, climate and the geographical isolation of the islands.
Indeed, a major portion of this flora is exploited for its medicinal properties by the
local people to treat and/or manage common ailments. Nonetheless, there is still a
dearth of scientific validation and compilation of some indigenous plants in com-
mon use. The present chapter endeavors to highlight the botanical description, eth-
nopharmacological uses, and the main therapeutic benefits of common indigenous
and endemic medicinal plants of Mauritius. Special emphasis has been geared
towards recent in vitro and in vivo data which tend to support ethnopharmacological
uses of these medicinal plants in traditional medicine of Mauritius.
Keywords Mauritius • Indian Ocean • Medicinal plants • Traditional medicines •

Ethnopharmacology
M.F. Mahomoodally (*)

Department of Health Sciences, Faculty of Science, University of Mauritius,
230 Réduit, Mauritius
M.Z. Aumeeruddy
Department of Agricultural and Food Science, Faculty of Agriculture, University of
Mauritius, 230 Réduit, Mauritius

DOI 10.1007/978-94-024-1120-1_9
232 M.F. Mahomoodally and M.Z. Aumeeruddy
1 Introduction
The tropical island of Mauritius forms part of the African continent and lies in the
southern hemisphere in the middle of the Indian Ocean with latitude and longitude
20.1625°S, 58.2903°E. The Republic of Mauritius is composed of the several
islands including of Mauritius, Rodrigues, Agalega, St. Brandon and a number of
outlying smaller islands, all located in the south of the Indian Ocean. Mauritius is
the principal island some 800 km from the south east of Madagascar. Mauritius has
an area of 1865 km2. Mauritius enjoys a mild tropical maritime climate throughout
the year, characterized by a warm humid summer extending between November and
April and a relatively cool dry winter between June and September (Sreekeeson and
Mahomoodally 2014).
As at 01st July 2014 the population of the island stood at 1.219.265 individuals
with 603.473 males and 615.792 females (CSO 2014). About half of the population
is concentrated in the urban areas which lie along the axis from the capital Port-
Louis to the city of Curepipe. The standards of health, nutrition and education are
high compared to other countries in Africa. The adult literacy rate is 83% and
according to the most recent WHO bulletin published in 2013, the life expectancy at
birth is about 70.4 for males and 78 for females (World Health Rankings 2014).
The population comprises of Indo-Mauritians, people of mixed European and
African origin and Sino-Mauritians. The main ethnic groups are the Bhojpuri speak-
ing Hindus, constituting 40.2% of the total population. The Tamils are the second
largest ethnic community (13.9%), while Telugus (5.6%) and Marathis (4%) repre-
sent smaller minorities within the overall Hindu population. The Hindus have a
common language (Bhojpuri), the same regional origin (Uttar Pradesh and Bihar),
religious practices, and rituals (Hollup 1994, 2012).
Since the past years, Mauritius economy has switched from sugar and textiles,
into financial services and information and communication technology, as well as
providing a seafood hub for the region and becoming a destination for medical tour-
ism and a centre for academic excellence (Joseph and Troester 2012).
Mauritius has a rich heritage of indigenous and endemic plants and possesses 58
families of plant species consisting of both indigenous and endemic plants. A total
of 711 indigenous plant species has been recorded in Mauritius, including 246
endemic plant species (MOAIFS 2012). Mauritius is characterized by considerable
endemism rate and species diversity.
Nonetheless, the surge in population and economic growth has led to loss, deg-
radation and decline of ecosystems, species and genetic diversity. Economic devel-
opment has stimulated forest clearance for settlement, industries and agriculture as
well as intrusion on environmentally sensitive areas, including mountains and for-
ested areas, hence resulting in biodiversity erosion. Natural disasters such as
cyclones and droughts are significant threats to native biodiversity as it is highly
fragmented and the populations are small, resulting in diminished adaptability to
weather variations. Biodiversity consequently remains one of the top national pri-
orities of the local authority. According to the National Biodiversity Strategy and
Promising Indigenous and Endemic Medicinal Plants from Mauritius 233
Action Plan, it is estimated that Rs. 200 million is spent annually, in addition to
external funding, on the conservation of forests and terrestrial biodiversity. A num-
ber of policies and strategies have been adopted by Government for conservation
and sustainable use of biodiversity. These comprise of the National Environment
Policy (2007), National Biodiversity Strategy and Action Plan (2006–2015),
National Invasive Alien Species Strategy and Action Plan (2010), Non-Sugar Sector
Strategic Plan (2003–2007), Strategic Options in Crop and Livestock Sector102
(2007–2015) and Study on Environmentally Sensitive Areas.
2 Medicinal Plants of Mauritius
Mauritius is famous for its floricultural diversity and for the panoply of traditions
practiced across the island by various ethnic groups. Moreover, the island is
renowned for the extraordinary richness of its flora, which harbors an array of
medicinal plant species. Traditional medicine is omnipresent in the Mauritian com-
munity whereby Mauritians still use traditional medicine including mainly medici-
nal plants for the treatment and/or management of various ailments. Indeed, the
local population has a long-standing tradition in the use of herbal medicine. Many
indigenous and endemic plant species of Mauritius have been used in folkloric med-
icine to treat various ailments of man including chronic diseases communicable and
non-communicable diseases.
Available reports tend to highlight that indigenous and native folk-medicinal
plant conservation and study is of uttermost importance because such plants are
fully adapted to local environments (e.g. tropical climates, altitudes, humidity and
temperature) compared to exotic species. Pharmacologically active compounds and
phytochemicals isolated from endemic and indigenous plants used in folk medicine
in Mauritius have been the areas of interest (Mahomoodally 2012a, b, c; Narod et al.
2004). Currently, several kinds of extracts from various exotic, endemic and indig-
enous plants are sold as decoctions or “tisanes” in several markets across Mauritius
to treat minor ailments (Mahomoodally 2012a, b, c). This native herbal folk medici-
nal practice forms an essential part of the heritage of the local pharmacopoeia of
Mauritius. Nonetheless, there is still a dearth of scientific validation and compila-
tion of some indigenous plants in common use.
The present chapter endeavours to highlight the botanical description, ethno-
pharmacological uses, and main therapeutic benefits of common indigenous and
endemic medicinal plants of Mauritius. Special emphasis has been geared towards
recent in vitro and in vivo data which tend to support ethnopharmacological uses in
the traditional medicine of Mauritius.
calypha integrifolia Willd. subsp. integrifolia var. integrifolia

A
(Euphorbiaceae)
Acalypha integrifolia is an indigenous species of the Mascarenes islands. It is com-

monly known as ‘bois queue de rat’, ‘bois de crève’ and ‘bois de Charles’ (Gurib-
Fakim and Gueho 1996). In Réunion Island and Mauritius, decoction of the leaves
is consumed as astringent and purgative and is used to eliminate intestinal worms.
A bath in the leaf decoction is taken to treat skin infections. The leaves, stems and
roots have been reported to contain saponins, tannins, sterols, terpenes and traces of
alkaloids (Gurib-Fakim and Gueho 1996; Seebaluck et al. 2015).
Leaf decoction, dichloromethane (DCM), ethyl acetate (EtOAc) and methanol
(MeOH) extract were found active against ATCC bacterial strains of Escherichia
coli, Pseudomonas aeruginosa and Staphylococcus aureus. MeOH extract also
inhibited the growth of clinical isolates namely Escherichia coli, Staphylococcus
aureus, Acinetobacter baumannii, Klebsiella pneumonia, Streptococcus group B,
Pseudomonas aeruginosa, Enterococcus faecalis, Proteus vulgaris and Candida
albicans. Hexane, DCM and EtOAc extracts showed antifungal activity against
Candida albicans, ATCC 10231 and Candida tropicalis, ATCC 750 (Unpublished
data).
EtOAc and MeOH extracts of the plant scavenged DPPH radical, ABTS cation
radical and hypochlorus acid. MeOH extract showed potential antioxidant and
reducing activity. Phytochemical screening of MeOH extract revealed the presence
of tannins, polyphenol, terpenes, saponins, anthocyanins, coumarins, flavonoids and
sugars (Unpublished data).
Antidesma madagascariense Lam. (Euphorbiaceae)
Antidesma madagascariense is native to the Mascarene Islands where it occurs in

Mauritius and Reunion Islands. Commonly known as ‘Bois bigaignon bâtard’ and
‘Bois bigayon’ in Mauritius, it is widely distributed in humid forests but can also be
seen in Reunion Island in dense thickets of medium altitude (100–1600 m). Other
familiar vernacular names include ‘Bois de cabri’, ‘Bois de cabri blanc’, ‘Bois de
gaulette blanc’ and ‘Bois d’oiseaux’ in Reunion island (Centre de cooperation inter-
national en recherché agronomique pour le développement 2006). The genus con-
sists of about 170 species growing in tropical and warm habitats. It is probably also
present in Madagascar.
This plant is common in humid forests in Mauritius and shows ample variations
in the texture and shape of the leaves which can be coriaceous to papery, narrowly
elliptic to broadly oval and the domatia very visible or missing in some cases. This
plant is characterised by the ample variations in the size, texture and shape of the
leaves which can be coriaceous to papery, often oval to elliptic, 4–10 cm long and
3–5 cm wide, the margins entirely or slightly sinuous. The presence of domatia is
very visible in the axils of primary nerves. This tree bears minute greenish or red
flowers of different sexes (Hoffman 1999; Kuete 2013). Often abundant in clusters
of small berries, the ovoid dark red fruits which turn into shiny purple black when
ripe, are more or less flattened and 6–7 mm long (Hoffman 1999; Centre de coop-
eration international en recherche agronomique pour le développement 2006; Jolly
et al. 2006).
A. madagascariense is very famous and widely used in folkloric medicine among
the local population of the Mascarene Islands for the treatment and management of
various ailments. In Mauritius, a decoction of the leaves and bark of Antidesma
madagascariensis is used as a diuretic, against fever, diabetes and as an astringent.
A decoction of the leaves is used to treat skin infections as well as rheumatism. Teas
are used against dysentery and albumin in urine. To treat jaundice a mixture of
Aphloia theiformis, Toddalia asiatica and Antidesma madagascariensis is used
(Narod et al. 2004; Gurib-Fakim 2011). The leaves and bark decoctions are diuretic
and are also used against fever, and for their astringent properties. The leaf tea is
used to treat dysentery. A bath in the leaf decoction is used against skin infections.
Along with other plants, it is reported as effective against jaundice. The decoction
obtained after boiling 10 leaves of A. madagascariense in 1 liter of water can be
consumed regularly for the treatment of albumin in urine (Kuete 2013). Furthermore,
the leaves and barks have been reported to possess diuretic, astringent as well as
febrifuge properties and also used diabetes management (Kuete 2013). A bath in the
leaf decoction has been reported to alleviate skin infections, rheumatic and body
aches. The leaves of A. madagascariense mixed with those of Aphloia theiformis
(Flacourtiaceae) and Toddalia asiatica (Rutaceae) are used to treat jaundice
(Hoffman 1999). Interestingly, A. madagascariense can be used to treat edema in
pregnancy and can also be employed in the case of stroke depending on the type of
decoction which is prepared (Kuete 2013).
Preliminary phytochemical screening of the leaves of A. madagascariense indi-
cates the presence of phenols, tannins, alkaloids, flavonoids, cyanogenetic hetero-
sides as well as leucoanthocyanins, sterols and saponins (Hoffman 1999; Narod
et al. 2004; Picot et al. 2013). Interestingly, A. madagascariense has also been found
to contain triterpenes and hydrolysable tannins, carpusin, and a dimer – antidesmin,
a common constituent also characterised in other Antidesma species (Mahomoodally
et al. 2006; Rangasamy et al. 2007). The bacteriostatic and bactericidal properties
of A. madagascariense, validated from the local folk medicine, can be attributed to
the presence of tannins in the plant. Furthermore, the aqueous and methanol extracts
of the leaves demonstrated their molluscicidal properties against species of
Biomphalaria and Bulinus and antifungal properties against Cladosporium cuc-
umerinum (Hoffman 1999; Kuete 2013). Different fractions of the leaves and stems
(water, methanol, chloroform and hexane) of A. madagascariense were previously
reported to have significant inhibitory effects on Gram-positive bacteria
Staphylococcus aureus, Gram-negative bacteria Pseudomonas aeruginosa and the
fungus Aspergillus niger showing their potent antimicrobial activities (Tchinda
et al. 2006; Gurib-Fakim et al. 2005). The ability of the methanol stem extracts of
A. madagascariense to exhibit contractile properties on rat ileal smooth muscles
coupled with the recent antioxidant and antimicrobial findings on A. madagas-

cariense validate its ethnobotanical use in the effective treatment of dysentery
(Hoffman 1999; Tchinda et al. 2006; Narod et al. 2004).
The different crude extracts of A. madagascariense exhibited potent antimicro-
bial activity which was found to increase with increasing polarity. The methanol
leaves extracts of A. madagascariense had potent inhibitory effects against
Enterococcus faecalis (minimum inhibitory concentration [MIC] = 60 μg/ml),
Staphyloccocus aureus (MIC = 500 μg/ml), Methicillin-resistant Staphylococcus
aureus (MRSA) (MIC = 250 μg/ml) and Candida albicans (MIC = 500 μg/ml)
(Narod et al. 2004).
We previously studied the antioxidant propensity of different crude extracts and
fractions of leaves of A. madagascariense using standard in vitro assays. The IC50
values ranged from 3.94 to 87.05 μg/ml, 3.18 to 13.26 μg/ml and 6.29 to 25.24 μ g/
ml for the 2,2-diphenyl-1-picrylhydrazine (DPPH), superoxide (SO) and nitric
oxide (NO) radical scavenging assays respectively. On the other hand, the n-butanol
extracts of A. madagascariense proved to be the most potent antioxidant against for
DPPH (% radical scavenging potential [RSP] = 93.68 ± 8.69%) and NO (% RSP =
65.56 ± 7.56%) assays, ethyl acetate extracts had a high percentage RSP (99.53 ±
7.53%) in SO assay (Mahomoodally et al. 2015). Likewise, a concentration of 0.5
mg/ml of methanol A. madagascariense leaves extracts was able to scavenge 70.6 ±
2.2% and 64.5 ± 1.8% of hydroxyl and hypochlorous acid radicals correspondingly,
thus validating the antioxidant properties of A. madagascariens (Narod et al. 2004).
Antiglycation activities were confirmed in the ethylacetate (96.63 ± 10.36%),
methanol (86.35 ± 5.65) and n-butanol (84.65 ± 6.35%) fractions of A. madagas-
cariense and this was comparable to the anti-glycation drug aminoguanidine.
Nevertheless, A. madagascariense extracts were found to have no activity against
mitochondrial respiration in a MTT cytotoxicity assay (P > 0.05) (Mahomoodally
et al. 2015). The efficacy of A. madagascariense extracts in managing diabetes was
assessed through the inhibition of key carbohydrate hydrolysing enzymes. All the
extracts exhibited variable inhibitory effects on α-amylase activity with ethylacetate
fraction having the best inhibitory effect (IC50 = 61.52 ± 11.09 μg/ml) which was
lower than acarbose (IC50 = 75.86 ± 8.16 μg/ml). Moreover, active fractions of A.
madagascariense were found to inhibit significantly amylase activity in mouse
plasma from 7.80% to 49.37%. α-glucosidase activity was significantly inhibited by
A. madagascariense extracts with IC50 values ranging from 19.70 ± 2.87 μg/ml to
44.92 ± 5.67 μg/ml which was comparable to the drug 1-deoxynojirimycin.
The repressive capacity of A. madagascariense extracts to increase blood glu-
cose concentration in mice was investigated by in vivo studies in glycogen-loaded
mice. Ethylacetate fraction was found to be more potent with glucose-lowering
properties (−59.4%) comparable to acarbose (−55.1%) (Picot et al. 2013). Moreover,
in vivo studies of A. madagascariense on rat everted intestinal sacs indicated that
aqueous extract A. madagascariense significantly enhanced the uptake of D-glucose
and fluid transport. It was also noted that a concentration of above 0.375 mg/ml of
the extract was needed to enhance mucosal disappearance, gut wall content and
serosal appearance of fluid. However, L-tyrosine and K+ transport was not signifi-
cantly enhanced to the contrary of Na+. Therefore, the ability of A. madagascariense
extracts to promote the transport of glucose, fluid and Na+ across rat everted intesti-
nal sacs might be attributed to the presence of bioactive phytochemicals, for
instance, flavonoids, alkaloids, leucoanthocyanins, phenols and saponins, in A.
madagascariense leaves which have possibly interact with the Na+/glucose co-
transporter in the enterocytes (Mahomoodally et al. 2012a, b, c).
The in vitro immunomodulatory property of A. madagascariense showed that
extracts were able to modulate significantly the immune response of phagocytes and
monocytes at different steps. At a concentration of 100 μg/ml, the inhibitory activity
of crude methanol A. madagascariense extracts on whole blood phagocytes for
reactive oxygen species (ROS) production was 94.2%. It was also suggested that A.
madagascariense directly inhibited a final common biochemical target such as
NADPH oxidase enzyme or scavenge ROS since it did not affect a specific trans-
ductional pathway.
Aphloia theiformis Benn. (Flacourtiaceae)
Aphloia theiformis is indigenous to Madagascar, the Comoros, the Mascarenes, the

Seychelles and tropical Africa, the species is still common in Mauritius and Reunion
Island. It has become extremely rare or is already extinct on Rodrigues. Common in
remnants of the native forest in the humid upland zone of Mauritius. In Comoros, A.
theiformis is known locally as the “Mfandrabo”, the leaves of which are widely used
alone or in combination for the preparation of tea. Previously included in the
Flacourtiaceae family, Aphloia theiformis is currently the only representative of the
Aphloiaceae family (Danthu et al. 2010). Aphloia theiformisis known to have high
morphological variations due to ecological factors.The synonyms are; Aphloia mau-
ritiana Baker, A. integrifolia (Vahl.) Benn., Lightfootia theiformis Vahl., Ludia het-
erophylla auct. Non Lam.
In Comoros, the A. theiformis leaves areused for treating dizziness and leucor-
rhoea. In Mauritius, they are also used as a febrifuge. In Madagascar, the bark of this
species is used as emetic; its young leavesare demonstrated effective against haema-
turia and the oldones against rheumatisms. In terms of chemical composition, the
literature reports the presence of saponins andxanthone in A. theiformis leaves
(Hsoidrou et al. 2014).
A tea made from the leaves of A. theiformis withthe bark of Terminalia bentzoe
has been reported to treat dysentery. The leaf decoction is used for fever (Adjanohoun
et al. 1983) and a diuretic (Sussman 1980; Wong Ting Fook 1980). It also helps
against rheumatism, ulcers, jaundice and gastrointestinal infections. Daruty (1886)
reports on the use of this plant as an emetic. The leaves, mixed with the bark of
Erythroxylum laurifolium and the roots of Cocos nucifera help against fever. In the
Comoros, a leaf decoction is used for stomach pains; in the Seychelles, a root and
leaf decoction helps against impetigo. Mixed withthose of Cinnamomum camphora
or those of Piper nigrum, a refreshing drink is obtained. A decoction of the leaves

mixed with those of Dracaena reflexa and those of Centella asiaticais given to
teething children. The root decoction is used to wash skin infections, while a leaf
poultice is applied to nail infection. A decoction of the root is reported to be a good
purgative. In Madagascar, Boiteau-Allorge (1999) reports that an infusion of the
leaves is diuretic, used against malarial fevers, haematuria, and seems to protect the
red blood corpuscles against lysis in vitro. In has also been reported that bile-related
problems are treated and/or managed by taking an oral infusion prepared from the
following mixture of a panoply of plants: leaves of A. theiformis, maize stigma,
pumpkin leaves, leaves of Cyperus sp., and plants of Euphorbia hirta.
The main constituent of A. theiformisis mangiferin, also known as aphloiol and
whose structure has been established as being C-glucoside of
1,3,6,7-tetrahydroxyxanthone (Paris and Etchepare 1964). Preliminary screenings
of the leaf extract showed the presenceof saponins and tannins (Smadja and Vera
1991). Lavergne (1990) reported on the composition of the leaves and stems: they
were found to contain phenols, saponins, terpenes, triterpenes, flavones, flavonoids
and traces of tannins. The plant growing in Madagascar is reported to be rich in
leucoanthocyanins, flavonoids, tannins, steroids, 2-desoxy-2 sugars, saponins and
traces of polyphenols (Gurib-Fakim and Guého 2000). The methanolic extract of
the leaves from Mauritius is reported to contain three triterpenes, namely tormenti-
cacid glucoside ester, 23-hydroxytormenticacid glucoside ester and
6β-hydroxytormentic acid (Gopalsamy et al. 1988). Previous works have reported
the presence of aphloiol, a tetrahydroxyflavone (Pernet 1957).
The anti-infective properties of A. theiformis were studied against different
strains of pathogenic bacteria and fungi, namely Salmonella enteritidis, Enterobacter
cloacae, and Bacillus subtilis, which were all found to be susceptible to the crude
methanolic extract (Rangasamy et al. 2007). The methanolic extracts of the leaves
kill the snail Biomphalaria glabra at a concentration of 400 ppm. This snail is a
vector for Schistosoma mansoni, the latter being responsible for the transmission of
the disease bilharzia (Gopalsamy et al. 1988).
Recently, Hsoidrou et al. 2014 investigated the immunomodulatory potential of
the phenolic fraction of A. theiformis leaves and their anti-inflammatory potent.
Phenolic fraction from the leaves of A. theiformis was obtained by methanol extract,
and recovered by an aqueous solution of 5% HCl, then filtered. We investigated the
potential immunomodulatory effect in rat, by flow cytometer. Anti-inflammatory
activity was also investigated in carrageenan-induced paw oedema rat model.
Results demonstrated an immunosuppressive effect on lymphocyte for all doses
administered. However, we noted an interesting immunostimulant effect on mono-
cytes and granulocytes number at high doses. Then, it was noted an important anti-
inflammatory-like effect for these doses in vivo. This work suggests the eventual use
of this plant in the treatment of some inflammatory symptoms and immune
dysfunctions.
Elaeodendron orientale Jacq. (Celastraceae)
Elaeodendron orientale Jacq. also known as Cassine orientalis Jacq. Kuntze.

(Patrimoine Réunion 2015) is an endemic plant of Madagascar and the Mascarene
Islands (Osorio et al. 2014). E.orientale belongs to the Celastraceae family and is
commonly known as ‘Bois d’olive’ and ‘Bois rouge’ in Mauritius and Réunion
island respectively (Patrimoine Réunion 2015). The bark and leaves of E. orientale
have been used in traditional medicine to treat various ailments namely, skin infec-
tions, veneral disease, scorpion fish poisoning (Osorio et al. 2014; RIS 2011), uri-
nary infections, ‘tambave’ (RIS 2011), hypertension (Neergheen et al. 2005) and
fish allergies (Gurib-Fakim et al. 2005). Traditionally, the bark is used for treating
venereal diseases, skin infections, and scorpion fish poisoning. The leaves have
astringent and emetic properties and are used to treat hypertension, and when mixed
with those of Kalanchoe pinnata (Crassulaceae), which produce bufadienolides,
can be used against fish allergies. Additionally, the leaves of E. orientale were
reported to possess astringent and emetic properties (Osorio et al. 2014).
Recently, it was reported that cardenolide glycosides isolated from the leaves and
fruits of E. orientale showed growth inhibition activity comparable to or higher than
digoxin and digitoxigenin against cancer cell lines namely, carcinoma of the cervix,
lung carcinoma and breast adenocarcinoma (Osorio et al. 2014). A new series of
cardenolides isolated from E. orientale have been evaluated as anticancer agents.
All the assayed cardenolide glycosides showed a potent growth inhibition activity
against human tumour and leukaemia cell lines, and four of showed an effect com-
parable to the reference agents, digoxin and digitoxigenin. In addition, and taking
into account the narrow security range of the GCs used in clinic, the authors pro-
posed that these new compounds show similar SI to reference drugs on various
cancer cell lines, thus increasing their potential for future study (Osorio et al. 2014).
Furthermore, the crude methanolic extract of E. orientale leaves was reported to
possess α-amylase (IC50 value 1745.58 ± 31.66 μg/ml compared to acarbose
1100.06 ± 0.03 μg/ml) and α-glucosidase (1.75 ± 0.26 μg/ml compared to acarbose
5115.73 ± 3.91 μg/ml) inhibitory properties. The extract exhibited an uncompetitive
type of inhibition against both enzymes (Picot et al. 2014). Catechin, procyanidin
dimers, kaempferol, myricetin and quercetin were found to be present in E. orien-
tale (Neergheen et al. 2005). Further scientific investigation of the prophylaxis
properties of E. orientale is crucial for the validation of ethnopharmacological data.
Erythroxylum laurifolium Lam. (Erythroxylaceae)
Erythroxylum laurifolium Lam. also known as Erythroxylum sideroxyloides Lam. is

an endemic plant of Mauritius and Réunion Island (Tisanes Indigènes 2014).
E.laurifolium belongs to the Erythroxylaceae family and is commonly known as
‘Bois de ronde’ and ‘Bois de rongue’ in Mauritius and Réunion Island respectively
(Ministry of Agriculture and Natural resources 1996; Tisanes Indigènes 2014).

E.laurifolium has been used in the Mauritian traditional medicinal system to treat
diabetes (Mootoosamy and Mahomoodally 2014; Tisanes Indigènes 2014), renal
stones (Richards 2012), nephritic spasm (Ministry of Agriculture and Natural
Resources 1996), fever, throat infections and as diuretic (Neergheen et al. 2005).
According to Lavergne (1990) the old medicinal use of E. laurifolium was as a
diuretic, an astringent and a remedy to treat renal colic, gravel, croup and inflamma-
tion of the throat. Most herbalists today use it as a remedy to treat renal colic,
against kidney disorders, kidney and urinary stones and as a diuretic (Lavergne
1990). E. laurifolium was reported to be an herbal ingredient of an herbal tea used
for the treatment of dysentery and kidney problems and composed of 15 cm of the
bark of E. laurifolium Lam., 10 leaves of Antidesma madagascariense Lam., a
handful of roots of Coix lacryma-jobi, 15 leaves of Aphloia theiformis, 0.5 cm of the
root of Rhizophora mucronata, 3 whole plants of Bidens pilosa and 10 leaves of
Piper species. The mixture was boiled in 1.5 l of water and was allowed to reduce
to 1 l (Narod et al. 2004).
Ethanolic extract (99%) of E. laurifolium leaves showed angiotensin converting
enzyme inhibitory activity. The activity was found to be due to proanthocyanidins
or condensed tannins and the flavonoids quercetin-3-O-rhamnoside (quercitrin)
(IC50 = 0.67 mM) and kaempferol-3-O-rhamnoside (afzelin) (IC50 = 2.8 mM). The
combination of quercitrin and afzelin caused synergistic inhibitory activity on
angiotensin converting enzyme (Hansen et al. 1996). Hansen et al. (1996) concluded
that this laboratory study tend support the traditional use of E. laurifolium as a
diuretic (and therefore an anti-hypertensive remedy) by the herbalists at Reunion
Island and Mauritius. The presence of phenols, flavonoids, saponins, tannins,
kaempferol, myricetin and quercetin were also reported (Neergheen et al. 2005).
Anti-microbial screening of E. laurifolium methanolic extracts showed inhibi-
tory activity on Salmonella enteritidis, Pseudomonas aeruginosa, Enterobacter
cloacae, Bacillus subtilis, Scleorotinia sclerotium and Candida albicans using the
disk diffusion method (Rangasamy et al. 2007). The minimum inhibitory concentra-
tions of E. laurifolium methanolic extracts on Bacillus subtilis, Candida albicans
and Salmonella enteritidis were 1000, 125 and 500 μg/ml respectively (Rangasamy
et al. 2007). The crude methanolic extract of E. orientale leaves was reported to
possess α-amylase (IC50 value 7472.92 ± 5.99 μg/ml compared to acarbose
1100.06 ± 0.03 μg/ml) and α-glucosidase (1.02 ± 0.02 μg/ml compared to acarbose
5115.73 ± 3.91 μg/ml) inhibitory properties. The extract exhibited an uncompetitive
and mixed type of inhibition on α-glucosidase and α-amylase respectively (Picot
et al. 2014). The capacity of E. laurifolium to inhibit lipid peroxidation was demon-
strated using 2 systems namely, Fe3+/ascorbate system (IC50 0.0435 ± 0.001 mg FW/
ml) and 2, 2′-azobis (2-amidinopropane) dihydrochloride system (IC50
0.05 ± 0.002 mg FW/ml) (Soobrattee et al. 2008).
Faujasiopsis flexuosa (Lam.) C. Jeffrey (Asteraceae)
Faujasiopsis flexuosa is an endemic plant of Mauritius traditionally used for the

treatment and management of dysentery and diabetes. The use of medicinal plants
is well anchored in the Mauritian culture. In the quest for therapeutic agents the
study of local folk plants has gained much momentum. Based on ethnopharmaco-
logical data of the traditional use of F. flexuosa, scientists have attempted to validate
its hypoglycemic and antimicrobial properties, hereby justifying its medicinal vir-
tues. It was suggested that the possible presence of polyines and alkaloids might
justify F. flexuosa antimicrobial and hypoglycemic properties. This monograph
appraises the pharmacological studies undertaken in order to rationalize the use of
F. flexuosa in traditional medicine and provides an account of its botanical descrip-
tion and ethnopharmacological uses.
Faujasiopsis flexuosa originally known as Eupatorium flexuosum Lam. (GBIF
2015) is a tropical plant of the Asteraceae family endemic to Mauritius (Mootoosamy
and Mahomoodally 2014). The genus Faujasiopsis consists of 3 species which
belong to the Mascarene has Archipelago (Gurib-Fakim and Brendler 2004a, b).
The species flexuosa and erecta are commonly found in Mauritius and bourbonensis
originates from Réunion Island (Gurib-Fakim and Brendler 2004a, b). Commonly
known as ‘Bois cassant’ in Mauritius (Mootoosamy and Mahomoodally 2014;
Gurib Fakim and Brendler 2004a, b). F. flexuosa is found in humid forests at medium
altitudes (600–700 m) (Gurib-Fakim and Brendler 2004a, b). Other vernacular
names commonly used in Réunion island include ‘zigzag’ and ‘petit café’ (CIRAD
2008). The plant was named after Faujas de Saint Fond the senior curator of the
Natural History Museum in Paris and the epithet ‘flexuosa’ came from the latin
word ‘flexus’ meaning ‘full of bends’ (Gurib-Fakim and Brendler 2004a).
F. flexuosa is presented as an erected or lianescent shrub of 1–2 m tall (Gurib-
Fakim and Brendler 2004a, b; CIRAD 2008). The slender spreading branches are
easily breakable (Gurib-Fakim and Brendler 2004a, b). The petioled leaves measur-
ing between 6 to 17 cm long and 2 to 5 cm wide are ovale to lanceolate and have
serrated to denticulated margins (Gurib-Fakim and Brendler 2004a, b) which turn
slightly purple when mature (CIRAD 2008). Flower clusters are numerous and
appear at branches ends (Gurib-Fakim and Brendler 2004a, b). Flower heads are of
disc shape and the white corollas measure between 3 and 3.6 mm long.
The leaves and the whole plant of F. flexuosa were used by folk Mauritians for
the treatment of diabetes, asthma and dysentery (Mootoosamy and Mahomoodally
2014; Gurib-Fakim and Brendler 2004a, b). Several preparations have been used for
the treatment of diabetes.
Leaves of F. flexuosa are known to contain alkaloids, phenols, flavonoids, antho-
cyanins, anthraquinones, tannins, sterols, terpenes, triterpenes and traces of sapo-
nins (Gurib-Fakim and Brendler 2004a, b; Mahomoodally et al. 2012a; Picot et al.
2014). It was also reported that polyines present in several species of the Asteraceae
family might be responsible for antibacterial activity against gram positive and
gram negative bacteria and also viruses (Gurib-Fakim and Brendler 2004a, b). The
hypoglycemic and antiasthmatic properties of F. flexuosa might be attributed to the

presence of alkaloids in the plant (Gurib-Fakim and Brendler 2004a, b).
The crude water fraction of stem and leaves of F. flexuosa showed potent inhibi-
tory effects on Gram negative bacteria namely Escherichia coli (minimum inhibi-
tory concentration [MIC] = 4 mg/ml), Pseudomonas aeruginosa (MIC = 8 mg/ml),
Salmonella typhimurium (MIC = 4 mg/ml) and Gram positive bacterium
Staphylococcus aureus (MIC = 4 mg/ml). Stem methanol: chloroform (1:1) frac-
tion, leaf hexane fraction, leaf methanol: chloroform (1:1) fraction and leaf metha-
nol fraction inhibited fungi Candida albicans (MIC = 16 mg/ml) (Narod et al.
2004).
The antioxidant potential of crude extracts and fractions of F.flexuosa leaves was
validated using standard in vitro assays. Crude water/methanolic extracts, dichloro-
methane/ethyl acetate/n-butanol and water fractions of F. flexuosa leaves showed
IC50 values ranging between 3.39 to 4.87 μg/ml, 4.95 to 37.01 μg/ml, 6.06 to
10.30 μg/ml and 3.89 to 6.13 μg/ml on 2,2-diphenyl-1-picrylhydrazyl, superoxide
radical, nitric oxide and lipoxygenase respectively. IC50 values of F. flexuosa for
DPPH, SO and NO were comparable to the control, ascorbic acid. F. flexuosa crude
extracts and fractions showed IC50 values lower than the control quercetin on lipox-
ygenase (Mahomoodally et al. 2012b).
The immunomodulating capabilities of F. flexuosa extracts were assessed by
evaluating the immune response of phagocytes and monocytes at different steps.
The inhibitory activity of F. flexuosa crude methanolic extract on whole blood
phagocytes for reactive oxygen species production was 88.7%, 72.4% and 60.7% at
100, 50 and 25 μg/ml respectively. It was suggested that F.flexuosa inhibited bio-
chemical targets such as NADPH oxidase enzyme or scavenge reactive oxygen spe-
cies rather than affecting a specific transductional pathway (Mahomoodally et al.
2012c).
The inhibitory potential of the extracts F. flexuosa leaves on α-amylase was vali-
dated using in vitro assays. Crude water/methanolic extracts, dichloromethane/
ethylacetate/n-butanol and water fractions gave IC50 values ranging from
27.36 ± 4.17 to 384.72 ± 39.9 μg/ml. Ethyl acetate fraction (IC50 value
27.36 ± 4.17 μg/ml) showed best inhibitory activity on α-amylase with an IC50 value
significantly (P < 0.05) lower than the known antidiabetic drug acarbose (IC50 value
75.86 ± 8.16 μg/ml). Further investigation on the activity of F. flexuosa ethylacetate
fraction on amylase activity in mouse plasma showed that F. flexuosa inhibited amy-
lase activity significantly (P < 0.05) from 7.54 to 87.39% with increasing extract
concentration (100–800 μg/ml) (Mahomoodally et al. 2012a). No significant inhibi-
tion on α-glucosidase was reported (Mahomoodally et al. 2012a; Picot et al. 2014).
The possible glucose diffusion retardation properties of crude methanolic and
aqueous extracts of F. flexuosa leaves were also investigated using a simple in vitro
dialysis model. It was reported that both extracts (concentration of glucose in exter-
nal solution after 4 hr: 0.738 ± 0.020 mM and 0.732 ± 0.009 mM for methanolic and
aqueous extract respectively) retarded glucose diffusion significantly (P < 0.05)
compared to the negative control experiment (concentration of glucose in external
solution after 4 hr: 0.906 ± 0.015 mM) (Picot et al. 2014). These findings tend to
suggest that F.flexuosa exerts hypoglycemic activity by inhibiting α-amylase, a key

carbohydrate hydrolyzing enzyme and impeding glucose diffusion.
Additionally, it was reported that F.flexuosa crude extracts and fractions pos-
sessed antiglycation activities as compared to the negative control experiment and
comparable (P < 0.05) to the known antiglycation drug aminoguanidine. IC50 values
ranged from 25.31 ± 1.98 to 69.54 ± 2.32 μg/ml (Mahomoodally et al. 2012b).
Toddalia asiatica Lam. (Rutaceae)
Toddalia asiatica is indigenous to the Mascarene region and is also found in Malawi
(Morris 1996) and Mozambique. It is fairly common and is found in numerous loca-
tions from sea level to high altitude forests. T asiatica is a scandent shrub or woody
liana, bearing short, retrorse, dark brown prickles, usually pubescent to shortly his-
pid on younger parts, sometimes glabrous. The leaves are mainly consumed as rem-
edies in traditional medicines. Two teaspoons of the root decoction, along with the
leaves and root bark of Rhus vulgaris, the root bark of Symphonia globulifera, are
drunkonce daily against colds (Neuwinger 1996). A bark maceration is used against
blenorrhagia (gonorrhoea). The leaves and twigs are boiled and the vapours inhaled
against bronchial diseases and malaria. The fruit or fruit decoction is used against
cough, colds, and stomach pain. A decoction of the rootsap is taken as an emetic and
against paralysis resulting from snake bites. The root sap is also applied to scarifica-
tion resulting from snake bites. The root decoction is also drunk against cardiac and
back pain, and also when there are digestive troubles resulting from over consump-
tion of meat. The root decoction is also taken in the event of rheumatism and steril-
ity. A poultice made from the root is applied to swollen limbs. The leaves are boiled
with butter and drunk against pneumonia and rheumatism. A glassful of the leaf
decoction is drunk twice daily against rheumatic pains. This decoction is also gar-
gled against toothache. The leaves are also chewed against toothache, and a decoc-
tion of the aerial parts of the plant is drunk against malaria. A poultice made from
the ground leaves is applied on scabies. Boiled in water, the decoction made from
the ground leaves is drunk against snake bite. The plant resin is drunk by pregnant
women as an oxytocicagent (Kokwaro 1976; Neuwinger 1996).
The phytochemicals compounds isolated are; Coumarins (toddaculin, coumur-
rayin, toddanone, 8-(3,3-dimethylallyl)-6,7-dimethoxycoumarin, isopimpinellin,6-
(3-chloro-2-hydroxy-3-methylbutyl)-5,7-dimethoxycoumarin,6-formyllimettin,5,7,8-
trimethoxycoumarin, toddasin,(+)-toddanol, 6-(2-hydroxy- 3-methoxy-3-
methylbutyl)-5,7-dimethoxycoumarin,and toddalolactone); five new coumarins
(toddalenol, toddalosin, 5-methoxysuberenon, toddalenone, and 8-formyllimettin);
alkaloids (benzo-phenanthridine, alkaloids (des-N-methylchelerythrine, oxycheler-
ythrine, arnottianamide, oxyavicine, avicine, chelerythrine and chelerythrine-psi-
cyanide); four known quinoline alkaloids (N-methylflindersine,4-methoxy-1-
methyl-2-quinolone, skimmianine, integriquinolone); and one known triterpenoid
(amyrin). In vitro assay of the root bark of T. asiatica has shown significant activity
against the malarial vector Plasmodium falciparum. Bioguided fractionations of the
bioactive extracts has revealed that the alkaloid nitidine, as well as other coumarins
present in the plant (Gakunju et al. 1995; Oketch-Rabah et al. 2000), is largely
responsible for this activity. Nitidine has previously received considerable attention
as it is a well-known cytotoxic agent, particularly against leukaemia (Gakunju et al.
1995). Dihydronitidine, on the other hand, has also been reported to exhibit tumour-
specific cytotoxicity in vitro (Iwasaki et al. 2006).
The aerial parts of T. asiatica var. floribunda have been tested for spasmolytic
activity in vitro on guinea-pig ileum. Although the activity was significant, it was
not attributed to the presence of the coumarins toddalolactone and toddanone, as
previously reported. Attempts have been made to assign this activity to the presence
of chlorocoumarin in vitro (Rastogi and Mehrotra 1998). However, these observa-
tions would justify the use of this plant against colics (Lakshmi et al. 2002).
Chinese scientists have tested the plant extracts on the cardiovascular system and
haveisolated an active constituent, which has been identified to be isopimpinellin
(Guo et al. 1998). The wood extract yielded seven compounds having strong anti-
platelet aggregation activity in vitro (Tsai et al. 1998). The aqueous leaf extracts
have also been tested for their antifeedant activity, and a 1% concentration showed
86.1% mortality (Sundararajan and Kumuthakalavalli 2001).
The essential oil extracted from the leaves (Veeramuthu et al. 2006) and the leaf
extracts (Duraipandiyan et al. 2006) inhibited the growth of several bacteria in vitro,
namely Bacillus subtilis, Staphylococcus aureus and S. epidermidis, among others
T. asiatica has also been shown to have anti-inflammatory and analgesic effects in
rats, and it appears that long-term administration does not cause injury to the liver
(Hao et al. 2004).
Recent in vivo tests on mice using extracts of the root bark of T. asiatica has
shown that the latter shows remarkable suppression of parasitaemia (38–66%)
either alone or in combination with chloroquine. The survival rate of the mice was
found to be longer than that of controls (Muregi et al. 2007a, b).
3 Conclusion
The tropical island of Mauritius is considered as a biodiversity hotspot in the Indian

Ocean. It is currently the habitat of around 700 species of indigenous and endemic
plants. Interestingly, the local inhabitants of Mauritius hold an inherent use of
medicinal plants emanating from their ancestors for decades. Nonetheless, there is
still a dearth of scientific validation and compilation of some indigenous plants in
common use. Hence, such practice need proper documentation, conservation and
scientific validation.
References
Adjanohoun E, Ahyi MRA, Eymê J, Gassita JN, Goudotê E, Guêho J, Ip FSL, Jackaria D,
Kalachand SKK, Keita A, Koudogbo B, Landreau B, Owadally AW, Soopramanien A (1983)
Contributions aux etudes ethnobotaniques et floristiques à Maurice (Ile Maurice et Rodrigues).
A.C.C.T, Paris
Boiteau-Allorge L (1999) CD-ROM-Plantes Medicinales de Madagascar. Editions Lune Rouge,
Paris
Centre de cooperation international en recherché agronomique pourle développement (CIRAD)
(2008) Faujasiopsis flexuosa (Lam.) C. Jeffrey. http://arbres-reunion.cirad.fr/especes/astera-
ceae/faujasiopsis_flexuosa_lam_c_jeffrey. Accessed 7 June 2016
Centre de cooperation international en recherché agronomique pour le développement. Antidesma
madagascariense Lam (2006) http://arbres-reunion.cirad.fr/especes/euphorbiaceae/antidesma_
madagascariense_lam. Accessed 7 Jun 2016
CSO (2014) Central Statistical Office, Mauritius
Danthu P, Lubrano C, Flavet L (2010) Biological factors influencing production of Xanthones in
Aphloia theiformis. Chem Biodivers 7:140–150
Daruty DC (1886) Plantes Médicinales de Maurice. Port Louis, Maurice
Duraipandiyan V, Ayyanar M, Ignacimuthu S (2006) Antimicrobial activity of some ethnomedici-
nal plants used by Paliyar tribe from Tamil Nadu, India. BMC Complement Altern Med 6:35
Gakunju DMN, Mberu EK, Dossaji SF, Gray AI, Waigh RD, Waterman PG, Watkins WN (1995)
Potent antimalarial activity of the alkaloid nitidine isolated from a Kenyan herbal remedy.
Antimicrob Agents Chemother 39(12):2606–2609
Global Biodiversity Information Facility (GBIF) (2015) Faujasiopsis flexuosa (Lam.) C. Jeffrey.
http://www.gbif.org/species/107958745. Accessed 7 Jun 2016
Gopalsamy N, Vargas D, Guého J, Ricaud C, Hostettmann K (1988) Saponins from the leaves of
A. theiformis. Phytochemistry 27:593–3595
Guo S, Li S, Peng Z, Ren X (1998) Isolation and identification of active constituent of Toddalia
asiatica in cardiovascular system. Zhong Yao Cai 21:515–516
Gurib-Fakim A (2011) Medicinal plants of Mauritius and of the world. Graphic Press Ltd, Baie
du Tombeau
Gurib-Fakim A, Brendler T (2004a) Medicinal and aromatic plants of Indian Ocean Islands:
Madagascar, Comoros, Seychelles and Mascarenes. MedpharmSci Publication, Stuttgart
Gurib-Fakim A, Brendler T (2004b) Medicinal plants of Indian Ocean Islands. MedPharm
Scientific Publishers, Stuttgart
Gurib-Fakim A, Gueho J (1996) Plantes Médicinal de Maurice, TOME2. Edition de L’Ocean
Indien, Rose-Hill, pp 71–77
Gurib-Fakim A, Guého J (2000) Inventaire et Etudes des Plantes Aromatiques et Médicinales des
Etats du Sud-Ouest de l’Océan Indien. CD-ROM, COI/UE, Nouveaux Territoires, St. Denis,
La Réunion (France)
Gurib-Fakim A, Subratty H, Narod F, Govinden-Soulange J, Mahomoodally F (2005) Biological
activity from indigenous medicinal plants of Mauritius. Pure Appl Chem 77(1):41–51
Hansen K, Adsersen A, Smitt UW, Nyman U, Christensen SB, Schwartner C, Wagner H (1996)
Angiotensin converting enzyme (ACE) inhibitory flavonoids from Erythroxylum laurifolium.
Phytomedicine 2(4):313–317
Hao XY, Peng L, Ye L, Huang NH, Shen YM (2004) A study on anti-inflammatory and analgesic
effects of alkaloids of Toddalia asiatica. Zhong Xi Yi Jie He Xue Bao 6:450–452
Hoffman P (1999) Kew bulletin. Royal Botanic Gardens, Kew
Hollup O (1994) The disintegration of caste and changing concepts of Indian ethnic identity in
Mauritius. Ethnology 33:297–316
Hollup O (2012) Nurses in Mauritius motivated by extrinsic rewards: a qualitative study of factors
determining recruitment and career choices. Int J Nurs Stud 49:1291–1298
Hsoidrou S, Bousta D, Lachkar M, Said Hassane SO, El Youbi-Hamsas A, Mansouri LEL, Benjilali
J, El-Hajaji H, Farah A (2014) Phytochemical screening, immunomodulatory and anti-
inflammatory activities of the phenolic fraction of Aphloia theiformis (Vahl) Benn. Plant used
in comorian traditional medicine. Phytothérapie 21:354–359. doi:10.1007/s10298-014-0875-1
Iwasaki H, Oku H, Takara R, Miyahira H, Hanashiro K, Yoshida Y, Kamada Y, Toyokawa T, Takara
K, Inafuku M (2006) The tumor specific cytotoxicity of dihydronitidine from Toddalia asiatica
Lam. Cancer Chemother Pharmacol 58:451–459
Jolly A, Sussman RW, Koyama N, Rasamimanana H (2006) Ring tailed lemur biology: Lemur
catta in Madagascar. Springer, Spring Street, New York. http://books.google.mu/books?id=
WQ5yWlEJVVYC&pg=PA146&dq=antidesma+madagascariensis&hl=en&sa=X&ei=HSni
UuGnKu6e7AaTkYHwBg&ved=0CCsQ6AEwAA#v=onepage&q=antidesma%20madagas-
cariensis&f=false. Accessed 7 Jun 2016
Joseph A, Troester B (2012) Can the Mauritian miracle continue? The role of financial and ICT
services as prospective growth drivers. Working Paper No. 01/2013, Berlin
Kokwaro JO (1976) Medicinal plants of East Africa. University of Nairobi Press, Nairobi
Kuete V (2013) Medicinal plant research in Africa. Elsevier, London
Lakshmi V, Kapoor S, Pandey K, Patnaik GK (2002) Spasmolytic activity of Toddalia asiatica var.
floribunda. Phytother Res 16:281–282
Lavergne R (1990) Tisaneurs et Plantes Médicinales Indigènes de l’Ile de La Réunion. Editions
Orphie, Chevagny sur Guye
Mahomoodally F, Gurib-Fakim A, Subratty AH (2006) Stimulatory effects of Antidesma madagas-
cariense on D-glucose, L-tyrosine, fluid and electrolyte transport across rat everted intestine,
comparable to insulin action in vitro. Br J Biomed Sci 63(1):12–17
Mahomoodally F, Mesaik A, Choudhary MI, Subratty AH, Gurib-Fakim A (2012a) In vitro modu-
lation of oxidative burst via release of reactive oxygen species from immune cells by extracts of
selected tropical medicinal herbs and food plants. Asian Pac J Trop Med 5:440–447
Mahomoodally FM, Subratty AH, Gurib-Fakim A, Choudhary MI (2012b) Antioxidant, antiglyca-
tion and cytotoxicity evaluation of selected medicinal plants of the Mascarene Islands. BMC
Complement Altern Med 165:1–12
Mahomoodally MF, Subratty AH, Gurib-Fakim A, Choudhary MI, Khan SN (2012c) Traditional
medicinal herbs and food plants have the potential to inhibit key carbohydrate hydrolyzing
enzymes in vitro and reduce postprandial blood glucose peaks in vivo. Sci World J 12:1–9
Mahomoodally MF, Korumtollee HN, Chady ZZBK (2015) Ethnopharmacological uses of
Antidesma madagascariense Lam. (Euphorbiaceae). J Intercult Ethnopharmacol 4(1):86–89
Ministry of Agriculture and Natural resources (1996) Mauritius: Country report to the FAO inter-
national technical conference on plant genetic resources. http://www.fao.org/fileadmin/tem-
plates/agphome/documents/PGR/SoW1/asia/MAURITIU.PDF. Accessed 7 Jun 2016
Ministry of Agro Industry and Food Security (MOAIFS) (2012) Forestry-indigenous and endemic
plants. Republic of Mauritius. http://agriculture.gov.mu/English/Pages/Forestry/Indigenous-
and-Endemic-Plants.aspx. Accessed 7 Jun 2016
Mootoosamy A, Mahomoodally MF (2014) Ethnomedicinal application of native remedies used
against diabetes and related complications in Mauritius. J Ethnopharmacol 10:413–444
Morris B (1996) Chewa medical botany: a study of herbalism in Malawi, Monographs from the
International African Institute. LIT Verlag, Hamburg, pp 227–228
Muregi FW, Ishih A, Suzuki T, Kino H, Amano T, Mkoji GM, Miyase T, Terada M (2007a) In
vivo antimalarial activity of aqueous extracts from Kenyan medicinal plants and their chloro-
quine (CQ) potentiation effects against a blood-induced CQ resistant rodent parasite in mice.
Muregi FW, Ishih A, Miyase T, Suzuki T, Kino H, Amano T, Mkoji GM, Terada M (2007b)
Antimalarial activity of methanolic extracts from plants used in Kenyan ethnomedicine
and their interactions with chloroquine (CQ) against a CQ-tolerant rodent parasite in mice.
Narod FB, Gurib-Fakim A, Subratty AH (2004) Biological investigations into Antidesma mada-
gascariense Lam. (Euphorbiaceae), Faujasiopsis flexuosa (Lam.) C. Jeffrey (Asteraceae),
Toddalia asiatica (L.) Lam. and Vepris lanceolata (Lam.) G. Don (Rutaceae). J Cell Mol Bio
3:15–21
Neergheen VS, Bahorun T, Soobrattee MA, Aruoma OI (2005) A survey of the Mauritian endemic
flora for potential prophylaxis. Food and Agricultural Research Council, Réduit, Mauritius.
http://farc.govmu.org/English/Documents/Policy%20and%20promotion/information%20out-
reach/MAS20051stDraft.pdf. Accessed 7 Jun 2016
Neuwinger HD (1996) African Ethnobotany: poisons and drugs. CRC Press, Boca Raton
Oketch-Rabah HA, Mwangi JW, Lisgarten J, Mberu EK (2000) A new antiplasmodial coumarin
from Toddalia asiatica roots. Fitoterapia 71:636–640
Osorio AA, Lope MR, Jimenez IA, Moujir LM, Rodriguez M, Bazzocchi IL (2014) Elaeodendron
orientale as a source of cytotoxic cardenolides. Phytochemistry 105:60–67
Paris RR, Etchepare S (1964) A propos de la structure de l’aphloiol, parenté de cettexanthone avec
la Mangiférine. Comptes Rendus de Académie des Sciences 258(21):5277–5279
Patrimoine Réunion (2015) La flore de La Réunion, Elaeodendron orientale Jacq. http://
patrimoine-reunion.org/patrimoine-mondial/notre-flore/details/1/37. Accessed 7 Jun 2016
Pernet R (1957) Les Plantes Médicinales Malgaches. Catalogues de nos connaissances chimiques
et pharmacologiques. Mémoires de l’Institut Scientifiques de Madagascar, Série B-Tome VIII
Picot CMN, Subratty AH, Mahomoodally FM (2013) Phytochemical profile and antioxidant prop-
erties of six medicinal plants traditionally used in the management of diabetes in Mauritius.
Pharmacologia 5:42–49
Picot CMN, Subratty AH, Mahomoodally MF (2014) Inhibitory potential of five traditionally
used native antidiabetic medicinal plants on α-amylase, α-glucosidase, glucose entrapment and
amylolysis kinetics in vitro. Adv Pharmacol Sci 2014:1–7
Rangasamy O, Raoelison G, Rakotoniriana FE, Cheuk K, Urverg-Ratsimamanga S, Quetin-
Leclercq J, Gurib-Fakim A, Subratty AH (2007) Screening for anti-infective properties of sev-
eral medicinal plants of the Mauritians flora. J Ethnopharmacol 109:331–337
Rastogi RP, Mehrotra BN (1998) Compendium of Indian medicinal plants. Central Drug Research
Institute, Lucknow
Research and Information System for Developing Countries (2011) Asian biotechnology and
development review http://www.ris.org.in/images/RIS_images/pdf/abdr-nov-mail.pdf.
Accessed 7 Jun 2016
Richards A (2012) Mauritius- Réunion-Rodrigues. Globe Pequot Press Inc, USA. https://books.
google.mu/books?id=JnIb2OCzDvEC&pg=PA8&lpg=PA8&dq=erythroxylum+laurifolium
+%2B+medicinal+properies&source=bl&ots=QbR4NpESR4&sig=nUJevEf7hos5CafiBox
hG2sPi8M&hl=en&sa=X&ei=u8f2VOP3BsP1Uu2CguAC&redir_esc=y#v=onepage&q=
erythroxylum%20laurifolium%20%2B%20medicinal%20properties&f=false. Accessed 7 Jun
2016
Seebaluck R, Gurib-Fakim A, Mahomoodally F (2015) Medicinal plants from the genus Acalypha
(Euphorbiaceae)–a review of their ethnopharmacology and phytochemistry. J Ethnopharmacol
159:137–157
Smadja J, Vera R (1991) Essais préliminaires sur quelques plantes de la Reunion. Plantes
Médicinales et Phytothérapie 25(4):212–241
Soobrattee MA, Bahorun T, Neergheen VS, Googoolye K, Aruoma OI (2008) Assessment of
the content of phenolics and antioxidant actions of the Rubiaceae, Ebenaceae, Celastraceae,
Erythroxylaceae and Sterculaceae families of Mauritian endemic plants. Toxicol In Vitro
22:45–56
Sreekeeson DP, Mahomoodally MF (2014) Ethnopharmacological analysis of medicinal plants
and animals used in the treatment and management of pain in Mauritius. J Ethnopharmacol
157:181–200
Sundararajan G, Kumuthakalavalli R (2001) Antifeedant activity of aqueous extract of Gnidia

glauca Gilg. and Toddalia asiatica Lam. on the gram pod borer, Helicoverpa armigera (Hbn).
J Environ Biol 22:11–14
Sussman LK (1980) Herbal medicine in Mauritius. J Ethnopharmacol 2:259–269
Tchinda AT, Teshome A, Dagne E, Arnold N, Wessjohann LA (2006) Squalene and amentoflavone
from Antidesma laciniatum. Chem Soc Ethiop 20(2):325–328
Tisanes Indigènes (2014) Erythroxylum laurifolium Lam., Erythroxylaceae, Endémique. http://
www.tisanes-indigenes.re/wp-content/uploads/2014/08/Erythroxylum-laurifolium.pdf.
Accessed 7 Jun 2016
Tsai IL, Wun MF, Teng CM, Ishikawa T, Chen IS (1998) Anti-platelet aggregation constituents
from Formosan Toddalia asiatica. Phytochemistry 48:1377–1382
Veeramuthu D, Muniappan A, Savarimuthu I (2006) Antimicrobial activity of ethnomedicinal
plants used by Paliya tribe from Tamil Nadu, India. BMC Complement Altern Med 6:35
Wong Ting Fook WTH (1980) The medicinal plants of Mauritius. ENDA, Dakar
World Health Rankings (2014) http://www.worldlifeexpectancy.com/mauritius-life-expectancy.
Accessed 8 June 2016
Some Wild Herbaceous Plants of Nigeria:
A Biological, Pharmacognostic
and Phytochemical Review
Sunday E. Atawodi, Mubarak L. Liman, Olufunsho D. Olowoniyi,

and Godwin O. Adejo
Abstract In the twentieth century, science makes substantial breakthrough in man-

agement and cure of diseases by chemotherapy due to discovery of antibiotics and
other chemotherapeutic agents. In the twenty first century, man and other animals
are still, however being challenged by emergence of new infectious and non-
infectious diseases that have proven to be resistant to the available novel therapeutic
drugs. The available orthodox medications have either been ineffective against
some causative agents of these diseases; too costly or basically unavailable to the
average citizen of developing countries like Nigeria. The use of herbal remedies for
prevention, management and cure of diseases is as old as antiquity, yet still common
practice among the African population. A number of plants used in traditional herbal
medicine have been evaluated by different researchers. This book chapter provides
a concise review of traditional use, phytochemical contents, pharmacognostic and
biological activities of some wild herbaceous plants that of medicinal importance in
Nigeria. Plants including; Cuminium cyminum Linn, Rauwolfia vomitoria Afzel,
Cassia sieberiana D.C, Piliostigma thonningii (Schumach.) Milne-Redh, Guiera
senegalensis J.F.Gmel, Acalypha indica Linn, Euphorbia hirta Linn, Euphobia uni-
spina N.E.Br, Phyllantus muellerianus (Kuntze) Exell, Senna occidenatlis Linn and
Grewia mollis Juss are comprehensively reviewed. Phytochemicals like glycosides,
alkaloids, polyphenols, flavonoids, phytosterols, terpenoids, saponins etc are widely
reported to be present in various parts of these plants. The traditional herbal use of
the plants in management and cure of ailments, as well as reported biological and
pharmacological activities were linked to the presence of these phytochemicals.
Based on the information reviewed, it is concluded that these wild herbaceous plants
are potential sources of new natural bioactive substances that could be explored and
exploited for their therapeutic and industrial applications.
Dedicated to Our Teachers and Mentors for Enduring Knowledge Impacted

S.E. Atawodi (*) • M.L. Liman • O.D. Olowoniyi • G.O. Adejo
Biochemistry Department, Ahmadu Bello University, Zaria, Nigeria

DOI 10.1007/978-94-024-1120-1_10
250 S.E. Atawodi et al.
Keywords Herbaceous plants • Nigeria • Wild plants • Pharmacognostic effects •

Phytochemicals • Traditional medicine • Herbal medicine
1 Introduction
For time in-memoriem plants have been a source of food and therapeutic remedy for
humans and his animals. Traditional societies in Africa and elsewhere have always
used herbs to promote healing (Atawodi et al. 2009, 2011; Ene et al. 2009; Focho
et al. 2009). Even presently, traditional medicine is still the predominant means of
health care delivery in many developing countries where larger percentage of their
total population depends on it for their well being (Atawodi et al. 2014a, b, c).
In addition, plants are important source of food as well as industrial raw materials
for various products, and are also the basis for the development of many orthodox
drugs. The medicinal values of plants lie in their component phytochemicals such as
different classes of alkaloids, tannins, flavonoids and other phenolic compounds,
which produce definite physiological actions on animal and human body (Ullah and
Khan 2008; Atawodi and Ogunbusola 2009; Ogbe et al. 2009; Asmah et al. 2006).
Nigeria is situated in the Western part of Africa, with coastal boundary delimited
by the Gulf of Guinea in the south, and shared land boundary with Cameroon and
Chad in the east, Niger Republic in the north and Benin in the west. Nigeria covers
a total area of 923.758 km2 with a coastline of 853 km and land boundry of 4047 km.
The latitudinal and longitudinal extent of the country is 4° to 14° N and 2° to 15° E
making it one of the few countries in the world to have both tropical and sub-Saharan
vegetation. As a tropical country, Nigeria is blessed with an enormous biodirversity
of natural resources but unfortunately receiving an unfair share of the sun’s ultravio-
let radiation in addition to the tropical ecosystem’s myriad of pathogenic microbes,
as well as man-made disease causing agents like tobacco (Atawodi et al. 1995).
African plants which were able to withstand and survive in these stressful condi-
tions due to their abilities to accumulate biologically active phytochemicals
(Atawodi et al. 2014a), should also be able to produce secondary metabolites that
will protect the population.
From the mangrove rain forest of the south to the sunny, seasonal rainy savannah
in the north, Nigeria is blessed with an enormous plant biodiversity. The presence of
these numerous floras with ancient ethnomedicinal uses in herbal medicine inspired
several studies by various researchers on the biological activities and chemical con-
stituents inherent in these plants (Ashaf and Olunu 2011; Adejo et al. 2015).
However, many of such plants which include large numbers of wild plants whose
phytochemical constituents have been identified and potential biological activities
confirmed are yet to be exploited pharmaceutically or industrially. This chapter
attempts to concisely provide an update on the traditional use, phytochemical con-
tents, pharmacognostic and biological activities of some Nigerian plants with par-
ticular reference to wild herbaceous plants.
Some Wild Herbaceous Plants of Nigeria: A Biological, Pharmacognostic… 251
Pictures 1 and 2 Cuminia cyminum J.F. Gmel (Source: http://magicgardenseeds.com)
Apiaceae
Cuminium cyminum Linn. Synonyms: Cuminia cyminum J.F. Gmel., Cuminum

aegyptiacum Merax ex DC., Cuminum hispanicum Bunge, Cuminum odorum
Salisb., Cuminum officinale Garsault (Inval.), Cuminum sativum J. Sm., Cyminon
longeinvolucellatum St.-Lag., Linguisticum cuminum (L.) Crantz, Selinum cumi-
num E.H. Crantz.
Common Names
Commonly called Cumin (Johri 2011); Cummin, Roman Caraway, Jeera (India)
(Parashar et al. 2014) (Pictures 1 and 2).
Cuminum cyminum is an annual herbaceous plant with a slender branched stem. It
grows up to 30–60 cm tall. The leaf is alternate, simple or compound and have a
sheathing base below. The flowers are small, pink and characteristically borne in
umbrella shaped clusters. The flowers have both male and female structures together
and an inferior ovary that develops into a very characteristic fruit called a cremo-
carp, which is a capsule that upon maturity breaks into two one-seeded bits (Parashar
et al. 2014).
Cuminium cyminum grows in many countries of Africa, as well as Asia and Europe
(Johri 2011)
Cumin needs fairly cool, less humid climate and temperatures of between 25 and
30 °C to grow. It is very sensitive to rainfall, such that rainfalls at times of harvest
can drastically lower yield and crop quality. The plant is also susceptible to frost
damage during flowering and initial stages of seed formation. It grows best on well
drained sandy-loam soil and optimally alkaline pH range of 6.8–8.3 (Anandaraj and
Sastry 2015). Cuminium cyminum has however, been experimented to thrive well in
relatively low temperature zones as higher temperatures lower yields and affects
both seedling weight and height. A temperature range of 15–25 °C has been found
to be the optimum temperature for cumin seed germination rate, optimum seedling
growth, seedling weight and height. With respect to timing, the last week of March –
first week of April was established to be the best planting time, as this period offers
significantly shorter emergence time, highest plant height and yield (Khosh-Khui
and Bonyanpour 2006).
Major Chemical Constituents and Bioactive Compounds
The major phytochemicals occurring in cumin are cuminaldelyde, limonene, α- and
β- pinene, 1,8–cineole, o- and p-cymene, α- and y-terpinene, sufranal and linalool
(Johri 2011). Cumin seeds contain up to 5% of a volatile oil composed primarily of
aldehydes. In addition, the seeds yield 22% fats, numerous free amino acids and a
variety of flavonoid glycosides, including the derivatives of apigenin and luteolin.
The cuminaldelyde content varies considerably, depending on the source of the oil
(Parashar et al. 2014). Ahmad and Saeidnia (2011) also reported monoterpene
hydrocarbons as major components of the plant, while sesquiterpenes are minor
components. The analysis of the volatile oil from the fruit of cumin showed the
presence of trans-dihydrocarvone, y-terpinene, p-cymene, α-phellandrene and
p-menth-2-en-7-ol (Chaudhary et al. 2014).
Traditional Uses, Part(s) Used and Common Knowledge/Uses in Traditional
Medicine
In Tunisian traditional medicine, cumin is considered abortive, galactagogue,
antiseptic and antihypertensive (Leporatti and Ghedira 2009). In Indian traditional
medical practices, cumin seeds are considered carminative, eupeptic, antispasmodic,
and astringent. It is used in Nigeria for the treatment of mild digestive disorders,
diarrhea, dyspepsia, flatulence, morning sickness, colic, dyspeptic headache and
bloating. It promotes the assimilation of other herbs and improves liver function
(Johri 2011). Cumin is also used in bronchio-pulmonary disorders and as a cough
remedy.
Biological Activities
Several studies have verified the antibacterial actions of cumin against a range of
useful and pathogenic gram-positive and gram-negative bacterial strains (DeMartino
et al. 2009). Specifically, Chaudhary et al. (2014) reported the potency of the essen-
tial oil of cumin against Staphylococcus epidermidis, Staphylococcus aureous,
Staphylococcus haemolyticus, Propionibacterium acnes, Corynebacterium
diphtheriae, Erysipelothrix rhusiopathiae, Bacillus cereus, Clostridium tetani,
Clostridium difficile, Escherichia coli, Salmonella typhi, Klebsiella pneumoniae,

Vibrio cholera, Aeromonas hydrophila, Mycobacterium tuberculosis and Neisseria
gonorrhoeae. Bokaeian et al. (2014) have also demonstrated that essential oil
from cumin possess potent antimicrobial activity against multi-drug resistant
Escherichia coli
Antifungal activity of cumin has also been reported against soil, food, animal
and human pathogens including, dermatophytes, Vibrio sp., yeast, aflatoxins and
other mycotoxin-producing fungi (DeMartino et al. 2009; Boyraz and Ozcan 2005).
Using agar-well diffusion method El-Said and Goder (2014) reported that essential
oils of Cuminum cyminum inhibited the growth of twenty six fungal isolates, while
Chaudhary et al. (2014) confirmed the antifungal activity of cumin oil against
Aspergillus niger, Saccharomyces cererisae and Colletrotrichum gloeosporiodes.
Other biological activities reported for cumin include antioxidant, anticarcinogenic,
antimutagenic, antidiabetic, diuretic, immunomodulatory, and estrogenic (Johri
2011).
Apocynacea
Rauwolfia vomitoria Afzel (Synonyms: Rauvolfia stuhlmannii K.Schum)

(Pictures 3 and 4)
Botanical Name and Family
Rauwolfia vomitoria belongs to the family of Apocynaceae, Its common names are
swizzle stick, devil’s pepper (English) and Asofeyeje (Fapojuwomi and Asinwa
2012).
Rauwolfia vomitoria is a shrub or small tree that grows up to 8m, with parts, except
old ones containing latex. The branches are usually whorled, while the nodes are
enlarged and leaves, lumpy in threes, elliptic-acuminated to broadly lanceolate.
Usually, flowers are minute, sweet-scented with branches of inflorescences that are
distinctly superfluous with hardly ant-free corolla lubes, while the fruits are fleshly
and red in colour (Fapojuwomi and Asinwa 2012).
Rauwolfia vomitoria is native to tropical Africa from Senegal east to Sudan and
Tanzania, south to Angola; and naturalized in China, Bangladesh, and Puerto Rico.
Rauvolfia vomitoria occurs naturally in forest, but is mostly found in forest regrowth
where fallow periods are prolonged.
Rauwolfia vomitoria occurs in bush vegetation, gallery forest, secondary vegetation
where fallow periods are long, and along roadsides, from sea-level up to 1600 m
altitude. Rauwolfia vomitoria can be found flowering and fruiting almost through-
out the year, but sometimes not and usually less abundantly during the rainy season.
Pictures 3 and 4 Rauwolfia stuhlmannii (Sources: Authors and prota4u.org)
The flowers are pollinated by insects such as small bees and flies, and the fruits are
dispersed by birds.
Rauwolfia vomitoria contains a large number of indole alkaloids, between 40 and
80. Most occur in very small amounts and several are disputed. Most alkaloids
occur in an unstable complex, and seasonal variation is present as well. Leaves con-
tain 0.03–0.8% total alkaloids, stem bark about 0.6%, roots 0.15–0.2% and root
bark 1.5–2.0%.The alkaloids of Rauwolfia vomitoria can be grouped into 5 main
types: (1) yohimbine and derivatives, including reserpine and deserpidine
(11-demethoxyreserpine); (2) the heteroyohimbine type, including ajmalicine
(raubasine), reserpinine (rescinnamine) and reserpiline; (3) sarpagane derivatives,
including sarpagine (raupine); (4) the dihydro-indole type, including ajmaline;
(5) the anhydronium bases, including alstonine, serpentine and serpenticine. Other
groups include the oxindoles and pseudoindoxyls. Serpentinine is the only dimeric
yohimbin-related alkaloid isolated so far.
In the root bark reserpiline is the major component, followed by reserpine, reser-
pinine and ajmaline. In the stem bark reserpiline is also the major component, with
small amounts of isoreserpiline and yohimbine. The leaves were found to contain
mainly geissoschizol, but no reserpine, reserpinine or ajmaline. The alkaloids in the
leaves comprised about 41% heteroyohimbines and 52% oxindoles. The unripe fruit
contains several alkaloids, amongst which is 2,6-Dimethoxybenzoquinone, a ben-
zoquinone that is a toxic chemical compound (Van-Dilst and Leeuwenberg 1991).
Medicine
In the entire distribution area of Rauwolfia vomitoria, a root decoction, root macer-
ate or powdered root in water is taken to treat diarrhoea, rheumatism, jaundice,
venereal diseases and snakebites. Root products are also widely taken to treat
hypertension, and as a sedative to calm people with epilepsy, and people who are
psychotic or mentally ill; they are also used to wash children with colic or fever.
Externally, macerated or powdered root or sometimes pulped fruit are applied to a
range of skin problems, such as rash, pimples, chicken pox, wounds, scabies, pso-
riasis, leprosy, hemorrhoids, head lice and parasitic skin diseases. A root decoction
is used as a mouth wash against gingivitis or thrush. The stem bark or leaves are also
used for these purposes, but to a lesser extent. The stem bark, leaf decoction and
latex of young twigs are widely used as purgative or emetic (Burkill 2000).
In Guinea the root maceration is applied to tumors. In Liberia a bark infusion is
taken to cure fever. A leaf infusion is rubbed in against yaws. Dried or fresh pulver-
ized roots in palm wine or oil are taken to treat female sterility. A root decoction is
used in massages and baths to treat rheumatism, tiredness and rachitis. In Togo
pulverized root bark in brandy is taken to treat tuberculosis. In Cameroon a decoc-
tion of powdered roots is taken to treat diabetes and malaria. In the Central African
Republic a root decoction is taken to treat hernia. In Côte d’Ivoire leaf sap is rubbed
between the toes to treat infections caused by humidity. A leaf maceration is used
for bathing children with fever. In Gabon chopped and boiled leaves mixed with fat
are applied to the skin to cure rheumatism and sprains. A mixture of pulverized root
or leaf sap with plant oil or lemon juice is applied to the hair to stop hair loss. In
Nigeria the root and leaves in decoction are taken to treat indigestion, as a tonic, and
as an abortifacient. In Equatorial Guinea the latex is used for cicatrization of wounds
(Terashima and Ichikawa 2003).
In the Central African Republic the roots of Rauwolfia vomitoria, alone or
together with seeds of Strophanthus gratus (Wall & Hook) Franch. are pounded to
a paste, which serves as arrow poison. In DR Congo, the roots are a common addi-
tive to Periploca nigrescens Afzel. hunting poison, while in Equatorial Guinea, the
root scrapings are mixed with cassava meal and used as a rat poison. In Nigeria and
other West African countries, the root is considered aphrodisiac when taken in palm
wine (Latham 2004).
Rauvolfia vomitoria is used to treat leprosy in the Democratic Republic of Congo.
The plant is very important and useful in the treatment of lunatic patients; the root
is added to gin and given to mentally ill persons. It can also be ground into powder
and taken with pap, and can be taken in form of decoction used for rheumatic pains.
An infusion of the root bark is used to treat jaundice and gastro-intestinal distur-
bance (Fapojuwomi and Asinwa 2012).
Of the Rauwolfia alkaloids, five are used in medicine: reserpine, reserpinine, deser-
pidine, ajmalicine and ajmaline. There are several patented methods for the extrac-
tion of the main component reserpine. Furthermore, several simple and accurate
methods have been developed to identify Rauvolfia alkaloids, e.g. reserpine, serpen-
tine and ajmaline.
Reserpine is a well-known antihypertensive, antipsychotic and sedative. It is a
sympatholytic agent acting indirectly on the peripheral and central nerve terminals
(Bedu-Addo 1993). It impairs the storage of biogenic amines resulting in depletion
of norepinephrine, dopamine, and serotonin. Depletion of norepinephrine induces a
lasting drop in blood pressure. Contra-indications for using reserpine are depres-
sion, peptic ulcer, and hypersensitivity to the alkaloid, while the side effects of the
medication include drowsiness, nasal congestion, salivary and gastric hypersecre-
tion, paradoxical anxiety, depression and retention of water and Na+. Overdose may
also cause respiratory depression, slowed heartbeat, hypotension, confusion, trem-
ors, convulsions and gastro-intestinal distress. Reserpine has been shown to enhance
the hypoglycaemic effect of insulin and the hyperglycaemic effect of adrenalin, and
has inhibited the physiological hyperglycaemic response in diabetic patients
(Nwodo et al. 2003). Because of the necessary high doses and the resulting danger-
ous side effects, reserpine lost its importance as a medicine. It is only used in low
doses for mild to moderately severe high blood pressure, often together with ajma-
licine. Reserpinine and deserpidine are reserpine analogues. Both alkaloids have the
same effects as reserpine, and can be used to treat the same conditions, while their
side effects are reported to be less pronounced. Reserpiline is marked sympatholytic
and hypotensive with no noticeable depressant effects on the central nervous system
and no sedative properties, and also lacks most of the side effects of reserpine and
its analogues.
Ajmalicine is an α-adrenergic blocking spasmolytic which, at high doses, moder-
ates the activity of the vasomotor centres, especially in the brain stem causing an
increase of the blood flow to the brain. It is mainly used in products that treat the
psychological and behavioural problems associated with senility, stroke and head
injuries. Ajmaline is an anti-arrhythmic, which substantially decreases the rate of
depolarization of artrial and ventricular cells. Its toxicity has limited its uses and it
is mainly prescribed against rapid irregular cardiac beat. Because of its toxicity, it is
no longer marketed in several countries. Several other Rauwolfia alkaloids have
hypotensive or sedative activities, but most are less effective (Nwodo et al. 2003).
An ethanolic leaf extract of Rauwolfia vomitoria caused a reduction in blood
sugar levels of normal and alloxan-induced diabetic rabbits, comparable to that of
tolbutamide, while decoction of the root has no adverse effect on the oestrous cycle,
fertilization or implantation, and also has no effect on the foetus or hormone-
induced infertility in rats. The root bark extract has been reported to show antibacte-
rial, antiprotozaol activity in vitro against several human pathogens (Fapojuwomi
and Asinwa 2012).
Caesalpinacea
Cassia sieberiana D.C (Synonym: Cassia kotschyana Oliv.) (Picture 5)

Cassia sieberiana DC, (family: Caesalpiniaceae) is distributed widely from
Senegal, through Nigeria, and Gambia eastward to DR Congo and Uganda (Van der
Maesen 2007).
Picture 5 Cassia sieberiana (Source: http://www.zimbabweflora.co.zw)
Common Names
African laburnum, West African laburnum (English), Casse du Senegal, Casse de
sieber, Casser agrappes, Casse-flute (French), Mzangaya, Mzangaye (Swahili) (Van
der Maesen 2007).
Cassia sieberiana is an annual plant that grows in the world tropical zones. It is an
upright growing plant that can reach a height of 4 ft with large green leaves and very
bright yellow flowers. The seed range in colour from greenish brown to dark brown
with smooth surfaces and may have small bright colored bands on the outer surface
(Olapade et al. 2014).
Cassia sieberiana is distributed widely from Senegal, through Nigeria and Gambia
east to DR Congo and Uganda (Van der Maesen 2007).
It occurs in tree or shrub savanna with less than 800 mm annual rainfall. Acid sandy
soil is preferred (Van der Maesen 2007).
Cassia sieberiana leaves contain flavones (quercitrin, isoquercetrin), an anthraqui-
none (rhein) and tannins. Anthraquinones and sterols are also present in the root
(Van der Maesen 2007). Polyhydroxy and phenolic substances were identified in the
root extract (Nartey et al. 2012) while the seeds contain tannins, alkaloids, phenols,
oxalate, cardiac glycosides and flavonoids (Olapade et al. 2014).
Cassia sieberiana is included in Nigerian Herbal Pharmacopoeia (NHP) and
West African Herbal Pharmacopoeia (WAHP) (Ajayi et al. 2014).
Traditional Uses, Part(s) Used and Common Knowledge

The entire plant is purgative and diuretic. In Senegal, an infusion of the entire plant
is given against all children’s diseases. Different parts of the plant are applied to
teeth to cure toothache. Across Africa, it is also used to treat skin diseases, malaria,
stomachache, ulcer, diarrhea, gonorrhea and sleeping sickness. The decoction of the
root is used to treat haemorrhoids, bilharzia, dropsy, bloody dysentery and intestinal
worms. The root decoction is also considered an aphrodisiac (Van der Maesen
2007).
In Senegal, the aqueous root extract of C. Sieberiana was used in traditional
medicine to treat pain and inflammation (Kerharo-Adam 1974). The roots, boiled in
water, are used to treat haemorrhoids, bilharzia, leprosy, dropsy and bloody dysen-
tery. In Côte d’Ivoire the decoction is taken in large doses to treat intestinal worms
including tapeworms, although this is risky. An infusion of the root bark is employed
against venereal diseases, sterility and dysmenorrhoea. After soaking the roots in
water, the liquid is used for a bath against tiredness and for body massage. A decoc-
tion of the roots is considered an aphrodisiac. In Burkina Faso a pinch of powdered
dried decorticated roots taken at the end of each meal is said to prevent malaria.
Crushed roots are rubbed on the temples to treat headache. Debarked roots are
boiled with bark of Terminalia macroptera Guill. & Perr. to combat eczema
(Van der Maesen 2007).
Leaf extracts of Cassia sieberiana exhibit antibacterial action against Staphylococcus
lutea, Mycobacterium phlei, Bacillus subtillis and Proteus sp (Van der Maesen
2007). Several compounds exhibiting a broad spectrum antimicrobial activity have
been isolated from Cassia sieberiana. A crude extract of the leaves showed antiplas-
modial activity against Plasmodium including activity against chloroquine-resistant
strains of Plasmodium falciparum, and flavonoids and isoflavonoids have been iso-
lated as active ingredients. The plant has also been reported to have antioxidant,
gastroprotective, laxative, anti-diarrheal anti-inflammatory and analgesic activities
(Asase et al. 2005; Nartey et al. 2012)
Piliostigma thonningii (Schumach.) Milne-Redh (Synonym: Bauhinia thonningii
Schumach. & Thonn.)
Piliostigma thonningii (Schumach.) Milne-Redh is a leguminous plant belonging to
the Caesalpiniaceae family (Jimoh and Oladiji 2005).
Common Names
Camel’s foot, Monkey bread (English), Kalgo (Hausa), Abefe (Yoruba), Okpoatu
(Ibo) (Jimoh and Oladiji 2005) (Picture 6).
Piliostigma thonningii is a deciduous dioecious tree that grows up to 10–40 m tall,
but the bole can be branchless for 2–3 m. The outer bark is rough, longitudinally
fissured and dark brown to grey or black. The leaves are alternate, conspicuously
Picture 6 Piliostigma thonningii (Source: Authors)
bi-lobed for one eighth to one third of the length. The stipules are 3–6 mm long, the
petiole is 2–7 cm long and the blade grows to between 17 and 21 cm, with base,
usually a strong cordate. The apex of lobes are rounded to acute, leathery, upper
surface glabrous. The lower surface has rusty brown crisped hairs and conspicuous
reticulate venation, palmately veined with 11–15 basal veins. Inflorescence is a
panicle, usually alternately leaf-opposed and axillary along branches, male inflores-
cence very narrowly pyramidal, up to 25 cm × 5.5 cm, female inflorescence up to
7 cm long, few flowered. The many-seeded fruit is an oblong to linear-oblong pod,
12–37 cm × 3–7 cm, woody, brown-pubescent when young but later glabrescent,
persisting on the tree but finally decaying on the ground. The seeds are obovoid to
ellipsoid, 4–9 mm × 2–7 mm × 3–4 mm dark brown to blackish, compressed.
Seedling is with epigeal germination (Lemessa 2010).
Commonly called camel’s foot tree, Piliostigma thonningii is native to tropical
Africa, widespread in the Sudano-Guinean region from Senegal eastward to Eritrea
and occurs southward to Namibia, Botswana, Mozambique and South Africa. It also
occurs in Yemen (Lemessa 2010).
Piliostigma thonningii occurs from sea level up to 2200 m altitude in areas with
annual temperature of 20–2 °C, an average rainfall of 400–1500 mm and a dry sea-
son of 6–11 months. It is sensitive to frost, grows on all soil types but heavy clay
soils or medium loamy soils are preferred. Piliostigma thonningii is common in
secondary and gallery forest, woodland, wooded grassland, bush land and also river
valleys (Lemessa 2010).

The phytochemicals contained in Piliostigma thonningii seed include saponins, fla-
vonoids, phenolics, glycosides, anthraquinones and cardiac glycosides. It is also a
good source of antioxidant micronutrients such as iron, calcium, selenium, zinc and
manganese (Jimoh and Oladiji 2005; Bello et al. 2013) Epicatachin and inositol
have been identified in the fruit while saponins, flavonoids, phenolics, anthraqui-
nones and glycosides are in the seeds. The bark contains tannins whereas the leaf
contains 2-phenoxychromone, 6,8-di-C-methylquercetin-3-methyl ether,
6-C-methylquercetin-3,7-dimethyl ether and 6,8-di-C-methylquercetin-3,7-
dimethyl ether. Quercetin and quercitrin are also present in the leaf (Lemessa 2010).
The bark of Piliostigma thonningii is commonly used in tying hut, fence and bridge
building. Fibre extracted from the bark and root is widely used for making string,
rope, and cloth. The fruit is eaten as a snack or as an emergency food, especially by
children and herdsmen. An infusion of the bark, leaf or pods is used to coagulate
Funtumia latex used in making rubber. The leaf extract is effective in treating
inflammations, bacterial infections, worm infestation and to arrest bleeding (Ozolua
et al. 2009).
The root, bark and leaf extracts possess antiviral activity against Herpes simplex
virus type 1 and 2, HIV and different strains of influenza and syncytial viruses. The
root bark also possesses antitussive, anti-inflammatory and analgesic properties.
The stem bark has antibacterial activity against Bacillus subtilis, Corynebacterium
pyogenes, Escherichia coli, Proteus vulgaris, Shigella dysenteriae and
Staphylococcus aureus. The bark extract also have larvicidal and antihelminthic
properties. Butanol and ethylacetate extracts have antioxidant activity (Lemessa
2010).
Uses in Traditional Medicine
A root preparation is applied on wounds and ulcers as a haemostatic and to promote
healing. It is also used as a diuretic and for the treatment of diarrhea, dysentery,
worms and other intestinal problems. Root preparations are also used as remedy for
cough and snake bite (Jimoh and Oladiji 2005). Preparations of the bark are used to
treat pneumonia, skin infections, pains, rheumatism and inflammation. Preparations
of the leaf are used for respiratory problems, as antiseptic and to promote wound
healing. They are also administered to treat epilepsy and heavy menstrual flow
(Lemessa 2010; Bello et al. 2013). The leaf extract is effective in treating inflamma-
tions, bacterial infections, worm infestation and to arrest bleeding (Ozolua et al.
2009).
Picture 7 Guiera senegalensis (Source: Authors)
Combretaceae
Guiera senegalensis J.F Gmel. (Picture 7)

Guiera senegalensis (Family: Combretaceae) is one of the most important West
African medicinal plants often used to treat a variety of microbial infections and the
most frequently used part is the leaf (Silva et al. 2008).
Common Names
Guier du Senegal (French). Sabara (Hausa), Shafi pitu (Marghi), Tadar (Tangale),
Kashishi (Kanuri)
Guiera senegalensis is a shrub that can grow to a height of 3–5 m according to habi-
tat. Its stem presents numerous knots that send out branches. The ash-grey stem and
branches have fibrous and pubescent bark and bear opposing short petiolated oval
leaves, sometimes mucronated, sometimes even cordate, about 2 to 4 cm long by 1
to 2 cm wide. The grey-green leaves, darker on their upper surface display black
spots on their lower surface and are slightly downy on both sides (Somboro et al.
2011).
Guiera senegalensis is a shrub found abundantly in the savannah region of West
Africa (Oshobu and Geidam 2014). It is widely distributed in the savannah region
of West and Central Africa – Nigeria, Senegal, Gambia, Mali, Niger, Burkina Faso
and Ghana (Salihu and Usman 2015).
Guiera senegalensis is is found in shrub savanna, tree savanna and fallow land from
sea level up to 1000 m altitude. It grows in areas with 200–800 mm annual rainfall
and can thrive on all types of soil, but mainly on dry sandy or degraded soils, some-
times in areas which are temporarily flooded. It does not tolerate heavy shading.
Guiera senegalensis is considered an indicator of overgrazing and is very drought
resistant (Sanogo 2012).
Among the phytochemicals identified in different parts of Guiera senegalensis such
as leaves, fruits, root and stem bark are resins, alkaloids, tannins, saponins, glyco-
sides and terpenes (Onwuliri et al. 2009), terpenoids, anthraquinones, coumarins,
flavonoids, cardiotonic and cyanogenic heterosides (Somboro et al. 2011). The gall
extract is particularly rich in flavonoids and polyphenols (Sombie et al. 2011).
Guiera senegalensis is active against cough, respiratory congestion and fever. It is
prescribed as an antitussive, to ease breathing and to treat lung and bronchial disor-
ders and malaria. Various parts are prescribed for stomach pains, dysenteric diar-
rhea, syphilis, beriberi, leprosy and impotence. A decoction of the leaf is applied
against eczema, cold and chest conditions. Veterinary uses of the plant are in diets
designed to increase body weight, reproductive capacity and milk secretion in ani-
mals (Somboro et al. 2011). It is one of the most important West African medicinal
plants often used to treat a variety of microbial infections, including trypanosomia-
sis (Atawodi et al. 1995), and the most frequently used part is the leaf (Silva et al.
2008).
Aqueous (cold and hot) and methanolic extracts of Guiera senegalensis were tested
for antimicrobial efficacy against Staphylococcus aereus, Salmonella typhi,
Escherichia coli, and Streptococcus pyrogenes by determining the minimum inhibi-
tory concentration (MIC) and minimum bactericidal concentration (MBC) of the
extracts using standard methods. Results showed that all the extracts inhibited the
growth of S. aereus, S. typhi and E. coli at all concentrations while S. pyrogenes was
only inhibited at 250 and 200 mg/ml on some of the extracts (Onwuliri et al. 2009).
Other biological activities identified in Guiera senegalensis include antioxidant
(Atawodi and Onaolapo 2010), trypanocidal (Atawodi 2005), effect on central ner-
vous system, on cancer cells and as a snake venom detoxicant (Somboro et al.
2011).
In certain climes, people mix galls from G. senegalensis (gall nuts are frequently
formed on the above-ground parts of the plant) with charcoal to make a highly
diuretic powder prescribed in serious cases of oligouria and even anuria, and in
particular for cerebral malaria. Other people reduce the galls to powder with the pith
from Combretum aculeatum stems and salt. This powder is diluted in water imme-
diately before use and is prescribed for painful stomach cramps with mucous stools
Picture 8 Acalypha
indica (Source: http://
pixgood.com)
and vomiting. A tea made from leaves is prescribed by the oral route (one litre daily)
to treat eczema and is also used against attacks of fever, and to cure chest conditions
and colds. Fresh mashed, chewed or cut leaves when placed on a wound staunch
bleeding. Powdered dried leaves associated with Melanther ascandens are adminis-
tered by the nasal route to treat headaches and sinusitis. The leaves are also used as
a poultice on tumors and against Guinea worm (Somboro et al. 2011).
Euphorbiaceae
Acalypha indica Linn (Synonyms: Acalypha ciliata Wall., Acalypha canescens

Wall., Acalypha spicata Forsk.)
Common Names Three-seeded mercury, Acalypha (Indian), Ricinelle des Indes,
orielle de chatte, herbe chatte (French) (Picture 8)
Acalypha indica belongs to the family Euphorbiaceae and is commonly called
‘Indian acalypha’, or Indian copperleaf (Jagatheeswari et al. 2013).
Acalypha indica is a monecious, annual to sometimes short-lived perennial herb up
to 1.5 to 2.5 m tall. Stems are sparingly to densely hairy and leaves are simple and
arranged spirally.Stipules are linear, about 2mm long and the petiole up to 12 cm
long. The flowers are unisexual. The male flowers have four lobed, minute, granular,
dotted, greenish calyx, they have eight stamens. The female flowers have three
triangular-ovate, ciliate sepal and superior ovary. The fruit is a 3-lobbed capsule
splitting into three cocci, each 2-valved and 1-seeded (Schmelzer and Gurib-Fakim
2008).
Acalypha indica occurs in Nigeria and from Sudan eastward to Somalia and
Southwards through Democratic Republic of Congo and East Africa and up to
South Africa. Also found in Indian Ocean Islands, India, South East Asia and
Oceania (Schmelzer and Gurib-Fakim 2008).
Acalypha indica thrives on sandy margins of rivers and seasonal water courses, usu-
ally in the shade of thickets. It also grows on rocky hillside and as a weed on fields
from sea level up to 135 m altitude. Acalypha indica flowers throughout the year in
regions without a pronounced dry season (Schmelzer and Gurib-Fakim 2008).
Acalypha indica is reported to contain alkaloids, tannins, saponins, steroids and
proteins (Rajaselvam et al. 2012). The dried aerial parts contain a cyanogenic
glycoside, acalyphin, which is a 3-cyanopyridone derivative. Flavonoids, such as
the kaempferol glycosides mauritianin, clitorin, nicotiflorin, and biorobin have been
isolated from the flowers and the leaves. The plant also contains tannins, β-sitosterol,
acalyphamide, aurantiamide, succinimide and the pyraquinolinone alkaloid
flindersin.
Acalypha indica is used as a diuretic, antihelmintic and for respiratory problems
such as bronchitis, asthma and pneumonia (Rajaselvam et al. 2012).
The main parts that are usually exploited in medicine and the roots, leaf, stalk
and flowers they are used as emetic, expectorant, laxative and diuretic. The plant is
useful in bronchitis pneumonia and pulmonary tuberculosis. It also has anti-diabetic
properties (Ishak et al. 2013).
In East Africa, the leaf sap is used to treat eye infections, while the leave powder
is applied to maggot infested wounds. It is used in Comoros to treat joint pains and
a root decoction is used in the Seychelles to treat stomach ache and intestinal
wounds. In Madagascar, the crushed aerial parts are applied to skin parasite and an
infusion is taken as a purgative and vermifuge, while in Mauritius, the juice of the
crushed leaves mixed with salt or a decoction of the aerial parts is applied to scabies
and other skin problems (Schmelzer and Gurib-Fakim 2008).
Antimicrobial studies of the aqueous and acetone extracts of Acalypha indica shared
that the extracts inhibited the growth of Escherichia coli, Klepsiella sp,
Staphylococcus aureous and Bacillus Subtilis (Rajaselvam et al. 2012). Ishak et al.
(2013) also reported that the petroleum ether, chloroform and methanol extracts of
fresh parts of Acalypha indica exhibited antibacterial effect on Staphylococcus

aureous and antifungal effect on Candida albicans.
Cytotoxic activity against Hela cell lines has also been reported while and
ethanol leaf extract of the plant showed significant inhibition to Viper russelli
venom-induced lethality, hemmorhage, necrotizing and mast cell degranulation in
rats. It also has cardiotoxic and neurotoxic effects on isolated frog tissue (Schmelzer
and Gurib-Fakim 2008).
Acalypha indica was formerly listed in the British Pharmacopoeia, and because
of its numerical uses in India, it is listed in the pharmacopoeia of India (Schmelzer
and Gurib-Fakim 2008).
It has been reported to be useful in treating pneumoniae, asthma, rheumatism and
several other ailments. The dried leaves of Acalypha indica was made into a poul-
tice to treat bedsores and wounds and the juice of Acalypha indica is added to oil or
lime and used to treat a variety of skin disorders (Jagatheeswari et al. 2013). It is
also used as a purgative, a very good remedy in the treatment of piles, and the root
is used as a dewormer in children when administered in the morning empty stom-
ach. When the leaves are used with turmeric, it is useful in relief from acne and
pimples (Kucinggalak 2015).
Euphorbia hirta Linn (Synonyms: Chaemescyle hirta)
Common Names Asthma herb (English), Odane Inenemili (Ibo), Kinkerechintara,
Lupka (Nupe), Nonon kurciya, Janyaro (Hausa), Emile/Egele (Yoruba) (Picture 9)
Euphorbia hirta is a plant belonging to the family: ‘Euphorbiaceae’. It is a branched
annual herb, prostrate to ascending, with branches up to 50 cm long, with latex; all
parts short-hairy and with sparse yellow hairs c. 1.5 mm long. Leaves opposite,
distichous, simple; stipules linear, up to 2.5 mm long; petiole up to 3.5 mm long;
blade ovate, 1–4 cm × 0.5–2 cm, base very unequal, one side cuneate, the other side
rounded, apex almost acute, margin finely toothed, often with a purple blotch near
the midvein. Inflorescence a terminal or axillary cluster of flowers, called a
‘cyathium’, with several cyathia densely clustered into a cyme c. 15 mm in diame-
ter; peduncle up to 15(–20) mm long; cyathia with a cup-shaped involucre c. 1 mm
in diameter, tinged purple, lobes triangular, fringed, glands 4, tiny, elliptical, green
or purplish, with minute white to pink appendages, each involucre containing 1
female flower surrounded by many male flowers. Flowers unisexual; male flowers
sessile, bracteoles linear, fringed, perianth absent, stamen 1, c. 1 mm long; female
flowers with short pedicel, perianth a rim, ovary superior, short-hairy, 3-celled,
styles 3, minute, apex 2-fid. Fruit a just exserted, acutely 3-lobed capsule c. 1 mm
in diameter, base truncate, short-hairy, 3-seeded. Seeds oblong-conical, c. 1 mm
long, slightly wrinkled, pinkish brown, without caruncle (Johnson et al. 1999).
Picture 9 Euphorbia hirta

(Source: Authors)
Euphorbia hirta is said to be native to Central America, but a very common weed of
the tropics and subtropics; it occurs throughout tropical Africa and also in South
Africa.
Euphorbia hirta grows in cultivated fields, gardens, roadsides and waste places,
from sea-level up to 2000 m altitude.Euphorbia hirta is considered a weed, and can
be a nuisance in crops due to the large number of seedlings.
Important constituents of the aerial parts are terpenoids, including triterpenes:
α-amyrin, β-amyrin, friedelin, taraxerol, and esters of it: taraxerone, 11α,
12α-oxidotaraxerol, cycloartenol, 24-methylene-cycloartenol, and euphorbol hexa-
cosoate. The aerial parts and roots also contain diterpene esters of the phorbol type
and ingenol type, including 12-deoxyphorbol-13-dodecanoate-20-acetate,
12-deoxyphorbol-13-phenylacetate-20-acetate, ingenol triacetate, as well as the
highly toxic tinyatoxin, a resiniferonol derivative. Other terpenoids isolated are ste-
rols including β-sitosterol, campesterol, cholesterol and stigmasterol (Abu-Sayeed
et al. 2005).
Tannins isolated from the plant include the dimeric hydrolysable dehydroellagi-
tannins euphorbins A, B, C, E and terchebin, the monomeric hydrolysable tannins
geraniin, 2,4,6-tri-O-galloyl-β-D-glucose and 1,2,3,4,6-penta-O-galloyl-β-D-
glucose and the esters 5-O-caffeoylquinic acid (neochlorogenic acid) and 3,4-di-O-
galloylquinic acid, and benzyl gallate (Adedapo et al. 2005)
Acids isolated include ellagic acid, gallic acid, tannic acid, maleic acid and tar-
taric acid (Adedapo et al. 2005). Flavonoids isolated include quercetin, quercitrin,
quercitol and derivatives containing rhamnose, quercetin-rhamoside, a chlorophe-
nolic acid, rutin, leucocyanidin, leucocyanidol, myricitrin, cyanidin 3,5-diglucoside,
pelargonium 3,5-diglucoside and camphol. The flavonol glycoside xanthorhamnin
was also isolated. The stems contain the hydrocarbon hentriacontane and myricyl
alcohol. The latex contains inositol, taraxerol, friedelin, β-sitosterol, ellagic acid,
kaempferol, quercitol and quercitrin (Abu-Sayeed et al. 2005).
The mineral content of a sample of the dried leaves was: Ca 1.1%, P 0.3%, Fe
0.03%, Mg 0.5%, Mn 0.01%, Zn 0.01% and Cu 0.002%. Fresh leaves from
Euphorbia hirta plants of Nigerian origin were found to contain high levels of Mn
(189 ppm), Cu (30.5 ppm), Zn (152 ppm), and NO3 (4600 ppm). Varying propor-
tions of Fe, Mg, K, Ca and Na were found (Wallace et al. 1990).
Medicine
Euphorbia hirta is an important medicinal herb used throughout its distribution
area, including tropical Africa. It is held in high esteem, as a decoction or infusion,
to treat gastrointestinal disorders, including intestinal parasites, diarrhoea, peptic
ulcers, heartburn, vomiting and amoebic dysentery (Oyewale et al. 2002).
It is also regarded as an outstanding medication to treat respiratory system disor-
ders, including asthma, bronchitis, hay fever, laryngeal spasms, emphysema, coughs
and colds. The leaves are mixed with those of Datura metel L. in preparing ‘asthma
cigarettes (Hienmann and Bucar 1994).
Other principal uses are as a diuretic to treat uro-genital diseases, such as kidney
stones, menstrual problems, sterility and venereal diseases. The plant is also used to
treat affections of the skin and mucous membranes, including warts, scabies, tinea,
thrush, aphthae, fungal afflictions, measles, guinea-worm and as an antiseptic to
treat wounds, sores and conjunctivitis (Edwin et al. 2007).
The plant has a reputation as an analgesic to treat severe headache, toothache,
rheumatism, colic and pains during pregnancy. It is used as an antidote and pain
relief of scorpion stings and snakebites. It is antipyretic and anti-inflammatory
(Ogbulie et al. 2007).
It is also used in the treatment of jaundice, hypertension, oedema, anaemia and
malaria, as an aphrodisiac, and to facilitate childbirth. In West Africa the plants are
widely used as a galactagogue, and in Nigeria they are marketed for this purpose. In
Uganda whole plants are chewed to induce labour during childbirth (Hienmann and
Bucar 1994).
Several of the traditional medicinal uses of Euphorbia hirta have been supported by
in-vitro studies. Ethanol, petroleum ether and dichloromethane extracts of whole
plants showed significant in-vitro antiplasmodial activity (IC50 = 3 μg/ml) and
decreased growth of Plasmodium falciparum by 89–100% at a test concentration of
6 μg/ml (Tona et al. 2004). In vivo, the extracts reduced parasitaemia in mice
infected with Plasmodium berghei berghei at oral doses of 100–400 mg/kg per day.
From a methanolic extract of the aerial parts the flavonol glycosides afzelin,
quercitrin and myricitrin were isolated, which showed proliferation inhibition of
Plasmodium falciparum, with IC50 values of 1.1, 4.1, 5.4 μg/ml respectively, while
they exhibited little cytotoxic effect against human epidermoid carcinoma KB 3–1
cells.
An ethanolic extract was active in selectively inhibiting Herpes simplex virus
type-1 (0.001–0.1 mg/ml) (Somchit et al. 2001). Lyophilized aqueous extract of the
aerial parts has been evaluated for analgesic, antipyretic and anti-inflammatory
properties in mice and rats. The extract exerted central analgesic properties at doses
of 20 and 25 mg/kg, and antipyretic activity at doses of 100 and 400 mg/kg, whereas
anti-inflammatory effects against carrageenan-induced oedema in rats were
observed at a dose of 100 mg/kg (Ogbulie et al. 2007).
The aqueous extract of the aerial parts has been found to strongly reduce the
release of prostaglandins, and thus depress inflammation (Hienmann and Bucar
1994). An ethanolic extract of the aerial parts was found to possess a prominent
anti-anaphylactic activity and also showed significant antihistaminic, anti-
inflammatory and immunosuppressive properties in various animal models. Water
and ethanolic leaf extracts produced a time-dependent increase in urine output in
rats. A methanol extract of leaves and stems inhibited the activity of angiotensin-
converting enzyme by 90% at 500 μg and 50% at 160 μg. The extract (10 mg/100 g,
intraperitoneally) significantly decreased the amount of water consumed by rats. An
ethanolic extract of the whole plant showed a dose-dependent ulcer protective effect
in rats (Rao et al. 2003). The active compound was found to be quercetin, which had
an anti-ulcer activity ranging from 48–64% comparable to 61–80% of the standard
drug ranitidine. An ethanolic extract of the aerial parts showed significant
hepatoprotective activity in rats. Extracts of whole plant material have oestrogenic
activity in female guinea pigs, when given orally. In organ bath tests with ileum
preparations, shikimic acid and choline extracted from the aerial parts had relaxing
and contracting properties, respectively (Lanhers et al. 1990). Shikimic acid also
has acute toxicity, mutagenicity and carcinogenicity. The aqueous crude extract sig-
nificantly reduced the faecal egg count of helminths in dogs (Johnson et al. 1999).
Several of the extracts of Euphorbia hirta showed potential for controlling plant
diseases and pests (Hienmann and Bucar 1994). For example, a whole plant extract
inhibited growth of vascular wilt (Fusarium oxysporum) and the causal agent of
sheath rot of rice, Sarocladium oryzae; aqueous extracts of the aerial parts inhibited
aflatoxin production by Aspergillus parasiticus on agricultural crops, including rice,
wheat, maize and groundnuts. Leaf extracts completely inhibited soft rot infection
caused by the bacteria Erwinia carotovora pv. carotovora. The infectivity of tobacco
mosaic virus on Nicotiana glutinosa L. was strongly inhibited (>80%) by tannins
extracted from the aerial parts. The latex inhibited sugarcane mosaic virus-A by
78.5% and sugarcane mosaic virus-F by 80%. Root and leaf extracts showed nema-
ticidal activity against Meloidogyne incognita; a whole plant extract effectively
reduced hatching in the nematode Heterodera avenae. A 10% ethanol crude extract
showed significant larvicidal action against the larvae of the tick Boophilus microp-
lus. Aqueous stem, latex and leaf extracts have potent molluscicidal activity against
the freshwater snails Lymnaea acuminata and Indoplanorbis exustus, both interme-
diate hosts of Fasciola hepatica and Fasciola gigantica, which cause endemic fas-
cioliasis in cattle and livestock (Singh and Khandelwal 2010). Toxicity of the
extracts was time dependent and dose dependent against both snails. The doses that
can be used for killing 90% of the Lymnaea acuminata populations are safe for the
fish Channa punctatus.
An aqueous extract of the whole plant acts as an antidiarrhoeic agent by anti-
amoebic, antibacterial and antisplasmodic activities. The antidiarrhoeal activity is
attributed to quercitrin through the release of the aglycone quercetin in the intestine
(Singh and Khandelwal 2010). Quercitrin showed antidiarrhoeic activity at doses of
50 mg/kg in mice. A crude plant extract and an ethanolic extract had significant anti-
amoebic activity against Entamoeba histolytica in vitro at 35 mg/ml. An aqueous
lyophilysate of the whole plant showed higher activity against Entamoeba histolyt-
ica than either the ethyl acetate or methanol extracts, at 30 mg/ml. An aqueous plant
extract showed concentration-related activity against non-pathogenic amoebae of
the Amoeba proteus type. Different extracts from the aerial parts showed antibacte-
rial activity against a wide spectrum of both gram-positive and gram-negative bac-
teria. Extracts of the aerial parts showed strong antibacterial activity against Shigella
dysenteriae, a causal agent for dysentery in humans. The active compound was
found to be ethyl gallate, which has broad spectrum antibiotic activity at non-toxic
doses. A crude ethanol extract of the whole plant showed dose-dependent activity
against Candida albicans, but not against several other pathogenic fungi. Some of
the isolated antibacterial compounds were taraxerone and 11α, 12α-oxidotaraxerol,
which showed low cytotoxicity.
The levels of chemicals in Euphorbia hirta are high enough to constitute a source
of toxicosis to animals consuming the plants and should also be a source of concern
in medicinal use. Few toxic effects have been documented for Euphorbia hirta. An
ether extract was found to be toxic in a brine shrimp lethality test, whereas ethyl
acetate and aqueous extracts were within safe limits (Ogbulie et al. 2007). In another
test, however, an aqueous crude extract was found to cause testicular degeneration
in sexually mature male rats as well as a reduction in the mean seminiferous tubular
diameter. Several other extracts given orally to rats caused dullness and anorexia
and induced a 20% mortality rate. Some fractions from the ethanolic extract showed
potentially deleterious effects on the blood serum chemistry of rats (Adedapo et al.
2005). In feeding experiments with rats however, no difference in the blood serum
was found after a prolonged period of adding Euphorbia hirta to the diet. It was also
found that drying Euphorbia hirta prior to extraction considerably reduces the cyto-
toxic activity of certain of its extracts.
Euphorbia unispina N.E. Br
Common and Local Names Candle plant (English) (Newton 2008) and Oro Adete
in Yoruba language (Picture 10).
Picture 10 Euphorbia unispina (Source: Authors)
Euphorbia unispina is a monoecious, sparsely branching shrub that can attain 3.5 m
tall. The branches are cylindrical, up to 2.5 cm in diameter. It is silvery-grey in
colour and covered with shallow tubercles and horny spine. The leaves are simple
and arranged spirally at the stem apex in 4–5 ranks, the stipules are modified into
two stout spines, 6–10 mm long. The petioles are short and thick, and blades are
oblong to spoon-shaped, 5–12 cm × 1.5–5 cm. The base is long-cuneate, apex
notched and fringed, acute or rounded, almost entire, fleshy, glabrous, pinnately
veined. The inflorescence is an axillary cyme at the ends of branches, consisting of
clusters of flowers, each cluster called a cyathium. The flowers are red, unisexual,
male flowers sessile, perianth absent, stamen shortly exserted. Female flowers are
with curved pedicel, 4–8 mm long in fruit, perianth 5-lobed, ovary superior, 3-celled,
glabrous, styles 3, up to 2 mm long, slender, fused at base, bifid at apex. The fruit is
an obtusely 3-lobed capsule, about 6 mm in diameter, glabrous and three-seeded.
The seeds are ovoid in shape (Newton 2008).
Euphorbia unispina is found in Africa from Guinea and Mali east to southern Sudan.
Euphorbia unispina grows on rocky hills and slopes in savanna (Newton 2008)
Chemical Constituents
The latex of Euphorbia unispina contains esters of diterpene alcohols of the tigliane
type, 12-deoxyphorbol and 12-deoxy-16-hydroxyphorbol, and the daphnane type,
resiniferonol, as well as several macrocyclic esters of the diterpene alcohol
18-hydroxyingol (Rao et al. 2003).
Uses
The latex of Euphorbia unispina is applied to the neck to cure sleeping sickness in
the West African countries of Guinea, Mali and Cote d’Ivoire because it is believed
that the disease is caused by ganglia in the neck. In Cote d’Ivoire and Nigeria, the
latex is applied to leprosy sores. An inhalation of the stem ash is used to treat asthma
in Benin, while a mixture of palm oil with the latex is taken to treat constipation and
colic. Skin diseases and hemmorroids are treated by a macerate of the stem in water
while in Northern Nigeria, the latex is rubbed onto the body to treat mental illness.
The latex is also use in dental care by dropping it on carious tooth to relieve tooth-
ache or to help loosen the tooth and render extraction easier. Smoking of the dried
leaves in a pipe is used to treat bronchitis. In a mixture with other ingredients such
as Strophanthus species, the latex is used in the preparation of arrow poison (Newton
2008). The root of Euphorbia unispina is also used to treat cancer (Soladoye et al.
2010).
Euphorbia unispina latex is very caustic and toxic. It is very irritating to the skin
and mucous membranes. It can cause blindness when in contact with the eyes. In
Northern Nigeria, the latex is used as a poison to commit murder and suicide
(Newton 2008).
Phyllanthus muellerianus (Kuntze) Exell (Pictures 11 and 12)

Phyllantus muellerianus belong to the family Euphorbiaceae.
Morphology
It is a monoecious, glabrous, straggling or climbing shrub or small tree up to 12 m
tall; branches spreading or pendulous, main branches stout, angular, reddish tinged,
branchlets 15–20(–25) cm long, with several short axillary shoots; branch basis
transformed into a pair of spines c. 4 mm long, purplish brown. Leaves alternate,
distichous along lateral twigs, simple, glabrous; stipules lanceolate, ca. 2 mm long,
Pictures 11 and 12 Phyllantus muellerianus (Source: faunaandfloraofvietnam.blogspot.

com,www.prota4u.org)
acuminate; petiole 3–5 mm long; blade ovate, elliptical-ovate to ovate-lanceolate,

3–9 cm × 2–4.5 cm, base cuneate to rounded, apex acute to obtuse, with 10–14 pairs
of lateral veins. Inflorescence a false raceme on short axillary shoots, 2–6 cm long,
solitary or several together, with flowers in clusters having 2–3 male flowers and 1
female flower in each cluster. Flowers unisexual; perianth lobes 5, elliptical, c.
1 mm long, rounded, greenish white or greenish yellow; male flowers with pedicel
c. 1.5 mm long, disk glands 5, free, minutely warted, fleshy, stamens 5, free, unequal,
anthers very small; female flowers with stout pedicel c. 1 mm long, disk glands 5,
free or fused, knobbly, fleshy, ovary superior, ellipsoid, warty, 4–5-celled, styles
4–5, free, c. 0.5 mm long, 2-fid at apex. Fruit a fleshy, nearly globose capsule
3–4 mm in diameter, usually smooth, green, becoming red, later black, 6-seeded.
Seeds angular, c. 1 mm long, with faint ridges, bright reddish brown or yellowish
brown.
Phyllanthus muellerianus occurs from Senegal and Guinea Bissau east to Sudan
and Kenya and south to northern Angola and northern Mozambique. It is a large
genus comprising about 750 species in tropical and subtropical regions, with about
150 species in mainland tropical Africa and about 60 in Madagascar and the Indian
Ocean islands (Burkill 1994).
Phyllanthus muellerianus occurs in riverine forest and wooded grassland, on deep
and well-drained soils, from sea-level up to 1600 m altitude. In Nigeria, Phyllanthus
muellerianus is reported as a weed of rice fields. Phyllanthus muellerianus can be
propagated through seeds and stem cuttings (Arbonnier 2004).
From the extracted essential oils of the leves was found to be (E)-isoelemicin
(Brusto et al. 2012). Preliminary phytochemical screening of the leaves and stem
methnolic extracts showed that both the extracts contained tannins, flavonoids,
saponins, alkaloids, and anthraquinones. From the stem bark the triterpenoids
22-β-hydroxyfriedel-ene and 1β,22β-dihydroxyfriedelin were isolated.
Medicine
Phyllanthus muellerianus is widely used to treat intestinal troubles. An infusion of
the young shoots is taken to treat severe dysentery. In Sierra Leone a leaf decoction
is taken to treat constipation. In Ghana and Nigeria cooked roots, sometimes with
maize meal or other plants, are taken to treat severe dysentery. In Congo powdered
roasted roots with palm oil are taken to treat stomach problems and as an anti-
emetic. In Tanzania roots are pounded in water and the liquid is drunk to treat diar-
rhoea. Boiled roots are also applied as enema to treat stomach-ache.
In West Africa leaf sap or sap from the thick hollow stem is applied as eye drops
to treat pain in the eyes, eye infections or to remove a foreign body. In Côte d’Ivoire
and Burkina Faso twigs are sucked to prevent toothache. Powdered roots are used as
a snuff and a bark decoction is taken to treat a sore throat, cough, pneumonia and
enlarged glands. Pulped leafy twigs are rubbed on the body to treat paralysis. In
Nigeria a root bark decoction is taken as an alternative to treat fever. A twig and root
decoction is taken to treat jaundice and urethral discharges. In the Central African
Republic fresh root bark is crushed and macerated in water or palm wine, and the
liquid drunk as an aphrodisiac. In Gabon roasted powdered twigs are eaten with
plant ash to treat dysmenorrhoea. In DR Congo dried bark powder is sniffed to treat
colds and sinusitis. A root bark decoction is applied to swellings and is drunk to
treat gonorrhea. Stem ash is applied to scarifications to treat rheumatism and inter-
costal pain. In Tanzania a root decoction is taken to treat hard abscesses; powdered
dried roots and stem bark are sprinkled on wounds as a dressing.
Throughout West Africa pounded leaves are applied as wound dressing. In Côte
d’Ivoire the leaves are eaten, together with young leaves of Funtumia elastica
(Preuss) Stapf, to improve male fertility. In Ghana and Nigeria leaves boiled with
palm fruit are given to women after delivery as a general tonic. In Cameroon a
maceration of the leaves and roots is used to wash the body to treat rash with fever
in children. In DR Congo a leaf decoction is taken to treat anaemia and also used
as a mouthwash to treat toothache. A leaf extract is used as a bath and a vapour bath
to treat venereal diseases. Cooked leaves are applied to the gums to treat toothache.
A flower infusion is cooling and gently aperient. The fruits are edible and slightly
acidic. In Sierra Leone and Nigeria the sap from the hollow branches is considered
potable.
In Cameroon the bark is sometimes added to palm wine to render it strongly
intoxicating. In Kenya the stems are considered excellent firewood; branches thicker
than 15 cm become hollow and are less used. In East Africa the brown dye from the
bark is used to dye mats and fishing lines. From the whole plant a black dye is
obtained used to colour fibres. In Zambia the wood is used for rafters and other
construction work. It is also used to make fish traps and basketry. The leaves are
used as fodder. In Sierra Leone and Nigeria leaves are sometimes cooked with food
or in soup as a seasoning. In Nigeria twigs are used as chew-sticks after removal of
the spines. Fruit pulp is used as a hair fixative. In Gabon Phyllanthus muellerianus
is used in magic to lift taboos.
Phytochemical screening of the leaves and stem bark showed the presence of tan-
nins, flavonoids, saponins, alkaloids and anthraquinones. A leaf extract showed
moderate antiplasmodial activity (IC50 = 9.4 μg/ml) and low cytotoxicity on mam-
malian cell lines. Both the aqueous and methanol extracts of the leaves and stem
bark showed high antibacterial activity against Staphylococcus aureus and
Pseudomonas aeruginosa. The activity of the extracts was relatively stable at high
temperatures and was enhanced at low pH. In another test a chloroform extract
showed high antifungal activity against Candida albicans and antibacterial activity
against Escherichia coli. A crude aqueous extract orally administered to rats caused
significant changes in haematological and biochemical parameters, which are used
as indices of toxicity (Anuka et al. 2005).
Fabaceae
Senna occidentalis Linn (Synonyms: Cassia occidentalis L., Ditremexa occiden-

talis (L.) Britt. & Rose. Senna occidentalis (L.) Roxb.; Senna occidentalis var.
sophera (L.) (Pictures 13 and 14)
Senna occidentalisis of the family- Fabaceae (Leguminosae) and sub-family
Caesalpinioideae, is commonly called stink weed (Saidu et al. 2011).
Common Names
Coffee senna, ant bush, antbush, arsenic bush, negro coffee, Nigerian senna, septic-
weed, sickle pod, stink weed, stinking pea, stinking weed, stinkingweed, stinkweed,
styptic weed.
Senna occidentalis is an erect herb commonly found by the road sides in ditches and
waste dumping sites (Saidu et al. 2011). The leaves are alternate, compound and
pinnate, consisting of four or five pairs of leaflets widely spaced along a common
stalk. The leaflets are pointed at the tips and the flowers are yellow and produced in
loose clusters in the terminal leaf axils. The fruits are in form of thin pods, 3–4 in
long and pale green when tender, thick and dark green when mature. It brings out
pods from September to November and the pods are slightly curved, with paler
longitudinal stripes along the edges. Each pod contains 50–60 small seeds together
weighing 1.90 to 2.25 g. From December onwards, the pods start drying up, turn
brown and the seeds turn dark brown (Vashishtha et al. 2009).
Senna occidentalis is found across West Africa (Saidu et al. 2011).
Senna occidentalis is a weed found in degraded pastures and plantations, which is
poisonous to cattle and other domestic animals (Modena et al. 2012). It was
shown experimentally that Senna occidentalis can survive under widely variant
Pictures 13 and 14 Senna occidentalisis (Source: Anand Kumar Reddy, http://pinpicsnow.com)
light intensities. Although it occurs at optimal temperature of 25.0 °C, it also

survives at its extreme temperatures of 10.0–12.5 °C (low) and 45 °C (high).
Optimal pH is 6 although it relatively prefers more acidic condition than basic.
The sandy loam soil is better preferred by the plant.
Phytochemical screening of aqueous extracts of Senna occidentalis revealed the
presence of flavonoids, cardenolides, saponins, anthraquinones and alkaloids (Saidu
et al. 2011). The hexane, ethyl acetate and methanol extracts revealed the presence
of tannins, alkaloids, reducing sugar, phenols, anthraquinones, resins, saponins and
glycosides (Odeja et al. 2014). Senna occidentalis has also been reported to contain
carbohydrates, saponins, sterols, flavonoids, resins, alkaloids, terpenes, anthraqui-
nones, glycosides and balsam, which are indicators to its medicinal attributes.
Flavonoids and resins might be responsible for its anti-inflammatory properties;
alkaloids known for decreasing blood pressure, balancing the nervous system in
case of mental illness and antimalarial properties. Tannins help in wound healing
and anti-parasitic properties. Tannins possess antitumor and antviral properties and
eudesmane sesquiterpenes possess antibacterial properties (Vijayalakshmi et al.
2013). Other compounds reported include 1,8-dihydroxyl-2-methyl anthraquinone,
1,4,5-trihydroxyl-3-methyl-7-methoxy anthraquinone, cassiaoccidentalin A,B and
C which are C-glycosides, achrosine, anthrones, apigenin, aurantiobtusin, campes-
terol, cassiollin, chrysotusin, chrysophanic acid, chrysarobin, chrysoeviol, essential
oils, funicolosin, galactopyranosyl, helminthosporin, islandicin, kaempferol, ligno-
ceric acid, linoleic acid, linolenic acid, mannitol, mannopyranosyl, matteucinol,
obtusifolin, oleic acid, physcion, quercetin, rhamnosides, rhein, rubrofusarin, sitos-
terols and xanthorin (Vijayalakshmi et al. 2013).
Senna occidentalis is effective in the treatment of dysentery and diarrhea which are
mainly caused by microorganisms. An infusion of the leaf is also used to treat
typhoid fever (Saidu et al. 2011). The leaves and roots are used as remedy for bacte-
rial and fungal infections and can also boost immune function. The leaves and roots
are ingredients of tonics used to treat liver disorders, abscesses, insect bites, scor-
pion sting, constipation, diabetes, oedema, fever, inflammation, itch, rheumatism,
ringworm, scabies, skin diseases, snakebite and wounds (Vashishtha et al. 2009)
The pods and seeds are considered toxic to grazing animals that feed on them as
they become seriously ill and may die (Vashishtha et al. 2009)
The hexane, ethylacetate and methanol extracts of Senna occidentalis at varying
concentrations inhibited the growth of Staphilococcus aureus, Escherichia coli,
Bacillus subtilis, Pseudomonas aeruginosa, Salmonella typhi, Klebsiella pneumo-
nia, Candida albicans, Aspergillus niger, Penicillium notatum and Rhizopus stoloni-
fer to varying extent (Odeja et al. 2014). The methanol, aqueous, benzene, petroleum
ether and chloroform extract of Cassia occidentalis leaf were screened for
antibacterial activity. The methanol extract inhibited the growth of P. aeruginosa,
K. pneumonia, P. mirabilis, E. coli, S. aureus and S. epidermidis. The aqueous

extract was effective against P. vulgaris, K pneumonia, and P. aeruginosa while the
benzene and petroleum ether extracts were effective against P. mirabilis and E. coli.
The chloroform extract was ineffective against all the test organisms (Arya et al.
2010). The extract of Senna occidentalis flower showed maximum growth inhibi-
tion against Klebsiella pneumonia but no activity against Staphylococcus aureus,
Streptococcus pneumonia and Pseudomonas aeruginosa (Daniyan et al. 2011). The
crude leaf extract also inhibited the growth of Pseudomonas aeruginosa,
Staphylococcus aureus, Escherichia coli, Bacillus subtillis and Candida albicans.
Senna occidentalis also possess antioxidant, hepatoprotective, anti-inflammatory,
larvicidal, antimalarial, antidiabetic, analgesic, antipyretic and antidepressant prop-
erties (Vijayalakshmi et al. 2013).
Senna occidentalis is used as a broad spectrum internal and external antimicrobial
to treat bacterial and fungal infections. Also used for liver disorders (jaundice, lepa-
titis, cirrhosis, detoxification, injury and failure, bile stimulant, etc) and as treatment
for intestinal worms, internal parasites, skin parasites. Moreso, used as a cellular
protector and preventative to cell damage, viz: immune, liver, kidney and cancer
(Sadiq et al. 2012).
Malvaceae
Grewia mollis Juss (Synonyms: Grewia venusta Fresen and Grewia pubescens
P.Beauv) (Picture 15)
Picture 15 Grewia mollis (Source: Author)

Grewia mollis is a shrub or small tree up to 20 ft tall, of Malvaceae family (previ-
ously belonging toTiliacaea family) widely distributed within the Northern, middle
belt of Nigeria and some African countries. Grewia mollis is often gregarious, with
branch-suckering leading to the formation of thickets various parts of the plant are
used in food and medicine (Asuku et al. 2012). It is a shrub or small tree up to
10.5 m tall, often multi-stemmed; stem diameter up to 30 cm; young branches
densely stellate-pubescent, turning dark grey to purple with age; outer bark black,
thick, rough, flaking and deeply fissured, inner bark yellowish to brown, fibrous.
Leaves alternate, simple; stipules lanceolate, 5–10 mm long, slightly hairy, cadu-
cous; petiole 4–13 mm long, greyish to reddish brown pubescent; blade elliptical to
elliptical-oblong. Inflorescence is a cyme. Flowers bisexual, regular, 5-merous,
slightly scented; pedicel 3–11 mm long; sepals linear-oblong, 6–11 mm long, grey-
ish hairy outside; petals obovate to oblong, 4–6(–8) mm × c. 2 mm, sometimes
notched at the apex, bright yellow; apex densely hairy; stamens numerous, 3–6 mm
long; ovary superior, 1.5–2 mm long, densely hairy. Fruit a globose drupe 4–8 mm
× 5–8 mm, finely whitish hairy, yellow turning black; endocarp hard and woody.
Grewia mollis occurs widely in tropical Africa, from Senegal and Nigeria eastward
to Somalia and southward to Zambia and Zimbabwe.
Habitat and Ecology
Grewia mollis occurs in tropical climates with annual average rainfall of 600–
1400 mm. It is usually distributed in lowlands of West Africa up to high altitude
zones in East Africa and thrives in forest, open woodland, riverine and seasonally
flooded grassland. It is known to be highly resistant to fire.
Collection Practices
Grewia mollis is distributed in the tropical and subtropical parts of Africa and Asia
.Germination of Grewia mollis often occurs after a bush fire followed by rains but
growth is slow. Flowering is around the end of March and October depending on
locale. The parts of Grewia mollis are normally collected from the wild but it can be
propagated by seed or seedlings. Seeds are usually collected from dried fruits fallen
on the ground.
Hanan and co-workers (2012) have investigated extensively the plant Grewa mollis
and isolated the following compounds, 7-(1-O-β-D-galacto turoniole-4-(1-O-β-
glucopyranesyl-3,4,5,7 tetrahydroxyflavone, lutelion, 7-β-hydroxy-2,3-en-
deoxojessic acid, 7-β- hydroxy-2,3-deoxojessic acid sitesterol and β-sitosterol-
3-O-glucoside,from the aerial part of the plant. Further phytochemical investigation
on biologically active methanolic extract resulted in the isolation of luteloin,
7-(1-O-β-D-galacturonide)-4′-(1-O-β-glucopyranosyl)-3′4′,5,7-tetrahydroxyflavone,
7-β-hydroxy-23 enedeoxojessic acid , 7-β-hydroxy-23- deoxojessic acid ,
β-sitosterol and β-sitosterol-3-O-glucoside. Two other compounds; triterpene
lup-20-en-2-ol and 1,3-hexyloxacyclotridec- 10-en-2-one, have been isolated from
the roots of the plant (Efiom and Oku 2012).

In Nigeria the fruit of Grewia mollis is used as a febrifuge. The stem bark, branch,
trunk, decoction is used to cure constipation. The mucilaginous bark and leaves are
applied to ulcers, cuts, sores and snakebies and acclaimed to have laxative property.
Bark and root preparations are taken to treat cough. Extracts of bark and leaves are
reportedly drunk to treat fever. In Togo a decoction of the stem bark is drunk to treat
diarrhoea, and a macerate is taken to ease childbirth. An infusion of the bark is used
to treat colic dysentery (Adamu et al. 2005). In East Africa leaves pounded and
mixed with water are taken against stomach problems and also given to constipated
domestic animals. In Côte d’Ivoire a decoction of the leaves is used in baths and
drinks to cure rickets in children. A decoction of the roots is drunk in Senegal in
case of palpitation. In Central Africa sap from root-shavings is placed under the
eyelid to treat sore eyes, whereas a liquid obtained by kneading the root bark in
water is drunk to treat stomach-ache, colic and poisoning by certain plants. In Ghana
a paste of ground roots is applied to rheumatic swellings and inflammation (Bekalo
et al. 2009; Asuku et al. 2012).
Biological and Cytotoxic Activities
The invitro antibacterial activity of the aerial part of Grewia mollis was investigated
by Hanan and co-workers (2012) against various strains of bacteria such as
Staphylococcus epidermidis, Bacillus subtilis, Staphylococcus aureus, Klebsiella
pneumoniae, Pseudomonas aeruginosa and Escherichia coli. The methanolic
extract showed significant activity against Staphylococcus epidermidis, Bacillus
subtilis and Staphylococcus aureus when compared with the standard antibiotics.
Methanolic extract of G. mollis aerial part showed inhibitory activity against oral
cavity pathogen with extract exhibiting a concentration-dependent killing of
Staphylococcus aureus and also preventing the formation of water-insoluble glucan.
This researchers further demonstrated the antiinflammatory and antihypertensive
activities of the aerial part of G.mollis. The antioxidant an activity of the leaves
extract of the plant has also been reported (Asuku et al. 2012). The researchers fur-
ther demonstrated the protective and ameliorative potentials of this plant against
experimentally induced liver injuries in rats.
2 Conclusion
The properties, uses and application of some wild herbaceous plants distributed
throughout Nigeria have been reviewed. The active compounds variously isolated as
well as their pharmacognostic and biological properties of the plants reviewed have
been highlighted. Since many of the biological activities of these plants have been
validated experimentally, further studies on the potential industrial use of these
plants in developing countries like Nigeria should be seriously explored, such as in
drugs development, as well as production of natural supplements, nutraceuticals,
natural insecticide, phytochemicals for industrial usage etc. The cultivation of wild
but useful plants could be an essential step that can bring about the much needed
turn-around in the nations’ agricultural system, economy and conservation of plant
biodiversity in Nigeria.
Acknowledgments Professor S.E. Atawodi’s research work on antioxidants was partly supported

by Alexander von Humboldt Foundation of Germany and the German Cancer Research Centre
(DKFZ), Heidelberg.
References
Abu-Sayeed M, Ali MA, Bhattacharjee PK, Islam A, Astaq GRM, Khan M, Yeasmin S (2005)
Biological evaluation of extracts and triterpenoids of Euphorbia hirta. Pakistan J Sci Ind Res
48(2):122–125
Adamu HM, Abayeh OJ, Agho MO, Abdullahi AL, Uba A, Dukku HU, Wufem BM (2005)
An ethnobotanical survey of Bauchi State herbal plants and their antimicrobial activity.
Adedapo AA, Abatan MO, Idowu SO, Olorunsogo OO (2005) Effects of chromatographic frac-
tions of Euphorbia hirta on the rat serum biochemistry. Afri J Biomed Res 8(3):185–189
Adejo GO, Eseyin JS, Akintayo CO, Obinna M (2015) Morinda lucida leaf and fruit extracts may
attenuatediarrhea arising from ebola. Basic Res J Med Clin Sci 4(1):95–100
Ahmad RG, Saeidnia S (2011) Photochemistry of Cuminum cyminum seeds and its standards from
field to market. Pharma J 3(25):1–5
Ajayi CO, Elujoba AA, Bejide RA, Akinloye JA, Omonisi AE (2014) Toxicity and pharmacognos-
tic standards for laxative properties of Nigerian Cassia sieberiana and Senna obtusifolia roots.
Eur J Med Plants 6(2):110–123
Anandaraj M, Sastry EVD (2015) Soils, Plant growth and Crop production – Cumin, Fenneland
Fenugreek. http://www.eolss.net/Eolss-sampleAllChapter.aspx. Accessed on 12 Apr 2015
Anuka JA, Yaro AH, Wannang NN, Ezenwanne EB, Yakasai IA (2005) Some in vivo and in vitro
studies of the aqueous leaf extract of Phyllanthus muellerianus (Euphorbiaceae) in laboratory
animals. J Pharm Biores 2(2):93–99
Arbonnier M (2004) Trees, shrubs and lianas of West African dry zones. CIRAD Margraf
Publishers Gmbh, MNHN, Paris, France, p 573
Arya V, Yadav S, Kumar S, Yadav JP (2010) Antimicrobial activity of Cassia occidentalis L (leaf)
against various human pathogenic microbes. Inter J Life Sci Med Res 9:1–11
Asase A, Oteng-Yeboah AA, Odamtten GT, Simmonds MSJ (2005) Ethnobotanical study of some
Ghanaian anti-malarial plants. J Ethnopharmacol 99:273–279
Ashaf AT, Olunu OO (2011) Toxicological evaluation of ethanolic root extract of Morinda lucida
(L.) Benth. (Rubiaceae) in male Wistar rats. J Nat Pharm 2:108–114
Asmah R, Susi E, Abdah MA, Patimah I, Taufiq YH, Mohd FAB (2006) Anticarcinogenic proper-
ties of Strobilanthes crispus extracts and its compounds in vitro. Inter J Cancer Res 2(1):47–49
Asuku O, Atawodi SE, Onyike E (2012) Antioxidant, hepatoprotective, and ameliorative effects of
methanolic extract of leaves of Grewia mollis Juss. on carbon tetrachloride–treated albino rats.
J Med Food 15(1):83–88
Atawodi SE (2005) Antioxidant potentials of African medicinal plants. Afr J Biotechnol
4(2):128–133
Atawodi SE, Preussmann R, Spiegelhalder B (1995) Tobacco-specific nitrosamines in some
Nigerian cigarettes. Cancer Lett 97:1–6
Atawodi SE, Atawodi JC, Idakwo P, Pfundstein B, Haubner R, Wurtele G, Spiegelhalder B, Bartsch
H, Owen RW (2009) Evaluation of the polyphenol composition and antioxidant activity of
african variety of Dacryodes edulis (G.Don) H.J Lam Fruit. J Med Food 12(6):1321–1325.
doi:10.1089/jmf.2008.0215
Atawodi SE, Ogunbusola F (2009) Evaluation of anti-trypanosomal properties of four extracts of

leaves, stem and root barks of prosopis africana in laboratory animal. Biokemistri 21(2):101–108
Atawodi SE, Onaolapo GS (2010) Comparative in vitro antioxidant potential of different parts of
Ipomoea asarifolia, Roemer & Schultes, Guiera senegalensis, J. F. Gmel and Anisopus mannii
N. E. Brown. Braz J Pharm Res 46(2):245–250
Atawodi SE, Adekunle OO, Bala I (2011) Antioxidant, organ protective and ameliorative prop-
erties of methanol extract of Anogeissus leiocarpus Stem Bark against carbon tetrachloride-
induced liver injury. Internl J Pharmaceut Sci Res 2(6):748–753
Atawodi SE,Olowoniyi OD, Adejo GO, Liman ML (2014a) Review of the antioxidant potential
of African medicinal plants and food plants. In: Dubey NK (ed) Plants as a source of natural
antioxidants. CAB International, UK, 63 pp
Atawodi SE, Olowoniyi OD, Obari MA, Ogaba I (2014b) Ethnomedical survey of Adavi and
Ajaokuta Local Government Areas of Ebiraland, Kogi State, Nigeria. Ann Res Rev Biol
4(24):4344–4360
Atawodi SE, Olowoniyi OD, Daikwo MA (2014c) Ethnobotanical survey of plants used for the
management of hypertension in the Igala Speaking Area of Kogi State, Nigeria. Ann Res Rev
Biol 4(24):4535–4543
Bedu-Addo K (1993) Effect of the crude extract of the root of Rauwolfia vomitoria on the repro-
duction of female albino rats (Rattus norvegicus). Kwame Nkrumah University of Science and
Technology, Kumasi, Ghana, Thesis
Bekalo TH, Woodmatas SD, Woldemariam ZA (2009) An ethnobotanical study of medicinal plants
used by local people in the lowlands of Konta Special Woreda, southern nations, nationalities
and peoples regional state. Ethiopian J Ethnobiol Ethnomed 5:26
Bokaeian M, Shiri Y, Bazi S, Saeids S, Sabi Z (2014) Antibacterial activities of Cuminum cyminum
Linn essential oil against multi-drug resistant Escherichia coli. Inter J of Infect 1(1)
Bello OO, Bello TK, Bankole SA (2013) Occurrence of antibioticresistant Staphylococcus aureus
in some street-vended foods in Ogun State, Nigeria. J Adv Biol 1(1):21–28
Boyraz N, Ozcan M (2005) Antifungal effect of some spicehydrosols. Fitoter 76:661–665
Brusto G, Cesari I, Gilardoni G, Tosi S, Grisoli P, Picco AM, Caccialanza G (2012) Chemical
composition and antimicrobial activity of Phyllanthus muellerianus (Kuntze) Excel essential
o. J Ethnopharm 143(3):657–662
Burkill HM (1994) The useful plants of West Tropical Africa, 2nd edn, vol 2, Families E–I. Royal
Botanic Gardens, Kew, Richmond, UK, 636 pp
Burkill HM (2000) The useful plants of West Tropical Africa, 2nd edn, vol 5, Families S–Z,
Addenda. Royal Botanic Gardens, Kew, Richmond, UK, 686 pp
Chaudhary N, Husain SS, Ali M (2014) Chemical Composition and antimicrobial activity of vola-
tile oil of the seeds of Cumimum cyminum. World J Pharm Pharma Ser 3(7):1428–1441
Daniyan SY, Oloruntimilehin JB, Ifeadi O (2011) Antibacterial activity of Cassia occidentalis
flower vegetable extract on selected bacteria. Asian J Biomed Pharm Sci 1(1):23–27
DeMartino L, De Feo V, Fartianni F, Nazzaro F (2009) Chemistry, oxidant, antibacterial and anti-
fungal activities of volatile oils and their components. Nat Prod Commun 4(12):1741–1750
Edwin JE, Dubey S, Gupta S, Gupta VB (2007) Hepatoprotective activity of Euphorbia hirta Linn.
Geo Bios 34(1):81–83
Efiom OO, Oku E (2012) Isolation And Characterization of Triterpene Lup-20-EN-3-Ol And
1,3-Hexyloxacyclotridec-10-EN-2-One From the root of Grewia Mollis. N Y Sci J 5(11)
El-Said AHM, Goder EH (2014) Antifungal activities of Cuminum cyminum and Pimpinella
anisum essential oils. Int J Curr Microbiol Appl Sci 3(3):937–944
Ene AC, Atawodi SE, Ndukwe I, Kwanashie HO (2009) Bioassay-guided fractionation and in vivo
antiplasmodial effect of fractions of chloroform extract of Artemisia maciverae Linn. Acta
Trop 112:288–294. doi:10.1016/j.actatropica. 2009.08.016
Fapojuwomi OA, Asinwa IO (2012) Assessment of medicinal values of Rauvolfia vomitoria
(Afzel) in Ibadan Municipality. Greener J Med Sci 3(2):037–041
Focho DA, Ndam WT, Fonge BA (2009) Medicinal plants of Aguambu Bamumbu in the Lebialem
highlands, southwest province of Cameroon. Afri J Pharm Pharmacol 3(1):001–013
Hanan MA, Musarat A, Azza ME (2012) Biological evaluation of constituents from Grewia mollis.
J Chem Pharm Res 4(1):50 8–518
Hiermann A, Bucar F (1994) Influence of some traditional medicinal plants of Senegal on prosta-
glandin biosynthesis. J Ethnopharmacol 42(2):111–116
Ishak FD, So’ad SZM, Jauhari AHA, Mashid NN, Hassan NM (2013) In vitro study of antimicro-
bial activity of Acalypha indica Linn extract. Open Conf Proceed J 4(2):57–60
Jagatheeswari D, Deepa J, Ali HSJ, Ranganathan P (2013) Acalypha indica L- An important
medicinal plant: a review of its traditional uses, and pharmacological properties. Int J Res Bot
3(1):19–22
Jimoh FO, Oladiji AT (2005) Preliminary studies on Piliostigma thonningii seeds: proximate anal-
ysis, mineral composition and phytochemical screening. Afri J Biotechnol 4(12):1439–1442
Johnson PB, Abdurahman EM, Tiam EA, Abdu AI, Hussaini IM (1999) Euphorbia hirta leaf
extracts increase urine output and electrolytes in rats. J Ethnopharmacol 65(1):63–69
Johri RK (2011) Cuminum cyminum and Carum carvi: an update. Pharmacogn Rev 5(9):63–72
Kerharo-Adam JG (1974) Pharmacopée sénégalaise traditionnelle,plantes médicinales et toxiques.
Vigot et Frères, Paris p 1011
Khosh-Khui M, Bonyanpour AR (2006) Effects of some variables on seed germination and seed-
ling growth of Cumin (Cuminum cyminum L.) Int J Agric Res 1:20–24
Kucinggalak KG (2015) http://www.forestry.gov.my/herba/Kucinggalak_en.pdf. Accessed on the
8 May 2015
Latham P (2004) Useful plants of Bas-Congo province. Democratic Republic of the Congo. DFID,
London, UK. 320 pp. Int J Pharm 34(5):355–358
Lanhers MC, Fleurentin J, Cabalion P, Rolland A, Dorfman P, Misslin R, Pelt JM (1990) Behavioral
effects of Euphorbia hirta sedative and anxiolytic properties. J Ethnopharmacol 29(2):189–198
Lemessa D (2010) Piliostigma thonningii (Scumach) Milne-Redh. Internet: Brink M, Achigan-
Dako EG (Ed). Prota 16: Fibres/Plantas a fibres. PROTA. Wageningen, Netherlands http://
www.prota4u.org. Assessed 25 May 2015
Leporatti ML, Ghedira K (2009) Comparative analysis of medicinal plants used in traditional
medicine in Italy and Tunisia. J Ethnobiol Ethnomed 5:31
Modena ÉDS, Pires ACV, Barônio GJ, Inforzato I, Demczuk SDB (2012) Do fruit traits of the
Senna occidentalis weed influence seed predation by Bruchinae? Braz J Biosci 10(3):293–297
Nartey ET, Ofosuhene M, Kudzi W, Agbale CM (2012) Antioxidant and gastric cytoprotective
prostaglandin properties of Cassia sieberiana root bark extract as an anti-ulcerogenic agent.
BMC Compl Altern Med 12:65
Newton LE (2008) Euphorbia unispina N.E.Br. Internet: Schmelzer GH, Gurib-Fakim A (Ed)
Plant Resources of Tropical Africa / Ressources vegetales de l’Afrique tropicale, Wageningen,
Netherlands. http://www.prota4u.org/search.asp. Assessed 24 May 2015
Nwodo NJ, Okide GB, Okonta JM, Ebebe IM (2003) Antidiabetic effect of Rauwolfia vomitoria
ethanolic leaf extract in rabbits. J Trop Med Plants 4(1):71–74
Odeja OO, Obi G, Ogwuche CE, Elemike EE, Oderinlo OO (2014) Phytochemical screening,
antioxidant and antimicrobial activities of Senna occidentalis (L) leaves. Int J Herb Med
2(4):26–30
Ogbe AO, Ditse U, Echeonwu I, Ajodoh K, Atawodi SE, Abdu PA (2009) Potential of a wild
medicinal mushroom, Ganoderma sp., as feed supplement in chicken diet: effect on perfor-
mance and health of pullets. Int J Poult Sci 8(11):1052–1057
Ogbulie JN, Ogueke CC, Okoli IC, Anyanwu BN (2007) Antibacterial activities and toxicological
potentials of crude ethanolic extracts of Euphorbia hirta. Afr J Biotechnol 6(13):1544–1548
Olapade AA, Ajayi OA, Ajayi IA (2014) Physical and chemical properties of Cassia sieberiana
seeds. Int Food Res J 21(2):67–772
Onwuliri F, Ndako J, Olabode AO, Echeonwu GON, Onwuliri EA, Nwankiti PP, Ohaeri MC,
Amona GE (2009) Phytochemical screening and antimicrobial activity of Guiera senegalensis
leaves. Int J Trop Agric Food Sys 3(2)
Oshobu MA, Geidam MA (2014) Effects of aqueous ethanolic extract of the leaves of Guiera
senegalensis J.F.Gmel (Combretaceae) on liver function in Wister strain albino rats. Int J Sci
Res Manag 2(3):611–615
Oyewale AO, Mika A, Peters FA (2002) Phytochemical, cytotoxicity and microbial screening of
Euphorbia hirta Linn. Glob J Pure Appl Sci 8(1):49–55
Ozolua RI, Alonge P, Igbe I (2009) Effects of leaf extracts of Piliostigma thonningii Schum on
aortic ring contractility and bleeding time in rats. J Herb Spices Med Plants 15:326–333
Parashar M, Jakhar ML, Mabik CP (2014) A review on biotechnology, genetic diversity in cumin
(Cuminum cyminum). Int J Life Sci Pharmacogn Rev 5(9):63–73
Rajaselvam J, Benila SJM, Meena R (2012) A study of antimicrobial activity of Acalypha indica
against selected microbial species. Int J Pharma Sci Res (IJPSR) 3(9):473–476
Rao CV, Rao GMM, Kartik R, Sudhakar M, Mehrotra S, Goel RK (2003) Ulcer protective effect
of Euphorbia hirta extract in rats. J Trop Med Plants 4(2):199–205
Sadiq IS, Shuaibu M, Bello AB, Tureta SG, Isah A, Izuagie T, Nasiru S, Kamaru MB (2012)
Phytochemistry and antimicrobial Activities of Cassia Occidentalis used for herbal remedies.
J Chem Eng 1(1):38–41
Saidu AN, Aina EO, Mann A, Leje UI (2011) The effect of aqueous extract of leaves on rat infected
with Salmonella typhi. Aus J Basic Appl Sci 5(12):1863
Salihu SO, Usman AA (2015) Antimicrobial and phytochemical study of the bioactive fractions
of Guiera senegalensis from Alasan Tambuwal, Nigeria. J Pharm Phytochem 3(6):106–111
Sanogo R (2012) Guiera senegalensis J.F. Gmel. Internet: Schmelzer GH, Gurib-Fakim A (ed).
Prota 11(2): Medicinal plants/plantes medicinales 2. PROTA, Wageningen, Netherlands. http://
www.prota4u.org. Accessed 4 May 2015
Schmelzer GH, Gurib-Fakim A (2008) Plant resources of tropical Africa medicinal plants 1 PROTA
Foundation, Wageningen, Netherlands/Backhiys Publishers, Leiden. Netherlands CTA 11(1)
Silva O, Serrano R, Gomes ET (2008) Botanical characterization of Guiera senegalensis leaves.
Micro Microanal 14(5):398–404
Singh P, Khandelwal P (2010) Cetyltriacontanoate and other constituents from Acacia jacquemon-
tii and Kigelia pinnata. J Indian Chem Soc 87:1403–1407
Soladoye MO, Amusa NA, Raji-Esan SO, Chukwuma EC, Taiwo AA (2010) Ethnobotanical sur-
vey of anticancer plants in Ogun State, Nigeria. Annals Biol Res 1(4):261–273
Sombie PAED, Hilou A, Mounier C, Coulibaly AY, Kiendrebeogo M, Millogo JF, Nacoulma OG
(2011) Res. J Med Plants 5(4):448–461
Somboro AA, PatelK DD, Sidibe L, Chalchat JC, Figuredo G, Ducki S, Troin Y, Chalard P (2011)
An ethnobotanical and phytochemical study of the African medicinal plant Guiera senegalen-
sis J.F.Gmel. J Med Plants 5(9):1639–1651
Somchit MN, Mutalib AR, Hasmawie MR, Murni A (2001) In vitro antifungal and antibacterial
properties of Euphorbia hirta. J Trop Med Plants 2(2):179–182
Terashima H, Ichikawa M (2003) A comparative ethnobotany of the Mbuti and Efe hunter-
gatherers in the Ituri forest, Democratic Republic of Congo. Afr Study Monogr 24(1–2):1–168
Tona L, Cimanga RK, Mesia K, Musuamba CT, De Bruyne T, Apers S, Hernans N, Van Miert
S, Pieters L, Totté J, Vlietink AJ (2004) In vitro antiplasmodial activity of extracts and frac-
tions from seven medicinal plants used in the Democratic Republic of Congo. J Ethnopharm
93:27–32
Ullah MF, Khan MW (2008) Food as medicine: potential therapeutic tendencies of plant derived
polyphenolic compounds. Asian Pac J Cancer Prev 9:187–196
Van der Maesen LJG (2007). Cassia sieberiana DC. Internet: Schmelzer GH, Gurib-Fakim A
(ed) Prota 11(1): Medicinal plants/plantes medicinales 1. [CD-Rom] PROTA, Wageningen,
Netherlands. http://www.prota4u.org/search.asp. Assessed 24 May 2015
Van-Dilst FJH, Leeuwenberg AJM (1991) Rauvolfia L. in Africa and Madagascar. Series of revi-
sions of Apocynaceae 33. Bulletin du Jardin Botanique Nationale
Vashishtha VM, John T, Kumar A (2009) Clinical and pathological features of acute toxicity due
to Cassia occidentalis in vertebrates. Indian J Med Res 130:23–30
Vijayalakshmi S, Ranjitha J, Devi Rajeswari V, Bhagiyalakshmi M (2013) Pharmacological profile
of Cassia occidentalis – a review. Int J Pharm Pharma Sci 5(3):29–33
Wallace AP, Marko KE, Wisdom AP, Dennis E (1990) Comparative clinical studies of three plant
leaves as sources of protein using albino rats. J Ghana Sci Assoc 2(3):81–87
Phytochemical, Pharmacological
and Therapeutic Potentials of Some Wild
Nigerian Medicinal Trees
Sunday E. Atawodi, Olufunsho D. Olowoniyi, Godwin O. Adejo,

Abstract With its location in an extensive geographical area that spread from the
Mangrove forest in the south to the Sahel savannah in the north, Nigeria has diverse
tropical vegetation with varying economic and medicinal significance. The vegeta-
tion, among other uses is a source of herbal medicine on which a large proportion of
its populace relies for primary health care. Trees have a large repository of phyto-
chemicals, to which the various pharmacological and therapeutic attributes can be
ascribed. This article is a concise review of nine trees with medicinal importance
belonging to eight families, which are widely used in Nigerian traditional and indig-
enous medicine. For each plant, information on the taxonomy, morphology, geo-
graphical distribution, ecological requirements, major chemical constituents and
bioactive compounds, traditional uses and medicinal uses based on biological activ-
ities, as well as uses supported by clinical data are provided.
Keywords Trees • Medicinal plants • Phytochemicals • Pharmacology • Therapeutic

potential • Herbal medicine • Nigeria
1 Introduction
Nigeria, is the most populous country in Africa, having a landmass of over

923.768 km2 enclosed within latitudes 4°161 N and 13°521 N of the equator and
between longitudes 2°491 E and 14°371 E of Greenwich Meridian. Within the varied
ecological zones to which the country belongs is an array of floral species. There are
7895 plant species from 338 families and 2215 genera that have been identified in
Nigeria. Its moist forests are also rich in epiphytic ferns and orchids and contain
Dedicated to all those that have made life worth living

S.E. Atawodi (*) O.D. Olowoniyi • G.O. Adejo • M.L. Liman

DOI 10.1007/978-94-024-1120-1_11
over 560 species of trees which attain heights of at least 12 m and girth of 60 cm
(Borokini et al. 2010).
Apart from making the biomolecules that are universally required by living
organisms, plants also synthesize a large number of complex chemicals that are
unique to them. Phytochemicals are products of secondary metabolism defined vari-
ously as a metabolic cul de sac or peripheral metabolic pathways leading off from
the universal (or primary) metabolism. Phytochemicals range from cell wall sub-
stances, through photosynthetic pigments, terpenes and terpenoids, the alkaloids,
plant phenolics to plant hormones, non-protein amino acids and cyanogenic glyco-
sides. Many phytochemicals exhibit bioactive activity in other living organisms and
are useful as therapeutic agents, pesticides, food additives and other biologicals
(Onwuliri et al. 2006).
Medicinal plants are plants whose one or more of their organs contain active
ingredients which can be used for therapeutic purposes or contain compounds that
can be used for the synthesis of useful drugs. The medicinal values of these plants
lie in the bioactive phyto-constituents that produce definite physiological action on
animals (Afolabi and Afolabi 2013). Herbal medicines have been widely utilized as
effective remedies for the prevention and treatment of multiple health conditions by
almost every known culture. Thus, for over 5000 years, the Chinese and Indians
have used herbal medicine for their health care (Rivera et al. 2013), steming from
the fact that herbal medicine is cheaper, easily accessible, easy to prepare and devoid
of severe side effects (Fasola et al. 2010). In Nigeria, plants are used for the manage-
ment of oxidation-associated diseases (Atawodi 2005; Atawodi et al. 2014a) and
other ailments (Atawodi et al. 2014b; Atawodi et al. 2014c). To add to that body of
knowledge in the most populous black nation, this article is a further compilation of
notable Nigerian medicinal trees.
2 Annonaceae
Annona muricata Linn (Synonyms: Annona macrocarpa, A. bonplandiana,

A. cearensis, Guanabanus muricatus)
(Source: biogeodb.stri.si.edu)
Phytochemical, Pharmacological and Therapeutic Potentials of Some Wild Nigerian… 285
Taxonomy
Annona muricata Linn commonly known as graviola, soursop, paw-paw,
guanabana, adunu, sorasaka, Mullaatha, Thorny custard apple, Shul-ram-fal, or
Hanuman fal. belongs to the family of Annonaceae (Sulaiman et al. 2012; Mishra
et al. 2013).
It is a flowering, evergreen tree with dark green leaves, which are lanceolate, glossy
and broad, (Sulaiman et al. 2012; Mishra et al. 2013). Growing up 5–6 m in height,
the young branchlets are rusty-hairy, the malodorous leaves, normally evergreen,
are alternate, smooth, glossy, dark green on the upper surface, lighter beneath;
oblong, elliptic or narrow-obovate, pointed at both ends, 6–20 cm long and 2–6 cm
wide. The flowers are borne singly, may emerge anywhere on the trunk, branches or
twigs. They are short stalked, 4–5 cm long, plump, and triangular-conical; the 3
fleshy, slightly spreading, outer petals yellow-green, with three close-set inner pet-
als, pale-yellow. The fruit is more or less oval or heart-shaped, sometimes irregular,
lopsided or curved, due to improper carpel development or insect injury. The size
ranges from 10–30 cm long and up to 15 cm in width and the weight may be up to
4.5–6.8 kg. The fruit is compound and covered with reticulate, leathery-appearing
but tender, inedible bitter skin from which protrude few or many stubby, or more
elongated and curved soft, pliable “spines” (Adewole and Caxton-Martins 2006).
It is a typical tropical tree with heart-shaped edible fruits and is widely distributed
in most of tropical countries, (Adewole and Caxton-Martins 2006), including sub-
Saharan African countries like Nigeria that lie within the tropics and sub-tropics
(Adewole and Caxton-Martins 2006; Mishra et al. 2013; Sulaiman et al. 2012).
However, it is native to Mexico, Cuba, Central America, the Caribbean and northern
South American countries, including Colombia, Brazil, Peru and Venezuela (Mishra
et al. 2013).
It is adapted to areas of high humidity and relatively warm winters; usually, tem-
peratures below 5 °C (41 °F) will cause damage to leaves and small branches, while
temperatures below 3 °C (37 °F) can be fatal, as the fruit becomes dry and unfit for
consumption (Mishra et al. 2013).
Among the chemical constituents found in the leaf of Annona muricata are
alkaloids, essential oils and annonaceous acetogenins (Atawodi 2011, Sulaiman
et al. 2012).
Traditional Use (Part(s) Used) and Common Knowledge
Traditionally, the leaf has been used in the treatment of headaches, hypertension,
cough, asthma and as an antispasmodic, sedative and nervine for heart condition
(Sulaiman et al. 2012). It has also been reported to exhibit anti-inflammatory and
analgesic effects (Sulaiman et al. 2012).
The fruit has been traditionally applied for cough, hypertension, rheumatism,
tumors, cancer, asthma, childbirth, lactagogue, tranquilizer and in liver disorders, as
well as arthritis, where extracts are applied externally. The leaf is also used for treat-
ing headaches, insomnia, cystisitis, liver problems, diabetes and as anti-inflammatory,
anti-spasmodic and anti-dysenteric agents. The decoction of the leaf has parasitic,
anti-rheumatic and neuralgic effects when used internally, while when cooked, the
extract of leaf is applied topically against rheumatism and abscesses (Mishra
et al. 2013).
Modern Medicine Based on Traditional Medicine Uses (Biological Activities)
The leaf and seed of the tree have long been used by native peoples for treating and
managing an astounding variety of ailments, ranging from parasites (seed), to high
blood pressure and cancer (Adewole and Caxton-Martins 2006; Mishra et al. 2013).
Uses Supported by Clinical Data, Pharmacopoeias, Well Established
Documents and in Traditional Medicine
Annonaceous acetogenins from Annona muricata L have been found to possess
anti-tumor and anticancer agents in numerous in vitro studies. The compound was
also shown to be selectively toxic against various types of the cancerous cells with-
out harming healthy cells (Sulaiman et al. 2012). Ethanolic leaf extract applied topi-
cally on mice skin papillomagenesis at graduated doses of 30, 100 and 300 mg/kg
significantly reduced induced papillomagenesis at all doses in both pre-initiation
and promotion protocols in a dose-dependent manner, and comparable to carcinogen–
treated control (Sulaiman et al. 2012).
Moreover, at 100 and 300 mg/kg, the leaf extract completely inhibited the tumor
development in all stages, thus suggesting the suppression of tumor initiation as
well as tumor promotion even at lower dosage by A. muricata leaves extract
(Sulaiman et al. 2012). Research has further validated the use of various parts of
A. muricata for hypertensive, antispasmodic, vasodilatory and cardio depressive-
conditions (Mishra et al. 2013).
3 Asclepiadaceae
Parquetina nigrescens (Afzel.) Bulock
Synonyms Periploca nigrescens

Common and Local Names
Ewe Ogbo (Yoruba) (Odetola et al. 2006)
Parquetina nigrescens has a woody base, up to 8 m long, glabrous, latex copious.
The leaves are simple and entire, almost sessile, blade oblong, (7.5-)12.5–14(−16)
cm × (3-)4.5–7.5(−11) cm, apex rounded, suddenly long-acuminate, base rounded
to almost cordate, green and often glossy above, pale green beneath, (thin) leathery,
with conspicuous lateral veins. Inflorescence an open axillary cyme, 15–30 flow-
ered. Flowers bisexual, 5-merous, regular, pedicel 6–10 mm long; sepals very
broadly ovate, about 2.5 mm long, apex obtuse; corolla with very short tube, lobes
fleshy, spreading, elliptical to ovate, 10–12 mm × 4–5 mm, reflexed, apex rounded,
above velvety, dark brown at apex; corona lobes bifid, filiform, 5–7 mm long, cross-
shaped because of 2 lateral segments above middle, apical segments curled, green-
ish white to pale yellow; stamens with hairy filaments, pollen carriers spoon-shaped;
ovaries 2, free, semi-inferior, almost globular, gynostegium excerted, styles fused,
apex 5-angled. The fruit is a pair of follicles, horizontal, each follicle narrowly
ovoid, 12–21 × 12–20 mm, 2-ridged, apex slightly curved, many-seeded. Seeds
narrowly elliptical to elliptical, brown to dark brown, warty, tuft of hairs white
(Alvarez Cruz 2012).
(Source: Authors)
Parquetina nigrescens Afzel. occurs in a large part of Africa, from Senegal east to
Sudan and south through Central and East Africa to Zambia, Angola and Eastern
Zimbabwe (Alvarez Cruz 2012).
Parquetina nigrescens grows in secondary forests, savanna, vegetation bordering
roads and gallery forest, also commonly growing on ant-hills. It grows on various
types of soil, including marshy areas (Alvarez Cruz 2012).
Parquetina nigrescens leaf contain cardenolides, glycosides and alkaloids (Odetola
et al. 2006) while saponins, alkaloids, tannins, anthraquinones, terpenoids, flavonoids,
cardiac glycosides and ascorbic acid are reported by Akinyemi and Dada (2013).
The rich cardiac glycosides (cardenolides) collectively called strophanthins are
most abundant in the latex and are responsible for the activity in arrow poison.
Traditional and Common Uses
Different parts of Parquetina nigrescens (leaf, bark, latex and roots) are used for
various disorders of the gastrointestinal tract disorders and as constituents of medi-
cations for treating rickets, diarrhea, skin lesion, menstrual disorders and gonorrhea.
A decoction of the leaf is drunk as aphrodisiac (Odetola et al. 2006). The use of
Parquetina nigrescens as herbal remedy for the management of sickle cell anemia
has also been reported (Imaga 2013). A decoction of the leaf is taken as enema to
treat kidney problems, constipation and to induce abortion. A decoction of the leaf
or whole plant, sometimes with parts of other plant species is taken to treat measles,
intestinal worms, diarrhea, dysentery, diabetes, menstrual disorders and venereal
diseases. The latex and leaf sap cause a burning sensation on the skin and are exter-
nally applied to tumors, abscesses, sores and burns. They also blacken scars and are
applied as a dressing for wounds. The latex is very toxic and widely used as an
ingredient of arrow poison (Alvarez Cruz 2012).
In a study to evaluate the antimicrobial activity of the aqueous leaf extract of
Parquetina nigrescens, it showed antimicrobial effect against Staphylococcus
aureus, Salmonella typhi, Proteus mirabilis, Pseudomonas aeruginosa, Bacillus
subtilis and Preteus vulgaris while the ethanolic extract only had effect on
Pseudomonas aeruginosa and Salmonella typhi (Odetola et al. 2006).
4 Bignoniaceae
Kigelia africana (Lam.) Benth
Synonym Kigelia pinnata, Kigelia aethiopica

Common and Local Names Sausage tree (English); Rawuya (Hausa); Uturubein
(Igbo); Pandoro, Iyan (Yoruba); Mwegea (Swahili); Umfongothi (Zulu) (Grace and
Davis 2002).
(Source: Authors)
The tree is a semi-deciduous to deciduous tree that grows up to 25 m tall. The bark
is grey and smooth and flakes in older specimens. Leaves are crowded near the tips
of branches, and young leaves are brownish-red. Flowers bloom in long, loose, pen-
dulous spray of 5–12 flowers. Petals are a deep, velvety red with yellow veining on
the outside. The cylindrical fruit is pendulous on a long fruit stalk. The fruit can
grow up to 1.0 m long and 20 cm wide, and is grey and rounded at the apex. The tree
flowers from August to October, and fruits from December to June.
Depending on the climate, the sausage tree is remarkably fast-growing and can
mature in 4–5 years. Ripe fruits can weigh up to 12 kg and can cause considerable
damage when they drop. With its fast growth rate, spreading canopy, and interesting
flowers and fruit, Kigelia africana is a popular street tree. It can also be used suc-
cessfully for bonsai, because the thick stem makes for an attractive feature (Watt
and Brayer-Brandwijk 1962). The fruit is indehiscent, with woody wall and heavily
marked with lenticels at the surface. It is grey-brown and many seeded when
matured (Olatunji and Atolani 2009).
Kigelia africana (Lam) occurs throughout tropical Africa (Saini et al. 2009) but
does not occur in Mauritania, Sao Tome and Principe, or the Indian Ocean Islands.
It has been introduced as an ornamental to Cape Verde and Madagascar, Pakistan,
India, China, South East Asia, Australia, Hawaii and Central and South America
(Grace and Davis 2002).
Kigelia africana grows along watercourses, in riverine fringes, alluvial and open
woodland, high rainfall savanna, shrub-land and in rain forest. It occurs on loamy
red clay soils, sometimes, rocky, damp or peaty, from sea level up to zoom altitude
(Olatunji and Atolani 2009).
The root and bark of Kigelia africana have naphthoquinone, lapachol and the dihy-
droisocoumarin kigelin as major compounds. Several other compounds including
the naphthaquinoids kigelinone, pinnatal and isopinnatal, and the sterols stigmas-
terol and β-sitosterol have been isolated from the bark. The flavonoids
6-hydroxyluteolin-7-α-glucoside and luteolin have been isolated from the fruits and
the leaves, while the root has also yielded dihydroisocoumarins, lapachol and ste-
rols, and the presence of iridoid glycosides also have been reported (Govindachari
et al. 1971; Alamelu and Bhuwan 1974).
A study of the heartwood identified the presence of lapachol, dehydro-α-
lapachone, tecomaquinone-I, D-sesamin, paulowin, kigeliol, kigelinone, β-sitosterol
and stigmasterol (Singh and Khandelwal 2010). The major iridoids found in the root
bark and stem bark of Kigelia africana are specioside, verminoside and minecoside
(Houghton and Akunyili 1993). The iridoids possess anti-inflammatory properties
(Gouda et al. 2003; Kupeli et al. 2005). Also, those same iridoids were isolated from
the stem bark and they possess anti-amoebaic properties (Bharti et al. 2006). Further
analysis of the fruits identified four new iridoids, namely, 7 – hydroxyviteoid II,
7-hydroxyeucommic acid, 7-hydroxyl-10-deoxyeucommiol and 10 – deoxyeucom-
miol. Seven known compounds, namely jiofuran, jioglutolide, 1-dehydroxy-3,4-
dihydroaucubigenin, des-p-hydroxybenzoyl kisasagenol B, ajugol, verminoside and
6-transcaffeoyl ajugol were also identified (Gouda et al. 2003). A study of the anti-
microbial properties of the aqueous stem back extract of Kigelia africana revealed
the presence of two naphthaquinones, kigelinone and isopinnatal (Akunyili et al.
1991; Akunyili and Houghton 1993).
The hexane extract of the leaf of Kigelia africana has been reported to be rich in
hydrocarbons and some volatile compounds. They include n–hentriacontane,
1-
tricosene, 11-(2,2-dimethyl) propylheneicosane, 2,6,10–trimethyldodecane,
penta fluoroheptadecyl ester, 2–ethylhexyloctadecyl sulfurous acid ester, heneico-
sane and hexyloctyl–sulfurous acid ester. Others are 4,4-dimethyl undecane,
methyl-12-methyltetradecanoate, 1-iodohexadecane and 1- iododecane.
Hentriacontane have been reported to have a possible anti–tumour activity while
methyl-12-methyltetradecanoate has also been reported for its inhibition capacity
on the development of coneal angiogenesis, which is responsible for blindness and
other infections (Atolani and Olatunji 2010).
Traditional and Common Uses
The powdered fruit of Kigelia africana is applied as a dressing in the treatment of
wounds, abscesses and ulcers. The green fruit is used as a poultice for syphilis and
rheumatism, and a poultice made from the leaves is used as treatment for backache.
An infusion is made from the ground bark and fruits to treat stomach problems in
children, and an infusion from the root and bark is taken to treat pneumonia. A
decoction made from the bark is gargled to relieve toothache (Jackson and Beckett
2012). Kigelia africana is also widely used for a variety of skin complaints. It is
used in both traditional and orthodox medicines to treat malignant neoplasms such
as skin melanoma, tumors and breast cancer. Traditional preparations include
extracts, poultices and powders of the bark or fruit, topical creams containing
extracts of the fruits are produced commercially (Grace and Davis 2002). Snakebite
antidotes are made with an infusion of the parts, taken orally or rubbed onto the bite.
A fruit decoction is also used to treat edema of legs (Grace and Davis 2002).
Infectious diseases including leprosy and impetigo, dermal complaints and infec-
tions such as whitlows, eczema, cysts, acne, boils and psoriasis are treated with
traditional medicines containing the fruits and less frequently the bark (Grace and
Davis 2002).
The powdered fruit is applied as a dressing for ulcers, while unripe fruit is used
as a dressing for wounds, hemorrhoids and rheumatism. Venereal diseases are com-
monly treated with the bark extracts, usually in palm wine as oral medication. The
fruits and bark, ground and boiled in water are also taken orally or used as an enema
in treating children’s stomach ailments arising from infestation by worms (Grace
and Davis 2002; Saini et al. 2009). Aqueous preparations of the root, fruit and flow-
ers are administered orally or as a vaginal pessary to treat infections. The fruits and
bark are used to promote breast development in young women, or in contrast, to
reduce swelling and mastitis of the breast. The fruits are further employed as a
galactogue and a decoction of the bark and leaves is administered as an abortifa-
cient. Sexual complaints such as infertility, poor libido, sexual asthenia and impo-
tence are treated with medicines containing the fruits, roots or leaves.
A small quantity of unripe fruit is chewed, while an aqueous preparation or prep-
arations in local beer including palm wine is taken orally as a sexual stimulant and
aphrodisiac (Grace and Davis 2002). Fungal infestation such as ringworm, mycosia
and athletes’ foot are washed with the water in which the bark has been macerated,
and preparation containing the leaves and fruits applied locally (Grace and Davis
2002). Aqueous extract of the leaves is applied to treat diarrhea, while that of the
stem bark is for epilepsy. Parts of the plant are also used to treat malaria, diabetes
and postpartum hemorrhage. For ethno-veterinary medical application, the fruits
and sometimes other parts of Kigelia africana are used to treat digestive system
disorders, leg edemas, dermal irritations and infections, mastitis and retained pla-
centa. Brucellosis and Newcastle disease are also treated with Kigelia africana
(Grace and Davis 2002).
Among the many uses of Kigelia africana in traditional medicine is in the treatment
of bacterial and fungal infections. In a study to verify these properties, crude aque-
ous, ethanolic and ethyl acetate extracts of stem, bark and fruit were screened for
antibacterial activity. The stem bark and fruit extracts showed similar antibacterial
effects against Gram-negative and Gram-positive bacteria (Grace and Davis 2002).
Owolabi et al. (2007) also reported the antibacterial and antifungal effects of the
crude ethanolic extract of the stem bark, using agar diffusion technique against
Staphylococcus aureus and Candida albicans. The effect of the extract was compa-
rable to that of the standard amoxillin antibiotic.
In another study, the stem bark extract inhibited the growth of Escherichia coli,
Pseudomonas aeruginosa, Staphylococcus aureus and Candida albicans (Akunyili
et al. 1991). Evaluation of the antibacterial activities of ethanolic and aqueous
extracts of Kigelia africana fruit against multi-drug resistant Pseudomonas aerugi-
nosa showed that the ethanolic extract was more potent than the aqueous extract
(Tyagi et al. 2011). Antiprotozoal, anti-inflammatory, analgesic, antidiarrheal, anti-
diabetic, antioxidant and anti-ulcer activities have been reported and scientifically
evaluated for various parts of Kigelia africana (Atawodi and Olowoniyi 2015).
5 Chrysobalanceae
Parinari curatellifolia Planch. Ex Benth
(Source: www.africamuseum.be, http://copperflora.org)

Parinari curatellifolia Planch. Ex Benth (Family: Chrysobalanaceae) is commonly
called cork tree or hissing tree (Maharaj and Glen 2008). It is also called mbola
plum, mobola plum, fever tree (Orwa et al. 2009).
Common Names
Hissing tree, Mbola plum, Mobola plum, fever tree (English), Pobeguin,mendoca
(French), Mbura (Swahili), Grysappel (Afrikaans), (Orwa et al. 2009).
Parinari curatellifolia is an evergreen, rough dark grey barked, spreading tree that
can grow up to 20 m, and that is striking amongst surrounding vegetation because
of its semi-circular, almost mushroom-shaped canopy depicting hues of blue-green
and grey. The young shoots are densely covered with yellow wooly hairs. The leaves
are alternate, single, elliptic to oblong, 3–8 × 2–4 cm, leathery dark green on top.
The leaves are finely velvety when young, but losing the hair later, densely hairy
and grey to yellow underside. The apex is broadly tapering, often notched; base
square, margin entire and has a short petiole. Parinari curatellifolia has small white
and sweet scented flowers that exist in short branched heads or panicles, 4–6 cm in
diameter in leaf axils.
The stalks and calyces are densely covered with yellowish wooly hairs, while the
flowers are bisexual, have five sepals, five petals and seven or more stamens, joined
at the base in a short ring inserted in the mouth of the receptacle. The flower has
two-chambered ovary. The fruit is oval to round, up to 5 × 3.5 cm, russet-yellow to
greyish, scaly and pitted, turns orange-yellow when ripe (Orwa et al. 2009).
Parinari curatellifolia grows across Nigeria and other sub-Saharan Africa countries
downwards up to South Africa, but it is exotic to Madagascar and Seychelles
(Orwa et al. 2009).
Ecological Requirement
Parinari curatellifolia grows naturally in open deciduous woodland. It occurs in
varying climatic regimes and is particularly common near rivers and in areas of poor
drainage. It is sensitive to frost and cold wind. It grows in regions between 0–1900 m
altitude, mean annual rainfall 0–2700 mm and mean annual temperature of 10–30
°C. A wide range of soil types is required for its growth- light-yellowish brown to
reddish yellow, gritty, sandy clay loam and red to dark red friable clays with lateritic
horizon (Orwa et al. 2009).
Phytochemical screening of the stem bark extracts of Parinari curatellifolia revealed
the presence of saponins, balsams, carbohydrates, alkaloids, tannins, cardiac glycosides,
flavonoids, digitalis glycosides, phenol, terpenes and steroid (Peni et al. 2010).
Parinari curatellifolia is used as a remedy for dysentery, epilepsy, malaria,
toothache and venereal diseases (Feitosa et al. 2012) and epiglottis (Wurochekke
et al. 2013).
The aqueous extract of stem bark of Parinari curatellifolia, by hole in plate bioas-
say procedure had inhibitory activity against Staphylococcus aureus and Klebsiella
spp. The methanolic extract was effective against Bacillus subtilis and Pseudomonas
aeruginosa (Peni et al. 2010), while the root bark is also reported to have strong
antioxidant activity (Atawodi et al. 2013).
Parinari curatellifolia is a valuable and cherished medicinal plant in which different
parts of the plant are widely used by the traditional herbalist in the treatment of
diabetes (Atawodi et al. 2013), and other conditions, like hypertension, liver-related
diseases and epiglottitis. The medicinal uses of the fruit extract of Parinari
curatellifolia has also been reported to include its use as cardiac tonic, dyspores and
as diuretic. The leaves are used as expectorant, sedative, anti-inflammatory and in

treating anemia. Traditionally the bark is used for washing clothes, for vaginal douches,
itchy scalp, dandruff, cough and also for treating infections (Peni et al. 2010).
6 Combretaceae
Anogeissus leiocarpus (DC) Guill. & Perr.
Synonyms Anogeissus schimperi Hochest. Ex Hutch & Dalz., Concarpus leiocar-

pus DC.
(Source: P. Poilecot)
Taxonomy
Anogeissus leiocarpus Guill. & Perr. (Combretaceae), Common name: Axle-wood
tree (Victor et al. 2013) is an African indigenous tree commonly found in forest
savannahs of Nigeria and other West African countries (Attiouaaf et al. 2011).
Anogeissus leiocarpus is a woody deciduous tree that can grow up to a height
of15–30 m tall and up to 1 m in diameter (Burkill 1985; Attiouaaf et al. 2011; Arbab
2014). The heights seem to vary as one moves from country to country from Senegal
(shorter) to Nigeria (taller), but with generally sparse crown (Burkill 1985; Attiouaaf
et al. 2011). Heart-wood is dark, dull brown, streaky, becoming sometimes almost
ebony-black, and is very hard, dense and fine-textured, while the bark is generally
grey and scaly (Burkill 1985). Branches are drooping and slender, with leaves
arranged in alternate, ovate–lanceolate in shape, 2–8 cm long and 1.3–5 cm across
(Arbab 2014). The leaves are acute at the apex and attenuate at the base, pubescent
beneath. Inflorescence globose heads, 2 cm across, yellow; the flowers are bisexual,
petals absent. Fruits are globose cone like heads (Arbab 2014).
The fruits of A. leiocarpus contain about 40 wind-dispersed seeds of 10 mg each.

Each fruit is broadly winged, dark grey, 3 cm across. It can reproduce by seeds as
well as vegetative propagation. The seeds ripen during the dry season and germinate
mainly at the on-set of the rainy season (Hennenberg et al. 2005).
In Africa, its occurrence extends from Senegal in West Africa to Sudan and Ethiopia
in East Africa. Those growing in the driest area tend to have smaller leaves and
hairier flowers than those growing under wetter conditions, but both differences
are not sufficiently marked to create distinct varieties (Hennenberg et al. 2005;
Atawodi 2011).
Anogeissus leiocarpus thrives around the Guinea sub-humid dry forest stretching
across a vast area that forms the ‘Guineo-Congolian’ rainforest, where rainfall
ranges from 1200–1500 mm/year in the east to 1500–2000 mm/year in the west
(Chidumayo and Gumbo 2010). Soils in the dry forest and woodland areas are rela-
tively shallow with less than 1.5 m depth and high content of sand ranging from
30–90%, clay content of between 5–20% and silt making up between 5–45%. Silt-
loamy soil is found in the eastern part of Africa to sandy-loam in the western part,
falling within the areas where the Anogeissus leiocarpus can be found. The most
favorable range of soil pH for the growth of the plant is 5.5–7.5 (Chidumayo and
Gumbo 2010).
Quantitative estimation of bioactive phytoconstituents showed that A. leiocarpus
plant contains alkaloids, phenolics, flavonoids, alkaloids and flavonoids (Barku
et al. 2013). It has been reported that there are 8 flavonoid types present in
A. leiocarpus, namely, cathecin, 4H-1-Benzopyran-4-one, 7-[(6-deoxy-α-L-
mannopyranosyl) oxy]-5-hydroxy-2-(4-hydroxy-3-methoxyphenyl), quercetin, iso-
quercetin, rutin, vitexin, kaempferol, and procyanidin B2 (Attiouaaf et al. 2011;
Barku et al. 2013).
Traditional Uses/(Part(s) Used) and Common Knowledge
The leaf of A. leiocarpus is used externally as a decoction in the eastern part of
Nigeria to treat skin diseases and psoriasis. Powders of the bark are applied on
wounds, sores, boils, cysts and diabetic ulcers and the conditions are alleviated
(Barku et al. 2013). Also in the Volta Region of Ghana, it was reported that the fresh
leaves of A. leiocarpus have been used to heal wounds (Barku et al. 2013). The
decoction and maceration of the stem bark are used against anorexia, constipation,
jaundice, itching, wounds, carbuncles, boils and various forms of ulcers (Harouna
et al. 2012).
Modern Medicine Based on Traditional Medicinal Uses (Biological Activities)
Anogeissus leiocarpus may serve as remedies for gonorrhea, general body pain,
blood clotting agent, diabetes (Atawodi 2011), hypertension and other diseases with
oxidative stress as major etiological factor (Atawodi et al. 2011).
Uses Supported by Clinical Data, Pharmacopoeias, Well Established

Documents and in Traditional Medicine
It was reported that methanolic extract of the stem bark of Anogeissus leiocarpus
possess antioxidant, hepatoprotective and ameliorative effect on hepatocellular
injury via mechanisms believed to be closely related to its variously reported thera-
peutic properties (Atawodi et al. 2011).
Terminalia catappa Linn
Synonyms Terminalia procera
(Source: Authors)

Terminalia catappa Linn (Family Combretaceae) is commonly called tropical
almond.
Common Names Tropical almond, Umbrella tree.
Terminalia catappa is a tree that can attain 25 m tall. The leaves are simple, alter-
nate and clustered towards the end of branches (Jayaweera 1982). They are short
petioled, 15–28 cm long, 10.5–16.5 cm broad, glabrous and shining above and
tormentose below with two glandular depressions near the base of the midrib on
the underside. The leaves turn pink-red–yellow before falling. The flowers are
regular polygamous in simple solitary, axillary, rusty-tormentose or pilose spikes,
6–18 cm long, upper flowers male and lower flowers bisexual, bracts minute, calyx
lobes of five triangular valves, soon deciduous, petals absent, stamens ten inserted
on the calyx lobes, biseriate, 5 lower stamens opposite the calyx teeth and the five
higher ones alternating with them, ovary inferior, unilocular with 2 or 3 pendulous
ovules, glabrous or hairy, ellipsoid, slightly compressed showing two edges. The
fruit is an indehiscent drupe with a hard endocarp. The colour of the fruit is green,
yellow or reddish (Jayaweera 1982).
Terminalia catappa is native to countries of the tropical regions of Asia, Africa and
Australia (Ajibade et al. 2014).
A conspicuous semi-deciduous tree of coastal areas throughout the warm tropics,
Terminalia catappa grows best in most tropical climates. It is well adapted to sandy
and rocky coasts and flourishes on oolithic limestone. The natural habitat is in areas
just inland from ocean beaches, near river mouths and on coastal plains, but also
thrives when planted upland. It grows in regions on altitude 0–800 m, mean annual
temperature 15–35 °C and mean annual rainfall 750–3000 mm. The favorite soil
types include oolithic limestone, sands and loamy sands, silts, loam and clays. Soil
pH is usually neutral to moderately alkaline, rich in bases. It also grows on acidic
soil, but good drainage is required on clay soils (Orwa et al. 2009).
Methanolic extract contains steroids, triterpenes, triterpenoidal saponins, alkaloids,
flavonoids, tannins, glycosides and polyphenols (Babayi et al. 2004; Kankia 2012;
Praveena 2014; Behera et al. 2014). The fruit extract mainly contains aldehydes,
fatty acids, alkanols, ketones, esters, hydrocarbons and a heterocyclic compound.
Specifically, it contains cis-9-hexadecenal, 9Z-9-tetradecenal, palmitic acid, cis-9-
octadecenal, 6Z-6-octadecenoic acid, penta decenoic acid, arachidic acid,
1-(+)-ascorbic acid-2,6-dihexadecanoate (Ololade et al. 2014). Ikhwanuddin et al.
(2014) also reported the presence of the following tannins: punicalagin, punicalin,
terflavin A and B, tergallagin, tercatain, chebulagic acid, geranin, granatin B and
corilagin; flavonoids- isovitexin, vitexin, isoorientin, rutin and triterpenoids- ursolic
acid, 2a,3β,23-trihydroxy-urs-12-en-28-oic acid. Humic acid was also identified.
The tree is primarily used as an ornamental shade and salt-tolerant tree. The fruit is
eaten like almonds with similar oil (Praveena 2014). It has a bitter, acrid and astrin-
gent taste and is an aphrodisiac. The leaf juice is used as ointment for scabies,
leprosy and other cutaneous diseases (Nair and Chanda 2008). Terminalia catappa
has therapeutic effect for liver-related diseases, dermatitis and hepatitis (Praveena
2014). The leaf is used to treat rheumatic joints, cough, asthma, dysentery, diarrhea,
gonorrhea and skin ailments such as scabies (Behera et al. 2014). It is also a cardiac
stimulant.
Studies on the leaf and fruit have confirmed anticancer, antioxidant, anti-HIV
reverse transcriptase, anti-inflammatory, antidiabetic and hepatoprotective activities
(Praveena 2014). Anticancer, antioxidant, antifungal and anti-inflammatory proper-
ties have also been reported for different parts of the plant by Ikhwanuddin et al.
(2014).
The methanolic extract of Terminalia catappa has been shown to inhibit the
growth of two bacteria species, Bacillus subtilis and Escherichia coli at 200 mg/ml
and two fungal species, Aspergillus niger and Penicillium notatum at 200 mg/ml
(Praveena 2014). The organic extract also showed pronounced sensitivity against
Staphylococcus aereus and Klebsiella pneumoniae. It has a high antioxidant poten-
tial (Ololade et al. 2014). The methanolic extract of the leaf is considerably more
effective than the aqueous extract in inhibiting Gram positive, Gram negative bacte-
ria and a fungal strain (Nair and Chanda 2008). The petroleum ether, water and etha-
nolic fractions of leaf extract also showed significant antibacterial activity using
hole in plate bioassay procedure against Pseudomonas aeruginosa, Streptococcus
viridians and Staphylococcus aureus (Kankia 2012).
The growth inhibitory activity of the methanolic extract of Terminalia catappa
against Bacillus subtilis and Staphylococcus aureus has been reported by Babayi
et al. (2004). Akharaiyi et al. (2011) demonstrated that the aqueous leaf extract of
Terminalia catappa at different stages of maturity exhibited therapeutic effect on
different microorganisms, with the young leaf showing more potency than the
matured or red-pigmented leaf. The microorganisms studied include Bacillus cerius,
Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli. Others are
Proteus mirabilis, Salmonella typhi and Shigella dysenteriae.
Terminalia catappa has therapeutic effect against liver-related diseases, dermatitis
and hepatitis (Praveena 2014). The leaf is used in treatment of rheumatic joints,
cough, asthma, dysentery, diarrhea, gonorrhea and skin ailments such as scabies
(Behera et al. 2014), as well as a cardiac stimulant. The tree is primarily used as an
ornamental shade and salt-tolerant tree. The fruit is eaten like almonds with similar
oil (Praveena 2014). It has a bitter, acrid and astringent taste and is an aphrodisiac.
The leaf juice is used as ointment for scabies, leprosy and other cutaneous disease
(Nair and Chanda 2008).
7 Fabaceae
Detarium microcarpum Guill. & Perr.
Synonyms Detarium senegalense auct. non J.F.Gmel.


Detarium microcarpum Guill. (Fabacea) is an African leguminous medicinal plant.
Common Names Sweet Detar
(Source: www.prota4u.org, Ekpe/NSBP)
When found in dry areas Detarium microcarpum appear as small trees of about
10 m tall with a dense rounded crown, while in wet areas it can reach a height of
25 m. The greyish bark breaks off into rectangular pieces to reveal a reddish inner
surface. The twigs are covered with a smooth or peeling orange bark. The pinnate
leaves are 8–12 cm long and consist of 3–6 pairs of alternate, almost opposite leaf-
lets. The leaflets are 5–10 cm long and 3–5 cm wide, with a dull green upper surface
and a greyish-green lower surface. The leaflets have a rounded, often notched apex
and a rounded or subcordate base. The inflorescence is anaxillary raceme, of about
2–5 cm in length and congested. The creamy white fragrant flowers consist of
4 large sepals and 8–10 cream coloured prominent stamens, but petals absent
(Vautier et al. 2007).
Detarium microcarpum is distributed in the semi-arid sub-Saharan Africa, from
Senegal to Cameroon, extending eastwards to the Sudan. It has an irregular distribu-
tion and can be locally very common. The species is often left when farmland is
cleared. Typically, it is found in high rainfall savannah areas, dry forests and fallow
land, on sandy or iron rich hard soils. It also occurs in open savannah of Northern
Nigeria, parts of Chad and Niger republic as a more stunted tree with smaller fruits.
Detarium microcarpum grows on dry soil in wooded savanna and open woodland,
and is locally very common. It is most common in regions with an annual rainfall of
600–1000 mm. It is mainly found on shallow, stony and lateritic soils, and on hills.
Detarium microcarpum regenerates well from shoots produced by the trunk or
roots. Shoots from the trunk are much more vigorous than seedlings and can reach
a height of 1.5–2 m in 1–2 years. In Cameroon the average seedling height after 3
years is 0.6 m, and the seedling may reach 1.5 m in 4 years. Detarium microcarpum
flowers during the rainy season, from July–September (–November), and bears fruit
from September–January (–May). It sheds its leaves in November and produces
new ones in March. The main flowering period of a tree is up to 8 days only, and
flowers are pollinated by insects, especially in the mornings.
The seed gum contains D-galactose as a major monosaccharide, as well as
D-mannose and D-glucose. The fruit pulp contains 90% dry matter, of which 4–6
g/100 g protein and 3 mg/100 g ascorbic acid. The seeds yield 7.5% oil, with
linoleic acid being the predominant fatty acid. The gum content (water-soluble
polysaccharides) is high. The hulled seed flour contains per 100 g: water 3.5–6.5 g,
crude fiber 3 g, crude fat 13–15 g, crude protein 13.5–27 g, carbohydrate 39 g,
Ca 500 mg, Mg 500 mg, Fe 100 mg (Akpata and Miachi 2001).
Medicine
The roots, stems, bark, leaf and fruit are all used to treat ailments e.g. tuberculosis,
meningitis, itching and diarrhoea. The fruits and leaves are used traditionally in the
treatment of dysentery and syphilis. (Iwu 1993; Ikhiri and Ilagouma 1995) and the
root water extract is used for leprosy (Collier and Chapman 2001). The fruit is edi-
ble and rich in vitamin C and the leaves and seeds are also used in cooking. The
bark, leaves and roots of Detarium microcarpum are widely used throughout its
distribution area because of their diuretic and astringent properties. They are pre-
pared as infusions or decoctions to treat rheumatism, venereal diseases, urogenital
infections, haemorrhoids, caries, biliousness, stomach-ache, intestinal worms and
diarrhoea including dysentery (Burkill 1995). They are also used against malaria,
leprosy and impotence. A decoction of the powdered bark is widely taken to allevi-
ate pain, e.g. headache, sore throat, back pain and painful menstruation. The fresh
bark or leaf is applied to wounds, to prevent and cure infections.
In Mali the bark is also used to treat measles, nocturia, hypertension, itch and
tiredness, while a decoction of the leaves or roots is taken against paralysis, menin-
gitis, tiredness, cramps and difficult delivery. The powdered seeds are applied to
skin infections and inflammations, whereas the fruit is eaten to cure meningitis and
malaria. In Burkina Faso the fruit pulp is used for treating skin infections. A prepa-
ration of the fruits is taken against dizziness in Niger and Togo (Keay et al. 1958),
while in Senegal, a mixture of the leaf of Detarium microcarpum, Sclerocarya
birrea (A. Rich.) Hochst. and Acacia macrostachya Rchb. ex DC. pounded in milk
is considered very efficient for snakebites. In Benin a decoction of the leaf is taken
to treat fainting and convulsions, while generally in West Africa, the root is part of
a medico-magical treatment for mental conditions, and for protection against bad
spirits.
In traditional veterinary medicine, the leaf and root are used to treat diarrhoea in
cattle in southern Mali, and in Benin to treat constipation, while in Niger cattle
are made to inhale the smoke of the leaves to treat fever (Abreu et al. 1998).
Incorporation of 0.5% gum in wheat flour increased the water absorption and the
mixing tolerance index of the dough significantly. Oil-water emulsions stabilised by
the seed flour or gum tolerate freezing and thawing better than commercial salad
dressing, egg powder and gum tragacanth emulsions. Addition of the seed polysac-
charide to fruit products (mango, orange, pineapple, tomato) improved their stability
in storage (at 26 °C) for at least 2 months and was well acceptable to consumers.
Olugbuyiro et al. (2009) reported on anti-viral activity and cytotoxicity of D. micro-
carpium column fractions in the Huh-7 Replicon assay. The active fraction MTH-
1700 (6.155 g) which was eluted with EtOAc-MeOH (75:25) demonstrated good
inhibitory and selective potency (83.87%) against Hepatitis C virus in a dose depen-
dent manner compared with control (86.76%). The ethanolic extract of the bark
showed antimicrobial action against Pseudomonas aeruginosa, Citrobacter freun-
dii, Klebsiella pneumoniae, Staphylococcus aureus, Streptococcus pyogenes and
Listeria monocytogenes (Abreu et al. 1998). The extract also showed moderate anti-
tumour activity against breast cancer cells, while the flavanes present in the metha-
nolic extract of Detarium microcarpum showed strong inhibitory effects on HIV-1
or HIV-2 infection (Abreu et al. 1999). The stem bark extract has been demonstrated
to have significant molluscicidal activity against Lymnaea natalensis, probably
because it contains tetranorditerpenes, the clerodane diterpenes catechine and cis-2-
oxokolavenic acid (0.5%), the diterpene copalic acid (1.7%) and coumarin (1%).
A methanol extract of the leaf exhibited strong feeding deterrent activity against the
termite, Reticulitermes speratus.
8 Gramineae (Poaceae)
Bambusa vulgaris Schrad. ex J.C.Wendl.
(Source: E. Boer)

Bambusa vulgaris belongs to the subfamily Bambusoideae of Poaceae (Koshy and
Jee 2011).
Bambusa vulgaris is the most easily recognized species of all known bamboos
(Liese 2004). In a young stem, the primary branches are prominent; they are borne
alternately along the stem, together forming a gigantic fan-like structure which is
conspicuous from some distance. Bambusa vulgaris forms moderately loose clumps
and has no thorns. It has lemon-yellow culms (stems) with green stripes and dark
green leaves. Stems are not straight, not easy to split, inflexible, thick-walled, and
initially strong. The densely tufted culms grow 10–20 m (30–70 ft) high and
4–10 cm (2–4 in) thick. Culms are basally straight or flexuose (bent alternately in
different directions), drooping at the tips. Culm walls are slightly thick. Nodes are
slightly inflated. Internodes are 20–45 cm (7.9–17.7 in). Several branches develop
from mid-culm nodes and above. Culm leaves are deciduous with dense pubes-
cence. Leaf blades are narrowly lanceolate.
Bambusa vulgaris probably originated from tropical Asia, but grows throughout the
tropics and subtropics, including African countries like Nigeria, Senegal, Ghana,
Equatorial Guinea, Tanzania, and Cameroon.
Bambusa vulgaris grows best in tropical climate at lower altitudes; above 1000 m
altitude stems become smaller in length and diameter. It thrives under a wide range
of moisture and soil conditions, growing in almost permanently humid conditions
along rivers and lakes, but also in areas with a severe dry season, where the plants
may become completely defoliated.
Stem fibers from Bambusa vulgaris from tropical Africa (e.g. Côte d’Ivoire,
Cameroon, Gabon and Congo) have an average chemical composition of: cellulose
41–44%, pentosans 21–23%, lignin 26–28%, ash 1.7–1.9%, silica 0.6–0.7%. The
approximate chemical composition of young shoots per 100 g edible portion for
green-stem and yellow-stem cultivars respectively is: water 90 g and 88 g, protein
2.6 g and 1.8 g, fat 4.1 g and 7.2 g, carbohydrates 0.4 g and 0 g, fiber 1.1 g and 1.2 g,
Ca 22.8 mg and 28.6 mg, P 37 mg and 27.5 mg, Fe 1.1 mg and 1.4 mg, ascorbic acid
3.1 mg and 0 mg. Phytochemical screening of leaf extract of Bambusa vulgaris
revealed the presence of carbohydrate, glycosides, alkaloids, flavonoids, phytoster-
ols and triterpenoids (Kumar et al. 2011).
In Nigeria, a drink of macerated leaves is taken against venereal diseases, while in
DR Congo, the leaves form part of preparations used for treatment of measles. Other
ethno-medicinal usages include treatment of malaria fever, inflammations, ulcers
and wound healing. The roots are diuretic, tonic depurative, laxative and cooling and
also used in burning sensation, arthralgia, and general debility and dysuria. The
fruits are salutary in diabetes whereas, the seeds are useful in obesity to reduce fats.
Biological and Cytotoxic Activities
The antimalarial and antimicrobial potentials of the leaf and shoot have been dem-
onstrated. A chloroform extract of the leaf is active against Mycobacterium tubercu-
losis. Stigmasterol has been isolated from Arthrobacter globiformis fermented
shoot (Sarangthem et al. 1998). In a study (Ojo et al. 2009) comparing the antiviral
potentials of Nigerian plant, B. vulgaris resulted in inhibition only on measles virus
at MIC of 62.5 μg/ml, justifying the usage of the plant in traditional medicine to
treat measles.
The shoots of B vulgaris contains taxiphyllin (a cyanogenic glycoside) that func-
tions as an enzyme inhibitor in the human body when released, but degrades readily
in boiling water. It is highly toxic, and the lethal dose for humans is about 50–60 mg.
A dose of 25 mg cyanogenic glycoside fed to rats (100–120 g body weight) caused
clinical signs of toxicity, including apnoea, ataxia and paresis (Satya et al. 2010).
Ingestion of large amounts of leaf has been recorded to cause neurological disor-
ders in horses, but this was not related to poisoning by hydrocyanic acid (Barbosa
et al. 2006).
Ethno-medicinal usages include treatment of malaria fever, inflammations, ulcers
and wound healing. The antimalarial and antimicrobial potential of the leaf and
shoots have been reported.
9 Lauraceae
Cinnamomum verum J. S. Presl.
Synonyms Cinnamomum zeylanicum Nees
(Source: Steven Foster)


Cinnamomum verum J.S. Presl. (family: Lauraceae) is commonly called the
cinnamon tree.
Morphology
Cinnamomum verum J.S. Presl. is an evergreen tree that reaches a height of 8–17 m
in the wild. In an un-harvested state, the trunk is stout, 30–60 cm in diameter, with
a thick, grey bark and the branches set low down. Leaves are stiff, estipulate, oppo-
site and somewhat variable in form and size.
Petiole is 1–2 cm long, grooved on the upper surface. The lamina is usually
5–18 × 3–10 cm, ovate or elliptic; base more or less rounded and the tip tends to be
somewhat acuminate. There are 3, sometimes 5, conspicuous longitudinal veins
found at the base of the lamina and running almost to the tip. The young leaves of
the flush are reddish, later turning dark green above with paler veins and pale glau-
cous beneath. Flowers are borne in lax axillary and terminal panicles on the ends of
twigs. Peduncles are creamy white, softly hairy, 5–7 cm long. Individual flowers are
very small, about 3 mm in diameter, pale yellow, with a foetid smell, each subtended
by a small, ovate, hairy bract. The calyx is campanulate and pubescent with 6
acutely pointed segments. Corolla is absent. Fruit is a fleshy ovoid drupe, black,
1.5–2 cm long when ripe, with the enlarged calyx at the base (Orwa et al. 2009).
Geogaphical Distribution
Cinnamomum verum J.S. Presl. is indigenous to Sri Lanka. It also grows in India,
Brazil, Colombia, Comoros, Dominica, Fiji, Ghana, Haiti, Indonesia, Jamaica,
Madagascar, Malaysia, Mauritius, Mexico, Nicaragua, Nigeria, Philippines, Puerto
Rico, Seychelles, Sierra Leone, Tanzania and Uganda.
Cinnamomum verum requires a warm and wet climate with no extremes of tempera-
tures. Although there can be months in which there is less rain, no prolonged dry
season should occur and rain received should be about 150 days per year. Rocky and
stony ground is unsuitable, while waterlogged and marshy areas should be avoided,
as they result in an undesirable, bitter product, which is much less aromatic. An
altitude below 500 m, mean annual temperature of 27 °C, mean annual rainfall of
over 2000 mm are required. It is tolerant to a wide range of soils.
Cinnamomum verum contains a wide range of essential oils, such as trans
cinnamaldehyde, cinnamyl acetate, eugenol, L-borneol, caryophyllene oxide,
b-caryophyllene, L-bornyl acetate, E-nerolidol, -cubebene, -terpineol, terpinolene,
and -thujene (Rao and Gan 2014). Flavonoids, saponins, terpenoids and cardiac
glycosides are also present (Udaya Prakash et al. 2014).
Cinnamon verum bark is used as natural food preservative, preventing the decom-
position of products due to its rich content of cinnamaldehyde which has been
proven to be active against many pathogenic gram positive and gram negative bac-
teria (Asha et al. 2014). It is used as a flavouring agent in foods and chewing gum.
Cinnamon is also used to improve the health of the colon thereby reducing the risk
of colon cancer. It is a coagulant and prevents bleeding (Rao and Gan 2014).
Evaluation of essential oil of Cinnamomum verum and its major component cinna-
maldehyde on growth, hyphal ultrastructure and virulence factors of Aspergillus
fumigatus and Trichophyton rubrum, showed potent antifungal activity on both liq-
uid and solid media. Further test also revealed inhibitory activity against germinated
and un-germinated conidia in test fungi. Electron microscopic studies also revealed
multiple sites of action of cinnamaldehyde mainly on cell membranes and endo-
membranous structures of the fungal cells. The essential oil and cinnamaldehyde
also caused a 70% reduction in elastase activity in Aspergillus fumigatus (Mohammed
and Igbal 2011).
At 500 mg/ml Cinnamomum verum aqueous extract completely inhibited
Aspergillus flavus, confirming a fungicidal activity that can make it an alternative to
synthetic fungicides (Lakshmeesha et al. 2014).
Mayaud et al. (2008) also tested the essential oils containing aldehydes from
Cinnamomum verum bark and phenols from the leaves for antimicrobial activity
against 55 bacterial strains, using two methods, agar dilution and time-killing curve
methods. Results showed that the Cinnamomum verum bark had the highest antimi-
crobial activity, particularly against resistant strains. In a study to evaluate the role
of Cinnamomum verum essential volatile oil extracts on human macrophages
infected by Brucella abortus 544, the oil at a concentration of 1% had the highest
antibacterial activity against B. abortus 544 inside human macrophages (Al-Mariri
et al. 2012). Other biological activities documented for cinnamon include antioxi-
dant, antidiabetic, anti-inflammatory, antiemetic, antimycotic and anticancer activi-
ties (Rao and Gan 2014).
Cinnamon bark oil is employed in dental and pharmaceutical preparations. The
drops are used as a tonic, a sedative in childbirth and a remedy for many common
disorders. It is used for gastrointestinal and respiratory disorders and as an aphrodi-
siac. It is also used to relieve headache, speed up parturition, as a carminative and to
treat catarrh. The bark extract is an intestinal astringent and the essence is used as a
poultice for rheumatism and is taken orally for spasms and for stomach and intesti-
nal gas (Orwa et al. 2009).
10 Conclusion
This review has shown that many wild Nigerian trees belonging to different families
are widely used in traditional medicine, and have indeed been demonstrated to have
several biological and therapeutic effects due the presence of a variety of phyto-
chemicals. The need to establish methods of domesticating them by cultivation as a
means of conservation and sustainability for the present and future generations can-
not be over-emphasized.
Acknowledgements Some of Professor S.E. Atawodi’s research work was partly supported by

Alexander von Humboldt Foundation of Germany and the German Cancer Research Centre
(DKFZ), Heidelberg.
References
Abreu PM, Rosa VS, Araujo EM, Canda AB, Kayser O, Bindseil KU, Siems K, Seemann A (1998)
Phytochemical analysis and antimicrobial evaluation of Detarium microcarpium bark extracts.
Pharm Pharmacol Lett 8(3):107–109
Abreu PM, Martins ES, Kayser O, Bindseil KU, Siems K, Seemann A, Frevert J (1999)
Antimicrobial, antitumor and antileischmania screening of medicinal plants from Guinea-
Bissau. Phytomed 6(3):187–195
Adewole SO, Caxton-Martins EA (2006) Morphological changes and hypoglycemic effects
of Annona muricata Linn. (Annonaceae) leaf aqueous extract on pancreatic β-cells of
streptozotocin-treated diabetic rats. Afr J Biomed Res 9:173–187
Afolabi F, Afolabi OJ (2013) Phytochemical constituents of some plants in Southwest Nigeria.
IOSR J Appl Chem 4(1):76–78
Ajibade VA, Ibikunle KSO, Owoseni DO (2014) Incidence, multidrug resistance and antimicrobial
activity of extracts from Terminalia catappa on Streptococcus pyogenes. J Basic Appl Sci Res
4(4):6–10
Akharaiyi FC, Ilori RM, Adesida JA (2011) Antibacterial effect of Terminalia catappa on some
selected pathogenic bacteria. Int J Pharm Biomed Res 2(2):64–67
Akinyemi OI, Dada EO (2013) Phytochemical screening and antityphoid properties of ethanolic
leaf extract of Parquetina nigrescens. ARPN J Agric Biol Sci 8(11):732–739
Akpata MI, Miachi OE (2001) Proximate composition and selected functional properties of
Detarium microcarpum. Plant Fd Hum Nutr 56(4):297–302
Akunyili DN, Houghton PJ (1993) Monoterpenoids and napthoquinones from Kigelia pinnata
bark. Phytochemistry 32:1015–1018
Akunyili DN, Houghton PJ, Raman A (1991) Antimicrobial activities of the stem bark of Kigelia
africana. J Ethnopharmacol 35:173–178
Alamelu I, Bhuwan CJ (1974) An iridoid glycoside from Kigelia pinnata. Herba Pol 20:319
Al-Mariri A, Saour G, Hamou R (2012) In vitro antibacterial effects of five volatile oil extracts
against intra macrophage Brucella abortus 544. Iran J Med Sci 37(2):119–125
Alvarez Cruz NS (2012) Parquetina nigrescens (Afzel.) Bullock. In: Schmelzer GH, Gurrib-
Fakim A (eds) Prota 11(2): medicinal plants/Plantes medicinales 2. PROTA, Wageningen,
Netherlands. Accessed 6 May 2015
Arbab AH (2014) Review on Anogeissus leiocarpus a potent African traditional drug. Int J Res
Pharm Chem 4(3):496–500
Asha S, Nithisa K, Bharath Kumar R, Ravi KV (2014) Deciphering the antimicrobial potential of
Cinnamon zealanicum bark. J PharmTech Res 6(4):1226–1235
Atawodi SE (2005) Antioxidant potential of African medicinal plants – a review. Afr J Biotechnol
4(2):128–133
Atawodi SE (2011) Evaluation of the hypoglycemic, hypolipidemic and antioxidant effects of
methanolic extract of “Ata-ofa” polyherbal tea (A-Polyherbal) in alloxan-induced diabetic rats.
Drug Inv Today 3(11):270–276
Atawodi SE, Olowoniyi OD (2015) Pharmacological and therapeutic activities of Kigelia africana
(Lam.) Benth. Ann Res Rev Biol 5(1):1–17
Atawodi SE, Adekunle OO, Bala I (2011) Antioxidant, organ protective and ameliorative prop-
erties of methanol extract of Anogeissus leiocarpus StemBark against carbon tetrachloride-
induced liver injury. Int J Pharm Sci Res 2(6):748–753
Atawodi SE, Yakubu OE, Umar IA (2013) Effect of methanolic extract of root of Parinari curatel-
lifolia Planch. Ex benth on acute and chronic oxidative stress and hepatotoxicity induced by
carbon tetrachloride in rats. Int J Agric Biol 15:523–528
Atawodi SE, Olowoniyi OD, Adejo GO, Liman ML (2014a) Review of the antioxidant potential
of African medicinal plants and food plants. In: Dubey NK (ed) Plants as a source of natural
antioxidants. CAB International, Wallingford. 63pp
Atawodi SE, Olowoniyi OD, Obari MA, Ogaba I (2014b) Ethnomedical survey of Adavi and
Ajaokuta local government areas of Ebiraland, Kogi State, Nigeria. Ann Res Rev Biol
4(24):4344–4360
Atawodi SE, Olowoniyi OD, Daikwo MA (2014c) Ethnobotanical survey of plants used for the
management of Hypertension in the Igala speaking area of Kogi State, Nigeria. Ann Res Rev
Biol 4(24):4535–4543
Atolani O, Olatunji AG (2010) Epicuticular wax and volatiles of Kigelia pinnata leaf extract.
Ethnobotan Leaflets 14:79706
Attiouaaf B, Yeo LLD, Antheaume C, Kaiser M, Weniger B, Lobstein A, Vonthron-Sénécheau C
(2011) In vitro antiplasmodial and antileishmanial activities of flavonoids from Anogeissus
leiocarpus (Combretaceae). Int J Pharm Sci Rev Res 11(2), Article-001, pp 1–6
Babayi H, Kolo I, Okogun JI, Ijah UJJ (2004) The antimicrobial activities of methanolic extracts of
Eucalyptus camaldulensis and Terminalia catappa against some pathogenic microorganisms.
Biok 16(2)
Barbosa JD, de Oliveira CMC, Duarte MD, Riet-Correa G, Peixoto PV, Tokarnia CH (2006)
Poisoning of horses by bamboo, Bambusa vulgaris. J Eq Vet Sci 26(9):393–398
Barku Y, Victor A, Abban G (2013) Phytochemical studies, in-vitro antibacterial activities and
antioxidant properties of the methanolic and ethyl acetate extracts of the leaves of Anogeissus
leiocarpus. Internl J Biochem Res Rev 3(2):137–145
Behera DR, Bhatnagar S, Mahapatra AK (2014) Cytotoxic and radical scavenging potential of
Indian Almond (Terminalia catappa) leaf extracts. Br Biomed Bull 2(1):31–39
Bharti N, Singh S, Naqvi F, Azam A (2006) Isolation and in vitro antiamoebic activity of iridoids
isolated from Kigelia pinnata. ARKIVOC 2006:69–76
Borokini IB, Okere AU, Giwa AO, Daramola BO, Odofin WT (2010) Biodiversity and Consevation
of plant genetic resources in field Genebank of the National Center for Genetic Resources and
Biotechnology, Ibadan, Nigeria. Int J Biodiv Conserv 2(3):037–050
Burkill HM (1985) The useful plants of West Tropical Africa, vol 1, 2nd edn. Royal Botanical
Gardens, Kew
Burkill HM (1995) The useful plants of West Tropical Africa. 2nd edition. Volume 3, Families
J–L. Royal Botanic Gardens, Kew/Richmond, 857 pp
Chidumayo EN, Gumbo DJ (2010) The dry forests and woodlands of Africa: managing for prod-
ucts and services. Earthscan Publisher, Quebec
Collier J, Chapman R (2001) Combination therapy with interferon-alpha and ribavirin for hepatitis
C: practical treatment issues. BioDrugs 15(225):238
Fasola TR, Okeocha PC, Odetola A (2010) Screening for the hypoglycaemic potentials of the
extract of Vernonia amygdalina. Ethnobotan Leaflets 14:759–765
Feitosa EA, Xavier HS, Randau KP (2012) Chrysobalanaceae: traditional uses, phytochemistry
and pharmacology. Rev Bras Farm 22(5):1181–1186
Gouda Y, Abdel-baky A, Darwish F, Mohammed K, Kesai R, Yamasaki K (2003) Iridoids from
Kigelia pinnata, DC. Phytochemistry 63:887–892
Govindachari TR, Patankar SJ, Vishvanthan N (1971) Isolation and structure of two new dihy-
droisocumarins from Kigelia pinnata. Phytochemistry 10:1603–1606
Grace OM Davis S (2002) Kigelia africana (Lam) Benth. Record from Protabase. In: Oyen LPA,
Lemmens RHMJ (eds) PROTA (Plant Resources of Tropical Africa, Wageningen, Netherlands).
http://database.prota.org/search.htm
Harouna S, Adama H, Djifaby SPAE, Moussa C, Roland M, Millogo J, Nacoulma OG (2012)
Phytochemistry and biological activities of extracts from two Combretaceae found in
BurkinaFaso: Anogeissus Leiocarpus (DC) Guill. and Perr. and Combretum Glutinosum Perr.
Ex DC. Univers J Environ Res Technol 2(5):383–392
Hennenberg JF, Goetze D, Minden V, Traoré D, Porembski S (2005) Size-class distribution of
Anogeissus leiocarpus (Combretaceae) along forest–savanna ecotones in northern Ivory Coast.
J Trop Ecol 21:273–281
Houghton PJ, Akunyili DN (1993) Iridoids from Kigelia pinnata bark. Fitoterapia 65:473–474
Ikhiri K, Ilagouma AT (1995) Fitoterapia 46:274–274
Ikhwanuddin M, Moh JHZ, Hidayah M, Hidayati ABN, Aina-Lyana NMA, Juneta ASM (2014)
Effect of Indian almond Terminalia catappa leaves water extract on the survival rate and growth
performance of black tiger shrimp Penaeus monodon post larvae. Aquaculture, aquarium, con-
servation and legislation. Int J Bioflux Soc 7(2):85–93
Imaga NA (2013) Phytomedicines and nutraceuticals: alternative therapeutics for sickle cell ane-
mia. Sci World J 2013:1–12
Iwu MM (1993) Handbook of African medicinal plants. CRC Press Inc, London
Jackson S, Beckett K (2012) Sausage tree Kigelia pinnata: an ethnobotanical and scientific review.
HerbalGram 94:48–59
Jayaweera DMA (1982) Medicinal plants (indigenous and exotic) used in Ceylon, vol IV. The
National Science Council of Sri Lanka, Colombo
Kankia HI (2012) Phytochemical screening and antibacterial activities of leaf extracts of Terminalia
catappa (Umbrella tree). Int J Sci Res 3(12):2658–2661
Keay RWJ, Hoyle AC, Duvigneaud P (1958) Caesalpiniaceae. In: Keay RWJ (ed) Flora of West
Tropical Africa. Volume 1, Part 2, 2nd edn. Crown Agents for Oversea Governments and
Administrations, London, pp 439–484
Koshy KC, Jee G (2011) Studies on the absence of seed set in Bambusa vulgaris. Curr Sci
81(4):375–378
Kumar M, Krishnamurth S, Suseel S (2011) Phytochemical investigation and acute toxicity study
of methanol extract of Bambusa vulgaris leaves. J Pharm Res 4(2):403
Kupeli E, Harput U, Varel M, Yesilada E, Saracoglu I (2005) Bioassay guided isolation of iridoid,
glucosides with antinociceptive and anti-inflammatory activities from Veronica anagallis-
aquatic L. J Ethnopharmacol 102:170–176
Lakshmeesha TR, Sateesh MK, Vedashree S, Sofi MS (2014) Antifungal activity of Cinnamomum
verum on soybean seed-born Aspergillus flavus. Int J Adv Res 2(5):1169–1172
Liese W (2004) Preservation of bamboo structures. Gh J For 15–16:40–48
Maharaj V, Glen HF (2008) Parinari curatellifolia, KZN Herbarium, South African National
Biodiversity Institution Plant Information Website
Mayaud L, Carricajo A, Zhiri A, Aubert G (2008) Comparison of bacteriostatic and bacteriocidal
activity of 13 essential oils against strains with varying sensitivity to antibiotics. Lett Appl
Microbiol 47(3):167–173
Mishra S, Ahmad S, Kumar N, Sharma BK (2013) Annona muricata (the cancer killer): a review.
Glob J Pharmacet Res 2(1):1613–1618
Mohammed SAK, Igbal A (2011) In vitro antifungal, anti elastase and anti keratinase activity of
essential oils of Cinnamomum, Syzygium and Cymbopogon species against Aspergillus fumiga-
tus and Trichophyton rubrum. Phytomed 19(1):48–55
Nair R, Chanda S (2008) Antimicrobial activity of Terminalia catappa, Manilkara zyota and Piper
betel leaf extracts. Indian J PharmaceutSci 70(3):390–393
Odetola AA, Oluwole FS, Adeniyi BA, Olatiregun AM, Ikupolowo OR, Labode O, Busari KO,
Shorinola JA (2006) Antimicrobial and gastrointestinal protective properties of Parquetina
nigrescens (Afzel.) Bullock. J Biol Sci 6(4):701–705
Ojo OO, Oluyege JO, Famurewa O (2009) Antiviral properties of two Nigerian plants. African
J Plant Sci 3(7):157–159
Olatunji AG, Atolani O (2009) Comprehensive scientific demystification of Kigelia africana; a
review. Afr J Pure Appl Chem 3(9):158–164
Ololade ZS, Olatunde OZ, Oyelese OJ, Olaoye OO, Odewande AA (2014) Total phenolic content,
free radical inhibition, antioxidant and antibacterial potential of the medicinal organic com-
pounds in the fruit of Terminalia catappa Linn. Nat Sci 12(2):46–50
Olugbuyiro JAO, Moody JO, Hamann MT (2009) Inhibitry effect of Detarium microcarpum
extract against hepatitis C virus. Afr J Biomed Res 12:2
Onwuliri FC, Mawak ID, Wonang DL, Onwuliri EA (2006) Phytochemical, toxicological, toxico-
logical and histopathological studies of some medicinal plants in Nigeria. Internl J Nat Appl
Sci 2(3):225–229
Orwa C, Mutua A, Kindt R, Jamnadars R, Anthony S (2009) Agroforestree database: a tree refer-
ence and selection guide version 4.0. http://www.worldagroforestry.org/sites/treedbs/treedata-
bases.asp. Accessed on the 5 May 2015
Owolabi OJ, Omogbai EKI, Obasuyi O (2007) Antifungal and antibacterial activities of the etha-
nolic extract of Kigelia africana (Bignoniceae) stem bark. Afr J Biotechnol 6(14):1677–1680
Peni IJ, Elinge CM, Yusuf H, Itodo AU, Agaie BM, Mbongo AN, Chogo E (2010) Phytochemical
screening and antibacterial activity of Parinari curatellifolia stem extract. J Med Plant Res
4(20):2099–2102
Praveena K (2014) Phytochemical, antimicrobial and in vitro antioxidant activity of Terminalia
catappa. Int J Pharm Life Sci 5(2):3325–3329
Rao PV, Gan SH (2014) Cinnamon: a multi-faceted plant. Evid Based Complement Alternat Med
Article ID 642942
Rivera JO, Loya AM, Cabalios R (2013) Use of herbal medicines and implications for conventional
drug therapy medical sciences. Altern Integ Med 2:130. doi:10.4172/2327-5162.1000130
Saini S, Kaur H, Verma B, Ripudaman T, Singh S (2009) Kigelia africana (Lam.) Benth. An over-
view. Nat Prod Rad 8(2):190–197
Sarangthem K, Singh LJ, Srivastava RC (1998) Isolation of stigmasterol from fermented succulent
bamboo shoots and its biotransformation into ADD using Arthrobacter globiformis. Indian
J Plant Physiol 3(4):249–252
Satya S, Bal LM, Singhal P, Naik SN (2010) Bamboo shoot processing: food quality and safety
aspect (a review). Trends Food 21:181–189
Singh P, Khandelwal P (2010) Cetyltriacontanoate and other constituents from Acacia jacquemon-
tii and Kigelia pinnata. J Indian Chem Soc 87:1403–1407
Sulaiman H, Roslida AH, Fezah O, Tan KL, Tor YS, Tan CI (2012) Chemopreventive potential of
Annona muricata L leaves on chemically-induced skin Papillomagenesis in mice. Asian Pac
J Cancer Prev 13:2533–2539
Tyagi A, Singh V, Bharadwaj M, Kumar A, Thakur K (2011) Isolation and antibacterial suscepti-
bility testing of multi-drug resistant Pseudomonas aeruinosa causing urinary tract infection.
J Chem Pharm Res 3(4):342–347
Udaya Prakash NK, Bhuvaneswari S, Sripriya N, Arulmozhi K, Kavitha K, Aravitha R, Bharathiraja
B (2014) Studies on phytochemistry, antioxidant, antibacterial, larvicidal and pesticidal activi-
ties of aromatic plants from Yelagiri Hills. Int J Pharm Pharm Sci 6(5):325–328
Vautier H, Sanon M, Sacande M (2007) Detarium microcarpum Guill. & Perr. Seed Leaflet 122
Victor Y, Barku A, Opoku-Boahen Y, Owusu-Ansah E, Dayie NTKD, Mensah FE (2013) In-vitro
assessment of antioxidant and antimicrobial activities of methanol extracts of six wound heal-
ing medicinal plants. J Nat Sci Res 3(1):74–80
Watt JM, Breyer-Bradwijk MG (1962) The medicinal and poisonous plants of Southern and
Eastern Africa, 2nd edn. Livingstone, London, p 52
Wurochekke AU, Eze HT, Nelson K (2013) Effect of methanolic leaf extract of Parinari curatel-
lifolia on rat liver and kidney. Sky J Biochem Res 2(7):47–49
Biological, Pharmacognostic
and Phytochemical Review of Some
Cultivated Medicinal Plants of Nigeria
Sunday E. Atawodi, Godwin O. Adejo, Olufunsho D. Olowoniyi,

Abstract Medicinal plant cultivation is more of a rarity than common in Nigeria,

similar to other areas in Africa, where these unquantifiable resources are rather
shoddily harvested from the wild. This review identifies five cultivated medicinal
plants species of Nigeria, i.e.: Vernonia amygdalina Del, Cyperus esculentus,
Cymbopogon citratus Stapf, Khaya senegalensis (Desv.) A. Juss and Moringa oleif-
era Lam with purported biological and pharmacognostic effects and relates these
effects to their phytochemical constituents, where possible. The findings are
arranged according to general aspects, cultivation practices, post-harvest handling,
production schedules and utilization are discussed. There appears to be no available
information on Khaya senegalensis (Desv.) A. Juss. with respect to cultivars, grad-
ing, packing and storage. The low number and lack of comprehensive literature on
cultivated medicinal plants, seems to be an indication of a near or total lack of atten-
tion or interest on the issue of medicinal plant conservation in Nigeria, and hence,
this contribution could be regarded a wake-up call.
Keywords Cultivated • Medicinal plants • Phytochemicals • Pharmacognosy •

Therapeutics • Potential • Herbal medicine • Nigeria
1 Introduction
The utilization of medicinal plants in preventing, managing and treating diseases

play cardinal roles in most parts of the developing world, including Nigeria (Atawodi
et al. 2009a, b; Ene et al. 2009). Of particular significance is the use of herbal plants
or those regarded as medicinal food plants, which are vastly used as the primary
Dedicated to Our Students and Mentees for additional impetus towards continuous scientific
contributions
S.E. Atawodi (*) • G.O. Adejo • O.D. Olowoniyi • M.L. Liman

DOI 10.1007/978-94-024-1120-1_12
health care agents in rural areas where access to medical services are not readily
available, accessible or affordable (Liman and Atawodi 2015). Surprisingly, despite
the depletion of these forest resources due to factors ranging from over exploitation,
bush burning, desert encroachment and drought, there exist great paucity in the
inclination of the Nigerian farming population to cultivate these plants. It has indeed
been reported elsewhere that the majority of plants with medicinal values are uncul-
tivated (Mpondo and Dibong 2011) and simply collected from the wild.
A survey of cultivated and uncultivated medicinal plants carried out in Kwardu,
Churkah Shigar and Tormik villages of the Himalayan mountain watershed showed
that only 27%, 33% and 31% respectively, were cultivated (Raza and Shah 2011).
Similarly, Getaneh and Girma (2014) have put the statistics at 75% for uncultivated
and 25% for cultivated plants. A search through the literature showed that only a
few plants with medicinal values are cultivated in Nigeria, the notable ones of which
are here presented.
It is quite noticeable from the foregoing that cultivated medicinal plants are tre-
mendously few, although possibility exists that larger numbers of these plants are
cultivated in Nigeria, but are not documented. Therefore, this work was set to
achieve two goals. First, to identify the cultivated medicinal plants in Nigeria and
secondly, to glean through existing documented literature and build more on such
plants’ biological, pharmacognostic and phytochemical properties. Exposures aris-
ing from this piece could inform relevant stakeholders to take advantage of the huge
gap that exist and provide opportunities for sustained harvests of useful medicinal
plants, contribute to local needs on traditional medicines, highlight potential eco-
nomic benefits, and contribute to the climatic change salvaging drive, as well as
conservation efforts.
Asteraceae
Vernonia amygdalina Del.
Vernonia amygdalina Del. is a plant of medicinal importance which even though

found in the wild, is also well cultivated for its useful culinary and medicinal values
(Dansi et al. 2008). Because of its high requirement for water, it grows mainly dur-
ing the rainy season and on irrigated soils. This mono-cropped or inter-row cropped
plant, mostly at the backyard is propagated mainly by root cutting. The leaf is the
most useful part in its fresh form, although mostly stored in the dried form. The leaf
is highly used in food flavoring, while research has shown that V. amygdalina pos-
sess diverse pharmacological properties, including being an active hypoglycaemic,
anticancer (Akah and Okafor 1992; Agbogidi and Akpomorine 2013) and immune
fortifying agent.
Biological, Pharmacognostic and Phytochemical Review of Some Cultivated Medicinal… 313
Vernonia amygdalina, Bitter leaf (Photo by authors)
Part 1: General Aspects
Vernonia amygdalina Del. occurs as shrub or small tree, about 10 m tall, branched;
trunk up to 40 cm in diameter; bark grey to brown, smooth, becoming fissured;
young branches densely pubescent. Leaves are alternate, simple; stipules absent;
petiole 0.2–4 cm long; blade ovate-elliptical to lanceolate, 4–15(−28) cm × 1–4
(−15) cm, cuneate or rounded at base, shortly acuminate at apex, margin minutely
toothed to coarsely serrate, finely pubescent but often glabrescent and pinnately
veined. Inflorescence at head, arranged in terminal, compound, umbel-like cymes;
stalk of head up to 1 cm long, pubescent; involucre cylindrical to broadly ellipsoid,
3–5 mm long, bracts 3–7-seriate, 1–4.5 mm long, appressed. Flowers bisexual, reg-
ular, 5-merous, strongly exerted from the involucre; pappus consisting of outer lin-
ear, caduceus scales up to 1.5 mm long and of inner creamy or brownish bristles
4–7 mm long; corolla tubular, 5–8 mm long, whitish, glandular, with erect lobes;
stamens with anthers united into a tube, with appendages at apex; ovary inferior,
1-celled, pubescent and glandular, style hairy, 2-branched. Fruit occurs as a
10-ribbed achene 1.5–3.5 mm long, pubescent and glandular, brown to black,
crowned by the much longer pappus bristles, while seedling occurs with epigeal
germination (Ucheck 2004).
Classification
Vernonia amygdalina Del.family of Asteraceae [Compositae] (Ucheck 2004;
Yedjou et al. 2008; Agbogidi and Akpomorine 2013).
Origin and Distribution
Widespread in East, Central and West African countries, including Nigeria (Yedjou
et al. 2008).
Soil Requirements
They are usually planted in the morning, in loamy soil, at a depth of 2–5 cm deep
(Agbogidi and Akpomorine 2013).
Production Levels
Vernonia amygdalina is a relatively short cycle crop that is harvested twice in a
month, for up to 7 years. As the leaf is the material of interest, the leaves are usually
harvested before onset of flowers as the yield of leaves drop sharply at this point up
to time of seeds setting and seed maturation (Dansi et al. 2008). However, the high-
est yields are obtained during the rainy season, the peak being in May–August
(Ucheck 2004).
Major Production Areas in Africa
It is grown in many countries in savannah zones and popular in most of West Africa
countries including, Nigeria, Cameroon, Gabon and Congo Democratic Republic
(Agbogidi and Akpomorine 2013), where they are also cultivated in the backyard of
most households in the rural areas.
Cultivars
Vernonia amygdalina has two main cultivars which are the bitter and non-bitter.
Chromosome numbers and karyotype in three species of the genus Vernonia
Schreber in Southern Nigeria (Kemka-Evans and Okoli 2013).
Part 2: Cultivation Practices
Propagation
Although it grows nearly everywhere, propagation is mainly by cutting. The stems
of various length of V. amygdalina are cut in the afternoon, soothed and tied in
bundles. The bundles are kept overnight, placed upright in a basin of water or
covered with jute bag to avoid desiccation. They are now planted in the morning on
loamy soil at a depth of 2–5 cm deep (Agbogidi and Akpomorine 2013).
Soil Preparation
Preparation of land for V. amygdalina planting is done with hand held hoes (Dansi
et al. 2008). When propagated through the seed, planting is usually through broad-
casting, and localized in portions of fields with debris from burnt grasses or wastes.
Nurseries are also used to raise seedlings which are later transplanted on the fields.
Although, the practice is rare among households (Dansi et al. 2008), it is most likely
done among commercial vegetable farmers.
Planting/Fertilization
Vernonia amygdalina like many traditional leafy vegetables are planted mostly
around or near homes, gardens, cattle enclosures, fertile portions of the cropland,
and near riverbanks and swamps. Cultivation within close proximities to human
abodes or easily accessible locations is mainly for protection from thieves, birds and
animals, enriching soil fertility from dumping of refuse from kitchen to enhance
yields (Dansi et al. 2008), to receive water from domestic use and to maintain fresh-
ness. The systems of cropping in the cultivation of V. amygdalina are mixed, inter-
row, and mono-cropping. However, the most common cropping systems practiced
are the mixed and inter-row cropping (Dansi et al. 2008).
Irrigation
Some farmers grow V. amygdalina near rivers and in swamps for easy irrigation
especially during draught and mostly by those who produce in commercial quanti-
ties (Dansi et al. 2008).
Weed Control
Weed control among farmers of V. amygdalina is usually by tillage, which is manu-
ally carried out since the use of tractor and associated implements in tillage opera-
tions are beyond their reach (Nmor and Odeh 2013).
Pest and Disease Control
No major diseases affect the production of V. amygdalina apart from leaf curl virus.
Pests do not cause major damage either, although many pest species have been
recorded on bitter leaf in northern Nigeria like thrips, aphids, ants, white fly,
Empoasa spp., Sphearocoris annulus, Fabricius spp., Ptyelus grossus, Polyclaeis
spp. and Xanthochelus vulneratus. To control these, people traditionally sprinkle
wood ash on the leaves to keep ants and aphids away. The bitter leaf weevil Lixus
camerunus may damage stems and branches by making tunnels, causing branches
to break (Ucheck 2004).
Harvesting
The young green leaves are plucked from the plant by hand or cut with a knife.
Part 3: Post-harvest Handling
Part Collected and Collection Techniques Used

The leaves are usually put to use in traditional medicine and as hops to make beer,
where the tops of the shrubs are collected. In most other uses, the broad mature
green leaves are collected. For human consumption, leaves are squeeze-washed or
boiled to help remove the extreme bitterness and foam (Aregheore 1998).
Grading
The broad green leaves could be macerated and used as vegetables or condiments in
cooking soup. In other instances, the water extract is collected and used as tonic for
prevention of certain ailments. The bitter leaves are washed in some climes and
preserved by drying under the sun or by freezing (Aregheore et al. 1998).
Packaging
Stems of various lengths are cut in the afternoon then sorted and tied into bundles of
roughly equal length. Depending on the season, stems brought to the market have a
length of 30–50 cm, often longer during the peak period. Bundles of 15–20 stems
weighing 1–2 kg are often made, but smaller ones are made during periods of scar-
city. The bundles are kept overnight, placed upright in a basin of water and some-
times covered with jute bags to avoid desiccation. The bundles themselves are tied
together into bigger bundles before they are carried to the market. During dry peri-
ods people pick only the leaves and leave the shoots intact (Ucheck 2004).
Storage
Vernonia amygdalina is rarely stored because it is grown only enough to meet
household consumption needs. It also has low storage capacity being perishable in
the fresh form. As such, even in commercial cultivation, they are sold soon after
harvest in order to cut losses. However, some growers of V. amygdalina, dry the
harvested leaves whole or chopped, under the sun. Recently, there is more of shade-
drying in an airy environment and storage in traditional containers such as gourds.
Shade-drying reduces blanching, although blanching improves colour and caro-
tene retention due to inactivation of enzymes. However, it results in ascorbic acid
loss (Chweya and Eyzaguirre 1999). Reed mats or sacks are the common drying
surfaces, which are placed on raised platforms or roof tops in order to protect veg-
etables from domestic animals and soil. However, the leaves are susceptible to
infestation by insects and moulds. In some places, the leaves are spread on the
ground and naturally occurring flat stone surfaces, where these are found (Dansi
et al. 2008).
Marketing
According to (Dansi et al. 2008), the selling of vegetables, including V. amygdalina
is a female-dominated enterprise, with participation by men only in the market
gardens (or on the farm).
Part 4: Production Schedules
Some V. amygdalina grown from seeds can thrive well in slightly acidic soil with
low organic matter and high water holding capacity. Water is the key factor for the
growth of its leaves. High yields can be obtained during rainy season (Kayode
2004). V. amygdalina is a short cycle crop which can be harvested twice per month
for up to 7 years. Planting V. amygdalina can be easy because it is compatible with
any type of crop and can be planted in a variety of arrangements (Biggelaar and
Gold 1996).
Part 5: Utilization
Cosmetic
The stem and root divested of the bark are used as chewing-sticks in many West
Africa countries like Cameroon, Ghana, and Nigeria (Yedjou et al. 2008).
Pharmaceutical and Therapeutic
V. amygdalina has been demonstrated to possess active anticancer (Izevbigie 2003;
Agbogidi and Akpomorine 2013), anti-bacteria, antimalaria, and anti-parasitic
(Tadesse et al. 2008) agents. The roots and leaves are used in ethnomedicine to treat
fever, hiccups, kidney problems, stomach discomfort and leukemia (Burkill 1985;
Hamowia and Saffaf 1994; Khalafalla et al. 2009). Pharmacological studies have
also shown that the leaf extracts have both hypoglycaemic and hypolipidaemic
properties in experimental animals and so could be used in managing diabetes
mellitus (Akah and Okafor 1992; Agbogidi and Akpomorine 2013).
Polyherbal preparations with bitter leaf as the active ingredients strengthen the
immune system through many cytokines and chemokines regulation (Agbogidi and
Akpomorine 2013). The herb not only lowers the body sugar level sufficiently, it
also plays a role in the repair of pancreas (Agbogidi and Akpomorine 2013). The
beneficial use of V. amygdalina in animal nutrition in Nigeria has also been well
documented (Onwuka et al. 1989; Aregheore et al. 1998).
Adding bitter leaf to your health diet may reduce the risk of chronic diseases like
breast cancer and type-2 diabetes. Bitter leaf can reduce LDL and total cholesterol.
It is an abundant source of antioxidant agents (Agbogidi and Akpomorine 2013).
Food and Flavoring
Bitter leaf is a traditional ingredient in African cuisine (Agbogidi and Akpomorine
2013). The leaves are one of the most widely leafy vegetables consumed by
Nigerians, and even Cameroonians during special occasions such as marriages, bap-
tisms, Christmas, and birthdays. It is an abundant source of the poly unsaturated
fatty acids, linoleic and linolenic acids which have been found to be protective
against cardiovascular disease (Agbogidi and Akpomorine 2013). But, the dietary
use of the leaves of V. amygdalina has remained limited only to the cultures that use
it as vegetable (after maceration to remove the bitter principles) in soup and porridge
preparation, suggesting an under-utilization of the plants leaves (Ijeh and Ejike 2011).
Industrial
Bitter leaf can be put into use in soap making, ornaments, substituted for hops in
beer brewing, restoration of stamina as well as contribution to carbon sequestration
thereby reducing environmental degradation (Agbogidi and Akpomorine 2013).
The aqueous leaf extract of V. amygdalina has been shown to be phytotoxic. Powders
of dried leaves of V. amygdalina have been reported to have insecticidal potency
against the larvae of Calloso bruchus maculatus and Sitophilus zeamais – insects
responsible for heavy losses of stored cow pea and maize, respectively (Ijeh and
Ejike 2011). It was also reported that the essential oil of V. amygdalina was effective
in the control of Sitophilus zeamais (Ijeh and Ejike 2011).
Others
Unlike other plants that are native to certain areas, it can adapt to a variety of cli-
mates. It is grown in many countries, in savannah zones and cultivated fields
(Agbogidi and Akpomorine 2013).
Safety Data
In an acute toxicity experiment, V. amygdalina was reported to be non-toxic (Ijeh
and Ejike 2011). However, other earlier reports implicate the plant to be toxic in
high doses (Ijeh and Akomas 2005; Ojiako and Nwanjo 2006). This could be
inferred because of the presence of HCN above 4.0 mg/kg in the leaf, besides the
appreciable saponin contents especially if not properly washed (Dike 2010).
Cyperaceae
Cyperus esculentus var. Sativa
Cyperus esculentus is a plant cultivated mainly for its food purpose. But it became
subsequently known for its medicinal value and could safely be referred to a medici-
nal food plant. It thrives in moist sandy-loam soil though, largely insensitive to soil
types (Bamishaiye and Bamishaiye 2011). It is propagated from the tubers or rhi-
zomes, and cultivated anytime within the rainy season. It matures between 90 and
120 days with no requirement for manure or weeding except if necessary (FAO
1988). Being another women-dominated farming, harvest is manually carried out
and labor intensive. It is sun-dried and stored unwashed after harvest. Used widely
in cosmetics, it has been reported to prevent fibrosis, prostate and hernia cyst. It also
has LDL-cholesterol lowering and HDL-cholesterol elevating properties.
Cyperus esculentus, Tiger nuts and plant

Synonyms
Cyperus esculentus var. esculentus, Cyperus esculentus var. hermannii, Cyperus
esculentus var. leptostachyus and Cyperus esculentus var. macrostachyus (Oloyede
et al. 2014).
Common Names
In Nigeria, it is called “Aya” (in Hausa), “imumu” (in Yoruba), “ofio” and “aki
Hausa” (in Ibo), όpu-όpa (Igala); and elsewhere, Zulu nut, yellow nut grass, ground
almond, chufa, edible rush, rush nut, yellow nut sedge or earth almond.
Tiger nut is a tough erect fibrous-rooted perennial plant, 1–3 ft. high, reproducing
by seeds and by many deep, slender rhizomes, which form weak runners above the
ground, and small tubers or nutlets at the tips of underground stems (Bamishaiye
and Bamishaiye 2011). The leaves are long, narrow, shiny, light-green and arranged
in three rows around the triangular stem. They are characterised by pointed tips
separated from the rest of the leaves by a distinct shoulder. The flowers are yellow-
ish or yellowish-brown with spikes which are usually about 1.0–1.5 cm long. The
roots are in form of rhizomes which are yellow in colour and have single tubers seen
as root swellings at the end. These tubers (or root swellings) are generally round,
small and 5.0–20.0 mm in length, with a thin pale brown outer coat which gets
darker with maturity. The tuber is a nicely flavoured sweet nut and crispy (FAO
1988).
Classification
Tiger nut (Cyperus esculentus var. sativa) is one members of the family Cyperaceae
(Bamishaiye and Bamishaiye 2011).
It is widely distributed in the temperature zones within South Europe as its probable
origin, and has become naturalized in Ghana, Nigeria and Sierra Leone (Anon
1992).
Soil Requirements
Tiger nut is found inhabiting wet marshes and edges of streams and ponds where it
grows in coarse tufts. According to Bamishaiye and Bamishaiye (2011), the plant
grows best in moist sandy-loam soils but will grow even in the hardest clay and
tolerates high soil moisture and is intolerant to shade.
Production Levels
It was observed that tiger nut production is predominantly the work of women farm-
ers, as high as 70% (Bamishaiye and Bamishaiye 2011; Tetteh and Ofori 1998).
Ghana, Nigeria and Sierra Leone (Anon 1992).
Cultivars
There are mainly three varieties namely: black or dark brown (‘Fanti’), brown and
yellow (‘Kwahu’) (Tetteh and Ofori 1998; Okafor et al. 2003), and only yellow and
brown are readily available in the Nigerian markets (Okafor et al. 2003). The yellow
variety is preferred to all other varieties because of its inherent properties like its
bigger fleshier size, and attractive colour. The yellow variety also yields more milk,
contains lower fat, higher protein and less anti-nutritional factors especially poly-
phenols (Okafor et al. 2003).
Propagation
It is propagated from the tubers and their rhizomes (Bamishaiye and Bamishaiye
2011).
Soil Preparation
Experiments revealed that tuber production in tiger nut does not appear to be sensi-
tive to soil type, but nutrient additions can increase tuber production. The increase
in production is most likely tied to above ground vegetative biomass such that the
larger the plant, the more tubers are produced. Therefore, from a production per-
spective, management techniques that enhance growing conditions are likely to be
beneficial. Similarly, early sowing leads to a longer growing season which is cor-
related with higher production. Lastly, early emergence increases production
because competing vegetation will not be as well established and will therefore have
less impact on growing conditions (Dyer 2009).
Planting
It is usually sown in April and picked in November (Osagie et al. 1986). Before
planting, the nuts are soaked in water for 3–4 days to facilitate germination. The
water is changed every day to prevent rot. The soaked nuts may be planted directly,
or kept in a basket until they begin to sprout between 7 and 12 days after soaking.
The sprouted nuts are picked daily and planted. Pre-sprouting before planting
ensures uniform emergence and a perfect stand. Although, tiger nut grows well
when planted as a sole crop, most farmers in the study areas usually intercropped
them with maize, cassava or cocoyam (Tetteh and Ofori 1998).
Fertilization
Fertilizer does not have a consistent effect on tiger nut tuber production (Dyer
2009), as the work of Tetteh and Ofori (1998) revealed that no form of manure
(organic) or inorganic fertilizer needs to be applied to tiger nut. Most producers
claim that when any form of manure is applied, the resulting nuts are less tasty and
less attractive (Tetteh and Ofori 1998).
Irrigation
While in some parts with moderate rainfall, tiger nuts can thrive and produce good
yields, in most parts, it requires copious amount of water and repeated watering
especially during the dry season (FAO 1988).
Weed Control
According to Tetteh and Ofori (1998), weeding is done less often and mostly only
once during the life of the crop. Weeding has been found to be unnecessary when
pre-sprouted nuts were sown on newly made mounds, as they sprout early and grow
faster to suppress weeds that germinate afterwards. Weeding becomes necessary
only when inter-mound spacing is too wide. The weeds are hand-picked to avoid
disturbing the developing nuts.
Pest Control
Termites and grass cutters have been reported as some challenges to tiger nuts. In
order to reduce losses, harvest is done as soon as nuts mature (Tetteh and Ofori
1998). To avoid termites attack, plots where termite mounds are sited must be
avoided.
Disease Control
Usually there are no common diseases that seriously affect tiger nuts. However,
Negro bug known as Corimelaena pulicaria punctures the tubers while root knot
nematodes (Meloidogyne spp.) also cause damages to the tubers before they mature
(FAO 1988).
Harvesting
Signs of maturity include yellowing of leaves and cessation of new inflorescence.
The nuts are harvested by hand-picking singly from the soil, a slow and labour –
intensive process (Tetteh and Ofori 1998). However, most farmers harvest the nuts
by gradually loosening the soil around the plants to expose them, and the plants are
raised and the attached nuts are removed by hand. But the nuts left in the soil are
slowly and tediously hand-picked singly (Tetteh and Ofori 1998).

The tubers are the produce of interest in the tiger nut plant. These are considered to
have reached maturity when the plants begin to wilt, and are found within the top
15–20 cm of the soil. They are harvested by hand-picking (Tetteh and Ofori 1998)
or by running a small turning plough beneath them. Groundnut diggers have also
been used. The tubers are then separated, soil removed, washed and allowed to
dry for between 1 and 3 days before grading and storage in thin layers in sheds
(FAO 1998).
Grading
They are generally dried out (mostly in the sun), a process that takes one or more
months with occasional turning over to ensure uniform drying to preserve them and
to eliminate prevailing rot or any microbial infection (Bamishaiye and Bamishaiye
2011).
Packaging/Storage
Usually, they are not washed after harvest, but are sun dried thoroughly and packed
in jute bags or specially woven baskets called kentenku for up to 12 months (Tetteh
and Ofori 1998). No preservatives are applied. Tiger nut can be processed into
flower via drying under the sun or roasting to dehydrate the product. This is then
introduced to a hopper for milling. The product is allowed to cool then packaged in
air-tight containers and stored (Bamishaiye and Bamishaiye 2011). When processed
into tiger nut oil, the purified cold extracted oil is collected in barrels and stored
Marketing
Tiger nuts are readily available in the Nigerian markets. In Northern Nigeria, the
nuts can be bought in the market all year round (Bamishaiye and Bamishaiye 2011).
According to Tetteh and Ofori (1998), units of sale include 50 kg minibags, ‘size
34’ bucket, 3 kg measure, and 450 g cans. Usually, farmers sell their nuts to middle-
men who in turn sell to retailers in the urban centres. Most farmers sell their produce
immediately after harvest because of lack of appropriate storage facilities, or the
need for quick money (Tetteh and Ofori 1998).
Tiger nuts usually take between 90 and 120 days to mature. On sandy soils the aver-
age yields are between 789 and 900 kg/ha. In large scale production aided by irriga-
tion, yields could be as much as 8–14,000 kg/ha. The seed rates are reported to be
between 17 and 45 kg/ha, with a spacing of 15 × 75 – 90 cm which requires 35–45
kg/ha of tubers (FAO 1988).
Part 5: Utilization
Cosmetic
The oil is high oleic acid and low polyunsaturated fatty acid (linoleic acid and lino-
lenic acid), and is suitable for salad making and for the skin. It also has higher oxi-
dative stability than other oils, due to the presence of polyunsaturated fatty acids
and γ-tocoferol.
Tiger nuts have been considered to have adequate properties to fight respiratory
infections, stomach aches, promotes production of urine. Hence, it is considered
effective in the prevention of cyst, prostrate, hernia, rectum deformation and pro-
lapse, endometriosis or fibrosis as well as fallopian tube blockage. The oil reduces
low density lipoprotein-cholesterol (LDL-C) and increases high density lipoprotein-
cholesterol (HDL-C), reduces level of triglycerides in blood and the risk of forming
bloody clots, thereby preventing arteriosclerosis. It is also reported to stimulate the
absorption of calcium in bones and production of new bony material, and is recom-
mended for infants and the elderly because of its high vitamin E, and hence its
antioxidant benefits in the cell membrane.
Food and Flavoring
Tiger nut has been cultivated as a livestock food and for human consumption. It can
be eaten raw, roasted, grated, baked or used for ice cream and beverage making for
its many useful benefits (Bamishaiye and Bamishaiye 2011). Tiger nut milk is a
delicious and energizing drink that is usually served chilled, as it is, or packaged
Industrial
Tiger nut flour has a unique sweet taste, which is a good alternative to many other
flours like wheat flour, used in the confectionery industry as it is gluten free and
good for people who are averse to gluten in their diets (Belewu and Abodunrin
2006; FAO/WHO 1985). It is considered good flour or additive for the bakery indus-
try (Anderson et al. 1994) as flavoring agent for ice creams and biscuits (Osagie and
Eka 1998). In the textile industry, the oil is used to water proof fibres (Bamishaiye
and Bamishaiye 2011).
Safety Data
The presence of anti-nutrients like tannins have been reported (Bamishaiye and
Bamishaiye 2011), and may be responsible for the toxicity of the hexane extract in
brine shrimp lethality assay (Tetteh and Ofori 1998). While these anti-nutrients can
be eliminated by boiling in water (Bamishaiye and Bamishaiye 2011), the methano-
lic extract, is relatively non-toxic (Tetteh and Ofori 1998).
Gramineae (Poaceae)
Cymbopogon citratus Stapf. Cymbopogon citratus Stapf is a plant cultivated mainly

around homes. It performs well on sandy or clay-loam soils with good drainage.
Being a plant considered on poor soils, application of fertilizer is usually a rare
consideration. With adequate rainfall, irrigation is not required (Directorate 2012).
Several diseases of C. citratus are mostly fungal. Thus, the application of appropri-
ate fungicide before flowering in all cases is necessary. Harvest is done mechani-
cally or manually. C. citratus possess anti-carcinogenic, anti-inflammatory,
antioxidant, and cardioprotective properties. The issue of the toxicity or not of the
plant is still in debate. However, C. citratus has been reported to be a good source
of antioxidants such as flavonoids, and therefore, its tea remains a nutritionally

acceptable and medicinally valuable beverage (Ekpenyong et al. 2014).
Cymbopogon citratus (Lemon grass)
Description of Plant
Lemon grass is a tufted perennial grass growing to a height of 1 m with numerous
stiff leafy stems arising from short rhizomatous roots (Karkala and Bhushan 2014).
It has an economic lifespan of about 5 years (Carianne 2005). The leaf-blade is
linear, tapered at both ends and grows to a length of 50 cm and width of 1.5 cm.
The leaf-sheath is tubular in shape and acts as a pseudo-stem. The leaves are long,
glaucous, green, linear, tapering upwards and along the margins. C. citratus plant
produces flowers at matured stages of growth (Directorate 2012; Karkala and
Bhushan 2014).
Classification
Cymbopogon citratus Stapf is popularly known as citronella grass or lemon grass.
This specie belongs to the Gramineae (Poaceae) family (Soares et al. 2013; Karkala
and Bhushan 2014).
Lemon grass is widely cultivated in the tropics and subtropics (Directorate 2012).
Soil Requirements
Lemon grass is widely adapted to a range of soils and performs well on sandy to
clay loam soils with a pH range of 5.0–8.4 and good drainage. The lower the altitude
and more alkaline the soil, the higher is the citral content of the oil. The variety with
high citrates is in demand. Drier and loamier soils yield higher citral content. Lemon
grass can be an option for consideration in poor soils, alkaline soils, steep slopes,
and degraded forests and rehabilitation of non-forest mining and industrial waste
lands (Directorate 2012).
Production Levels
The yield of oil is less during the first year of establishment and increases in the
second year and reaches a maximum in the third and fourth years, after which it
declines. For economy, the plantation is maintained only for 6 years. On average,
30–50 metric tons of fresh herbage is harvested per hectare per annum, which will
yield 100–250 kg of oil at an oil yield ranging between 0.2 and 0.5%. Under irri-
gated conditions and better management with increased herbage, an oil yield of up
to 500 kg/ha can be achieved (Directorate 2012).
Lemongrass is grown throughout Africa, in the Democratic Republic of the Congo
(DRC), Angola, Madagascar and Comoros islands although, Guatemala is known to
be the leading exporter of C. citratus products (Directorate 2012).
Cultivars
In Southern Africa for instance, there are no registered cultivars for lemongrass.
But there are two known types of C. flexuosus. The red-stemmed grass, which is
known as the true lemon grass and is recommended for higher oil yields, and the
white-stemmed (C. flexuosus var. albiscens) yields oil of lower citral content
(Directorate 2012).
Propagation
Although propagation can be achieved by the seeds, but for better quality and yield
of oil, growing lemon grass by the slips obtained from divided, well-grown clumps,
is recommended. Tops of clumps are cut off within 20–25 cm of the root and the
latter divided into slip and the lower brown sheath removed to expose young roots
(Directorate 2012).
Soil Preparation
In order to obtain product yields of high quality that is in demand globally, C. citra-
tus and essential oil crops are to be grown on natural soils (Directorate 2012;
Ekpenyong et al. 2014). Thus, soil fertility, pH, balanced mineral status are to be
ascertained.
Planting
Directorate (2012) recommended a row spacing of 20 cm with a row width of 40 cm,
that will give a total of 125,000 plants per ha in a high rainfall area or under irrigation.
In areas with lower rainfall, 60,000 plants per ha is an advisable density. An initial
high planting rate can be used and as the plants mature, every second plant can be
taken out and divided again for new slips. The space created in this way can then be
filled with remaining plants as they mature (Directorate 2012).
Fertilization
Application of fertilizer should be dependent on soil analysis (Directorate 2012),
which should indicate any nutrient, compound or element that is lacking and needs
to be replenished.
Irrigation
Lemon grass has a high water requirement. Where annual rainfall exceeds 650 mm,
irrigation is not necessary. C. citratus is more drought tolerant than C. flexuosus.
Overhead, flood and drip irrigation can be used. Where rust is a problem, overhead
irrigation might not be a good option (Directorate 2012).
Weed Control
Hand-weeding and hoeing are very important as weeds affect the yield and quality
of the oil (Directorate 2012). About 2–3 weedings are required in a year. Distillation
waste of this crop applied as organic mulch at 3000 kg/ha is highly effective for
controlling weeds on C. citratus farm. Another very good method of controlling
weed is by excluding the sunlight through planting in such a way that the plant
should form canopy quickly (Directorate 2012).
Pest Control
Various pests like stem-boring caterpillar and nematodes can result to enormous
damage and reduction in quality and yield. In the former, burning of the dry stubble
during the off-season in summer would destroy the caterpillars lurking inside the
stubble but if mild, the affected shoots are pulled out and destroyed. In severe
attacks, chemical control is needed (Directorate 2012). In the later situation, soil
solarisation is effective but as a last resort. Due to toxicity, nematicide becomes
necessary (Directorate 2012) however, with caution.
Disease Control
Recognizing the first symptoms of disease is quickly followed with the removal of
diseased plants and continuous monitoring of fields for signs of pathogen recur-
rence or spread of disease is very helpful. Some major diseases of C. citratus are
Long smut, Red leaf spot, Leaf blight, and Rust. Spraying with a recommended
fungicide before flowering in all cases is necessary (Directorate 2012).
Harvesting
First harvest of the plant can be done within the first 6–9 months of planting the
slips then on a monthly basis afterwards (Directorate 2012; Ekpenyong et al. 2014)
as the plant actively grows. Frequent cutting actually stimulates vegetative growth.
Moreover, oil yield is reduced if the plant is allowed to grow very large. Early in the
morning harvest is recommended, which is done mechanically or by hand
(Directorate 2012). Cutting too low and splitting of cutting edges should be avoided.
Three harvests can be obtained in the first year and up to 5–10 harvests during each
of the 3–5 succeeding years, depending on soil moisture status, management and
weather (Directorate 2012).

C. citratus leaf, stem, and roots are commonly used in herbal medicine (Ekpenyong
et al. 2014).
Grading
The quality of lemon grass oil is determined by the content of citral, a terpene alde-
hyde, which is mainly used for the production of vitamin A (Directorate 2012).
Packaging
Essential oils are volatile and as such have to be handled with care. Lemon grass
should be packed firmly as this prevents the formation of steam channels. If the
grass is too long it can be cut into smaller lengths to ensure firm packaging
(Directorate 2012).
Storage
Lemon grass oil should be stored in dark, air-tight, glass bottles and away from heat
or heavy metals. Once opened, refrigeration and tight sealing of the cap helps pro-
long its shelf-life. Deterioration begins if the liquid is much darker or more viscous
than normal. Lemon grass oil is very acidic and will destroy plastic and rubber
within a relatively short time (Directorate 2012).
Marketing
C. citratus oil price fluctuates on the international market due to the interplay of
demand and supply that can vary widely (Directorate 2012). As such, price is depen-
dent both on the extent of availability of the oil and exchange rate at the time of sale.
Important also, is the quality of the available oil on the global market (Directorate
2012).
While the citral content (Halabi and Sheikh 2014) which is a mixture of terpenoids,
neral and geranial confers the lemony smell actually justifies the large scale com-
mercial cultivation of C. citratus, agro-climatic factors like soil water, and soil
salinity influences the growth, quality and yield of the oil which is the most impor-
tant product from C. citratus (Ekpenyong et al. 2014). It has been observed that a
better yield of the oil and higher citral content is obtainable from C. citratus har-
vested during the drought season (March–June) than any part of the year (Ekpenyong
et al. 2014). Also, the early or delayed harvest of C. citratus affects essential oils
and citral contents of the plant, with significantly higher oil content found in plants
harvested at 6 ± 0.5 months of the plant’s growth than those harvested much later
(Ekpenyong et al. 2014).
Part 5: Utilization
Cosmetics
In cosmetics, its essential oils are used as fragrance in the manufacture of perfumes,
soaps, detergents, and creams (Ekpenyong et al. 2014).
It is used in herbal medicine for a wide range of applications based on its anti-
carcinogenic, anti-inflammatory, antioxidant, cardioprotective, antitussive, and
anti-rheumatic activities (Ekpenyong et al. 2014). It has also been used to inhibit
platelet aggregation, treat diabetes, dyslipidemia, gastrointestinal disturbances,
anxiety, as well as in aromatherapy (Ekpenyong et al. 2014).
Food and Flavoring
C. citratus is also consumed as a tea, added to non-alcoholic beverages and baked
food, and used as a flavoring and preservative in confections and cuisines (Ekpenyong
et al. 2014).
Industrial
Although, the down-side in herbal medicine use is in the lack of dosage of adminis-
tration as in conventional drugs, it has been reported that a team of researchers have
produced tablets of C. citratus containing powdered dry C. citratus leaves using
acacia and gelatin as binders at concentrations of 2, 4 and 8% w/w (Ekpenyong
et al. 2014).
Safety Data
A number of studies have attempted to answer the safety question with conflicting
and inconclusive data. Thus, while some studies observed toxic effects, no toxicity
was detected by others. However, most of these studies were performed in animals,
making the results difficult to apply to humans (Ekpenyong et al. 2014). However,
C. citratus has been used over many years to make caffeine-free tea and as an herbal
drink, suggesting that it may be a healthier alternative to caffeine-containing tea
products (Blanco et al. 2009). Akande et al. (2011) found that, in comparison to
other tea brands consumed among Nigerians, C. citratus tea was a good source of
antioxidants such as flavonoids and therefore, a nutritionally acceptable and medici-
nally valuable beverage (Ekpenyong et al. 2014).
Meliaceae
Khaya senegalensis(Desv.) A. Juss
Khaya senegalensis (Desv.) A. Juss is propagated by seeds, preferably from nursery

and grows well on a wide range of soil conditions. Fertilizer application during
planting is recommended. Survival rates are low so, regular watering of seedlings of
Khaya senegalensis is necessary, besides weed control which is manually done by
hoeing (Nikiema and Pasternak 2008). The leaves are used as remedy for diseases
like diarrhea, gynecological disturbances, digestive and nervous disorders. The
stem-bark and leaves have been reported to be used to cure syphilis, pyrexia, and
malaria fever (Kubmarawa et al. 2008). However, safety reports have indicated that
extracts may affect cellular integrity of vital organs of the body.
Khaya senegalensis (http://nurserylive.com/images/)
Botanical Name
Khaya senegalensis(Desv.) A.Juss (Common and local names: Mahogany (English).
In Nigeria, it is called ‘Madachi’ in Hausa, ‘Ono’ in Igbo, ‘Oganwo’ in Yoruba,
‘Ago’ in Igala, ‘Wuchi’ in Nupe, (Mann et al. 2003) and Dalehi-kahi in Fulani.
Khaya senegalensis is an evergreen tree with shinny foliage, pinnate leaves and
characteristic round capsules. It can grow to a height of 30 m and a girth of 3 m with
dense crown and short bole covered with dark grey scaly bark, with slash dark pink
and bitter yielding gum when wounded. The leaves have 3–4 (exceptionally 5) pairs
of leaflets, 5–10 cm long by 2.5–5 cm broad, more or less elliptic, round, obtuse or
shortly acuminate at apex stalks of leaflets 4 mm long (Kubmarawa et al. 2008).
The fruit is an upright, almost spherical woody capsule, 4–6 cm in diameter, opening
by four valves from the apex. The seeds are brown, six or more per cell, broadly
transverse ellipsoid to flat, about 25 × 18 mm, margins and narrowly winged (Orwa
et al. 2009).
Classification
Khaya senegalensis belongs to the Meliaceae family (Orwa et al. 2009).
Khaya senegalensis occurs from Mauritania and Senegal east to Northern Uganda.
It is found in many African countries like Cape Verde, Tanzania, Malawi,
Madagascar, Egypt and South Africa. It is also found in India, Indonesia, Vietnam,
Australia Sri Lanka and tropical America. It is commonly planted as ornamental and
roadside tree (Nikiema and Pasternak 2008).
Soil Requirements
Khaya senegalensis prefers deep and well-drained alluvial soils and termite mounds,
but can also be found on shallow, rocky soils, where it is usually much smaller. It
tolerates flooding in the rainy season (Nikiema and Pasternak 2008). Khaya senega-
lensis thrives on a wide range of soil conditions, from neutral to strongly acidic, and
from very well-drained coarse sandy loam to somewhat poorly drained clay (Orwa
et al. 2009). It occurs in savanna woodland, often in moist localities and along water
courses in areas with 650–1300 (~1800) mm annual rainfall and a dry season of 4–7
months (Nikiema and Pasternak 2008). It thrives in areas with mean annual tem-
perature of between 24.5 °C and 31.5 °C and mean annual rainfall 400 mm to
1750 mm (Orwa et al. 2009).
Production Levels
In experimental plantations in Burkina Faso the annual production of Khaya sene-
galensis is estimated at 3.7 m3/ha (Nikiema and Pasternak 2008).
Cameroon, Central African Republic, Chad, Cote d’Ivoire, Equatorial Guinea,
Gambia, Ghana, Guinea, Guinea-Bissau, Mali, Niger, Nigeria, Senegal, Sierra
Leone, Sudan, Togo and Uganda (Orwa et al. 2009).
Propagation
Khaya senegalensis is propagated by seeds, preferably from nursery Wildings are
sometimes collected for planting. It also reproduces by root suckers, while grafting
and layering are also possible. However, propagation by cutting is much more dif-
ficult (Nikiema and Pasternak 2008).
Soil Preparation
Khaya senegalensis seeds are best raised in seed beds in the nursery or in pots
(Nikiema and Pasternak 2008).
Planting
High quality Khaya senegalensis seeds are selected for planting, and soaking of
seeds for 24 h in water improves germination. The seeds can best be sown in seed
beds in the nursery or in pots. Fresh healthy seeds have a high germination rate of
90–100%, and they may retain their viability for 6–8 months. Upon sowing, seeds
are covered with only a thin layer of soil, or left partially uncovered. Germination
takes 10–18 days (Nikiema and Pasternak 2008).
Fertilization
The application of a complete fertilizer at a rate of 200 g/tree at the time of planting
is recommended (Nikiema and Pasternak 2008).
Irrigation
For good survival rates, regular watering of Khaya senegalensis seedling is essen-
tial. It also occurs along water courses (Nikiema and Pasternak 2008).
Weed Control
The recommended method of weed control on Khaya senegalensis plantation is by
hoeing especially at the onset of the dry season (Orwa et al. 2009).
Pest Control
Khaya senegalensis is susceptible, in its natural range to severe attack by shoot bor-
ers (Hypsipila robusta) which may result in mis-shaped trees with no timber value.
The sapwood is susceptible to attack by long-horn beetles and powder post beetles
(Lyctus spp.). A bacterial disease of dry-zone mahogany in the Sudan caused by
Xanthomanus khaye results in rough, scabby leaf spots and knobby stem cankers
(Orwa et al. 2009).
Harvesting
Logs are harvested from Khaya senegalensis by felling using traditional equipment,
which can be quite difficult because of the dense and fairly hard wood. The bark is
collected whenever needed and many large trees show signs of debarking. The
leaves are also harvested for fodder (Nikiema and Pasternak 2008).

The logs are collected for timber by felling and the bark and leaves are also col-
lected for traditional medicine and fodder respectively. Traditional methods of col-
lection are used (Kubmarawa et al. 2008).
Marketing
Khaya senegalensis is widely used on commercial scale, particularly in West Africa
as timber (Nikiema and Pasternak 2008).
Khaya senegalensis is grown from the seeds which are dispersed as far as 100 m by
wind. The seeds bearing fruits are developed at about 20–25 years of the tree’s exis-
tence, and cultivation is successful in areas with short dry seasons and high rainfall
(Orwa et al. 2009). Wijenayake (2014) however, reported that it is a fast growing
tree which can reach a height of 30–33 meters and maturing in 10–12 years, and
new plants could be produced by tissue culture.
Part 5: Utilization
Cosmetic
The seed oil is used in cosmetics production (Nikiema and Pasternak 2008).
Pharmaceutical and Therapeutics
Khaya senegalensis is used as a remedy for diseases such as diarrhea, gynecological
disturbances, digestive disorders and nervous confusions (Kubmarawa et al. 2008).
Decoction and concoction of the stem-bark and leaves are used to cure mucous diar-
rhea, syphilis, pyrexia, and malaria fever. Dried stem-bark is used externally for the
treatment of skin infections and blennorrhagra, while the fixed oil is used to treat
leprosy, syphilis, dermatomes, sores and ulcer in adults. The flowers are used to
treat stomach diseases and as an ingredient in anti-syphilic prescriptions. The oil
from the seeds is used as emmenegogue (Kubmarawa et al. 2008). Bark decoctions
or macerations are used to treat malaria fever, stomach complaints, diarrhea, dysen-
tery and anaemia, as anodyne in cases of rheumatism, headache, and as tonic and
anthelmintic. They are also used as purgative, antidote to treat syphilis, leprosy,
chickenpox and angina. The bark is applied externally as disinfectant in cases of
inflammations and skin diseases, rash, scabies, wounds, ulcers, boils, haemorrhoids,
swellings and toothache. For veterinary purposes, the bark is also used as anthel-
mintic, tonic, appetizer, and to treat trypanosomiasis, liver flukes, diarrhea and
ulcers (Nikiema and Pasternak 2008).
Food and Flavouring
The foliage is common source of fodder, though of low fodder quality which is
commonly used towards the end of the dry season when better quality fodder is not
available, or in mixtures with better fodders. The seed oil is used for cooking
(Nikiema and Pasternak 2008).
Industrial
Khaya senegalensis wood is widely used for carpentry, joinery, furniture, cabinet
work, ship building, decorative veneer and implement handle (Nikiema and
Pasternak 2008; Orwa et al. 2009).
Others
The wood ash of Khaya senegalensis is added to stored grain to prevent infestation
by insects. The tree is commonly planted as a roadside tree, ornamental shade tree,
and sometimes for soil stabilization (Nikiema and Pasternak 2008).
Safety Data
A study of the sub-chronic effect of aqueous stem bark extract of Khaya senegalen-
sis on some biochemical, haematological and histopathological parameters of rats
showed that the extract may affect cellular integrity of vital organs of the body (Onu
et al. 2013).
Aqueous and ethanolic extracts of root, stem-bark and leaves of Khaya senegalensis
contain saponins, tannins and phenols (Kubmarawa et al. 2008). Limonoids have
been identified in Khaya senegalensis stem, bark, leaves and flowers: khayanolides
D and E, khayanolides, 2,6-dihydrolissinolide. Two mexicolides named khayanone
and 2-hydroxyseneganolide have also been identified. Three other mexicanolide
limonoids named seneganolide A, 2-hydroxyseneganolide A and
2-acetoxyseneganolide A (Kubmarawa et al. 2008). Simlarly, Atawodi et al. (2009a,
b) have reported the presence of catechin, rutin and quercetin rhamnoside in the
leaf, and catechin and procyanidins in the stem bark. Saponins, flavonoids, tannins,
alkaloids, glycosides and carbohydrates were detected in the ethanolic leaf extracts
(Wakirwa et al. 2013).
The aqueous extract of Khaya senegalensis stem-bark and the ethanolic extract of
the root, stem-bark and leaves, using disc diffusion method exhibited antibacterial
activity against Staphylococcus aureus, Streptococcus spp., Eschrichia coli,
Pseudomonas aeruginosa, Salmonella spp. and Bacillus subtilis (Kubmarawa et al.
2008). A comparison of the antibacterial activity of the aqueous, ethanolic and ace-
tone extracts of the bark against Staphylococcus aureus, Streptococcus pyorogenes,
Salmonella typhi, Shigella dysenteriae, Klebsiella pneumonia and Pseudomonas
aeruginosa showed that the acetone extracts had higher antibacterial activity. The
minimum inhibitory concentration of the acetone extract was in the range of 6.5–
12.5 mg/ml while the minimum bactericidal concentration ranged between 6.5 and
25.0 mg/ml. The antibacterial activity was not significantly affected by heating at
100 °C for 1 h but the activity was lost at pH 8.0 (Sale et al. 2008). The ethanolic
leaf extract of Khaya senegalensis was examined for antimicrobial activity against
some selected clinical isolates: Staphylococcus aureus, Escherichia coli, Klebsiella
pneumonia and Candida albicans. Dose dependent activity was observed with
zones of inhibition ranging from 15.00 to 26.00 mm for S. aureus, 10.00–23.50 mm
for E. coli and 3.20–16.00 mm for K. pneumonia. There was no inhibition against
the growth of C. albicans. Minimum inhibitory concentrations for the clinical iso-
lates are 25.00 mg/ml, 12.50 mg/ml, 12.5 mg/ml and no effect respectively, while
the minimum bactericidal concentrations were 50.00 mg/ml and 25.00 mg/ml for S
aureus and E. coli respectively (Wakirwa et al. 2013). Earlier on, the antioxidant
effect of this plant has been described (Atawodi et al. 2009a, b).
Moringaceae
Moringa oleifera Lam.
Moringa oleifera (http://kingraah.blogspot.com.ng)
Moringa oleifera Lam. is a smallish deciduous tree which possesses relatively
scanty foliation. It looks more like leguminous plant. It grows up to 8 m high, and
bearing branches that are often forked from near the base. The bark could be smooth,
dark grey, slash thin or yellowish. Twigs and shoots are short and densely hairy.
The crown is wide, open, typically umbrella shaped and usually a single stemmed
plant which is often deep rooted. The wood is soft.
Leaf arrangement is alternate, the old ones get yellowish and falls off. These
leaves are large (up to about 90 cm long), with opposite pinnae, spaced about 5 cm
apart up the central stalk, usually with a 2nd lot of pinnae, also opposite, bearing
leaflets in opposite pairs, with a slightly larger terminal leaflet. Leaflets are dark
green above and pale on the under surface and are of various sizes and shape,
although usually rounded-elliptic, scarcely as much as 2.5 cm long.
Flowers are produced throughout the year, in loose axillary panicles up to 15 cm
long; individual flower stalks up to 12 mm long and very slender; 5 pale green
sepals 12 mm long, finely hairy, 5 white petals, unequal, a little longer than the
sepals; 5 stamens with anthers, 5 without; style slender, and the flowers sweet
smelling.
Fruits are large and distinctive, between 30 to 90 cm long and 12 mm broad,
slightly constricted at intervals, gradually tapering to a point, 3-(4-) angled, with 2
grooves on each face, and light brown in color. It splits along each angle to expose
the rows of rounded blackish oily seeds, each with 3 papery wings (Orwa et al.
2009; Paliwal et al. 2011).
Classification
Moringa oleifera Lam. belongs to the family of Moringaceae (Paliwal et al. 2011).
Moringa oleifera Lam. is indigenous to south Asia and has been found to grow in
Pakistan, West Bengal and India. It has also been introduced to many countries,
including Afghanistan, Bangladesh, Sri Lanka, Southeast Asia, West Asia, Arabian
Peninsula, East and West Africa, Southern Florida, West Indies, Mexico, Peru,
Paraguay and Brazil (Paliwal et al. 2011).
Soil Requirements
Moringa oleifera Lam. can grow at elevations of up to 1400 m along the rivers of its
native range, on sandy or gravely alluvial soils. The soils are usually well drained
and low in organic matter. Although, it can thrive in drought environment for several
months, the water table should be located within the maximum rooting depth of the
plant. Away from its natural origin, it grows well from sea levels up to a 1200 m
elevation in most light to medium textured soils. However, it best grows on sandy
loam soils (Parrotta 1993).
Production Levels
Under ideal conditions, Moringa oleifera can grow very rapidly from seed. Within
the same year, they can produce vegetable pods for market. For instance, early flow-
ering types can produce market-mature pods in 6 months compared to over 1 year
for other types. After coppicing, there is rapid growth and immature pods could be
harvested in half a year (Paliwal et al. 2011).
Moringa oleifera has been reported to be grown in Nigeria, Egypt, Sudan, and vari-
ous parts of West, East and South Africa (Stevens et al. 2013; Baba et al. 2015).
Cultivars
Through genetic manipulations, many different cultivars have been reported. Some
of the cultivars obtainable from different countries include TOT4100 (Taiwan),
TOT4880 (USA), TOT4977 AVRDC, TOT5077 AVRDC, TOT5028 AVRDC,
TOT5169 AVRDC, TOT4893 AVRDC, TOT5330 AVRDC, TOT7266 AVRDC and
TOT4951 AVRDC (World Vegetable Centre, Thailand), SH Silver Hill CHM Silver
Hill and Limpopo (South Africa) (Ndhlala et al. 2014).
Propagation
Moringa oleifera is propagated through both sexual and asexual (vegetative) means.
That is, either by seeds or cuttings, respectively. Seeds are planted about an inch
below the soil surface and can be sprouted all year round in a well-drained soil.
Propagation by cutting is usually done using coppiced portions from the stem or
more commonly from the branch (about 1–2 m long). It is inserted 1/3 portion under
the soil (Paliwal et al. 2011; Foidl et al. 2016). Sprouting which occur between 7
and 30 days of planting is commonly between 60 and 90% for fresh seeds. However,
viability drops drastically after about 2 months of collection or in storage. Partial
shadding is favourable to germinating seeds and seedlings. Moringa oleifera raised
from seeds have been reported to be slower at both fruit yield and lower quality
fruits. However, it has been reported that trees propagated from seeds yields longer
roots than those from cuttings (Parrotta 1993).
Soil Preparation
Whether it is seedlings from the nursery, ungerminated seeds or cuttings, the soil
should be loose and well drained, and moist enough to enhance growth. With avail-
ability of water, planting can be done all year round. Planting pit is dug, watered and
then filled with top soil mixed with compost or manure before planting. When cut-
tings are planted in the nursery, the root system develops slowly. As such, addition
of phosphorus to the soil could be done in order to encourage root development
(Zaku et al. 2015).
Planting
When planting from seedlings, they can be out-planted when they are 60–90 cm
high. A hole is cut in the bottom of the sack, big enough to allow the roots to
emerge. In order to enhance rapid germination, the seeds can be pre-treated either
by soaking the seeds in water overnight prior to planting, cracking the shells before
planting, or removing the shells and planting the kernels only (Zaku et al. 2015). For
intensive moringa production, the trees are planted every 3 m in rows, 3 m apart.To
ensure sufficient sunlight and airflow, it is recommended to plant in an east-west
direction. When the trees are part of an alley-cropping system, there should be 10 m
between the rows (Zaku et al. 2015).
Fertilization
Moringa plant grows generally well without fertilizer addition. For extremely poor
soils however, manure or compost can be mixed with the soil used to fill the planting
pits. Phosphorus can be added to encourage root development and nitrogen will
encourage leaf canopy growth. In some countries like India, 15 cm-deep ring
trenches are dug about 10 cm from the plant during the rainy season and filled with
green leaves, manure and ash. This approach has been reported to promote higher
pod yields. Research done in India has also showed that applications of 7.5 kg farm-
yard manure and 0.37 kg ammonium sulfate per tree can increase pod yields by
threefold (Zaku et al. 2015), but in Nigeria, inorganic fertilizer is hardly used,
although a small proportion of growers in northern Nigeria apply manure.
Irrigation
Moringa tree is a drought – resistant plant that requires no need for much watering.
However, during extended and harsh drought conditions, it might be necessary to
water regularly for the first 2 months and afterwards only when the tree is obviously
suffering from dehydration. Moringa trees starts flowering and producing pods
when sufficient water is available. If rainfall is continuous throughout the year, the
trees will have a nearly continuous yield. In arid conditions, flowering can be
induced through irrigation (Zaku et al. 2015).
Weed Control
Spaces between trees must be free of weeds. Moringa tree root system does not
compete with other crops for surface nutrients and the light shade provided by the
tree will be beneficial to those vegetables which are less tolerant to direct sunlight.
From the second year onwards, moringa can be inter-cropped with maize, sunflower
and other field crops. Sunflower is particularly recommended for helping to control
weed growth. However, Moringa trees are reported to be highly competitive with
eggplant (Solanum melongena) and sweet corn (Zea mays) and can reduce their
yields by as much as 50% (Zaku et al. 2015). Therefore, although practiced in
Nigeria and other countries, mixed and inter-cropping with moringa should be done
with caution.
Pest and Disease Control
Although, moringa is resistant to most pests, there are many that affects them
including bark-eating caterpillar, Indarbela quadrinotata Wlk; the hairy caterpillar,
Eupterote mollifera Wlk.; the green caterpillar, Noorda blitealis Wlk.; and the bud-
worm, N. moringae Tams, which can cause serious defoliation, and many others.
Some other insect pests that cause minor or only occasional serious damage have
also been reported. In Puerto Rico, M. oleifera have been reported to succumb to
termites’ attack, while seed predation has also been identified elsewhere. But in
India and sub-saharan african countries like Nigeria, various caterpillars causing
defoliation are successfully being controlled by spraying synthetic and natural
insecticides (Parrotta 1993; Orwa et al. 2009; Zaku et al. 2015).
Harvesting
In Nigeria, the leaf is used as vegetables, and hence harvested when not fully mature,
but when sold for making moringa tea, it is harvested and sun-dried. In other coun-
tries where they are consumed by humans, harvest is done when the pods are still
young and snap easily to prevent pods from developing a tough exterior as they age,
although the white seeds and flesh remain edible until the ripening process begins.
When producing seed for planting or for oil extraction, the pods are allowed to dry
and turn brown on the tree. In some cases, it may be necessary to prop up a branch
that holds many pods to prevent it from breaking off. Harvesting the pods before
they split open and seeds fall to the ground is necessary. Seeds are stored in well-
ventilated sacks in dry and shady places (Zaku et al. 2015) away from rodents and
other storage pests.

It has been said that ‘every part of the moringa tree is said to have beneficial proper-
ties that can serve humanity’ and hence collected, they will best serve the desired
purpose. For example, the seeds are highly useful in making M. oleifera oil and are
separated from the harvested pods which are usually collected manually.
Grading
Like in India and most Asian countries (Radovich 2013), there are no known formal
quality standards and grading for moringa products in Nigeria.
Packaging
The leaves are particularly perishable and are stored under cool temperatures and
high humidity to avoid excessive wilting and leaflet abscission. This is most often
done by bagging in plastics and refrigerating at approximately 10 °C (50 °F), while
the fruit may be canned to preserve it for later consumption (Radovich 2013).
However, in Nigeria, the leaves are mostly preserved by sun-drying and packaging
in polythene bags.
Storage
Moringa oil possesses exceptional oxidative stability and can be stored for years
while maintaining quality. Leaf and pods may be stored at 10 °C (50 °F) for 5–14
days, but intact pods are less perishable than peeled or cut pods, while shelf life of
the seed cake has not been established (Radovich 2013). In Nigeria, the leaf may be
stored and utilized when sun-dried.
Marketing
Pods and especially leaves are suited for local markets, but important new potential
markets include ethnic groups not traditionally using the crop, restaurants, and
health food stores and the West African Sub-region. Leaves, medicinal, and value-
added products have potential for shipment to North America and Asia, while fruits
are not acceptable in continental U.S. because of pest concerns.
However, given the increased awareness of the high nutritional value of moringa,
health food stores may be the most receptive new market in the short term. Moringa
is relatively easy to grow organically and organic certification may increase con-
sumer appeal. Strong potential for internet sales also exist, particularly for oil and
supplements because of product stability and established or emerging markets.
Based on superfood trends, producers of processed juices and smoothies may also
be a potential high-value market for moringa growers (Radovich 2013).
Production levels and schedules differ between different ecological zones in Nigeria,
but generally, leaf fresh weight yield is 1–5 kg (2.2–11 lb) per tree/year, equivalent
to 10,000–50,000 kg/ha (8900–44,534 lb./ac) per year at 1 m × 1 m (3.3 ft. × 3.3 ft)
spacing. Commercial leaf yield of moringa reportedly declines from November–
March to 50–100% of summer yields in the west coast of Hawaii (Radovich 2013)
and many regions of Nigeria, most probably as a result of function of lower solar
radiation during winter/harmattan season. At 10 cm × 10 cm (4 in × 4 in) spacing,
leaf yields are 7–8 kg/m2 (1.4–1.6 lb./ft2) at the first cutting in well irrigated,
drained and fertilized beds, with up to seven cuttings a year.
As per the pods, yields in India are reported at 19 kg (42 lb) pods/tree/year. This
is the equivalent of 31,000 kg/ha (27,600 lb./ac) per year at 2.5 m × 2.5 m (8.2 ft. ×
8.2 ft) spacing. At 230 pods per tree, pods average 80–90 g (2.8–3.2 oz) each. In
Hawai‘i, the Indian variety ‘PKM-2′ yielded 3–8 times more immature pods than
local accessions 6 months after transplanting seedlings (Radovich 2013).
Because the cropping of moringa is an emerging trend in Nigeria, information on
production level are rather scanty. However in Hawai‘i (Radovich 2013), it is
reported to produce about 3 g (0.1 oz) of kernel per dry pod. Oil yield per hectare
per year may be estimated based on the numbers above. Assuming a relatively high
20% oil yield by weight from kernels and a 0.90 specific gravity for the oil, trees
might be expected to produce 250 l of oil per hectare (107 qt/ac).
Part 5: Utilization
Cosmetic
Moringa seeds have been found to be effective against skin-infecting bacteria
Staphylococcus aureus and Pseudomonas aeruginosa. They contain the potent anti-
biotic and fungicide terygospermin. The alkaloid spirachin (a nerve paralysant) has
been found in the roots (Orwa et al. 2009).
Pharmaceutical and Therapeutics
Decoction of the flowers is used as a remedy for cold, while the gum is utilized as a
diuretic, astringent and abortificient, as well as in managing asthma. The oil is used
for hysteria, scurvy, prostate problems and bladder troubles. The roots and bark are
used for cardiac and circulatory problems, as a tonic and for inflammation. The bark
is an appetizer and digestive aid. The iron content of the leaves is high, and they are
reportedly prescribed for anaemia in the Philippines (Orwa et al. 2009). Moringa
has long been known in traditional medicine worldwide as having value both as a
preventative and treatment agent of several health conditions, including the treat-
ment of inflammation, infectious diseases, cardiovascular, gastrointestinal, haema-
tological and hepatorenal disorders.
A number of scientific articles have indeed been published describing the profil-
ing of the antioxidant polyphenols and antioxidant properties of moringa (Atawodi
et al. 2010), which can translate to its use as an anti-ageing herb (Ndhlala et al.
2014). Other medicinal uses of the leaves include treatment of fever, ear infections,
lowering of blood sugar (diabetes mellitus), blood pressure (Stevens et al. 2013) and
trypanosomiasis (Atawodi and Shehu 2010).
Food and Flavoring
The leaves, a good source of protein, vitamins A, B and C and minerals such as
calcium and iron, are used as a spinach equivalent. They are an excellent source of
the sulphur-containing amino acids methionine and cystine, which are often in short
supply. Young plants are eaten as a tender vegetable and the tap roots may be used
as an alternative for horseradish. Young pods are edible and reportedly have a taste
reminiscent of asparagus. Although not very popular in Nigeria, the green peas and
surrounding white material can be removed from larger pods and cooked in various
ways for human consumption in Asian countries. Seeds from mature pods (which
can be 40–50 cm long) can be browned in a skillet, mashed and placed in boiling
water, which causes an excellent cooking or lubricating oil to float to the surface.
The pleasantly flavored edible oil, resembling olive oil, is an excellent salad oil. The
flowers can be eaten or used to make a tea. Leaves are mainly used for human food
and not to any great extent for livestock, but branches are occasionally lopped for
feeding camels and cattle (Orwa et al. 2009).
Industrial
The oil (which is brilliant yellow) content of de-hulled seed (kernel) is approxi-
mately 42%, and it is mainly used as a lubricant for fine machinery such as time-
pieces because it has little tendency to deteriorate and become rancid and sticky. It
is also useful as vegetable cooking and frying oil. The oil, with free fatty acid con-
tent varying from 0.5 to 3%, and containing approximately 13% saturated fatty
acids and 82% unsaturated fatty acids, is popular in the perfume industry for stabi-
lizing scents because of its known capacity to absorb and retain volatile substances.
It has a particularly high level of oleic acid (70%), as compared to other vegetable
oils that contain only about 40% oleic acid (Zaku et al. 2015).
Others
Water Purification
Moringa seeds contain between 30 and 42% oil and the press cake obtained as a
by-product of the oil extraction process contains a very high level of protein. Some
of these proteins (approximately 1%) are active cationic poly-electrolytes having
molecular weights between 7 and 17 kDa. The cationic poly-electrolytes neutralize
the colloids in muddy or dirty water since the majority of these colloids have a nega-
tive electrical charge. This protein can therefore be used as a non-toxic natural poly-
peptide for sedimentation of mineral particles and organics in the purification of
drinking water, for cleaning vegetable oil, or for sedimentation of fibers in the juice
and beer industries. It thus works as a primary coagulant as natural bridges are con-
tinuously formed between the colloid particles (Zaku et al. 2015).
Safety Data
According to Khawaja et al. (2010), moringa is an edible and extremely safe plant.
2 Conclusion
Cultivation of medicinal plants in Nigeria can be considered as a rarity. However,

five medicinal plants namely, Vernonia amygdalina, Cyperus esculentus,
Cymbopogon citrates, Khaya senegalensis and Moringa oleifera were identified
and reviewed with respect to general aspects, cultivation practices, post-harvest

handling, production schedules and utilization. Available literature on the medicinal
plants indicated the presence of phytochemical compounds that exerts pharmaco-
logical and biological effects. However, the fewness of comprehensive literature on
cultivated medicinal plants was a clear indication of total negligence or dearth of
understanding about the need, importance and even the plausibility of cultivating
medicinal plants. Culturally though, plants used for medicinal purposes in most of
Africa including Nigeria are usually harvested from the wild. But with evident rap-
idly depleting natural resources due to over exploitation, urbanization and climatic
changes, the availability and supply of medicinal plants cannot be sustained in the
upcoming few years. As such, cultivation is the only way to ensure availability and
sustenance of supply. So far however, this work has identified a near or total lack of
attention to the issue of medicinal plants cultivation and by extension, conservation
in Nigeria.
Acknowledgments Professor S.E. Atawodi’s research work on antioxidants was partly supported

by Alexander von Humboldt Foundation of Germany and the German Cancer Research Centre
(DKFZ), Heidelberg.
References
Agbogidi OM, Akpomorine MO (2013) Health and nutritional benefits of bitter leaf (Vernonia
amygdalina del.) Int J A PS BMS 2(3):164–170
Akah PA, Okafor CI (1992) Hypoglycaemic effect of vernonia amygdalina del, in experimental
rabbits. Plant Med Res 1:6–10
Akande IS, Samuel TA, Agbazue U, Olowolagba BL (2011) Comparative proximate analysis of
ethanolic and water extracts of Cymbopogon citratus (lemon grass) and fourtea brands. Plant
Sci Res 3(4):29–35
Anderson JW, Smith BM, Gustafson NJ (1994) Health benefits and practical aspects of high fibre
diets. Am J Clin Nutr 59:1242S–1247S
Anon AC (1992) Macropaedia. In: The new encyclopedia britannica, vol 3, 15th edn. Encyclopaedia
Britannica, Chicago, p 185
Aregheore EM (1998) A note on the nutritive value of dry ripe plantain peels as a replacement of
maize for goats. J Anim Feed Sci 7(1):55–62
Aregheore EMK, Makkar HPS, Becker K (1998) Feed value of some browse plants from the cen-
tral zone of Delta State. Nig Trop Sci 38:97–104
Atawodi SE, Shehu H (2010) Anti-trypanosomal property of some extracts of different parts of
Moringa oleifera, lam. Electron J Biol 6(1):19–24
Atawodi SE, Atawodi JC, Idakwo P, Pfundstein B, Haubner R, Wurtele G, Spiegelhalder B, Bartsch
H, Owen RW (2009a) Evaluation of the polyphenol composition and antioxidant activity of
African variety of Dacryodes edulis (G.Don) H.J Lam Fruit. J Med Food 12(6):1321–1325.
doi:10.1089/jmf.2008.0215
Atawodi SE, Atawodi JC, Pala Y, Idakwo P (2009b) Assessment of the polyphenol profile and
antioxidant properties of leaves, stem and root barks of Khaya senegalensis (Desv.) Electron
J Biol 5(4):80–84
Atawodi SE, Atawodi JC, Idakwo GA, Pfundstein B, Haubner R, Wurtele G, Spiegelhalder B,
Bartsch H, Owen RW (2010) Evaluation of the polyphenol content and antioxidant properties
of methanol extracts of the leaves, stem and root barks of Moringa oleifera, lam. J Med Foods
13(3):710–716
Baba MD, Yakubu G, Yelwa JM, Haruna L (2015) Costs and returns of Moringa (Moringa oleifera)
production in Zara local government area of Kebbi state, Nigeria. N Y Sci J 8(1):36–40
Bamishaiye EI, Bamishaiye OM (2011) Tiger nut: as a plant, its derivatives and benefits. Afr
J Food Agric Nutr and Dev (AJFAND) Online, 11(5):5157 September 2011
Belewu MA, Abodunrin AO (2006) Preparation of Kunnu from unexploited rich food source: tiger
nut (Cyperus esculentus). World J Dairy Food Sci 1:19–21
Biggelaar CD, Gold MA (1996) Development of utility and location indices for classifying agro-
forestry species: the case of Rwanda. Agrofor Syst 34:229–246
Blanco MM, Costa CA, Freire AO, Santos JG, Costa M (2009) Neurobehavioural effects of essen-
tial oils of Cymbopogon citratus in mice. Phytomedicine 16:265–270
Burkill HM (1985) The useful plants of West tropical Africa, vol 3, 2nd edn. Royal Botanical
Gardens, Kew
Carianne dB (2005) Organic lemongrass, a guide for smallholders. EPOPA (Export Promotion of
Organic Products from Africa), Bennekom, pp 1–27
Chweya JA, Eyzaguirre P (1999) The biodiversity of traditional leafy vegetables. IPGRI, Rome,
p 182
Dansi A, Adjatin A, Adoukonou-Sagbadja H, Akpagana K (2008) Production and traditional seed
conservation of leafy vegetables in Benin rural areas. Bulletin de la Recherche Agronomique
du Bénin, Numéro 59:59–69
Dike MC (2010) Proximate, phytochemical and nutrient compositions of some fruits, seeds and
leaves of some plant species at Umudike, Nigeria. ARPN J Agric Biol Sci 5(1):7–16
Directorate [Communication Services] (2012) Essential oil crops: production guidelines for lemon
grass. Department of Agriculture, Forestry and Fisheries, Republic of South Africa. www.daff.
gov.za/publications. Accessed 11 Apr 2015
Dyer AR (2009) The ecology of chufa (Cyperus esculentus sativus). Department of Biology &
Geology University of South Carolina Aiken
Ekpenyong CE, Akpan EE, Daniel NE (2014) Phytochemical constituents, therapeutic applica-
tions and toxicological profile of Cymbopogon citratus Stapf (DC) leaf extract. J Pharmacogn
Phytochem 3(1):133–141
Ene AC, Atawodi SE, Ameh DA, Nnamani CN, Apeh YEO (2009) Antitrypanosomal effects of
petroleum ether, chloroform and methanol extracts of Artemisia maciverae Linn. Ind J Exp
Biol 47:981–986
FAO (1988) Traditional food plants: a resource book for promoting the exploitation and consump-
tion of food plants in arid, semi-arid and sub-humid lands of Eastern Africa, vol 42. FAO Food
and Nutrition Paper, Rome, pp 239–242
FAO (1998). Cyperus esculentus. AGRIS. www.agris.fao.org. Accessed 26 May 2015
FAO/WHO (1985) Energy and protein requirement. report of a joint FAO/WHO/UNU expert con-
sultation, WHO Technical Report Series No. 724, Geneva
Foidl N, Makkar HPS, Becker K (2016) Planting and care instructions – for your moringa seeds
and trees Moringa potential and info. http://www.moringaseeds.co.za/Planting3
Getaneh S, Girma Z (2014) An ethnobotanical study of medicinal plants in Debre Libanos Wereda,
Central Ethiopia. Afr J Plant Sci 8(7):366–379
Halabi MF, Sheikh BY (2014) Anti-proliferative effect and phytochemical analysis of Cymbopogon
citratus extract. Bio Med Res Intl, Article ID 906239. http://dx.doi.org/10.1155/2014/906239
Hamowia AM, Saffaf AM (1994) Pharmacological studies on Vernonia amygdalina (Del) and
tithonia diversifolia (Gray). Vet Med J2:91–97
Ijeh II, Akomas SC (2005) Effect of oral administration of ethanolic extract of Vernonia amygda-
lina on the exocrine functions in weaning rabbits. In: Proceedings of 30th conference Nigerian
Society for Animal Production 30: 109–112
Ijeh II, Ejike CC (2011) Current perspectives on the medicinal potentials of Vernonia amygdalina
Del. J Med Plant Res 5(7):1051–1061
Izevbigie EB (2003) Discovery of water-soluble anticancer agents (edotides) from a vegetable
found in Benin City, Nigeria. Exp Biol Med 228:293–298
Karkala M, Bhushan B (2014) Review on pharmacological activity of Cymbopogon citratus. Int
J Herb Med 1(6):5–7
Kayode J (2004) Eco-physiological and conservation studies on Vernonia amygdalina in Ekiti
State, Nigeria. Pak J Sci Ind Res 47:227–230
Kemka-Evans CI, Okoli B (2013) Chromosome numbers and karyotype in three species of the
genus Vernonia Schreber in Southern Nigerian. Afr J Plant Sci 7(11):538–542
Khalafalla MM, Abdellatef E, Daffalla HM, Nassrallah AA, Aboul-Enein KM, Lightfoot David
A, Cocchetto A, El-Shemy HA (2009) Antileukemia activity from root cultures of Vernonia
amygdalina. J Med Plants Res 3(8):556–562
Khawaja TM, Tahira M, Ikram UH (2010) Moringa oleifera: a natural gift-a review. J Pharm Sci
Res 2(11):775–781
Kubmarawa D, Khan ME, Punah AM, Hassan M (2008) Phytochemical screening and antimi-
crobial efficacy of extracts from Khaya senegalensis against human pathogenic bacteria. Afr
J Biotechnol 7(24):4563–4566
Liman ML, Atawodi SE (2015) Hepatoprotective and nephroprotective effects of methanol extracts
of different parts of Tamarindus Indica linn in rats following acute and chronic carbon tetra-
chloride intoxication. Ann Res Rev Biol 5(2):109–123
Mann A, Gbate M, Umar AN (2003) Medicinal and economic plants of Nupeland, 1st edn. Jube-
Evans Books and Publications, Bida, p 158
Mpondo ME, Dibong SD (2011) Medicinal plant knowledge of ethnic groups in Douala town,
Cameroon. Am J Food Nutr 1(4):178–184
Ndhlala AR, Mulaudzi R, Ncube B, Abdelgadir HA, du Plooy CP, Staden JV (2014) Antioxidant,
antimicrobial and phytochemical variations in thirteen Moringaoleifera lam. cultivars.
Molecules 19:10480–10494
Nikiema A, Pasternak D (2008) Khaya senegalensis (Desr.) A. Juss. [Internet] Record from
PROTA4U. http://www.prota4u.org/search.asp. Accessed 24 May 2015
Nmor EI, Odeh OH (2013) Effect of tillage on the yield of bitter leaf (Vernonia amydalina) in
Southern Nigeria. J Agric Vet Sci 5(1):159–164
Ojiako OA, Nwanjo HU (2006) Is Vernonia amygdalina hepatotoxic or hepatoprotective? Response
from biochemical and toxicity studies in rats. Afr J Biotechnol 5:1648–1651
Okafor JNC, Mordi JI, Ozumba AU, Solomon HM, Olatunji O (2003) Preliminary studies on the
characterization of contaminants in tiger nut (Yellow variety). In: Proceedings of 27th annual
Nigerian Institute of Food Science and Technology (NIFST) conference, 13–17 October,
pp 210–211
Oloyede GK, Sunday FA, Charles CN (2014) Antioxidant and toxicity screening of extracts
obtained from Cyperus esculentus. Academ Arena 6(1):77–83
Onu A, Saidu Y, Ladan MJ, Bilbis LS, Aliero AA, Sahabi SM (2013) Effect of aqueous stem bark
extract of Khaya senegalensis on some biochemical, haematological and histopathological
parameters of rats. J Toxicol. http://dx.doi.org/10.1155/2013/803835
Onwuka CFI, Akinsoyinu AO, Tewe OO (1989) Feed value of some Nigerian browse plants: chem-
ical composition and in vitro digestibility. East Afr Agric For J 54:157–163
Orwa C, Mutua A, Kindt R, Jamnadars R, Anthony S (2009) Agroforestree database: a tree refer-
ence and selection guide version 4.0. http://www.worldagroforestry.org/sites/treedbs/treedata-
bases.asp. Accessed 5 May 2015
Osagie AU, Eka OU (1998) Nutritional quality of plant foods. Post-harvest Research Unit 246–249
Osagie AU, Okoye WI, Oluwayose BO, Dawodu OA (1986) Chemical quality parameters and fatty
acid position of oils of under exploited tropical seeds. Nig J Appl Sci 4:151–162
Paliwal R, Sharma V, Pracheta V (2011) A Review on horse radish tree (Moringa oleifera): a mul-
tipurpose tree with high economic and commercial importance. Asian J Biotech 3(4):317–328
Parrotta JA (1993) Moringa oleifera Lam. – Reseda, horseradish tree, SO-ITF-SM-61, pp 1–6
Radovich T (2013) Farm and forestry production and marketing profile for moringa
(Moringaoleifera). Specialty Crops for Pacific Island Agroforestry http://agroforestry.net/scps
Raza M, Shah SM (2011) Conservation and sustainable use of medicinal plants in Baltistan. Grace
Association, Pakistan
Sale M, De N, Doughari JH, Pukuma MS (2008) In vitro assessment of antibacterial activity of
bark extracts of Khaya senegalensis. Afr J Biotechnol 7(19):3443–3446
Soares MO, Alves RC, Pires PC, Beatriz M, Oliveira PP, Vinha AF (2013) Angolan
Cymbopogoncitratus used for therapeutic benefits: nutritional composition and influence
of solvents in phytochemicals content and antioxidant activity of leaf extracts. Food Chem
Toxicol 60:413–418
Stevens GC, Baiyeri KP, Akinnnagbe O (2013) Ethno-medicinal and culinary uses of Moringa
oleifera Lam. in Nigeria. J Med Plants Res 7(13):799–804
Tadesse A, Gebre-Hiwot A, Asres K, Djote M, Frommel D (2008) The in vitro activity of Vernonia.
Int J Environ Res Public Health 5(5):341
Tetteh JP, Ofori E (1998) A baseline survey of tiger nut (Cyperus esculentus) production in the
Kwahu South District of Ghana. Ghana J Agric Sci 31:211–216
Ucheck FF (2004) Vernonia amygdalina Delile. http://www.prota4u.org/search.asp. Accessed 1
Apr 2015
Wakirwa JH, Idris S, Madu SJ, Dibal M, Malgwi T (2013) Assessment of the in-vitro antimicrobial
potential of Khaya senegalensis ethanol leaf extract. J Chem Pharm Res 5(7):182–186
Wijenayake T (2014) Khaya: the wonder tree for commercial cultivation. http://www.ft.lk/arti-
cle/337136/Khaya. Accessed 5 May 2016
Yedjou C, Izevbigie E, Tchounwou P (2008) Preclinical assessment of Vernonia amygdalina
leaf extracts as DNA Damaging Anti-Cancer Agent in the management of breast cancer. Int
J Environ Res Public Health 5(5):337–341
Zaku SG, Emmanuel S, Tukur AA, Kabir A (2015) Moringa oleifera: an underutilized tree in
Nigeria with amazing versatility: a review. Afr J Food Sci 9(9):456–461
The Halophytic Genus Zygophyllum
and Nitraria from North Africa:
A Phytochemical and Pharmacological
Overview
Soumaya Bourgou, Wided Megdiche, and Riadh Ksouri
Abstract In arid and semi-arid regions, plants are often subjected to severe envi-
ronmental conditions such as salinity, drought and extreme temperatures. Halophyte
species are salt-tolerant plants which are characterized by high physiological plas-
ticity and are able to thrive under stressful conditions by developing adaptive
responses including the synthesis of several bioactive molecules such as terpenoids,
phenolic compounds, sterols, polysaccharides, carotenoids, vitamins and glyco-
sides. These primary and secondary metabolites possess potent biological activities
including antioxidant, antiviral, antimicrobial, anti-inflammatory, and anticancer
ones and epidemiological research suggests that these compounds may help prevent
several chronic diseases, such as diabetes, chronic inflammation, atherosclerosis
and cardiovascular disorders. Because of their high content on these bioactive
metabolites, halophytes may offer a valuable source of healthy products and could
be used as functional foods and nutraceuticals. This chapter deals with the ethno-
pharmacological uses of two famous halohyte genus; Zygophyllum and Nitraria in
traditional medicine and reviews recent investigations on their phytochemical com-
positions and biological activities.
Keywords Halophytes • Zygophyllum • Nitraria • Secondary metabolites •

Biological activities
1 Introduction
Halophytic plants are salt-tolerant species able to thrive under stressful conditions
and to survive and to reproduce in environments where salt concentration reaches or
exceed sea water salinity levels. There are more than 2600 halophytic species known
S. Bourgou • W. Megdiche • R. Ksouri (*)

Laboratoire des Plantes Aromatiques et Médicinales, Centre de Biotechnologie de Borj-
Cédria (CBBC), BP 901, Hammam-Lif 2050, Tunisia

DOI 10.1007/978-94-024-1120-1_13
346 S. Bourgou et al.
worldwide to possess salinity tolerance including edible, fodder, fuel, medicine,

chemicals, and ornamentals plants (Abdelly et al. 2011). Halophytes are potentially
useful for ecological applications in arid and semi-arid regions, such as soil desal-
inisation, dune fixation, phyto-remediation, landscaping and ornamentation
(Abdelly et al. 2011).
Halophytes have been collected since ancient times for alimentation and for their
medicinal qualities and until now, a great number of medicinal halophytes are used
to treat various diseases, infections and aging processes particularly in the rural
areas, where the traditional folk medicine remains a major source to cure minor ail-
ments. Currently, an increasing interest is granted to halophytic species because of
their high content in bioactive secondary molecules with a big economic interest
(Ksouri et al. 2012).
Compared to the glycophytic species, halophytes contain much higher amounts
of secondary metabolites or even restricted to halophytic species which could be
used in various application fields such as in the Agri-food, pharmaceutical and cos-
metic industries. The biological function of bioactive compounds might be single,
or combined with others present in whole plant extracts mixtures leading to syner-
gistic effect and depending on its chemical composition and efficacy (Subhashini
et al. 2013). This contribution focuses on the ethno-pharmacological uses of two
famous and interesting genus of halophytes (Zygophyllaceae family) in traditional
medicine and reviews recent investigations on their chemical composition and bio-
logical activities.
2 Zygophyllum Genus
Uses in Traditional North Africa Medicine
Zygophyllum genus comprises several spontaneous species distributed in arid

regions of Mediterranean basin, in southern, northern and north-eastern Africa
(White 1983) and widely used as natural remedies in folk medicine. Zygophyllum
species are halophyte plants widely found along saline and gypsophile areas (Chaieb
and Boukhris 1998). One of the most studied species is Z. album L. which is an
edible succulent dwarf shrub of pharmacological interest. This halophyte has long
been used in traditional Africa medicine against rheumatism, gout and asthma. The
aerial parts of the plant are used as an anti-diabetic agent. It is also used as diuretic
and local anaesthetic and antihistaminic. Moreover, Z. album has been used as
herbal medicine in southern Tunisia to treat diabetes mellitus (Mnafgui et al. 2012).
In Algeria, Z. album L. is employed against aches, diabetes, dermatitis, spasms, and
dysmenorrhoea and for calm thirst as well as for wound care and the treatment of
dental caries (Tigrine-Kordjani et al. 2006; Ayad et al. 2012). Some Bedouin used it
as hay or added it to the dry ration (Daoud and Al-Rawi 1985). In Morocco, the
endemic Zygophyllum gaetulum is used in folk medicine for the treatments of
diabetes and stomach and liver pain. It has antispasmodic, anti-inflammatory,
The Halophytic Genus Zygophyllum and Nitraria from North Africa… 347
Table 1 Chemical constituent of some species of Zygophyllum from North Africa

Plant
Species Origin part Chemical constituents References
Z. album Egypt shoots flavonol 3-O-rutinosides, Hussein et al.
isorhamnetin 3-O-glucosides (2011)
Z. album Tunisia shoots malvidin 3-rhamnoside, Megdiche Ksouri
quercetin-3-sulphate, et al. (2013)
isorhamnetin-3-O-rutinoside,
triterpenes saponinssterols
Z. album Algeria shoots essential oil Tigrine-Kordjani
et al. (2006)
Z. berenicense flavonol 3-O-rutinosides Saleh and
El-Hadidi (1977)
Z. corntum Algeria whole isorhamnetin-3-O-rutinoside Ayad et al. (2012)
plant β-sitosterol
Z. coccineum Egypt roots flavonol 3-O-rutinosides, Saleh and
triterpenes saponins El-Hadidi (1977)
and Pöllmanns
et al. (1997)
Z. dumosum Egypt roots triterpenes saponins Pöllmanns et al.
(1997)
Z. simplex Egypt shoots flavonol 3,7-diglycosides, Hussein et al.
gentisic acid, gentisic acid (2011)
5-O-α-rhamnopyranoside
Z. gaetlum Maroc roots zygophyloside N, quinovic acid Aquino et al.
glycosides (2001)
anti-diarrhoeal and anti-eczema effects (Bellakhdar et al. 1991; Ait El-Cadi et al.
2008). In addition, Z. fabago roots are also used as an antispasmodic remedy as an
alternative to the aerial parts in Moroccan folk medicine (Aquino et al. 2001). The
leaves and the seeds of Zygophyllum simplex are used in Egypt as anthelmintic and
applied to eyes in cases of Ophthalmia and Leucoma (Mahmoud and Gairola 2013).
Z. coccineum is the most widespread Zygophyllum species in Egypt, common in the
desert and the saline soils. It is used as a drug against rheumatism, gout and hyper-
tension (Pöllmanns et al. 1997).
Phytochemical Composition
Several studies reported the separation and identification of flavonoids from some
Zygophyllum species (Table 1). For instance, Quercetin-3-O-rutinoside,
isorhamnetin-3-O-β-glucopyranoside, isorhamnetin-3-O-rutinoside were detected
by Saleh and El-Hadidi (1977) in Z. decumbens. Recently, Hussein et al. (2011)
from Egypt isolated from 70% methanol extract of Z. album several flavonoids:
quercetin-3-O-rutinoside [Quercetin-3-O-α-rhamnopyranosyl-(1 → 6)-O-β-
glucopyranoside], isorhamnetin-3-O-β-galactopyranoside, isorhamnetin-3-O-β-
glucopyranoside, isorhamnetin-3-O-rutinoside [isorhamnetin-3-O-α-rhamnopyranosyl-
(1/6)-O-β-glucopyranoside], isorhamnetin 3-O-robinoside [isorhamnetin-3-O-α-

rhamnopyranosyl-(1/6)-O-β-galactopyranoside]. These compounds were identified
by Rf values, colour reactions, UV spectrophotometry, 1H- and 13C-NMR spec-
trometery and co-chromatography. Previous investigation of Z. album revealed
others compounds such as zygophyllin, β-sitosterol-β-D-glucopyranoside, carbo-
hydrates, tannins, lactones, protein/amino acids, saponins, terpene and flavonoid
glycosides (Hani et al. 1995; Atta and Mouneir 2004). In Z. simplex from
Egypt, quercetin, quercetin-3,7-di-O-β-glucopyranoside, quercetin-3,7-di-O-β-
glucopyranoside, isorhamnetin-3- O-β-glucopyranoside-7-O-α-rhamnopyranoside,
gentisic acid and gentisic acid 5-O-α-rhamnopyranoside were purified by Hussein
et al. (2011). Isorhamnetin-3-O-rutinoside was found to be a chemotaxonomic
marker in the genus of Zygophyllum. This compound was reported as one of the
major phenolic compounds in the aerial part of Z. album grown in Tunisia by
Megdiche et al. (2013) along with two other flavonoid molecules: malvidin 3-rham-
noside and quercitin-3-sulphate. Interestingly, these authors showed among 10
compounds identified by liquid chromatography coupled to electrospray time-of-
flight mass spectrometry (LC-TOF/MS) such as saponins, flavonoids and sterols,
five were reported for the first time in Z. album shoot extracts. Isorhamnetin-3-O-
rutinoside was reported also by Ayad et al. (2012) from Z. cornutum endemic to
Algeria. Zygophylum is a saponin-rich genus (Table 1). In Z. gaetulum from
Maroc, fractionation of methanolic extract led to the isolation of the saponin
zygophyloside N [3-β-O-α-l-rhamnopyranosyl-(1-2)-O-α-l-arabinopyranosyl-(1->2)-
β-d-glucopyranosyl)oxy]-ursan-20β,28-olide], together with quinovic acid glyco-
sides: zygophyloside G (2), zygophyloside E and 4, 3-O-β-D-glucopyranosyl
quinovic acid 28-β-d-glucopyranosyl ester (Aquino et al. 2001). In addition, zygo-
phyloside G (3-O-[β-D-2-O-sulphonylglucopyranosyl]-quinovic acid-28-O-[β-D-
glucopyranosyl] ester have been isolated from the roots of Z. dumosum (Ahmad
et al. 1993), Z. coccineum (Pöllmanns et al. 1997) and Z. album (Megdiche Ksouri
et al. 2013). Pöllmanns et al. (1997) investigated the crude methanolic saponin
extracts from Egyptian Z. coccineum and Z. dumosum roots and purified triterpenes
saponins zygophyloside H, 3-0-[β-D-glucopyranosyl]-quinovic acid-28-0-[β-D-
glucopyranosyl] ester as well as zygophyloside K. Recently, zygophyloside K have
been identified by Megdiche et al. (2013) in Tunisian Z. album. Recently, phyto-
chemical analysis of Z. coccineum have revealed that the major secondary metabo-
lites are a class of quinovic acid compounds belonging to the ursane-type triterpene
saponins (Amin et al. 2011). Tigrine-Kordjani et al. (2011) extracted the essential
oil from Zygophyllum album L., growing in Ouargla Sahara region (Algeria) by
hydro-distillation. They reported a strong musk flavour and (E)-β-damascenone
(11.8%) chemotype. Other major compounds of Z. album essential oil were charac-
terized δ-decalactone (7.8%), α-inone (7.1%), 3-nonen-2-one (4.6%), safranal
(3.4%),(E)-2-hexen-1-ol (3.8%), (E)-caryophyllene (1.6%) and cis-linalool oxide
(1.3%). On the other hand, fractionation of the ethyl acetate extract of Z. cornutum
resulted in the isolation of β-sitosterol (Ayad et al. 2012 ). In Z. album from Tunisia,
Megdiche et al. (2013) identified 3-O-[β-D-glucopyranosyl]-β-sitosterol and
β-sitosterol-β-D-glucoside by comparison of their spectral and physical data with

those of known compounds. 3-O-[β-D-glucopyranosyl]-β-sitosterol seems to be a
chemotaxonomic marker in the genus of Zygophyllum (Ahmad et al. 1993;
Hassanean and Desoky 1992; Hassanean et al. 1989; Hassanean et al. 1989).
The most common complementary and alternative medicine modalities are based in
food supplements and herbal therapies. In this regard, several plant extracts of
Zygophyllaceae family and their isolated bioactive compounds were shown to be
effective in prevention and treatment of some diseases.
(i) Antioxidant capacity A number of pharmacological and clinical studies have
been reported from different Zygophylum species which proves the potentiali-
ties and the importance of these plants. Megdiche et al. (2013) evaluated the
antioxidant activity of the hexane, dichloromethane and methanol extracts of
Tunisian Z. album shoots in vitro using the ORAC assay, as well as, ex-vivo
using a cellular based-assay using DCFH-DH, a useful indicator of reactive
oxygen species (ROS). The most polar extract (methanol) had a strong antioxi-
dant ORAC capacity (1.19 ± 0.07 μmol Trolox/mg) and the dichloromethane
extract proved the utmost antioxidant activity in cell (WS1) based-assay
(IC50 = 57 μg/ml). Additionally, El Ghoul et al. (2012) have also observed that
the administration of Z. album aqueous extract to diabetic mice induced a dim-
inution in the lipid peroxidation in the liver and the pancreas in a dose depen-
dent manner, which was connected to an enhancement of the enzymatic (GSH)
and non-enzymatic (vitamin C) anti-oxidative scavengers.
(ii) Anti-diabetic activity Aqueous and ethanol extracts of Z. album from Tunisia
exerts anti-diabetic and anti-hyper-cholesterolemic activities. Oral administra-
tion of the extracts reduced the blood glucose, total cholesterol (TC), triglycer-
ides (TG), low-density lipoprotein (LDL) and very low-density lipoprotein
(VLDL) levels in treated diabetic mice in comparison with control group. Z.
album extracts prevents the diabetic induced MDA levels via the enhancement
of the tissue glutathione reductase (GSH), blood vitamin C levels, superoxide
dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) (El Ghoul
et al. 2011, 2012). The ability of Z. gaetulum leaves from Morocco to lower the
blood glucose was studied in several patients with non-insulin-dependent dia-
betes mellitus (Jaouhari et al. 1999). Z. gaetulum infusion possesses significant
oral anti-hyperglycaemic activity by lowering plasma glucose levels. The anti-
hyperglycaemic effect was seen both after acute and sub-acute administration
of Z. gaetulum in non-insulin-dependent diabetics patients. Moreover, the tox-
icity study shows that large dose of the extract can be administered without
hazard.
(iii) Anti-diarrhoeal activity Ait El Cadi et al. (2008) have shown that Z. gaetulum
infusion possess anti-diarrhoeal activity in mice. The extract was prepared
according to traditional methods and the study was carried out in vitro using
chamber. Atta and Mouneir (2004) investigated the antidiarrhoeal activity of
six Egyptian medicinal plant extracts and their effect on motility of isolated
rabbit’s duodenum. The study showed that oral administration of methanol
extract obtained from the shoots of Z. album grown in Egypt induced anti-
diarrhoeal effect against castor oil-induced diarrhoea. The anti-diarrhoeal
effect of the plants could be attributed, at least partially to their content of tan-
nins which form protein tannate that reduces intestinal secretion.
(iv) Anti-inflammatory ability In order to approve the traditional use of Z. gaetu-
lum in Moroccan folklore as anti-inflammatory agent, Ait El Cadi et al. (2012)
compared the anti-inflammatory effect of ethanolic extract obtained in soxhlet
apparatus with aqueous extract obtained by infusion, and the anti-inflammatory
activity was estimated by measuring the oedema induced by carragenin.
Extracts of Z. gaetulum reduced the increase of the paw volume which sug-
gests that the aqueous and ethanolic extracts could be used scientifically in the
treatment of inflammation. Moreover, Megdiche et al. (2013) evaluated after
fractionation by successive soxhlet extraction using solvents with increasing
polarity, the anti-inflammatory capacity of the extracts obtained from Z. album
grown in Tunisia using LPS-induced RAW 264.7 macrophages. The results
showed that methanol extract displayed the best inhibition of NO release, in a
dose-dependent manner. Thus, inhibition was ranging from 19.4 (20 μg/ml) to
84.8 % (160 μg/ml). In comparison, L-NAME, as positive control, inhibited
NO release by 26.2%. Such important anti-inflammatory activity of could be
due essentially to the richness of extracts on phenolic compounds including the
quercetin 3-sulphate and isorhamnetin-3-O-rutinoside as well as triterpenoid
saponins.
(v) Anticancer power In Z. album from Tunisia, Megdiche et al. (2013) evaluated
the anti-proliferative capacity of the methanol, ethyl acetate and water frac-
tions obtained from shoot crude extract. The cytotoxicity of each extract was
assessed against Human colon carcinoma (DLD-1) and lung carcinoma
(A-549) cell lines, as well as, health skin fibroblast cell line (WS1). Result
showed that among the three shoot fractions, the dichloromethane extract was
significantly active against the two carcinoma cells (A-549 and DLD-1), with
important IC50 values, respectively of 37 and 48 μg/ml. In fact, Z. album had an
influence on tumour cell viability and targeted colon and lung carcinoma cell
lines which could be attributed to the powerful cytotoxic compounds in dichlo-
romethane fraction mainly triterpenes saponins and sterols. The same extract
with dichloromethane was not significantly cytotoxic against fit human skin
fibroblast cell (WS1). In addition, trypan blue assay was used for the evalua-
tion anticancer activity of ethanolic and aqueous Egyptian Z. simplex extracts
against Ehrlich Ascites Carcinoma Cells (EACC). The results displayed that
the extracts gave anticancer activity more than 60% inhibition of proliferation
(Aboul-Enein et al. 2012).
3 Nitraria genus
Uses in Traditional North Africa Medicine
Nitraria species are widely distributed around the world; they are well adapted to
arid climates, and some of them grow in the deserts and salt marshes of Africa, in
northern and occidental parts of the Sahara desert, and in Mauritania (N. tridendata
and N. retusa). These species can also be found in Algeria, Mauritania, Senegal, and
Saudi Arabia. A decoction of fresh leaves of N. retusa is used in Morocco in case of
poisoning, upset stomach, ulcers, gastritis, enteritis, heartburn, colitis and colonic
abdominal pain (Bellakhdar 1997). In Tunisia, it is widespread in central and south
parts. This salt-tolerant and drought resistant shrub grows along shallow and hum-
mocks on saline grounds near the coastal areas and produces fleshy red fruits from
which a tasty and refreshing juice may be extracted. Nitraria retusa is known in
Tunisia as “Ghardaq”. Its leaves and twigs are occasionally grazed by sheep, goats
and camels (Le Houérou 1980). The woods are used as fuel by the local inhabitants.
The sweet drupes are edible and are used to prepare drinks (Daoud and Al-Rawi
1985; Tackholm 1974). Fruits of Nitraria species have been used as nutritional food
and traditional herb for the treatment of hypertension and abnormal menstruation
(Hadj Salem et al. 2011). The leaves serve as supplement for the tea and are used as
poultice (Boubaker et al. 2011). The ashes of this species have the ability to remove
fluids of infected wounds. The leaves infusion (used as cataplasm) has anti-
inflammatory property (Chaieb and Boukhris 1998). The ashes of this species have
the ability to remove fluids of infected wounds. In Egypt, it is known locally as
Ghardaq and used by the Bedouins as a source of fuel and the sweet drupes are
edible (Halim Ahmed et al. 1995).
Phytochemical Composition
The survey involving N. retusa indicated that flavonol glycosides are predominant
(Zar Kalai et al. 2014; Saleh and El-Hadidi 1977). From the leaves and young stems
of N. retusa grown in Egypt, Halim Ahmed et al. (1995) isolated and fully character-
ized six flavonol glycosides: isorhamnetin 3-O-4-rhamgalactosylrobinobioside,
isorhamnetin 3-robinobioside, isorhamnetin 3-rutinoside, isorhamnetin
3-galactoside, isorhamnetin 3-glucoside, isorhamnetin 3-xylosylrobinobioside and
free isorhamnetin. Hussein et al. (2009) isolated and identified twelve flavonoid
compounds from the hole plant N. retusa ethanol extract: (1) apigenin-6-C-
arabinopyranoside-8-C-glucopyranoside, (2) apigenin-6,8-di-C-glucopyranoside,
(3) luteolin-7-O-glucopyranoside, (4) luteolin-7-O-xylopyranosylglucopyranoside,
(5) kaempferol-3-O-xylopyranosyl-(1 → 2)-[O-rhamnopyranosyl-(1 → 6)]-O-
galactopyranoside, (6) free isorhamnetin, (7) isorhamnetin-3-O-galactopyranoside,
(8) isorhamnetin-3-O-glucopyranoside, (9) isorhamnetin-3-O-rutinopyranoside,
(10) isorhamnetin-3-O-robinopyranoside, (11) isorhamnetin-3-O-β-xylopyranosyl-

(1 → 2) [O-rhamnopyranosyl-(1 → 6)]-O-galactopyranoside and (12) isorhamne-
tin-3-O-rhamnopyranoside-7-O-glucopyranoside. Recently, Hadj Salem et al.
(2011) identified by LC–ESI-MSn, four major flavonoids in water and methanol
extracts/fractions of the leaves of N. retusa collected from Tunisia: isorhamnetin,
isorhamnetin-3-O-glucoside, isorhamnetin-3-O-rutinoside and isorhamnetin-3-O-
robinobioside. The chromatographic profile of Tunisian N. retusa extract, based on
the HPLC analysis, acquired at 254 nm displayed three main components: querce-
tin, isorhamnetin-3-O-glucoside, and isorhamnetin-3-O-rutinoside (Zar Kalai et al.
2013). In addition, the quantification of these phenolic compounds were: 22.34 μg/
ml (quercetin), 331.42 μg/ml (isorhamnetin-3-O-glucoside), and 24.91 μg/ml (isor-
hamnetin-3-O-rutinoside). Using GC/MS analysis, Mohamed et al. (2014) detected
eugenol, germacrene D, tetratetracontane Tetratetracontane Hentriacontane as the
major compounds in petroleum ether extracts of N. retusa shoots collected from
various regions in Egypt.
(i) Antioxidant capacity Hadj Salem et al. (2011) investigated the in vitro anti-
oxidant activity of four Tunisian N. retusa extracts obtained by successive
extractions performed with a Soxhlet apparatus with increasing polarity sol-
vents (hexane, chloroform, ethyl acetate and methanol) as well as their frac-
tions. The authors showed that shoot extracts exhibit high antioxidant and
radical scavenging and xanthine oxidase inhibition activities which derive
from the richness of extracts on isorhamnetin-based flavonoids (isorhamnetin,
isorhamnetin-3-O-glucoside, isorhamnetin-3-O-rutinoside and isorhamnetin-
3-O-robinobioside). Besides, Boubaker et al. (2010) have demonstrated an
antioxidant protective role of hexane, and chloroform extracts prepared from
the leaves of Tunisian N. retusa against DNA strand scission induced by
hydroxyl radical. The addition of chloroform extract induced a dose dependant
DNA protection to the damage of native super-coiled circular DNA. Whereas,
Hexane extract showed a low inhibiting activity against hydroxyl radicals. The
scavenging potential for hydroxyl radicals of chloroform extract was found to
be correlated to its polyphenol and sterol contents. When comparing the anti-
oxidant activity of various solvent extracts, Chaâbane et al. (2014) demon-
strated that the water aqueous extract possess the best antioxidant activity
exhibiting high antiradical power and remarkable inhibitory effect on lipid
oxidation. In fact, the last extract displayed the highest amounts of phenolics,
flavonoids and anthocyanins.
(ii) Anti-obesity effect Obesity is a problem of growing importance, being an
essential risk factor for type-2 diabetes, when accompanied with other known
complications. Therefore, it has been an important therapeutic goal to reduce
the risk of type-2 diabetes through weight management. In recent study, Zar
Kalai et al. (2013) investigated the anti-obesity effects of Nitraria retusa etha-
nol extract in 3T3-L1 cells using different doses (25, 50, 100, 200, and 400 𝜇g/
mL). Results showed that ethanol extract could inhibit the lipid droplet accu-
mulation compared to untreated cells, in dose dependent manner. The triglyc-
eride accumulation significantly decreased to 42.70 ± 2.10% at 400 𝜇g/mL. In
fact, they noticed that N. retusa treatment in 3T3-L1 was accompanied by
modulation of cell hypertrophy rather than cell hyperplasia. Moreover, Zar
Kalai et al. (2014) studied the prevention and controlling weight gain effect of
Tunisian N. retusa ethanolic extract using high fat diet-induced obesity experi-
ment in C57B6J/L mice. The results showed that oral administration of etha-
nolic N. retusa extract significantly suppress increases in body and fat mass
weight, decreases triglycerides and LDL-cholesterol levels and enhances gene
expression related to lipid homeostasis in liver showing anti-obesity actions.
The findings, indicate that N. retusa possesses potential anti-obesity effects in
BKS.Cg-Dock7m+/+ Leprdb/J model mice and may relieve obesity-related
symptoms including hyperlipidemia through modulating the lipolysis–lipo-
genesis balance.
(iii) Antimutagenic activity Chloroform extract obtained from Tunisian N. retusa
showed an effective antimutagenic effect, preventing mutations induced by
various oxidants in Salmonella typhimurium TA102 and TA 104 strains
(Boubaker et al. 2010). The authors found that this antimutagenic capacity was
associated to a strong antioxidant power of the extract and could be linked to
the high flavonoids content since they provide strong nucleophilic centers,
which enable them to react with electrophilic mutagens and form adducts that
may result in the prevention of genotoxic damages. N. retusa hexane extract
exhibited also antimutagenic effect which has been found to be correlated to
high sterol content. The authors suggest that consumption of N. retusa could be
an alternative for reducing genotoxic damage induced by free radicals.
(iv) Anticancer activity Hadj Salem et al. (2011) have observed that N. retusa
leaves extracts and their fractions, especially ethyl acetate fraction, were effec-
tive towards Caco-2 cancer cells. Moreover, a correlation between the anti-
proliferative activities of extracts and their flavonoid content was established;
the most concentrated extracts/fractions were the most active. In addition,
when tested as references, isorhamnetin and isorhamnetin-3-O-rutinoside
exhibited high anti-proliferative activities on Caco2 cells. According to
Boubaker et al. (2011) the leaf extracts of N. Retusa have a significant anti-
proliferative effect on the human chronic myelogenous erythroleukaemia
(K562). They induced in a time-dependent manner the apoptosis in cancerous
cell line. This result was confirmed by ladder DNA fragmentation profile and
PARP cleavage, as well as a release in caspase-3 and caspase-8 level.
4 Conclusion
Halophyte species as edible and folkloric medicinal plants have not yet received
much attention as sources of bioactive molecules due to limited popularity or lack
of commercial applications. However, recent investigations clearly demonstrate the
appreciable potentialities of these salt tolerant species that are a rich source of phy-
tochemicals including various phenolic compounds and offer opportunities for
development of value-added products for nutraceutical and food applications as
health promoting factors.
Acknowledgments This study was supported by the Tunisian Ministry of Higher Education and
Scientific Research.
References
Abdelly C, Debez A, Smaoui A, Grignon C (2011) Halophyte-fodder species association may

improve nutrient availability and biomass production of the sabkha ecosystem. In: Ztürk MÖ
et al (eds) Sabkha ecosystems, tasks for vegetation science, vol 46. Springer, Berlin, pp 95–104
Aboul-Enein AM, Abu El-Ela F, Shalaby AE, El-Shemy HA (2012) Traditional medicinal plants
research in Egypt: studies of antioxidant and anticancer activities. J Med Plant Res
6:689–703
Ahmad VU, Ghazala A, Uddin S, Ali MS (1993) Saponins from Zygophyllum propinquum.
Ait El Cadi M, Khabbal Y, Alaoui K, Faouzi M-A, Bruno E, Mahraoui L, Cherrah Y (2008) Activité
anti-diarrhéique de Zygophyllum gaetulum. Phytothérapie 6:2–4
Ait El Cadi M, Makram S, Ansar M, Khabbal Y, Alaoui K, Cherrah Y, Taoufik J (2012) Activité
anti-inflammatoire des extraits aqueux et éthanolique de Zygophyllum gaetulum. Ann Pharm Fr
70:113–116
Amin E, El-Hawary SS, Fathy MM, Mohammed RA, Zulfiqar TN, Wedge DE, Becnel JJ, Khan IA
(2011) Triterpenoidal saponins: bioactive secondary metabolites from Zygophyllum coc-
cineum. Planta Med 77:488–491
Aquino R, Tortora S, Fkih-Tetouani S, Capasso A (2001) Saponins from the roots of Zygophyllum
gaetulum and their effects on electrically-stimulated guinea-pigileum. Phytochemistry
56:393–398
Atta AH, Mouneir SM (2004) Antidiarrhoeal activity of some Egyptian medicinal plant extracts.
Ayad R, Rahai M, Azouzi S, Louaar S, Dendougui H, Akkal S, Medjroubi K (2012) Phytochemical
investigation of the endemic plant Zygophyllum cornutum. Chem Nat Compd 48:313–314
Bellakhdar J (1997) La pharmacopéee marocaine traditionnelle: médecine arabe ancienne et
savoirs populaires. Ibis Press, Paris, p 764
Bellakhdar J, Claisse R, Fleurentin J, Younos C (1991) Repertory of standard herbal drugs in the
Moroccan pharmacopoeia. J Ethnopharmacol 35:123–143
Boubaker J, Skandrani I, Bouhlel I, Ben Sghaier M, Neffati A, Ghedira K, Chekir-Ghedira L
(2010) Mutagenic, antimutagenic and antioxidant potency of leaf extracts from Nitraria retusa.
Food Chem Toxicol 48:2283–2290
Boubaker J, Bhouri W, Ben Sghaier M, Bouhlel I, Skandrani I, Ghedira K, Chekir-Ghedira L
(2011) Leaf extracts from Nitraria retusa promote cell population growth of human cancer
cells by inducing apoptosis. Cancer Cell Int 11:37
Chaâbane M, Maktouf S, Sayari N, Zouari S, Zeghal N, Ellouze-Ghorbel R (2014) Antioxidant

and antimicrobial properties of the extracts from Nitraria retusa fruits and their applications to
meat product preservation. Ind Crop Prod 55:295–303
Chaieb M, Boukhris M (1998) Flore succincte et illustrée des zones arides et sahariennes de
Tunisie. Associations pour la protection de la nature et de l’environnement, Sfax
Daoud HS, Al-Rawi A (1985) The flora of Kuwait: dicotyledoneae, vol 1. KPI Publishers, London
El Ghoul J, Ghanem-Boughanmi N, Ben-Attia M (2011) Biochemical study on the protective
effect of ethanolic extract of Zygophyllum album on streptozotocin-induced oxidative stress
and toxicity in mice. Biomed Prev Nutr 1:79–83
El Ghoul J, Smiri M, Ghrab S, Boughattas NA, Ben-Attia M (2012) Antihyperglycemic, antihyper-
lipidemic and antioxidant activities of traditional aqueous extract of Zygophyllum album in
streptozotocin diabetic mice. Pathophysiology 19:35–42
Hadj Salem J, Chevalot I, Harscoat-Schiavo C, Paris C, Fick M, Humeau C (2011) Biological
activities of flavonoids from Nitraria retusa (Forssk.) Asch. and their acylated derivatives. Food
Chem 124:486–494
Halim Ahmed F, Saad Hassan-elrady A, Hashish Nadia E (1995) Flavonol glycosides from Nitraria
retusa. Phytochemistry 40(349):351
Hani AME, Shaker KH, Pollmann K, Seifert K (1995) Triterpenoid saponins from Zygophyllum
species. Phytochemistry 4:1233–1236
Hassanean HA, Desoky EK (1992) An acylated isorhamnetin glucoside from zygophyllum sim-
plex. Phytochemistry. 31(9):3293–3294
Hassanean HA, El-Shanawany MA, Bishay DW, Franz G (1989) Saponins from Zygophyllum
album. Bull Pharmacol Sci 12:117
Hussein SR, Salwa Æ, Kawashty A, Mohamed Æ, Tantawy E, Nabiel Æ, Saleh AM (2009)
Chemosystematic studies of Nitraria retusa and selected taxa of Zygophyllaceae in Egypt.
Plant Syst Evol 277:251–264
Hussein SR, Mona MM, Lamyaa FI, Salwa AK, Nabiel AMS (2011) Flavonoids of Zygophyllum
album L. and Zygophyllum simplex L. (Zygophyllaceae). Biochem Syst Ecol 39:778–780
Jaouhari JT, Lazrek HB, Seddik AJ (1999) Hypoglycaemic response to Zygophyllum gaetulum
extracts in patients with non-insulin-dependent diabetes mellitus. J Ethnopharmacol
64:211–217
Ksouri R, Ksouri WM, Jallali I, Debez A, Magne C, Isoda H, Abdelly C (2012) Medicinal halo-
phytes: potent source of health promoting biomolecules with medical, nutraceutical and food
applications. Crit Rev Biotechnol 32:289–326
Le Houérou HN (1980) Browse in North Africa. In: Le Houérou HN (ed) Browse in Africa.
International Livestock Center for Africa, Addis Ababa, pp 55–82
Mahmoud T, Gairola S (2013) Traditional knowledge and use of medicinal plants in the Eastern
Desert of Egypt: a case study from Wadi El-Gemal National Park. J Med Plants Stud 1:10–17
Megdiche Ksouri W, Medini F, Mkadmini K, Legault J, Magné C, Abdelly C, Ksouri R (2013)
LC–ESI-TOF–MS identification of bioactive secondary metabolites involved in the antioxi-
dant, anti-inflammatory and anticancer activities of the edible halophyte Zygophyllum album
Desf. Food Chem 139:1073–1080
Mnafgui K, Hamden K, Salah H, Kchaou M, Nasri M, Slama S, Derbali F, Allouche N, Elfeki A
(2012) Inhibitory activities of Zygophyllum album: a natural weight-lowering plant on key
enzymes in high-fat diet-fed rats. Evid Based Complement Altern Med ID 620384:9
Mohamed AA, Ali SI, El-Baz FK, Hussein SR (2014) Comparative study of antioxidant activities
of Nitraria retusa and quantification of its bioactive components by GC/MS. Int J Pharm Sci
Rev Res 29:241–246
Pöllmanns K, Agel T, Elgamal HA, Shaker CA, Ifert K (1997) Triterpenoid saponins from the roots
of Zygophyllum species. Phvtochemistry 44:485–489
Saleh NAM, El-Hadidi MN (1977) An approach to the chemosystematics of the zygophyllaceae.
Biochem Syst Ecol 5:121–128
Subhashini P, Dilipan E, Thangaradjou T, Papenbrock J (2013) Bioactive natural products from

marine angiosperms: abundance and functions. Nat Prod Bioprospect 3:129–136
Tackholm V (1974) Student flora of Egypt. Published by Cairo University printed by cooperative
printing company
Tigrine-Kordjani N, Meklati BY, Chemat F (2006) Analysis by gas chromatography-mass spec-
trometry of the essential oil of Zygophyllum album L., an aromatic and medicinal plant growing
in Algeria. Int J Aromather 16:187–191
Tigrine-Kordjani N, Meklati BY, Chemat F (2011) Contribution of microwave accelerated distilla-
tion in the extraction of the essential oil of Zygophyllum album L. Phytochem Anal 22(1):1–9
White F (1983) The vegetation of Africa, a descriptive memoir to accompany the UNESCO/
AETFAT/UNSO vegetation map of Africa. UNESCO. Nat Resour Res 20:1–356
Zar Kalai F, Han J, Ksouri R, El Omri A, Abdelly C, Isoda H (2013) Antiobesity effects of an
edible halophyte Nitraria retusa Forssk in 3T3-L1 preadipocyte differentiation and in
C57B6J/L mice fed a high fat diet-induced obesity. Evid Based Complement Altern Med. ID
368658:11
Zar Kalai F, Han J, Ksouri R, Abdelly C, Isoda H (2014) Oral administration of Nitraria retusa
ethanolic extract enhances hepatic lipid metabolism in db/db mice model ‘BKS.Cg-Dock7m+/+
Leprdb/J’ through the modulation of lipogenesis–lipolysis balance. Food Chem Toxicol
72:247–256
In vivo Anticandida Activity of Three
Traditionally Used Medicinal Plants
in East Africa
D.P. Kisangau, K.M. Hosea, H.V.M. Lyaruu, C.C. Josep, Z.H. Mbwambo,

and P.J. Masimba
Abstract Crude extracts of Dracaena steudneri bark (DSB), Sapium ellipticum

bark (SEB) and Capparis erythrocarpos root (CER) were investigated for their anti-
fungal activity in immunocompromised mice infected with Candida albicans in an
in vivo mice infection model. Extracts of these plant species are commonly used to
treat fungal infections in East African countries. Three groups of white albino mice
were immunosuppressed with 200 mg/kg body weight of cyclophosmamide for four
consecutive days after which they were administered with 0.3 ml of 0.5 McFarland
standard inoculum of C. albicans. The groups were treated with escalating doses of
100, 200 and 400 mg/kg of body weight of dichloromethane extracts. There was
substantial dose dependency in all treatments given, with mice survival to the end of
the experiment correlating well to the dose levels. At a dose of 400 mg/kg, C. eryth-
rocarpos was the most effective with mice survival of 60% and organ burden clear-
ance ranging from 64.0%–99.9% (P < 0.0001) in all treatments. The results revealed
that C. erythrocarpos possessed significant potential for development into antifun-
gal drugs suitable for control of Candida infections. The effectiveness of the plant
extracts in vivo was a confirmation of the value of ethnopharmacological leads in
drug discovery.
D.P. Kisangau (*)

Department of Biology, South Eastern Kenya University, P.O Box 170-90300, Kitui, Kenya
K.M. Hosea
Department of Molecular Biology and Biotechnology, University of Dar es Salaam,
Dar es Salaam, Tanzania
H.V.M. Lyaruu
Department of Botany, University of Dar es Salaam, Dar es Salaam, Tanzania
C.C. Josep
Department of Chemistry, University of Dar es Salaam, Dar es Salaam, Tanzania
Z.H. Mbwambo • P.J. Masimba
Institute of Traditional Medicine, Muhimbili University of Health and Allied Sciences,
Dar es Salaam, Tanzania

DOI 10.1007/978-94-024-1120-1_14
358 D.P. Kisangau et al.
Keywords Antifungal • Medicinal plants • Ethnopharmacology • Dracaena steudneri

• Capparis erythrocarpos • Candida albicans
1 Introduction
Opportunistic fungal infections represent a significant cause of morbidity and mor-

tality in immunocompromised patients especially those living with HIV/AIDS
(Masoko and Eloff 2005; Scorzoni et al. 2007; Rekha and Vidyasagar 2013). Despite
the increase in fungal infections, therapeutic options are very limited as almost all
the antifungal agents currently in use have toxic side effects and are relatively
expensive (Diwan 2006; Ara et al. 2009). Virtually, all synthetic antifungals are
associated with serious adverse effects and for some fungal infections, there is still
no effective cure. Most common fungal infections are frequently difficult to eradi-
cate with topical preparations and may require long-term use of systemic drugs (De
Toledo et al. 2011; Ernst 2001). It is therefore clear that new antifungal agents with
broad spectrum fungicidal activities are needed for effective management of these
infections. Hence, there is a need for renewed studies on plant natural products that
could provide an option for discovery of effective, safer and less toxic antifungal
agents.
The aim of the present paper was therefore to investigate in vivo antifungal effi-
cacy of extracts of selected plant species used in traditional medicine in East African
countries.
2 Materials and Methods
Test Animals
Normal white albino mice aged between 5 and 6 months and weighing between 20
and 30 g were locally purchased from Vocational Education and Training Authority
(VETA), Dar es Salaam, and reared in the animal house facility of the Institute of
Traditional Medicine, Muhimbili University of Health and Applied Sciences
(MUHAS). The experimental mice were maintained in standard laboratory condi-
tions at ambient temperature with free access to food and water according to Diwan
(2006). The experimental animals were sourced and handled in accordance to
Tanzanian animal ethics and regulatory guidelines.
In vivo Anticandida Activity of Three Traditionally Used Medicinal… 359
Test Plant Extracts and Microorganism
Crude dicholoromethane extracts of C. erythrocarpos root (CER) D. steudneri bark

(DSB) and S. ellipticum bark (SEB) were chosen for the in vivo efficacy studies
based on their significant in vitro anticandida activity both as crude and semi-
purified fractions reported in a previous study (Kisangau et al. 2009). During treat-
ment, the dried extracts were dissolved in normal saline with 20% ethanol (v/v) in
order to avoid mice mortality due to solvent toxicity. The test organism used in this
study was C. albicans (ATCC 90028).
Inoculum and Immunosuppression Dose Studies
Preliminary experiments were carried out to determine the inoculum size and
immunosuppression dose sufficient to cause death of at least 90% or more of
untreated mice (Clark et al. 1991; Dogan et al. 2007; Warn et al. 2000; Rekha and
Vidyasagar 2013). The 90% lethal dose (LD90) was defined as the inoculum size of
the test organism which caused 90% mortality of untreated mice 10 days post
infection.
Treatment
Mice were randomized into three groups of 10 in separate cages. The three groups
were immunosuppressed with 200 mg/kg body weight of cyclophosmamide for four
consecutive days after which they were administered with 0.3 ml of 0.5 McFarland
standard inoculum of C. albicans. The immunosuppression dose of 200 mg/kg and
inoculum size of 0.3 ml of 0.5 McFarland standard were found to be the most suf-
ficient in causing up to 90% mice mortality (LD90) within 10 days of the experiment.
Twenty four hours post infection, one group was orally treated with escalating doses
of 100, 200 and 400 mg/kg of body weight of dichloromethane extracts using a
gavage needle. The second group, taken as positive control was intraperitoneally
treated with fluconazole (Pfzer Ltd. S. Africa) at 10 mg/kg body weight. Treatment
of the two groups was given twice daily for six consecutive days. The third group,
taken as negative control was left untreated for six consecutive days post infection.
Efficacy Assessment
On day 11 post infection, all surviving mice were sacrificed. The lungs, liver, brain
and kidneys were removed and transferred to 2 ml of sterile phosphate buffered
saline (PBS). The organs were homogenized in a tissue grinder and then diluted to
10−1, 10−2 and 10−3. A volume of 100 μl of each of the diluted suspensions was
transferred to Sabouraud dextrose agar plates and uniformly spread over the agar
surface. The plates were incubated at 37 °C and examined daily for 2 days. Colony
counts were recorded from all plates that showed growth. Treatments were termed
effective when geometric colony count was less than 3.7 × 105 c.f.u/ml per organ
homogenate, equivalent to 63.0% organ burden clearance12. Percentage organ bur-
den clearance in infected mice was calculated on the assumption that the initial 0.5
McFarland inoculum injected to the mice had 106 c.f.u/ml judged from the inocu-
lum turbidity.
Data Analysis
Kruskal-Wallis test was performed using GraphPad Instat® software Version 3.0 to
determine differences in organ burden clearance in the treatments.
3 Results
Survival Curves of Mice in Treatment Experiments
There was 100% survival of mice in all treatment groups at 100 mg/kg, 200 mg/kg
and 400 mg/kg of D. steudneri, S. ellipticum and C. erythrocarpos extracts one day
post infection. However, mice in all groups appeared very sick with a profound
morbidity within 24 h of infection. They became motionless, with bristled hair and
difficult breathing. After this initial period of morbidity, the condition of the mice in
some treated groups gradually stabilized to the end of the experiment as shown in
the survivorship curves in Figs. 1, 2 and 3. In the D. steudneri group, all untreated
mice (control) died 4 days post infection while all those in the 100 mg/kg treatment
dose died 5 days post infection (Fig. 1). Mice survival in the 200 mg/kg, 400 mg/kg
and fluconazole doses was reduced to 10%, 30% and 60% respectively by the end
of the 10 days post infection.
In S. ellipticum group, all untreated mice (control) and those treated with 100 mg/
kg dose died 4 days post infection (Fig. 2). Mice survival in the 200 mg/kg dose was
120
100
80
% survival
60
40
20
0
1 2 3 4 5 6 7 8 9 10
Days post infection
Fig. 1 Percentage survival of mice treated with D. steudneri stem bark extract
Key: ▲ 400 mg/kg, ■ 200 mg/kg, ♦ 100 mg/kg, ◊ Fluconazole (10 mg/kg), × Untreated
120
100
80
% survival
60
40
20
0
1 2 3 4 5 6 7 8 9 10
Days post infection
Fig. 2 Percentage survival of mice treated with S. ellipticum stem bark extract
reduced to 10% by the end of the 10 days post infection while in 400 mg/kg dose, it
was reduced to 20%. In the fluconazole dose (10 mg/kg), there was 60% mice sur-
vival by 10 days post infection.
In the C. erythrocarpos treatment group (Fig. 3), mice survival in both 100 mg/
kg and 200 mg/kg doses was reduced to 20% by 10 days post infection. Mice sur-
vival in 400 mg/kg was very closely comparable to that in fluconazole dose with
60% and 70% respectively. However, all untreated mice (control) died 8 days post
infection.
120
100
80
% survival
60
40
20
0
1 2 3 4 5 6 7 8 9 10
Days post infection
Fig. 3 Percentage survival of mice treated with C. erythrocarpos root extract

26.5
26
25.5
Mean weight (g)
25
24.5
24
23.5
1 2 3 4
Days pre-infection
Fig. 4 Mean weight of mice pre-infection (during immunosuppression) in D. steudneri treatment

group
Mean Weights of Mice Pre- and Post-infection
Mean weights of mice in all treatment groups were recorded for the first 10 days of
the experiment. However, mean weights of mice in the untreated groups were not
recorded as they were assumed to degenerate with subsequential death as observed
in the treatment experiments. The mean weight of mice in the 4 days of immunosup-
pression for D. steudneri, S. ellipticum and C. erythrocarpos treatment groups are
30
25
Mean weight (g)
20
100 mg/kg
15 200 mg/kg
400 mg/kg
10
Flucon (10
mg/kg)
5
0
1 2 3 4 5 6
Days post infection
Fig. 5 Mean weight of mice post-infection (during treatment) in D. steudneri treatment group
27.5
27
26.5
Mean weight (g)
26
25.5
25
24.5
24
1 2 3 4
Days pre-infection
Fig. 6 Mean weight of mice pre-infection (during immunosuppression) in S. ellipticum treatment

group
shown in Figs. 4, 6 and 8 while the mean weight of mice post infection are shown
in Figs. 5, 7 and 9. The pre-infection mean weight for D. steudneri treatment group
ranged from 25.0 g to 25.5 g and the grand mean weight was 25.4 g for this treat-
ment group. The pre-infection mean weight for S. ellipticum treatment group ranged
from 25.9 g to 26.5 g with a grand mean of 26.0 g. The pre-infection mean weight
for C. erythrocarpos treatment group ranged from 22.0 g to 22.4 g with a grand
mean of 22.2 g.
30
25
20
Mean weight (g)
100 mg/kg
200 mg/kg
15
400 mg/kg
10
Flucon (10
5 mg/kg)
0
1 2 3 4 5 6
Days post infection
Fig. 7 Mean weight of mice post-infection (during treatment) in S. ellipticum treatment group
23
22.8
22.6
22.4
Mean weight (g)
22.2
22
21.8
21.6
21.4
21.2
21
20.8
1 2 3 4
Days pre-infection
Fig. 8 Mean weight of mice pre-infection (during immunosuppression) in C. erythrocarpos treat-

ment group
In all the three treatment groups, there was a steady decline in mean weights of
mice immediately after immunosuppression challenge and following infection with
C. albicans. In the D. steudneri treatment group, the mean weight of mice in the
100 mg/kg dose declined up to 23.0 g on day four post infection, after which all
mice in this treatment dose died (Fig. 5). Similarly, there was a steady decline in
mean weight in the 200 mg/kg dose from 24.8 g on day one post infection to 22.5 g
on day six post infection. In the 400 mg/kg dose, there was a mean weight decline
of up to 21.0 g on day four post infection followed by a slight mean weight
improvement up to 23.0 g on day six post infection. Mean weight in the fluconazole
30
25
Mean weight (g)
20
100 mg/kg
15 200 mg/kg
400 mg/kg
10
Flucon (10
mg/kg)
5
0
1 2 3 4 5 6
Days post infection
Fig. 9 Mean weight of mice post-infection (during treatment) in C. erythrocarpos group
dose went down to 23.0 g day two post infection followed by a slight improvement
up to 24.0 g on day six post infection.
In S. ellipticum treatment group, there was no mean weight improvement in
100 mg/kg and 200 mg/kg doses. There was however a slight mean weight improve-
ment in the 400 mg/kg and fluconazole doses. In 100 mg/kg dose of C. erythrocar-
pos treatment group, mean weight declined to 18.4 g on day four post infection
followed by a slight improvement up to 19.4 g on day six post infection. A similar
pattern was observed in 200 mg/kg, 400 mg/kg and fluconazole doses (Fig. 9).
Organ Culture Counts of Mice in Autopsy
Geometric colony counts of the brain, kidneys, liver and lungs are shown in Tables
1, 2 and 3. The geometric colony counts were determined only where distinct col-
ony counts could be visually established in all the three dilutions (10−1, 10−2, 10−3).
However, for statistical purposes, treatments with countless colonies were calcu-
lated on the basis of 1000 colony counts as the minimum in the 10−3 dilution. In the
200 mg/kg dose of D. steudneri treatment group (Table 1), organ burden clearance
was more effective in the kidneys than in the other organs with an average colony
count of 9.3 × 103 c.f.u/ml, equivalent to 99.0% clearance. The liver was the most
burdened as colony counts were well above 1000 in 10−1 and 10−2 dilutions. Also, in
the 400 mg/kg dose, organ burden clearance was most effective in the kidneys with
an average colony count of 3.3 × 101 c.f.u/ml (99.9% clearance), while the liver was
the most burdened with colony counts above 1000. In the fluconazole group, organ
burden clearance was most effective in the lungs with colony counts of 1.7 × 102
c.f.u/ml (99.8% clearance). In the overall D. steudneri treatment group, organ
Table 1 Organ culture counts for C. albicans in D. steudneri stem bark extract treatment
c.f.u counta
Treatment Dilution Brain Kidneys Liver Lungs
100 mg/kgb 10−1 – – – –
10−2 – – – –
10−3 – – – –
c.f.u average count – – – –
200 mg/kg 10−1 9.0 × 104 2.9 × 103 >1000 3.0 × 105
10−2 3.5 × 104 2.5 × 104 >1000 8.0 × 102
10−3 1.0 × 104 0 1.4 × 105 3.0 × 104
c.f.u average count 4.5 × 104 9.3 × 103 – 1.1 × 105
(95.5%) (99.1%) (89.0%)
400 mg/kg 10−1 3.6 × 103 1.0 × 102 2.0 × 105 2.0 × 102
10−2 0 0 2.3 × 105 2.0 × 103
10−3 0 0 3.5 × 106 1.0 × 104
c.f.u average count 1.3 × 103 3.3 × 101 1.3 × 106 4.1 × 103
(99.8%) (99.9%) (−30.0%) (99.6%)
Fluconazole 10−1 1.2 × 105 3.5 × 105 1.8 × 103 5.0 × 102
(10 mg/kg)
10−2 5.5 × 104 0 1.0 × 103 0
10−3 0 0 0 0
(94.2%) (88.0%) (99.9%) (99.9%)
a
All counts are expressed as c.f.u/ml of organ homogenate and percentage organ burden clearance
is given in parenthesis for average colony counts only
b
No data for 100 mg/kg dose as all mice died before the end of the experiment
burden clearance was therefore most effective in the 400 mg/kg dose in kidneys
with average colony counts of 3.3 × 101 c.f.u/ml slightly higher than that of flucon-
azole at 1.7 × 102 c.f.u/ml in the lungs. There were significant differences in organ
burden clearance in the three D. steudneri treatment dosages (200 mg/kg. 400 mg/
kg and fluconazole) (P < 0.05).
In the S. ellipticum treatment group (Table 2), the lowest organ colony load was
observed in 200 mg/kg with an average colony count of 3.4 × 104 c.f.u/ml (96.6%
clearance) in the lungs. This was comparably lower than the minimum colony load
of 2.2 × 105 c.f.u/ml (78.0% clearance) and 7.5 × 104 c.f.u/ml (92.5% clearance)
observed in 400 mg/ml and fluconazole doses respectively. There were no significant
differences in organ burden clearance in the three S. ellipticum treatment dosages
(200 mg/kg. 400 mg/kg and fluconazole) (P > 0.05).
In the C. erythrocarpos treatment group (Table 3), percentage organ clearance in
the 400 mg/kg dose was well comparable to that of the fluconazole in the brain,
kidney and lungs. The lowest organ colony load was observed in the 400 mg/kg
dose with an average colony count of 3.3 × 101 c.f.u/ml (99.9% clearance) in the
kidneys. It was equivalent to that observed for fluconazole in the brain. The lowest
colony loads in 100 mg/kg and 200 mg/kg doses were 2.0 × 105 c.f.u/ml (80.0%
Table 2 Organ culture counts for C. albicans in S. ellipticum stem bark extract treatment.
c.f.u counta
100 mg/kgb 10−1 – – – –
10−2 – – – –
10−3 – – – –
c.f.u average count – – – –
200 mg/kg 10−1 >1000 8.0 × 104 7.2 × 104 4.5 × 104
10−2 3.8 × 105 2.1 × 104 2.2 × 105 3.2 × 105
10−3 7.5 × 105 0 1.2 × 106 1.1 × 106
c.f.u average count – 3.4 × 104 5.0 × 105 4.8 × 105
(96.6%) (50.0%) (52.0%)
400 mg/kg 10−1 5.6 × 104 4.8 × 104 1.0 × 105 7.2 × 104
10−2 4.0 × 105 4.0 × 106 6.8 × 104 5.2 × 105
10−3 2.1 × 105 1.2 × 106 2.8 × 106 7.7 × 105
(78.0%) (45.0%) (1.0%) (55.0%)
Fluconazole 10−1 1.0 × 105 >1000 1.0 × 105 2.0 × 105
(10 mg/kg) 10−2 9.5 × 104 >1000 4.4 × 105 1.0 × 106
10−3 3.0 × 104 >1000 2.0 × 104 4.0 × 105
c.f.u average count 7.5 × 104 – 1.9 × 105 5.3 × 105
(92.5%) (81.0%) (47.0%)
a
b
No data for 100 mg/kg dose as all mice died before the end of the experiment
clearance), and 3.2 × 104 c.f.u/ml (96.8% clearance) in kidneys and lungs respec-
tively. There were significant differences in organ burden clearance in the four C.
erythrocarpos treatment dosages (100 mg/kg, 200 mg/kg. 400 mg/kg and flucon-
azole) [P < 0.0001].
Based on the organ burden clearance cut point of 3.7 × 105 c.f.u/ml (63.0% clear-
ance), D. steudneri group had six effective treatments out of a total of 12 treatments
administered to the mice, with percentage organ burden clearance ranging from
89.0% to 99.9% (Table 1). S. ellipticum extract had two effective treatments with
percentage clearance ranging from 78.0% to 96.6% (Table 2). C. erythrocarpos
group had six effective treatments ranging from 64.0% to 99.9% (Table 3). Most of
these effective treatments of the plant extracts were quite comparable to the flucon-
azole treatments.
Percentage Organ Weights of Mice in Autopsy
The highest percentage organ weights of mice in autopsy were recorded for liver
and ranged from 5.4 to 7.5% (Table 4). Percentage organ weights for kidneys, lungs
and brain ranged from 1.5 to 2.4%, 1.0 to 1.6% and 1.5 to 2.1%, respectively.
Table 3 Organ culture counts for C. albicans in C. erythrocarpos root extract treatment
c.f.u counta
100 mg/kg 10−1 >1000 1.5 × 104 >1000 >1000
10−2 >1000 9.0 × 104 8.0 × 105 >1000
10−3 6.0 × 106 5.0 × 105 3.8 × 105 >1000
c.f.u average count – 2.0 × 105 – –
(80.0%)
200 mg/kg 10−1 >1000 8.0 × 104 9.8 × 104 8.0 × 104
10−2 >1000 5.4 × 105 8.0 × 105 1.5 × 104
10−3 3.6 × 106 9.0 × 105 1.9 × 105 0
c.f.u average count – 4.8 × 105 3.6 × 105 3.2 × 104
(52.0%) (64.0%) (96.8%)
400 mg/kg 10−1 6.0 × 103 1.0 × 102 >1000 1.3 × 103
10−2 0 0 4.5 × 105 0
10−3 0 0 7.1 × 105 0
c.f.u average count 2.0 × 103 3.3 × 101 – 4.3 × 102
(99.8%) (99.9%) (99.9%)
Fluconazole 10−1 1.0 × 102 3.0 × 102 9.0 × 103 4.8 × 104
(10 mg/kg) 10−2 0 1.0 × 103 5.0 × 103 3.4 × 104
10−3 0 0 1.0 × 104 7.0 × 104
(99.9%) (99.9%) (99.2%) (94.9%)
a
4 Discussion
Candida albicans remains the most common fungal pathogen causing deep mycosis
in humans (Marianne et al. 2000). Of all known opportunistic fungal infections, oral
candidiasis caused by C. albicans is one of the most common manifestations of
HIV/AIDS, and does not only influence the nutritional status of the HIV/AIDS
patients, but it is a predisposing factor to the other more serious disseminated infec-
tions (Rekha and Vidyasagar 2013; Runyoro et al. 2006; Moussa et al. 2012).
Among the different HIV-associated fungal infections, oral mucosal lesions caused
by Candida species are by far the most frequent manifestations. Candida albicans
is the most causative agent, accounting for more than 90% of cases (Blignaut 2007).
The fact that animals in all groups became very sick within 24 h of infection,
showing reduced mobility was a confirmation that they had reached a state of pro-
found neutropenia due to immunosuppression. This was also confirmed by the
100% mortality of all untreated mice during treatment experiments meaning that
they were unable to withstand Candida challenge due to the compromised immu-
nity. A state of immunosuppression was also confirmed by the sudden decline in
mean weights of mice 24 h post infection. In a related mice infection model,
Chinchilla et al. (1998), Warn et al. (2000) and Warn and Denning (1999) demon-
Table 4 Percentage organ Organ weight in

weight of mice in autopsy in Organ Treatment group autopsy (%)
different treatment groups
Liver DSB 200 mg/kg 7.1
DSB 400 mg/kg 6.4
Flucon-DSB 6.3
SEB 200 mg/kg 6.5
SEB 400 mg/kg 6.1
Flucon-SEB 6.9
CER 100 mg/kg 7.5
CER 200 mg/kg 5.5
CER 400 mg/kg 5.4
Flucon-CER 6.3
Kidney DSB 200 mg/kg 1.9
DSB 400 mg/kg 1.7
Flucon-DSB 2.4
SEB 200 mg/kg 1.7
SEB 400 mg/kg 1.7
Flucon-SEB 1.8
CER 100 mg/kg 1.9
CER 200 mg/kg 1.5
CER 400 mg/kg 1.9
Flucon-CER 1.6
Lungs DSB 200 mg/kg 1.6
DSB 400 mg/kg 1.3
Flucon-DSB 1.6
SEB 200 mg/kg 1.1
SEB 400 mg/kg 1.3
Flucon-SEB 1.3
CER 100 mg/kg 1.1
CER 200 mg/kg 1.1
CER 400 mg/kg 1.2
Flucon-CER 1.0
Brain DSB 200 mg/kg 2.0
DSB 400 mg/kg 1.9
Flucon-DSB 1.8
SEB 200 mg/kg 1.9
SEB 400 mg/kg 1.5
Flucon-SEB 2.1
CER 100 mg/kg 1.7
CER 200 mg/kg 1.7
CER 400 mg/kg 2.0
Flucon-CER 1.6
Flucon-DSB Fluconazole treatment in D. steudneri (DSB)
group, Flucon-SEB Fluconazole treatment in S. ellipticum
(SEB) group, Flucon-CER Fluconazole treatment in C.
erythrocarpos (CER) group
strated that no treatment group had 100% survival and that mice receiving no active
treatment had either 90% or 100% mortality.
The present model showed a substantial dose dependency in all the treatments
administered, with the mice survival to the end of the experiments correlating to the
dose levels as previously reported in a different study by Warn et al. (2000, 2005),
Diwan4 and You et al. (2009). In the C. erythrocarpos treatments, 400 mg/kg dose
which compared quite well with fluconazole in the survivorship curves was indica-
tive of the strong potential of this extract to eliminate Candida infections. The abil-
ity of the 400 mg/kg dose in the C. erythrocarpos treatment to clear colony burden
in the kidneys to 3.3 × 101 c.f.u/ml, lower than that of fluconazole (4.3 × 102 c.f.u/
ml) supports this credibility. Extract effectiveness was also portrayed in D. steud-
neri and S. ellipticum treatments as they were able to sustain a number of infected
mice to the end of the experiment. However, it remained uncertain as to why in C.
erythrocarpos treatments survival levels of mice in 100 mg/kg dose were higher
than in 200 mg/kg in days 4–6 post infection. It was also not clear why mice survival
in the C. erythrocarpos group was higher than in the D. steudneri group yet the two
had equal number (six) of effective treatments. Besides, D. steudneri treatments had
even a higher range of percentage organ clearance (89%–99.9%) than C. erythro-
carpos treatments with 64.0%–99.9%. But overall, organ burden clearance was
most effective in the D. steudneri and C. erythrocarpos treatments than in S. ellipti-
cum treatments. Elsewhere, the effect of an aqueous extract of Nigella sativa seeds
was studied on candidiasis in mice. An intravenous inoculum of Candida albicans
produced colonies of the organism in the liver, spleen and kidneys. Treatment of
mice with the plant extract produced a fivefold decrease of Candida in kidneys,
eightfold in liver and 11-fold in spleen (Ahmad et al. 2013).
The gradual improvement in mean weights of mice in the 400 mg/kg dose in all
treatment groups (D. steudneri, S. ellipticum and C. erythrocarpos) confirmed the
dose dependency treatment potentials of the plant extracts as previously reported by
Diwan (2006). A similar trend was observed in fluconazole treated mice. The grad-
ual gain in mean weights thus corresponded with the recovery of mice observed in
these treatments. This scenario substantiates the higher levels of mice survival to the
end of the experiment observed in the 400 mg/kg dose unlike in 100 mg/kg and
200 mg/kg doses where mice survival was limited. It may also explain why there
was rapid mortality in the untreated (control) groups as no mice survived by the end
of the experiment.
There was a clear correlation between mice survival and colony burden clearance
from organs in autopsy as previously reported in a different study by Masuoka
(2004) and You et al. (2009). Mice survival to the end of the experiment in 200 mg/
kg and 400 mg/kg doses of D. steudneri treatments could be associated with the
effective colony burden clearance observed in the brain, kidney and lungs. However,
mice survival in the 200 mg/kg and 400 mg/kg doses of S. ellipticum treatments
could only be associated with colony burden clearance in the kidneys and brain,
respectively. All other organs were burdened above the effective clearance level. In
the C. erythrocarpos treatment group, mice survival in the 100 mg/kg dose could be
ascribed to colony burden clearance in the kidneys while mice survival in the
200 mg/kg dose could be due to colony burden clearance in the liver and lungs. In
the 400 mg/kg dose, mice survival could be due to colony burden clearance in the
brain, kidneys and lungs. In a related study though on conventional antifungal, a
0.8 mg/kg dose of amphotericin B emulsion increased mice survival time compared
to untreated control group and was equally effective in reducing the c.f.u counts of
C. albicans in the kidney (Tabosa et al. 1996).
The highest percentage organ weights in autopsy were recorded in organs of
mice treated with lower doses than those treated with higher doses (Table 4). For
example the highest percentage organ weights for the liver were recorded in DSB
200 mg/kg (7.1%), SEB 200 mg/kg (6.5%) and CER 100 mg/kg (7.5%). A similar
pattern was observed for the kidneys, lungs and brain. This could be due to inflam-
mation of the organs caused by Candida infestation where colony burden clearance
was not very effective as similarly reported by Dogan et al. (2007). Uncertainly,
some organs of mice treated with the highest extract dose (400 mg/kg) or with flu-
conazole (10 mg/kg) registered high percentage organ weights. This was the case
for the kidneys in 400 mg/kg and fluconazole doses with percentage organ weight
of 1.9% and 2.4% respectively. It was also observed in lungs (Flucon-DSB, 1.6%)
and brain (CER 400 mg/kg, 2.0%).
5 Conclusion
This article presents the first antifungal activity assays of the three plant species (D.
steudneri, S. ellipticum and C. erythrocarpos) on animals. The results reveal that D.
steudneri and C. erythrocarpos possess significant potential for development into
antifungal drugs suitable for control of Candida infections. All the three plant spe-
cies are traditionally used in treatment of candidiasis and other fungal infections in
East African countries. Their effectiveness in vivo is a confirmation of the value of
ethnopharmacological leads in drug discovery. The study presents a platform for
pre-clinical trials of extracts of these plant species as potential candidates for devel-
oping therapies to manage Candida infections including HIV/AIDS opportunistic
infections associated with Candida spp. With the proven in vivo efficacy of the
extracts, standardized herbal formulations could be developed for mass production
in order to meet the health needs of many people who cannot afford conventional
medicines in developing countries.
Acknowledgments We are grateful to DAAD/NAPRECA, the Inter-University Council of East

Africa Research initiative (VicRes) and the International Foundation for Science (IFS) for financial
support to carry out this work. We thank Messrs. F.M. Mbago and S. Haji of the Herbarium, Botany
department, University of Dar es Salaam for identifying the plant species used in this study.
References
Ahmad A, Husain A, Mujeeb M, Khan A, Najmi AK, Siddique NA, Damanhouri ZA, Anwar F
(2013) A review on therapeutic potential of Nigella sativa: a miracle herb. Asian Pac J Trop
Biomed 3(5):337–352
Ara N, Nur MH, Amran MS, Wahid MII, Ahmed M (2009) In vitro antimicrobial and cytotoxic
activities of leaves and flower extracts from Lippia alba. Pak J Biol Sci 12(1):87–90
Blignaut E (2007) Oral candidiasis and oral yeast carriage among institutionalized South African
paedriatic HIV/AIDS patients. Mycopathologia 163:67–73
Chinchilla M, Guerrero OM, Abarca G, Barrios M, Castro O (1998) An in vivo model to study the
anti-malaric capacity of plant extracts. Int J Trop Biol 46:35–39
Clark JM, Whitney RR, Olsen SJ, George RJ, Swerdel MR, Kunselman L, Bonner DP (1991)
Amphotericin B Lipid Complex therapy of experimental fungal infections in mice. Antimicrob
Agents Chemother 35:615–621
De Toledo CEM, Britta EA, Ceole LF, Silva ER, De Mello JCP, Filh BPD (2011) Antimicrobial
and cytotoxic activities of medicinal plants of the Brazilian cerrado, using Brazilian cachac, as
an extractor liquid. J Ethnopharmacol 133(2):420–425
Diwan PV (2006) In vivo efficacy of an antifungal fraction from Pallavicinia lyellii, a liverwort.
Indian J Pharm 38(3):211–212
Dogan A, Ergen K, Budak F, Severcan F (2007) Evaluation of Disseminated Candidiasis on an
Experimental Animal Model: A Fourier Transform Infrared Study. Appl Spectrosc
61(2):199–203
Ernst E (2001) The desktop guide to complementary and alternative medicine: an evidence-based
approach. J Roy Soc Med 94:650–651
Kisangau DP, Hosea KM, Lyaruu HVM, Joseph CC, Mbwambo ZH, Masimba PJ, Gwandu CB,
Bruno LN, Devkota KP, Sewald N (2009) Screening of traditionally used Tanzanian medicinal
plants for antifungal activity. Int J Pharmaceut Biol 47(8):708–716
Marianne D, Ronald A, Froyen G, Frank CO, Fermin S, Roland C, Walter HMLL (2000) Single
allele knock-out of Candida albicans CGT1 leads to unexpected resistance to hygromycin B
and elevated temperature. Microbiologica 46:353–365
Masoko P, Eloff JN (2005) The diversity of antifungal compounds of six South African Terminalia
species (Combretaceae) determined by bioautography. Afr J Biotechnol 4(12):1425–1431
Masuoka J (2004) Surface Glycans of Candida albicans and other pathogenic fungi: physiological
roles, clinical uses, and experimental challenges. Clin Microbiol Rev 17(2):281–310
Moussa A, Noureddine D, Saad A, Abdelmalek A, Salima B (2012) The mixture of natural honey
and Peganum harmala seeds against Candida albicans by well diffusion method. Asian Pac
J Trop Biomed 6:1–4
Rekha S, Vidyasagar GM (2013) Anti-Candida activity of medicinal plants. A review. Int J Pharm
Pharm Sci 5(14):9–16
Runyoro DKB, Matee MIN, Ngassapa OD, Joseph CC, Mbwambo ZH (2006) Screening of
Tanzanian medicinal plants for anti-Candida activity. BMC Compl Altern Med 6:11
Scorzoni L, Benaducci T, Almeida AMF, Silva DHS, Bolzani VS, Gianinni MJSM (2007) The use
of standard methodology for determination of antifungal activity of natural products against
medical yeasts Candida sp and Cryptococcus sp. Braz J Microbiol 38:1–12
Tabosa Do Egito ES, Appel M, Fessi H, Barrett G, Puisieux F, Devissaguet J (1996) In-vitro and
in-vivo evaluation of a new amphotericin B emulsion-based delivery system. J Antimicrob
Chemother 38(3):485–497
Warn P, Denning DW (1999) Dose range evaluation of Liposomal Nystatin and comparisons with
Amphotericin B and Amphotericin B Lipid Complex in temporarily neutropenic mice infected
with an isolate of Aspergillus fumigatus with reduced susceptibility to Amphotericin
B. Antimicrob Agents Chemother 43(11):2592–2599
Warn PA, Morrissey J, Moore CB, Denning DW (2000) In-vivo activity of amphotericin B Lipid
complex in immunocompromised mice against Fluconazole-resistante or Fluconazole-
susceptible Candida tropicalis. Antimicrob Agents Chemother 44(10):2664–2671
Warn PA, Sharp A, Morrissey G, Denning DW (2005) Activity of aminocandin (IP960) compared
with amphotericin B and fluconazole in a neutropenic murine model of disseminated infection
caused by a fluconazole-resistant strain of Candida tropicalis. Antimicrob Agents Chemother
56:590–593
You X, Li C, Yang X, Yuan M, Zhang W, Lou R, Wang Y, Li G, Chen H, Song D, Sun C, Cen S, Yu
L, Zhao L, Jiang J (2009) In vivo antibacterial activity of vertilmicin, a new aminoglycoside
antibiotic. Antimicrob Agents Chemother 53(10):4525–4528
Wild Edible Allium Species from Arid Zones
of Tunisia: New Perspectives of Natural
Compounds
Hanen Najjaa, Abelkarim Ben Arfa, and Mohamed Neffati
Abstract Allium is the largest and the most important representative genus of the
Alliaceae family. For many centuries, several Allium species have been used as veg-
etables and spices, and as folk medicines for curing various diseases. Besides the
well-known garlic and onion, other species are either, widely cultivated for culinary
use, such as leek, scallion, shallot and chive, or found as wild endemic species
grown spontaneously.
This chapter presents an integrated view on new advances and recent trends in
the nutritional value, bioactive compound and health effect of various extracts pre-
pared from the spontaneous species, A. roseum (rosy garlic) and A. ampeloprasum
(Kurrath). Chemical composition of A. roseum leaves was investigated. The analy-
ses showed that A. roseum contained high amounts of soluble carbohydrate (32.8%),
crude protein (22.7%), dietary fibre (12.3%), ash (7.2%) and fat (3.6%). The most
abundant minerals were K, Ca and SO42− with 1530.5 and 712.5 mg/100 g DW,
respectively. Mn, Cu and Zn were also detected in appreciable amounts ranging
from 1 to 2 mg/100 g DW. Extracted leaf oil was composed of 14.93% saturated and
85.28% unsaturated fatty acids. Linolenic acid represents 53% of the unsaturated
fatty acids and palmitic acid represents 13% of the saturated fatty acids.
Phytochemical screening of A. roseum and A. ampeloprasum showed the presence
of bioactive agents exhibit a positive effect on human health as antioxidants and
antibacterial compounds such as saponins, tannins, flavonoids, coumarins, steroids,
cardiac glycosides, free quinone and iridoids. The most abundant phytonutrients in
A. roseum edible leaves included polyphenols, flavonoids, anthocyanidins, vitamin
C, carotenoids and allicin which were at 736.65 mg of equivalent catechol/100 g
DW, 3.37 mg of equivalent catechin/g DW, 1239.62 μg/100 g DW, 1523.35 mg/100
g DW, 242.25 μg/100 g DW and 657 mg/100 g DW, respectively. The content of
polyphenols, flavonoids and tannin in A. ampeloprasum was 29,278 mg of equiva-
lent gallic acid/g DW, 5.840 mg of equivalent quercetein/g DW and 5.509 mg of
equivalent catechin/g DW, respectively. The results indicate that leaves of A. roseum
H. Najjaa (*) • A. Ben Arfa • M. Neffati

Laboratoire d’Ecologie Pastorale, Institut des Régions Arides, 4119 Médenine, Tunisie

DOI 10.1007/978-94-024-1120-1_15
and A. ampeloprasum present a higher antioxidant capacity with a quenching DPPH

value equal to 3.78 and 30.12 mg Trolox/g DW respectively. The study revealed that
rosy garlic and kurrath are a rich source of many important nutrients and bioactive
compounds responsible for many promising health beneficial physiological effects
and they may be considered a functional food.
Keywords Allium roseum • A. ampeloprasum • Nutritional value • Bioactive com-

pounds • Antioxidant activity • Antibacterial activity • Functional food
1 Introduction
Tunisia occupies an area of about 164.103 km2. Three quarters of which are arid and
desert regions (Le Houerou 1959) characterized by low rainfall, high evapotranspi-
ration, high temperature and desiccating winds. Among 2250 species that compose
its vascular flora (Le Houerou 1959), 1630 species belong to the steppic zone (Le
Houerou 1959). Yet, a wide range of plants thrive in these ecosystems, which is of
high economical and ecological significance. Indeed, these plants have the ability to
tolerate the high temperature and drought and have a high capacity to synthesis and
accumulate large amount of bioactive substances against the abiotic stresses.
In plants, unfavourable environmental conditions such as drought and high tem-
perature increase production and accumulation of reactive oxygen species (ROS),
leading to cellular damage, metabolic disorders and senescence processes. Aromatic
and Medicinal Plant (AMP) of arid and desertic region are known for their ability to
withstand and quench these toxic ROS, since they are equipped with a powerful
antioxidant system (Khadri et al. 2010; Akrout et al. 2011; Najjaa et al. 2011b). For
economical and security reasons, the identification of new sources of natural anti-
oxidants is a promising alternative for their use in the food industry and in preven-
tive medicine to replace synthetic antioxidants (Tadhani et al. 2007). For that, there
is an increasing interest to identify among aromatic and medicinal plants (AMP) of
Arid and desertic species those with high antioxidant content for their use in the
agri-food industry and/or pharmaceutical and cosmetic applications.
AMP of arid and desertic regions are naturally drought-tolerant plants that con-
tain potential medicinal/nutraceutical properties and may be potentially useful as
new sources of functional compounds in dietary food. Interestingly, numerous of
tunisian’s AMP of arid and desertic land have been used in folklore medicines.
Recently, extracts of these plants have proven activity against human, animal, and
plant pathogens and more investigations have been carried out to identify the metab-
olites responsible for their bioactivities for interest to modern industry and medicine
(Najjaa et al. 2012; Chao et al. 2013).
As for as being beneficial to human health, Allium plants are already well known.
For example, garlic (A. sativum), is of particular interest owing to its prophylactic
and therapeutic actions. Antioxidative and antimicrobial activities of some Allium
species have been previously reported (Sharma and Prasad 2001; Banerjee and
Wild Edible Allium Species from Arid Zones of Tunisia: New Perspectives… 377
Maulik 2002; Najjaa et al. 2007, 2011a, b). This ability has mainly been attributed
to a variety of sulphur containing compounds, their precursors and other numerous
phenolic compounds (Kim et al. 1997; Benkeblia 2007).
In this chapter we interested specially on wild A. roseum (rosy garlic) and A.
ampeloprasum (kurrath) which are very polymorphous and widespread species
(Cuénod 1954; Le Floc’h 1983).
The fresh young leaves and bulbs of A. roseum and A. ampeloprasum were con-
sumed in salads and used as spice to prepare traditional recipes. Besides their culi-
nary use, rosy garlic and Kurrath are also used in folk medicine. Le Floc’h (1983)
reported that A. roseum is used use for the treatment of headaches and rheumatism.
It is also used for the treatment of bronchitis, colds as an inhalation, fever diminu-
tion and as an appetizer. The uses of this plant as functional food ingredient and/or
food supplement in the food industry and as base for the development of new drugs
in phytomedecine were also reported (Najjaa et al. 2007, 2011b).
The objective of the present study was to characterize nutritional properties, bio-
active components, and functional proprieties of A. roseum and A.ampeloprasum
grown in arid land of Tunisia and to infer their role in human nutrition.
2 Nutritional Value
The nutritional proprieties of A. roseum has recently determined by Najjaa et al.
(2012).
Compared to the carbohydrates content of aerial parts of other Alliums (5–12%)
(Brewster 1994), the leaves of A. roseum can be considered as rich sources of solu-
ble carbohydrates (32.80 g/100 g DW ± 0.21). The same other added that A. roseum
aerial part fibres content (12.30 g/100 g DW ± 0.05) was higher than that reported
for A. cepa bulb (1.7%), and proteins rate (22.70 g/100 g DW ± 1.51) was relatively
high compared to A. sativum bulbs (9.3%) and A. cepa (1.7%) (Haciseferoğullari
et al. 2005; Dini et al. 2008). Fats accounted for 0.68% of the fresh weight of A.
roseum, making them the least abundant class of nutrients.
A. roseum is also characterized by high ash content (7.20 g/100 g DW ± 1.31)
including macro and micro elements (Najjaa et al. 2012). The mineral element com-
position of A. roseum exhibited a higher concentration of potassium than calcium
and magnesium. The high content of potassium (1530.500 mg/100 g DW ± 0.036),
in A. roseum which is nutritionally significant, since it contributes to the control of
hypertension which results in excessive excretion of potassium (Dini et al. 2008).
Calcium is found at relatively high concentration in A. roseum (712.500 mg/100 g
DW). Onion leaf calcium concentration (2540 mg/100 g fresh weight) (Boukari
et al. 2001) is much higher than that of A. roseum leaves but bulb calcium concentra-
tion (45 mg/100 g fresh weight) (Adrian et al. 1995) is much lower. The low sodium
content of A. roseum and consequently low Na/K ratio (0.03) is another indication
that A. roseum consumption would reduce the incidence of hypertension (Iqbal et al.
2006). A. roseum leaves also contains several oligo-elements including iron, zinc,
copper and manganese (Najjaa et al. 2012).
Najjaa and their collaborator show that the fatty acid composition of A. roseum
leaves revealed twelve compounds. The unsaturated fatty acids were represented
mainly by linolenic, linoleic, oleic and gadoleic, and five saturated acids (palmitic,
stearic, myristic, arachidic and margaric), accounted for ~15% of the total fatty
acids (Najjaa et al. 2012).
3 Bioactives Substance
Phytochemical screening of A. roseum and A. ampeloprasum organic and aqueous

extracts using thin-layer chromatography (TLC), are reveals the presence of bioac-
tive compounds such as saponins, tannins, flavonoids, coumarins, steroids, cardiac
glycosides, free quinone and iridoids (Najjaa et al. 2011c). Such results may estab-
lish a good support to the use of this plant in herbal medicine and as base for the
development of new drugs and phytomedicine.
The content of potential health-promoting substances, flavonoid, total phenolic
and flavonoids content, vitamin C, tannin, anthocyanidin, carotenoids and allicin in
the wild A. roseum growing in the arid region of Tunisia were determined recently
by Najjaa et al. (2012). Total phenolic content of A. roseum are 736.65 ± 1.51 mg
CA/100 g DW and 3.37 mg CE/g DW respectively. Allium species are among the
richest sources of dietary flavonoids and contribute significantly to the overall intake
of flavonoids (Slimestad et al. 2007). In vitro and in vivo pharmacological tests have
shown that flavonoids exhibit the following variety of actions: (i) antioxidative
(Boyle et al. 2000); (ii) reduction of cardiovascular disease (Janssen et al. 1998) and
(iii) reduction of carcinogenic activity (Steiner 1997). The content of polyphenols,
flavonoids and tannin in A. ampeloprasum was 29.278 mg of equivalent gallic acid/g
DW, 5.840 mg of equivalent quercetein/g DW and 5.509 mg of equivalent catechin/g
DW, respectively.
The high A. roseum vitamin C content (1523.35 mg/100 g DW) may be an impor-
tant reason that it has been reputedly used as a traditional Tunisian medicine for
treating rheumatism and cold.
Garlic antibacterial bioactive principal was identified as diallylthiosulphinate
and was given allicin as trivial name since 1944. This bioactive substance is also
detected and with the same concentration in A. roseum (0.0328 μg/mL) (Najjaa
et al. 2012).
The global chromatographic analysis of A. roseum essential oil showed nineteen
compounds representing 91.4% of the whole oil, with a relatively high content of
organic sulphurous compounds representing about 26% of the total eluted com-
pounds in essential oil A. roseum (Najjaa et al. 2007). Methional presented the high-
est percentage (17.1%) among all the compounds of the essential oil of A. roseum.
As this compound is going to be mentioned by Najjaa et al. (2007) for the first time
in the essential oil of Allium genus with such relatively high level, methional used
as an important descriptor to characterize the essential oil of A. roseum and conse-

quently this endemic species itself (Najjaa et al. 2007). The S-containing com-
pounds are responsible for the appropriate smell and taste and also the health
benefits of members of Aliliaceae family.
Thiosulfonates which are very unstable would further decompose to produce a
series of degraded compounds including sulphides, according to the solvent used
and extraction conditions (Lanzotti 2006). For that raison, the analysed the thiosul-
fonate precursors in A. roseum and A. ampeloprasum using CLPH. The results show
that both studied species are characterized by the presence of the two -glutamyl
peptides γ-L-glutamyl-S-(allyl)-L-cysteine (GluAISC) and γ-L-glutamyl-S-(trans-
1-propenyl)-Lcysteine (GluPeCS). Their corresponding sulfoxide derivatives, are
(+)-S-methyl-L-cysteine sulfoxide (methiin), (+)-S-(trans-1-propenyl)-L-cysteine
sulfoxide (isoalliin) and (+)-S-(2-propenyl)-L-cysteine sulfoxide (alliin) in A
roseum and isoalliin and methiin in A. ampeloprasum. These differences in their
flavours can correlated with the vicinity of A. roseum with garlic and A. ampelopra-
sum with onion (Najjaa et al. 2011c).
The antioxidant activities of A. roseum leaf extracts were assessed and confirmed
using two functional analytical methods based on the radicals (ABTS and DPPH)
scavenging potential. The extracts obtained were all able to inhibit the DPPH, as
well as ABTS radicals. The antioxidant potential was 3.78 mg Trolox/g DW with
the DPPH method, and 3.99 mg Trolox/g DW with the ABTS (Najjaa et al. 2011b).
In comparison to previous data based on the ABTS scavenging capacity, A. roseum
leaf extracts were comparable or higher than other investigated species known to be
rich in antioxidants including strawberry (25.9), raspberry (18.5), red cabbage
(13.8), broccoli (6.5), and spinach (7.6) (Proteggente et al. 2002).
The assessment of antioxidant activity showed that A. ampeloprasum methanol
extract able to scavenge DPPH (30.12 mg Trolox/g DW) and has an important iron
(II) chelators with IC50 values of 6.03 mg/ml.
The important antioxidant activity of Allium species collected from arid land of
assessed by the different systems could be attributed to their high total polyphenolic
contents; in fact, it has been found that polyphenols are one of the most effective
antioxidative constituents in the plant (Velioglu et al. 1998). Moreover, the high
yield of the different phenolic compounds found in these plants might contribute to
the potent antioxidant activity of the extracts, since a positive correlation between
phenolic composition and antioxidant activity was proved (Que et al. 2006).
4 Health Effects and Future Trends
The importance of functional foods, nutraceuticals and other natural health products
has been well recognized in connection with health promotion, disease risk reduc-
tion and diminution in health care costs (Shahidi and Marian 2003). The expression
of nutraceutical was firstly mentioned 20 years ago to describe a union between
nutrition and pharmaceutics, both key contributors to human wellness. In the last
years, several research publications were devoted to so-called “functional food” and
“nutraceuticals” (Bernal et al. 2011). The boundary between nutraceutical and func-
tional food is not always clear, but the main difference is the format in which they
are consumed: nutraceuticals are consumed as capsules, pills, and tablets, whereas
functional foods are always consumed as ordinary foods. The results of the screen-
ing assays in these recent publications, indicating a synergistic of functional proper-
ties and composition of the extracts activities, justify the use of the investigated
plant in the Tunisian food and ethnomedicine. Thus, their possible use as natural
additives emerged from a growing tendency to replace synthetic substances by natu-
rally derived ones. From this point of view, our study may be considered as an
important report based on antioxidative potential of a wild Allium species growing
in the arid region of Tunisia and could be evaluated as a starting point for further
investigations with other species from their bioclimatic stage.
5 Conclusions
This study revealed that A. roseum gowning in Tunisia had a high soluble carbohy-
drates, crude protein and dietary fibre contents, comparatively to other Alliums. Its
mineral content was high in potassium, and calcium. The mineral composition of
‘rosy garlic’ is sufficient in Ca, P, K, Cu, Fe, Zn and Mg so that it can meet many
macronutrient and micronutrient requirements of the human diets. Furthermore,
edible part oil included 15% saturated and 85% unsaturated fatty acids. Linolenic
acid and palmitic acid were the most abundant unsaturated and saturated fatty acids,
respectively. This fatty composition confers to the A. roseum oil considerable nutri-
tional value, acting on physiological functions and reducing cardiovascular, cancer
and arthroscleroses diseases occurrence risk. The most abundant phytonutrients
found in A. roseum and A. ampeloprasum (polyphenolic compounds, flavonoids,
anthyacinidins, vitamin C and allicin) exhibit a positive effect on human health as
antioxidants compounds. Since A. roseum and A. ampeloprasum are a rich source of
many important nutrients and bioactive compounds responsible for many promising
health beneficial physiological effects, it may be considered a nutraceutical that
serves as a natural source of necessary components to help fulfil our daily nutri-
tional needs and as a functional food as well as in ethnomedecine.
A. roseum and A. ampeloprasum extracts exhibit interesting antioxidant proper-
ties correlated with their phytochemical composition, explaining, at least partially,
their usefulness in traditional medicine. The in use of these plants and their deriva-
tives for the primary purpose of flavouring and preserving foods will be of interest
for further study.
References
Adrian J, Potus J, Frangne R (1995) La science alimentaire de a à z. Lavoisier, 2ème Edition,

Paris, 457 p
Akrout A, Alarcon Gonzalez L, El Jani H, Campra Madrid P (2011) Antioxidant and antitumor
activities of Artemisia campestris and Thymelaea hirsuta from southern Tunisia. Food Chem
Toxicol 49:342–347
Banerjee SK, Maulik SK (2002) Effect of garlic cardiovascular disorders. Nutrition 1:1–14
Benkeblia N (2007) Antimicrobial activity of essential oil extracts of various onions (Allium cepa)
and garlic (Allium sativum). Lebensm-Wiss Technol 37:263–268
Bernal J, Mendiola JA, Ibanez E, Cifuentes A (2011) Advanced analysis of nutraceuticals. J Pharm
Biomed Anal 55(4):758–744
Boukari I, Shier NW, Xinia EFR, Frisch J, Bruce AW, Pawloski L, Fly AD (2001) Calcium analysis
of selected Western African foods. J Food Compos Anal 14:37–42
Boyle SP, Dobson VL, Duthie SJ, Kyle JAM, Collins AR (2000) Absorption and DNA protective
effects of flavonoid glycosides from onion meal. Eur J Nutr 39:213–223
Brewster JL (1994) Onions and other vegetable Alliums. CAB International, Wallingford
Chao HC, Najjaa H, Villareal OM, Ksouri R, Junkyu H, Neffati M, Hiroko I (2013) Arthrophytum
scoparium inhibits melanogenesis through the down-regulation of tyrosinase and melanogenic
gene expressions in B16 melanoma cells. Exp Dermatol 22:131–136
Cuénod A (1954) Flore analytique et synoptique de la Tunisie. Cryptogames vasculaires,
Gymnospermes et Monocotylédones. S.E.F.A.N, Tunis
Dini I, Tenore GC, Dini A (2008) Chemical composition, nutritional value and antioxidant proper-
ties of Allium caepa L. Var. tropeana (red onion) seeds. Food Chem 107:613–621
Haciseferoğullari H, Özcan M, Demir F, Çalişir S (2005) Some nutritional and technological prop-
erties of garlic (Allium sativum L.) J Food Eng 68:463–469
Iqbal A, Khalil IA, Ateeq N, Khan MS (2006) Nutritional quality of important food legumes. Food
Chem 97:331–335
Janssen K, Mensink RP, Cox FJ, Harryvan JL, Hovenier R, Hollman PC, Katan MB (1998) Effects
of the flavonoids quercetin and apigenin on hemostasis in healthy volunteers: results from an in
vitro and a dietary supplemented study. Am J Clin Nutr 67:255–262
Khadri A, Neffati M, Smiti S, Falé P, Lino ARL, Serralheiro MLM, Araújo EMM (2010)
Antioxidant, antiacetylcholinesterase and antimicrobial activities of Cymbopogon schoenan-
thus L. Spreng (lemon grass) from Tunisia. Food Sci Technol 43(2):331–336
Kim SM, Kubota K, Kabayashi A (1997) Antioxidative activity of sulphur-containing flavour com-
pounds in garlic. Biosci Biotechnol Biochem 61:1482–1485
Lanzotti V (2006) The analysis of onion and garlic. J Chromatogr A 1112:3–22
Le Floc’h E (1983) Contribution à une étude ethnobotanique de la flore tunisienne. Programme
flore et végétation tunisienne. Ministère de l’Enseignement Supérieur et de la Recherche
Scientifique, Tunis
Le Houerou HN (1959) Recherches écologiques et floristiques sur la végétation de la Tunisie méri-
dionale. Institut de Recherches sahariennes de l’Université d’Alger 2:510p
Najjaa H, Neffati M, Zouari S, Ammar E (2007) Essential oil composition and antibacterial activ-
ity of different extracts of a North African endemic species. C R Chim 10:820–826
Najjaa H, Zerria K, Fattouch S, Ammar E, Neffati M (2011a) Antioxidant and antimicrobial activi-
ties of Allium roseum “Lazoul”, a wild edible endemic species in North Africa. Int J Food Prop
14:371–380
Najjaa H, Zouari S, Ammar E, Neffati M (2011b) Phytochemical screening and antibacterial prop-
erties of Allium roseum L. a wild edible species in North Africa. J Food Biochem 35:699–714
Najjaa H, Zouari S, Ingrid A, Auger J, Ammar E, Neffati M (2011c) Différences et similitudes
des métabolites secondaires chez deux espèces du genre Allium, Allium roseum L. et Allium
ampeloprasum L. Acta Botanica Gallica 158(1):111–123
Najjaa H, Fattouch S, Ammar E, Neffati M (2012) Allium species, ancient health food for future.
Scient Health Soc Aspect Food Chem 17:343–354 (edition by Benamin Valdez)
Proteggente AR, Pannala AS, Pagana G, Van Buren L, Wagner E, Wiseman S (2002) The antioxi-
dant activity of regularly consumed fruit and vegetables reflects their phenolic and vitamin C
composition. Free Radic Res 36:217–233
Que F, Mao L, Pan X, (2006) Antioxidant activities of five Chinese rice wines and involvement of
phenolic compounds. Food Research International 581–587
Shahidi F, Marian N (2003) Phenolics in food and neutraceuticals, vol 1. CRC Press LLC, Boca
Raton, pp 144–150
Sharma GP, Prasad S (2001) Drying of garlic (Allium sativum L.) cloves by microwave-hotair
combination. J Food Eng 50:99–105
Slimestad R, Fossen T, Vagen IM (2007) Onions: a source of unique dietary flavonoids. J Agric
Food Chem 55:10067–10080
Steiner M (1997) The role of flavonoids and garlic in cancer prevention. In: Ohigashi H (ed) Food
Factors for Cancer Prevention. Springer, Tokyo, pp 222–225
Tadhani MB, Patel VH, Subhah R (2007) In vitro antioxidant activities of Stevia rebaudian leaves
and callus. J Food Compos Anal 20:323–329
Velioglu YS, Mazza G, Gao L, Oomah BD (1998) Antioxidant activity and total polyphenolics in
selected fruits, vegetables, and grain products. J Agric Food Chem 46:4113–4117
Utilization of Pteridophytes as Herbal
Medicines in Sub-Saharan Africa
Alfred Maroyi
Abstract Use of pteridophytes for medicinal purposes is entrenched in cultural

practices throughout sub-Saharan Africa. The current study is aimed at answering
the following questions through a study of the literature: (i) Which pteridophyte
species are used for medicinal purposes in sub-Saharan Africa? (ii) What are their
therapeutic uses, preparation and administration? and (iii) What are the geospatial
patterns of medicinal pteridophytes in sub-Saharan Africa? This investigation is
based on review of the literature published in scientific journals, books, conference
papers, floras, theses, reports from national, regional and international organizations
obtained from libraries and electronic search of Google Scholar, ISI Web of Science
and Scopus. A total of 99 pteridophyte species are used for medicinal purposes in
sub-Saharan Africa, corresponding to 8.4% of the total pteridophyte species of the
African continent. A total of 42 medical conditions are treated using herbal reme-
dies made from pteridophyte species. Ethnopharmacological studies are recom-
mended for the documented pteridophytes species as some of them have already
shown interesting applications in primary healthcare.
Keywords Pteridophytes • Medicinal • Cultural and ritualistic uses • Traditional

medicine • Sub-Saharan Africa
1 Introduction
Traditional medicines have been used to treat human and animal diseases in sub-
Saharan Africa for many generations before the introduction of orthodox medicine.
Use of traditional medicines in sub-Saharan Africa is widely practiced and this
practice is generally regarded as part of the African culture. Traditional medicines
therapy, although still an unwritten science, is well established in some cultures and
traditions (Shale et al. 1999), with the World Health Organization estimating that up
A. Maroyi (*)
Department of Botany, Faculty of Science and Agriculture, University of Fort Hare,
Private Bag X1314, Alice 5700, South Africa

DOI 10.1007/978-94-024-1120-1_16
384 A. Maroyi
Fig. 1 Young fronds of

Pteridium aquilinum (L.)
Kuhn developing after the
start of the rains
to 80% of the people in developing countries still depend on herbal medicines to

fulfil their primary healthcare needs (WHO 2002). About 25–50% of all current
pharmaceutical drugs are derived from plants (Harnett et al. 2005). Of the 252 drugs
considered as basic and essential by the World Health Organization (WHO), 11%
are exclusively of plant origin and a significant number are synthetic drugs obtained
from natural precursors (Rates 2001). It is not so much the case that traditional
medicines offer an alternative form of medical care, but rather it is in fact the only
source of treatment available for many people, particularly those living in undevel-
oped rural and marginalized areas in sub-Saharan Africa (WHO 2002).
The medicinal qualities of pteridophytes were recognized as early as 300 BC by
the Greek philosopher Theophrastus (Puri 1970). Pteridophytes existing today rep-
resent an ancient plant group which appeared about 300 million years ago in the late
Devonian period (Fernández et al. 2011). Pteridophyte species (Fig. 1), including
ferns and fern allies (Maroyi 2014a) play an important role as sources of traditional
medicines and other functions important for people’s livelihoods in the tropics and
subtropics. Isolated studies conducted in Lesotho (Moteetee and van Wyk 2011),
Nigeria (Nwosu 2002), South Africa and Swaziland (Roux 2003) showed that herbal
medicines derived from pteridophytes are used to cure many human and animal
diseases and disorders. Published literature on pteridophytes used in African tradi-
tional medicine usually lack details on therapeutic uses, preparation and administra-
tion of medicinal pteridophytes. A significant number of biologically active
compounds have been isolated from medicinal pteridophyte species in the past,
based on their medicinal applications and uses (Ho et al. 2010). Therefore, pterido-
Utilization of Pteridophytes as Herbal Medicines in Sub-Saharan Africa 385
phytes used as traditional medicines in sub-Saharan Africa may represent one of the
untapped sources of biologically active compounds that need to be exploited as
possible sources of pharmaceutical drugs. The current study is aimed at answering
the following questions through a study of the literature:
1. Which pteridophyte species are used for medicinal purposes in sub-Saharan
Africa?
2. What are their therapeutic uses, preparation and administration?
3. And finally, what are the geospatial patterns of medicinal pteridophytes in sub-
Saharan Africa?
2 Research Methods
Pteridophytes documented in literature as having medicinal uses in sub-Saharan

Africa are recorded in this study. Most of the available ethnobotanical information
on pteridophytes characterized by medicinal uses is scattered in various publica-
tions such as books, floras, scientific journals, theses, reports from natitional,
regional and international organizations. The total number of scientific papers,
books and other literature sources used in this study is 56. Literature was searched
on international online databases such as ISI Web of Science, Scopus and Google
Scholar using specific search terms such as “medicinal ferns or pteridophytes”.
References were also identified by searching the library collections of the University
of Fort Hare, South Africa. All plant scientific names, plant families and plant
authorities were verified using internet sources such as the International Plant Name
Index (www.ipni.org), the Missouri Botanical Garden’s Tropicos Nomenclatural
database (www.tropicos.org) and the Royal Botanic Garden and Missouri Botanic
Garden plant name database (www.theplantlist.org). For each species, data was also
collected from literature on countries in which the species are utilized, therapeutic
use(s), mode of administration and preparation of the species. Disease and ailment
categories were classified according to ailment categories proposed by Cook (1995).
3 Results and Discussion
Pteridophyte Diversity
A total of 99 pteridophyte species are used for medicinal purposes in sub-Saharan

Africa (Table 1), corresponding to 8.4% of the total pteridophyte species of the
African continent. About 627 pteridophyte species are known to grow in Africa and
an additional 557 species have been recorded in Madagascar (Aldasoro et al. 2004;
Kornaś 1993). These species belong to 24 families and 41 genera (Table 1). The
medicinal use category includes pteridophytespecies used for treating human and
386 A. Maroyi
Table 1 Pteridophytes used for medicinal purposes in sub-Saharan Africa

Species and family Country Use(s) Reference(s)
Acrostichum aureum L., Tanzania Tea made from dry leaves Matthews
Pteridaceae drunk as remedy for (1993)
epilepsy
Adiantum aethiopicum L., Nigeria Decoction of whole plant Nwosu (2002)
Adiantaceae used as tonic against
bruises, gangrenous
wounds, expectorant and
diuretic disorders
South Africa Leaves smoked for head van Wyk et al.
and chest colds (2013)
Adiantum capillus-veneris Lesotho Leaves smoked for head Moteetee and
L., Adiantaceae and chest colds van Wyk (2011)
Nigeria Leaf infusion drunk against Nwosu (2002)
infertility and vaginal
discharge; decoction of
roots used for washing hair
to remove dandruff;
powdered rhizome mixed
with camphor and dissolved
in palm-kernel oil applied
externally to hair infested
by lice
South Africa Tea made from the plant van Wyk et al.
and leaves smoked for head (2013)
and chest colds
Swaziland Leaves smoked for Roux (2003) and
respiratory tract disorders Long (2005)
and used for hair and scalp
problems
Adiantum caudatum L., Nigeria Decoction used for treating Nwosu (2002)
Adiantaceae jaundice in animals and
scabies; potherb taken
internally against
abdominal pain and
constipation
Adiantum incisum Forsk., Nigeria Leaf infusion used against Nwosu (2002)
Adiantaceae malaria and bronchial
diseases
Adiantum poiretii Wikstr., South Africa Leaves smoked for head Roux (2003)
Adiantaceae and chest colds
Swaziland Leaves smoked for Long (2005)
respiratory tract disorders
problems
(continued)
Table 1 (continued)
Adiantum raddianum Swaziland Leaves smoked for Long (2005)
C. Presl, Adiantaceae respiratory tract disorders
problems
Adiantum venustum Nigeria Extract of rhizome and Nwosu (2002)
G. Don, Adiantaceae leaves drunk against
diabetes, liver problems and
used as diuretic; infusion
taken internally for
circulatory disorders
Adiantum vogelii Mett. ex Gabon Leaves used as herbal Sassen and Wan
Keys, Adiantaceae medicine (2006)
Ivory coast Plant used for faints, Bouquet (1974)
treatment of debility, edema
and skin lesions of leprosy
Aleuriopteris Nigeria Decoction of whole plant Nwosu (2002)
albomarginata taken against peptic ulcers;
(C.B. Clarke) Ching, powdered leaves mixed
Pteridaceae with Zingiber officinale
Roscoe. rhizomes used
against infertility in women
and infusion taken against
dysentery
Ampelopteris prolifera Tanzania Leaf sap mixed with leaves Nwosu (2002)
(Retz.) Copel., of Hypoestes aristata
Thelypteridaceae (Vahl) Sol. ex Roem. &
Schult. and drunk to treat
meningitis and encephalitis
Anemia vestita (Baker) Swaziland Used to rid children of Long (2005)
Christenh., Anemiaceae worms and mixed with fat
for burns
Arthromeris wallichiana Nigeria Leaf paste applied Nwosu (2002)
(Spr.) Ching, externally for sprains, paste
Polypodiaceae mixed with egg albumen
applied to fractured bone
and used for calcinosis
Arthropteris palisotii Ivory Coast Leaf juice used for Bouquet (1974)
(Desv.) Alston, treatment of faints
Tectariaceae
Asplenium achilleifolium Nigeria Decoction of leaves and Nwosu (2002)
(M. Martens & Galeotti) roots drunk as health tea
Liebm., Aspleniaceae and purge; root powder
mixed with Elaeis
guineense Jacq. oil taken
internally for stomachache
and intestinal worms
(continued)
388 A. Maroyi
Table 1 (continued)
Asplenium adiantum- Nigeria Leaf extract mixed with Nwosu (2002)
nigrum L., Aspleniaceae Ricinus communis L. oil
and used as purge to
remove intestinal worms;
leaf decoction taken against
urinary disorders; tonic
used as antiseptic after
ear-piercing
South Africa Anthelmintic and emetic Watt and
Brandwijk
(1962)
Asplenium africanum DRC Leaf infusion used as Terashima et al.
Desv., Aspleniaceae enema for spleen disease (1991)
Gabon Whole plant used as herbal Sassen and Wan
medicine (2006)
Asplenium blastophorum Tanzania Pounded leaves mixed with Matthews
Hieron., Aspleniaceae cold water applied to stiffy (1993)
neck
Asplenium bulbiferum Nigeria Leaf paste applied Nwosu (2002)
G. Forst., Aspleniaceae externally for hemorrhoids;
leaf decoction taken for
liver problems
Asplenium cuneatum South Africa Emetic Watt and
Lam., Aspleniaceae Brandwijk
(1962)
Asplenium monanthes L., Lesotho Leaves smoked for head Moteetee and
Aspleniaceae and chest colds van Wyk (2011)
South Africa Leaves smoked for head Hutchings
and chest colds and also as (1992)
diaphoretic
Asplenium trichomanes L., Lesotho Leaves smoked for head Moteetee and
Aspleniaceae and chest colds van Wyk (2011)
South Africa Leaves smoked for head Hutchings
and chest colds (1992)
Blotiella glabra (Bory) DRC Leaves used for Termote (2012)
R.M. Tryon, menstruation pain
Dennsteadtiaceae
Botrychium lanuginosum Nigeria Boiled rhizomes used for Nwosu (2002)
Wall. ex Hook et Grev., pneumonia, catarrh and
Ophioglossaceae expectorant
Cheilanthes albomarginata Nigeria Leaf extract used externally Nwosu (2002)
C.B. Clarke, Pteridaceae for abscess and decoction
of rhizomes administered
internally against diarrhoea
(continued)
Table 1 (continued)
Cheilanthes eckloniana Lesotho Leaves smoked for head Moteetee and
Mett., Pteridaceae and chest colds van Wyk (2011)
South Africa Leaves smoked for head van Wyk et al.
and chest colds and ashes (2013)
from burnt leaves used on
sores
Swaziland Leaves smoked for head Long (2005)
and chest colds and ashes
from burnt leaves used for
sores
Cheilanthes farinosa Ethiopia Leaf powder crushed with Yineger et al.
(Forssk.) Kaulf., leaves of Rhamnus (2007)
Pteridaceae prinoides L’Hér. and the
whole plant of Crepis
rueppellii Sch. Bip. and
brushed over the body as
remedy for hepatititis
Uganda Decoction of leaves, roots Tabuti et al.
and stems used for (2012)
headache, paludism and
gastritis
Cheilanthes hirta Sw., Lesotho Decoction drunk for colds Moteetee and
Pteridaceae and sore throat van Wyk (2011)
South Africa Used as anthelmintic, sore Roux (2003) and
throats, head and chest van Wyk et al.
colds. Burned with Mohria (2013)
caffrorum (L.) Desv. as an
inhalation for a restless
child and rhizomes used as
cancer cure
Swaziland Decoction used for sore Roux (2003) and
throat, colds and asthma Long (2005)
Cheilanthes involuta (Sw.) Lesotho Leaves smoked for head Moteetee and
Schelpe & N.C.Anthony, and chest colds van Wyk (2011)
Pteridaceae South Africa Leaves smoked for head van Wyk et al.
and chest cold (2013)
(continued)
390 A. Maroyi
Table 1 (continued)
Cheilanthes quadripinnata Lesotho Rhizome mixed with milk Moteetee and
(Forssk.) Kuhn, drunk for diarrhoea and van Wyk (2011)
Pteridaceae spider bites
South Africa Rhizome decoction injected Watt and
into the vagina of a cow if Brandwijk
it does not get rid of the (1962) and Roux
placenta after calving. (2003)
Crushed leaves mixed with
milk and used for diarrhoea
and spider bites
Swaziland Rhizome mixed with milk Long (2005)
used for diarrhoea
Cheilanthes spp., Lesotho Whole plant slightly Shale et al.
Pteridaceae roasted and powdered (1999)
material applied to wounds
Cheilanthes viridis Madagascar Aerial parts used as Jenkins (1987)
(Forssk.) Swart, diuretics
Pteridaceae South Africa Paste made from dried Hutchings et al.
ground leaves applied to (1996)
burns, sores and other skin
complaints
Swaziland Paste made from dried Roux (2003) and
ground leaves applied to Long (2005)
burns, sores and other skin
complaints
Coniogramme caudata Nigeria Leaf extract taken as Nwosu (2002)
Ching, Adiantaceae remedy for urinary
disorders, backache and
gall stones
Cyathea dregei Kunze, South Africa Infusions taken regularly Hutchings et al.
Cyatheaceae during pregnancy to ensure (1996)
easy childbirth and dried
roots used as anthelmintics
Swaziland Tonic taken in pregnancy to Long (2005)
ensure easy birth; roots
taken for toothache and
anthelmintic
Cyathea manniana Hook., DRC Leaves used for Termote (2012)
Cyatheaceae menstruation pain
Ethiopia Leaf decoction used for Tesfaye and
sexually transmitted Sebsebe (2009)
diseases
South Africa Leaf decoction used as Watt and
worm remedy Brandwijk
(1962)
(continued)
Table 1 (continued)
Cystopteris fragilis Bernh., Lesotho Rhizome decoction used as Moteetee and
Woodsieaceae enema for deworming and van Wyk (2011)
constipation
Dicranopteris linearis Nigeria Extract of whole plant Nwosu (2002)
(Burm. f.) Underw., administered as remedy for
Gleichenaceae convulsions in children
Diplazium esculentum Nigeria Leaf infusion used for Nwosu (2002)
(Retz.) Sw., Athyriaceae toothache and by pregnant
women as protection
against difficult childbirth
Diplazium sammatii DRC Roots used for Termote (2012)
(Kuhn) C.Chr., Athyraceae menstruation pain
Dryopteris athamantica South Africa Used as general Hutchings et al.
(Kunze) Kuntze, anthelmintic and rhizome (1996)
Dryopteridaceae decoction used for retained
placenta in cows
Swaziland Rhizome used as a general Long (2005)
anthelmintic
Dryopteris filix-mas (L.) Nigeria Leaves used as vermifuge Nwosu (2002)
Schott, Dryopteridaceae and leaf infusion used as
aphrodisiac
Dryopteris inaequalis Ethioipia Root decoction used as Yineger et al.
(Schlechtd.) Kuntze, ethnoveterinary medicine (2007)
Dryopteridaceae Kenya Herbal medicine, uses not Timberlake
specified (1994)
South Africa Rhizome taken as Hutchings et al.
anthelmintic (1996)
Dryopteris lewalleana Pic. South Africa Rhizome used as an Roux (2003)
Serm., Dryopteridaceae anthelmintic
Swaziland Rhizome used as a general Long (2005)
anthelmintic
Dryopteris pentheri Burundi Used against tenia and Baerts and
(Krasser) C. Chr., parasites Lehmann (1996)
Dryopteridaceae Swaziland Rhizome used as a general Long (2005)
anthelmintic
Dryopteris wallichiana Nigeria Tonic from roots drunk Nwosu (2002)
(Spreng) Hyl., against rheumatism
Dryopteridaceae
Elaphoglossum petiolatum South Africa Root decoction used for Watt and
Bonap., Lomariopsidaceae sore throat Brandwijk
(1962)
Equisetum diffusum Nigeria Root decoction used to treat Nwosu (2002)
D. Don, Equisetaceae psychosis and dried leaf
powder mixed with water
used as insect repellant
(continued)
392 A. Maroyi
Table 1 (continued)
Equisetum ramosissimum Lesotho Rhizome decoction drunk Moteetee and
Desf., Equisetaceae for infertility in women, van Wyk (2011)
colds and colic in infants
South Africa Plant sap used to relieve Hutchings et al.
toothache and applied to (1996) and Roux
wound after extraction. (2003)
Powdered stem and water
or milk infusions
administered as enemas to
children with abdominal
upsets. Rhizome decoctions
used for fertility, colds,
venereal diseases,
diarrhoea, septic
inflammations, glandular
swellings, earache and
diuretics. Roots crushed
and smoked as remedy for
asthma
Swaziland Cooked rhizome enhances Long (2005)
fertility in women
Glaphyropteridopsis Nigeria Leaf decoction used against Nwosu (2002)
erubescens (Wall ex indigestion; leaf paste
Hook.) Ching, applied externally for
Thelypteridaceae rheumatism; root powder
mixed with water and used
as antidote for scorpion bite
Lycopodiella cerma (L.) DRC Flea repellant Lemmens
Pic. Serm., Lycopodiaceae (2008)
Madagascar Decoction used as tonic and Lemmens
a mixture with Tristemma (2008)
mauritianum J.F. Gmel.
used to treat neuralgia and
hypertension; whole plant
used to prepare tea that is
drunk for stomach ulcers
Rwanda Whole plant crushed and Lemmens
applied as dressing to (2008)
wounds
Lycopodium cernuum L., DRC Whole plant used as insect Lemmens
Lycopodiaceae repellant (2008)
(continued)
Table 1 (continued)
Lycopodium clavatum L., DRC Whole plant placed on the Yamada (1999)
Lycopodiaceae bed like a mattress to keep
insects away
Lesotho Leaves dried and smoked Zimudzi and
for headache, colds in the Bosch (2008)
head and chest. Whole and Moteetee
plant dried and smoked and van Wyk
with Selaginella caffrorum (2011)
(Milde.) Hieron. to cure
head and chest colds
Madagascar Used as neuralgia; whole Jenkins (1987)
plant is grilled with and Zimudzi and
sugarcane and banana skins Bosch (2008)
and applied to cracked lips
to promote healing.
Nigeria Leaf decoction applied Nwosu (2002)
externally to wounds or
ulcer; pulverized leaves
mixed with unripe Musa
acuminata Colla mixed
with milk given in small
doses to infants suffering
from diarrhoea and
dysentery
Rwanda Extract of aerial parts drunk Zimudzi and
as cure for dysentery and Bosch (2008)
malaria
South Africa Whole plant smoked for Hutchings et al.
headaches and used as (1996)
decoction diuretic and
anti-spasmatic
Swaziland Used to increase urine flow Long (2005)
and relieve spasms
Lycopodium selago L., Nigeria Spores and root powder Nwosu (2002)
Lycopodiaceae mixed with Elaeis
guineense Jacq. oil and
applied externally to treat
eczema, cuts; leaf extract
drunk against snake bite
and as antidote
Lygodium flexuosum (L.) Nigeria Leaf infusion used for Nwosu (2002)
Sw., Lygodiaceae treating female infertility;
paste applied to fix
fractured bones and against
hemorrhoids
Lygodium microphyllum Ivory Coast Crushed leaves used to cure Bouquet (1974)
(Cav.) R. Br., Lygodiaceae hiccough
(continued)
394 A. Maroyi
Table 1 (continued)
Lygodium palmatum Nigeria Leaf extract applied Nwosu (2002)
(Bernh.) Sw., Lygodiaceae externally to boils, abscess,
swellings; used as
astringent and emollient
Marattia fraxinea Sm., DRC Root infusion used as Terashima et al.
Marattiaceae enema for pregnant women (1991)
near delivery and for those
who suffer from abdominal
troubles
Tanzania Remedy for Watt and
ankylostomiasis Brandwijk
(1962)
Marsilea minuta L., Nigeria Plant extract used as van der Burg
Marsileaceae aphrodisiac and for (2004a)
increased fertility
Marsilea quadrifolia L., Nigeria Whole plant extract used as Nwosu (2002)
Marsileaceae aphrodisiac and for
increased fertility by
married couple
Microgramma South Africa Whole plants used against Hutchings et al.
lycopodioides (L.) Copel., public lice (1996)
Polypodiaceae Tanzania Crushed leaves mixed with Matthews
porridge used as remedy for (1993)
anaemia and stomach pains
Microgramma mauritiana South Africa Whole plant used against Hutchings et al.
(Willd.) Tardieu, lice on humans (1996)
Polypodiaceae Swaziland Whole plant used against Roux (2003) and
lice on humans Long (2005)
Microgramma owariensis DRC Women put this fern on Yamada (1999)
(Desv.) Alston, their body for 3 days as
Polypodiaceae remedy for menorrhagia
and used for abdominal
disorder
Microsorum punctatum DRC Leaf decoction used as Yamada (1999)
(L.) Copel, Polypodiaceae enema
Ivory Coast Leaves and juice purgative, Bouquet (1974)
diuretic and wound healing
Mohria caffrorum (L.) South Africa Smoke of burnt leaf is Hutchings et al.
Desv., Anemiaceae inhaled by children for (1996) and van
nightmares, dried leaf Wyk et al.
ointment used as cooling (2013)
ointment for burns and
leaves smoked for head and
chest colds. Burned with
Cheilanthes hirtaSw. as an
inhalation for a restless
child
(continued)
Table 1 (continued)
Mohria vistata Baker, South Africa Plant said to rid children of Roux (2003)
Anemiaceae worms and the ground
leaves are mixed with fat
and used as an ointment
against burns
Nephrolepsis acutifolia Gabon Whole plant used as herbal Sassen and Wan
(Desv.) Christ, medicine (2006)
Davalliaceae
Nephrolepsis biserrata Cameroon Leaf decoction used for Jiofack et al.
(Sw.) Schott, Davalliaceae abdominal pains (2008)
DRC Leaves used to remove Termote (2012)
thorns in the body
Ivory Coast Leaves used as enema or Malan and
therapeutic meal during Neuba (2011)
pregnancy and wounds
Liberia Snake bites and urinary Harley (1941)
complaints
Nephrolepsis cordifolia Nigeria Leaf infusion administered Nwosu (2002)
(L.) Presl., Davalliaceae for amnesia
Nephrolepsis undulata Gabon Whole plant used as herbal Sassen and Wan
(Afzel. ex Sw.) J. Sm., medicine (2006)
Davalliaceae
Ophioglossum capense South Africa Rhizome decoction used as Watt and
Sw., Ophioglossaceae remedy for boils Brandwijk
(1962)
Ophioglossum costatum Madagascar Rhizome decoction taken van der Burg
R.Br., Ophioglossaceae internally to treat lung and (2004b)
heart diseases; dried and
pulverized rhizome applied
to sores, wounds and burns
Ophioglossum reticulatum Gabon Whole plant used as herbal Sassen and Wan
L., Ophioglossaceae medicine (2006)
Lesotho Rhizome decoction used van der Burg
topically on boils (2004b)
Nigeria Rhizome extract used as van der Burg
antidote for snake bite (2004b)
Tanzania Leaf juice drunk against van der Burg
spasms of the heart (2004b)
Ophioglossum vulgatum Lesotho Rhizome decoction used to Moteetee and
L., Ophioglossaceae bathe boils van Wyk (2011)
Nigeria Rhizome decoction taken Nwosu (2002)
internally to treat
pulmonary, bronchial and
heart diseases; dried
pulverized rhizome
externally applied to
ulcerated sores, burns and
wounds
(continued)
396 A. Maroyi
Table 1 (continued)
Osmunda regalis L., Nigeria Whole plant extract taken Nwosu (2002)
Osmundaceae internally for psychosis;
infusion of roots used
against malaria and
jaundice
Pellaea calomelanos (Sw.) Kenya Herbal medicine, uses not Timberlake
Link., Pteridaceae specified (1994)
Lesotho Cooked rhizome decoction Moteetee and
used to get rid of afterbirth van Wyk (2011)
in cows and colds in
humans
Malawi Whole plant burnt and Gelfand et al.
smoke directed into vagina (1985)
as remedy for painful
uterus
South Africa Smoke from burnt leaves Hutchings et al.
inhaled for headaches, (1996) and
asthma, head colds and leaf Semenya and
decoction taken for Maroyi (2013)
diarrhoea, coughs and
colds. Rhizome decoctions
taken or applied to boils
and mouth or nasal ulcers
while boiled roots and
rhizomes are used for
tuberculosis and as
poultices for abscesses.
Milk decoctions of
rhizomes used for internal
sores and parasites
Swaziland Rhizome decoction used for Roux (2003) and
sores and boils Long (2005)
Zimbabwe Powder of whole plant Gelfand et al.
taken in porridge to prevent (1985)
abortion or smoke directed
into vagina to prevent
abortion. Infusions of
whole plant taken by mouth
and plant also burnt and
smoke inhaled as remedy
for convulsions. Ointment
of whole plant applied to
depressed fontanelle.
Whole plant mixed with
roots of Peltophorum
africanum Sond. and the
powder taken in porridge as
remedy for abdominal pains
(continued)
Table 1 (continued)
Pellaea involuta (Sw.) South Africa Rhizome used for spider Watt and
Baker, Pteridaceae bite and diarrhoea Brandwijk
(1962)
Pellaea longipilosa Kenya Herbal medicine, uses not Timberlake
Bonap., Pteridaceae specified (1994)
Pellaea rufa A.F. Tryon, South Africa Roots used for sores and Hutchings et al.
Pteridaceae skin complaints (1996)
Pellaea spp., Pteridaceae Zimbabwe Fronds and roots burnt and Maroyi (2013)
smoke inhaled as remedy
for chest pains
Polypodiodes Nigeria Leaf and rhizome decoction Nwosu (2002)
microrhizoma (C. B. used for backache,
Clarke ex Baker) Ching, gastrointestinal disorders
Polypodiaceae and jaundice; leaf paste
applied externally to
fissures on hand and
wounds; paste mixed with
palm-kernel oil applied
externally to sheep or cattle
Polystichum pungens Lesotho Rhizome decoction used as Moteetee and
(Kaulf.) C.Presl, vermifuge van Wyk (2011)
Dryopteridaceae South Africa Rhizome decoctions Hutchings et al.
administered as enemas for (1996)
intestinal worms and
wounds
Pteridium aquilinum (L.) Angola Leaves and stems used for Hutchings et al.
Kuhn, Pteridaceae toothache and madness (1996)
DRC Leaves used for eye injuries Terashima et al.
and young buds to remove (1991) and
thorns in feet; leaf infusion Termote (2012)
given by enema as a
purgative for cleaning
bowels. Shoot pounded to a
paste and applied to
swellings.
Ivory Coast Pulp of cooked crosiers van der Burg
used as enema to overcome (2004c)
sterility in women.
Rhizome mixed with
rhizome of Zingiber
officinale Roscoe pounded
and juice drunk as
aphrodisiac
Nigeria Rhizome decoction drunk Nwosu (2002)
as herbal tea and purge
(continued)
398 A. Maroyi
Table 1 (continued)
South Africa Root decoction used as Hutchings et al.
vermifuge and mixed with (1996) and Roux
rootstock of (2003)
Gymnanthemum
corymbosum (Thunb.)
H. Rob. for menstrual
irregularities and as
abortifacient. Rhizome sap
used for chronic septic
sores. Rhizome used as
anthmelmintic for livestock
and for stomachache and
diarrhoea in humans. Stem
sap used for insect bites,
stings and rashes.
Decoction of roots taken
orally to facilitate
trouble-free childbirth and
during painful and extended
labour
Swaziland Roots make teas for rickets, Long (2005)
stomach cramps, diarrhoea,
worms and male sexual
impotence
Tanzania Leaf decoction used for Kisangau et al.
oral candidiasis and (2011)
tuberculosis
Pteris prolifera Hieron., DRC Whole plant used as an Yamada (1999)
Pteridaceae insect repellant
Pteris quadriaurita Retz., Nigeria Crushed rhizome used as Nwosu (2002)
Pteridaceae astringent and emollient
and extract of leaves mixed
with Elaeis guineense Jacq.
used as insect repellant
Selaginella caffrorum Lesotho Whole plant dried and Moteetee and
(Milde.) Hieron., smoked with Lycopodium van Wyk (2011)
Selaginellaceae clavatum L. to cure head
and chest colds
South Africa Whole plant mixed with Hutchings et al.
Lycopodium clavatum L. or (1996)
Selaginella sellowii Hieron.
smoked for headaches;
decoction diuretic and
anti-spasmatic
(continued)
Table 1 (continued)
Selaginella cinerascens Nigeria Dried rhizomes and spores Nwosu (2002)
A. A. Eaton, mixed with boiled water
Selaginellaceae used as douche and
emollient; leaf infusion
drunk to relieve
gastrointestinal disorders
such as heart burns and
atrophy
Selaginella dregei South Africa Whole plant burnt and van Wyk and
(C. Presl) Hieron., smoke directed into mouth Gericke (2007)
Selaginellaceae for toothache
Selaginella fissidentodes Madagascar Leaf decoction used for de Winter and
(Hook. & Grev.) Spring, cough Jansen (2003)
Selaginellaceae
Selaginella myosurus Cameroon Whole plant maceration Jiofack et al.
Alston, Selaginellaceae used for headache (2008)
Selaginella pallescens Nigeria Leaf paste applied Nwosu (2002)
(C. Presl) Spring, externally on joints to treat
Selaginellaceae rheumatism; rhizome
infusion drunk against
insomnia; young leaves
steamed mixed with
Vitellaria paradoxa C. F.
Gaertn. and used for
hemorrhoids as ointment. It
is applied on fistulas
Selaginella sellowii South Africa Whole plant smoked for Hutchings et al.
Hieron., Selaginellaceae headaches; decoction (1996)
diuretic and anti-spasmatic
Selaginella spp., DRC Dried leaves of Afromomum Terashima et al.
Selaginellaceae laurentii (De Wild. & (1991)
T. Durand) K. Schum. and
the resin of Canarium
schweinfurtii Engl. are used
in baths for measles. The
plant is used for snake bites
Selaginella tenuissima Nigeria Leaf decoction drunk Nwosu (2002)
Fée, Selaginellaceae against liver problems,
hepatoma; leaf paste mixed
with ashes of Elaeis
guineense Jacq. applied
externally to tattooed skin
as an antiseptic and
cosmetics
Selaginella vogelii Mett., Cameroon Whole plant maceration Jiofack et al.
Selaginellaceae used for kidney problems, (2008)
skin and cutaneous diseases
(continued)
400 A. Maroyi
Table 1 (continued)
Stenochlaena tenuifolia Congo Sap taken with ripe banana van der Burg
(Desv.) T.Moore, (Brazzaville) as aphrodisiac (2004d)
Blechnaceae
Tectaria coadunata (Wall. Nigeria Leaf powder mixed with Nwosu (2002)
ex Hook. & Grev.) C. Chr., Ricinus communis L. oil
Dryopteridaceae and given to goats and
sheep for running stomach;
young leaves chewed by
cow after delivery to
accelerate the expulsion of
the placenta; root paste
applied externally to stop
foot itching caused by
fungal growth and root
infusion drunk against
gonorrhea
Thelypteris dentata Tanzania Leaves crushed and applied Chhabra et al.
(Forssk.) E. P. St. John, over body parts against (1987)
Thelypteridaceae bacterial and fungal
infections
Vittaria elongata Sw., Tanzania Leaves cooked in water Matthews
Pteridaceae then rubbed over patient as (1993)
remedy for fever
Woodwardia unigemmata Nigeria Infusion of spores and Nwosu (2002)
(Makino) Nakai, rhizomes administered
Blechnaceae internally for ringworm;
leaf decoction used as tea
as well as bath for
infertility in women,
abdominal pain,
constipation and sore throat
animal ailments and diseases. Aspleniaceae, Polypodiaceae, Pteridaceae and

Selaginellaceae families have at least five species used for medicinal purposes
(Fig. 2a). Members of these pteridophyte families are also used in other regions of
the world as herbal medicines, for example, Aspleniaceae, Polypodiaceae and
Selaginellaceae are among taxa employed as herbal medicines in northeast Brazil
(Agra et al. 2008). Genera with the highest number of species used for medicinal
purposes are Asplenium and Selaginella, represented by at least six species each
(Fig. 2b). The medicinal value of the genus Asplenium was early recognized by
taxonomists, as the name “Asplenium” comes from the Greek word “splen”, for “spleen”,
denoting the ancient medicinal use of the genus in treating diseases of the spleen
(Yarborough and Powell 2002). Members of the genus Selaginella have high
pharmaceutical value due to the presence of a variety of secondary metabolites
such as alkaloids, phenol, terpenoids and some species demonstrated anti-inflam-
matory, antibacterial, antifungal, antiviral, antimicrobial and antioxidant activities
A B
Thelypteridaceae Pellaea
Ophioglossaceae Pteris
Davallaceae Microgramma
Lycopodiaceae Lycopodium
Polypodiaceae Nephrolepsis
Dryopteridaceae Dryopteris
Aspleniaceae Cheilanthes
Adiantaceae Adiantum
Selaginaceae Asplenium
Pteridaceae Selaginella
0 5 10 15 20 0 2 4 6 8 10 12
Number of species Number of species
Fig. 2 Pteridophyte families (a) and genera (b) with the largest number of species used for medic-
inal purposes in sub-Saharan Africa
(Setyawan 2009). Other important genera include Adiantum, Cheilanthes,

Dryopteris, Lycopodium, Microgamma, Nephrolepsis, Pallaea and Pteris (Fig. 2b).
Pteridophytes used for medicinal purposes in sub-Saharan Africa have been
recorded from 20 countries (Fig. 3). Most of the ethnobotanical data on medicinal
plants have been reported in DRC, Gabon, Ivory Coast, Lesotho, Madagascar,
Nigeria, South Africa, Swaziland and Tanzania (Table 1), all these countries have
miombo woodland or mountainous areas characterized by small pockets of afromon-
tane forests or tropical rain forests, the natural habitats of pteridophytes (Kornaś
1993). Low pteridophyte diversity of sub-Saharan Africa is mainly the result of the
paucity of the rain forest flora (Aldasoro et al. 2004). In Nigeria, 37 pteridophyte
species are used for medicinal purposes, followed by South Africa where 33 species
are used, Lesotho and Swaziland with 16 species each, DRC with 14 species and the
rest of the other countries haveless than 10 species each (Fig. 3). Popular pterido-
phyte species used for medicinal purposes in at least three countries include
Adiantum capillus-veneris (Lesotho, Nigeria, South Africa, Swaziland), Cheilanthes
eckloniana (Lesotho, South Africa, Swaziland), Cheilanthes hirta (Lesotho, South
Africa, Swaziland), Cheilanthes quadripinnata (Lesotho, South Africa, Swaziland),
Cheilanthes viridis (Forssk.) Swart (Madagascar, South Africa, Swaziland), Cyathea
manniana Hook. (DRC, Ethiopia, South Africa), Dryopteris inaequalis (Ethiopia,
Kenya, South Africa), Equisetum ramosissimum (Lesotho, South Africa, Swaziland),
Lycopodiella cerma (L.) Pic. Serm. (DRC, Madagascar, Rwanda), Lycopodium cla-
vatum L. (DRC, Lesotho, Madagascar, Nigeria, Rwanda, South Africa, Swaziland),
Nephrolepsis biserrata (Sw.) Schott (Cameroon, DRC, Ivory Coast, Liberia),
Ophioglossum reticulatum L. (Gabon, Lesotho, Nigeria, Tanzania), Pellaea calo-
melanos (Kenya, Lesotho, Malawi, South Africa, Swaziland, Zimbabwe) and
Pteridium aquilinum (L.) Kuhn (Angola, DRC, Ivory Coast, Nigeria, South Africa,
402 A. Maroyi
Fig. 3 Pteridophyte species used for medicinal purposes in sub-Saharan Africa with forests in
green
Swaziland, Tanzania) (Table 1). Only two pteridophyte species are used for cultural
and ritualistic purposes in at least three countries: Equisetum ramosissimum
(Lesotho, South Africa, Swaziland) and Pteridium aquilinum (DRC, Ivory Coast,
Uganda) (Table 1).
About 9.4% of the pteridophyte species documented in this study are sold by
traditional medicine sellers in sub-Saharan Africa (Table 2). The commercialization
of medicinal plants is a strategy that is employed by poor people in developing
countries as a means of generating income (Cunningham 1993). Research has
shown that millions of people from sub-Saharan Africa derive a significant part of
their income from gathered wild plants (Williams et al. 2001). Although, the contri-
bution of medicinal plants to household income is widely recognized (Cunningham
1993), there are few studies that have examined the significance of the trade in
pteridophytes to the livelihoods of poor and marginalized people as well as their
conservation status throughout their geographical range. Knowing what species are
traded commercially is the foundation for identifying taxa threatened by the trade
and comparisons with other regional and national medicinal plant markets (Williams
et al. 2001). Conservation assessments made in southern Africa (Bingham and
Smith 2002; Mapaura and Timberlake 2002; Talukdar 2002) revealed that over-
Table 2 Pteridophytes sold by traditional medicine sellers in sub-Saharan Africa

Species Plant part(s) Country Reference(s)
Asplenium rutifolium Whole plant South Africa Cunningham (1993)
Cheilanhes hirta Leaves, roots South Africa Williams et al. (2001)
Cyathea dregei Roots, stem South Africa Cunningham (1993)
Dryopteris athamantica Roots South Africa Cunningham (1993)
Dryopteris inaequalis Roots South Africa Williams et al. (2001)
Equisetum Roots South Africa Williams et al. (2001)
ramosissimum
Lycopodium clavatum Whole plant South Africa, Cunningham (1993) and
Swaziland Williams et al. (2001)
Microgramma Whole plant South Africa Cunningham (1993)
lycopodioides
Pellaea calomelanos Leaves, roots, Zimbabwe Cunningham (1993) and
whole plant Williams et al. (2001)
Pellaea rufa Roots South Africa Cunningham (1993) and
Williams et al. (2001)
Selaginella imbricata Whole plant Zambia, Cunningham (1993)
Zimbabwe
exploitation of some of the documented pteridophyte species as herbal medicines,

limited distribution, habitat loss and modification are major causes of threats.
Acrostichum aureum is critically endangered in Zimbabwe due to severely frag-
mented habitat and continuing population decline (Mapaura and Timberlake 2002).
Cyathea dregei is also critically endangered in Lesotho due mainly to habitat degra-
dation (Talukdar 2002). Selaginella imbricata is vulnerable in Zambia due to a very
restricted area of occupancy and therefore prone to anthropogenic disturbances and
stochastic events (Bingham and Smith 2002). Although Pellaea rufa is not listed in
the Southern African Plant Red Data List, it is endemic to the Western Cape
Province, South Africa (Burrows 1990). Baseline data on collection of pteridophyte
species for medicinal value play an important role in drafting conservation plans for
such species in the regions where they occur.
Ailments and Diseases Treated by Pteridophyte Species
A total of 42 medical conditions are treated using remedies made from pteridophyte
species (Table 1). Cold, cough and sore throat, injuries, parasitic worms, gastro-
intestinal system, gynaecological, general body pains, inflammations, urinary sys-
tem, respiratory system, ethno-veterinary medicine, insect repellent, pregnancy,
birth and puerperium, snake and spider antidote, headache, circulatory system, der-
matological, fainting and fits, fever, hair lice and dendrufs, odontological and sexual
dysfunctional are treated with the highest number of medicinal pteridophytes in
404 A. Maroyi
Table 3 Major ailments and disease categories and pteridophyte species reported
Ailment category Number of pteridophyte species
Cold, cough and sore throat 24
Injuries 23
Parasitic worms 19
Gastro-intestinal system 16
Gynaecological 13
General body pains 11
Inflammations 11
Urinary system 9
Respiratory system 8
Ethnoveterinary medicine 8
Insect repellent 7
Pregnancy, birth and puerperium 7
Snake and spider antidote 7
Headache 6
Circulatory system 5
Dermatological 5
Fainting and fits 5
Fever 5
Hair lice and dendrufs 5
Odontological 5
Sexual dysfunctional 7
Most species are reported in more than one ailment and disease category
sub-Saharan Africa (Table 3). For hundreds of years, indigenous people in southern

Africa, particularly South Africa have used a variety of pteridophytes species as
herbal medicines for coughs, colds and respiratory infections and gastro-intestinal
problems (Watt and Brandwijk 1962; Hutchings et al. 1996; van Wyk et al. 2013).
Injuries and wound infections are one of the most common diseases in developing
countries mainly because of poor hygienic conditions (Ayyanar and Ignacimuthu
2009). Leaf decoction of Pteridium aquilinumis used to treat and manage oral can-
didiasis in Tanzania (Kisangau et al. 2011), which is one of the prevalent HIV/AIDS
opportunistic infections in sub-Saharan Africa (Maroyi 2014b). Almost all plant
parts are used to prepare different remedies: leaves, rhizomes, roots, sap and spores.
Plant parts most frequently used in preparing herbal medicines are leaves (44.4%),
whole plant (39.3%) and rhizome and/or roots (36.8%) (Table 1). Monotherapy
preparations made from a single pteridophyte species are the most dominant
(84.6%), while 12.8% and 2.6% of the herbal concortions are prepared from a com-
bination of two and three species respectively (Table 1). Plant remedies are often
utilized in the form of decoction, infusion or maceration (47.9%), sap or paste
(33.3%) and smoked or smoke inhaled (19.7%) (Table 1). Combination of at least
two herbal medicines (Table 1) is believed to increase efficacy of the herbal concor-
tions due to the additive or synergistic effects that herbal medicines have during
disease or ailment treatment (Bussmann and Sharon 2006).
4 Conclusion
The annotated checklist in Table 1 provides baseline data on pteridophytespecies

used for medicinal purposes in sub-Saharan Africa. Published accounts of this cat-
egory of pteridophytes are available from Angola, Burundi, Cameroon, Congo,
DRC, Ethiopia, Gabon, Ivory Coast, Kenya, Lesotho, Liberia, Madagascar, Malawi,
Nigeria, Rwanda, South Africa, Swaziland, Tanzania, Uganda, Zambia and
Zimbabwe only. Although the results from these 20 countries are diverse in nature,
they may not apply directly to 48 countries in the sub-Saharan African region. Based
on the results of this study, it appears that the use of pteridophytes for medicinal
purposes is entrenched in cultural practices throughout the sub-Saharan African
region. Documentation of how local communities use pteridophytes and other plant
resources as herbal medicines is critical to safeguard indigenous knowledge and
conserve plant species which are rapidly being lost (Bingham and Smith 2002;
Mapaura and Timberlake 2002; Talukdar 2002). More importantly, documentation
of pteridophytes used for medicinal, cultural and ritualistic purposes can facilitate
future research on the safety and efficacy of these plant resources. Poor health facili-
ties and limited access to drugs have perpetuated and increased the use of traditional
medicines particularly in rural and marginalized areas (Kisangau et al. 2011; Maroyi
2013). Studies like the current investigation can help stimulate confidence in the use
of traditional medicines among local communities and appreciate the value of plant
resources. Sharing of traditional knowledge on medicinal plants is important for
maintaining options for the use of traditional medicines, particularly as use of alter-
native medicine is growing because of its moderate costs and increasing faith in
herbal medicines. As demand for medicinal plants continue to accelerate, awareness
creation should be made among local communities to ensure sustainable use and
conservation of medicinal plants.
This inventory is a crucial starting point in trying to demonstrate that traditional
knowledge on pteridophytes used for medicinal purposes represent not only an
important heritage developed over centuries but it is also an important mass of data
which should be exploited in order to assist future workers with a repository of
herbal plants to evaluate for phytochemical safety and pharmaceutical efficacy.
Further ethnopharmacological studies are recommended for the documented pteri-
dophytes species as some of these species have already shown interesting applica-
tions in primary healthcare in several sub-Saharan African countries (Table 1).
There is need therefore, to assess phytochemical and pharmacological properties of
popular pteridophyte species used for medicinal purposes such as Adiantum
capillus-veneris, Cheilanthes eckloniana, Cheilanthes hirta, Cheilanthes quadri-
pinnata, Cheilanthes viridis, Cyathea manniana, Dryopteris inaequalis, Equisetum
ramosissimum, Lycopodiella cerma, Lycopodium clavatum, Nephrolepsis biserrata,
Ophioglossum reticulatum, Pellaea calomelanos and Pteridium aquilinum with the
aim of identifying active ingredients contained by such plants. Historically, active
compounds have been isolated from medicinal plants, and a landmark discovery
includes the discovery of quinine from the bark of the cinchona (quina-quina) tree
406 A. Maroyi
(Achan et al. 2011). Further investigations on phytochemical constituents and sub-

sequent screening are needed for opening new opportunities to develop pharmaceu-
tical drugs based on medicinal applications of pteridophytes in sub-Saharan African.
Acknowledgments The author is thankful to the Govan Mbeki Research and Development
Centre (GMRDC), University of Fort Hare, South Africa for funding the research project.
References
Achan J, Talisuna AO, Erhart A, Yeka A, Tibenderana JK, Baliraine FN, Rosenthal PJ, D’Alessandro
U (2011) Quinine, an old anti-malarial drug in a modern world: role in the treatment of malaria.
Malar J 10:144
Agra MF, Silva KN, Basílio IJLD, de Freitas PF, Barbosa-Filho JM (2008) Survey of medicinal
plants used in the region Northeast of Brazil. Braz J Pharmacog 18(3):472–508
Aldasoro JJ, Cabezas F, Aedo C (2004) Diversity and distribution of ferns in sub-Saharan Africa,
Madagascar and some islands of the South Atlantic. J Biogeogr 31(10):1579–1604
Ayyanar M, Ignacimuthu S (2009) Herbal medicines for wound healing among tribal people in
southern India: ethnobotanical and scientific evidences. Int J Appl Res Nat Prod 2(3):29–42
Baerts M, Lehmann J (1996) Plantes médicinales à effet placebo au Burundi. In: Schröder E,
Balansard G, Cabalion P, Fleurentin J, Mazars G (eds) Médicaments et aliments: Approche
ethnopharmacologique. Colloque Européen d’Ethnopharmacologie; Conférence Internationale
d’Ethnomédecine, Colloques et Séminaires, 24–27 March 1993, Heidelberg, pp 343–352
Bingham MG, Smith PP (2002) Zambia. In: Golding JS (ed) Southern African plant red data lists.
Southern African Botanical Diversity Network (SABONET) report no. 14, Pretoria, pp 135–156
Bouquet A (1974) Plantes medicinales de la Côte d’Ivoire. Oratom, Paris
Burrows JE (1990) Southern African ferns and fern allies. Frandsen Publishers, Johannesburg
Bussmann RW, Sharon D (2006) Traditional medicinal plant use in northern Peru: tracking two
thousand years of healing culture. J Ethnobiol Ethnomed 2:47
Chhabra SC, Mahunnah RLA, Mshiu EN (1987) Plants used in traditional medicine in eastern
Tanzania 1. Pteridophytes and angiosperms (Acanthaceae to Canellaceae). J Ethnopharmacol
21(3):253–277
Cook F (1995) Economic botany data collection standard. Royal Botanic Gardens, Kew
Cunningham AB (1993) African medicinal plants: setting priorities at the interface between con-
servation and primary healthcare. People and Plants Working Paper no. 1, UNESCO, Paris
de Winter WP, Jnasen PCM (2003) Selaginella Pal. Beauv. In: de Winter WP, Amoroso VB
(eds) Plant resources of south-east Asia 15(2) cryptogams: ferns and fern allies. Backhuys
Publishers, Leiden, pp 261–297
Fernández H, Kumar A, Revilla MA (2011) Working with ferns: issues and applications. Springer,
New York
Gelfand M, Drummnd RB, Mavi S, Ndemera B (1985) The traditional medical practitioner in
Zimbabwe: his principles of practice and pharmacopoeia. Mambo Press, Gweru
Harley GW (1941) Native African medicine: with special reference to its practice in the Mano tribe
of Liberia. Cambridge, MA
Harnett SM, Oosthuizen V, van de Venter M (2005) Anti-HIV activities of organic and aque-
ous extracts of Sutherlandia frutescens and Lobostemon trigonus. J Ethnopharmacol
96(1–2):113–119
Ho R, Teai T, Bianchini J-P, Lafont R, Raharivelomanana P (2010) Ferns: from traditional uses
to pharmaceutical development, chemical identification of active principles. In: Fernández
H, Kumar A, Revilla MA (eds) Working with ferns: issues and applications. Springer-Verlag,
Berlin, pp 321–346
Hutchings A (1992) Plants used for some stress-related ailments in traditional Zulu, Zhosa and
Sotho medicine. MSc dissertation, University of Natal, Pietermaritzburg
Hutchings A, Scott AH, Lewis G, Cunningham AB (1996) Zulu medicinal plants: an inventory.
University of Natal Press, Scottsville
Jenkins MD (1987) Madagascar: an environmental profile. IUCN, Gland
Jiofack T, Fokunang C, Kemeuze V, Fongnzossie E, Tsabang N, Nkuinkeu R, Mapongmetsem PM,
Nkongmeneck BA (2008) Ethnobotany and phytopharmacopoea of the south-west ethnoeco-
logical region of Cameroon. J Med Pl Res 2(8):197–206
Kisangau DP, Herrmann TM, Lyaruu HVM, Hosea KM, Joseph CC, Mbwambo ZH, Masimba
PJ (2011) Traditional knowledge, use practices and conservation of medicinal plants for HIV/
AIDS care in rural Tanzania. Ethnobot Res Appl 9:43–57
Kornaś J (1993) The significance of historical factors and ecological preference in the distribution
of African pteridophytes. J Biogeogr 20(3):281–286
Lemmens RMHJ (2008) Lycopodiella cerma (L.) Pic. Serm. In: Schmelzer GH, Gurib-Fakim A
(eds) Plant resources of tropical Africa 11: medicinal plants 1. PROTA Foundation, Leiden,
pp 365–366
Long C (2005) Swaziland’s flora: siSwati names and uses. http://www.sntc.org.sz/flora/clbotalpha.
asp?l=C&pg=10. Downloaded on 2 August 2014
Malan DF, Neuba DFR (2011) Traditional practices and medicinal plant use during pregnancy by
Anyi-Ndenye women (Eastern Côte d’Ivoire). Afr J Reprod Health 15(1):85–93
Mapaura A, Timberlake JR (2002) Zimbabwe. In: Golding JS (ed) Southern African plant red
data lists. Southern African Botanical Diversity Network (SABONET) report no. 14, Pretoria,
pp 157–186
Maroyi A (2013) Traditional use of medicinal plants in south-central Zimbabwe: review and per-
spectives. J Ethnobiol Ethnomed 9:31
Maroyi A (2014a) Not just minor wild edible forest products: consumption of pteridophytes in
sub-Saharan Africa. J Ethnobiol Ethnomed 10:78
Maroyi A (2014b) Alternative medicines for HIV/AIDS in resource-poor settings: insight from
traditional medicines use in sub-Saharan Africa. Trop J Pharm Res 13(9):1527–1536
Matthews P (1993) Medicinal plants of the Tanzanian coastal forest: a list of species with local
names and applications. The Society for Environmental Exploration, London
Moteetee A, van Wyk BE (2011) The medical ethnobotany of Lesotho: a review. Bothalia 41(1):209–228
Nwosu MO (2002) Ethnobotanical studies on some pteridophytes of southern Nigeria. Econ Bot
56(3):255–259
Puri HS (1970) Indian pteridophytes used in folk remedies. Amer Fern J 60(4):137–143
Rates SMK (2001) Plants as sources of drugs. Toxicon 39(5):603–613
Roux JP (2003) Swaziland ferns and fern allies. SABONET report 19, National Botanical Institute,
Pretoria
Sassen M, Wan M (2006) Biodiversity and local priorities in a community near the Ivindo national
park, Makokou, Gabon. Project IRET/CENAREST and CIFOR, Makokou.
Semenya SS, Maroyi A (2013) Medicinal plants used for the treatment of tuberculosis by Bapedi
traditional healers in the Limpopo Province, South Africa. Afr J Tradit Compl Altern Med
10(2):316–323
Setyawan AD (2009) Traditionally utilization of Selaginella: field research and literature review.
Nusantara Biosci 1(3):146–158
Shale TL, Stirk WA, van Staden J (1999) Screening of medicinal plants used in Lesotho for anti-
bacterial and anti-inflammatory activity. J Ethnopharmacol 67(3):347–354
Tabuti JRS, Kukunda CB, Kaweesi D, Kasib OMJ (2012) Herbal medicine use in the districts of
Nakapiripirit, Pallisa, Kanungu and Mukono in Uganda. J Ethnobiol Ethnomed 8:35
Talukdar S (2002) Lesotho. In: Golding JS (ed) Southern African plant red data lists. Southern
African Botanical Diversity Network (SABONET) report no. 14, Pretoria, pp 21–30
Terashima H, Kalaya S, Malasi N (1991) Ethnobotany of the Lega in the tropical rainforest of
eastern Zaire: part one, zone de Mwenga. Afr Stud Monogr 15(suppl.):1–61
408 A. Maroyi
Termote C (2012) Wild edible plant us in Tshopo District, Democratic Republic of Congo.
Ph.D.Thesis. Faculty of Bioscience Engineering, University of Ghent, Ghent
Tesfaye A, Sebsebe D (2009) Ethnobotanical study of medicinal plants in Kafficho people, south-
western Ethiopia. In: Svein E, Harald A, Birhanu T, Shiferaw B (eds) Proceedings of the 16th
international conference of Ethiopian studies. NTNU-Trykk Press, Trondheim, pp 711–726
Timberlake J (1994) Vernacular names and uses of plants in northern Kenya. J East Afr Nat Hist
83(1):31–69
van der Burg WJ (2004a) Marsilea minuta L. In: Grubben GJH, Denton OA (eds) Plant resources
of tropical Africa 2: vegetables. PROTA Foundation, Leiden, pp 379–380
van der Burg WJ (2004b) Ophioglossum reticulatum L. In: Grubben GJH, Denton OA (eds) Plant
resources of tropical Africa 2: vegetables. PROTA Foundation, Leiden, pp 404–405
van der Burg WJ (2004c) Pteridium aquilinum (L.) Kuhn. In: Grubben GJH, Denton OA (eds)
Plant resources of tropical Africa 2: vegetables. PROTA Foundation, Leiden, pp 439–441
van der Burg WJ (2004d) Stenochlaena tenuifolia (Desv.) T. Moore. In: Grubben GJH, Denton OA
(eds) Plant resources of tropical Africa 2: vegetables. PROTA Foundation, Leiden, pp 515–516
van Wyk B-E, Gericke N (2007) Peoples plants: a guide to useful plants of southern Africa. Briza
Publications, Cape Town
van Wyk B-E, van Oudtshoorn B, Gericke N (2013) Medicinal plants of South Africa. Briza
Publications, Cape Town
Watt JM, Brandwijk MG (1962) Themedicinal and poisonous plants of southern and eastern
Africa. E and S Livingstone, London
Williams VL, Balkwill K, Witkowski ETF (2001) A lexicon of plants traded in the Witwatersrand
umuthi shops, South Africa. Bothalia 31(1):71–98
World Health Organization (WHO) (2002) Traditional medicine strategy 2002–2005. WHO, Geneva
Yamada T (1999) A report on the ethnobotany of the Nyindu in the eastern part of the former Zaire.
Afr Stud Monogr 20(1):1–72
Yarborough SC, Powell AM (2002) Ferns and fern allies of the Trans-Pecos and adjacent areas.
Texas Tech University Press, Texas
Yineger H, Kelbessa E, Bekele T, Lulekal E (2007) Ethnoveterinary medicinal plants at Bale
Mountains National Park, Ethiopia. J Ethnopharmacol 112(1):55–70
Zimudzi C, Bosch CH (2008) Lycopodium clavatum L. In: Schmelzer GH, Gurib-Fakim A
(eds) Plant resources of tropical Africa 11: medicinal plants 1. PROTA Foundation, Leiden,
pp 366–369
Index
A 258, 260–262, 272, 273, 277, 278,

Africa, 1–15, 19, 61–71, 77–87, 98, 128, 144, 288–296, 299–301, 304, 305, 313, 317,
157, 232, 250, 283, 314, 345–354, 329, 331–334, 339, 405
357–371, 383–406 Benign prostatic hyperplasia, 11, 128, 131, 132
African cherry, 11, 127–139 Berchemia discolor, 143–153
African culture, 62–64, 79, 383 Bioactive compounds, 62, 203, 252, 254, 257,
African flora, 3, 20, 71, 79 260, 262, 264, 266, 272, 275, 285, 293,
Allium spp. 295, 297, 300, 302, 304, 346, 349,
A. ampeloprasum, 160, 163, 377–380 378, 380
A. roseum, 159, 160, 163, 377–380 Biodiversity, 7, 10, 232, 233, 244, 250, 279
Aloe species, 62, 63 Biodiversity hotspots, 4, 244
Antibacterial activity, 172, 177, 182, 186–188, Biological activities, 52, 80–87, 159, 165, 169,
191, 192, 241, 260, 269, 273, 275, 278, 185, 188, 250, 252, 253, 255, 258, 260,
291, 298, 305, 333 262, 264, 267, 271, 273, 275, 278, 286,
Antifungal, 45, 71, 103, 159, 169, 183, 186, 288, 291–293, 295, 298, 301, 305, 333,
188, 192, 195, 197, 199, 204, 234, 235, 346, 349–350, 352–353
253, 265, 273, 291, 298, 305, 358, Bird plum, 143–153
371, 400 Buchu, 23, 71
Antioxidant activity, 9, 14, 169, 174, 179, 180,
182, 189, 195, 198, 203, 260, 278, 293,
349, 352, 379 C
Argania spinosa, 25, 92–97 Candida albicans, 234, 236, 240, 242, 265,
Arid land, 173, 377, 379 269, 273, 275, 276, 292, 333, 368, 370
Aromatic and Medicinal Plants, 53–57 Capparis erythrocarpos, 359–371
Aromatic plants, 1–15, 19–58, 61–72, 77–87, Cedrus atlantica, 29, 92, 100–103, 171
98, 108, 158, 204 Central Africa, 57, 261, 278
Artemisia herba-alba, 25, 62, 70, 92, 97–100, Chemical entities, 9, 20, 52
160, 173, 186 Cultivated, 11, 20, 29, 33, 37, 43, 47, 48, 51,
64, 65, 80, 81, 91, 92, 110, 111, 114,
163, 166, 181, 182, 190, 192, 266,
B 311–341
Bark, 11, 21, 23, 25, 29, 31–33, 35–53, 55, 67, Cultivation, 9–11, 71, 111, 114, 139, 166, 181,
68, 81, 82, 85–87, 102, 104, 128, 278, 305, 314–316, 320–321, 325–327,
135–139, 144, 147–151, 164, 165, 167, 330–331, 335–337, 340, 341
235, 237, 239, 240, 243, 244, 254–256, Cultural and ritualistic uses, 402, 405

DOI 10.1007/978-94-024-1120-1
410 Index
D M
Dietary supplements, 2, 3, 12, 14, 29, 51, 52, MAPs. See Medicinal and aromatic
54, 178 plants (MAPs)
Dracaena steudneri, 360–371 Marula, 44, 67–68, 85
Mascarene Islands, 234, 235, 239
Mauritius, 8, 33, 231–244,
E 264, 304
East Africa, 11, 53, 57, 62, 68, 186, 264, 273, Medicinal, 1–15, 19–58, 61–72, 77–87,
277, 278, 287, 295, 357–371 91–121, 131–132, 147, 157–207,
Essential oils, 38, 42, 51, 52, 69–71, 91, 231–244, 250, 283–305, 311–341,
98–100, 102, 103, 106–111, 114–117, 346, 357–371, 377, 384
121, 158, 165, 173, 174, 176, 177, 181, Medicinal and aromatic plants (MAPs),
182, 184–187, 190, 191, 193–195, 200, 2, 7–14, 53–57, 157
202, 204, 207, 244, 252, 253, 272, 275, Medicinal plants (MP), 2, 3, 5–9, 12–14, 20,
285, 304, 305, 317, 325, 327, 328, 347, 53–57, 62–64, 91–121, 157–207,
348, 378 231–244, 262, 284, 311–341,
Ethnopharmacology, 79, 201, 233, 241, 346, 357–371, 401, 402, 405
371, 405 Monographs, 13, 26, 55, 79–87, 183
Morocco, 8, 24, 25, 29, 30, 33, 36, 37, 39–43,
47–49, 66, 67, 70, 91–121, 166, 167,
F 171, 173, 174, 179, 180, 182, 186, 196,
Food, 2, 12, 23, 26, 39, 63, 69, 91, 99, 107, 346, 349, 351
108, 120, 121, 144, 148, 149, 153, Myrtus communis, 39, 108–110
157–159, 166, 170, 175, 178, 180, 181,
183, 185, 186, 191, 192, 250, 253, 260,
273, 277, 284, 304, 311, 317, 318, 323, N
328, 332, 338–340, 349, 351, 354, 358, Nigeria, 7, 8, 21–24, 27, 30, 31, 33, 34,
376, 377, 380 38–40, 42–46, 48, 50, 51, 64, 66,
Functional food, 9, 20, 35, 45, 51, 54, 57, 163, 68, 72, 81, 128, 249–279,
193, 377, 379, 380 283–305, 311–341, 384,
386–401, 405
Nitraria, 345–354
H North Africa, 20, 28, 29, 32, 33, 49, 51,
Halophytes, 346 53, 56, 57, 62, 65–68, 98, 100, 109,
Henna, 65–66, 114 111, 116, 163, 164, 171, 173, 177,
Herbaceous plants, 97, 186, 249–279 182, 188, 190, 193–196, 201, 203–206,
Herbal medicine, 10, 13, 81, 87, 114, 128, 345–354
148, 163, 177, 233, 250, 284, 327, 328, Nutritional value, 153, 338, 377–378, 380
346, 378, 383–406
P
I Pharmacognostic effects, 250
Indian Ocean, 3, 51, 57, 232, 244, 264, Pharmacology, 7, 13, 143–153, 179
272, 289 Pharmacopoeia, 13–15, 51, 53, 55, 79, 109,
159, 171, 183, 186, 233, 257, 265,
286, 296
L Phytochemicals, 131, 132, 147, 163, 179,
Laurus azorica, 15, 103–108 187, 233, 243, 250, 252, 260, 262, 284,
Laurus nobilis, 37, 92, 103–108 305, 354
Livelihood needs, 153 Phytomedicines, 9, 14, 52, 57, 163
Index 411
Potential, 3, 5, 8–10, 13, 14, 53, 57, 135, 136, Therapeutics, 81, 203, 332, 339
139, 150, 158, 166, 167, 169, 170, 172, Traditional medicine (TM), 9, 12–14, 20,
177, 180, 185, 203, 234, 236, 238, 239, 51, 61–62, 68, 71–72, 78–87, 97,
242, 250, 268, 269, 278, 283–305, 312, 99, 107, 109, 157–207, 233, 239,
338, 346, 349, 352–354, 370, 371, 376, 241, 250, 252, 254, 258, 260,
378–380 262–263, 267, 272–273, 276,
Prunus africana, 11, 13, 40, 42, 52, 56, 57, 85, 278, 286, 291, 293, 296, 298,
127–139 300, 303, 305, 315, 331, 339,
Pteridophytes, 383–406 346, 358, 380, 384, 402, 403
Trees, 23, 36, 50, 67, 92–94, 103, 134–137,
148, 164, 196, 283–305, 331, 336,
R 337, 339
Rosa damascena, 92, 110–115 Tunisian, 157–207, 252, 348, 349, 352, 353,
Rosmarinus officinalis, 43, 70, 92, 108, 376, 378, 380
115–121, 160, 181, 182, 185
W
S West Africa, 4, 42, 51, 53, 57, 62–65, 68,
Secondary metabolites, 171, 176, 195, 200, 255, 257, 261, 262, 267, 271–274,
250, 346, 348, 400 277, 294, 295, 300, 314, 317,
Southern Africa, 2, 11, 20, 51, 53, 57, 62, 63, 331, 335, 338
67, 83–85, 325, 335, 402–404 Wild plants, 10, 11, 158, 166, 190, 191,
Sub-Saharan Africa, 20, 52, 53, 293, 299, 250, 402
337, 383–406 Wonder nut (Cola nitida), 30, 63–65
Sustainable management, 5–7
Z
T Zygophyllum, 161, 206–207, 345–354
Therapeutic potential, 283–305

Mohamed Neffati, Hanen Najja, Ákos Máthé (Ed.) - Medicinal and Aromatic Plants of The World - Africa Volume 3 (2017) PDF

Uploaded by

Copyright:

Available Formats

Mohamed Neffati, Hanen Najja, Ákos Máthé (Ed.) - Medicinal and Aromatic Plants of The World - Africa Volume 3 (2017) PDF

Uploaded by

Document Information

Original Title

Copyright

Available Formats

Share this document

Share or Embed Document

Sharing Options

Did you find this document useful?

Is this content inappropriate?

Copyright:

Available Formats

Mohamed Neffati, Hanen Najja, Ákos Máthé (Ed.) - Medicinal and Aromatic Plants of The World - Africa Volume 3 (2017) PDF

Uploaded by

Copyright:

Available Formats

Medicinal and Aromatic Plants of the World

Medicinal and Aromatic

ISSN 2352-6831 ISSN 2352-684X (electronic)

Library of Congress Control Number: 2017944445

© Springer Science+Business Media B.V. 2017

Printed on acid-free paper

This Springer imprint is published by Springer Nature

Médenine, Tunisia Mohamed Neffati

I ntroduction to Medicinal and Aromatic Plants in Africa........................... 1

 ome Wild Herbaceous Plants of Nigeria: A Biological,

Ákos Máthé, Mohamed Neffati, and Hanen Najjaa

© Springer Science+Business Media B.V. 2017 1

Pharmacopoeia is likely to mark an important milestone in meeting quality, safety

Keywords Medicinal and aromatic plants • Traditional medicine • African flora •

Remarkably, despite of the relevant dynamic increase in the number of publica-

Africa as a Botanical Continent

According to the World Geographical Scheme for Recording Plant Distributions

Table 1 Biodiversity hotspots in Africa

Biodiversity Hotspots of Africa

Table 2 Seven centers of endemism in Africa

 onservation and Sustainable Management of MAP Resources

The sustainable management of traditional medicinal plant resources has become an

Fig. 2 Endangered plant species in Africa (IndexMundi 2016)

A country-wise statistic of endangered species reveals that the African country

 aving Plants that Save Lives: Effect of Chiang Mai Declaration

Table 3 Spice and herb export (in US $) of 10 major exporting countries

Commercial Potential of African MAPs

Chemical and Pharmacological Potential of African MAPs

Cultivation of MAPs in Africa: A New Dimension in Agriculture

To-date, wild-crafting (collection) is still the main ways of harvesting medicinal

 ultivation in Farming Systems vs. Sustainable Exploitation

The Example of Prunus africana

Organic Production of MAPs in Africa

Growing international organic markets seem to stimulate organic production also in

MAPs in Traditional Medicine: A Heritage in Africa

 frican Herbal Pharmacopoeia and Research to Promote

medicinal plants. Despite the increasing popularity of phytomedicines of African

Keywords Aromatic plants • Central Africa • Chemical entities • Dietary supple-

Africa is a continent of tremendous biological and cultural diversity that have

B.-E. Van Wyk (*)

© Springer Science+Business Media B.V. 2017 19

Diversity of the African Flora

Popular and Commercially Relevant African Plant Species

(How.) Bern.Moens. and stimulant) D.R. Congo,

Engl. (Burseraceae) (fragrance)

Millsp.] (Euphorbiaceae) many other African

(Brassicaceae) headache, asthenia, Ethiopia

Myrtus communis L. Myrtle Leaves, fruits TM (expectorant, NA Egypt, Tunisia 5

Link (Fabaceae) tsotsorinangatra many other

L. (Fabaceae) cholesterol-lowering); TM Sudan 11

also topical for sores)

Fig. 1 Examples of African phytomedicines. 1, Centella asiatica leaves; 2, Griffonia simplicifolia

 ources of Information on African Medicinal and Aromatic

Conclusions and Future Prospects

Hedberg I, Staugård F (1989) Traditional medicinal plants: traditional medicine in Botswana.

Olufunke O. Fajinmi, Olaoluwa O. Olarewaju, and J. Van Staden

Keywords African culture • Traditional medicine • Aloe species • Wonder nut

1 Africa, Its People, Culture and Traditional Medicine

O.O. Fajinmi • J. Van Staden (*)

Médenine, Tunisia Mohamed Neffati

I ntroduction to Medicinal and Aromatic Plants in Africa........................... 1

ome Wild Herbaceous Plants of Nigeria: A Biological,

Africa as a Botanical Continent

Biodiversity Hotspots of Africa

onservation and Sustainable Management of MAP Resources

aving Plants that Save Lives: Effect of Chiang Mai Declaration

Commercial Potential of African MAPs

Chemical and Pharmacological Potential of African MAPs

Cultivation of MAPs in Africa: A New Dimension in Agriculture

ultivation in Farming Systems vs. Sustainable Exploitation

The Example of Prunus africana

Organic Production of MAPs in Africa

MAPs in Traditional Medicine: A Heritage in Africa

frican Herbal Pharmacopoeia and Research to Promote

Diversity of the African Flora

Popular and Commercially Relevant African Plant Species

ources of Information on African Medicinal and Aromatic

Conclusions and Future Prospects

1 Africa, Its People, Culture and Traditional Medicine

6 Legendary Plants from North Africa

Henna (Lawsonia inermis): Its Cultural, Traditional

ighly Utilized Medicinal Plants Across the Northern African

Marula, a Revered South African Native

calypha species Utilized in the African Traditional Medicine

edicinal Uses of Acalypha manniana, and A. wilkesiana

11 Progress Made so far by African Countries

3 Monographs, Pharmacopoeias, and Formularies

The Need for the Creation of Formularies

4 Some Selected Medicinal Plant Monographs

Catha edulis Forssk,

Catharanthus roseus (L.) G. Don

Corynanthe pachyceras K. Schum.

Erythrina abyssinica Lam ex DC,

Gymnema sylvestre R. Br,

Hypoxis hemerocallidea, Fisch, CA Mey & Ave-Lall.

Kigelia africana (Lam.) Benth,

Maytenus buchananii (Loes.) Wikzek.

Mondia whitei (Hook.f.) Skeels

Prunus Africana (Hook.f.) Kalkman,

Sclerocarya birrea (A. Rich.) Hochst.

Sutherlandia frutescens (L.) R.Br.

Tabernanthe iboga (L.) Nutt.

Toddalia asiatica (L.) Lam.

Voacanga africana Stapf,

2 Argan (Argania spinosa L.)

Chemical Composition of Argan-Oil

Acylglycerols, including 95% of triacylglycerols, constitute 99% of the Argan-

Medicinal Properties of Argan-Oil

3 Artemisia herba-alba Asso.

Chemical Composition of the Essential Oil

Use of A. herba-alba and Its Essential Oil

4 Atlas Cedar (Cedrus atlantica Mannerti)

Use of Atlas Cedar and Its Essential Oil

5 Laurel (Laurus nobilis and Laurus azorica)

Chemical Composition of Laurus Leaves

Chemical Composition of Laurus Berries