Wurzel 2016
Wurzel 2016
BACKGROUND: Protracted bacterial bronchitis (PBB) and bronchiectasis are distinct diag-
nostic entities that share common clinical and laboratory features. It is postulated, but re-
mains unproved, that PBB precedes a diagnosis of bronchiectasis in a subgroup of children.
In a cohort of children with PBB, our objectives were to (1) determine the medium-term risk
of bronchiectasis and (2) identify risk factors for bronchiectasis and recurrent episodes of
PBB.
METHODS: One hundred sixty-one children with PBB and 25 control subjects were pro-
spectively recruited to this cohort study. A subset of 106 children was followed for 2 years.
Flexible bronchoscopy, BAL, and basic immune function tests were performed. Chest CT was
undertaken if clinical features were suggestive of bronchiectasis.
RESULTS: Of 161 children with PBB (66% boys), 13 were diagnosed with bronchiectasis over
the study period (8.1%). Almost one-half with PBB (43.5%) had recurrent episodes (> 3/y).
Major risk factors for bronchiectasis included lower airway infection with Haemophilus
influenzae (recovered in BAL fluid) (P ¼ .013) and recurrent episodes of PBB (P ¼ .003). H
influenzae infection conferred a more than seven times higher risk of bronchiectasis (hazard
ratio, 7.55; 95% CI, 1.66-34.28; P ¼ .009) compared with no H influenzae infection. The
majority of isolates (82%) were nontypeable H influenzae. No risk factors for recurrent PBB
were identified.
CONCLUSIONS: PBB is associated with a future diagnosis of bronchiectasis in a subgroup of
children. Lower airway infection with H influenzae and recurrent PBB are significant pre-
dictors. Clinicians should be cognizant of the relationship between PBB and bronchiectasis,
and appropriate follow-up measures should be taken in those with risk factors.
CHEST 2016; 150(5):1101-1108
KEY WORDS: bacterial infection; bronchiectasis; pediatric lung disease; respiratory infection;
viral infection
ABBREVIATIONS: CFU = colony-forming units; IQR = interquartile Transplant Service (Dr Yerkovich), Prince Charles Hospital, Brisbane,
range; PBB = protracted bacterial bronchitis Australia; School of Medicine (Drs Yerkovich and Upham), The Uni-
AFFILIATIONS: From Queensland Children’s Medical Research Institute versity of Queensland, Brisbane, Australia; Child Health Division (Drs
(Drs Wurzel, Marchant, Petsky, Masters, Buntain, and Chang), Brisbane, Smith-Vaughan and Chang), Menzies School of Health Research,
Australia; Queensland Children’s Health Service (Drs Marchant, Petsky, Charles Darwin University, Darwin, Australia; and School of Medi-
Masters, and Chang), Brisbane, Australia; Murdoch Children’s Research cine (Dr Smith-Vaughan), Griffith University, Gold Coast, Australia.
Institute (Dr Wurzel), Melbourne, Australia; Queensland Lung
journal.publications.chestnet.org 1101
Protracted bacterial bronchitis (PBB), first described in appears to be associated with a future diagnosis of
2006, is a major cause of chronic cough in children.1,2 bronchiectasis.
PBB has been studied by research groups in Australia,1-3
Europe,4 and the United States, with similar findings.5,6 PBB and bronchiectasis share many common features,
PBB is characterized by persistent wet cough, response spanning from respiratory symptoms (ie, chronic
to 2 weeks of appropriate antibiotic therapy, and absence wet cough) to intense neutrophilic lower airway
of indicators to suggest an alternative cause for cough.1,7 inflammation and innate immune system activation.10-12
PBB is more common in young boys and children who Lower airway microbiota,13 including the presence
have attended child care.8 When compared with control of adenovirus type C14 and a predominance of
subjects, children with PBB are more likely to have lower non-typeable Haemophilus influenzae1,8 are also alike.
airway infection with common respiratory bacteria and These similarities underpin the notion that PBB and
viruses.8 bronchiectasis represent a clinical continuum.15,16 To
date, the accuracy of this proposed continuum is
Currently, there are limited published data9 and no uncertain and warrants evaluation in a prospective
prospective follow-up studies evaluating the outcomes of cohort study.
children with PBB. This research gap limits the
clinician’s ability to prognosticate on the likely natural Hence, in 161 children with PBB and 25 control
history of PBB in any given child. Anecdotally, many subjects, we aimed to determine (1) the 2-year outcomes
otherwise healthy children experience recurrent episodes of children with PBB with respect to a diagnosis of
of PBB without appreciable longer-term consequences. bronchiectasis and (2) risk factors for bronchiectasis and
However, in a subgroup of children, recurrent PBB recurrent episodes of PBB.
journal.publications.chestnet.org 1103
TABLE 1 ] Baseline Demographic and Clinical Characteristics of Study Participants
PBB Followed-up PBB total Control Subjects
Variable (n ¼ 106) (N ¼ 161) (n ¼ 25) P Valuea
Sex, Male (female) 74 (32) 106 (55) 12 (13) .085
Age, mo 23 (14-53) 22 (13-50) 44 (7-97) .061
Prior pneumonia radiographically 24 (23) 35 (22) 2 (8) .109
confirmed, No. (%)b
Household tobacco smoke 36 (34) 55 (34) 6 (24) .662
exposure, No. (%)
Aboriginal or Torres Strait 3 (3) 10 (6) 0 .363
Islander, No. (%)
Current cough, No. (%) 89 (84) 134 (83) 2 (8) < .001
Length of current cough, median 26 (7-52) 26 (6-52) 0 < .001
(IQR), wk
Current antibiotics, No. (%) 16 (15) 19 (12) 1/15 (7) .695
>5 doctor visits past y for cough, 95 (90) 140 (87) 3/15 (20) < .001
No. (%)
PBB followed-up refers to the subset of patients with PBB who completed 2-year follow-up; PBB total refers to all patients with PBB, irrespective of whether
2-year follow-up was completed. Boldface denotes statistical significance, P < .05. IQR ¼ interquartile range; PBB ¼ protracted bacterial bronchitis.
a
Comparison of “PBB total” to control subjects.
b
Parent-reported prior pneumonia.
Risk Factors for Bronchiectasis and Recurrent PBB respectively. Total cell counts, percentage of
Univariate analysis showed that bronchiectasis was macrophages, and percentage of lymphocytes were
significantly more likely to be diagnosed during similar between groups. Although the percentage of
follow-up when the child had recurrent PBB, when eosinophils in BAL fluid was slightly higher in those in
H influenzae infection was present (ie, cultured at a whom bronchiectasis developed (BAL eosin %, 1 [IQR,
clinically significant level of $ 104 CFU/mL in BAL 0-2) vs 0 [IQR, 0-0]; P ¼ .001), the median eosinophil
fluid), or in children with two or more siblings (Table 2). count was within the normal range (ie, < 2.5%),22 and
There were no significant intergroup differences for hence this finding was considered to be clinically
other factors. insignificant. Further, the difference was no longer
observed when the major outlier was removed.
Multivariate logistic regression showed that recurrent
PBB status and H influenzae infection were significantly In the examination of predictors for recurrent PBB,
associated with bronchiectasis diagnosis (OR, 11.48; univariate and multivariate analyses showed no
95% CI, 2.33-56.50; P ¼ .003 and OR, 7.60; 95% CI, significant difference between children with and those
1.53-37.79; P ¼ .013, respectively), whereas having two without recurrent PBB for the factors examined (sex,
or more siblings was no longer significant (OR, 3.53; age, prior pneumonia, tobacco smoke exposure,
95% CI, 0.98-12.70; P ¼ .054). Survival analysis, using maternal tobacco smoking in pregnancy, indigenous
Cox regression, concurred with logistic regression status, number of children in household, child care
findings. Participants with H influenzae lower airway attendance, and BAL fluid cellularity and
infection were more than seven times more likely to be microbiological characteristics) (e-Table 1). Notably,
diagnosed with bronchiectasis per month of follow-up H influenzae infection seen in BAL fluid did not predict
than were those without H influenzae infection, and recurrent PBB.
recurrent PBB status conferred more than a nine times
greater risk of bronchiectasis diagnosis per month H influenzae Typing
(Table 3).
Thirty-four of 55 H influenzae-positive samples (62%)
The percentage of lower airway neutrophils was similar were available for further characterization. Of these,
in children with and those without a subsequent H influenzae from the majority of samples (n ¼ 28
diagnosis of bronchiectasis (BAL neutrophil %, 35 [IQR, [82%]) were identified as non-typeable H influenzae;
12-75] vs 25 [IQR, 10-55]; P ¼ .35) in the group with encapsulated H influenzae were not identified.
bronchiectasis and the group without bronchiectasis, H influenzae from the remaining six samples were
Boldface denotes statistical significance, P < .05. PCR ¼ polymerase chain reaction. See Table 1 legend for expansion of other abbreviation.
a
As determined at 1-year time point in study. Seven children had not reached the 1-year time point. For the purposes of the multivariate analysis, these
children were assumed to have nonrecurrent PBB.
b
Compared with previously published rate of 4% (adenovirus positivity) in BAL samples from control children.8
c
b-lactamase-positive strains of H influenzae were found in 17 of 83 isolates (20.5%). Of these, 3 occurred in children from group 1 (27%) and 14 from
group 2 (19%); OR, 2.87; 95% CI, 0.70-11.67; P for difference between groups ¼ 0.143.
reassigned as H haemolyticus following species-specific recurrent (more than episodes 3 per year) PBB and the
polymerase chain reaction. presence of H influenzae infection in the lower airways.
Further, H influenzae infection, compared with no
infection, conferred more than a seven times higher risk
Discussion
of a bronchiectasis diagnosis.
This is the first prospective longitudinal cohort study of
children with PBB. In our cohort, which was based in a Findings from this study, the first to investigate the
large tertiary pediatric hospital, almost 44% of subjects 2-year outcomes of children with PBB, support the
had recurrent episodes (more than three episodes in the hypothesis that PBB and bronchiectasis represent a
first year after recruitment) and approximately one in 12 clinical spectrum. Children with PBB have
were diagnosed with bronchiectasis at 2 years. We endobronchial bacterial infection and neutrophilic
identified two significant risk factors for bronchiectasis: airway inflammation, factors known to be injurious to
journal.publications.chestnet.org 1105
TABLE 3 ] Survival Analysis: PBB to Bronchiectasis In this study, bronchiectasis was diagnosed at an early age
(mo) (N ¼ 161) (median, 38 months), similar to our other cohorts of
HR (95% CI) P Value children with bronchiectasis.10,20,35 Together with findings
Univariate from earlier studies on PBB,8,14 this observation lends
H influenzaea 5.81 (1.28-26.37) .022 further support to the assertion that timing of airway
$ 2 siblings 3.18 (1.04-9.73) .042 infection (with bacteria or viruses, or both), with respect to
Recurrent PBB 7.65 (1.67-34.92) .009 age, is likely to be important.14 All children had mild or
(> 3 episodes/y) early cylindrical bronchiectasis, which is potentially
Multivariate reversible if treated early and intensively,36-38 prior to the
H influenzaea 7.55 (1.66-34.28) .009 development of severe (saccular or cystic) bronchiectasis.
Recurrent PBB 9.77 (2.13-44.80) .003
(> 3 episodes/y) In support of previous studies on PBB, the present study
showed that PBB is characterized by active lower airway
Boldface denotes statistical significance, P < .05. See Table 1 legend for
neutrophilic inflammation. Although the median
expansion of other abbreviation.
a
Lower airway infection with H influenzae defined as $ 104 CFU/mL neutrophil percentage in BAL fluid was greater in
growth on BAL fluid culture. children with PBB who were subsequently diagnosed
with bronchiectasis compared with those who were not
(median, 35% vs 25%, respectively), this difference was
the airways.23 Our findings suggest that in a subset of
not significant. This suggested that the degree of airway
children receiving close follow-up by pediatric
neutrophilia did not predict bronchiectasis. Regarding
pulmonologists, recurrent episodes (more than three per
the intergroup disparity in airway eosinophil levels
year) of PBB precede a diagnosis of bronchiectasis.
between those with and those without bronchiectasis,
Further, although a cause-effect relationship cannot be
this is unlikely to be of clinical relevance for two reasons.
concluded, an association between H influenzae
First, eosinophil levels in both groups were within
infection and bronchiectasis has been shown. This
normal reported ranges and second, when the major
finding is in accordance with the increasing recognition
outlier was removed (16% eosinophils in a child with
of the role of H influenzae in the pathogenesis of chronic
features consistent with persistent asthma in addition to
respiratory diseases.24
PBB), the difference was no longer significant.
H influenzae is the most common bacterial species
Several limitations to this study merit discussion. The
infecting the lower airways of children with
most significant limitation is the fact that we did not
endobronchial suppuration, including PBB,8 recurrent
perform CT imaging in all children at study entry and
or nonresponsive community-acquired pneumonia,25
exit. Given the potential increased lifetime risk of cancer
and bronchiectasis.8,10,26 H influenzae is also the major
associated with exposure to CT imaging (and the
bacterial pathogen associated with chronic respiratory
relatively common nature of PBB in otherwise well
disorders in adults, for example, bronchiectasis and
children),39 we could not justify performing two CT
COPD.24,27,28 Although H influenzae infection may be
scans on every child.18 The impact of this limitation is
an important risk factor for bronchiectasis,26,29-32 there
twofold. First, bronchiectasis may have been missed in
are likely to be other contributory factors.
some children at recruitment and indeed at follow-up.
Our earlier studies identified that children with PBB Hence, we cannot conclude that PBB progresses to
were significantly more likely than control subjects to bronchiectasis per se. Rather, our findings indicate that
have attended child care and to have viral infection of in children with PBB (who are followed closely by
their lower airways, particularly with adenovirus type pulmonologists), a proportion (approximately 8%) will
C.8,14 It is plausible, although beyond the scope of this be diagnosed with bronchiectasis on chest CT imaging at
study, that coinfection with specific pathogens—for 2 years. Second, we acknowledge the fact that
example, adenovirus C and H influenzae—33 initiates the performing CT imaging in only a select subgroup
vicious cycle of lower airway infection and inflammation introduces the possibility of selection bias in that
inherent to these conditions.34 Factors such as young age individual clinicians may have different indications for
at initial infection (and concomitant immune and performing chest CT imaging. To address this, we
respiratory system immaturity) may also play a key role compared children with PBB undergoing chest CT
in the etiopathogenesis.14 imaging with those who did not and found no
journal.publications.chestnet.org 1107
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