The document provides instructions for instrument settings and reagent preparation for analyzing urea or BUN levels using an enzymatic spectrophotometric method on AU 400/640 instruments. It specifies parameters like sample volume, reagent volumes, wavelengths, measuring points, calibration type and process.
The document provides instructions for instrument settings and reagent preparation for analyzing urea or BUN levels using an enzymatic spectrophotometric method on AU 400/640 instruments. It specifies parameters like sample volume, reagent volumes, wavelengths, measuring points, calibration type and process.
The document provides instructions for instrument settings and reagent preparation for analyzing urea or BUN levels using an enzymatic spectrophotometric method on AU 400/640 instruments. It specifies parameters like sample volume, reagent volumes, wavelengths, measuring points, calibration type and process.
The document provides instructions for instrument settings and reagent preparation for analyzing urea or BUN levels using an enzymatic spectrophotometric method on AU 400/640 instruments. It specifies parameters like sample volume, reagent volumes, wavelengths, measuring points, calibration type and process.
The application parameters comprised here constitute a guide to facilitate the validation of our reagents by the instrument. It is advisable to validate the use when there is any change in software or reagent versions.
Instrument: AU 400 / 640
Reagent preparation Working Reagent: Transfer the contents of one Reagent B vial into a Reagent A bottle. Stable for 2 months at 2-8 ºC.
Instrument settings
Specific Test Parameters Min OD Max OD
Sample: Volume 2 µL Dilution 0 µL L -0.1 H 2.5 Reagents: R1 Volume 300 µL Dilution 0 µL Reagent OD limit: Wavelength: Pri. 340 Sec. 380 First L -0.1 First H 0.5 Method: FIX Last L -0.1 Last H 0.5 Reaction slope: - Dynamic Range: Measuring Point 1: First 2 Last 5 L 0 H 300 Measuring Point 2: First 0 Last 0 Correlation Factor: Linearity: % A 1 B 0 No Lag Time: On-board stability period:
L H 7. None selected 8. Out of range 10 50 L H Panic Value Unit: mg/dL Decimal places: 0
Calibration Specific
Calibration Type: AB Formula: Y=AX+B Counts: 2 Process: CONC.
