This document provides information about a lesson on chromatography. The lesson will be conducted over two 1-hour sessions for a 10th grade science class. Students will learn about chromatography by grinding green leaves and performing a paper chromatography experiment to separate and identify different pigments present in leaves besides chlorophyll. Through hands-on exploration and explanation of their observations, students will understand that other pigments exist in leaves and be able to apply the process of chromatography.
This document provides information about a lesson on chromatography. The lesson will be conducted over two 1-hour sessions for a 10th grade science class. Students will learn about chromatography by grinding green leaves and performing a paper chromatography experiment to separate and identify different pigments present in leaves besides chlorophyll. Through hands-on exploration and explanation of their observations, students will understand that other pigments exist in leaves and be able to apply the process of chromatography.
This document provides information about a lesson on chromatography. The lesson will be conducted over two 1-hour sessions for a 10th grade science class. Students will learn about chromatography by grinding green leaves and performing a paper chromatography experiment to separate and identify different pigments present in leaves besides chlorophyll. Through hands-on exploration and explanation of their observations, students will understand that other pigments exist in leaves and be able to apply the process of chromatography.
This document provides information about a lesson on chromatography. The lesson will be conducted over two 1-hour sessions for a 10th grade science class. Students will learn about chromatography by grinding green leaves and performing a paper chromatography experiment to separate and identify different pigments present in leaves besides chlorophyll. Through hands-on exploration and explanation of their observations, students will understand that other pigments exist in leaves and be able to apply the process of chromatography.
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Unit title Separation Technique
Lesson topic Chromatography
Sub-topic Experiment # 3 Teacher Ms. J. Gavin Date Jan, 2019. Grade/Class 10 TAR Duration 2 Session (1hr)- 9:15-10:15 General Objective Apply suitable separation technique based on differences in properties of the components of mixtures Scientific Skills Teaching Hands-on Inquiry based (Practical Work), Questioning and Audio Methodology Visual Technological resources. Prior Knowledge Before completing this lesson, student should have a basic understanding of the following standards: • Types of mixtures • Miscible and Immiscible liquids • Definition of solubility Specific Lesson At the end of this review session, students should be able to: Objectives i. apply process skills in understanding chromatography ii. Understand that pigments other than chlorophyll are present in leaves. Materials Ruler, Green leaves, Mortar and pestle for grinding leaves, Engage Students will be asked why they think leaves are green. Students will be asked whether they think other pigments besides chlorophyll exist in leaves. Why do they think so? How can they demonstrate this?
Students will then watch a short video on how to carry out paper chromatography experiment. Students will follow the instructions below to explore what pigments Explore exist in leaves. METHOD: 1. Draw a horizontal line 2-3 cm3 from the bottom of the chromatography paper 2. Mark an ‘X’ in the center of the line 3. Using a scissors cut Coral Vines into fine pieces into mortar. 4. Add 5ml of acetone into the mortar. 5. Grind coral vines using mortar and pestle 6. Put a drop of the leave extract in the middle of the line on the filter paper. 7. Add 2 cm3of acetone into the beaker 8. Carefully insert the chromatography paper into the beaker with acetone. (about 5 mm height) 9. Make observations and calculate the Rf values for the different components of chlorophyll.
Explain students will write and draw in their science lab book about: • What their observations are from the beginning, middle and end of the experiment. (What happened when the acetone/groundup-leaves mixture traveled up the filter paper?), what colour pigments observed
DIAGRAMS: Before - (draw a copy of your spotted chromatogram) After – (paste your dried chromatogram at the end of the experiment)
Evaluate Discussion Lab Report
What is chromatography? Identify the mobile and stationary phases in this lab Explain what the Rf values tell you about each component
• Why they think what they observed occurred.
• Ask students to infer what pigment molecules might be present in the leaves based on the colors on the strip. Extend
