Gene Expression

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GENE EXPRESSION

The process of turning on a gene to produce RNA and protein


- Regulation of gene expression conserve energy and space
- DNA must be unwound from its tightly coiled structure to transcribe and translate
the DNA
- Gene regulation is the process of controlling which genes in a cell’s DNA are
expressed (used to make a functional product such as a protein).
- Different cells in a multicellular organism may express very different sets of
genes, even though they contain the same DNA.
- The set of genes expressed in a cell determines the set of proteins and functional
RNAs it contains, giving it its unique properties.
- In eukaryotes like humans, gene expression involves many steps, and gene
regulation can occur at any of these steps. However, many genes are regulated
primarily at the level of transcription.
PROKARYOTIC
- To synthesis protein= transcription and translation occur almost
simultaneously
- the primary method to control what type of protein and how much of each
protein is expressed in a prokaryotic cell is the regulation of DNA
transcription.
- the control of gene expression is mostly at the transcriptional level.

EUKARYOTIC
- DNA is contained inside the cell’s nucleus and there it is
transcribed into RNA
- newly synthesized RNA is then transported out of the nucleus into
the cytoplasm, where ribosomes translate the RNA into protein
- processes of transcription and translation; separated by the
nuclear membrane
- transcription occurs only within the nucleus
- transcription occurs only within the nucleus
epigenetic level: DNA is uncoiled and loosened from
nucleosomes to bind transcription factors
transcriptional level: when the RNA is transcribed
post-transcriptional level: when the RNA is processed and
exported to the cytoplasm after it is transcribed
translational level: when the RNA is translated into protein

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