Green Certification of MPEDA

ISBN 978-81-910104-0-4
Phone: +91-484-2311979 Fax: +91-484-2312812

Kochi – 682 036, Kerala, India.
P.B.No. 4272, MPEDA House, Panampilly Nagar,
(Ministry of Commerce & Industry, Government of India)
THE MARINE PRODUCTS EXPORT DEVELOPMENT AUTHORITY
cmyk
gripper

Phone: +91-484-2311979 Fax: +91-484-2312812
ISBN 978-81-910104-0-4
gripper
cmyk
Phone: +91-484-2311979 Fax: +91-484-2312812
ISBN 978-81-910104-0-4
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GUIDELINES FOR
GREEN CERTIFICATION OF
FRESHWATER ORNAMENTAL FISH
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Phone: +91-484-2311979 Fax: +91-484-2312812
Citation: Silas, E.G., Gopalakrishnan, A., Ramachandran, A., Anna Mercy, T.V., Kripan Sarkar,
Pushpangadan, K.R., Anil Kumar, P., Ram Mohan, M.K. & Anikuttan, K.K. (2011).
Guidelines for Green Certification of Freshwater Ornamental Fish. The Marine Products
Export Development Authority, Kochi, India. xii + 106 p.
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© 2011, The Marine Products Export Development Authority

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Published by:
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Director
The Marine Products Export Development Authority
Ministry of Commerce and Industry, Govt. of India,
Panampilly Nagar, Kochi - 682 036, Kerala, India.
Tel : +91-484-2311979
Fax: +91-484-2313361
E-mail: [email protected]
Web: http://www.mpeda.com
ISBN 978-81-910104-0-4
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CONTENTS
Foreword ---------------------------------------------------------------------------------------------------- v
Acknowledgements --------------------------------------------------------------------------------------------------- vi
Preface -------------------------------------------------------------------------------------------------- vii
Vision and Mission --------------------------------------------------------------------------------------------------- ix
Executive Summary --------------------------------------------------------------------------------------------------- x
Introduction --------------------------------------------------------------------------------------------------- 1
Global Facts --------------------------------------------------------------------------------------------------- 1
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Indian Scenario ------------------------------------------------------------------------------------------------ 2
Objective --------------------------------------------------------------------------------------------------- 3
Task Force --------------------------------------------------------------------------------------------------- 3
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Protocols and Procedures ---------------------------------------------------------------------------------------- 5
1. Collection of Ornamental Fishes and Invertebrates from the Natural Water Bodies -------- 5
2. Primary Holding Facility ------------------------------------------------------------------------------------- 6
3.
4.
5.
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Secondary/ Main Holding Facility ------------------------------------------------------------------------- 6
Exporting Facility ---------------------------------------------------------------------------------------------- 7
Culture Facility ------------------------------------------------------------------------------------------------- 7
5.1. General requirements ------------------------------------------------------------------------------- 7
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5.2. Mandatory building facilities to be constructed ----------------------------------------------- 7
5.3. Site selection ------------------------------------------------------------------------------------------ 7
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5.4. Source of water --------------------------------------------------------------------------------------- 8

5.5. Infrastructure required ------------------------------------------------------------------------------ 8
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5.5.1. Fish ponds ------------------------------------------------------------------------------------- 9

5.5.2. Fish tanks -------------------------------------------------------------------------------------- 9
5.5.3. Reservoir tanks and overhead tanks -------------------------------------------------- 10
5.5.4. Supply and drainage system ------------------------------------------------------------ 10
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5.5.5. Quarantine cum conditioning area ----------------------------------------------------- 10

5.5.6. Fish health monitoring and management section ---------------------------------- 11
5.5.7. Fish feed preparation / storage area -------------------------------------------------- 12
5.5.8. Fish holding area --------------------------------------------------------------------------- 12
5.5.9. Packing area --------------------------------------------------------------------------------- 13
5.5.10.Water and air supply equipments ------------------------------------------------------ 13
5.5.11.Electricity / Generator / Fuel store ----------------------------------------------------- 13
5.5.12.Sedimentation pond ----------------------------------------------------------------------- 13
5.5.13.Effluent water treatment plant ---------------------------------------------------------- 14
5.5.14.Office and Accommodation / Rest room --------------------------------------------- 14
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6. Environmental Integrity ------------------------------------------------------------------------------------- 14
7. Best Management Practices (BMP) -------------------------------------------------------------------- 15
8. Green Seal Labelling --------------------------------------------------------------------------------------- 15
9. Documentation & Maintenance of Data ---------------------------------------------------------------- 17
10. Agencies for Accreditation and Verification ----------------------------------------------------------- 17
11. Programme Implementation ------------------------------------------------------------------------------ 17
11.1. Short Term Plans ------------------------------------------------------------------------------------ 17
11.2. Long Term Plans ------------------------------------------------------------------------------------ 17
12. Standards for Assessment -------------------------------------------------------------------------------- 17
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13. Import of Exotic Fishes ------------------------------------------------------------------------------------ 18
14. Geographical Indication of Species --------------------------------------------------------------------- 18
15. Conclusion -------------------------------------------------------------------------------------------------- 25
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16. Annexures
16.1. Annexure 1- Model Design for Ornamental Fish Primary Holding Facility ----------- 26
16.2. Annexure 2 - Model Design for Ornamental Fish Secondary Holding Facility ------ 26
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16.3. Annexure 3 - List of Medicines/ Antibiotics for Treating Diseases --------------------- 27
16.4. Annexure 4 - Model Design for Ornamental Fish Export Facility ----------------------- 28
16.5. Annexure 5 - Model Design for Ornamental Fish Culture Cum Trading Facility ---- 28
16.6. Annexure 6 - Disinfection & Disposal Methods --------------------------------------------- 29
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16.7. Annexure 7 - Dispatch of Sick Fishes and Representative Samples for
Further Examination -------------------------------------------------------------------------------- 33
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16.8. Annexure 8 - Biopsy Techniques --------------------------------------------------------------- 34

16.9. Annexure 9 - List of items for diseased and moribund fish receiving and
autopsy and biopsy section ----------------------------------------------------------------------- 39
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16.10.Annexure 10 - List of items for Water quality analysis section -------------------------- 39

16.11.Annexure 11 - Documentation and Maintenance of Data -------------------------------- 40
16.12.Annexure 11 A - Log Book Format - Collection/Procurement --------------------------- 43
16.13.Annexure 11 B - Log Book Format - Primary Holding Facility --------------------------- 45
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16.14.Annexure 11 C - Log Book Format - Secondary Holding Facility ---------------------- 47

16.15.Annexure 11 D - Log Book Format - Exporting Facility ----------------------------------- 49
16.16.Annexure 11 E - Log Book Format - Breeding & Culture Facility ----------------------- 52
16.17.Annexure 12 A to 12 E - Score cards for Assessment of the facilities ----------- 58-72
16.18.Annexure 13 - Guidelines for the Import of Ornamental Fishes to India -------------- 76
16.19.Annexure 14 - OIE Listed Diseases ------------------------------------------------------------ 97
16.20.Annexure 15 - NACA list of Diseases in Asia Pacific -------------------------------------- 98
16.21.Annexure 16 - Images of Freshwater Ornamental Fish Species of
India suggested for GI ----------------------------------------------------------------------------- 99
17. Glossary ------------------------------------------------------------------------------------------------ 106
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FOREWORD
Keeping colourful and fancy fishes known as ornamental fishes, aquarium fishes, or live jew-
els is one of the oldest and most popular hobbies in the world. The growing interest in aquarium
fishes has resulted in steady increase in aquarium fish trade globally. The ornamental fish trade
with a turnover of US $ 6 Billion and an annual growth rate of 8 percent offers lot of scope for
development. Unfortunately India’s share in ornamental fish trade is estimated to be less than 1 %
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of the global trade. The major part of the export trade is based on wild collection. There is very
good domestic market too, which is mainly based on domestically bred exotic species.
The earning potential of this industry and the relatively simple techniques involved in the
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growing of these fish has helped the aquarium industry to evolve and provide rural employment
and subsequent economic upliftment in many countries including India. Ornamental fish trade has
much more to off er than what is exploited now as far as India is concerned. As the trade expands,
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issues such as quality, environmental concerns, habitat protection and sustainability of the re-
source come to the fore simultaneously. For the trade to prosper, such issues have to be ad-
dressed giving due importance. The recent release of Global Guidelines for Aquaculture Certifica-
tion in October 2010 by the Subcommittee on Aquaculture under the Committee on Fisheries of
FAO, point out similar certification requirements in future in other sectors also. This could be
considered as an indication of the future requirements the ornamental fish trade would require to
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comply with. We at MPEDA felt a similar effort covering all the concerns above would be benefi-
cial for the ornamental fish production sector in India in gaining more market appreciation.
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Green certification is the certification given to a product to ensure its environmental and socio-
economic sustainability. It ensures product quality, safety and traceability. Keeping in line with
these concepts, MPEDA, in association with UNCTAD and Project PIABA, Brazil organized an
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international workshop on Green certification of ornamental fishes in October 2008, which was the
first of its kind in the sector. A national task force was constituted after the workshop to prepare the
guidelines for green certification of freshwater ornamental fishes traded within and exported from
India. The guidelines in the present form have been prepared through various deliberations and
after incorporating the comments from organizations who have offered their valuable suggestions
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/ comments on the draft.
I congratulate the Task Force members as well as all those who have put in their efforts to
bring out this beautiful publication. I am also grateful the various organizations and experts for
their constructive suggestions and comments to make this venture meaningful. I am sure that this
will surely give an impetus to the growth and export of the ornamental fish sector of India.
LEENA NAIR
CHAIRMAN
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ACKNOWLEDGEMENTS
MPEDA acknowledges the efforts put in by the following members of the Task Force for
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bringing out this guidelines:
1. Dr. E.G. Silas, Former Vice Chancellor, Kerala Agricultural University, Kochi
2. Dr. A. Ramachandran, Professor, School of Industrial Fisheries, Cochin University of Science
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& Technlogy, Kochi
3. Dr. A. Gopalakrishnan, Principal Scientist, NBFGR Unit, Kochi
4.
5.
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Dr. T.V. Anna Mercy, Professor, College of Fisheries, Kerala Agricultural University, Ernakulam
Mr. K.R. Pushpangadhan, Ornamental fish Exporter, Kerala
6. Mr. Kripan Sarkar, Ornamental fish breeder, West Bengal

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7. Mr. Anilkumar P., Technical Officer, INFOFISH, Malaysia
Mr. G. Mohan Kumar, IAS, former Chairman, MPEDA was a source of inspiration and inces-
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sant support for organizing the international workshop on green certification as well as for the
follow up activities during his tenure at MPEDA.
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Thanks are also due to Mr. Kuruvilla Thomas, IFS, former Director (Marketing), MPEDA for
the contributions he has made during his tenure at MPEDA for the successful culmination of the
envisaged objectives.
We acknowledge the valuable comments and suggestions received from OFI (Ornamental
Fish International), The Netherlands, WWF (World Wide Fund for nature), INFOFISH, Malaysia,
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CMFRI (Central Marine Fisheries Research Institute), Kochi, CIFA (Central Institute of Freshwater
Aquaculture), Bhubaneswar and Assam Agricultural University, Assam.
The inputs from Dr.I.S. Bright Singh, Cochin University of Science and Technology, Dr. V.S.
Basheer and Dr. T. Raja Swaminathan of National Bureau of Fish Genetic Resources (NBFGR),
Cochin Unit for bringing out this guidelines are also duly acknowledged.
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PREFACE
The potential for the development of ornamental fish trade in India is immense, though it is still
in a nascent stage. The Government of India has identified this sector as one of the thrust areas
for development to augment exports. The turn of the century has seen a spurt in the collection,
culture and trade in freshwater ornamental fishes. Aqua-shows have now become an annual fea-
ture in some states as in Kerala where Government support for such an activity is in vogue. For the
trade to prosper, the three pre requisites are quality, quantity and sustainability. The fish species
diversity of the rivers and streams of the Western Ghats and North East India are well recognized
with as many as 68 per cent of the 327 species listed from the former and over 50 per cent of the
350 or so listed from North East India being endemics. Of these, 40 to 50 per cent are ornamen-
tals, some fetching very high prices in the international market. Ninety per cent of the freshwater
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ornamental fishes exported from India are wild caught indigenous species. The total marine prod-
ucts exported from India in 2009-2010 was about Rs.100485 million (US $ @ 2132 million) of
which ornamental fish formed only a minuscule of hardly Rs.55 million (US $ @ 1.17 million).
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We are today in an unenviable position, so that we could consider policies on environmental,
and human management approaches to make the growing industry of freshwater ornamental fish
trade sustainable, eco-friendly and at the same time monitor resilience of the resources. We have
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to create an awareness among local communities and stakeholders to desist from unlawful and
illegal practices of catching ornamental fishes from the wild. The trade should also encourage
protection of the habitat for an eco-friendly approach. Presently exotic species dominate our do-
mestic aquarium trade, and some are being bred for export. Unregulated/illegal introduction of
exotic species and control or elimination of invasive species pose problems and these issues have
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to be addressed.
It is in this context that the initiative taken by the Marine Products Export Development Au-
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thority (MPEDA) in organizing an “International Workshop on Green Certification of Ornamental

Fish” in association with UNCTAD and PROJECT PIABA, Brazil assumes great importance. This
workshop held at Cochin, India from 14 to 18 October 2008 was a milestone in this direction. It
stressed the need for developing a value chain approach from collection/culture to export of orna-
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mental freshwater fishes. Unfortunately, to date, we do not have any proper guidelines or code of
practices for the freshwater ornamental fishes similar to marine ornamental fishes developed by
Marine Stewardship Council (MSC) and the Marine Aquarium Council (MAC). Just as the Forest
Stewardship Council (FSC) and the MSC, it is high time to develop a “Freshwater Stewardship
Council (FSC)” as well as a “Freshwater Aquarium Council (FAC). In Brazil, it is the Forest Stew-
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ardship Council that handles certification of Cardinal tetra caught from the wild by traditional means
and this has the approval of organizations such as the World Conservation Union, World Wild life
Fund (WWF) and TRAFFIC.
Thus, the International Workshop at Cochin had opened up new vistas for developing mo-
dalities for Green Certification for Freshwater Ornamental fishes. Equally significant is the fact that
in December 1999, the Indian Parliament passed the Geographical Indication of Goods (Registra-
tion and Protection) Act 1999 which seeks to provide for the Registration and Protection of Geo-
graphical Indications relating to goods in India. In the case of ornamental fish, Geographical Indi-
cation becomes imperative as it will indicate the natural distribution of a species and the location /
country it belongs to. This will give legal protection to the concerned species and help in the event
of any IPR / Patent issues, as the natural distribution of the species alone will have to be taken into
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consideration and not the places / regions they have been introduced / transplanted through ex-
port or other man-made activities.
A major recommendation of the Cochin International Workshop which was attended by del-
egates from India and abroad was that under the auspices of MPEDA a “Task Force” be consti-
tuted to develop the Guidelines, Regulations and Code of Practices for Green Certification of
Freshwater Ornamental Fishes and their Geographical Indications (GI). The animal health, com-
munity/ stakeholders’ welfare and the socio-economic sustainability are also matters of concern
to be addressed. Accordingly, a seven member Task Force was constituted. The Task Force had
nine sittings and went in depth into the various facets of details encompassing Green Certification
of Freshwater Ornamental Fishes. The report of the Task Force was sent to various national and
international organizations for comments and suggestions. The suggestions on the report received
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from OFI, WWF, INFOFISH, CIFA, CMFRI were analysed and relevant ones have been incorpo-
rated after a series of deliberations.
The following aspects such as collection from the wild, handling, transport, holding, breeding
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and culture facilities, conditioning for export, infrastructure and maintenance of records to conform
to a value chain system for delivering healthy ornamental fishes to the trade and the hobbyist have
been taken into consideration. The guidelines also addresses the Chain of Custody verification
methods as proposed by the FAO to cover the tracking/ traceability of the product all along the
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collection, production, transportation, holding, breeding and marketing chain. This also encom-
passes traceability and transparency. The adoption of the Report and its implementation will go a
long way to maintain the highest standards in the trade of freshwater ornamental fishes from India.
The Certification should also assure enhanced economic returns, reduction in risks including mor-
tality and reduction in the cost of production. Green Certification should not be considered as a
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threat, but as a facilitator for excellence in quality, standards and ethics.
Green Certification may be introduced on a voluntary basis in a phased manner once the
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implementing agencies are identified and training programmes organized for the stake holders
and entrepreneurs for adopting the system. However, for wild caught native ornamental fishes,
Green Certifcation may be made mandatory with a transition period of three years from the date of
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implementation of the guidelines.
E.G. SILAS
CHAIRMAN (Task Force)
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VISION & MISSION
Vision
“Conservation and sustainable development of freshwater ornamental fish sector involving

collection, farming and marketing through a value chain system enabling certification at all stages.”
Mission
i) Create societal awareness about the significance of green certification in conserving and
sustaining freshwater ornamental fish resources and their habitats.
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ii) Manage collection area according to the principles of ecosystem management ensuring eco-
system integrity and the sustainable use of the ornamental fish resources.
iii) Gradually reduce the dependence on the wild stocks, to maintain the genetic diversity, by
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promoting captive breeding and farming practices.
iv) Promote closed seasons, protected areas including sanctuaries and natural spawning grounds.
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Adopt eco-friendly practices of collection, farming, handling, transportation of freshwater or-
namental fishes including eggs and larvae and marketing of freshwater ornamental fishes
vi) Stop the release of farmed/alien species to the natural water bodies for safe guarding the
genetic integrity and susceptibility to diseases of the native stocks.
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vii) Establish proper documentation which is mandatory at all stages (from collection/farming to
marketing).
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viii) Establish Green Certification system to achieve sustainability and social security, reduce risk
and cost, and to enhance income.
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EXECUTIVE SUMMARY
India, blessed with the richness of its biodiversity and strategic geographical proximity and
connectivity to the international markets can capitalize on these two factors to carve out a promi-
nent place in the global trade of ornamental fishes. For achieving this ambitious target, efforts
must be channeled to strengthen the infrastructure, knowledge base and marketing links with an
eco friendly and sustainable approach ensuring socially acceptable and economically viable de-
velopment of the sector.
The success of any product in the market is its consumer acceptance and assurance of cer-
tain standards to it by a certification system adds value to the product and enhances its consumer
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preference. In this context the concept of Green Certification assumes importance as it takes care
of the environmental concerns as well as ensures a value chain system linking all segments of the
sector from collector / producer to the consumer. In line with these objectives, the MPEDA, in
collaboration with UNCTAD and Project PIABA, Brazil organized an international workshop on
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Green Certification of ornamental fishes from 14 to 18 October 2008 at Kochi,India. As per the
recommendation of the Workshop, a seven member national level “Task Force” was constituted
under the auspices of MPEDA to develop the guidelines, regulations and code of practices for
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Green Certification of freshwater ornamental fishes and their Geographical Indication (GI). The
Task Force, after serious brainstorming sessions and deliberations has prepared the guidelines
incorporating the following aspects such as collection from the wild, handling, transport, holding,
breeding and culture facilities, conditioning for export, infrastructure and maintenance of records
to conform to a value chain system for delivering healthy ornamental fishes to the trade and the
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hobbyist.
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The gist of the various topics can be summarized as follows:
1. Guidelines for Collection of Ornamental fishes and invertebrates from natural water bodies:
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❖ This section covers the aspects such as nature & type of water body from which fish is
collected, the methods for collection, handling and transportation.
2. Guidelines for Primary Holding Facility
❖
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The various aspects described include items such as site selection, water source, treat-
ment of discharge water, and transport of the fishes.
3. Guidelines for Secondary Holding Facility
❖ Major areas covered includes site selection, type of tanks to be used, Best Management
Practices (BMP’s), laboratory, acclimatization, packaging, transport, use of antibiotics,
water treatment and disposal of dead fish.
4. Guidelines for Exporting Facility
❖ This section addresses areas such as source of the fishes, quarantine measures, BMPs,
Health certificates and buyer requirements.
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5. Guidelines for Culture Facility
❖ The major aspects covered includes site selection, source of water, BMPs, use of or-
ganic manure, mandatory building/infrastructure facilities, requirements for ponds and
tanks, supply and drainage system, water treatment, quarantine and health manage-
ment, fish holding area, packing area, water and air supply requirements
6. Environmental Integrity
❖ This section addresses the significance of environmental concerns which have to be

considered while evolving management strategies and protocols at various segments of
the value chain. Major aspects mentioned include responsible use of various energy
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sources, feed, feed additives, chemicals, drugs and antibiotics.
7. Best Management Practices (BMP)
❖ The most ideal practices to be adopted at different facilities for achieving the overall
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objectives of green certification are described under this section. The areas covered
includes live feed culture, feeding and feed preparation, use of chemicals for modifying
the natural colour of the fishes, post mortem and disposal of dead fish, handling of fishes
8. Green Seal Labelling (Eco Labelling)
❖
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and use of tranquilizers / anesthetics.
Green Seal labelling procedure has to be adopted to indicate that the origin of the fish is
from a certified operation and chain of custody (CoC) from buyer to consumer. Green
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seal certification requires that the ornamental fish in the CoC passes through the estab-
lished processes and standards of fish catching, handling, culturing, conditioning, pack-
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ing and marketing. The Green Seal is also concerned with environmentally responsible,
socially acceptable and economically viable procedure of production and marketing of
ornamentals
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9. Documentation & Maintenance of Data
All the information has to be recorded and maintained at every facility/ section in the value
chain so as to ensure the effective monitoring and surveillance of the system. This information
is described in detail under this section. The format for log books and registers to be main-
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tained at each section is given as annexures to this chapter.
10. Agencies for Accreditation and Verification
❖ Accreditation Agency and Verification Agency should be constituted by MPEDA which

will be the Nodal Agency for implementation of Green Certification System in the country.
11. Programme implementation. The procedure for implementation of the programme is described
under this section, which includes short term and long term plans.
12. Standards for assessment. The standards on which the assessment of the facilities is to be
done is described here. Score cards for each facility has been prepared and is given as
annexures.
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13. Import of exotic Fishes
“Guidelines for the Import of Ornamental Fishes into India” issued by Ministry of Agriculture
based on the recommendations of National Committee on Introduction of Exotic Aquatic Spe-
cies into India must be followed for the import of exotic fishes to India. The relevant informa-
tion on this topic is also included as annexure to this chapter.
14. Geographical Indication of species
❖ In December 1999, the Indian Parliament passed the Geographical Indication (GI) of
Goods (Registration and Protection) Act 1999 which seeks to provide for the Registration
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and Protection of Geographical Indications relating to goods in India. In the case of orna-
mental fish, Geographical Indication becomes imperative as it will indicate the natural
distribution of a species and the location / country it belongs to. The registration of a GI
may act as a certification that the product possesses certain qualities, or enjoys a certain
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reputation, due to its geographical origin such as Puntius denisonii (Day), from the West-
ern Ghats, India.
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Training programmes will have to be organized for the stakeholders and the personnel of the
implementing agencies by MPEDA to familiarize the guidelines and to create an awareness for the
need to supply healthy ornamental fishes to the aquarium hobbyists, captured or cultured in an
eco-friendly and sustainable manner. The stakeholders, breeders and exporters must be self-
motivated to implement the green certification guidelines and the aquarium hobbyists may prefer
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buying the certified specimens, as the green certification process is aimed at producing quality
fishes, reducing mortality at all stages, fetching far better prices, avoiding unethical practices and
making all links of the chain sustainable and eco-friendly.
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Guidelines for Green Certification of Freshwater Ornamental Fish
INTRODUCTION
Green certification is the certification given to a product to ensure its environmental and socio-
economic sustainability. It is a procedure by which a third party gives written or equivalent assur-
ance that the operation confirms to the relevant standard in relation to social issues, environmen-
tal impacts on animal (fish) welfare. It ensures product quality, safety and traceability. This is also
aimed to enhance a fair value of a product along the chain of custody. The term bio-commerce
includes the activities of production/collection, transformation and commercialization of goods and
services derived from native biodiversity (genetic species, resources and ecosystems) that they
are developed of sustainable form, in accordance with ambient, social and economic criteria
(UNCTAD).There are many goods bearing green-labelling, such as, energy saving light bulbs,
hybrid cars to reduced CO2 emission, organic food, hand-netted reef fishes, managed wild-caught
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tuna and salmon. Another related term is ‘Geographical Indication’ (GI) - a name or sign used on
certain products or which corresponds to a specific geographical location or origin (e.g., a town,
region, or country). The use of a GI may act as a certification that the product possesses certain
qualities, or enjoys a certain reputation, due to its geographical origin such as Puntius denisonii
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(Day), from the Western Ghats, India.
Global Facts
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Ornamental fish is regarded today as a consumer based commodity and is the star product of
the pet markets. It is a multi-million dollar industry that supports thousands of rural people in the
developing countries. The global ornamental fish trade in retail level is worth more than US$ 8
billion with an average annual growth of 9%, while the entire industry including plants, accesso-
ries, aquarium, feed, medications etc. is estimated to be worth more than US$ 18-20 billion. The
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growing awareness of the need to respect the environment and, therefore, to know more about it,
has helped popularize aquarium keeping with an education component. With the increasing popu-
larity of household aquariums in many countries, ornamental fish play an important role in the
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international fish trade, providing employment opportunities to the rural population and considered
as an earner of foreign exchange for many developing countries. As per FAO statistics the global
estimates of exports of ornamental fish is around US$ 337 million in 2008 representing an in-
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crease of more than 12 per cent over the previous year. Exports since 2000 showed recovery in
buying patterns with most of the ornamental fish being sourced from the developing countries in
the tropical and sub-tropical regions of the world. About 60-65% of ornamental fish were supplied
from the developing countries.
There are more than 120 countries involved in ornamental fish trade. Altogether about 1,800
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species of fish are traded, of which over 1200 are of freshwater origin. More and more species are
being added to the list as a result of advancement in breeding, transport and aquarium technology.
The global ornamental fish industry, although relies largely on captive-bred fishes of freshwater
origin, significant numbers of fish and invertebrates from the wild are also included. The marine
fish species constitute more than 15% of the market by value. About 98% of marine fish are
collected from the wild while the rest are captive-bred. In general, the lower valued fish have
greater demand than the high valued species. It has been estimated that about 30 freshwater
species dominate the market such as guppies, platies, swordtails, mollies, neon tetra, angel fishes,
gold fish, zebra danio, discus and barbs. The guppies and the neon tetra alone represent more
than 25% of the global market in volume and fetches more than 14% in value. In the export
market, the share of Singapore, the top exporting country in trade of ornamentals was 20% fol-
lowed by Czech Republic (8%) Malaysia (7%), Israel (4%), Sri Lanka (3%) and China (1%). How-
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ever, little is known about the scale of the international trade in many species, and there are
concerns that trade in some species might not be sustainable, given factors such as their biology,
distribution, conservation status and ability to survive in captivity.
The Indian scenario
The Western Ghats and the North-Eastern Region of India are considered to be two of the 34
‘hotspot’ areas of the world for biodiversity conservation with a variety of vegetation types, climatic
zones and remarkable endemism. These regions are also endowed with a variety of brilliantly
coloured ornamental fishes. With an abundance of these living jewels, India has a distinctive edge
over other countries in trade potential. However, the Indian export of ornamental fish during 2009-
10 was only US$ 1.17 million (Rs.55 million) (source MPEDA ) Exporters have put forward a
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number of reasons for the backwardness of India in the world trade including breeding, freight
charges and lack of incentives. India has not made her presence felt in the international market for
various reasons viz., inadequate transport facilities /high air freight, non-availability of breeding
stock of exotic fishes, lack of professional training in nutrition, disease management, health care
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and breeding of exotic and indigenous fishes, lack of training in packing, handling and transporta-
tion, poor marketing strategies and restriction on the marketing of marine fishes and invertebrates.
There are no definitive surveys on the domestic market size of ornamental fish also. As per the
available data, the domestic ornamental fish market is worth around Rs.50 Crore and the demand
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is increasing at 20%. Economic surveys indicated that 16 million house holds (i.e. 8% of the 200
million house-holds) will require around 400 million fish per annum and can be optimistically as-
sumed that the domestic market in India offers a huge untapped market in the coming decade.
The MPEDA-UNCTAD funded survey conducted by Department of Industrial Fisheries, CUSAT in
the five states, viz. West Bengal, Tamil Nadu, Kerala, Karnataka and Maharashtra estimates an
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annual production of 100 million fish from 1,703 units occupying an estimated area of 160 hect-
ares. The productivity of ornamental fish sector in India is abysmally low when compared to the
production of 132 million fish from 156 hectare in Singapore.
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The abundance of both terrestrial and marine biodiversity in India has been both a blessing
and a curse for the country. Unbridled exploitation of resources has crossed the sustainable levels
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and has also led to the decline of a number of species of plants and animals. The range of species
caught and made available to the market often demands a continuous supply of “new fish species/
varieties” and the hunt from the wild has tremendously increased in the global trade. As more and
more ‘beauties’ from the rivers and coral reefs started attracting the world market, levels of exploi-
tation kept going higher and higher which had led to adoption of destructive fishing practices. With
other species of animals and plants currently in trade, new varieties have been produced by cap-
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tive breeding, but with aquatic ornamentals, this is not yet the case. Human interference has
disturbed the ecosystem of many water bodies also and only a few of them remain in pristine
condition. In several parts of the world, there is growing awareness among the beneficiaries and
user-agencies on the imperative need to conserve and manage various ecosystems. Official data
on commercial exploitation from the wild have always been lacking, and resource monitoring has
been poor. Therefore, the true extent and impacts of years of largely uncontrolled exploitation of
natural ornamental fish resources have only recently started to be recognized. In 2000, the Global
Marine Aquarium Database (GMAD) was established by UNEP-WCMC (United Nations Environ-
ment Programme- World Conservation Monitoring Centre), in collaboration with the Marine Aquarium
Council (MAC) and with members of various aquarium trade associations. Many industry mem-
bers (wholesale exporters and importers) provided data to enable monitoring of the trade in ma-
rine ornamentals, including information on the species in trade, volumes traded, and source and
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destination countries. While this initiative provided an important step for the monitoring of this
trade, it lacks an institutionalized, systematic reporting process and a regular source of funding to
sustain it. In addition to GMAD, a number of mechanisms exist in many countries which aim to
gather information concerning the trade in these organisms. These include customs and veteri-
nary border controls, and sustainability-certification schemes. However, to date, certification and
monitoring efforts have been focused on the marine component of the ornamental trade, with less
emphasis on the freshwater sector. Although much of the freshwater trade involves captive-bred
specimens, substantial volumes of wild-caught ornamental fish are also traded. Little is known
about the scale, nature and sustainability of the trade of wild-caught freshwater ornamental spe-
cies.
In order to address some of these issues, and to bring on board the various opinions of the
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different stakeholders, a workshop on sustainable harvest and green certification of wild caught
indigenous and cultured ornamental fishery resources for export was conducted by the Marine
Products Export Development Authority (MPEDA) in association with United Nations Conference
on Trade and Development (UNCTAD) and Project PIABA, Brazil, at Kochi, India during 14 – 18
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October, 2008.
The objectives of the workshop included
❖
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promoting environmentally and socio-economically sustainable ornamental fish trade
expanding & diversifying market through green certification
creating geographic indication and brand image for the Indian fishes.
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❖ Identifying significant freshwater ornamental fishes to be listed under GI from India.
❖ prepare guidelines for green certification standards for various stages in the supply chain.
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❖ Formulating guidelines for green certification of ornamental fish species.
Task Force
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The workshop recommended that a Task Force be constituted for formulating the guidelines
for Green Certification for Wild caught and cultured ornamental fishes in the domestic and export
trade with the following terms of reference:
a) Formulate a Green Certification system for wild caught and cultured ornamental fish
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b) Prepare a road map for implementing the system.
c) Submit the Green certification system developed for the approval of international organiza-
tions such as Food and Agriculture Organization of the United Nations (FAO), Ornamental
Fish International (OFI), Ornamental Aquatic Trade Association (OATA) and World Wildlife
Fund (WWF).
Accordingly, a National level Task Force was constituted by the MPEDA as per order no : 5/
2(B)/OFD/HO-2007 Vol 2-B dated 04.12.2008 under the Chairmanship of Dr E. G. Silas, Former
Vice-Chancellor, Kerala Agricultural University with the following members in the Task Force:
1. Dr. E.G. Silas (Chairman), 37- Ambady Retreat, Chilavannoor Road, Kochi, Kerala- 682020
([email protected])
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2. Dr. A. Gopalakrishnan, Principal Scientist, National Bureau of Fish Genetic Resources (NBFGR)
Cochin Unit, CMFRI Campus, Kochi, Kerala – 682018. ([email protected])
3. Dr. A. Ramachandran, Professor, School of Industrial Fisheries, Cochin University of Science

and Technology, Kochi, Kerala- 682016 ([email protected])
4. Dr. T.V. Anna Mercy, Professor, College of Fisheries, Kerala Agricultural University, Panangad,
Kerala -682506 ([email protected])
5. Mr. Kripan Sarkar, Ornamental Fish Breeder, Rainbow Fish Farm, New Jalpaiguri, West Ben-
gal ([email protected])
6. Mr. K.R.Pushpangadhan, Exporter, Travancore Aquapets, IV/342 A, Kollayil House, Kumbalam
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P.O., Kerala - 682506 ([email protected])
7. Dr. M.K. Ram Mohan, Deputy Director, MPEDA, Kochi - 682 036, Kerala
([email protected])
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7. Mr. Anilkumar. P, Technical Officer, INFOFISH, Kualalumpur, Malaysia – Convenor (till
14.09.2009) ([email protected])
8.
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Dr. Anikuttan.K.K. Assistant Director (OFD), MPEDA, Kochi - 682 036, Kerala – Convenor
from 15.09.2009 onwards. ([email protected])
The report prepared by the Task Force was sent to various national and international bodies
for comments and suggestions which were incorporated and finalized after a series of delibera-
tions to the present form.
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PROTOCOLS AND PROCEDURES

1. COLLECTION OF ORNAMENTAL FISHES AND INVERTEBRATES FROM THE NATU-
RAL WATER BODIES
1.1. Source of water – do not collect from water bodies which are contaminated by pollutants,
chemicals, fish poisons, explosives and locations where OIE listed pathogens occur.
1.2. Do not use fish poisons and explosives for collecting fish
1.3. Do not collect fish from sanctuaries, protected areas including sacred water bodies, fish
breeding grounds and during breeding seasons. Sanctuaries, Protected areas and desig-
nated areas should be delineated. Commercial exploitation from reserve forests and forest
areas should be strictly prohibited. Participatory support from the local communities will be
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essential for the implementation. Awareness programmes have to be developed for this
purpose
1.4. Identification of season for the best collection- State Fisheries Department has to notify the
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closed season, quota for fish catch and other regulatory measures to protect and manage
the resource.
1.5. The Central and State acts related to conservation measures to be strictly followed
1.6. Method of collection IN
1.6.1. Use encircling nets, small drag nets, bag nets, traps and dip nets of appropriate
mesh size only which will allow escape of undersized fishes. However, by-catch, if
any should be released back.
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1.6.2. Species specific eco-friendly gears only have to be used. Do not use cast nets, gill
nets and knotted nets.
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1.6.3. Natural aquatic flora should not be destroyed during collection.

1.6.4. Collection should be timed for early morning, evening or night
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1.6.5. The water from the collection site should be kept ready for transfer of fish with scoop
net.
1.6.6. Always keep the collection bags or containers with fish submerged and away from
direct sunlight to prevent rise of temperature.
1.6.7. Do not carry fish during collection
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1.7. Handling and transfer of fish

1.7.1. Prevent overcrowding of fish in the bags/containers to avoid stress and mortalities
1.7.2. Do not take the fish out of water while transferring to collection bags and avoid un-
necessary handling.
1.7.3. Use hand net/ scoop net made of soft cloth only, such as cotton/linen to avoid any
injury while transferring fish.
1.7.4. Once the collection is completed the fish should be immediately transferred to condi-
tioning facility.
1.7.5. Battery/generator powered aerators are advisable during handling and transportation
of the fish.
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2. PRIMARY HOLDING FACILITY (model lay out given in Annexure - 1)

2.1. It should be a fish collector’s society/cooperative society/ Self Help Group (SHG)/individual
registered with the authority concerned of the State and MPEDA.
2.2. First level certification can be availed only if the holder and holding facility complies with the
guidelines stated in 1.6 and 1.7
2.3. Select the site for holding facility where clean and good quality natural water is available.
Incase of bore well water, quality has to be tested for compatibility and continuous monitor-
ing is required.
2.4. If ground water is used it should be aerated for a minimum period of 24 hours
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2.5. Where ever possible rainwater harvesting can be done. Before using the rain water the ionic
content should be balanced and biosecurity ensured.
2.6. Freshly caught fish should be kept for a minimum of 48 hours during which no feed should
be given
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2.7. Minimum facility such as cement tanks /FRP/Glass and aeration facilities for holding the fish
2.8.
2.9.
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Packaging and transport- at stable/ reduced temperature (should be in the oxygen filled
bags during early morning/evening/night)
Transport of fish from Primary to Secondary facility should be in insulated containers.
2.10. The discharge water from the facility should be treated before release.
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3. SECONDARY/ MAIN HOLDING FACILITY (model lay out given in Annexure-2)
3.1. Water storage facility is needed. For source and quality of water, adhere to the conditions
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stipulated at points 2.3. 2.4 and 2.5. Water quality should be tested by an approved labora-
tory every six months.
3.2. Site should be away from the areas that are prone to flooding
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3.3. Use artificial tanks such as cement/ plastic/ fibre/ glass tanks only
3.4. Nets and other implements should be separate for each tank
3.5. Fish acclimatization area preferably in FRP/ plastic/ glass tank
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3.6. Packaging and transport as per BMP

3.7. Permissible antibiotics and anesthetics may only be used (Annexure - 3)
3.8. While receiving the fish at the holding facility dip treatment with suitable disinfectants/chemi-
cals may be given.
3.9. Drainage from the acclimatization facility should be separate and this water should be treated
and it should not be released into natural water bodies. Discharge of the water should be
carried out as per Pollution Control Board’s guidelines.
3.10. The quantity of fish stored should not exceed the permissible holding capacity and there
should be extra holding facility in reserve to meet exigencies.
3.11. Laboratory with minimum facility to assess the water quality (Nitrite, Ammonia, Oxygen,
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Carbon dioxide , pH, Temperature and Hardness) and to check fish health (external clinical
signs)
3.12. Dead fish should be disposed after post mortem following standard sanitary procedure.
4. EXPORTING FACILITY: (model lay out given in Annexure - 4)
4.1. Fish should be collected only from Green certified facility
4.2. Pre-shipment quarantine facility is essential for holding the fish for a minimum 7 to 45 days
depending up on the species and size
4.3. Pre-shipment quarantine should be done in smaller glass tanks with independent inlet and
outlets.
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4.4. Better Management Practices for packing to be followed.
4.5. Health certification from the authority concerned be obtained before shipment
4.6. Import requirements of the buyers with respect to health and other standards have to be
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fulfilled.
4.7. For source and quality of water, adhere to the conditions stipulated at points 2.3, 2.4 and
4.8.
5.
2.5.
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Adequate biosecurity measures must be in place to ensure pest and bird control, prevention
of contamination and escape of fishes into natural water bodies.
CULTURE FACILITY ( Model lay out given as Annexure - 5)
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5.1. General Requirements
5.1.1. Adopt Hazard Analysis Critical Control Point (HACCP) principles – for evolving
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the general process control strategies.

5.1.2. All buildings, ponds and tanks complete with labels and dimensions must be shown
in floor plans
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5.1.3. Production area shall be at least 70% of total farm area

5.1.4. Master farm should have completely drainable ponds.
5.1.5. Organic manure, especially animal wastes (eg. slaughter wastes) should be
avoided.
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5.1.6. In case of water shortage, water recirculation system can be used with adequate
filtration.
5.1.7. Pollution control board guidelines should be followed for treating water (http://
www.cpcb.nic.in/)
5.1.8. The drains should be covered.
5.2. Mandatory building/facilities to be constructed
5.2.1. Fish ponds and tanks (cement/ Fibre Reinforced Plastic (FRP)/ glass tanks)
5.2.2. Reservoir tanks and overhead storage tanks
5.2.3. Quarantine cum conditioning area
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5.2.4. Fish disease treatment area, lab and chemical store- (Fish Health Monitoring and
Management Section).
5.2.5. Fish feed preparation/storage area.
5.2.6. Fish holding area
5.2.7. Packing area
5.2.8. Water and air supply
5.2.9. Electricity / Generator /Fuel store
5.2.10. Sedimentation pond
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5.2.11. Effluent Water treatment plant
5.2.12. Office and Accommodation / Rest room
5.3. Site selection
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5.3.1. Designated wet lands such as mangrove areas / swamps and forest lands should
not be selected for setting up of the unit.
5.3.2.
5.3.3.
5.3.4.
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The site should be free of pollution, including seepage of pollutants.
The site should not be in flood plains/saline soils.
The site should be located in a place where key inputs such as sufficient good
quality water (including bore well and harvested rain water) and electricity are
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available.
5.3.5. The site should have good connectivity to markets and airports.
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5.3.6. Adequate precautions should be taken while extracting and discharging water to
maintain good water quality and environment while operating the unit at all times.
5.3.7. There should be adequate precautions to prevent the farmed fishes and alien
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species from escaping to the natural water bodies.

5.4. Source of Water
5.4.1. Municipal water meant for drinking should not be used.
5.4.2. River /pond/open well water needs to be filtered through filters such as bio-filter
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and sand filter. The sand filtered water may be ozonized or UV treated. No chemi-
cal should be used for treatment.
5.4.3. Bore well water, after agitation should be treated to remove iron, Hydrogen Sul-
phide, hardness etc. as the case may be. Care should be taken to recharge the
ground water with the treated discharge water.
5.4.4. Where ever possible rainwater harvesting may be resorted to as an alternate means
of water supply and to recharge the ground water.
5.4.5. PVC pipes alone should be used for the distribution of water.
5.4.6. Reservoir and overhead tanks of required size should be maintained to store and
distribute water
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5.5 Infrastructure Required - Ponds & Tanks (Ponds for brood stock maintenance and larval
rearing).
5.5.1. Fish ponds:
5.5.1.1. Arrange in a manner to facilitate management and harvesting
5.5.1.2. Separate each other by 1m all around
5.5.1.3. Provision must be there for draining completely to dry
5.5.1.4. Inlet, outlet, gradient/slope should be provided for each pond
5.5.1.5. Recommended to have a pond size of 20 sq.m.
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5.5.1.6. Anti predator netting and fencing should be used
5.5.1.7. Earthen ponds lined with Poly Urethane material provided with adequate bio-se-
curity measures may be used.
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5.5.1.8. Scoop nets and harvesting nets should by hygienically treated before and after
every use.
5.5.1.9.
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Nets and implements should be separate for each tank/ponds. The nets used for
the ponds should be disinfected before use as per the procedure given in Annex-
ure 6.
5.5.1.10. To avoid loss of fish, prevent contamination and predation screen and nets should
be used.
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5.5.2. Fish tanks
5.5.2.1. Should have a provision for inlet and outlet of water for each tank. The tanks
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should be constructed in modules for good management practice.

5.5.2.2. Aquaria and brood stock tanks should be in a proper building with adequate light-
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ing arrangements.
5.5.2.3. All tanks should be provided with Biological filter. The biological filter should be 5-
10% of the water volume. 10% of the biological filters should be maintained as
reserve to meet any contingencies with proper power back up.
5.5.2.4. All tanks should be provided with proper aeration. Two air stones (having dis-
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placement of one liter per minute) per thousand liters of water be provided for
aeration and filtration.
5.5.2.5. The cement tanks may preferably have plastic/epoxy/fiber coating.
5.5.2.6. Tanks shall be arranged back to back
5.5.2.7. Tank size should be as per species requirement.
5.5.2.8. A space of about 0.8m between each group of fish tank to facilitate worker’s move-
ments.
5.5.2.9. Should be connected to main drainage system which lead to sedimentation ponds
5.5.2.10. Anti predator netting should be used
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5.5.2.11. Aeration & biofiltration system should be separately provided for each tank
5.5.2.12. Separate hand nets should be provided for each tank.
5.5.3. Reservoir tanks and overhead tanks (For Holding water for distribution to fish ponds/tanks &
Receiving recycled water)
5.5.3.1. To be located next to sedimentation pond
5.5.3.2. Prior to discharge in reservoir ponds/tanks fish pond effluents must be discharged
in sedimentation pond
5.5.3.3. Reservoir tank capacity should suffice the supply of daily requirement of water.
5.5.3.4. A storage tank having capacity of minimum 10% of the total requirement of water
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be provided. The storage tank should be covered properly.
5.5.3.5. Recommended to have rain water harvesting & storage facility.
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5.5.4. Supply & drainage system
5.5.4.1. Direction of flow must be indicated on plans
5.5.4.2.
5.5.4.3.
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Connection between sedimentation pond and reservoir pond must be shown.
Drainage for the surface system (with the exception of quarantine area and fish
disease treatment area) shall be linked to reservoir pond/tank.
5.5.4.4. The discharge water should be treated properly before release.
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5.5.5. Quarantine cum conditioning area
5.5.5.1. Location should be close to the road entrance
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5.5.5.2. Access to and from the quarantine area should be restricted so as to avoid cross
contamination.
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5.5.5.3. Foot bath should be provided at the entry to the quarantine and holding area.
Foot bath should cover the entire length of the entrance and it should be of at least
30 cm depth and 75 cm width. This footbath must be changed regularly to main-
tain its efficacy.
5.5.5.4. Tanks should have adequate viewing facility to observe fishes for behaviour and
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signs of any distress or pathology

5.5.5.5. Drainage system should not lead into culture/ sedimentation ponds.
5.5.5.6. Separate water line connection should be provided to avoid any cross contami-
nation with the holding facility. Disinfected water must be used in the quarantine
area.
5.5.5.7. Separate the quarantine area completely by a brick wall.
5.5.5.8. Feed store, disease treatment area and hatchery/production areamust not be in
the same area as quarantine area.
5.5.5.9. Floor should be made up of durable and graded tiles which are easy to clean.
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5.5.5.10. Each tank should have separate siphoning or tank cleaning equipment and nets.
5.5.5.11. Personnel working in the quarantine area should not work in other bio secured
areas; eg. Holding area.
5.5.5.12. Freshwater fishes entering quarantine should be given a saltwater dip on the way
into the system and, if necessary, two more saltwater baths at 3- to 5-day inter-
vals.
5.5.5.13. Quarantine period should be 7 to 30 days depending on the species and the type
of diseases they are susceptible to
5.5.5.14. Temperature of the quarantine section to be maintained between 24 – 28 0C.
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5.5.5.15. Prophylactic treatments – Fishes should be examined for any clinical signs of
diseases / stress and appropriate prophylactic treatments such as formalin/ Po-
tassium Permanganate bath/ salt treatment,may be given. .
5.5.5.16. Before the disposal of dead fishes, post mortem examination should be conducted
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in a systematic sequence to identify the causative factor and records maintained.
5.5.5.17. Proper documentation of the details of the fish received, transferred to holding
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tanks, treated, dead and disposed should be maintained.
5.5.5.18. List of permitted antibiotics/chemicals – given in Annexure-3
5.5.6. Fish health monitoring and management section.
5.5.6.1. The fish health management section should be well isolated from the main unit,
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with an independent water supply and storage system.
5.5.6.2. The area should be composed of tanks for maintaining the sick fish (hospital tanks)
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with sufficient aeration.

5.5.6.3. A separate set of equipment (including nets) should be kept for each tank.
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5.5.6.4. The hospital tank need not have the conventional type of biological filtration.
5.5.6.5. The hospital tanks should be properly labelled and should be with a lid to avoid
aerosols.
5.5.6.6. There must be a separate tank maintained for disinfecting nets on regular and
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continuous basis following an approved method of disinfection (Annexure 6).

5.5.6.7. All dead fishes must be removed instantly from the tanks and placed in a plastic
bag that is clearly identified with the tank number. These fishes could be subjected
for either disease diagnosis or disposed off after post mortem (Annexure-6).
5.5.6.8. When used for disease diagnosis they could be maintained at 40C for a short span
of 3 to 4 hours for the detection and isolation of bacterial or fungal pathogens or at
-200C if the assay is for viruses.
5.5.6.9. The representatives of diseased samples should be sent to nearby authorised
diagnostic laboratory for further analysis; transportation should always be at low
temperature as stipulated in Annexure - 7.
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5.5.6.10. Disposal should be in 10% formalin kept in a closed carboy (Annexure-6).

5.5.6.11. Records should be maintained for all treatments, indicating the dosage, nature of
treatment (short bath, prolonged immersion, oral medication or injection). The
results of treatment should also be indicated.
5.5.6.12. The effluent water from the hospital tank should be properly treated before dis-
posal. (Annexure-6)
5.5.6.13. Fish Health Monitoring and Management Section should be equipped with a labo-
ratory for primary observation of the clinical signs of diseases, refrigerator with a
freezing cabinet, facility for water quality analysis and a store for prophylactics
and therapeutants.
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5.5.6.14. Laboratory for primary observation of clinical signs should have three sections
which are compartmentalized with passage having door with automatic door closer.
Part of the lab may be set apart for maintaining chemicals used for the preparation
of reagents as well as for chemotherapeutants and probiotics as listed in Annex-
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ure-3.
5.5.6.14.1. Diseased and moribund fish receiving and autopsy and biopsy section: The
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fishes after gross observation are subjected for autopsy and / or biopsy
(Annexure-8). The external and internal organs are observed using a mag-
nifying lens and samples are collected on slides for microscopic observa-
tion. This section should be equipped with the items listed in Annexure-9
5.5.6.14.2. Microscopic observation section: This should be equipped with a binocular
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microscope (with oil immersion objective) and beakers with disinfectant for
disposal of slides.
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5.5.6.14.3. Water quality analysis section should be equipped with the items listed in
Annexure-10.
5.5.7. Fish feed preparation / storage area (as per the design and lay out provided in Annexure -
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5).
5.5.7.1. Live feed must be disease free and be produced and maintained hygienically in
the farm itself.
5.5.7.2. Dry feed must be stored properly to maintain quality.
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5.5.7.3. Feed quality standards – Fish feed additives such as growth promoting substances,
fish colouring agents, antioxidants and veterinary drugs should contain only sub-
stances permitted by the authorities concerned. The records of feed ingredients
used may be maintained.
5.5.8. Fish holding area (To hold the stock meant for export; comes after quarantine)
5.5.8.1. Use artificial tanks such as cement/ plastic/ FRP/ glass tanks only
5.5.8.2. Nets and other implements should be separate for each tank
5.5.8.3. Drainage from the holding facility should be separate and this water should be
treated and it should not be released into natural water bodies. Discharge of the
water should be carried out as per Pollution Control Board’s guidelines.
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5.5.8.4. The quantity of fish stored should not exceed the permissible holding capacity and
there should be extra holding facility in reserve to meet exigencies.
5.5.8.5. Dead fish should be disposed following standard sanitaryprocedure.
5.5.8.6. Feeding schedules must be strictly adhered to as per the requirements of the
species.
5.5.8.7. Model layout of the holding area is provided in the Annexure -5
5.5.9. Packing area
5.5.9.1. Packing water –good quality filtered water with good aeration should be provided
5.5.9.2. Accessories- Styrofoam box, polythene bags, master cartons. Only new polythene
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bags to be used and packing materials to be stored hygienically.
5.5.9.3. Packing machine
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5.5.9.4. Oxygen
5.5.9.5. Temperature control – maintain between 20 - 23 0 C
perature control.
5.5.9.7. Use new plastic bags
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5.5.9.6. Packed fish meant for export to be stored in a separate storage room with tem-
5.5.9.8. Packing must be done one day prior to transportation, and repacked for dispatch.
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5.5.9.9. Never use formalin in packing water. In general, the ratio of waterand air in bag
is 1:2 (water:air)
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5.5.10. Water and air supply equipments

5.5.10.1. Blower & Compressor
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5.5.10.2. Motor & Pumps

5.5.10.3. It is advisable to use more number of small capacity blowers than a
single blower of big capacity. Adequate back up of these machineries to
be provided as standby.
5.5.11. Electricity / Generator /Fuel store
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5.5.11.1. A standby generator should be provided.

5.5.11.2. Non conventional energy sources (solar/ wind energy) may also be uti-
lized.
5.5.11.3. Fuel store must be closed at all times and prohibited to be near inflam-
mable goods
5.5.11.4. Designs and maintenance must comply with safety standards and regu-
lations stipulated by relevant authorities.
5.5.12. Sedimentation pond (for settling of farm water)
5.5.12.1. Located next to effluent treatment plant and minimum size is 10m2
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5.5.12.2. Have walled partitions to direct water in a zig-zag or up-down manner to

facilitate sedimentation of silt, detritus and de- sludging
5.5.12.3. Details must be reflected clearly on plans
5.5.12.4. Discharge must be channeled to fish ponds/reservoir ponds.
5.5.12.5. Excess discharge must be directed to effluent treatment ponds for final
discharge.
5.5.13. Effluent water treatment plant - as per standards prescribed by State Pollution
Control Board.
5.5.14. Office and Accommodation / Rest room
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5.5.14.1. It should contain sufficient space to accommodate the following activi-
ties:
5.5.14.1.1. Administrative activities
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5.5.14.1.2. Stores for chemicals, feed, equipments
5.5.14.1.3. Canteen/dining hall
5.5.14.1.4.
5.5.14.1.5.
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Rest room for staff
Records maintenance
5.5.14.1.6. Library , computer / data processing centre
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5.5.14.1.7. Guest rooms
6. ENVIRONMENTAL INTEGRITY
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6.1. Farming should be planned and practiced in an environmentally responsible manner in

accordance with appropriate state, national and international rules and regulations.
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6.2. Breeding and farming of ornamental fish can impact on the environment in various ways
such as biodiversity, habitats and ecosystems; genetic diversity including Genetically Modi-
fied Organisms (GMO); endangered species, exotic species, alien and migratory species;
natural fish stocks and species; and water, soil and air quality. Farmers should ensure that
these impacts are identified, managed or mitigated including the production and marketing
of GMOs.
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6.3. Routine monitoring of the farm environmental quality such as water quality parameters and
disease combined with good record keeping is a pre requisite for all the units.
6.4. Hatchery produced seed may be used for culture rather than collecting the seeds from wild.
Breeding farms must be able to demonstrate that the brood stock is obtained legally
6.5. Responsible use of feeds, feed additives, manure and fertilizer that improves net energy
conversion and economic viability should be practiced.
6.6. Chemicals, veterinary drugs and antibacterial products may be used in a judicious manner.
6.7. Energy may be used responsibly to reduce any negative environmental impacts.
6.8. Farm bred ornamental fishes should not be released to natural water bodies
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6.9. Alien species should not be released to the natural water bodies.
7. BEST MANAGEMENT PRACTICES (BMP)
7.1. Live food has to be cultured within the premises to avoid cross contamination and infection.
7.2. Good quality, permissible ingredients only should be used for the prepared food.
7.3. Indiscriminate use of chemicals for modifying the natural colour of the fishes should be
avoided.
7.4. Dead and diseased fishes should be disposed in a sanitary way.
7.5. Feed should be given in feeding trays and not thrown directly into the water.
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7.6. The fish should be handled with minimum stress. The fish should not be exposed to air (not
be taken out of water) while transferring to another tank. Nets made of Soft clothing and of
appropriate size should be used. Separate nets should be used for each tank. While han-
dling, small sized fish a plastic cup like container should be used for scooping the fish.
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7.7. Packaging and transport fish as per the BMP.
7.8. Permissible antibiotics and anaesthetics/tranquilizers only be used (Annexure-3)
7.9. IN
Overcrowding of the fish should be avoided.
7.10. A laboratory to assess the water quality and fish health should be maintained as described
under section 5.4.4.
7.11. The green certified farms should procure fish only from green certified units.
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8. GREEN SEAL LABELLING : Green Seal labelling procedure has to be adopted to indicate
that the origin of the fish is from a certified operation and chain of custody to buyer and
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consumer. Hence Green certification is proposed as an independent certification and verifi-

cation programme for sustainable harvest, production and marketing of ornamental fresh-
water fish from India. The Green certification provides a guarantee that the certificate holder
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has procedures in place which ensures fishes supplied as green certified are authentic and
any associated claims are truthful and correct.
8.1. The two aspects of chain of custody (CoC) are formal interfaces between CoC operations
and defined procedures for fish tracking within each chain of custody operation.
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8.2. Green certification is required for any operation buying certified fishes that are used in the
CoC and sold to a client that wants to promote those fishes as certified or make a certifica-
tion claim about their origin.
8.3. Green seal certification requires that the ornamental fish in the CoC passes through the
established processes and standards of fish catching,handling,culturing , conditioning, pack-
ing and marketing. The certification also requires that the capture, culture and marketing
operations be improved to reduce mortality of fish, disturbances of ecosystem and sustain-
ing the resources in its natural habitat. The Green Seal is also concerned with environmen-
tally responsible, socially acceptable and economically viable procedure of production and
marketing of ornamentals.
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Green Certification Logo

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9. DOCUMENTATION & MAINTENANCE OF DATA –

It is mandatory to have proper documentation of all events and activities as well as mainte-
nance of the data for retrieval and verification for a unit to be accredited as a Green Certified unit.
Annexures – 11, and 11A to 11E.
10. AGENCY FOR ACCREDITATION

MPEDA will be the Nodal Accreditation Agency for implementation of Green Certification
System for freshwater ornamental fish in the country.
11. PROGRAMME IMPLEMENTATION

11.1. Short Term Plans
11.1.1. Verification Committee
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It is proposed that Verification Committees (VC) shall be constituted at regional levels by
MPEDA for verification of the chain of custody for compliance to the prescribed guidelines and to
recommend certification by MPEDA. The Committee shall comprise of a convenor (from MPEDA)
and three expert members, from the following orgnisations:
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(i) Central / State Universities
(ii) ICAR Fisheries Research Institute
(iii) State Fisheries Department
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When a unit applies for Green Certification, verification committee will undertake two visits to
the chain of custody, first for verification of required infrastructure and the second for verification of
system implementation. The time period between two visits shall not be more than 6 months.
Based on the 1st visit, a provisional certification for Green Certiciation will be granted to the unit,
and final certification will be granted subject to recommendation by the VC on compliance to the
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system implementation
Certification will be valid for a period of 1 year and shall be renewed.
11.1.2. Awareness programmes
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Awareness/Training programmes with time lines to be arranged by MPEDA

to the verification committee members and all the stakeholders.
11.1.3. Identification of units to introduce Green Certification on a voluntary basis
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(Demo project)
MPEDA may identify some leading ornamental fish collection, breeding and
exporting units to introduce Green Certification on a voluntary basis and to
collect the required information to streamline the system.
11.1.4. To develop a monitoring and surveillance plan.
11.1.5. Periodical review of the guidelines and its implementation.
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11.2. Long Term Plans

Enhance the research programmes on ecology and biology of native ornamental fish
species, their abundance and availability, mapping of geographical distribution, stock
specific rehabilitation, conservation and management of the resources.
12. STANDARDS FOR ASSESSMENT

12.1. Ecosystem and fishery management
Ecosystem and fishery management standards have to be evolved based on regular
feedback and stake holder consultations for sustainable utilization of the freshwater
ornamental fishes.
The Marine Products Export Development Authority (MPEDA) will be the nodal agency
to coordinate and constitute appropriate committees to address the ecosystem and
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sustainable resource management aspects in consultation with respective State ad-

ministration. The recommendations by the committees may be implemented by the
State Governments concerned. The concerned authorities have to study the status of
the wild fish stocks on a continuous basis and maintain a database. Studies on fixing
up Minimum Legal Sizes (MLS) and exploitable stock in each region also shall be orga-
nized. The MPEDA shall be the repository as well as the disseminating agency of the
data.
12.2. Chain of custody management
The authority concerned identified by the MPEDA has to verify that the chain of custody
activities (including collection, holding, culture, transport and marketing) conforms to
the ecosystem integrity of the collection area, sustainable use of the fishery resource
and good health of the harvested fish. The detailed guidelines addressing these issues
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are contained in this document.
12.3. Score cards for assessment
Performance evaluation in the format of score cards have been evolved for the various
facets of the value chain of Green Certification. The details of these are given in
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Annexures -12A to 12E. The unit which scores more than 75% when assessed shall
qualify for Green Certification. For a fish to be designated as Green certified, it should
pass through the entire green certified chain of custody.
13. IMPORT OF EXOTIC FISHES

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Guidelines for the Import of Ornamental Fishes into India” issued by Ministry of Agriculture
based on the recommendations of National Committee on Introduction of Exotic Aquatic Species
into India must be followed (Annexure- 13).
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14. GEOGRAPHICAL INDICATION OF SPECIES
Geographical Indications (GI): The article 22 of the Trade Related Aspect of Intellectual
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Property Rights (TRIPS) agreement of India defines, Geographical Indications (GI) as “indications
which identify goods as originating in the territory of a member, or region or locality in that territory,
where a given quality, reputation or other characteristic of the good essentially attributable to its
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geographical origin”. It is assigned to give consumers confidence that product they buy come from
specific place with repute and it has certain characteristics that are due to that place of production.
They are important for product of plant varieties or animal breeds that have gained favorable
reputation because of their geographical association. As obligations under the WTO regime and
other international agreements, India has taken suitable steps and enacted several acts, including
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the Geographical Indications of Good (Registration and protection) Act, 1999 that has come into
force since 2001 in harmony with the TRIPS provisions. The Act seeks to provide for the registra-
tion and protection of goods related with geographical indications in India. “Indication” includes
any name, geographical or figurative representation or any combination of them conveying or
suggesting the geographical origin of goods to which it applies. “Goods” means any agricultural,
natural or manufactured goods or any goods of handicraft or of industry and includes food stuff”
originating or manufactured in a region or locality of a territory, where a given quality, reputation or
other characteristics of such goods can essentially be attributed to (Section 2; The Fourth Sched-
ule). An applicant under section 17 can be a producer, any association of persons, users, organi-
zation or authority established under the law (Section2) and must represent the interest of the
producers or authorized user in respect of a reorganized geographical indication (Chapter III). The
producer has been defined as the person(s) who produces the goods, or if such goods are natural
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goods, exploits the goods. The authorized users can exercise the exclusive right to use the geo-
graphical indication in promotion of the goods.
India is endowed with rich agro-climatic diversity associated with equally rich cultural heritage
belonging to one of the oldest civilizations of the world. Every region in India has its claim to fame
with some or the other agricultural products or practices with unique features that can be a source
of trade advantage. Specific Geographical indication should be able to help protect a product
produced by a number of different producers (concept similar to community, tribes, and ethnic
group), based on traditional practices or processes, which are passed on from one generation to
the other. It also relates to products, which normally have a relationship with the land, local re-
sources and the environment. Traditional knowledge is linked to a specific process related with a
place or zone, which confers the products with specific characteristics or reputation primarily be-
cause of its geographical origin or association Agro-biodiversity from specific areas such as high
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mountains of Himalayas, the Gangetic plains, arid regions of Rajasthan, tropical wet Northeast
region and the Western Ghats, have a number of bio-resources/products associated with specific
ecologies. There are many examples of traded agricultural products related with taste and quality
attributes, associated to specific geographical region. Such products get trade advantage, only
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because they are cited along with their place of production. For example, ‘Banarasi Pan’, ‘Melihabadi
Mango‘, ‘Allahabadi Amrood’, ‘Nagpur Santra’, ‘Goan Feni’, ‘Kannauj Rose perfume’, etc. in case
of plants, and Murra Buffalo from Haryana and Western Uttar Pradesh with higher miltching Bellary
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sheep from Andhra Pradesh and Tamil Nadu with quality wool and meat, Chokla sheep from
Rajasthan with white wool, Marwari goat from Rajasthan with quality meat, Aseel chicken with
dark flesh from Madhya Pradesh and Chattisgarh, Kathiawari horse from Madhya Pradesh, Kutchi
camel from Gujarat etc. among animals.
There is an urgent need to build proper strategy and infrastructure to claim protection for as
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many biodiversity derived freshwater ornamental fishes as possible. Registering them under GI
Act can help build a better platform for development of products of importance from trade point of
view and check bio-piracy of our nation’s genetic wealth. The major problem of enforcing GI pro-
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tection stems from the fact the GIs are, in a way, a collective mark. They are collectively held by
many small producers that do not have the resources to engage in expensive litigation and regis-
ter their names in all member countries of the WTO. Therefore, there is a need –
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1. for developing awareness about GI Act and the provision available in it for protection of prod-
uct and process associated with specific regions,
2. Popularization and motivation of local producers or users to join hands to form a strong asso-
ciation and maintain specific quality related to the reputation of the product to harness trade
and economical benefits.
3. In case of crop varieties and animal/fish breeds it may require some special efforts for appli-
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cation of principles of maintenance breeding by which the specific quality of the product is
maintained. This may need participatory approach involving relevant scientists and stake-
holders.
4. Formulation of association or cooperatives or motivating civil societies and non-governmental
organizations seeking registration of products and promoting market research and marketing
so that they become beneficial meeting the aspiration of local communities and are promoted.
Organizations such as MPEDA and ICAR may take lead for protection of fish genetic re-
sources geographically associated with some region for their specific characteristics, using GI Act.
This would require concerted efforts to undertake the following activities.
1. Identification of such genetic resources and their categorization as per fourth schedule of
classification of goods for the GI Act 1999
2. Geographical map of the territory of the country or region or locality in the country in which the
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goods are produced or originate or manufactured, describing physical characteristics of the

associated region in terms of-
a. Habitat characteristics
b. Temperature and relative humidity regimes at the harvest/production time
c. Annual seasonal precipitation
d. Agro-climate or production system
3. Characterization and validation of characteristics through appropriate indicators or param-
eters for establishment of geographical association:
a. Identification of standard benchmark
b. Identification of mechanism and components of unique characteristics
c. Range and mean of these components on a quantitative scale
d. Identification of contribution of these components to associated unique characteristics
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e. Level of stability/variability
4. How the geographical indication serves in respect of specific quality, which can be exclusively
or essentially attributed to geographical environment with its inherent natural or human fac-
tors.
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5. Commercial value/potential
6. Listing of producers/artisan (tribe or ethnic group) related with product
On confirmation the relevant products can be registered with requisite supportive/validated
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data and/or documented information by producer(s), users or association their off following
the procedure. This may need formation of farmers’ consortiums and licensing for getting the
trade benefits.
Proposed native freshwater ornamental fish species of India listed for GI:
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Sl. No. Scientific name Trade name Geographical area
1. Puntius denisonii (Day)* Redline torpedo Southern Western Ghats
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between 80 and 16 0 N. Lat.

2. Puntius chalakkudiensis Menon, Redline torpedo Western Ghats-Kerala (Thrissur,
Rema Devi & Thobias* Ernakulam & Idukki Districts)
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3. Puntius exclamatio Pethiyagoda 3 spot barb Western Ghats-Kerala (Quilon

& Kottelat* District)
4. Puntius filamentosus Indian tiger barb Western Ghats
(Valenciennes)*
5. Puntius rohani Rema Devi et al. Kanya barb Kanyakumari, Western Ghats
6. Puntius manipurensis Menon, Manipur barb North East India
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Rema Devi & Vishwanath*

7. Puntius fasciatus (Jerdon)* Melon barb Western Ghats
8. Puntius sahyadriensis Silas* Khavli barb Western Ghats- Maharashtra
region
9. Puntius pookodensis Anna Mercy Pookode barb Western Ghats – Wayanad District
& Eapen Jacob*
10. Puntius ornatus Vishwanath Ornate barb North East India
& Juliana
11. Puntius bizonatus Vishwanath North East India, Manipur
& Laisram
12. Puntius narayani (Hora)* Narayani barb Western Ghats - Karnataka,
Maharashtra, Goa.
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13. Puntius jerdoni (Day)* Jerdon barb Western Ghats - Kerala, Karnataka
14. Puntius ophicephalus (Raj)* Elongate barb Western Ghats – Kerala
15. Puntius tambraparniei Silas* Silas Barb Western Ghats - Tamil Nadu
(Tirunelveli Dt.)
16. Puntius arulius (Jerdon)* Aruli barb Western Ghats Kerala, South
Karnataka.
17. Puntius melanostigma (Day)* Wayanaad barb Western Ghats - Wayanad
18. Puntius sarana subnasutus Olive barb Western Ghats- Karnataka, Kerala,
(Valenciennes)* Tamil Nadu
19. Gonoproktopterus curmuca Curmuca barb Western Ghats- Kerala, Karnataka
(Hamilton)*
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20. Gonoproktopterus thomassi Nilgiri shark Western Ghats- Kerala, Karnataka
(Day)*
21. Labeo nigriscens Day* Black labeo Western Ghats- Kerala, Karnataka
22. Labeo potail (Sykes) * Deccan labeo Western Ghats- Maharashtra,
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Karnataka
23. Horadandia attukorali brittani Glow light carp let Kerala
(Menon)
24.
25.
Tor malabaricus (Jerdon) *
Rasbora ornatus Vishwanath &

Laisram
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Malabar mahseer Western Ghats- Kerala,
Tamil Nadu
North East India, Manipur
26. Garra stenorhynchus (Jerdon)* Nilgiri gara Western Ghats- Kerala, Karnataka
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27. Garra hughi Silas* Cardamom gara Western Ghats- Kerala
28. Garra surendranathanii Shaji, Elongate garra Western Ghats- Kerala
Arun & Easa*
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29. Devario malabaricus (Jerdon) * Malabar danio/ Blue danioWestern Ghats- Kerala, Karnataka,
Tamil Nadu
30. Devario neilgherriensis (Day) * Nilgiri danio Western Ghats- Tamil Nadu
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31. Betadevario ramachandrani Broad-band Danio Western Ghats – Shimoga,

Pramod et al.* Karnataka
32. Brachydanio jaintianensis Red-line Danio North East India - Meghalaya
Nibedita Sen*
33. Barilius bakeri Day* Malabar baril Western Ghats-Kerala,
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Tamil Nadu, Karnataka

34. Barilius barna (Hamilton) North East India, Yamuna River
35. Barilius canarensis (Jerdon) * Jerdon’s baril Western Ghats- Kerala, Karnataka
36. Barilius dogarsinghi Hora Manipur Baril North East India - Manipur
37. Salmostoma horai (Silas)* Hora razor-belly minnow Western Ghats-Karnataka
38. Laubuca fasciata (Silas)* Malabar hatchet chela Western Ghats-Kerala, Tamil Nadu
39. Laubuca dadyburjori (Menon) * Dadio Western Ghats-Kerala, Karnataka,
Maharashtra
40. Osteochilichthys nashii (Day) * Nash’s barb; Malabar Western Ghats-Kerala, Karnataka
Osteobrama
41. Osteobrama bakeri (Day) * Malabar Osteobrama; Western Ghats-Kerala
Gurda Bakerova
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42. Lepidopygopsis typus Raj* Peninsular hill trout; Western Ghats- Kerala
Ostrozub travancorský,
Brahmana kenda,
43. Homaloptera montana Herre Anamalai loach Western Ghats- Tamil Nadu
44. Travancoria elongata Lizard loach Western Ghats-Kerala
Pethiyagoda & Kottelat*
45. Travancoria jonesi Hora* Travancore loach; Balitora Western Ghats-Kerala
Jonesova (Czech)
46. Mesonemachilus triangularis (Day)* Zodiac loach Western Ghats, South
47. Mesonemachilus guentheri Guenther’s loach; Western Ghats- South
(Day) * M?enka Güntherova
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(Czech)
48. Longischistura striata (Day) * Striped loach Western Ghats- South
49. Schistura nilgiriensis (Menon) * Crimson loach Western Ghats-Kerala, Karnataka
50. Schistura denisoni denisoni Denison’s loach; Western Ghats-Kerala, Karnataka
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(Day) * Nemacheilus, loach
51. Schistura semiarmatus (Day) * Armatus loach Western Ghats-Kerala,
Tamil Nadu; Karnataka
52.
53.
54.
Schistura prashadi (Hora)
Physoschistura elongata
Sen & Nalbant
Aborichthys garoensis Hora
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Elongate loach
North East India, Manipur
North East India, Shillong
Aborie garonská (Czech) North East India, Meghalaya

55. Syncrossus berdmorei (Blyth) Firetail Botia; Blyth’s North East India, Maniour
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Loach; Tiger Botia;
Berdmore’s Loach
56. Acanthocobitis moreh (Sykes) * Moreh loach; Western Ghats-Kerala, Tamil Nadu
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Nemacheilus loach;
Kannada name :
Murangi, malli; hunase
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57. Nemacheilus anguilla Annandale Eel loach; Western Ghats-Maharashtra

Black lined loach
58. Nemacheilus monilis Hora Monliform loach Western Ghats-Kerala, Tamil Nadu
59. Oreonectes evezardi (Day) Evezardi loach Western Ghats-Kerala, Karnataka
60. Indoreonectes keralensis Kerala loach Western Ghats-Kerala
(Rita & Nalbant)
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61. Lepidocephalichthys Ngakrijou macha; North East India.

manipurensis Arunkumar Nganap arangbi
62. Botia almorhae Grey Almorhae loach; Himalaya, India
Yoyo loach
63. Botia dario Hamilton Bengal loach; North East India.
Necktie loach
64. Botia rostrata Guenther Gangetic loach; India
Emperor loach;
Sekavka gan•ská (Czech)
65. Botia striata Rao* Tiger loach; Zebra loach Western Ghats - Maharashtra
66. Psilorhynchus sucatio River stone carp North East India, Assam,
(Hamilton) Darjeeling
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67. Psilorhynchus balitora Balitora, Ngop-nogi North East India, Assam,

(Hamilton) Darjeeling
68. Chaca chaca (Hamilton) * Frogmouth catfish; India
Square head catfish;
Indian chaca
69. Horabagrus brachysoma Yellow catfish Western Ghats-Kerala, Karnataka
(Guenther) *
70. Horabagrus nigricollaris White collared imperial catfish Western Ghats-Kerala
Pethiyagoda & Kottelat*
71. Glyptothorax anamalaiensis Silas* Striped sucker catfish Western Ghats-Kerala
72. Glyptothorax madraspatanam Brown sucker catfish Western Ghats-Kerala
(Day)*
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73. Horaglanis krishnai Menon Indian Blind catfish Kerala
74. Horaglanis alikunhii Blind catfish Kerala
Subhash Babu & Nayar*
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75. Akysis manipurensis North East India, Manipur
(Arunkumar)
76. Akysis prashadi Hora Indawgyi stream catfish; North East India, Manipur
77.
78.
Erethistes hara (Hamilton) *
Erethistes serratus
Vishwanath & Kosygin
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Seeprajokimonni
Butterfly catfish; Kosi haraIndia
Butterfly catfish North East India, Manipur
79. Conta pectinata Ng Conta catfish North East India, Manipur, Assam
& Meghalaya
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80. Microphis cuncalus (Hamilton) * Crocodile-tooth pipefish India
81. Microphis deocata (Hamilton) Deocata pipefish North Bengal, Assam
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82. Tetraodon travancoricus Red green dwarf puffer; Western Ghats-Kerala

Hora & Nair* Malabar Pufferfish
83. Carinotetraodon imitator Golden puffer; Western Ghats-Kerala
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Britz & Kottelat* Matkijapallokala; Pothal

84. Tetraodon cutcutia Hamilton* Figure eight puffer; India
Ostindisk Kuglefisk;
Intianpallokala;
Ocellated pufferfish
85. Badis badis (Hamilton)* Indisk bladfisk India
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86. Badis assamensis Ahl Assam badis North East India, Manipur, Assam
87. Badis tuivaiei North East India, Manipur
Vishwanath & Shanta
88. Polycanthus lalius (Hamilton) Dwarf gouramy North East India
89. Polycanthus fasciatus
(Bloch & Schneider) India
90. Polycanthus sota (Hamilton) North East India
91. Dario dario (Hamilton) Scarlet badis; India
Scharlakansbadis
92. Channa bleheri Vierke* Rainbow snakehead North East India-Endemic to the
Brahmaputra River basin, Assam,
India
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93. Channa barca (Hamilton) * Barca snakehead North East India

94. Channa aurantimaculata Orange spotted North East India Endemic to the
Musikasinthorn* snake head Brahmaputra River basin, Assam,
India
95. Channa diplogramme (Day) * Malabar snakehead; Western Ghats-Kerala
Indian giant snakehead
96. Etroplus maculatus (Bloch) * Orange chromide; Kerala
Gul cichlid
97. Etroplus canarensis Day* Canara pearl spot; South Karnataka
Elimeenu
98. Macrognathus guentheri (Day) * Malabar Spiny eel Kerala
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99. Pristolepis marginata Jerdon* Malabar catopra/Sun fish; Western Ghats-Kerala
Indian leaffish;
Malabar-bladfish
100. Pristolepis fasciata (Bleeker) * Sun fish. Western Ghats-Kerala
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101. Nandus andrewi Ng & Jaafar* North Bengal, Assam
102. Horaichthys setnai Kulkani Anu; Thready Top- West Coast of India (from Kutch to
Minnow; Malabar Ricefish Trivandrum, brackish)
103. Pseudosphromenus dayi
(Kohler)* IN
Spike-tailed paradise fish
* Photographs of fishes appended in Annexure - 16

Western Ghats - Kerala
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For ornamental fish GI here is defined here as the place of origin / first description of the
concerned species and its quality, reputation and other characteristics. GI registration of the above
species will expand the market for indigenous ornamental fish.
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14. CONCLUSION
Green Certification for the sustainable development is an emerging concept in the case of
fresh water ornamental fish. The International workshop organized by the Marine Products Export
Development Authority (MPEDA) in association with United Nations Conference on Trade and
Development (UNCTAD) and Project PIABA, Brazil, at Kochi, India from14 – 18 October, 2008 is
the first of its kind in the world. Consequently the National level Task Force constituted for prepar-
ing the Guidelines for Green Certification of ornamental fishes has come out with the Guidelines
and Geographical Indication of selected species after a series of brainstorming sessions and de-
liberations. The findings of the Task Force can be briefly summarized as follows:
❖ The ornamental fish sector is being operated in an unorganized manner at present with mini-
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mum scientific inputs and national policy involvements. This could be improved once the
green certification system is introduced for an effective value chain system.
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The increasing stringent export standards of the buyers call for a systematic and efficient
mechanism for monitoring and surveillance of the chain of custody in order to issue the nec-
essary certificates by the authorities concerned. The Green certification and Green Seal La-
❖
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belling would assure an eco-friendly healthy fish to the buyer.
The importance of maintenance of log books and records at all stages of the value chain is
emphasized and the necessary proforma have been appended to the report.
❖
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The floor plans for different facilities to maintain high standards in the handling and care of the
fish are appended.
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❖ These guidelines may be implemented to encourage voluntary participation of all stake hold-
ers.
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❖ Standards for assessment and necessary score cards for evaluation for the award of Green
Certification have been developed.
❖ A list of endemic freshwater ornamental fishes have been identified, as these have potential
for registration under GI Act, 1999 (Govt. of India). Trader consortiums/associations can uti-
lize this opportunity for exploring better market avenues and value addition.
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The MPEDA as the nodal agency may take appropriate action to implement Green Certifica-
tion and Green Seal Labelling for Freshwater Ornamental Fish of the country.
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ANNEXURE 3
List of permissible Medicines / Antibiotics/ Anaesthetics
for treating diseases
Antibacterial agents Worm removers
Oxytetracycline Fenbendazole
Oxolinic acid Mebendazole
Nifurpirinol Levamisole
Kanamycin
Ciprofloxacin
Antifungal agents Crustacean eliminators
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Acriflavine Nuvan (dichlorvos)
Formalin Trichlorfon
Malachite green (zinc free) Diflurobenzuron
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Methylene blue
Anti-protozoan Anaesthetics*
Leteux – Meyer mix MS222 (Tricaine methanesulfonate - Tricaine-S /Finquel
Copper sulphate
Metronidazole IN
Clove oil
Aqui-S (50% Isoeugenol and
50% Polysorbate 80)
CO2
Metomidate
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Benzocaine
* The buyers generally prefer not to use anaesthetics during transport. The appropriate dosage of anaesthetics may be
standardized for ornamental fishes.
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Therapeutics. 20: 333-349.
Piper, R. G., I. B. McElwain, L. E. Orme, J. P. McCraren, L. G. Fowler and J. Leonard. 1982. Fish
Hatchery Management. U.S. Fish and Wildlife Service, Washington, D.C.
Ross, L. G. and B. Ross. 1999. Anaesthetic and Sedative Techniques for Aquatic Animals. Blackwell
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Science, Inc., Malden, MA.159 pp.

Treves – Brown, K.M(2000). Applied fish Pharmacology. Kluwer Academic Publishers, London.
309pp.
Pramod P.K., Ramachandran A., Sajeevan T.P., Thampy S. & Pai S.S. (2009). Comparative effi-
cacy of MS-222 and benzocaine as anesthetics under simulated transport conditions of a tropi-
cal ornamental fish Puntius filamentosus (Valenciennes), Aquaculture Research, 41 (2), 309-
414.
Pramod, P.K., Sajeevan , T.P., Ramachandran, A., Sunesh Thampy S. & Somnath Pai (2010) Ef-
fects of two anesthetics on the water quality parameters during simulated transport of a tropical
ornamental fish Puntius filamentosus(Valenciennes). North American Journal of Aquaculture,
72, pp. 290-297
Sindhu M.C., 2009. Clove oil as an anaesthetic for transportation of Blue hill trout , Barilius
bakeri(Day) M.Phil. Thesis, Cochin University of Science and Technology, Kochi, India, 78 p.
27
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IN
A
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28
ANNEXURE 6
Disinfection & Disposal Methods
Disinfectants are compounds that reduce pathogenic organisms on biological surfaces by

rather non-specific actions. Ideal characteristics of a disinfectant in clued a broad spectrum, fast
action and low toxicity. For these reasons, as well as the lack of regulatory control, disinfectant has
gained wide-spread use in aquaculture facilities. Types of disinfecting agents that might be used
during an outbreak of disease in aquatic animals include the following: i) oxidising agents; ii) pH
modifiers (alkalis and acids); iii) aldehydes; iv) biguanides; v) quaternary ammonium compounds
(QACs); vi) ultraviolet (UV) irradiation; vii) heat; viii) drying; and ix) high temperatures.
Disinfectant applications for equipments and premises with recommended doses (as
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per Australian Aquatic Veterinary Emergency Plan, 2008).
Disinfecting Application Pathogens Recommended Comments
agent dose
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Hypochlorite Treatment of All pathogens Minimum 30 mg/L Use as a general disinfecting
solutions clean, hard available chlorine solution
(calcium surfaces
hypochlorite
or
sodium
hypochlorite)
Treatment of
water (assuming
low organic
loading)
All pathogens IN Minimum 30 mg/L
available chlorine
Maintain a minimum
of 5 mg/L of residual
chlorine.
Hold for a minimum of 24 hours
to inactivate. Test chlorine level
before discharge or neutralise
with thiosulphate. Less active in
the presence of high levels of
organic matter; Re-dose if
A
necessary
Treatment of net All pathogens Initial dose of 1000 Thoroughly mix to ensure even
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pens mg/L available distribution Immerse for a

Chlorine; Maintain a minimum of 6 hours.
minimum of 5 mg/L
of residual chlorine
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Dip treatment of All pathogens Solution of >200 mg/ Allow time to completely saturate
absorbent L available chlorine plus a further 2 minutes
material such as (minimum); Rinse items in fresh
dip nets, clothing, water or neutralise with
ropes or thiosulphate.
absorbent
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surfaces.
Treatment of All pathogens Spray with a solution Leave solution for 2 hours, then
tanks, floors and >1500 mg/L available rinse to free any remaining soils;
walls in culture chlorine Tanks should be filled with
facilities freshwater and dosed with 200
mg/L available chlorine; Leave
for 24 hours in the case of
whirling disease.
Chloramine-T Treatment of Bacteria, viruses, 20 mg/L of chloram- Hold for a minimum of 24 hours;
water fungi ine- T (or as per Test chlorine level before
manufacturer’s discharge or neutralise with
instructions) thiosulphate; Concentrations and
doses vary between products.
29
Treatment of Bacteria, viruses, 20 g/L of chloramine- Leave to dry on suitable

previously fungi T (or as per surfaces, or for a minimum of 30
cleaned hard manufacturer’s minutes before rinsing ;Concen-
surfaces instructions) trations and doses vary between
products.
Footbaths Bacteria, viruses, 50 g/L of chloramine- Brush boots clean before

fungi T (or as per immersion Leave to dry on boots;
manufacturer’s Concentrations and doses vary
instructions). between products.
Treatment of All pathogens 1% solution for Concentrations and doses vary

hard surfaces >60 minutes (or as between products.
per manufacturer’s
IA
instructions).
Peracetic acid Treatment of All pathogens 2% solution for >60 Concentrations and doses vary
porous surfaces minutes (or as per between products
manufacturer’s
D
instructions).
Treatment of All pathogens 40 L concentrate May cause excessive foaming

waste slurries solution per 1000 L and tank overflow in presence of
Monosulfate
compounds
(high organic
matter).
Treatment of
hard surfaces
All pathogens
IN Contact time >1 hour
10 g/L (or as per

manufacturer’s
high levels of protein.
Application rate of 400 mL/m2 for

>10 minutes; Doses and
instructions) concentrations vary between
A
products.
Treatment of All pathogens 20 g/L (or as per Application rate of 400 mL/m2 for
porous surfaces manufacturer’s >10 minutes; Doses and
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instructions) Concentrations vary between

products
Footbaths All pathogens 50 g/L (or as per Remove all organic matter on
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manufacturer’s footwear before immersion;

instructions) Immersion time >1 minute
Replace solution daily in areas of
heavy use; every 4 days in areas
of light use; doses and concen-
trations vary between products.
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Chlorine Treatment of All pathogens As per Can produce volatile fumes when
dioxide hard and porous manufacturer’s first activated
surfaces; instructions
Treatment of
water
Iodophores Treatment of Bacteria, fungi, >200 mg/L available Apply to surface 1–Iodophors 2
hard surfaces viruses iodine minutes.
Spray disinfec- Bacteria, fungi, 100 ppm available Apply to previously cleaned and
tion of equipment viruses iodine dried equipment.
Footbaths Bacteria, fungi, >200 mg/L available Clean boots before disinfection;
viruses iodine Replace daily in high-use areas,
or when solution has lost colour.
30
Use as a hand or Bacteria, fungi, > 200 mg/L available Povidone-iodine solution only, do
skin wash, or on viruses iodine not use acidified iodine solutions.
angling or other
delicate
equipment.
Treatment of Bacteria, fungi, 30 mg/L available Treat with thiosulphate before

water viruses iodine, left for 12 release.
hours
Calcium oxide Earthen-based All pathogens 0.5 kg/m2 for 1 month Repeat dose on at least two
ponds occasions in wet areas or in
event of flooding.
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Sodium Treatment of All pathogens Applied as a mixture NaOH generally sold as pellets.
hydroxide concrete or with CaOH and Repeat dose on at least two
cracked surfaces Teepol occasions in wet areas or in
of appropriate event of flooding. May also be
materials used as a 0.2% solution as a
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cleaning agent for equipment.
Treatment of Viral pathogens on Applied as a solution Teepol (wetting agent) enhances
appropriate suitable surfaces of 20 g/L NaOH for penetration through soil and into
surfaces where >10 minutes concrete.
high organic
loading may be a
problem
Treatment of All pathogens

IN At a rate to achieve
waste water pH >12 for 24 hours.
A
Treatment of All pathogens 50% (wt/vol) solution Dose should achieve a pH of
waste slurries at a rate of 30 L/1000 >12. Treat for >4 days.
(high organic L of slurry
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matter).
Calcium Treatment of All pathogens 40% (wt/vol) solution Dose should achieve a pH of
hydroxide waste slurries at a rate of 60 L/1000 >12. Treat for > 4 days.
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(high organic L of slurry

matter)
Glutaralde- Treatment of All pathogens 2% (wt/vol) for 30 Available as concentrate solution

hyde small items or minutes.
those subject to
corrosion.
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Formalin Treatment of All pathogens 8% (vol/vol) for 30 Available as 40% solution

solution hard or porous minutes Dilute 1:12 for use. Use only in
surfaces, Foot well-ventilated areas.
baths.
Treatment of All pathogens 40 L formalin solution Must be distributed evenly

waste slurries per 1000 L (40%)
(high organic
matter).
Treatment of All pathogens 300 mL of commer- Completely fill pipeline with

pipelines or cial grade formalin disinfecting solution and leave for
sewage channels solution per 10 L of 24 hours
(in situ) water
31
Quaternary Use on skin or Some bacteria, some 1 mg/L for >1 minute Limited range of efficacy
ammonium delicate items viruses
compounds
Use on hard Some bacteria, some 2 mg/L for >15 Limited range of efficacy
surfaces viruses minutes
Heat Treatment of Most pathogens 60°C for 10 minutes;

waste water Category A viruses 70°C for 6 minutes;
and some bacteria 75°C for 5 minutes;
may be resistant 80°C for 4 minutes.
Treatment of Most pathogens Steam cleaning at Difficult to regulate, best used as

hard surfaces Category A viruses 115– 130°C for 5 an adjunct to other disinfection
and equipment and some bacteria minutes methods Especially suitable for
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may be resistant treatment of transport tanks.
Desiccation Earthen tanks Most pathogens Dry for >3 months at Drying period can be reduced if
and light an average tempera- combined with an appropriate
ture of >18°C chemical disinfectant. Use drying
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and sunlight as a general adjunct
to all disinfection if possible
UV light Treatment of Viruses, bacteria, >25 mJ/cm2 Requires pre-treatment with

waste water
Treatment of
water.
fungi
Myxosporidean
species spores.
IN >35 mJ/cm2
chemical precipitation or filtration.
Ozone Water treatment All pathogens 1 mg/L for >1 minute

A
Disinfection of effluent water
Chlorination is the safest method of disinfection of water, rearing tanks and implements as the
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chlorine gas gets volatilized sooner or later.
Prior to chlorination the water should be passed through a filter capable of removing sus-
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pended organic material. Following is the protocol recommended for chlorination:
❖ Add 1.6 ml of Sodium hypochlorite (12.5% available chlorine) per litre of water collected in a vessel.
❖ Before the treatment commences, the chlorinated effluent should be brought to pH between
5.0 and 7.0.
❖ Subsequent to the addition of hypochlorite, waste water must be agitated for 10 min to ensure
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thorough mixing of hypochlorite and retained for a period of 1h.

❖ After the retention period, the chlorine in the waste water is neutralized by adding sodium
thiosulphate at a rate of 1.25g (2.5 ml of 50% sodium thiosulphate solution) per litre of treated
waste water.
❖ Agitated for 10 min before discharge.
Chlorinated water should not be discharged directly into adjacent waterways with out de-
chlorination.
Disposal of dead fishes

All dead animals or eggs should be kept in a solution of 10% formalin for a minimum of 5 days
before disposing off. The ratio of the volume of dead fish or fish eggs to solution should not be less
than 1:5.
32
Reference
Anonymous, 2008. Department of Agriculture, Fisheries and Forestry - Operational Procedures
Manual — Decontamination (Version 1.0). In: Australian Aquatic Veterinary Emergency Plan
(AQUAVETPLAN), Australian Government Department of Agriculture, Fisheries and Forestry,
Canberra, ACT. http://www.daff.gov.au/aquavetplan.
Burka, J F., Hammell, K. L., Horsberg, T. E., Johnsons, G. R., Rainnie, D. J. and Spears, D. J.
1997. Drugs in salmonids aquaculture – A review. Journal of Veterinary Pharmacology and
Therapeutics. 20: 333-349.
ANNEXURE 7
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Dispatch of sick fishes and representative samples for further examination
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* Lightner (1996). A Handbook of Pathology and Diagnostic Procedures for Diseases of Penaeid
Shrimp, World Aquaculture Society, Chapter 2
IN
The sick fish can be transported to the diagnostic laboratory by following ways:
Live fish: The fish should be packed in double plastic bags, filled with water to one third of
their capacity with the remaining 2/3 volume filled with air/oxygen. The bags should be tightly
sealed and packed inside a Styrofoam box or cardboard box lined with Styrofoam.
A
Whole dead fish, iced: Sick fishes or the ones that have just died should be placed each in
separate plastic bag and sealed. They are transported on crushed ice in a cooler or styrofoam
lined shipping box and should reach the laboratory within 24 hours. This could be the most suit-
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able sample for pathogen isolation and identification.
Whole dead fish for post mortem analysis: Sick or recently dead fish can be frozen and
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transported for post mortem analysis. Place each fish in an individual plastic bag, freeze (-20),
place in a shipping Styrofoam container, fill the bag with crushed ice and tightly seal air tight and
transport. Samples shall be collected from such samples for detecting viral pathogens.
Fishes for histopathology: Fishes to be used for histopathology should be sacrificed by

either immersing in crushed ice (small fishes) or by giving a hard blow on head in the case of larger
M
fishes. Small fishes should be ventrally split open and immersed in Davidson’s Fixative (Lightner,
1996)* having the following composition in a ratio of 10:1 (Fixative to fish).
For larger fish, the full length of the body cavity should be split open and the viscera and swim
bladder gently displaced to permit incision of each major organ, at least once, to allow maximum
penetration of the fixative. Ideally, the organ, or any lesions under investigation, should be re-
moved, cut into blocks and placed in a volume of fixative at least 10 times the volume of the tissue.
For skin preparation, it will be best to cut out several large pieces with a scalpel, briefly soaked
in the fixative cut into smaller sections about 1.0 cm wide and return the pieces quickly to fixative
for 24 hrs.
For samples from lesions, it is advisable to cut out a sample which includes healthy tissue
33
surrounding the lesion to allow for comparison between healthy and affected tissues, with a width
of no more than 1.0 cm and immediately placed in the fixative for 24 hrs.
The container with the fixative and tissue must be tightly sealed. Use plastic containers. The
sealed container should be placed in a shipping container filled with Styrofoam pellets or other
packing material.
Davidson’s fixative – Composition
(for 100 ml)
Ingredients Quantity (ml)
IA
95% Ethyl alcohol 33.0
100% Formalin 22.0
Glacial acetic acid 11.5
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Distilled water 33.5
Store in a cool place (<250C)
IN
ANNEXURE 8
Biopsy techniques
(Source : Noga, E.J., 2000., Fish Disease: Diagnosis and Treatment. Iowa University Press.
pp. 17-26)
A
Steps involved in biopsy
❖ Clean a bench or suitable working surface and, set up microscope ensuring all compo-
D
nents of it are working correctly.

❖ Place a smooth, non-absorbent surface such as laminated wood or plastic onto the bench
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and clean it using an antibacterial cleaner.

❖ Onto this place a moistened paper towel long enough for the fish to be placed on.
❖ Get a new clean slide and cover slip to hand
❖ A bath must be prepared to hold the fish before the actual sample is taken.
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❖ To prepare a bath, take a vessel suitable for the fish’s size, and place enough pond/
aquarium water into it so that the fish will be comfortable.
❖ Use plastic gloves to protect the fish during handling.
❖ If only one fish is showing disease signs, remove it from the population using a soft mesh
giving minimal amount of stress possible.
❖ On the other hand, if a population of individuals is affected, select the most affected fish or
that with the most obvious clinical signs.
❖ They are placed in tubs with sufficient quantity of water and anaesthetized.
❖ Meanwhile fresh water is kept in another tub aerated for recovery of the fishes after the
collection of samples for observation.
34
Anaesthetizing fishes using clove oil: Clove oil is diluted with ethanol at a ratio of 1:9 (clove oil:
ethanol) to yield a working stock solution of 100 mg/ml since each ml of clove oil contains approxi-
mately 1 gram of drug. Keep this solution in a dark bottle, preferably also in the dark. Concentra-
tions between 40 and 120 mg/liter are effective in freshwater fishes.
Skin biopsy
❖ Two methods are employed: skin scraping and fin clipping.
❖ Wear plastic gloves, gently lift the fish out of the water, and place it onto the wet towel.
❖ Skin scraping is performed using a scalpel blade gently scraping along the side of the
body or fins.
❖ Do not scrape too heavily, as it may damage the fish. Return the fish to the holding bath.
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❖ Once the smear is on the slide, a drop of filtered fresh water in the case of fresh water
fishes, brackish water in the case of brackish water fishes or sea water in the case of
marine fishes is added to the smear, and a cover slip placed over.
D
❖ Tap water should not be used as the chlorine will kill the parasites before they may have
been observed.
❖
❖
❖
IN
For placing correctly the cover slip, hold it by the sides at 45 0 angle, support it with a
dissection needle and lower it over the smear.
By this way trapping of air bubble with in the cover slip can be avoided.
Wipe out the overflowing fluid from the slide with paper towel without disturbing the cover
slip, and then place it on the stage of the microscope.
A
❖ The preparation should be examined immediately as the fish parasites may not live long
on the slide, and the smear may get dried shortly.
D
❖ However, the slide can be stored for short periods in a humidified container to prevent it
from drying out.
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❖ To make a humidified container simply place some wet paper towel into a sealable
plastic food container.
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35
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Fig 1: Steps involved in skin scraping
D
IN
A
D
Fig 2: Wet mount of skin scraping Fig 3: Wet mount of normal mucus
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In fin biopsy, the fin is spread and a scissor is used to cut a triangular wedge of fin tissue from
between the fin rays.
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Fig 4: Fin clipping and wet mount
36
Gill biopsy
❖ Immediately before biopsy, the gills should be examined grossly.
❖ Healthy gills are bright red. Pale pink gills indicate anemia, while brown gills suggest
methemoglobin formation.
❖ Gills quickly become pale pink soon after death because of the drainage of blood from the
gills.
❖ Gill biopsy is performed by inserting the tip of a pair of fine scissors into the gill chamber.
❖ The scissors are then gently opened, lifting the operculum until the gill arches can be
IA
seen.
❖ The tips of several primary lamellae are then cut and transferred to a slide; cover slip is
then applied.
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Only the tips of the lamellae should be cut; bleeding should be minimal.
IN
A
D
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37
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D
IN
A
D
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Fig 5: Steps involved in gill biopsy
38
ANNEXURE - 9
REQUIREMENTS IN AUTOPSY & BIOPSY SECTION
❖ Dissection set
❖ Magnifying lens
❖ Tissue paper
❖ Microscopic slides and cover slips
❖ Trays
❖ Data entry register
IA
❖ Carboy with 10% formalin for disposal of fishes
❖ Sufficient plastic covers, rubber bands and label slips
❖ Refrigerator with freezer chest
D
❖ Weighing balance
❖ Containers for holding fish
❖
❖
Anaesthetics
Gloves
IN
ANNEXURE-10
A
REQUIREMENTS IN WATER QUALITY ANALYSIS SECTION
❖ Colorimeter
❖
D
Burettes with burette stand

❖ Pipettes
❖
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Conical flasks
❖ Measuring cylinders
❖ Beakers
❖ Pipette bulbs
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❖ Plastic and enamel trays

❖ Pipette stand
❖ Sink
❖ Water still
❖ Distilled water containers
❖ Wash bottles
❖ Reagent bottles
❖ Refrigerator for storage of reagents
39
ANNEXURE 11
Documentation and maintenance of data
Documents should be maintained at each facility to record the following information:
a. Collection / procurement (Log book format – Annexure 11 A)

i. Name of the water body, location, site, date and time of fish collected/purchased
ii. Physical observation of the water quality
iii. Fishing methods adopted for collection
IA
iv. Species, Size, sex (if discernable)
v. Associated species caught and released
vi. Quantity/number (Catch Per Unit Effort- the gear operated, no of times it is operated and
D
the catch per operation)
vii. Quota/restriction, if any
b. Handling and transportation
i.
ii.
IN
Physical observation of the health status
Type of packaging materials used, type of packing used
iii. Details of aeration/oxygenation,
A
iv. Packing density/ tranquilizers used if any
v. Mode, time and duration of transportation.
D
vi. Temperature at which the fish is transported and the method adopted for regulating the
same.
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c. Primary Holding (Log book format – Annexure 11 B)

i. Green Certification Number of the supplier/society
ii. Mortality rate at the time of arrival
iii. Species, number, size received and sold
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iv. Health status (weak/active/injured), packing water quality (turbid/clear/frothy; smell of the
packing water)
v. Method of release- treatments used
vi. Details of holding facility, source and quality of water used
vii. Details of the chemicals used in the holding facility, if any.
viii. Duration of holding/ feed given, if any
ix. Mortality in the holding facility
x. Date of receiving and dispatch.
xi. Manner of disposal of dead fish
40
xii. Occurrence of disease out break if any and the details of treatment.
xiii. Disposal of used water and treatment if any.
d. Secondary Holding (Log book format – Annexure 11 C)
i. Green Certification Number of the supplier/society
ii. Name of the water body, location, site, date and time of fish collected/purchased
iii. Mortality rate at the time of arrival
iv. Health status (weak/active/injured), packing water quality (turbid/clear/frothy; smell of the
packing water)
v. Method of release, acclimatization and treatments used
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vi. Details of holding facility, source and type of water used
vii. Species, number, size received and sold
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viii. Details of the chemicals used in the holding facility, if any.
ix. Duration of holding/ feed given
x. Date of receiving and dispatch.
IN
xi. water quality (temperature, colour and odour, Nitrite, Oxygen, Hardness)
xii. Mortality in the holding facility
xiii. Manner of disposal of dead fish
A
xiv. Nets and other implements used.
xv. Occurrence of disease out break, if any and the details of
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treatment.
xvi. Disposal of used water and treatment if any
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xvii. Method of packaging and transportation used.

xviii.Packing density, chemicals used.
e. Exporter’s facility (Log book format – Annexure 11 D)
i. Green Certification Number of the supplier (primary and secondary holding facility)
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ii. Name of the water body, location, site, date and time of fish collected/purchased
iii. Fishing methods adopted for collection
iv. Mortality rate at the time of arrival
v. Method, duration, treatment and water quality of quarantine.
vi. Health status (weak/active/injured), packing water quality (turbid/clear/frothy; smell of the
packing water)
vii. Method of release, acclimatization and treatments used
viii. Details of facility including quarantine, source and type of water used
ix. Species, number, size received and sold
41
x. Details of the chemicals used in the holding facility, if any.

xi. Duration of holding/ feed given
xii. Date of receiving and dispatch.
xiii. water quality (temperature, colour and odour, Nitrite, Oxygen, Hardness)
xiv. Mortality in the holding facility
xv. Manner of disposal of dead fish
xvi. Nets and other implements used.
xvii. Occurrence of disease out break if any and the details of treatment.
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xviii.Disposal of used water and treatment if any
xix. Method of packaging and transportation used.
xx. Packing density, chemicals used,
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xxi. Details of health certificate obtained for the consignment.
xxii. Requirements of the buyer including health and other standards stipulated.
f.
i.
ii.
IN
Breeding and Culture facility (Log book format – Annexure 11 E)
Site, location & address of the facility
Green Certification Number of the unit
iii. Source of brood stock/fish - Name of the water body, location, site, date and time of
A
collection/purchase, species wise number of fishes collected/purchased.
iv. Fishing methods adopted for collection, if from wild.
D
v. Mortality rate at the time of arrival.

vi. Health status (weak/active/injured), packing water quality (turbid/clear/frothy; smell of the
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packing water).
vii. Method of release, acclimatization and treatments used.
viii. Details of holding facility - source and type of water used, Number and type of ponds/
tanks, capacity.
ix. Species, number, size received and sold.
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x. Details of the chemicals used in the facility, if any (Name, dosage, time & frequency of
usage).
xi. Daily stock of brood stock/fishes available in the unit.
1. Species wise, variety wise and sex wise number of brood stock maintained.
2. Details of hormones used – type, dosage, frequency and mode of administration.
3. Details of chemicals used for sanitation/ disinfection purpose – type, dosage, fre-
quency and mode of administration.
4. Spawning schedule - species wise.
42
5. Hatching details (Species wise & variety wise hatching and survival rates).
6. Sex reversal techniques used, if any – hormones/chemicals- type, dosage, frequency
and mode of administration.
7. Water quality parameters – in hatching, nursery and rearing tanks/ponds.
8. Species wise survival in hatching, nursery and rearing tanks/ponds.
9. Details of feeds used in hatching, nursery and rearing stages – quantity & feeding
schedule.
xii. water quality in the culture facilities (temperature, colour and odour, Nitrite, Oxygen, Hard-
ness, ammonia).
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xiii. Stocking density in the culture system- species & variety wise.
xiv. Feeds & feeding schedules - species & variety wise.
xv. Duration rearing & harvesting details.
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xvi. Mortality in the culture system.
xvii. Manner of disposal of dead fish.
xviii.Nets and other implements used.
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xix. Occurrence of disease out break, if any and the details of treatment.
xx. Disposal of used water and treatment.
xxi. Method of handling, packaging and transportation adopted.
A
xxii. Packing density, chemicals used.
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ANNEXURE 11 A
LOG BOOK FORMAT
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I. Collection/procurement
1. Date & time :
2. Location (address):
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3. Water quality : Clear Transluscent Turbid
4. Fishing method : Drag net Cast net Dip net Others(Specify)
43
5. Fish caught :
Sl no Species Number Size Sex
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D
6. Associated species caught and released:
IN
7. Quantity of the catch ( No.s):
A
8. No of times the gear operated:
D
9. CPUE (Catch per unit effort):
10. Quota restriction, if any:

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II. Handling & Transportation
1. Health status : Healthy Weak Moribund Dead

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2. Packaging material used: Polythene bags Water tanks Others (specify)
44
3. Aeration provided : Yes / No
4. Packing density (Nos/litre)
5. Transportation details:
Mode : Road / Rail / Others (specify)
Time : Morning / Noon / Evening / Night
Duration (In Hrs):
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6. Temperature at which fish is transported:
7. Method adopted for regulating temperature:
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Name :
Signature:
Designation:
IN
ANNEXURE 11 B
A
LOG BOOK FORMAT
Primary Holding facility
D
1. Date & time :
2. Green certification number of the supplier/society:

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3. Mortality at arrival (% of the total) :
4. Details of receipt and sale of fishes:
Sl no Species No. Size Sex Sold (Nos.)

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45
5. Health status : Active / Weak / Injured / Dead
6. Packing water quality : Clear / turbid / frothy / foul smelling / No smell
7. Source of water: Open well / bore well / river / others (specify)
8. Quality of water : Temp: pH:
9. Holding facility
a) Chemicals used if any:
b) Duration of holding
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c) Feed given if any
d) Mortality rate:
10. Date of dispatch:
D
11. Disease out break if any:
(Name of the disease)
12.
13.
Treatments given :
Disposal of used water:

IN
After treatment / without treatment / to open areas / drainage
channels
A
14. Details of treatment :
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Name :
Signature:
Designation:
46
ANNEXURE 11 C
LOG BOOK FORMAT
Secondary Holding facility
1. Date & time :
2. Green certification number of the supplier/society:
3. Mortality at arrival (% of the total) :
IA
4. Details of receipt and sale of fishes:
Sl no Species No. Size Sex Sold (Nos.)
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IN
A
D

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7. Method of release, acclimatization & treatments:
a) Release:
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b) Acclimatization
c) Treatments:
9. Water quality :
a) Temperature: b) Colour :
c) Odour : d) Nitrite :
e) Hardness : f) Oxygen : g) Ammonia :
47
10. Holding facility
b) Duration of holding:
c) Feed given if any:
d) Mortality rate:
12. Disease outbreak if any:

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13. Treatments given :
14. Disposal of used water: After treatment / without treatment / to open areas / drainage
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channels
16.
17.
18.
Nets and other implements used:
IN
Disposal of dead fish : Incinerated / buried / others (specify)
Packaging method
A
20. Chemicals used in the packing water:

D
PE
M
Name :
Signature:
Designation:
48
ANNEXURE 11 D
LOG BOOK FORMAT
Exporting facility (Green certification No:)
1. Date & time :
2. Green certification number of :
a. Primary holding facility:
b. Secondary holding facility:
IA
3. Source of the fish
a. Name of water body, location & site
D
b. Date & time of fish collected /purchased:
c. Fishing methods adopted for collection:
4. Mortality at arrival (% of the total) : IN

A
5. Quarantine details:
D
a. Method:
b. Duration (Days / hrs)

PE
c. Treatments given
d. Water quality:
Temperature pH Hardness Oxygen Nitrite

M
49
a) Release:
b) Acclimatization
c) Treatments:
10. Water quality :
IA
a) Temperature: b) Colour :
c) Odour : d) Nitrite :
D
e) Hardness : f) Oxygen : g) Ammonia :
11. Holding facility
b) Duration of holding:
IN
c) Feed given if any:
A
d) Mortality rate:
D
12. Receipt and sale of fishes:
Sl no Species No. Size Sex Sold/exported

PE
(Nos.)
M
50

15. Treatments given:
channels
18. Nets and other implements used:
19. Disposal of dead fish : Incinerated / buried / others (specify)
IA
20. Packaging method
D
23. Details of health certificate obtained
Sl
no
Certificate No
and date
IN
Issued by (Name of the
competent authority)
Country of
destination
1
A
2
D
3
PE
24. Requirements of the buyer:

M
Name :
Signature:
Designation:
51
ANNEXURE 11 E
LOG BOOK FORMAT
Breeding & culture facility (Green certification No:)
1. Location and address :
2. Source of brood stock
a. Name of water body, location & site
b. Date & time of fish collected /purchased:
IA
c. Fishing methods adopted for collection if from wild:
3. Fish collected / purchased:
D
Sl. No Name of species Nos.
IN
A
D
PE
4. Mortality at arrival (% of the total):
5. Health status: Active / Weak / Injured / Dead
6. Packing water quality: Clear / turbid / frothy / foul smelling / No smell

M
a) Release:
b) Acclimatization
c) Treatments:
52
8. Holding facility
a) Source of water: Open well / bore well / river / others (specify)
b) Ponds & tanks
Item Numbers Type

present
Earthen Cement Glass FRP Others (specify)
PONDS
IA
TANKS
D
9. Receipt and sale of fishes:
Sl no Species No. Size Sex Sold/exported
IN (Nos.)
A
D
PE
10. Chemicals used in the tanks/farm

M
SL no Name of the chemical Dosage Time of Frequency of

application application
53
11. Daily stock of brood stock & other fishes: - IN STOCK REGISTER
STOCK REGISTER FOR FISHES / BROOD STOCK (FORMAT)
Date Name of the fishes Variety Sex No. remaining
IA
D
12. Hormones used
SL no Name of the hormone IN Dosage Mode of

application
Frequency of
application
A
D
13. Chemicals used for Disinfection/ sanitation

PE
SL no Name of the hormone Dosage Mode of Frequency of

application application
M
14. Spawning schedule
SL no Name of the Fish Variety Date/Time of spawning
54
15. Hatching details
SL no Name of the fish Variety Approx.no Survival rate at

of larvae juvenile stage
16. Sex reversal techniques, if any
IA
SL no Name of the Type Dosage Frequency Mode of
hormones/chemicals administration
D
17. Water quality:
IN
Section Temperature pH Hardness Oxygen Nitrite Ammonia
A
Hatching
D
tanks
Nursery
tanks
PE
Rearing
tanks
18. Feeds & feeding schedules

M
a) Section wise
Section Quantity Feeding schedule
Hatching tanks
Nursery tanks
Rearing tanks
55
b) Species wise
Name of species Variety Quantity Feeding schedule
IA
19. Rearing & harvesting details:
Name of Harvesting details
D
species Rearing period
Method Time No
IN
A
20. Mortality in the culture system:
D
Section Mortality (%) Reason
Hatching tanks
PE
Nursery tanks
Rearing tanks
M

22. Treatments given:
channels
56
24. Details of treatment of the water :
25. Nets and other implements used:
26. Disposal of dead fish: Incinerated / buried / others (specify)
27. Packaging method:
28. Transportation method : Road / rail / air / others
29. Packing density (No/litre)
IA
D
IN
A
D
PE
Name :
Signature:
Designation:
M
57
ANNEXURE 12 A
SCORE CARD FOR COLLECTION SITE

Sl.no Major attribute Assessment Score Attributes Score
criteria obtained
1 Environmental Excellent 10 a) Sustainable collection –

integrity ( 10) 3marks
b) Eco-friendly gear & meth-
ods-3marks
c) Collection from permitted
IA
waters-2marks
d) Pollution free environment-
1mark
e) Time of collection-1mark
D
Very good 8
Good
Fair
IN 6
Poor 2
A
2 Social integrity Excellent 5 a) Harmony with local
community – 2marks
D
b) Proper water treatment

before discharge-2mark
c) Proper disposal of dead
PE
fish-1 mark
Very good 4
Good 3
M
Fair 2
Poor 1
3 Infrastructure Excellent 30 a) Oxygen cylinder – 5

b) Packing bag – 5marks
c) Good quality water-
5marks
d) Holding facility -10marks
e) Appropriate handling &
transportation-5 marks
58
Very good 25
Good 20
Fair 10
Poor 5
4 Documentation Excellent 10 a) log book (as per annexure

11A) - 5
b) Sales register-3
IA
c) Stock register-2
Very good 8
D
Good 6
Fair 4
5 Water quality
Poor
Excellent
IN 2
15 a) Oxygen > 5ppm –5marks

b) Ammonia < 0.02 ppm-
5marks
A
c) Clear water-3marks
d) Odourless -2marks
D
Very good 12
Good 8
PE
Fair 3
Poor 2
6 Human re- Excellent 10 a) Collection/fishing skill -

M
sources 5marks
b) Handling skill-2marks
c) Packing skill-2marks
d) Transportation skill-1mark
Very good 8
Good 6
Fair 4
Poor 2
59
7 Handling & Excellent 10 a) Handling with appropriate

transportation gadgets – 2marks
b) Mortality rate less than
10% - 2 marks
c) Appropriate packing
density -2 marks
d) Use of aerators -2marks
e) Stress free transportation
– 2 marks
Very good 8
IA
Good 6
Fair 4
D
Poor 2
8 Biosecurity Excellent 10 a) Hygenic fishing operations

IN -3marks
b) Pollution & disease free
collection site - 3marks
c) Hygenic collection &han-
dling gadgets-2marks
A
d) Absence of clinical symp-
toms of disease- 2marks
D
Very good 8
Good 6
PE
Fair 4
Poor 2
M
60
ANNEXURE 12 B
SCORE CARD - 2 (PRIMARY HOLDING FACILITY)

criteria obtained
1 Environmental Excellent 10 a) Fish purchase from Green

integrity ( 10) certified supplier/collector –
5marks
b) Proper discharge of effluent
water-3marks
IA
c) Proximity to the collection
site- 2marks
Very good 8
D
Good 6
Fair
Poor
IN 4
2 Social integrity Excellent 5 a) Harmony with local commu-

A
nity – 2marks
D

fish-1 mark
PE
Very good 4
Good 3
Fair 2
M
Poor 1
3 Infrastructure Excellent 30 a) Adequate number of

cement/FRP/Glass tanks -
10
b) Aeration & filtration facility -
5 marks
c) Adequate number of oxygen
cylinders – 10 marks
d) Sufficient number of packing
&handling materials -
5marks
61
Very good 25
Good 20
Fair 10
Poor 5
4 Documentation Excellent 10 a) log book (as per annexure

11B) - 5 marks
b) Purchase records -2marks
c) Sales register-2 marks
IA
c) Stock register-1 marks
Very good 8
D
Good 6
Fair 4
5 Water quality
Poor
Excellent
IN 2
15 a) Oxygen > 5ppm –5marks

b) Ammonia < 0.02 ppm-
5marks
A
c) Clear water-3marks
d) Odourless -2marks
D
Very good 12
Good 8
PE
Fair 3
Poor 2
6 Human re- Excellent 10 a) Fish maintenance skill – 5

M
sources marks
b) Handling skill-2marks
c) Packing skill-2marks
d) Transportation skill-1mark
Very good 8
Good 6
Fair 4
Poor 2
62
7 Handling & Excellent 10 a) Handling with appropriate

transportation gadgets – 2 marks
b) Mortality rate less than
10% - 2 marks
c)Appropriate packing
density - 2 marks
d) Use of aerators - 2 marks
e) Stress free transportation –
2 marks
Very good 8
IA
Good 6
Fair 4
D
Poor 2
8 Biosecurity Excellent 10 a) Hygienic fish handling and

IN proper handling gadgets-
3marks
b) Contamination, parasite &
disease free stock - 2
marks
A
c) Absence of clinical symp-
d) Treatment kits – 1 mark
D
e) Proper disposal f dead

fishes – 1 mark
f) Bird / insect control - 1
PE
mark
Very good 8
Good 6
M
Fair 4
Poor 2
63
ANNEXURE 12 C
SCORE CARD FOR SECONDARY HOLDING FACILITY

1 Major attribute Assessment Score Attributes Score
criteria obtained
Environmental Excellent 10 a) Fish purchase from Green

integrity ( 10) certified supplier/collector –
5marks
b) Proper discharge of effluent
water-3marks
IA
c) Flood safe site- 2marks
Very good 8
D
Good 6
Fair 4
2 Social integrity
Poor
Excellent
IN 2
5 a) Harmony with local commu-

nity – 2 marks
A
D
fish-1 mark
Very good 4
PE
Good 3
Fair 2
Poor 1
M
3 Infrastructure Excellent 30 a) Lay out and design com-

plies to requirements as per
Annexure 2 - 5 marks
b) Aeration & filtration facility -
3 marks
c) Adequate number of oxygen
cylinders – 3 marks
d) Sufficient number of packing
& handling materials - 4
marks
e) Laboratory facility – 3 marks
64
f) Acclimatization & quaran-

tine facility -5 marks
g) Disease treatment facility -
5 marks
h) Power backup facility – 2
marks
Very good 25
Good 20
IA
Fair 10
Poor 5
D
4 Documentation Excellent 10 a) log book (as per Annexure
11C) – 5 marks
b) Purchase records -1 mark
c) Sales register-1 mark
IN c )Stock register (consumable
and permanent) -1 mark
d) Document of daily sanita-
tion – 1 mark
e) Document of daily mainte-
A
nance - 1 mark
Very good 8
D
Good 6
PE
Fair 4
Poor 2
5 Water quality Excellent 15 a) Oxygen > 5ppm – 3 marks

b) Ammonia < 0.02 ppm- 3
M
marks
c) Nitrite <0.02 ppm-3 marks
d) Nitrate 50 mg/L – 2 marks
e) pH 6-8 – 1 mark
f) Odourless -1mark
g) Hardness as required for
the species - 1 mark
h) Ambient temperature – 1
mark
Very good 12
65
Good 8
Fair 3
Poor 2
6 Human re- Excellent 10 a) Presence of qualified

sources technician – 3 marks
b) Adequate number of skilled
staff – 2 marks
c) Handling skill-2marks
IA
d) Packing skill-2marks
e) Transportation skill-1mark
Very good 8
D
Good 6
Fair 4
7 Holding,
Poor
Excellent
IN 2
10 a) Standard feeding protocol –

Handling & 2 marks
A
transportation b) Mortality rate less than
10% - 4 marks c) Appro-
priate life support systems -
D
2 marks
d) Use of temperature con-
trolled vehicle for transpor-
PE
tation -2marks
e) Proper conditioning and
transportation – 2 marks
Very good 8
M
Good 6
Fair 4
Poor 2
8 Biosecurity Excellent 10 a) Quarantine measures -

3marks
b) Contamination, parasite &
disease free stock - 2
marks
66

e) Proper disposal of dead
fishes – 1 mark
f) Bird / insect control - 1
mark
Very good 8
Good 6
Fair 4
IA
Poor 2
D
IN
A
D
PE
M
67
ANNEXURE 12 D
SCORE CARD FOR BREEDING & CULTURE FACILITY

criteria obtained
1 Environmental Excellent 15 a) Flood safe site- 3marks

integrity ( 15) b) Biological inputs(such as
brood stock, larvae, live
feed and aquatic plants)
sourced from Green certi-
IA
fied supplier/collector –
5marks
c) Absence of Genetically
Modified Organisms-1
D
marks
d) Compatible species in the
culture system-1 mark
IN e) Good waste water treatment
system-2 marks
f) Rain water harvesting
system-1 mark
g) Recirculating system-1 mark
A
f) Use of non conventional
energy sources (such as
solar energy, wind energy
D
and biogas)-1
Very good 12
PE
Good 9
Fair 6
Poor 3
M

nity – 2 marks
b) Non utilization of drinking
water from public distribu-
tion system-2marks
fish-1 mark
Very good 4
Good 3
68
Fair 2
Poor 1
3 Infrastructure Excellent 30 a) Lay out and design com-

plies to requirements as per
Annexure 5 -5 marks
b) Acclimatization & quaran-
tine facility -3 marks
c) Live feed/Fish feed prepara-
tion& storage area-2marks
IA
d) Aeration & filtration facility -
3 marks
e) Adequate life support
systems such as oxygen
D
cylinders, aerators, blowers
&filters – 3 marks
f) Laboratory facility – 3 marks
g) Disease Treatment facility -
IN 3 marks
marks
i) Pre shipment conditioning
facility - 2 marks
A
j) Office & accommodation/
rest room –4marks
D
Very good 25
Good 20
PE
Fair 10
Poor 5
4 Documentation Excellent 10 a) log book (as per Annexure

M
11E) – 5 marks
c) Stock register (consumable
d) Document of daily sanita-
tion & premises cleaning - 1
mark
e) Equipments maintenance
register -1mark
Very good 8
69
Good 6
Fair 4
Poor 2

b) Ammonia < 0.02 ppm- 2
marks
c) Nitrite <0.02 ppm-2 marks
d) Nitrate < 50 mg/L – 1 marks
IA
e) Optimum pH as required for
the species – 1 mark
f) Hardness as required for the
species - 1 mark
D
g) Temperature control at
packing facility 220C ± 2 – 1
mark
Very good
Good
IN 8
Fair 4
A
Poor 2
D
6 Human re- Excellent 10 a) Presence of qualified techni-

sources cian/s – 5 marks
b) Trained manpower for brood
PE
stock & breeding section – 3

marks
c) Trained manpower for farm
management -2marks
Very good 8
M
Good 6
Fair 4
Poor 2
7 Holding, Han- Excellent 10 a) Standard feeding protocol –

dling & trans- 2 marks
portation b) Mortality rate less than 10%
(Operations) - 2 marks
70
c) Proper signage and labels -

2
d) Operation schedules
(cleaning, water quality
checking & feeding) - 2
marks
trolled vehicle for transpor-
tation -2marks
Very good 8
IA
Good 6
Fair 4
D
Poor 2
8 Biosecurity Excellent 10 a) Quarantine measures -

IN 3marks
b) Parasite & disease free
stock - 1 mark
toms of disease- 1mark
A
fishes – 2 mark
D
f) Proper pre shipment

conditioning packing
&stacking - 2 mark
PE
Very good 8
Good 6
Fair 4
M
Poor 2
71
ANNEXURE 12 E
SCORE CARD FOR EXPORT UNIT

criteria obtained
1 Environmental Excellent 15 a) Flood safe site- 2marks

integrity ( 15) b) Biological inputs(such as
brood stock, larvae, live
feed and aquatic plants)
sourced from Green certi-
IA
fied supplier/collector –
5marks
c) Absence of Genetically
Modified Organisms-1
D
marks
d) Good waste water treatment
system-2 marks
IN e) Rain water harvesting
system-1 mark
f) Recirculation system-1 mark
g) Use of non conventional
energy sources (such as
A
solar energy, wind energy
and biogas)-1mark
h) Use of eco friendly materials
D
and green buildings -2mark
Very good 12
PE
Good 9
Fair 6
Poor 3
M

(5) nity – 2 marks
b) Non utilization of drinking
water from public distribu-
tion system-2marks
fish-1 mark
Very good 4
Good 3
72
Fair 2
Poor 1
3 Infrastructure Excellent 30 a) Lay out and design complies

(30) to requirements as per
Annexure 4 -5 marks
b) Acclimatization & quarantine
facility -3 marks
c) Live feed/Fish feed prepara-
tion& storage area-2marks
IA
d) Aeration & filtration facility -3
marks
e) Adequate life support
systems such as oxygen
D
cylinders, aerators, blowers
&filters – 3 marks
f) Laboratory facility – 3 marks
IN g) Disease Treatment facility -

3 marks
marks
i) Pre shipment conditioning
facility - 2 marks
A
j) Office & accommodation/
rest room –4marks
D
Very good 25
Good 20
PE
Fair 10
Poor 5
4 Documentation Excellent 10 a) Log book (as per Annexure

M
(10) 11D) – 4 marks

c) Stock register (consumable
d) Document of daily sanitation
& premises cleaning - 1 mark
e) Equipments maintenance
register -1mark
f) Documents to prove rejec-
tion free export for the
preceding 1 year-1mark
73
Very good 8
Good 6
Fair 4
Poor 2

(10) b) Ammonia < 0.02 ppm- 2 marks
8 c) Nitrite <0.02 ppm-2 marks
IA
d) Nitrate < 50 mg/L – 1 marks
6 e) Optimum pH as required for
the species – 1 mark
4 f) Hardness as required for the
D
species - 1 mark
2 g) Temperature control at
packing facility 220C ± 2 – 1
mark
Very good
Good
IN 10
A
Fair
Poor
D
6 Human re- Excellent 8 a) Presence of qualified

sources technician/s – 5 marks
PE
b) Trained manpower at all

sections - 3
c) Qualified managerial
personnel for export market-
ing-2marks
M
Very good 6
Good 4
Fair 2
Poor
7 Holding, Excellent 10 a) Standard feeding protocol –

Handling & 2 marks
transportation b) Mortality rate less than 10%
(Operations) - 2 marks
74
c) Proper signage and labels -

2
d) Operation schedules
(cleaning, water quality
checking & feeding) - 2
marks
trolled vehicle for transporta-
tion -2marks
Very good 8
IA
Good 6
Fair 4
D
Poor 2
8 Biosecurity Excellent
IN 10 a) Quarantine measures -
3marks
b) Parasite & disease free
stock - 1 mark
toms of disease- 1mark
A
fishes – 2 mark
D
f) Proper pre shipment condi-

tioning packing &stacking -
2 mark
PE
Very good 8
Good 6
Fair 4
M
Poor 2
75
ANNEXURE – 13
Guidelines for the import of ornamental fishes to India
The information furnished here are excerpts from the NBFGR Documents on Aquatic Exot-
ics and Quarantine Guidelines & National Strategic Plan. The readers are advised to refer to
the original document for more details.
Citation:
Ponniah, A. G. Unnithan, V. K. & Sood, N. (2002) National Strategic Plan for Aquatic Exotics and
Quarantine. NBFGR Special Publication No. 3, xiii + 119p. National Bureau of Fish Genetic
Resources, Lucknow, U.P., India.
IA
Ponniah, A. G. & Sood, N. (2002) Aquatic exotics and quarantine guidelines. NBFGR Special
Publication No. 4, xii + 97p. National Bureau of Fish Genetic Resources, Lucknow, U.P., India.
Proforma for submitting proposals for introduction of live aquatic organisms
D
1 Name of the applicant :
2 Status of the applicant :
2.1
2.2
2.3
Central Govt. Department/
State Govt./ Private
Govt. Research Institute/ Academic
Institution/ private research laboratory
Designation
IN :
:
:
2.4 Full Address
A
Telephone
Fax
D
E-mail
3.1 Brief field experience of the

applicant with activities related to
PE
species being introduced :

4.1 Name of the aquatic organism proposed
to be introduced in Indian waters :
4.2 Type of organism
Plant/ mollusc/ crustacean/ fish/ others
(please specify) :
4.3 Scientific name (s) :
M
4.4 Common names used in the country from

where it is imported :
4.5 Source of origin (Wild/cultured) :
5 Purpose of introduction :
5.1 Research/Aquaculture/Aquarium trade :
5.2 Any additional or specific information on scope
and extent of activities with the organism
proposed to be imported :
5.3 Any technical knowhow that will be transferred
from the importing country along with the
organism :
6 Import details :
6.1 Life stages
Unfertilized eggs/ cryopreserved milt develop-
ing eggs/ larvae/ juveniles/ immature adult/
brood stocks :
76
6.2 Quantity/ size of import :
6.2.1 Number :
6.2.2 Average weight (g) :
6.2.3 Average length (cm) :
7 Biological profile of original species as
recorded from wild :
7.1 Maximum size (cm) with likely age :
7.2 Maximum weight (g) with likely age :
7.3 Adult habitat :
7.3.1 Freshwater/ brackish water/ marine :
7.3.2 Anadromous/ Catadromous :
7.3.3 Flowing/ stagnant :
7.3.4 Usually found in bottom/ column/ surface of
water :
7.3.5 Littoral/ deeper waters :
IA
7.4 Temperature requirement for different stages :
7.4.1 Yolk sac larvae :
7.4.2 Post larvae :
7.4.3 Fry :
7.4.4 Fingerlings :
D
7.4.5 Adults :
7.5 If migratory in nature :
7.5.1 Feeding/ breeding migration :
7.5.2 Life stages undergoing migration (broodstock/
7.6
7.6.1
7.6.2
7.6.3
juvenile/ larval).
Breeding
Bisexual/ hermaphrodite/ clonal
Oviparous/ viviparous
Age at maturity
IN :
:
:
:
:
7.6.4 Period/ time of breeding :
A
7.6.5 Fecundity :
7.6.6 Temperature requirements :
7.6.7 Salinity requirements :
7.6.8 Other requirements :
D
7.6.9 Feeding habits (herbivorous/ carnivorous/

omnivorous :
7.6.10 Natural food item of
- Larval stages
PE
- Juvenile
- Adult :
8 In its natural habitat with which species it is
known to compete for :
8.1 Food :
8.2 Breeding grounds :
8.3 Same ecological niche :
M
9 Genetic profile :
9.1 Wild/ captive/ domesticated/ developed :
9.1.1 If wild, area of collection :
9.1.2 If captive - Number of
generation held in hatchery :
9.1.3 If domesticated - Number of
generations undergone domestication :
9.1.4 If developed - Originals/ stocks
used for development :
9.1.5 Genetic mechanism used for developing
(Selection/ hybridization/ chromosome
engineering/ genetic engineering/ combined
(please specify) :
9.1.6 Strain
No./ Name :
77
9.1.7 Genetic stability of characters developed :
10 Quarantine profile :
10.1 Parasites and other diseases recorded, if any,
in wild :
10.2 Parasites and other disease, recorded in
farmed stocks :
10.3 a). Has any OIE/ NACA or list of diseases of
concern to India been reported from exporting
country (Annexure 14 & 15) :
b). If yes, from which species. :
10.4 Has it been reported from candidate species
from exporting country. :
10.5 a). Has there been any disease outbreak in
the farm from where import is proposed. :
b). If yes so, give details. :
IA
10.6 a). Is there any active surveillance in the
exporting country. :
b). Does the particular hatchery/ farm from
where the species is imported is covered by a
national aquatic health surveillance system. :
D
c). Has this particular hatchery or farm been
declared free of any disease (if yes, specify
disease(s) :
10.7 Name and designation of the officer who
11
11.1
would sign pre movement certificate
Details of earlier export of the same
species from the farm/ hatchery from
where import is planned
IN
The date and details (listed in item 5, 6) with
:
address of organization to which earlier

A
exports have been sent :
11.2 Any evaluation done in the importing countries
on ecological and quarantine angle before
import. If so, address of agency and copy of
D
report :
11.3 Is pre-shipment quarantine possible from
exporting country? If so, type of quarantine
and certification :
PE
11.4 Route, packaging and shipment details :

11.5 Any facility with the importer for maintaining
imported stock in isolation for a minimum of 30
days. :
12 Recommendations of the State Govern-
ment in case of Private Company :
13 Other relevant information concerning the
M
proposed project :
13.1 Proposed period of import :
13.2 Experimental area
- Hatchery/ farm location where brood stock
developed from import will be maintained
- Address
- Names of nearby stream, canals, rivers,
lakes and distance from the farm :
13.3 Operational areas :
Progeny produced from brood stock will be
sold as live/ killed :
For export/ only within India :
If within India which States :
13.4 Liaison with State/ Central Govt. :
13.5 Liaison with any research organization which
could oversee import :
78
14 Information regarding farm where imported
aquatic organisms will be maintained :
14.1 What is the distance in kilometres from the
nearest water body? :
14.2 What is the height of the farm from sea level? :
14.3 Has the farm been flooded over the last 10
years? If yes, state the year of flooding. :
14.4 Whether the water supply is independent? :
14.5 Whether there is a provision for preventing
backflow? :
14.6 Whether the discharge is going to the natural
water bodies or not? If yes does it have a
system of filters? :
14.7 Whether the farm is fenced? :
14.8 Whether nets cover the pond? :
IA
14.9 Whether the farm will be used only for exotic
aquatic organisms or other aquatic organisms
as well? :
14.10 Whether the capacity for emergency harvest
or destroying the whole stock is there? :
D
14.11 Experience in aquaculture sector (in years) :
14.12 Other related activities in aquaculture sector :
Date
IN Signature
A
Seal
D
Essential enclosures :
1. Photos of organisms to be imported
2. Layout of hatchery/ farm (item 13.2)
PE
3. Scientific literature/ documents for items 7, 8, 9, 10 (except those marked

in boxes *)
4. Documents/ letter for items 11 and 12.4, 12.5
Instructions for filling proforma
M
1. For each species separate proforma may be used.

2. No column should be left blank. If information is not available, indicate by N.A. and if item is not relevant by
N.R.
3. Effort may be made to supply relevant information supported by scientific literature/ documents. This would facili-
tate rapid evaluation from exotic and quarantine angles. Due to inadequate information on items other than en-
closed in boxes request for import can be rejected since evaluation of risks will not be possible.
79
Proforma for evaluating ecological risks of fresh introductions of ornamental fish,

which have already been introduced in India
1.1 Name of the ornamental fish introduced in

Indian waters :
1.2 Scientific name (s) :
1.3.1 Name of countries where the species has

been introduced earlier
1.3.2 Name of country from which the species is

proposed to be introduced :
IA
1.3.3 Source of origin (wild /cultured) :
1.4 Common names used in the country from

where it is imported, and in India, (if any) :
D
2 Will the species be used for any purpose
other than aquarium trade :
3 Import details :
3.1
3.2
3.2.1
Life stages
Juveniles/ immature adult/ brood stocks
Quantity/ size of import
Number
IN :
:
:
3.2.2 Average weight (g) :
A
3.2.3 Average length (cm) :
4 Biological profile of original species as

recorded from wild :
D
4.1 Maximum size (cm) with likely age :
4.2 Maximum weight (g) with likely age :

PE
4.3 Adult habitat :
4.3.1 Freshwater/ brackish water/ marine :
4.3.2 Anadromous/ catadromous :

M
4.3.3 Flowing/ stagnant :
4.3.4 Usually found in bottom/ column/ surface of

water :
4.3.5 Littoral/ deeper waters :
4.4 Temperature requirement for different stages :
4.4.1 Yolk sac larvae - :
4.4.2 Post larval :
4.4.3 Fry :
80
4.4.4 Fingerling/ juvenile :
4.4.5 Adult :
4.5 If migratory in nature :
4.5.1 Feeding/ breeding migration :
4.5.2 Life stages undergoing migration (/ juvenile/

larvae/ broodstock /). :
4.6 Breeding :
4.6.1 Bisexual/ hermaphrodite/ clonal :
IA
4.6.2 Oviparous/ viviparous :
4.6.3 Age at maturity :
4.6.4 Period/ time of breeding :
D
4.6.5 Fecundity :
4.6.6 Temperature requirements :
4.6.7
4.6.8
Salinity requirements
Other requirements
IN :
4.6.9 Generation interval :

A
4.7 Feeding habits
(herbivorous/ carnivorous/ omnivorous)
D
4.7.1 Natural food items of
- Larvae :
PE
- Juvenile
- Adult
4.7.2 Artificial feeds accepted/ not accepted (If

accepted, what are the feeds?) :
M
5. In its natural habitat with which species it is

known to compete for :
5.1 Food
5.2 Breeding grounds :
81
5.3 The same ecological niche :
6. Genetic profile
6.1 If developed - Originals/ stocks used for

development :
6.2 Genetic mechanism used for developing

(selection/ hybridization/ chromosome
engineering/ genetic engineering/ combined
(please specify) :
6.3 Genetic stability of characters developed :
6.4 Morphological characters of parent strains in
IA
terms of body shape, colour and fin shape :
6.5 Morphological changes in body shape, colour

and fin shape in the present strain :
D
Strain No./Name Body shape Coloration Fin shape
1
2
3
4
IN
5
A
* A separate photograph for each strain to be enclosed
D
7 Earlier import
7.1 Year of first import and total number

imported, if available :
PE
7.2 Documentary evidence for Ist import :
7.3 Name and address of the firm which imported

it for the first time :
M
7.4 Area where introduced for the first time :
7.5 Were the imported ornamental fish
a) Confined to large secure farms :
b) Distributed to backyard hatcheries or

hatcheries without secure facilities :
8 New introductions
8.1 a) Will the ornamental fish be confined to

large secure farm :
82
b) Will the ornamental fish be distributed to

backyard hatcheries or hatcheries without
secure facilities :
8.2 Are the ornamental fish meant for
a) Export :
b) Internal market :
8.3 Whether the new introduction of ornamental

fish will be maintained by
a) One hatchery* :
IA
b) Many hatcheries* :
D
IN
Date Signature
A
Seal
D
Essential enclosures:
PE
1. Photos of organisms imported
2. *Address of Hatchery/ Farm
Instructions for filling proforma
1. For each species use a separate proforma.

M
2. No column should be left blank. If information is not available fill N.A. and if item is not relevant, N.R.
83
Proforma for ecological risk evaluation (Part 1-4) and disease risk evaluation (Part 5)
[Answer Yes (Y)/ No (N) with details; if no data indicate unknown (U)]
Part – 1 : Justification for import

Sl. No. Parameters Y/N/U Details Ref. Data
Code Quality
No. code
1. Purpose of the introduction
2. Whether the proposed objective can be met by
native species
IA
3. If not, kindly indicate;
a). Native species don’t have capability
b). Technology is not available for its culture
D
Part – 2 : Species Summery (taxonomic and general habitat)
Code Quality
1.
2.
3.
Scientific name
Common name(s)
Classification: Family Order
IN No. code
3.a) Taxonomic reference

A
4. Is the species difficult to distinguish from any
other species?
If yes, what species.
D
5 In the species to be introduced, a developed

strain of the original species or domesticated
farm stock. If it is a strain
a) Indicate strain development method
PE
(selection, hybridization, cross breeding

chromosome manipulation).
b) Give details of founder stocks and number
of generation from founder stock.
c) Can the species to be introduced produce all
male/ female/ sterile population?
(If so specify details).
M
6 Natural distribution [country of origin]

7 Climate of source region. (e.g. tropical,
subtropical, temperate, etc.)
8 The specie’s main ecosystem marine, estuarine,
warm freshwater, cold freshwater.
8.a) Other ecosystems found
9 Main habitat type –
River, lake/ reservoir, estuary, wetlands, reef,
inter tidal, mangroves
9.a) Other habitats
84
Part - 3 : Species assessment to judge if it is under prohibited category or for prohibited area
Code Quality
No. code
1. Is the species already present in India?
1.a) If yes, specify where available.
1.b) Can it meet present demand?
1.c) If no to 1.b, the reason.
2. If not available in India, what is the source
of the fish to be introduced?
Wild/ hatchery stock
Country and fish farm address
3. What is the population status of the species
IA
in its native range as classified by IUCN
or the threatened list of the country.
4. Would approval of this application further
endanger the species in its native range?
D
5. Does the species have potentially harmful
characteristics? If yes, provide details on
venomous spines, poisonous flesh/ toxins,
special defence mechanisms, etc.
6.
or pathogen of public health significance?
Specify the parasites or pathogens that have

been identified.
IN
Is the species known to be a host of any parasite
7. Do these pathogens already exist in India?

A
If yes, specify.
8. Would the present introduction have potential to
D
introduce disease harmful to human beings in

India?
9. Can the species play a role in the spread or
transmission of diseases or parasites that affect
PE
aquatic or terrestrial domestic, native or feral

animals?
Specify which parasite or disease.

10. Does the geographic area where introduction is
proposed come under prohibited area?
M
Part – 4: Ecological assessment

4.1
Code Quality
No. code
1. What are the main predators of the species
throughout the life cycle in its country of origin?
2. Do similar predators occur in India?
3. What are the ranges of the following physical
parameters in the natural habitat of the species?
Temperature - Upper / lower
85
pH - Upper / lower
Salinity - Upper / lower
Oxygen - Upper / lower
4. Does this combination or range of parameter
characteristics exist in the areas of India where
the organism is likely to be introduced?
Specify.
5. Does the species possess any accessory
breathing organs? If yes, specify.
6. Does the species possess any other
characteristics or behaviour that would enhance
its ability to survive drought, e.g. aestivation,
hibernation?If yes specify.
IA
7. Does the species have any unusual habitat
requirements? If yes, specify.
D
4.2
Code Quality
1.
2.
IN
Are the sexes of the species readily distinguishable?
If yes, at what size are they first distinguishable?
Male
Female
No. code
3. What is the size of maturity? Does the species

A
exhibit plasticity with this character. If so details.
4. What is the maximum length and weight the
species attains?
D
Male
Female
5. Has the species been bred in captivity?
PE
Specify condition/ situation-

pool/ pond
aquarium
others (specify)
6. Does the species breed readily in captivity
without any inducing agent?
M
Specify conditions-
7. What type of breeder is the species?
Live bearer, pelagic spawner, mouth brooder,
carrier of attached eggs, layer of adhesive eggs,
substrate spawner which guards the site,
substrate spawner which abandons the site,
parasitic (cuckoo) breeder.
Others (specify)
8. Does the species have particular requirements
for breeding sites? If so which of these:
Bedrock, boulders, gravel, sand, unknown, fine
sediments, aquatic plants (specify species),
timber-snags.
Others (specify)/ unknown
86
9. Does the habitat condition in the area where the

species is to be introduced meet the species
requirements for nest/breeding sites?
Specify.
10. Does the species utilize nest or shelter sites
similar to those used by Indian native species?
11. What breeding characteristics does the species
possess?
Obligate, opportunistic, serial (batch/ complete)
Other (Specify)
12. What is the fecundity of the species?
IA
13. What are the triggers for breeding?
Day length, feed availability, water level
(increase/ decrease), temperature rise/ fall,
first rainfall,. change in water conductivity,
social factors
D
Other (specify)/ unknown
14. Does the species exhibit
a) Schooling
b) Territoriality
4.3
Sl. No. Parameters
IN Y/N/U Details Ref. Data
Code Quality
No. code
A
1. Is the species known to hybridize?
a) In its’ natural habitat.
b) In aquaria/hatchery without inducing agent.
D
c) Only after inducement.

2. If the species has hybridized, what crosses are
known?
PE
3. Are the F1 progeny fertile?

4. With which Indian species there is a possibility
to hybridize and the reason.
4.4
M
Code Quality
No. code
1. How do you characterize the species with regard
to its feeding habit?
Carnivore/ piscivore, omnivore, herbivore,
detritus feeder
Specialist (scale, blood, slime, eggs,
larval stage, etc.) specify
2. Describe the range of food types of the species.
Does it show plasticity/ capacity to switch diet.
3. Name the Indian native species having similar
diet to the exotic species.
Give details.
87
4. With which Indian species there is possibility

of competition for food. Give reasons.
5. Is the species aggressive in its behaviour to
own kind?
Specify.
6. Is the species aggressive in its behaviour to
other species?
Specify.
7. Does the species cause any physical disturbance
to the aquatic environment such as disturbance
of the bottom, uprooting of flora, increasing
turbidity, etc.
8. If yes, what would be the expected nature and
IA
degree of such disturbance?
9. Is the species able to utilize/adapt to disturbed
habitats?
Specify.
D
4.5
Code Quality
1.
2.
IN
List all introductions (country, year, purpose)
Has the species established/ failed to establish
feral populations in areas where introduced?
No. code
Specify countries.
A
3. In the country (ies) it has established feral
population
a) What is its present distribution?
D
widespread/ limited?
b) Ecosystem and habitats feral population found
(see part: 6, 7)
PE
c) Rate of spreadrapid/ slow but steadily

expanding/ not expanding
4. What are the impacts (genetic, environmental,
economic and social in countries where feral
population have established.)
5. Please provide any other information you feel is
relevant to the report.
M
Part – 5 : Disease risk assessment

Code Quality
No. code
1. The parasites/ pathogens the exotic species is
known to harbour. List species name, taxonomic
position, country and year of report.
2. Which of the above cause disease and the
morbidity and mortality rate reported.
3. Which of the above is listed under OIE, NACA
or national list of diseases of concern
(Refer Annexure 13 & 14).
88
4. Which of the items under 3 are exotic to India or

so far not reported from India.
5. What is the mode of transmission of disease
vertical, horizontal or both.
6. Does each of the disease under 4 exhibit
difference in pathogenicity depending on
a) Life stage
b) Season
c) Particular culture conditions.
7. What is the documented spread of diseases
in other countries identified under item 4.
8. Which are the natural hosts of this pathogen.
9. Has experimental infection been proven.
IA
10. In which species the pathogen exists as
a) Latent infection
b) Vector
11. Are any of the hosts (other than candidate
D
species to be imported) listed in 8, 9, and 10
found in the country from where import is
proposed?
Data quality IN
Reviewer has to indicate data quality as per details given below.
A
Code Conditions
D
1. Definite documented evidence from particular species.

2. Definite documented evidence from species belonging to same genera.
3. Definite documented evidence from species belonging to same family.
PE
4. Studies to indicate that potential exists but are not conclusive.

5. In the absence of studies, based on overall assessment reviewer to indicate yes or no.
Reviewer’s Judgement:
M
❖ Can the species breed in Indian conditions? If yes, list such agro climatic zones/ coastal
regimes and what are the criteria for making judgement?
❖ Whether the candidate species can establish and spread?
❖ State the risk category based on risks?
❖ Under which secure system (high, medium, low secure or open system as defined in Guide-
lines) does the farm fall?
Reference
- All references to be listed separately and the code for reference to be indicated for each
item.
89
QUARANTINE
Though FAO/ NACA guidelines have extensively dealt with quarantine, some of the items
were not covered in detail. These have been identified and information available from Australian
Quarantine Inspection Service has been presented below with some modifications covering.
1) Standards for transporting aquatic animal to India.

2) Guidelines for quarantine facilities for live aquatic animal.
3) Quarantine procedures to be adopted on arrival
4) Quarantine procedures to be adopted on arrival into India and at the quarantine facility.
5) Approved disinfection procedure.
STANDARDS FOR TRANSPORTING AQUATIC ANIMALS TO INDIA
IA
1. All aquatic animals in the consignment must be packaged in leak-proof bags, each bag con-
taining only one species. The bag must be colourless and sufficiently transparent to enable
proper inspection and identification of the aquatic animals and must not contain any extrane-
D
ous matter, unapproved plant material, pests or unauthorized species. The use of outer bags
of opaque materials or half-black bags to provide a dark shipping environment is acceptable,
provided the contents can be inspected to the satisfaction of competent authority.
2. IN
The inclusion of inert material such as zeolite, activated carbon, shredded plastic or dried
terrestrial plants is permitted provided the contents of the bag can be properly inspected to
the satisfaction of competent authority and the material is disinfected or destroyed as directed
by competent authority.
A
3. Each bag must be of a size and weight which will allow inspection to the satisfaction of com-
petent authority.
D
4. Each bag must be placed within polystyrene boxes or cartons fitted with a plastic lining. Each
box or carton must be clearly identified as part of a shipment/ consignment and be individually
identified.
PE
5. The consignment must be accompanied by documents which include the identification num-
ber of each box or carton, and the scientific name and number of the contained aquatic ani-
mals. It is recommended that the common names of the aquatic animals also be included in
the papers.
M
6. The aquatic animals in each bag must be stocked at a density that will facilitate inspection and
hence must not be overcrowded. When packed for export, aquatic animals must be placed in
clean water. The use of pH indicator in the water is permissible, provided it does not interfere
with inspection.
7. Each consignment of aquatic animals must be accompanied by a health certificate issued by

the Competent Authority of the exporting country, signed by an official with appropriate knowl-
edge of the health of the animals and the export premises.
2 Guidelines for quarantine facilities for live aquatic animals
It is the responsibility of the person setting up quarantine facilities to be aware of and to

ensure compliance with the requirements of all State and local government regulatory organi-
zations.
90
The requirements for quarantine premises to hold live aquatic animals are:
Criteria for selecting location:
i) The location of the approved quarantine premises must be such that designated officers can
make arrangements for inspection to the satisfaction of competent authority.
ii) The quarantine premises should not be located in an area classified by the appropriate State
or local authority
(a) as a flood prone area,
(b) area adjacent to a aquatic farming/ hatchery operation.
IA
iii) The facility is not used for any purpose other than as a place for the performance of quaran-
tine. The facility may share a building with other areas which are used for purposes other than
any activity connected with live aquatic organisms like storage of feed but must not be used
as an access-way to other parts of the building.
D
iv) The quarantine facility must have a quarantine sign prominently displayed on the entrance to
the satisfaction of competent authority. The sign must be on pattern of the internationally
v)
accepted quarantine sign.
IN
Approval of quarantine premises will be for a period of 12 months after which approval will
have to be renewed. The premises must be inspected by competent authority prior to ap-
proval to ensure that standards stipulated are met. Approved quarantine premises may be
inspected at other times to determine whether standards are being maintained. In addition to
A
those requirements identified above, competent authority may stipulate other measures for
the effective running of approved quarantine premises. Approval may be cancelled or sus-
pended if the standards are not maintained.
D
Setting up Quarantine Facility:

PE
i. The quarantine facility shall be in a walled or fenced area. The facility must be away from
other aquaculture establishments and natural water bodies. The distance will be decided by
AQIU. The wall or fence must be a minimum of 2 meters in height. There shall be no water
used in common by quarantined aquatic animals and any other operation involving eggs or
aquatic animals.
M
ii. The quarantine facility must be fully enclosed and the walls and floor must be impervious and
sufficiently smooth to enable adequate cleaning and disinfection. Windows may be installed
in the facility, but must be either sealed or, if capable of opening, must be adequately screened
to prevent entry of insects. The door must have a self-closer to ensure that it remains shut
after entry.
iii. The facility must be secured with locked access and only authorized persons will be allowed
entry.
iv. The floor must be able to retain all spill water, and should have sufficient slope to allow good
drainage into an approved septic tank system as followed by Australian Quarantine Authority.
The drainage outlet should be supplied with a plug or other means of closing the outlet.
Overseas water (water brought with consignment) and other wastewater must be disinfected
91
prior to discharge, provided this does not provide direct entry to natural waterways.
v. Lighting in the facility must be of sufficient intensity to permit adequate inspection of all aquatic
animals.
vi. The quarantine facility should have a covered enclosed area that can be used as a laboratory
for preparing samples and undertaking microscopic examination. Sterilized containers and
reagents should be available to collect samples for despatch to other laboratories for further
examination, if required.
vii. Intake water should be disinfected/ sterilized. Spring, ground, artesian and well waters, which
have no flora or fauna in them prior to entry into quarantine facility, are best and require no
further treatment. If surface waters are used, there is a risk that native pathogens and pests
IA
may cause disease outbreaks in the unit causing consequent difficulties in deciding whether
the pathogen is native to the water supply or was imported with the introduction.
viii. The facility should have tanks, ponds, pools and other containers of appropriate size and
D
volume as appropriate. They must provide good visibility of contained aquatic organisms.
ix. The premises should have a designated refrigerator used solely for the storage of clinical
x.
IN
materials before sending the same to laboratory. It should be located within the quarantine
facility.
There should be footbaths at the entrance of the quarantine facility for the disinfection of the
footwear.
A
Disinfection facilities:
i. Facilities must be available for proper disinfection of

D
a. Water brought with consignment
b. Equipment that come into contact with water or aquatic animals under quarantine.
PE
ii. Provision must be made for staff and inspectors to wash their hands prior to leaving quaran-
tine facility
iii. There should be provision for disinfection of all effluents from the quarantine facility in an
approved manner.
M
iv. The quarantine facility must have a chlorination system to chlorinate all effluents. The chlori-
nation system except the stock chlorine solution tank shall be enclosed within a walled and
roofed building. The stock chlorine solution tank shall be separately enclosed to prevent solu-
tion leakage. The chlorination system shall include an alarm, which is activated when the
amount of residual chlorine falls below 5 ppm. At that stage, discharge of effluents from the
facility will not be permitted.
3. Quarantine procedures to be adopted on arrival
i. Each consignment of aquatic animals entering India (including packaging) will be examined
on entry by inspectors of the competent authority who will evaluate their health, check that all
documentation is in order, that only approved species are included and that no material of
quarantine concern is present. Any prohibited species or materials of must be re-exported,
92
destroyed or treated to the satisfaction of competent authority, all at the importer’s expense.
After inspection, all the animals will be ordered into quarantine at a place approved by the
competent authority as aquatic animals quarantine facility (AAQF). On arrival at the premises,
the aquatic animals will be transferred using a net to new water in the facility.
ii. All bags, polystyrene boxes and cartons used for importing exotic aquatic organisms must be
either incinerated or effectively disinfected by methods approved by competent authority prior
to disposal
iii. All water imported with the consignment must be disinfected to the standards of competent
authority prior to disposal.
4. Operation of Quarantine Facilities
IA
i) All aquatic animals must be kept in units i.e. tanks or other approved containers. Units must
be kept clean at all times. They must be free of gravel, sand, plants, soil or shell grit and only
sterilisable materials (e.g. polypropylene) may be contained in the unit. Tanks must be fitted
D
with covers (or approved equivalent) to prevent the aquatic animals from jumping out of the
tanks and to minimize splash contamination. Each unit must contain only a single species of
aquatic animals.
ii) IN
Where separate consignment of animals share a water recirculation system, the animals may
only be approved for release from quarantine when the last consignment to enter the system
has satisfactorily completed its quarantine requirements. All aquatic animals sharing the sys-
tem may be subject to quarantine risk management measures (e.g. destruction, treatment or
detention beyond the normal quarantine period) if any aquatic animals in the system is sus-
A
pected to carry disease agents or pests of quarantine concern. In deciding on the need for
measures to be applied to all aquatic animals sharing a recirculation system, competent au-
thority will take into account the presence of water sterilization systems (e.g. ozonation or
D
ultraviolet irradiation).
iii) The quality of water used in the quarantine unit should be monitored at regular intervals to
PE
ensure that any mortality in the quarantine population is not due to environmental conditions
but to disease agents.
iv) Only authorized persons should be allowed entry in the quarantine facility. Entrance to the
quarantine facility is restricted to the importer, his nominated employees and officials of the
competent authority, or other persons approved by the competent authority. Once a person
M
has entered a quarantine facility, such person will not on the same day enter any other place
where live aquatic organisms are kept.
v) Any unusual levels of mortality or unusual signs of disease/ pests (levels of mortality or illness
above that normally observed in imported aquatic animals) must be reported to the competent
authority immediately. If the quarantine unit suffers a disease outbreak that cannot be con-
trolled, the diseased stocks must be destroyed and disposed off after sterilization in an ap-
proved manner, but not before notification to the appropriate government authority. The quar-
antine unit or the particular module and associated items must be disinfected prior to its reuse
vi) The importer must ensure that no aquatic animals leave the quarantine facility under any
circumstances without the approval of the competent authority, excepting dead aquatic ani-
mals moved to a nearby refrigerator or freezer. Aquatic animals may not be released from
93
quarantine until completion of the following quarantine detention periods and fulfilment of all
the requirements to the satisfaction of the competent authority:
vii) Approval of requests for prophylactic or therapeutic treatments will be considered by the com-
petent authority, taking into account the need to ensure that exotic disease agents are not
inadvertently released from quarantine. Any treatment may result in the extension of quaran-
tine detention period or other measures as deemed necessary by the competent authority.
viii) Where the competent authority has reason to believe at the end of the quarantine detention
period that the aquatic animals still present an unacceptable risk of introduction of disease or
pest, they may be kept in quarantine detention for further investigation, observation, treat-
ment, testing or for any other purpose appropriate to the circumstances. If the risk cannot be
IA
effectively managed, destruction of the aquatic animals will be ordered. The costs associated
with these measures will be borne by the importer.
ix) Permission may be granted by competent authority for healthy aquatic animals to be held in
the quarantine facility after release from quarantine provided that all the requirements are met
D
during the time that the aquatic animals remain in the quarantine facility. On completion of
quarantine, aquatic animals are to be transferred by a suitable net into clean water prior to
removal from the facility.
Records:
i)
IN
A standard record sheet of the competent authority must be maintained for each unit. A tem-
plate of a unit record sheet may be obtained from competent authority. Unit record sheets
must be legible and available for inspection by the officials of the competent authority during
A
the quarantine period and for 12 months thereafter.
ii) A record should be maintained for all water treatments.

D
iii) All drug/ chemical treatment of aquatic animals must have approval of the competent author-
ity and be recorded on unit record sheets.
PE
Disinfection:
i) All nets and equipment shared between units must be disinfected in the quarantine facility by
a method approved by competent authority before being used for other consignments of aquatic
animals or prior to removal from the quarantine facility.
M
ii) All equipment must be disinfected prior to their removal from the quarantine facility. All mate-
rial for filtering water must be disinfected prior to removal of water from the quarantine facility
or disposed off by incineration.
iii) Staff and visitors must leave their street footwear outside the quarantine facility, and use
separate waterproof footwear within the facility. The footwear used in the quarantine facility
must remain inside the facility. Alternately, footwear may be removed from be quarantine
facility after being cleaned and disinfected to the standards of the competent authority.
iv) Staff and visitors who make contact with aquatic animals or water within the quarantine facility
must wash their hands with soap and water prior to exiting the facility.
v) All the effluents from the quarantine facility should be regarded as potentially infectious and
must be disinfected in an approved manner before releasing to the wastewater.
94
5. Disinfection procedures
Water sterilization and disinfection of equipment should be effective against the more resis-
tant aquatic animals disease agents or pests. Disinfect ion/ sterilization protocols should re-
duce pathogen titres to levels below that likely to cause infection when exposed to a suscep-
tible host. The following disinfection/ sterilization protocols provide an indication of the level of
disinfection and/ or sterilization required. Alternate methods which provide equal or greater
level of quarantine security may also be used. But it needs the advance approval of compe-
tent authority.
Sterilization of wastewater (including overseas water): Chlorine is very toxic. Hence hypo
chlorite powders and concentrated hypochlorite solutions should be kept in sealed containers
IA
in well-ventilated area outside the quarantine facility. This is to prevent volatilization of chlo-
rine gas into the air with risk to staff, and to reduce the possibility of chlorine dissolving in
aquarium water, with risk of toxicity to aquatic animals.
i. All the water to be treated must pass though a filter capable of removing suspended organic
D
material prior to hypochlorite treatment.
ii. The water to be treated must pass to a retention vessel where sufficient hypochlorite must be
IN
added to achieve a final concentration of 200 ppm. Sodium hypochlorite (bleach) should be
used at 1.6 milliliters of hypochlorite solution (12.5% available chlorine) per litre of water,
while calcium hypochlorite powder (65-70% available chlorine) should be used at 0.3 g of
powder per litre of water.
iii. Following addition of hypochlorite, wastewater must be agitated for a period not less than 10
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minutes to ensure thorough mixing of hypochlorite and retained for a period not less than 1
hour.
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iv. After the one-hour retention period, the chlorine in the wastewater may be neutralized by
adding sodium thiosulphate (hypo) at a rate of 1.25 g (2.5 ml of 50% sodium thiosulphate
solution) per litre of treated wastewater, then agitated for not less than 10 minutes before
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discharge.
Disinfection of equipment, hands, footwear and dead aquatic organisms:
i. Units and unit equipment to be disinfected must be thoroughly cleaned and treated with hy-
pochlorite solution at 200ppm concentration for 5 minutes or with an iodophore solution con-
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taining 0.5% available iodine for 5 minutes or by other approved disinfection methods.
ii. Hands of the personnel should be thoroughly washed with soap and water to remove any
contaminant material, prior to exiting the quarantine facility.
iii. If footwear is to be removed from the quarantine facility, it should be clean and the soles and
lower portion of the footwear must be disinfected by immersion of the exterior surface in an
approved disinfectant such as a 5% solution of Betadine.
iv. All dead aquatic animals or eggs can be kept in a solution of 10% formalin for a minimum of 5
days before disposing off . The ratio of dead fish or fish eggs volume to solution volume shall
not be less than 1:5.
95
SURVEILLANCE AND REPORTING
Surveillance means a systematic series of investigations of a given population of aquatic

animals to detect the occurrence of disease for control purposes, and which may involve testing
samples of the population. It helps in early detection of exotic pathogens, thereby preventing
spread of the same to the aquatic organisms in the country. Surveillance will assist in evolving
zoning concept for diseases of concern and also in contingency planning.
The existing disease reporting system has to be strengthened with clear understanding of
flow of information through different channels. To bring uniformity in the diagnosis report, aquatic
disease diagnosis laboratory form is enclosed.
For surveillance, the active participation of State Fisheries Departments is essential. Mea-
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sures need to be developed for linking State Fisheries Departments with Network of Diagnostic
Laboratories to strengthen surveillance and reporting.
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Disease Outbreak Report Form
Date reported:
Reported by:
Name:
Address:
/ /
IN Date problem first noticed: /
Phone/ Fax/Email:
/
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Location of problem:
Contact name: Phone/ Fax/Email:
Address:
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Tick one box only for each affected species:

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Species ++++ +++ + Appearance of

affected affected and
dead animals
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++++: Mass mortalities; +++: moderate numbers of deaths; + few deaths
Current situation (Tick one or more boxes): Suspected cause (s):

Problem now over
Problem is continuing
Problem occurring elsewhere
Signature: ________
Place: ________Date: ________
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Annexure 14
OIE LISTED DISEASES
DISEASES OF CRUSTACEANS
Crayfish plague (Aphanomyces astaci)
Infectious hypodermal and haematopoietic necrosis
Infectious myonecrosis
Taura syndrome
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White spot disease
White tail disease
Yellow head disease
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DISEASES OF FISH
Epizootic haematopoietic necrosis
Epizootic ulcerative syndrome IN
Gyrodactylosis (Gyrodactylus salaris)
Infectious haematopoietic necrosis
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Infectious salmon anaemia
Koi herpesvirus disease
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Red sea bream iridoviral disease

Spring viraemia of carp
Viral haemorrhagic septicaemia
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DISEASES OF MOLLUSCS
Infection with Bonamia exitiosa
Infection with Bonamia ostreae
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Infection with Marteilia refringens

Infection with Perkinsus marinus
Infection with Perkinsus olseni
Infection with Xenohaliotis californiensis
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ANNEXURE 15
NACA LIST OF DISEASES IN ASIA-PACIFIC
Diseases prevalent in some parts of the region

Finfish diseases : Epizootic heamatopoietic necrosis*
Infectious heamatopoietic necrosis*
Oncorhynchus masou virus disease*
Infectious pancreatic necrosis**
Viral encephalopathy and retinopathy**
Epizootic ulcerative syndrome (EUS)**
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Bacterial kidney disease**
Mollusc diseases: Bonamiosis (Bonamia sp., B. ostreae)*

Marteiliosis (Marteilia refringens, M. sydneyi)*
Microcytosis (Mikrocytos mackini, M. roughleyi)*
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Perkinsosis (Perkinsus marinus, P. olseni)*
Crustacean disease: Yellow head disease*
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Infectious hypodermal and heamatopoietic necrosis (IHHN)**
White spot disease*
Baculoviral midgut gland necrosis**
Gill associated virus (GAV)***
Spawner mortality syndrome (‘Midcrop mortality syndrome’)**
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Diseases presumed exotic to the region, but reportable to OIE
Finfish diseases: Spring viremia of carp*
Viral heamorrhagic septicaemia*
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Mollusc diseases: Haplosporidiosis (Haplosporidium costale, H. nelsoni)*

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Other diseases of importance: These include the following diseases so far presumed,
but not proven, to be exotic to this region:
Finfish diseases: Channel catfish virus disease**
Infectious salmon anaemia**
Piscirickettsiosis**
Gyrodactylosis (Gyrodactylus salaris)**
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Enteric septicaemia of catfish**
Mollusc diseases: Iridovirus (Oyster velar disease)

Crustacean disease: Nuclear polyhedrosis baculovirosis (Baculovirus penaei)**
Crayfish plague (Aphanomyces astaci)**
Taura syndrome*
Necrotising hepatopancreatitis***
● OIE notifiable diseases
** OIE other significant diseases
*** Diseases other than OIE-listed diseases.
98
ANNEXURE
Guidelines 16
for Green Certification of Freshwater Ornamental Fish
IMAGES OF SOME FRESHWATER ORNAMENTAL FISHES
SUGGESTED FOR GI
Puntius denisonii (Day) Puntius chalakkudiensis Menon, Rema Devi & Thobias
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Puntius exclamatio Pethiyagoda & Kottelat Puntius filamentosus (Valenciennes)
IN
A
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Puntius manipurensis Menon, Rema Devi & Vishwanath Puntius fasciatus (Jerdon)
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Puntius sahyadriensis Silas Puntius pookodensis Anna Mercy & Eapen Jacob
Puntius narayani (Hora) Puntius jerdoni (Day)
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Puntius tambraparnici Silas Puntius arulius (Jerdon)
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Puntius ophicephatus (Raj) Puntius melanostigma (Day)
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Puntius sarana subnasutus (Valenciennes) Labeo nigriscens Day

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Gonoproktopterus curmuca (Hamilton) Gonoproktopterus thomassi (Day)
Labeo potail (Sykes) Tor malabaricus (Jerdon)
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Ledidopygopsis typus Raj Garra stenorhynchus (Jerdon)
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Garra hughi Silas Garra surendranathanii Shaji, Arun & Easa
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Devario malabaricus (Jerdon)
IN Devario neilgherriensis (Day)
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Betadevario ramachandrani Pramod et al. Barilius bakeri Day

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Barilius canarensis (Jerdon) Salmostoma horai (Silas)
Laubuca fasciata (Silas) Laubuca dadyburjori (Menon)
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Osteobrama bakeri (Day) Osteochilichthys nashii (Day)
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Brachydanio jaintianensis Nibedita Sen Tetraodon cutcutia Hamilton
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Tetraodon travancoricus Hora & Nair Carinotetraodon imitator Britz & Kottelat
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Dario dario (Hamilton) Badis badis (Hamilton)

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Travancoria elongata Pethiyagoda & Kottelat Schistura semiarmatus (Day)
Travancoria jonesi Hora Mesoneemacheilus triangularis (Day)
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Schistura denisoni denisoni (Day) Schistura nilgiriensis (Menon)
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Botia striata Rao Acanthocobitis moreh (Sykes)
IN
Nemacheilus monilis Hora
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Mesonemacheilus guentheri (Day)
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Oreonectes evezardi (Day) Homaloptera montana Herre

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Longischistura striatus (Day) Indoreonectes keralensis (Rita & Nalbant)
Channa barca (Hamilton) Channa bleheri Vierke
103
Channa diplogramme (Day) Channa aurantimaculata Musikasinthorn
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Badis assamensis Ahl Chaca chaca (Hamilton)
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A
Horabagrus nigricollaris Pethiyagoda & Kottelat Horabagrus brachysoma (Guenther)
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Etroplus canarensis Day Etroplus maculatus (Bloch)
Pristolepis marginata Jerdon Pristolepis fasciata (Bleeker)
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Glyptothorax madraspatanam (Day) Glyptothorax anamalaiensis Silas
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Erethistes hara (Hamilton) Horaglanis alikunhii Subash Babu & Nayar
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Microphis cuncalus (Hamilton) Macrognathus guentheri (Day)
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Pseudosphromenus dayi (Kohler) Nandus andrewi Ng & Jaafar

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Source:
Anna Mercy, T.V., A. Gopalakrishnan, D. Kapoor and W.S. Lakra, 2007. Ornamental Fishes of the Western Ghats of India.
National Bureau of Fish Genetic Resources, Lucknow - 226 002, India, 235 p.
Beta Mahatvaraj. Email: [email protected]
Easa, P.S. and Shaji, C.P. 2003. Biodiversity documentation for Kerala - Part 8: Freshwater fishes, KFRI Handbook No.
17, Kerala Forest Research Institute (KFRI). Peechi 680 653, Kerala, India. 127 p.
Ponniah, A.G. and Gopalakrishnan, A. (Eds.) 2000. Endemic Fish Diversity of the Western Ghats. NBFGR - NATP Publi-
cation - 1, National Bureau of Fish Genetic Resources (NBFGR), Lucknow - 226 002, U.P., India. 347 p.
Rahul G. Kumar. Email: [email protected]
Ramachandran, A. (Ed.) 2002. Breeding, farming and management of ornamental fishes. School of Industrial Fisheries,
Cochin University of Science and Technology (CUSAT), Kochi 682 016, Kerala, India. 203 p.
Silas E.G., 1952. Notes on fishes of genus Glyptothorax Blyth from Peninsular India with description of a new species.
Journal of Bombay Natural History Society, 50:367-370.
Shaji, C.P., Easa, P.S. & Gopalakrishnan, A. (2001). Field Guide to the Freshwater Fishes of the Western Ghats, KFRI-
NATP-NBFGR publication, National Bureau of Fish Genetic Resources (NBFGR), Lucknow - 226 002 & Kerala Forest
Research Institute (KFRI), Peechi 680 653, Kerala, India. 109 p.
105
GLOSSARY
1. Acclimatization area- Area where the fish is acclimatized to the captive conditions in the hold-
ing facility. In this facility, the fish is held till it accepts the feed.
2. Accreditation- means a procedure by which an authoritative body gives formal recognition
that a body or person is competent to carry out specific tasks.
3. BMP: Best Management Practices.
4. CBD – Convention on Biodiveristy.
5. Certification – means the procedure by which a third party gives written assurance (certificate
of conformity) that a product, process or service conforms to requirements specified.
6. Certifier or certification organization- a third party independent organization that assesses, on
a commercial basis, other organizations or individuals for their compliance to the Green Cer-
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tification standards.
7. Chain of custody- the sequence of commercial operations or people responsible for the col-
lection, culture and trade in freshwater ornamental fishes. This begins with the collectors and
extends to the retailer – sale and to the end buyer. For the retailer to be able to offer green
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certified ornamental fishes, all components of the chain of custody handling the organisms
must be certified.
8. CIFA: Central Institute of Freshwater Aquaculture, Bhubaneswar, Odisha, India.
9.
10.
11.
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CITES: Convention on International Trade of Endangered Species.
CMFRI: Central Marine Fisheries Research Institute, Kochi, Kerala, India.
DAA- Dead After Arrival- ie, from acclimatization through the holding period until the organism
is packed for onward shipping.
12. Declared shipping time – the maximum period of time the organism can maintain optimal
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health in a closed container for shipping.
13. DOA – Dead on Arrival- upon receipt and opening of the shipping container at the beginning
of the acclimatization period.
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14. Documentation system- the collection of written policy statements, procedures, work instruc-
tions and records that make up formal objective evidence to show that an organization or
individual complies with the requirements of the Green Certification standards.
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15. Export facility: The area where the fishes are held and conditioned before dispatching for
export. It should have the prescribed facilities. Some times the secondary holding facility in
itself can be export facility if it satisfies all the requirements of an export facility.
16. ICAR: Indian Council of Agricultural Research.
17. INFOFISH: Intergovernmental Organisation for Marketing Information & Technical Advisory
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Service for Fishery Products in the Asia Pacific Region.

18. MPEDA: The Marine Products Export Development Authority, Kochi, Kerala, India.
19. NACA. Network of Aquaculture Centres in Asia.
20. NBFGR: National Bureau of Fish Genetic Resources, Lucknow, India.
21. OFI: Ornamental Fish International.
22. OIE: Office International des Epizooties (World Organisation for Animal Health), Paris, France.
23. Primary Holding facility: The area where the fish collected from the wild is initially held for a
short period of time with minimum infrastructure facilities.
Secondary holding facility: The area where the fishes from primary holding facility are held for
a longer period of time than the former.
24. UNCTAD: United Nations Conference on Trade and Development.
25. WWF: World Wildlife Fund.
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gripper
cmyk
Phone: +91-484-2311979 Fax: +91-484-2312812
ISBN 978-81-910104-0-4
ISBN 978-81-910104-0-4
Phone: +91-484-2311979 Fax: +91-484-2312812

cmyk
gripper
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IN
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Phone: +91-484-2311979 Fax: +91-484-2312812
ISBN 978-81-910104-0-4

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THE MARINE PRODUCTS EXPORT DEVELOPMENT AUTHORITY

THE MARINE PRODUCTS EXPORT DEVELOPMENT AUTHORITY

© 2011, The Marine Products Export Development Authority

5.4. Source of water --------------------------------------------------------------------------------------- 8

5.5.1. Fish ponds ------------------------------------------------------------------------------------- 9

5.5.5. Quarantine cum conditioning area ----------------------------------------------------- 10

16.8. Annexure 8 - Biopsy Techniques --------------------------------------------------------------- 34

16.10.Annexure 10 - List of items for Water quality analysis section -------------------------- 39

16.14.Annexure 11 C - Log Book Format - Secondary Holding Facility ---------------------- 47

/ comments on the draft.

2. Dr. A. Ramachandran, Professor, School of Industrial Fisheries, Cochin University of Science

3. Dr. A. Gopalakrishnan, Principal Scientist, NBFGR Unit, Kochi

Mr. K.R. Pushpangadhan, Ornamental fish Exporter, Kerala

6. Mr. Kripan Sarkar, Ornamental fish breeder, West Bengal

thority (MPEDA) in organizing an “International Workshop on Green Certification of Ornamental

implementation of the guidelines.

“Conservation and sustainable development of freshwater ornamental fish sector involving

The gist of the various topics can be summarized as follows:

2. Guidelines for Primary Holding Facility

3. Guidelines for Secondary Holding Facility

4. Guidelines for Exporting Facility

❖ This section addresses the significance of environmental concerns which have to be

7. Best Management Practices (BMP)

8. Green Seal Labelling (Eco Labelling)

9. Documentation & Maintenance of Data

tained at each section is given as annexures to this chapter.

10. Agencies for Accreditation and Verification

❖ Accreditation Agency and Verification Agency should be constituted by MPEDA which

14. Geographical Indication of species

The Indian scenario

The objectives of the workshop included

expanding & diversifying market through green certification

❖ Formulating guidelines for green certification of ornamental fish species.

b) Prepare a road map for implementing the system.

3. Dr. A. Ramachandran, Professor, School of Industrial Fisheries, Cochin University of Science

6. Mr. K.R.Pushpangadhan, Exporter, Travancore Aquapets, IV/342 A, Kollayil House, Kumbalam

PROTOCOLS AND PROCEDURES

1.6.3. Natural aquatic flora should not be destroyed during collection.

1.7. Handling and transfer of fish

2. PRIMARY HOLDING FACILITY (model lay out given in Annexure - 1)

3.6. Packaging and transport as per BMP

the general process control strategies.

5.1.3. Production area shall be at least 70% of total farm area

species from escaping to the natural water bodies.

should be constructed in modules for good management practice.

signs of any distress or pathology

with sufficient aeration.

continuous basis following an approved method of disinfection (Annexure 6).

5.5.6.10. Disposal should be in 10% formalin kept in a closed carboy (Annexure-6).

5.5.10. Water and air supply equipments

5.5.10.2. Motor & Pumps

5.5.11.1. A standby generator should be provided.

5.5.12.2. Have walled partitions to direct water in a zig-zag or up-down manner to

6.1. Farming should be planned and practiced in an environmentally responsible manner in

consumer. Hence Green certification is proposed as an independent certification and verifi-

Green Certification Logo

9. DOCUMENTATION & MAINTENANCE OF DATA –

10. AGENCY FOR ACCREDITATION