UV300 – Operating manual Tethys Instruments

Safety warning and liability

Chemicals used as reagent or as used as cleaning solution or used to prepare calibration

solutions might be toxic, corrosive or irritant. Refer to the material safety data sheets (MSDS) for
each chemical. Wear protection glass and gloves.

To prevent electric shock:

- Connect this instrument only at a properly grounded

power socket

- Unplug the power cord before any servicing,

wiring or any operation inside the instrument

- Keep the door locked by the key

The documentation and/or Product are provided on an “as is”

basis only and may contain deficiencies or inadequacies. The
Documentation and/or Product are provided without warranty of
any kind, express or implied.

The manufacturer or its suppliers shall, regardless of any legal

theory upon which the claim is based, not be liable for any
consequential, incidental, direct, indirect, punitive or other
damages whatsoever (including, without limitation, damages for
loss of business profits, business interruption, loss of business
information or data, or other pecuniary loss) arising out the use of
or inability to use the Documentation and/or Product, even if the
manufacturer or its suppliers has been advised of the possibility of
such damages.

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Contents

1 Quick start …………………..………………………………………………… 8

2 Installation …………………………………………….…….………………… 10

2.1 Fixing …………………………………………………………………… 10

2.2 Hydraulic connections …………………………….………………….. 11
2.3 Flow diagrams ……….………………….……………………………... 12
2.4 Electrical connections ……….………………….…………………….. 14

2.4.1 4-20 mA output ….……………………………………………… 15

2.4.2 Wi-Fi ….…….…….……………………………………………… 16
2.4.3 Ethernet …………..……………………………………………… 16
2.4.4 RS232 ….…….………………………………….……..………… 17
2.4.5 RS485 …………………………………………….……………… 18
2.4.6 pH probe .…….…….………………………………………….… 19
2.4.7 Conductivity probe ……………………………………………… 19
2.4.8 4-20 mA input …………………………………………………… 20
2.4.9 Relay module …………………………………………………… 20
2.4.10 Logical input …………………………………………………… 21
2.4.11 Mains ….…….…………………………………………..……… 22
2.4.12 USB ….…….………..…………………………………..……… 22

3 Operating …………………………………………….……...………………… 23

3.1 Screens overview ……………………………………………………… 23

3.2 Graph process screen ………………………………………………… 25
3.3 Values process screen ……..………………………………………… 28
3.4 Zero screen ……...................………………………………………… 30

3.5 General settings screens ...…………………………………….….… 32

3.5.1 Measuring mode .................................................................... 33

3.5.2 Relay functions ...................................................................... 35
3.5.3 Clock .................................................................................... 39
3.5.4 Password ............................................................................. 39
3.5.5 Language .............................................................................. 40
3.5.6 RS232 ................................................................................... 40
3.5.7 RS485 ................................................................................... 45
3.5.8 Sequential multiplexing ......................................................... 46
3.5.9 Zeroing ................................................................................. 47
3.5.10 Logical input ........................................................................ 49
3.5.11 USB ..................................................................................... 50
3.5.12 Level .................................................................................... 52

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3.6 Help screen ……….....………………………………………….….… 53

3.6.1 RS232 test ............................................................................. 53

3.6.2 RS485 test ............................................................................. 53
3.6.3 MODBUS test ........................................................................ 55
3.6.4 12V DC outputs test ............................................................... 56
3.6.5 Relay modules test ................................................................. 56
3.6.6 Software release .................................................................... 57
3.6.7 Channel definition .................................................................. 57
3.6.8 Positions (detectors, modules...) ............................................ 58
3.6.9 Memory management ............................................................ 59
3.6.10 4-20 mA test ......................................................................... 61
3.6.11 Temperature test .................................................................. 62
3.6.12 ADC test ............................................................................... 63

4 Parameters …….................................…………….……...………………… 64

4.1 Aluminium
4.1.1 Principle …………………………………………………………. 64
4.1.2 Settings ….………………………………………………………. 65
4.1.3 Test ………………………………………………………………. 70
4.1.4 Calibration …………………………………………………...…. 72
4.1.5 Maintenance and troubleshooting …..………………………. 74
4.1.6 Specifications ……...………………………..…………………. 76

4.2 Chlorine (total residual)

4.2.1 Principle …………………………………………………………. 77
4.2.2 Settings ….………………………………………………………. 78
4.2.3 Test ………………………………………………………………. 83
4.2.4 Calibration …………………………………………………...…. 85
4.2.5 Maintenance and troubleshooting …..………………………. 87
4.2.6 Specifications ……...………………………..…………………. 89

4.3 Chlorophyll A
4.3.1 Principle …………………………………………………………. 90
4.3.2 Settings ….………………………………………………………. 91
4.3.3 Test ………………………………………………………………. 94
4.3.4 Calibration …………………………………………….……...…. 96
4.3.5 Maintenance and troubleshooting …..…………….….………. 98
4.3.6 Specifications ……...………………………..……….…………. 99

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4.4 Color
4.4.1 Principle …………………………………………………………. 100
4.4.2 Settings ….………………………………………………………. 101
4.4.3 Test ………………………………………………………………. 104
4.4.4 Calibration …………………………………………….……...…. 106
4.4.5 Maintenance and troubleshooting …..…………….….………. 108
4.4.6 Specifications ……...………………………..……….…………. 109

4.5 Conductivity
4.5.1 Principle …………………………………………………………. 110
4.5.2 Settings ….………………………………………………………. 111
4.5.3 Test ………………………………………………………………. 114
4.5.4 Calibration …………………………………………….……...…. 115
4.5.5 Maintenance and troubleshooting …..…………….….………. 117
4.5.6 Specifications ……...………………………..……….…………. 118

4.6 Dissolved oxygen

4.6.1 Principle …………………………………………………………. 119
4.6.2 Settings ….………………………………………………………. 120
4.6.3 Test ………………………………………………………………. 122
4.6.4 Calibration …………………………………………….……...…. 123
4.6.5 Maintenance and troubleshooting …..…………….….………. 125
4.6.6 Specifications ……...………………………..……….…………. 125

4.7 Hydrocarbons (PAH)

4.7.1 Principle …………………………………………………………. 126
4.7.2 Settings ….………………………………………………………. 127
4.7.3 Test ………………………………………………………………. 131
4.7.4 Calibration …………………………………………….……...…. 133
4.7.5 Maintenance and troubleshooting …..…………….….………. 135
4.7.6 Specifications ……...………………………..……….…………. 136

4.8 Iron
4.8.1 Principle …………………………………………………………. 137
4.8.2 Settings ….………………………………………………………. 138
4.8.3 Test ………………………………………………………………. 143
4.8.4 Calibration …………………………………………….……...…. 145
4.8.5 Maintenance and troubleshooting …..…………….….………. 147
4.8.6 Specifications ……...………………………..……….…………. 149

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4.9 Nitrate (NO3)

4.9.1 Principle …………………………………………………………. 150
4.9.2 Settings ….………………………………………………………. 151
4.9.3 Test ………………………………………………………………. 154
4.9.4 Calibration …………………………………………….……...…. 156
4.9.5 Maintenance and troubleshooting …..…………….….………. 158
4.9.6 Specifications ……...………………………..……….…………. 160

4.10 Nitrite (NO2)

4.10.1 Principle …………………………………………………………. 161
4.10.2 Settings ….………………………………………………………. 162
4.10.3 Test ………………………………………………………………. 167
4.10.4 Calibration …………………………………………….……...…. 169
4.10.5 Maintenance and troubleshooting …..…………….….………. 171
4.10.6 Specifications ……...………………………..……….…………. 173

4.11 ORP
4.11.1 Principle …………………………………………………………. 174
4.11.2 Settings ….………………………………………………………. 175
4.11.3 Test ………………………………………………………………. 178
4.11.4 Calibration …………………………………………….……...…. 179
4.11.5 Maintenance and troubleshooting …..…………….….………. 181
4.11.6 Specifications ……...………………………..……….…………. 181

4.12 pH
4.12.1 Principle …………………………………………………………. 182
4.12.2 Settings ….………………………………………………………. 183
4.12.3 Test ………………………………………………………………. 186
4.12.4 Calibration …………………………………………………...…. 187
4.12.5 Maintenance and troubleshooting …..………………………. 189
4.12.6 Specifications ……...………………………..…………………. 189

4.13 Phosphate (PO4)

4.13.1 Principle …………………………………………………………. 190
4.13.2 Settings ….………………………………………………………. 191
4.13.3 Test ………………………………………………………………. 196
4.13.4 Calibration …………………………………………………...…. 198
4.13.5 Maintenance and troubleshooting …..………………………. 200
4.13.6 Specifications ……...………………………..…………………. 202

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4.14 Temperature
4.14.1 Principle …………………………………………………………. 203
4.14.2 Settings ….………………………………………………………. 204
4.14.3 Test ………………………………………………………………. 207
4.14.4 Calibration …………………………………………….……...…. 208
4.14.5 Maintenance and troubleshooting …..…………….….………. 210
4.14.6 Specifications ……...………………………..……….…………. 211

4.15 Turbidity by absorbance (external probe)

4.15.1 Principle …………………………………………………………. 212
4.15.2 Settings ….………………………………………………………. 213
4.15.3 Test …………………………………………………………….... 215
4.15.4 Calibration ……………………………………………….…...…. 216
4.15.5 Maintenance and troubleshooting …..…………….....………. 217
4.15.6 Specifications ……...………………………..…………….……. 218

4.16 Turbidity by nephelometry (internal)

4.16.1 Principle …………………………………………………………. 219
4.16.2 Settings ….………………………………………………………. 220
4.16.3 Test …………………………………………………………….... 223
4.16.4 Calibration ……………………………………………….…...…. 225
4.16.5 Maintenance and troubleshooting …..…………….....………. 227
4.16.6 Specifications ……...………………………..…………….……. 228

4.17 UV254
4.17.1 Principle …………………………………………………………. 229
4.17.2 Settings ….………………………………………………………. 230
4.17.3 Test …………………………………………………………….... 234
4.17.4 Calibration ……………………………………………….…...…. 236
4.17.5 Maintenance and troubleshooting …..…………….....………. 239
4.17.6 Specifications ……...………………………..…………….……. 240

5 General Maintenance and troubleshooting ................................ 241

6 General specifications ................................................................. 242

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1 Quick start

Step 1
Unpack the analyser, check that nothing has been damaged during the transportation and fix it
on a wall. The dimensions are given on chapter 2.

Step 2
Connect the sample inlet and outlet with plastic tubing, ID 6.4 mm (1/4”) OD 9.6 mm (3/8”).
Refer to chapter 2 for inlet and outlet location if necessary.
Assemble the fitting elements as below:

With the peristaltic sampling pump, the inlet pressure must be limited to 1 Bar (equiv.
to 1000 hPa or 15 psi)

Step 3
Depending on the options, refer to chapter 2 to connect the 4-20 mA output or the RS232 cable.
If a Wi-Fi link has been chosen as an option, check that the antenna is properly fixed on the
right side of the enclosure.

Step 4
Connect the power cable to a grounded power socket.

To avoid electric shock, the analyser must be connected to the earth via the power
socket.

Step 5
Check that the date and time on the bottom right of the screen are correct. If not, refer to
chapter 3 for setting date and time.

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Step 6
Refer to chapter 3 to configure the optional interface (4-20 mA or RS232 or Ethernet or Wi-Fi).

Step 7
The default mode is the stopped mode (no automatic measurements, no automatic cleaning).

Set the measuring mode (continuous, periodic or extern) depending of the application (refer to
chapter no 3).

The analyser is factory calibrated and is now ready to start the measurements.

For checking and recalibration, refer to chapter 4 for each individual parameter.

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2 Installation

2.1 Fixing

Make 4 holes in the panel or in the wall for 8 mm diameter screws according to the pattern
below:

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2.2 Hydraulic connections

Refer to the drawing and table below for the hydraulic connections.
Flow diagrams are given on the chapter 2.3.

3 1

1 Sample inlet
2 Sample outlet

3 Cleaning solution inlet (H2SO4 5% otherwise specified)

4 Reagent 1 inlet
5 Reagent 2 inlet

The sample inlet and outlet must be connected with plastic tubing, ID 6.4 mm (1/4”) OD 9.6 mm
(3/8”).

Reagents or cleaning solution must be connected with plastic tubing, ID 3.2 mm (1/8”) OD 6.4
mm (1/4”)

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The double-ring fittings must be assembled on the tubing as shown below:

2.3.1 Flow diagram for direct absorbance or fluorescence (UV254, NO3, Colour, turbidity,
PAH, chlorophyll A)

4 3
2

H2SO4 5%

1 Flow cell
2 3-way solenoid valve
3 Cleaning pump
4 Optional sampling pump

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2.3.2 Flow diagram for colorimetric method (Cl2, NO2, Al, Fe, etc...)

1 5

Reagent 1
6

Reagent 2
7
4
2

H2SO4 5%

1 Flow cell
2 3-way solenoid valve
3 Cleaning pump
4 Optional sampling pump
5 Mixing pump
6 Reagent 1 pump
7 Reagent 2 pump

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2.4 Electrical connections

All the cables must be installed using the glands as show below.

1 USB key connector

2 Glands for inputs/outputs
3 Gland for the power cable

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2.4.1 4-20 mA output (optional)

The module OUT4-20-400 delivers an active 4-20 mA signal for PLC, recorders or SCADA
system. Each OUT4-20-400 module is affected to one measuring channel.

Refer to chapter 3 to set up the 4-20 mA module position and scale values for each channel.

The maximum allowed charge is 500 ohm.

No voltage must be applied on the 4-20 mA output.

The output has a galvanic insulation by optocouplers up to 1 KV DC.

Screw terminal J2

Pin 1 4-20 mA output (+)

Pin 2 4-20 mA output (-)
Note: pin 1 on left side

Max load: 500 Ω

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2.4.2 Wi-Fi port (optional)

The Wi-Fi module follows the IEEE 802.11b standard and is internally connected to a SMA
connector on the right side of the analyser.

The 2.4 GHz antenna must be fixed on this SMA connector as represented below.

Refer to chapter 3 (communications) to enable the Wi-Fi communication and to set up the
network name (SSID) and the WEP key.

2.4.3 Ethernet port (optional)

The Ethernet module provide a RJ-45 jack that meets the ISO 8877 requirements for
10/100BASE-T. See the following figure and table for pin orientation and pin assignments.

Refer to chapter 3 (communications) to select the Ethernet port and to set up the IP address
and mask.

The RJ-45 cable must go through a PG16 gland.

RJ-45 pin assignment

Pin 1 TXD+ Transmit data +

Pin 2 TXD- Transmit data -
Pin 3 RXD+ Receive data +
Pin 4 EPWR+ Power from switch +
Pin 5 EPWR+ Power from switch +
Pin 6 RXD- Receive data +
Pin 7 EPWR- Power from switch -
Pin 8 EPWR- Power from switch -

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2.4.4 RS232 port

The RS232 port has a Sub-D 9 pins male connector and is located on the screen board
DSP400TFT, position J15.

Refer to chapter 3 (communications) to select the communication mode and the baud rate.
The format is always 8 bit, no parity, 1 bit stop.

To connect this port to a laptop (directly or through or a USB adaptor), use a null cable (pin 2 to
pin 3, pin 3 to pin 2, pin 5 to pin 5).

See the following table for pin assignments.

RS232 port – Sub-D9 pin assignement

Pin 1 DCD (unused)
Pin 2 RXD Receive data
Pin 3 TXD Transmit data
Pin 4 DTR (unused)
Pin 5 GND Ground
Pin 6 DSR (unused)
Pin 7 RTS (unused)
Pin 8 CTS (unused)
Pin 9 RI (unused)
Note 1: Pin 4 and 6 are connected together
Note 2: Pin 7 and 8 are connected together

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2.4.5 RS485 port

The RS485 port has a 5-pin screw terminal located on the screen board DSP400TFT, position
J38 as shown below:

This port is dedicated to RS485 probes (DO, TSS, pH).

See the following table for pin assignments.

Pin 5 may be used to connect the RS485 cable shielding.

RS485 port – Pin assignment

Pin 1 +12V Power supply for probe
Pin 2 D- Data B
Pin 3 D+ Data A
Pin 4 GND Ground
Pin 5 EARTH Earth for shielding
Note: pin 1 on left side

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2.4.6 pH module (optional)

The module MPH400 accepts the direct connection of a pH electrode on the screw terminal B1.

For automatic temperature compensation, a Pt100* or Pt1000* sensor of the electrode must be
connected on B2.

Note 1: if B2 stay unconnected, the temperature is preset at 25 °C.

Note 2: the maximal recommended length for the cable is 10 meters (30 feet).
(*) platinum resistive element of 100 ohm or 1000 ohm at 0° C

Screw terminal B1 Screw terminal B2

Pin 1 pH input (+) Pin 1 Pt100/100 input

Pin 2 pH input (-) Pin 2 Pt100/1000 input

Note: pin 1 on left side Note: pin 1 on left side

2.4.7 Conductivity module (optional)

The module MCOND400 accepts the direct connection of a conductivity electrode on the screw
terminal B1. Conductivity constant of K=0.01, K=0.1, K=1 and K=10 are accepted, refer to
chapter 4 to setup the right constant.

For automatic temperature compensation, a Pt100* or Pt1000* sensor of the electrode must be
connected on B2. Refer to chapter 4 to adjust the temperature coefficient.

Note 1: if B2 stay unconnected, the temperature is preset at 25 °C.

Note 2: the maximal recommended length for the cable is 10 meters (30 feet).
(*) platinum resistive element of 100 ohm or 1000 ohm at 0° C

Screw terminal B1 Screw terminal B2

Pin 1 Cond. input Pin 1 Pt100/1000 input

Pin 2 Cond. input Pin 2 Pt100/1000 input

Note: pin 1 on left side Note: pin 1 on left side

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2.4.8 4-20 mA input module (optional)

The module MIN4-20-400 accepts the connection of a 4-20 mA output from any probe or
external measuring system. The floating input is insulated by optocouplers.

A 15V DC output (insulated, max 20 mA) is available on the connector B2. This output must be
used for any probe with electrical contact to the water sample (example: chlorine probe).

Screw terminal B1 Screw terminal B2

Pin 1 4-20 mA Pin 1 +15V

input (+) Pin 2 0V (floating)
Pin 2 4-20 mA Note: pin 1 on left side
input (-)
Note: pin 1 on left side
Max current: 20 mA
Input impedance: 100 Ω

2.4.9 Relay module (optional)

The module MRELAY400 gives on B1 a normally closed (NC) and a normally open (NO) relay
contacts for different functions. Refer to chapter 3 to select the right function, threshold, etc...

To prevent electromagnetic interferences, it's highly recommended to switch only low

voltage loads (12 or 24 VAC or DC).

Screw terminal B1
Pin 1 NO contact
Pin 2 Common
Pin 3 NC contact
Note: pin 1 on left side

Contact rating:
5A @ 277VAC/100VDC resistive

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2.4.10 Logical input module (optional)

The module MIL accepts two logical signals from a PLC to control the measurement cycles.

The START input is used to start a measurement cycle for all the channels when a pulse (6 to
48V DC) is received. The minimum pulse width is 200 mS. The external measuring mode must
be selected.

The INHIBIT input is used to stop and inhibit any further automatic measurement (periodic mode
or continuous mode) as long as this input is maintained high (6 to 48 VDC).

Note: as these two input signals refer to different measuring mode, it has no sense to use both
of them on a same application.

Screw terminal B1 Screw terminal B2

Pin 1 Start input (+) Pin 1 Inhibit input (+)

Pin 2 Start input (-) Pin 2 Inhibit input (+)
Note: pin 1 on left side Note: pin 1 on left side

Level: 6 to 48 VDC Level: 6 to 48 VDC

Input impedance: 10 KΩ Input impedance: 10 KΩ

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2.4.11 Mains connection

To avoid electric shock, the analyser must be connected to the earth via the power
socket. Check that the power socked is earthed.

Disconnect the power cord or mains before any wiring, connection or servicing inside
the analyser.

If all the conditions above are satisfied, plug the power cord into a grounded power socket.

If the power cord needs to be changed or removed, the mains must be connected to the screen
board DSP400TFT on the screw terminal J1 as follows:

Line
Earth
Neutral

2.4.12 USB

A USB key (memory stick) can be connected on the USB connector type A represented on the
drawing of the section 2.4 for measurements download, configuration download or upload, or for
upgrading the analyser software. Refer to the Operating section for using the USB key.

Disconnect the USB key as soon as the operation if finished as USB connection is
not watertight and as it may block the analyser's normal operation.

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3 Operating
When powered on, the analyser displays the last process screen selected after a fast hardware
check.

Then the measurements start automatically and the process values and graphs are updated as
soon as necessary (except in stopped mode that is the default mode after delivery, see on the
general parameters screen to change the mode).

3.1 Screens overview

To change the screen, press on one of the following button as shown below.
No password is required by default but it's recommended the user create one, refer to the
general parameters screens.

Process
graph screen

Process values
screen

Zero screen

Calibration Parameters Settings

Check screen Help screen
screen screen screen

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3.3 Graph process screen

The value process screen is one of the two running screen of the analyser. It is display a few
seconds after power on and remains displayed all the time unless another screen has been
selected.

This screen displays the very last measurement and the recorded measurements graph for the
selected channel on the right.

3 6

9 7 8 10 7 11

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1 Label of the measuring channel

The label is automatically set when the channel is created. It can be modified in the channel
parameters screen.

2 Unit of the measuring channel

The unit is automatically set when the channel is created. It can be modified in the channel
parameters screen.

3 Temperature

Display the auxiliary temperature measurement of the channel. It concerns only the channels
with automatic temperature compensation.

4 Channel value

This value is the very last measurement done for each channel. The number of digit can be
modified in the channel parameters screen. By default, negative values resulting from normal
measuring fluctuations or from a wrong zero are displayed. They can be eliminated and replace
by zero by changing a setting on the channel parameters screen.

5 High alarm threshold line

This line correspond to the high alarm threshod. This threshold is also used to define the default
Y scale.

6 Cursor move

This two keys move the cursor line right or left. The date, time and value at the current
intersection between the graph and the cursor is displayed.

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7 Previous and next graph period

This two keys select previous or next displayed graph period.

8 Time scale

This two keys select larger or smaller time scale from the following: 30 days, 5 days, 24 hours, 1
hour, 10 minutes.

9 Date and time field

This field displays the current date and time. To change the date or the date format, refer to the
clock screen on the general settings.

10 Measurements schedule field

Depending on the measuring mode, this field displays information about the measurements
schedule.

If an inhibit signal is send to the logical input module MIL (for any mode except the stopped
mode), or if a cleaning cycle is engaged, this field displays “HELD”.

Otherwise display will be as follows:

a) For continuous mode, this field displays “Measuring...” followed by the label of the
channel being currently acquired

b) For periodic mode:

- Between measurement cycle, the time remaining for the next measuring cycle is
displayed, example: “Next measurement: 00:03:24” means that the next measuring
cycle will occur in 3 min and 24 sec.

- During sampling time, this field displays “Sampling...”

- During settling down time (if any), this field displays “Settling down...”

- During measurement, the currently measured parameter is displayed, for example:

“Measuring Cl2...”

c) For external mode:

- As long as no measuring cycle is engaged, this field displays “Waiting...”

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- If a measuring cycle command has been received from the logical input module MIL
or through MODBUS communication, a measuring cycle starts and the display is one
of the following:

- During sampling time, this field displays “Sampling...”

- During settling down time (if any), this field displays “Settling down...”

- During measurement, the currently measured parameter is displayed, for

example: “Measuring Cl2...”

d) For stopped mode, this field displays “Stopped”.

11 Cleaning schedule field

Depending on the cleaning method, this field displays information about the cleaning schedule.

a) Manual cleaning (automatic cleaning deactivated)

This field displays “No cleaning”.

b) Automatic cleaning (default mode)

- Between cleaning cycle, the time remaining for the next cleaning cycle is displayed,
example: “Next cleaning: 01:03:24” means that the next cleaning cycle will occur in 1
hours 3 min and 24 sec.

- During purging by acid, the time remaining is displayed, example: “Purging... 00:00:15”
means that purging will continue during 15 sec.

- During the waiting time, the time remaining is displayed, example: “Waiting... 00:00:05”
means that waiting time will continue during 5 sec.

- During zeroing (if any), This field displays “Zeroing...”

- During the recovery time, the time remaining is displayed, example: “Recovery...
00:00:20” means that recovery will continue during 20 sec.

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3.4 Values process screen

The value process screen is one of the two running screen of the analyser. It is display a few
seconds after power on and remains displayed all the time unless another screen has been
selected.

This screen displays the very last measurement for all the active channels.

5 6 7

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1 Label of the measuring channel

The label is automatically set when the channel is created. It can be modified in the channel
parameters screen.

2 Unit of the measuring channel

The unit is automatically set when the channel is created. It can be modified in the channel
parameters screen.

3 Temperature

Display the auxiliary temperature measurement of the channel. It concerns only the channels
with automatic temperature compensation.

4 Channel value

This value is the very last measurement done for each channel. The number of digit can be
modified in the channel parameters screen. By default, negative values resulting from normal
measuring fluctuations or from a wrong zero are displayed. They can be eliminated and replace
by zero by changing a setting on the channel parameters screen.

5 Date and time field

This field displays the current date and time. To change the date or the date format, refer to the
clock screen on the general settings.

6 Measurements schedule field

Please refer to the previous screen.

7 Cleaning schedule field

Please refer to the previous screen.

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3.4 Zero screen

The zero screen enables to start a cleaning/zeroing cycle at any time, additionally to the
automatic cleaning/zeroing cycle that occur normally once per day.

1 Date and time of the last cleaning/zeroing cycles

The date and time of the ten last zeroing cycle are displayed on this window, followed by the
new offset applied compared to the last zero. This value should normally stays close to zero.

2 Scan the last cleaning/zeroing cycle

The two button enable to scan up and down the last ten cleaning/zeroing cycles.

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3 Cleaning/zeroing current operation

This line describes the current operation of the engaged cleaning/zeroing cycle.
The typical cycle is:

- Purging time (60 sec)

- Waiting time (5 sec)
- Zeroing for all the concerned channels (5 to 10 seconds)
- Recovery time (30 sec)

4 Start a cleaning/zeroing cycle (or stop it if already engaged)

This button starts a cleaning/zeroing cycle. If a cycle is already engaged by error, it may be
stopped by pressing on this button.

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3.5 General settings screens

This screen allows to select different screens to check and change the general configuration of
the analyser:

- Measuring mode
- Relays functions
- Time and date update
- Password
- Language
- RS232 communication
- RS485 communication
- Multiplex mode (sequential only)
- Zeroing
- Logical input module
- USB screen copy
- Reagent and cleaning solution level monitoring

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3.5.1 Measuring mode

This screen enable to select the right measuring mode. Most of the applications uses the
periodic mode.

1 Measuring mode

Four modes can be selected:

- Continuous mode

The measurements are continuously updated at the highest possible rate. The display and the
4-20 mA output (if any) are updated every second. This mode apply only for internal turbidity
and electrode-based measurements (pH, conductivity, DO, 4-20 mA input, external turbidity).

- Periodic mode

The measurements are done at a specific period of time define in the “Measuring period” field.

The display and the 4-20 mA output (if any) are updated at the end of the measurement cycle.
The measurements are stored in the internal memory at each cycle.

If the measuring cycle time is higher than the defined period, a new measurement will start
immediately after the previous one.

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The measurement can be held at anytime if an high level voltage has been applied on the inhibit
input of the logical module MIL (if any).

Even in periodic mode, internal turbidity and electrode-based measurements stay in continuous
mode.

- External mode

The measurement cycle is started or by a pulse (>0.3 sec) send to the start input of the logical
module MIL, or by writing a value of 1 in the start measurement register by a MODBUS
command.

- Stopped mode (default mode after delivery)

All the automatic measurements or automatic cleaning cycles are stopped.

This mode can be use during maintenance operations or for doing manual measurements to not
be disturbed by automatic measurements.

2 Measuring period

For periodic mode only, the measuring cycle occurs with a period of time defined in this filed.

3 Sampling time for periodic mode

For periodic mode only, the sampling pump is running before every measuring cycle during a
time defined in this field.

4 Settling down time

In periodic mode only, it’s possible to add a settling down time defined in this filed after the
sampling and before the measurement to allow suspended solid to go down.

This mode concerns only a few specific applications, in all other cases, let the default value of 0.

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3.5.2 Relays

This screen allows to define the position of the optional relay modules.
Up to 6 relay modules can be installed.

1 Relays function selection

Pressing on this button enable to check or select the function of the specified realy.
Refer to the next screen for the functions.

2 Relays module position

This field allows to enter the relay module position among the 12 available sockets M1 to M12.
On this example screen, relay no 1 is on position M9 and relay no 2 on position M10.

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This following screen allows to define the function of each relay module selected on the
previous screen.

In duplicated multiplex mode, the n first relays are systematically affected to the multiplexing of
the n streams. No changes can be done.

1 Relays function selection

The relay functions are:

- High alarm (the linked channel is defined on the next screen)

Activated when the measuring channel overpass the high alarm threshold.
Boundaries apply for electrode-based measurements according to the schematic below.

- Low alarm (the linked channel is defined on the next screen)

Activated when the measuring channel is under the low alarm threshold. Boundaries apply for
electrode-based measurements according to the schematic below.

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1 High alarm value

2 High alarm value - boundary
3 Alarm relay activated
4 Alarm relay deactivated

- Fault

Any error detected during a measuring cycle activates this relay until cleared by a new
measurement

- Ready

Relay activated when the analyser is ready to measure, deactivated during measuring cycle or
cleaning cycle

- External sampling synchronisation

In periodic mode only, it’s possible to synchronize a second external sampling pump through a
relay if this function is activated. On time and off time as define in the next screen.
External turbidity and dissolved oxygen (if declared) are exceptionally measured when the on
time is finished and after a delay specified in the "waiting time" field (next screen).
The sampling chronogram is shown below:

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- Multiplex external solenoid valve command

When the multiplex mode (duplicated channel mode) has been selected, the concerned relays
are automatically selected for this function.

- Waste collect

This relay function enable to drive an external solenoid valve to collect the sample to prevent
pollution after that the reagent has been added and during a limited purge time. This function
concern only the colorimetric methods.

- Purging

This function concerns only NH4 and H2S measurement. It enable to drive a solenoid valve to
drain the stripping pot at the end of the measuring cycle.

- Periodic

This function is used to drive an external filter cleaning cycle every n measurements. n is define
on the next screen.

Screen to define external sampling parameters and filter cleaning parameters:

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3.5.3 Clock

The following screen enable to set to date and time.

Note: any engaged measuring or cleaning cycle will be aborted in case of time change.

3.5.4 Password

The following screen enable to enter a password to access to any parameter screen access.
The initial password is 0 (no password entering). The code 3333 can be used in case of
password lose.

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3.5.5 Language

The following screen enable to set the language.

Note that some technical terms are note translated.

3.5.6 RS232 settings

This screen allows setting or changing the communication settings for the serial ports RS232.

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1 Communication mode

Two protocols are available: MODBUS protocol and HTTP/HTML5 protocol.

1) MODBUS

The MODBUS communication mode allows reading the last measurements or the error codes
as well as starting a new measuring or cleaning cycle. The MODBUS protocol is a standard and
simple protocol implemented on most of the SCADA systems. If necessary, the full protocol can
be downloaded at www.modbus.org .

Basic frames using the function no 3 as shown below allows to read the measurements:

Request from the master unit:

Slave
number
3 Address of first byte NB of bytes CRC16

Answer from the analyser:

Slave ……
3 NB of read bytes 1st byte 2nd byte CRC16
number

A CRC16 is used to check the integrity of the frame.

The frames must be in binary mode (also named RTU mode), not in ASCII mode.

Addresses of the last measurements:

Decimal address Hexadecimal address Value for

68 44H
Channel 1
69 45H
70 46H
Channel 2
71 47H
72 48H
Channel 3
73 49H
………… ………… …………
98 62H
Channel 16
99 63H

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Addresses of the error code:

Decimal address Hexadecimal address Value for

52 34H Channel 1
53 35H Channel 2
54 36H Channel 3
………… ………… …………
67 43H Channel 16

The measurement values follows the floating point standard IEEE754, format single 32 bit (4
bytes), higher first, lower last. Invalid codes are 1 byte long.

Address for starting of a measurement cycle:

Decimal address Hexadecimal address Value to write

49 31H 1

A reading at this address returns 0 only when the measurement is finished.

Address for starting of a cleaning cycle:

Decimal address Hexadecimal address Value to write

50 32H 1

A reading at this address returns 0 only when the measurement is finished.

2) HTTP/HTML5

This protocol allows through a Wi-Fi or an Ethernet interface to drive the analyser through an
internet browser.

This mode of communication is compatible with:

- Internet Explorer under Windows 7

- Opera under Android
- Safari for i-Phone4

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Configuration of the Wi-Fi module Acksys WL-DONGLE

- Push the switch SW1 bellow the RS232 connector in direction of the "SW1" label

- Connect the module directly to a PC with an emulation terminal software using the RS232
cable

- Start the terminal emulation software at 2400 baud, 8 bit, no parity, 1 stop bit, no flux control

- Enter the following configuration:

Set net IP 192.168.2.30

Set net mask 255.255.255.0

Set net SSID Tethys

Set net wepkey 1 0123456789

Set net usekey 1

Set net mode infra

Set serial port 80

Set serial mode raw

Set serial baudrate 115200

Save

Reset

Each line must be acknowledged by an "OK" reply.

- Push back the switch SW1 bellow the RS232 connector

- Connect the Acksys WL-DONGLE to the RS232 port of the UV300 analyser

- Double check that an Wi-Fi antenna is connected on the WL-DONGLE, "Ant 2" side

Configuration of the Ethernet module Acksys Cometh

- Push the switch below the RS232 connector of the Ethernet board in the “Admin” position .
The orange LED must blink twice per second.

- Connect the module directly to a PC with an emulation terminal software using the RS232
cable

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- Start the terminal emulation software at 2400 baud, 8 bit, no parity, 1 stop bit, no flux control

- Enter the IP address and net mask by the commands:

Set net IP X.Y.Z.T (X,Y,Z,T from 0 to 255)

Set net mask A.B.C.D (A, B, C, D from 0 to 255)

Note: the IP address and net mask must be chosen by the network administrator

Save the configuration by entering “save”, then “reset” as shown below.

The image cannot be display ed. Your computer may not hav e enough memory to open the image, or the image may hav e been corrupted. Restart y our computer, and then open the file again. If the red x still appears, y ou may hav e to delete the image and then insert it again.

- Push back the switch below the RS232 connector of the Ethernet board. The orange LED
must stop to blink.

- Connect a cable on the RJ45 connector to the network. The green LED “Link Ether” must be
ON immediately.

2 Port A Baud rate

The baud rate must be fixed strictly in accordance with the system connected on the RS232 port
and generally at the higher speed compatible with the media capacity.

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3 MODBUS slave number

Each device on a MODBUS network has a specific number. This number can be entered in this
field. The default value is 1.

3.5.7 RS485 settings

This screen allows to configure the RS485 probes the first time they are used.
Only one probe must be connected during this configuration phase.

1 Probe initialisation

Press on this key to initialise the RS485 probe.

If the operation failed, disconnect the probe, reconnect it and press again on
"RESET" key because the probe take in account the new baud rate only on power
on.

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3.5.8 Sequential multiplexing mode

This screen allows to configure the sequential multiplexing mode.

2 3

1 Stream selection

The ON/OFF keys allow to select the proper number of stream for the sequential mode.
In this mode, the measurements are done sequentially on a same existing channel.
The stream number is stored in the measurements records table.

2 Circulation time

This field allows to fix the circulation time of each stream for a good renew, function of the
sample flow.

3 Inhibition module position

The measurement of any stream can be inhibited by a continuous level on the logical input
module MIL, if for example there is no sample temporary. The logical module position must be
entered in this field.

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3.5.9 Zeroing settings

This screen allows to check or change the zeroing settings.

1 Cleaning/zeroing period

This field contains the period of time between two cleaning cycles.
The recommended period is 24 hours.

2 Purging time

This field contains the pumping time of the cleaning solution.

The default value is 60 seconds. It is recommended to keep this default value unless authorized
by the manufacturer.

A too short cleaning time may cause a bad zeroing with the auto-zeroing function.
Keep the default value of 60 seconds for a good zeroing.

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3 Waiting time

This field contains the waiting time between the end of the cleaning solution pumping and the
zeroing to enable any bubbles produced by the cleaning pump to go up.
The default value is 5 seconds. It is recommended to keep this default value unless authorized
by the manufacturer.

4 Recovery time after cleaning

This field contains the recovery time before starting again the measurements on the sample
after a cleaning operation. The default value is 30 seconds. It is recommended to keep this
default value unless authorized by the manufacturer.

A too short recovery time may cause erratic measurements during the few seconds
that follow a cleaning cycle.
Keep the default value of 30 seconds for a good sample recovery.

5 Auto zero

If acid cleaning is available, an auto-zero can be performed automatically on the acid cleaning
solution if selected.

If the auto-zero is selected, the cleaning solution must be prepared with pure water
and analysis grade acid to get a correct zeroing.

6 Acid cleaning function

If activated, the acid cleaning will start automatically according to the defined period.

It may be remove only in case of very specific conditions like the absence of
cleaning solution.

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3.5.10 Logical module position settings

This screen allows to declare the MIL logical module position.

If no MIL module is used, the position must remains at 0.

1 MIL module position

This field enable to declare the MIL module position.

2 Start input test

This button show the actual state of the START input. ON means that a level above 6V DC is
applied on the input.

3 Inhibit input test

This button show the actual state of the INHIBIT input. ON means that a level above 6V DC is
applied on the input.

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3.5.11 USB functions

The USB key can be used for 4 different functions:

a. Recorded measurements and diagnostic file download (default function)

The last 5000 measurements are recorded in the cyclic internal memory and are transferred to
the USB key as a text file when plugged on the USB port. The file name is MXXXXYYYY.txt with
XXXX being the board serial number and YYYY an incremental number starting at 0001.

Each line of measurement starts by the date and time as shown in the example below:

;Cl2;TURB;pH;COND;TEMP;;;;;;;;;;;
;mg/l;NTU; ;uS;C;;;;;;;;;;

01/04/13 11:04:04; 0.55; 0.32; 7.45; 35.19; 18.46; 0.00; 0.00; 0.00; 0.00; 0.00; 0.00; 0.00; 0.00; 0.00; 0.00; 0.00
01/04/13 11:14:04; 0.54; 0.38; 7.47; 37.78; 18.51; 0.00; 0.00; 0.00; 0.00; 0.00; 0.00; 0.00; 0.00; 0.00; 0.00; 0.00
01/04/13 11:24:04; 0.53; 0.36; 7.46; 36.54; 18.66; 0.00; 0.00; 0.00; 0.00; 0.00; 0.00; 0.00; 0.00; 0.00; 0.00; 0.00

The channel measurements are separated by a semicolon and can be imported to Excel® as
show below:

A diagnostic file is also transferred at the same time to the USB key as a text file.
It contains the main configuration parameters and the most important signals that may be useful
for troubleshooting.

The file name is DXXXXYYYY.txt with XXXX being the board serial number and YYYY an
incremental number starting at 0001.

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b. Screen copy

The current screen is copied on the USB key as a bmp format file (Windows® compatible) if the
screen copy function has been activated before as shown on the screen below.
This function is automatically deactivated after one hour or in case of power off.

c. Configuration backup and restore

The complete configuration can be backup and restored as explained on section 3.6.9.

d. Software update

The internal software of the analyser can be updated from a USB key by following this
procedure:

- Backup the current configuration on an USB key (refer to section 3.6.9).

- Copy the new software send by e-mail as an attachment one a USB key
- Switch off the analyser
- Plug the USB key
- Select the right file with the arrow keys in case different software releases are on the USB key
- Press on START

Once the software update is finished (about one minute), you must reload the configuration
from the USB key (refer to section 3.6.9).

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3.5.12 Level settings

This screen enable to check the level of the cleaning solution and reagent, and to indicate that a
refill has been done.
3 4

1 Level

This field displays the estimated level based on the previous refill, the pump flow rate, the tank
capacity and the time of use.

2 Refill button

This button must be pressed at each refill. It reposition the level at 100%.

3 Pump flow

This field enable to adjust the pump flow.

4 Tank capacity

This field enable to set the tank capacity.

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3.6 Help screens

3.6.1 RS232 test

This screen allows to test the RS232 port. Each time this screen is called, it send the sequence
"QWERTY0123456789".
Any character received during that this screen is displayed is written after the label "Received:".
It recommended to use a terminal emulator communication software to test the RS232 port.

3.6.2 RS485 test

This screen allows to list all the RS485 probes declared on measuring channels.
The probe model is displayed and followed by "ok" if recognised, otherwise a red message "No
probe" is displayed if the probe is not connected or not recognised.

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To test the port itself, press on "TEST COM": each time this screen is called, it send the
sequence "QWERTY0123456789".
Any character received during that this screen is displayed is written after the label "Received:".
It recommended to use a terminal emulator communication software to test the RS485 port.

To determine the address and configuration of any probe connected on the RS485 port, press
on "SCAN". All the 254 possible addresses are tested to check all the connected probes. The
status of the probe is displayed: linked to an measuring channel or unlinked.

If unlinked, the address can be entered in channel parameter screen.

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3.6.3 Modbus test

This screen displays the diagnostic MODBUS counters defined by the MODBUS protocol.

The counters can be all reset to zero by pressing the key RESET.

Counter 1 displays the total number of valid frames received, whatever they are intended or not
for the analyser.

Counter 2 displays the number of frames received with a CRC error, whatever they are
intended or not for the analyser.

Counter 3 displays the number exception answers preceded. Exception answer is produced in
case of unrecognized command or wrong parameter on a received frame.

Counter 4 displays the number of valid frames received specifically intended for the analyser.

Counter 5 displays the number of valid broadcast frames received. Broadcast frame are not
usual.

Counter 6 is not in use (for compatibility only).

Counter 8 displays the number of characters received with a format error, whatever they are
intended or not for the analyser.

Counter 9 displays the number of frames proceeds for which an answer has been sent to the
host.

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3.6.4 +12V DC output test

This screen enable to check individually the 12V DC outputs that drives internal pumps and
solenoid valves.

To activate an output, press on the key OFF.

To deactivated it, press again on the key.

3.6.5 Relay modules test

This screen enable to check individually the relays modules. The relay module position is
indicated on the right column.

To activate a relay, press on the key OFF. To deactivated it, press again on the key.

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3.6.6 Software release and board number

This screen displays the software release and the board number.

3.6.7 Channel creation

This screen allow to declare a parameter. It is normally used only by the manufacturer.
Press any key to declare a parameter if the corresponding hardware is installed.

The key DELETE allows to suppress an existing channel.

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3.6.8 Channel positions

This screen displays the optical elements and/or module position.

On this example, the turbidity channel has his photo detector on position no 2 (from left on the 8
photo detector sockets J8 to J15) and his source on position no 2 (from left on the 6 source
sockets J16 to J21)

The next screen displays the 4-20 mA module position.

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3.6.9 Memory settings

This screen allows to manage the memory content.

1 Clear measurement

This button clear all the recorded measurements.

2 Store the factory settings

This button store in the internal EEPROM all the analyser configuration. This function is
normally used only by the manufacturer.

3 Restore the factory settings

This button restore form the internal EEPROM all the analyser configuration defined when the
analyser was delivered. This function can be used in case of doubts about the current
configuration.

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4 Save the configuration on the USB key

This button save in a .cfg file on the USB key the analyser configuration. This function is
recommended before doing a software update as the configuration is erased.

5 Load the configuration on the USB key

This button load from the USB key all the analyser configuration previously saved by the
function above. The operator must choose the right configuration file in case of several
configuration files stored in the USB key.

6 Full re-initialisation

This button erase all the configuration (channel, parameters, general settings...).
It may use exceptionally in case of change of board.
The configuration must be re-introduced manually or from a configuration file saved on the USB
key.

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3.6.10 4-20 mA test

1 Label of the channel in test

2 Current value on the 4-20 mA output

This value can be checked with an ammeter connected directly on the 4-20 mA output
(disconnect previously any cable on the output).

3 Calibration

This button enable to calibrate the 4-20 mA output if the value slightly differ from the theoretical
ones.
First enter the value read on an ammeter for 4-20 mA (example 3.96 mA), then enter the value
read for 20 mA (example 19.95 mA).
Then the 4-20mA output is recalibrated. It can be tested with the function below.

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4 Scan the 4-20mA output

This button allows to scan the 4-20 mA output mA by mA to check the output or the connected
system.

5 Value for 20 mA output

This field allows to enter the measured value corresponding to the full scale of 20 mA.

6 Value for 4 mA output

This field allows to enter the measured value corresponding to the bottom of the scale at 4 mA.
Generally, this value stays at 0.

3.6.11 Temperature

This screen gives the temperature reading from the internal temperature probe.
This temperature do not apply for the external probes (pH, conductivity,...).

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3.6.12 ADC test

This screen displays the internal ADC (Analogue Digital Converter) values for the 8 first
channels.

The field offset enable to introduce an offset in order that the values are centred to zero
(photodiodes disconnected). This offset has no influence on the measurements but improve the
touch screen position accuracy.

The FLASH key launches flashes on all the connected light sources during a few seconds for
testing purpose only.

The arrow enable to display the next 8 ADC channels.

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4 Parameters
The next section describes parameter by parameter the measuring principle, settings, testing,
calibration, troubleshooting and specifications.

To avoid interferences with the automatic measurements, it's recommended to put

first the analyser in stopped mode (refer to the previous section)
.

4.1 Aluminium

4.1.1 Principle

The measuring principle is based on the pyrocatechol violet colorimetric method. A small
quantity of reagent and buffer are injected on the sample. After a fixed reaction time, the
developed colour is measured by the light absorption according to the Beer-Lambert law:

[C ] = k ⋅ log I 0 
 I1 

With [C ] : Sample concentration

k: Absorption coefficient (molecule specific)
I0: Light intensity before the sample
I1 : Light intensity after of the sample

The light is generated by a red LED centred at 630 nm.

1 Tri-color LED 4 Reagent pump(s)

2 Flow cell 5 Reagent tank(s)
3 Photo detector

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4.1.2 Settings

The settings are display on the two following screens.

First screen:

1 Label

This field allows changing the channel label displayed with the measurement. The label size is
limited to 6 characters.

2 Unit

This field allows changing the channel unit displayed with the measurement. The unit size is
limited to 6 characters.

3 High alarm value

The high alarm level can be changed by pressing on this field. A default value is preset when
the channel is created. The high alarm level is primary used to fix the high limit represented by a
red line on the recorded measurement graph. The limit is used as the minimum graph scale.

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The secondary use is to drive a high alarm relay if the relay button has been activated and if a
relay module has been installed on the selected module socket. In this case, the relay will be
activated as soon as the measurement overpass the high alarm value and will stay activated
until the measurement goes down below this value after subtracting the boundary value.

Refer to installation section for the relay wiring. Both functions NO (Normally Open) and NC
(Normally Closed) are available.

4 Low alarm value

The low alarm level can be changed by pressing on this field. A default value is preset when the
channel is created. The low alarm level is primary used to fix the low limit represented by a
green line on the recorded measurement graph.

The secondary use is to drive a low alarm relay if the relay button has been activated and if a
relay module has been installed on the selected module socket. In this case, the relay will be
activated as soon as the measurement underpass the low alarm value and will stay activated
until the measurement goes down below this value after adding the boundary value.

Refer to installation section for the relay wiring. Both functions NO (Normally Open) and NC
(Normally Closed) are available.

5 Number of decimals

This field allows changing the number of decimal displayed for the measurement value to adjust
the display to significant decimals only.

Note: this choice does not affect the measurements stored in the internal memory or the
MODBUS registers.

6 Negative values displayed

Negatives values are normal for most of the measurements as they result from normal
fluctuations if the measurement is close to zero.

By default, the display of negative values is allowed as negative values may also result from a
wrong zeroing. Consequently, it may be useful to be aware of such problem when seeing
strongly negative values.

But as negative values have no theoretical meaning, it’s possible to replace them by zero by
selecting "NO" for this function.

Note that this function do not apply to the recorded measurements or to the MODBUS registers.

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Second screen:

1 Scale factor

This field allows changing the scale factor of the channel.

Precautions must be taken before changing this value.

This factor is normally determined in factory to transform the basic measurement to a scaled
value in the final unit. It is has no reason to be changed except if optical elements are replaced.

2 Averaging

This field displays the number of elemental acquisitions averaged to form one measurement.
The default value is 50.

3 Sampling time

This field displays the sampling time specifically for this measurement.
A minimum time of 10 sec is recommended to allow a good renew of the sample.
It might be longer depending on the length and height of tubing to take the sample.

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4 Reagent time

This field displays the reagent time injection. The default value is 1 sec.
Shorter time may produce instable measurements or limiting the measuring range.
Longer time are not necessary and generate a waste of reagent.

5 Mixing delay or reaction time

This field displays the colorimetric reaction time. The default value is 120 sec and this delay is in
accordance with the standard method.
Shorter times may reduce the measurement stability, especially for low temperatures.

6 Waste time

This field displays the purge time after the measurement. The default value is 5 sec.
The waste can be collected during this time if a relay module declared with the "waste" function
is installed.

7 Temperature coefficient

The colorimetric reaction might be slightly affected by the temperature. This coefficient is used
for the automatic temperature compensation.

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Third screen:

1 Compensation

This button allows to compensate the sample turbidity. It's mandatory for turbid sample,
otherwise the measuring value should be underestimated or even negative.

2 LED selection

This field display the LED chosen for the measurement. Never change it, unless the colorimetric
method is changed.

3 Minimum graph Y scale

This field fix the minimum Y scale of the reaction curve.

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4.1.3 Test

The check screen displays the last measurement performed, whatever manual or automatic,
with the reaction curve.

5 6 7 8 9 10

1 Reaction curve

This curve corresponds to the last reaction curve, or to the current reaction during a measuring
cycle. Intermediate measurements are performed every 10 sec. The final measurement is the
average of the last 5 intermediate measurements represented by the green line.

2 Final value

This value in black corresponds to the final value of the last measuring cycle. It is display in grey
during a measuring cycle and correspond to the intermediate measurements.

3 Reference signal

This value corresponds to the reference signal before the reagent injection.
Typical values are between 1000 and 3000. It may be lower in case of turbid sample.

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4 Current signal

This value correspond to the current signal level during the measuring cycle, or to the final
signal value when finished.

5 Start cycle button

This button allows to start a new measuring cycle.

6 Stop button

This button stop an engaged measuring cycle or a running pump.

7 Sampling pump button

This button run the sampling pump for 10 seconds, for example to purge the circuit or the check
the sampling pump.

8 Reagent pump button

This button run the reagent pump for 10 seconds, for example to fill the reagent circuit during
the first installation.

9 Signal test button

This button display the LED signal. It should normally be between 1000 and 3000 on pure
water. The screen is shown below.

10 Test button

This button is reserved for factory testing.

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Signal screen:

4.1.4 Calibration

The calibration screen below enable to recalibrate the measurement channel.

See after this screen description the recommendations for recalibration.

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1 Last calibration window

This window displays the 10 last calibrations done with the date, time and the new calibration
factor.

2 Scan last calibrations

These two buttons allow to scroll up and down the 10 last calibration records.

3 Calibration factor

This field allows changing manually the calibration factor of the channel.
This factor is normally changed automatically while doing a calibration procedure by pressing on
the “ADJUST” button.

4 Offset

This field shows the internal offset applied for zeroing.

It is updated at each zeroing and has normally not to be changed.

5 Calibration procedure

This button start a calibration procedure.

The last measured value is displayed and must be validated (or re-entered).
Then the standard value must be entered.
When finished, a new calibration factor is determined and recorded on the calibration history.

6 Linearization curve

As the Beer-Lambert law is not linear for high absorbance values, a linearization curve is
entered to compensate automatically this non-linearity. This button display the linearization
curve and enable to enter or check the linearization values.

The Y-axis corresponds to the rough measurements entered on the M1 to M10 fields while the
X-axis corresponds to the standard or final measurement, entered on the S1 to S10 fields.

The M1-M10 and S1-S10 values must be strictly increasing. It’s recommended to put 0.0 and
0.0 as starting values for S1 and M1. The final value is linearly extrapolated between these
points. Unused points at the end of the table must strictly remains at 0.0 both for S and M.

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Recommendations for recalibration

The analyser is factory calibrated. However, periodical checking is recommended and a

recalibration might be necessary after several months depending of the conditions of use.

To recalibrate the analyser, proceed as follow:

- Prepare a 0.1 g/l aluminium standard. For this weight 0.233 g of aluminum sulfate
octadecahydrate Al2(SO4)3.18H2O and dilute in 1litre of distilled water to obtain a mere solution
of 0.1g/l Al.

Then take 10 ml of this mere solution and complete to 1 litre of distilled water to get a 1 mg/l Al
standard.

- Do a manual measurement on this standard using the check screen (as describe above).

- Then go to the calibration screen and press on the “ADJUST” button. Validate the last
measured value and then enter the standard value on the keypad (1 mg/l).

The calibration is finished. A new calibration factor has been calculated and has been recorded
inside the calibration history displayed on the check screen.

This new calibration factor will be taken in account for all the further measurements.

4.1.5 Maintenance and troubleshooting

Maintenance

The maintenance is limited to refilling of the two reagent bottles and the 5% H2SO4 cleaning
solution.

Troubleshooting

Symptoms Origin

Negative value - Very high turbidity on the sample that is not compensated

Activate the polynomial compensation on the 3rd screen of

parameters.

Value is too low - No water in the flow cell (clogging, bad inlet connection, fault in the
peristaltic pump)

- No reagent or fault in the reagent injection (pump failure, tube

pinched or clogged)

- Bad calibration: check or redo calibration.

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Value is too high - Bad calibration: check or redo a calibration.

- Interferences in the sample

Unstable value - Deposit or dirty on the flow cell, check the light level on the check
screen with pure water

- Moving optical part (check manually after transportation).

- Bad calibration, check with a standard.

Measuring errors

Error Signification Origin / Remediation

no

1 No signal at all - Photo detector disconnected or faulty

2 The light level is too - Check that there is no bubble inside the flow cell, if yes check
high the fittings

- Failure on the photo-detector board or cable

3 The light level is too - Sample concentration over range, check again with pure
low water

- Sample turbidity too high, check again with pure water

- Deposit or dirty on the flow cell

- Failure on the LED board

4 Alarm for no reagent - Refill the reagent tank

- Check the level detector connection

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4.1.6 Specifications

Specifications for pyrocatechol Violet method

Calibrated range: 0 – 2 mg/l Al

Measuring range: 0 – 10 mg/l Al

Measuring time: 120 seconds

Accuracy: +/- 0.01 mg/l or +/- 5% whichever is greater

Interferences

Known interferences with iron, bismuth, copper and tin.

Possible interferences with other elements.

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4.2 Total residual (or free) chlorine

4.2.1 Principle

The measuring principle is based on the DPD colorimetric method (US-EPA 330.5). A small
quantity of reagent and buffer are injected on the sample. After a fixed reaction time, the
developed colour is measured by the light absorption according to the Beer-Lambert law:

[C ] = k ⋅ log I 0 
 I1 

With [C ] : Sample concentration

k: Absorption coefficient (molecule specific)
I0: Light intensity before the sample
I1 : Light intensity after of the sample

The light is generated by a green LED centred at 525 nm.

1 Tri-color LED 4 Reagent pump(s)

2 Flow cell 5 Reagent tank(s)
3 Photo detector

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4.2.2 Settings

The settings are display on the two following screens.

First screen:

1 Label

This field allows changing the channel label displayed with the measurement. The label size is
limited to 6 characters.

2 Unit

This field allows changing the channel unit displayed with the measurement. The unit size is
limited to 6 characters.

3 High alarm value

The high alarm level can be changed by pressing on this field. A default value is preset when
the channel is created. The high alarm level is primary used to fix the high limit represented by a
red line on the recorded measurement graph. The limit is used as the minimum graph scale.

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The secondary use is to drive a high alarm relay if the relay button has been activated and if a
relay module has been installed on the selected module socket. In this case, the relay will be
activated as soon as the measurement overpass the high alarm value and will stay activated
until the measurement goes down below this value after subtracting the boundary value.

Refer to installation section for the relay wiring. Both functions NO (Normally Open) and NC
(Normally Closed) are available.

4 Low alarm value

The low alarm level can be changed by pressing on this field. A default value is preset when the
channel is created. The low alarm level is primary used to fix the low limit represented by a
green line on the recorded measurement graph.

The secondary use is to drive a low alarm relay if the relay button has been activated and if a
relay module has been installed on the selected module socket. In this case, the relay will be
activated as soon as the measurement underpass the low alarm value and will stay activated
until the measurement goes down below this value after adding the boundary value.

Refer to installation section for the relay wiring. Both functions NO (Normally Open) and NC
(Normally Closed) are available.

5 Number of decimals

This field allows changing the number of decimal displayed for the measurement value to adjust
the display to significant decimals only.

Note: this choice does not affect the measurements stored in the internal memory or the
MODBUS registers.

6 Negative values displayed

Negatives values are normal for most of the measurements as they result from normal
fluctuations if the measurement is close to zero.

By default, the display of negative values is allowed as negative values may also result from a
wrong zeroing. Consequently, it may be useful to be aware of such problem when seeing
strongly negative values.

But as negative values have no theoretical meaning, it’s possible to replace them by zero by
selecting "NO" for this function.

Note that this function do not apply to the recorded measurements or to the MODBUS registers.

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Second screen:

1 Scale factor

This field allows changing the scale factor of the channel.

Precautions must be taken before changing this value.

This factor is normally determined in factory to transform the basic measurement to a scaled
value in the final unit. It is has no reason to be changed except if optical elements are replaced.

2 Averaging

This field displays the number of elemental acquisitions averaged to form one measurement.
The default value is 50.

3 Sampling time

This field displays the sampling time specifically for this measurement.
A minimum time of 10 sec is recommended to allow a good renew of the sample.
It might be longer depending on the length and height of tubing to take the sample.

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4 Reagent time

This field displays the reagent time injection. The default value is 1 sec.
Shorter time may produce instable measurements or limiting the measuring range.
Longer time are not necessary and generate a waste of reagent.

5 Mixing delay or reaction time

This field displays the colorimetric reaction time. The default value is 120 sec and this delay is in
accordance with the standard method.
Shorter times may reduce the measurement stability, especially for low temperatures.

6 Waste time

This field displays the purge time after the measurement. The default value is 5 sec.
The waste can be collected during this time if a relay module declared with the "waste" function
is installed.

7 Temperature coefficient

The colorimetric reaction might be slightly affected by the temperature. This coefficient is used
for the automatic temperature compensation.

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Third screen:

1 Compensation

This button allows to compensate the sample turbidity. It's mandatory for turbid sample,
otherwise the measuring value should be underestimated or even negative.

2 LED selection

This field display the LED chosen for the measurement. Never change it, unless the colorimetric
method is changed.

3 Minimum graph Y scale

This field fix the minimum Y scale of the reaction curve.

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4.2.3 Test

The check screen displays the last measurement performed, whatever manual or automatic,
with the reaction curve.

5 6 7 8 9 10

1 Reaction curve

This curve corresponds to the last reaction curve, or to the current reaction during a measuring
cycle. Intermediate measurements are performed every 10 sec. The final measurement is the
average of the last 5 intermediate measurements.

2 Final value

This value in black corresponds to the final value of the last measuring cycle. It is display in grey
during a measuring cycle and correspond to the intermediate measurements.

3 Reference signal

This value corresponds to the reference signal before the reagent injection.
Typical values are between 1000 and 3000. It may be lower in case of turbid sample.

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4 Current signal

This value correspond to the current signal level during the measuring cycle, or to the final
signal value when finished.

5 Start cycle button

This button allows to start a new measuring cycle.

6 Stop button

This button stop an engaged measuring cycle or a running pump.

7 Sampling pump button

This button run the sampling pump for 10 seconds, for example to purge the circuit or the check
the sampling pump.

8 Reagent pump button

This button run the reagent pump for 10 seconds, for example to fill the reagent circuit during
the first installation.

9 Signal test button

This button display the LED signal. It should normally be between 1000 and 3000 on pure
water. The screen is shown below.

10 Test button

This button is reserved for factory testing.

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Signal screen:

4.2.4 Calibration

The calibration screen below enable to recalibrate the measurement channel.

See after this screen description the recommendations for recalibration.

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1 Last calibration window

This window displays the 10 last calibrations done with the date, time and the new calibration
factor.

2 Scan last calibrations

These two buttons allow to scroll up and down the 10 last calibration records.

3 Calibration factor

This field allows changing manually the calibration factor of the channel.
This factor is normally changed automatically while doing a calibration procedure by pressing on
the “ADJUST” button.

4 Offset

This field shows the internal offset applied for zeroing.

It is updated at each zeroing and has normally not to be changed.

5 Calibration procedure

This button start a calibration procedure.

The last measured value is displayed and must be validated.
Then the standard value must be entered.
When finished, a new calibration factor is determined and recorded on the calibration history.

6 Linearization curve

As the Beer-Lambert law is not linear for high absorbance values, a linearization curve is
entered to compensate automatically this non-linearity. This button display the linearization
curve and enable to enter or check the linearization values.

The Y-axis corresponds to the rough measurements entered on the M1 to M10 fields while the
X-axis corresponds to the standard or final measurement, entered on the S1 to S10 fields.

The M1-M10 and S1-S10 values must be strictly increasing. It’s recommended to put 0.0 and
0.0 as starting values for S1 and M1. The final value is linearly extrapolated between these
points. Unused points at the end of the table must strictly remains at 0.0 both for S and M.

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Recommendations for recalibration

The analyser is factory calibrated. However, periodical checking is recommended and a

recalibration might be necessary after several months depending of the conditions of use.

To recalibrate the analyser, proceed as follow:

- Prepare a 1 mg/l free chlorine standard. For this weight 0.891 g of potassium permanganate
(KMnO4) and dilute in 1litre of distilled water to obtain a mere solution of 1g/l free Cl2.

Then take 1 ml of this mere solution and complete to 1 litre of distilled water.

- Do a manual measurement on this standard using the check screen (as describe above).

- Then go to the calibration screen and press on the “ADJUST” button. Validate the last
measured value and then enter the standard value on the keypad (1 mg/l).

The calibration is finished. A new calibration factor has been calculated and has been recorded
inside the calibration history displayed on the check screen.

This new calibration factor will be taken in account for all the further measurements.

4.2.5 Maintenance and troubleshooting

Maintenance

The maintenance is limited to refilling of the two reagent bottles and the 5% H2SO4 cleaning
solution.

Troubleshooting

Symptoms Origin

Negative value - Very high turbidity on the sample that is not compensated

Activate the polynomial compensation on the 3rd screen of

parameters.

Value is too low - No water in the flow cell (clogging, bad inlet connection, fault in the
peristaltic pump)

- No reagent or fault in the reagent injection (pump failure, tube

pinched or clogged)

- Bad calibration: check or redo calibration.

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Value is too high - Bad calibration: check or redo a calibration.

- Interferences in the sample

Unstable value - Deposit or dirty on the flow cell, check the light level on the check
screen with pure water

- Moving optical part (check manually after transportation).

- Bad calibration, check with a standard.

Measuring errors

Error Signification Origin / Remediation

no

1 No signal at all - Photo detector disconnected or faulty

2 The light level is too - Check that there is no bubble inside the flow cell, if yes check
high the fittings

- Failure on the photo-detector board

3 The light level is too - Sample concentration over range, check again with pure
low water

- Sample turbidity too high, check again with pure water

- Deposit or dirty on the flow cell

- Failure on the LED board

4 Alarm for no reagent - Refill the reagent tank

- Check the level detector connection

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4.2.6 Specifications

Specifications for DPD method method

Calibrated range: 0 – 5 mg/l total residual chlorine

Measuring range: 0 – 10 mg/l total residual chlorine

Measuring time: 120 seconds

Accuracy: +/- 0.01 mg/l or +/- 5% whichever is greater

Interferences

Any oxidizing agent.

These are usually present at insignificant concentrations
compared to the residual chlorine concentrations.

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4.3 Chlorophyll A

4.3.1 Principle

The measuring principle is based on the red light fluorescence that is a typical characteristic of
the chlorophyll A

The light is generated by a pulsed xenon lamp to reach an ultra long life time.
The excitation filter limits the bandwidth of the excitation light, while the emission filter selects
the specific fluorescent light.

The detection is done by a high sensitivity photomultiplier.

A second detector (optional) is used to compensate the eventual absorbance of the sample due
to turbidity.

1 Xenon UV lamp 4 Emission filter

2 Excitation filter 5 Photomultiplier
3 Flow cell 6 Photo detector

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4.3.2 Settings

The settings are display on the two following screens.

First screen:

1 Label

This field allows changing the channel label displayed with the measurement. The label size is
limited to 6 characters.

2 Unit

This field allows changing the channel unit displayed with the measurement. The unit size is
limited to 6 characters.

3 High alarm value

The high alarm level can be changed by pressing on this field. A default value is preset when
the channel is created. The high alarm level is primary used to fix the high limit represented by a
red line on the recorded measurement graph. The limit is used as the minimum graph scale.

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The secondary use is to drive a high alarm relay if the relay button has been activated and if a
relay module has been installed on the selected module socket. In this case, the relay will be
activated as soon as the measurement overpass the high alarm value and will stay activated
until the measurement goes down below this value after subtracting the boundary value.

Refer to installation section for the relay wiring. Both functions NO (Normally Open) and NC
(Normally Closed) are available.

4 Low alarm value

The low alarm level can be changed by pressing on this field. A default value is preset when the
channel is created. The low alarm level is primary used to fix the low limit represented by a
green line on the recorded measurement graph.

The secondary use is to drive a low alarm relay if the relay button has been activated and if a
relay module has been installed on the selected module socket. In this case, the relay will be
activated as soon as the measurement underpass the low alarm value and will stay activated
until the measurement goes down below this value after adding the boundary value.

Refer to installation section for the relay wiring. Both functions NO (Normally Open) and NC
(Normally Closed) are available.

5 Number of decimals

This field allows changing the number of decimal displayed for the measurement value to adjust
the display to significant decimals only.

Note: this choice does not affect the measurements stored in the internal memory or the
MODBUS registers.

6 Negative values displayed

Negatives values are normal for most of the measurements as they result from normal
fluctuations if the measurement is close to zero.

By default, the display of negative values is allowed as negative values may also result from a
wrong zeroing. Consequently, it may be useful to be aware of such problem when seeing
strongly negative values.

But as negative values have no theoretical meaning, it’s possible to replace them by zero by
selecting "NO" for this function.

Note that this function do not apply to the recorded measurements or to the MODBUS registers.

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Second screen:

1 Scale factor

This field allows changing the scale factor of the channel.

Precautions must be taken before changing this value.

This factor is normally determined in factory to transform the basic measurement to a scaled
value in the final unit. It is has no reason to be changed except if optical elements are replaced.

2 Averaging

This field displays the number of elemental acquisitions averaged to form one measurement.
The default value is 200.

3 Temperature coefficient

The fluorescence is slightly affected by the temperature. This coefficient is used for the
automatic temperature compensation.

4 Temperature adjustment

The value act as an offset on the temperature measurement to adjust the temperature. This
adjustment is not critical because the eventual error is taken in account during the calibration.

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4.3.3 Test

The check screen displays the measurement on the sample that is inside the flow cell. Press on
the play key to update the measurement.

2 3 4 5 6 7

1 Measured value

This value can be updated on pressing on the play key.

2 Sampling pump button

This button run the sampling pump for 10 seconds to renew the sample.

3 Reagent pump button

This button injects the cleaning solution. It can be used to check the zero.

4 Stop button

This button stop an engaged measuring cycle or a running pump.

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5 Signal test button

This button display the lamp signal. It should normally be below 300 on pure water. The screen
is shown below.

6 Zero button

This button is used for zeroing the measurement.

Put first pure water inside the flow cell before pressing this button.

7 Start measurement

This button allows to start a new measuring cycle.

Signal screen:

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4.3.4 Calibration

The calibration screen below enable to recalibrate the measurement channel.

See after this screen description the recommendations for recalibration.

1 Last calibration window

This window displays the 10 last calibrations done with the date, time and the new calibration
factor.

2 Scan last calibrations

These two buttons allow to scroll up and down the 10 last calibration records.

3 Calibration factor

This field allows changing manually the calibration factor of the channel.
This factor is normally changed automatically while doing a calibration procedure by pressing on
the “ADJUST” button.

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4 Offset

This field shows the internal offset applied for zeroing.

It is updated at each zeroing and has normally not to be changed.

5 Calibration procedure

This button start a calibration procedure.

The last measured value is displayed and must be validated.
Then the standard value must be entered.
When finished, a new calibration factor is determined and recorded on the calibration history.

6 Linearization curve

As the Beer-Lambert law is not linear for high absorbance values, a linearization curve is
entered to compensate automatically this non-linearity. This button display the linearization
curve and enable to enter or check the linearization values.

The Y-axis corresponds to the rough measurements entered on the M1 to M10 fields while the
X-axis corresponds to the standard or final measurement, entered on the S1 to S10 fields.

The M1-M10 and S1-S10 values must be strictly increasing. It’s recommended to put 0.0 and
0.0 as starting values for S1 and M1. The final value is linearly extrapolated between these
points. Unused points at the end of the table must strictly remains at 0.0 both for S and M.

Recommendations for recalibration

The analyser is factory calibrated. However, periodical checking is recommended and a

recalibration might be necessary after several months depending of the conditions of use.

To recalibrate the analyser, proceed as follow:

- Take a 1 mg lyophilised chlorophyll A standard

- Dilute it first in 10 ml of alcohol (70% or more), then complete to 1 litre of distilled water. This
standard solution has a short life time, use only the same day !

- Do a manual measurement on this standard using the check screen (as describe above).

- Go on the calibration screen and press on the “ADJUST” button of the check screen. Validate
the previous measurement and then enter the standard value on the keypad.

The calibration is finished. A new calibration factor has been calculated and has been recorded
inside the calibration history displayed on the check screen. This new calibration factor will be
taken in account for all the further measurements.

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4.3.5 Maintenance and troubleshooting

Maintenance

The maintenance is limited to refilling of the 5% H2SO4 cleaning solution.

Troubleshooting

Symptoms Origin

Negative value - Bad zero or auto-zero: the cleaning solution has been prepared with
polluted water or fault in the acid cleaning system (no flow, pump
failure)

Value is too low - No water in the flow cell (clogging, bad inlet connection, fault in the
peristaltic pump)

- Bad zero or auto-zero: cleaning solution has been prepared with

polluted water or fault in the acid cleaning system (no flow, pump
failure)

- Bad calibration: check or redo a calibration

Value is too high - Bad calibration: check or redo the calibration.

Unstable value - Deposit or dirty on the flow cell, check the light level on the check
screen

- Moving optical part (check manually after transportation).

- Bad calibration, check with a standard.

Measuring errors

Error Signification Origin / Remediation

no

3 The light level is too - Check that the analyser door is closed to avoid the
high on the photomultiplier to receive ambient light
photomultiplier
- Check that there is no bubble inside the flow cell, if yes check
the fittings

- Failure on the photo detector board or cable (repair or

replace)

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4 The light level is too - Check that the analyser door is closed to avoid the photo
high on the reference detector to receive ambient light
photo detector
- Check that there is no bubble inside the flow cell, if yes check
the fittings

- Failure on the photo detector board or cable (repair or

replace)

6 The light level is too - Very turbid sample, recheck with pure water
low on the reference
photo detector - Deposit or dirty on the flow cell

- Failure on the photo detector cable (repair or replace)

- Failure on the photo detector board (replace)

- Failure on the xenon lamp circuit if no flashes are visible

during the measurement, check the connection of the
XENON400 board on the DSP400TFT board (the orange neon
lamp on the XENON400 board must always be on, if not
replace the XENON400 board) and check the lamp connection
on the XENON400 board

4.3.6 Specifications

Specifications

Calibrated range: 0 - 100 µg/l ChlA

Measuring range: 0 - 200 µg/l ChlA

Measuring time: 5 seconds

Accuracy: +/- 1 µg/l ChlA or +/- 5% whichever is greater

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4.4 Colour

4.4.1 Principle

The measuring principle is based on the light absorption around 350 nm according to the Beer-
Lambert law:

[C ] = k ⋅ log I 0 
 I1 

With [C ] : Sample concentration

k: Absorption coefficient (molecule specific)
I0: Light intensity before the sample
I1 : Light intensity after of the sample

The UV light is generated by a pulsed xenon lamp to reach an ultra long life time.

The light detection is performed by two photodiode detectors through interferential filters; one
centred around 350 nm while the other is used as reference to compensate turbidity or deposit
inside the flow cell.

1 Xenon flash lamp

2 Sample flow cell
3 Beam splitter
4 Peak interferential filter
5 Peak photo detector
6 Reference interferential filter
7 Reference photo detector

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4.4.2 Settings

The settings are display on the two following screens.

First screen:

1 Label

This field allows changing the channel label displayed with the measurement. The label size is
limited to 6 characters.

2 Unit

This field allows changing the channel unit displayed with the measurement. The unit size is
limited to 6 characters.

3 High alarm value

The high alarm level can be changed by pressing on this field. A default value is preset when
the channel is created. The high alarm level is primary used to fix the high limit represented by a
red line on the recorded measurement graph. The limit is used as the minimum graph scale.

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The secondary use is to drive a high alarm relay if the relay button has been activated and if a
relay module has been installed on the selected module socket. In this case, the relay will be
activated as soon as the measurement overpass the high alarm value and will stay activated
until the measurement goes down below this value after subtracting the boundary value.

Refer to installation section for the relay wiring. Both functions NO (Normally Open) and NC
(Normally Closed) are available.

4 Low alarm value

The low alarm level can be changed by pressing on this field. A default value is preset when the
channel is created. The low alarm level is primary used to fix the low limit represented by a
green line on the recorded measurement graph.

The secondary use is to drive a low alarm relay if the relay button has been activated and if a
relay module has been installed on the selected module socket. In this case, the relay will be
activated as soon as the measurement underpass the low alarm value and will stay activated
until the measurement goes down below this value after adding the boundary value.

Refer to installation section for the relay wiring. Both functions NO (Normally Open) and NC
(Normally Closed) are available.

5 Number of decimals

This field allows changing the number of decimal displayed for the measurement value to adjust
the display to significant decimals only.

Note: this choice does not affect the measurements stored in the internal memory or the
MODBUS registers.

6 Negative values displayed

Negatives values are normal for most of the measurements as they result from normal
fluctuations if the measurement is close to zero.

By default, the display of negative values is allowed as negative values may also result from a
wrong zeroing. Consequently, it may be useful to be aware of such problem when seeing
strongly negative values.

But as negative values have no theoretical meaning, it’s possible to replace them by zero by
selecting "NO" for this function.

Note that this function do not apply to the recorded measurements or to the MODBUS registers.

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Second screen:

1 Scale factor

This field allows changing the scale factor of the channel.

Precautions must be taken before changing this value.

This factor is normally determined in factory to transform the basic measurement to a scaled
value in the final unit. It is has no reason to be changed except if optical elements are replaced.

2 Averaging

This field displays the number of elemental acquisitions averaged to form one measurement.
The default value is 200.

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4.4.3 Test

The check screen displays the measurement that responds directly to what is inside the flow
cell.

Press on the play key to update the measurement.

2 3 4 5 6 7

1 Measured value

This value is updated by pressing on the play button.

2 Sampling pump button

This button run the sampling pump for 10 seconds to renew the sample.

3 Reagent pump button

This button injects the cleaning solution. It can be used to check the zero.

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4 Stop button

This button stop an engaged measuring cycle or a running pump.

5 Signal test button

This button display the lamp signal. It should normally be between 1000 and 2500 on pure
water. The screen is shown below.

6 Zero button

This button is used for zeroing the measurement.

Put first pure water inside the flow cell before pressing this button.

7 Start measurement

This button updates the measurement directly with what is inside the flow cell.

Signal screen:

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4.4.4 Calibration

The calibration screen below enable to recalibrate the measurement channel.

See after this screen description the recommendations for recalibration.

1 Last calibration window

This window displays the 10 last calibrations done with the date, time and the new calibration
factor.

2 Scan last calibrations

These two buttons allow to scroll up and down the 10 last calibration records.

3 Calibration factor

This field allows changing manually the calibration factor of the channel.
This factor is normally changed automatically while doing a calibration procedure by pressing on
the “ADJUST” button.

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4 Offset

This field shows the internal offset applied for zeroing.

It is updated at each zeroing and has normally not to be changed.

5 Calibration procedure

This button start a calibration procedure.

The last measured value is displayed and must be validated.
Then the standard value must be entered.
When finished, a new calibration factor is determined and recorded on the calibration history.

6 Linearization curve

As the Beer-Lambert law is not linear for high absorbance values, a linearization curve is
entered to compensate automatically this non-linearity. This button display the linearization
curve and enable to enter or check the linearization values.

The Y-axis corresponds to the rough measurements entered on the M1 to M10 fields while the
X-axis corresponds to the standard or final measurement, entered on the S1 to S10 fields.

The M1-M10 and S1-S10 values must be strictly increasing. It’s recommended to put 0.0 and
0.0 as starting values for S1 and M1. The final value is linearly extrapolated between these
points. Unused points at the end of the table must strictly remains at 0.0 both for S and M.

Recommendations for recalibration

The analyser is factory calibrated. However, periodical checking is recommended and a

recalibration might be necessary after several months depending of the conditions of use.

To recalibrate the analyser, proceed as follow:

- Take a Pt-Co colour standard representative of the measuring range, but not lower than 100
Pt-Co

- Do a manual measurement on this standard using the check screen (as describe above).

- Go on the calibration screen and press on the “ADJUST” button of the check screen. Validate
the previous measurement and then enter the standard value on the keypad.

The calibration is finished. A new calibration factor has been calculated and has been recorded
inside the calibration history displayed on the check screen.

This new calibration factor will be taken in account for all the further measurements.

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4.4.5 Maintenance and troubleshooting

Maintenance

The maintenance is limited to refilling of the 5% H2SO4 cleaning solution.

Troubleshooting

Symptoms Origin

Negative value - Bad zero or auto-zero: the cleaning solution has been prepared with
polluted water or fault in the acid cleaning system (no flow, pump
failure)

Value is too low - No water in the flow cell (clogging, bad inlet connection, fault in the
peristaltic pump)

- Bad zero or auto-zero: cleaning solution has been prepared with

polluted water or fault in the acid cleaning system (no flow, pump
failure)

- Bad calibration: check or redo a calibration

Value is too high - Bad calibration: check or redo the calibration.

Unstable value - Deposit or dirty on the flow cell, check the light level on the check
screen

- Moving optical part (check manually after transportation).

- Bad calibration, check with a standard.

Measuring errors

Error Signification Origin / Remediation

no

1 No signal at all from - Peak photo detector PH1 disconnected or failure

the peak photo
detector PH1

2 No signal at all from - Reference photo detector PH2 disconnected or failure

the reference photo
detector PH2

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3 The light level is too - Check that there is no bubble inside the flow cell, if yes check
high on the peak the fittings
photo detector PH1
- Failure on the photo detector board or cable (repair or
replace)

4 The light level is too - Check that there is no bubble inside the flow cell, if yes check
high on the reference the fittings
photo detector PH2
- Failure on the photo detector board or cable (repair or
replace)

5 The light level is too - Over range or very high turbidity. Recheck on pure water
low on the peak
photo detector PH1 - Dirty on the flow cell windows. Check if the automatic
cleaning system is working properly

- Lamp failure or lamp supply board failure. Check if flashes

are visible during the measurement. Check the neon indicator
N1 on the XENON400 board.

6 The light level is too - Over range or very high turbidity. Recheck on pure water
low on the reference
photo detector PH2 - Dirty on the flow cell windows. Check if the automatic
cleaning system is working properly

- Lamp failure or lamp supply board failure. Check if flashes

are visible during the measurement. Check the neon indicator
N1 on the XENON400 board.

4.4.6 Specifications

Specifications

Calibrated range: 0 - 500 Pt-Co

Measuring range: 0 - 2000 Pt-Co

Measuring time: 5 seconds

Accuracy: +/- 2 Pt-Co or +/- 5% whichever is greater

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4.5 Conductivity

4.5.1 Principle

The conductivity is the reciprocal of resistance and is determined by the ratio current/voltage
according to Ohm’s law.

1 l amps
C= =
R l volt

The conductivity is measured in Siemens by two immersed electrodes of an area A separated

by a distance d. Since the measured conductivity depends on the electrode geometry, the
measured conductivity must be multiplied by the cell constant to obtain the specific conductivity
expressed in Siemens per centimeter (S/cm).

d
The cell constant is defined by: k=
A

The conductivity of a solution is proportional to its ion concentration, as long as there is no ionic
interaction.

Typical specific conductivity and cell constant:

Solution Specific conductivity (µS/cm)

Absolute pure water 0.055
Boiler water 1
Tap water 50
Ocean water 50,000

Application Typical range (µS/cm) Recommended Cell constant K

Ultra-pure water 0 to 2 0.01
Pure water, boiler 1 to 200 0.1
River, tap water 10 to 2,000 1
Sea water, effluents 1,000 to 200,000 10

As conductivity depends of the temperature, a temperature sensor is generally include in the

electrode (100 ohm or 1000 ohm Platinum resistor) and must be connected to the instrument for
automatic temperature compensation (ATC). The default coefficient is 2% per ºC but may be
modified according to the solution typical coefficient obtain experimentally.

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4.5.2 Settings

The settings are display on the two following screens.

First screen:

1 Label

This field allows changing the channel label displayed with the measurement. The label size is
limited to 6 characters.

2 Unit

This field allows changing the channel unit displayed with the measurement. The unit size is
limited to 6 characters.

3 High alarm value

The high alarm level can be changed by pressing on this field. A default value is preset when
the channel is created. The high alarm level is primary used to fix the high limit represented by a
red line on the recorded measurement graph. The limit is used as the minimum graph scale.

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The secondary use is to drive a high alarm relay if the relay button has been activated and if a
relay module has been installed on the selected module socket. In this case, the relay will be
activated as soon as the measurement overpass the high alarm value and will stay activated
until the measurement goes down below this value after subtracting the boundary value.

Refer to installation section for the relay wiring. Both functions NO (Normally Open) and NC
(Normally Closed) are available.

4 Low alarm value

The low alarm level can be changed by pressing on this field. A default value is preset when the
channel is created. The low alarm level is primary used to fix the low limit represented by a
green line on the recorded measurement graph.

The secondary use is to drive a low alarm relay if the relay button has been activated and if a
relay module has been installed on the selected module socket. In this case, the relay will be
activated as soon as the measurement underpass the low alarm value and will stay activated
until the measurement goes down below this value after adding the boundary value.

Refer to installation section for the relay wiring. Both functions NO (Normally Open) and NC
(Normally Closed) are available.

5 Number of decimals

This field allows changing the number of decimal displayed for the measurement value to adjust
the display to significant decimals only.

Note: this choice does not affect the measurements stored in the internal memory or the
MODBUS registers.

6 Boundaries

The purpose of boundary is avoiding too frequent changes of state of the alarm relays what can
harm process devices like pumps or electric-valves. The boundary value is subtracted from the
high level value before leaving the high alarm state and reciprocally added to the low alarm level
before leaving the low alarm state. More details including a schematic are provided in the relay
section.

7 Negative values displayed

Negatives values are normal for most of the measurements as they result from normal
fluctuations if the measurement is close to zero.

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By default, the display of negative values is allowed as negative values may also result from a
wrong zeroing. Consequently, it may be useful to be aware of such problem when seeing
strongly negative values.

But as negative values have no theoretical meaning, it’s possible to replace them by zero by
selecting "NO" for this function.

Note that this function do not apply to the recorded measurements or to the MODBUS registers.

Second screen:

1 Scale factor

This field allows changing the scale factor of the channel.

Precautions must be taken before changing this value.

This factor is normally determined in factory to transform the basic measurement to a scaled
value in the final unit. It is has no reason to be changed except if elements are replaced.

2 Temperature coefficient

The conductivity of a solution is affected by the temperature. This coefficient is used for the
temperature compensation. The default value is 2% per °C. It may be adjusted depending of the
solution.

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3 Temperature adjustment

The temperature of the sample given by the internal temperature probe can be adjusted by this
field if it differs from the real temperature read on a accurately calibrated thermometer.

This adjustment is not really important as a difference of temperature is taken in account during
the calibration. The adjustment is supposed to stay within +/- 4 °C.

4.5.3 Test
2

4 3

1 Read value

This value is continuously updated. It may be compared to a standard solution.

2 Probe temperature

This value is the sample temperature given by the probe. If the probe temperature sensor is not
connected, this value remains fixed at 25.0 °C.

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3 Zero button

This button is used for zeroing the measurement.

Put first pure water on the electrode and wait for stabilization (few minutes) before
pressing this button.

4 Signal test

This button is used to display the two analogue-digital converters of the conductivity module for
testing purpose only and is reserved to the manufacturer.

4.5.4 Calibration

The calibration screen below enable to recalibrate the measurement channel.

See after this screen description the recommendations for recalibration.

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1 Last calibration window

This window displays the 10 last calibrations done with the date, time and the new calibration
factor.

2 Scan last calibrations

These two buttons allow to scroll up and down the 10 last calibration records.

3 Calibration factor

This field allows changing manually the calibration factor of the channel.

This factor is normally changed automatically while doing a calibration procedure by pressing on
the “ADJUST” button.

4 Offset

This field shows the internal offset applied for zeroing.

It is updated at each zeroing and has normally not to be changed.

5 Calibration procedure

This button start a calibration procedure.

The last measured value is displayed and must be validated.
Then the standard value must be entered.
When finished, a new calibration factor is determined and recorded on the calibration history.

6 Linearization curve

For more accuracy, a linearization curve can be entered. This button display the linearization
graph and enable to enter or check the linearization values.

The Y-axis corresponds to the rough measurements entered on the M1 to M10 fields while the
X-axis corresponds to the standard or final measurement, entered on the S1 to S10 fields.

The M1-M10 and S1-S10 values must be strictly increasing. It’s highly recommended to put 0.0
and 0.0 as starting values for S1 and M1. The final value is linearly extrapolated between these
points. Unused points at the end of the table must strictly remains at 0.0 both for S and M.

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Recommendations for the calibration

The conductivity electrode must be checked on a regular base depending on the condition of
use, and recalibrated if necessary.

To recalibrate the conductivity electrode, proceed as follow:

1. Put the electrode in pure water

2. Do manual measurements using the check screen, wait for the stabilisation of the
conductivity value (a few minutes if the electrode is new or dry), then press on the ZERO key.

3. Then put the electrode in a standard solution for conductivity (example 210 µS for 100 mg/l
NaCl at 25°C)

4. Do manual measurement on this solution using the check screen, wait for the stabilisation of
the conductivity

5. Go on the calibration screen, press on the ADJUST key and enter the standard value on the
keypad.

The calibration is finished. A new offset and calibration factor has been calculated and has
been recorded on the calibration history displayed on the calibration screen.

These new offset and factor will be taken in account for all the further measurements.

4.5.5 Maintenance and troubleshooting

Maintenance

The conductivity probe must be cleaned on a regular base depending of the application (daily,
weekly or monthly).

Troubleshooting

Symptoms Origin

Negative value - Bad zero

Value is too low - No water in contact with the electrodes

- Dirty on the electrodes

- Bad calibration: check or redo a calibration

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Value is too high - Bad calibration: check or redo a calibration.

Unstable value - Electrical interference on the probe cable, put the probe cable away
from power cables

Measuring errors

Error Signification Origin / Remediation

no

1 Over range - Check on pure water

- Failure on the conductivity board (replace)

6.5.6 Conductivity specifications

Calibrated range: 0 – 1000 µS (for K=1 cell)

Measuring range: 0 – 1000 µS (for K=1 cell)

Measuring time: 2 seconds

Accuracy: +/1 µS or +/- 5%, whichever is greater

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4.6 Dissolved oxygen

4.6.1 Principle

The measuring principle is based on the fluorescence quenching by oxygen of a special

polymer membrane in contact with the sample. The light is emitted by a LED while two detectors
with red and blue filters are measuring the fluorescent light (red) and the reference light (blue).

In presence of oxygen, the fluorescence decreases that decreases the signal on the red
detector.

The probe has a RS485 interface under Modbus protocol and must be connected to the
dedicated RS485 port (J38) on the DSP400TFT board (for DSP400TFT board release E and
above).

1 LED light source 5 Red filter

2 Fluorescence photo detector 6 Luminophore
3 Reference photo detector 7 Insulation membrane
4 Blue filter

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4.6.2 Settings

The settings are display on the two following screens.

First screen:

1 Label

This field allows changing the channel label displayed with the measurement. The label size is
limited to 6 characters.

2 Unit

This field allows changing the channel unit displayed with the measurement. The unit size is
limited to 6 characters.

3 High alarm value

The high alarm level can be changed by pressing on this field. A default value is preset when
the channel is created. The high alarm level is primary used to fix the high limit represented by a
red line on the recorded measurement graph. The limit is used as the minimum graph scale.

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The secondary use is to drive a high alarm relay if the relay button has been activated and if a
relay module has been installed on the selected module socket. In this case, the relay will be
activated as soon as the measurement overpass the high alarm value and will stay activated
until the measurement goes down below this value after subtracting the boundary value.

Refer to installation section for the relay wiring. Both functions NO (Normally Open) and NC
(Normally Closed) are available.

4 Low alarm value

The low alarm level can be changed by pressing on this field. A default value is preset when the
channel is created. The low alarm level is primary used to fix the low limit represented by a
green line on the recorded measurement graph.

The secondary use is to drive a low alarm relay if the relay button has been activated and if a
relay module has been installed on the selected module socket. In this case, the relay will be
activated as soon as the measurement underpass the low alarm value and will stay activated
until the measurement goes down below this value after adding the boundary value.

Refer to installation section for the relay wiring. Both functions NO (Normally Open) and NC
(Normally Closed) are available.

5 Number of decimals

This field allows changing the number of decimal displayed for the measurement value to adjust
the display to significant decimals only.

Note: this choice does not affect the measurements stored in the internal memory or the
MODBUS registers.

6 Boundaries

The purpose of boundary is avoiding too frequent changes of state of the alarm relays what can
harm process devices like pumps or electric-valves. The boundary value is subtracted from the
high level value before leaving the high alarm state and reciprocally added to the low alarm level
before leaving the low alarm state. More details including a schematic are provided in the relay
section.

7 Modbus address

This field is the Modbus address of the probe that has been fixed inside the probe during the
initialisation sequence.
This value must not be changed, unless the RS485 scan screen indicates that the probe has not
the proper address.

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4.6.3 Test

1 Read value

This value is continuously updated.

2 Probe temperature

This value is the sample temperature given by the probe. It is used mainly for the calibration on
air.

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4.6.4 Calibration

The calibration screen below enable to recalibrate the measurement channel.

See after this screen description the recommendations for recalibration.

3 4 5

1 Last calibration window

This window displays the 10 last calibrations done with the date, time and the new calibration
factor.

2 Scan last calibrations

These two buttons allow to scroll up and down the 10 last calibration records.

3 Zero

This field allows to recalibrate the probe on zero solution. See after on the recommendations
how to prepare a zero solution.

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4 Calibration procedure

This button start a calibration procedure using a reference DO probe.

The last measured value is displayed and must be validated.
Then the reference value must be entered.
When finished, a new calibration factor is determined and recorded on the calibration history.

5 Calibration on air (100%)

The DO probe is factory calibrated but the full scale may be recalibrated if necessary (for
example once a year).

Before doing the calibration, allow the probe values to stabilize on air, both for the oxygen
concentration and the temperature by checking the values on the check screen.

Then, enter the atmospheric pressure read on a barometer in hPa.

If no barometer is available and if the installation is close to the sea level, validate the default
atmospheric value (1013 hPa) but a small error may result.

Recommendations for the calibration

The DO probe is factory calibrated and is normally stable for long period of time (one year).
It may be recalibrated if necessary.

Zero calibration

- Put the probe on zero-oxygen solution.

A zero-oxygen solution can be prepared by dissolving 10 g of sodium sulfite into 300 ml of pure
water and eventually adding a shake of cobalt chloride that accelerates the reaction. Allow a few
minutes of reaction time before using this solution.

Do not keep this solution more than a few hours.

- Do manual measurements using the check screen (as describe above), wait that the value
stabilizes (it may takes a few minutes). Then go to the calibration screen (flask icon) and press
on the ZERO key.

Full scale calibration on air

- Put the electrode on ambient air

- Do manual measurements using the check screen (as describe above), wait that both the
value and the temperature stabilizes (it may takes a few minutes).

- Then go to the calibration screen (flask icon) and press on the 100% key.

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- Enter the atmospheric pressure read on a portable barometer.

The probe is internally recalibrated. There is no change on the calibration factor.

4.6.5 Maintenance and troubleshooting

Maintenance

The DO probe must be cleaned on a regular base depending of the application (daily, weekly or
monthly). It may be cleaned automatically by sending pressurised air.

Troubleshooting

Symptoms Origin

Negative value - Bad zero

Value is too low - Dirty on the probe

- Bad calibration: check or redo a calibration

Value is too high - No water in contact with the probe

- Bad calibration: check or redo a calibration

Unstable value - badly immerged probe or high quantity of bubbles

Measuring errors

Error Signification Origin / Remediation

no

1 No connection - The probe is not connected or badly connected

- The probe is not properly configured (refer to the RS485

section for probe configuration).

A special initialisation must be done when the probe is

used for the first time

4.6.6 Dissolved oxygen specifications

Calibrated range: 0 – 25 mg/l O2

Measuring time: 60 seconds
Accuracy: +/- 0.02 mg/l O2 or +/- 5%, whichever is greater

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4.7 Hydrocarbons (PAH)

4.7.1 Principle

The measuring principle is based on the UV light fluorescence that is a typical characteristic of
the mono or poly aromatics hydrocarbons (PAH)

The UV light is generated by a pulsed xenon lamp to reach an ultra long life time.
The excitation filter limits the bandwidth of the excitation light, while the emission filter selects
the specific fluorescent light.

The detection is done by a high sensitivity photomultiplier.

A second detector (optional) is used to compensate the eventual absorbance of the sample due
to organic matter or suspended solids (turbidity).

1 Xenon UV lamp 4 Emission filter

2 Excitation filter 5 Photomultiplier
3 Flow cell 6 Photo detector

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4.7.2 Settings

The settings are display on the two following screens.

First screen:

1 Label

This field allows changing the channel label displayed with the measurement. The label size is
limited to 6 characters.

2 Unit

This field allows changing the channel unit displayed with the measurement. The unit size is
limited to 6 characters.

3 High alarm value

The high alarm level can be changed by pressing on this field. A default value is preset when
the channel is created. The high alarm level is primary used to fix the high limit represented by a
red line on the recorded measurement graph. The limit is used as the minimum graph scale.

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The secondary use is to drive a high alarm relay if the relay button has been activated and if a
relay module has been installed on the selected module socket. In this case, the relay will be
activated as soon as the measurement overpass the high alarm value and will stay activated
until the measurement goes down below this value after subtracting the boundary value.

Refer to installation section for the relay wiring. Both functions NO (Normally Open) and NC
(Normally Closed) are available.

4 Low alarm value

The low alarm level can be changed by pressing on this field. A default value is preset when the
channel is created. The low alarm level is primary used to fix the low limit represented by a
green line on the recorded measurement graph.

The secondary use is to drive a low alarm relay if the relay button has been activated and if a
relay module has been installed on the selected module socket. In this case, the relay will be
activated as soon as the measurement underpass the low alarm value and will stay activated
until the measurement goes down below this value after adding the boundary value.

Refer to installation section for the relay wiring. Both functions NO (Normally Open) and NC
(Normally Closed) are available.

5 Number of decimals

This field allows changing the number of decimal displayed for the measurement value to adjust
the display to significant decimals only.

Note: this choice does not affect the measurements stored in the internal memory or the
MODBUS registers.

6 Display mode

This button allows selecting the concentration display mode as PAH or as OIL.

If OIL is selected, the calibration factor is preset to a value of 10 to take in account a

predetermined ratio of 10% of aromatic hydrocarbons in the sample. This factor must be
adapted according to laboratory measurements made on the sample.

The measurements previously stored in the internal memory keep their original values.

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7 Negative values displayed

Negatives values are normal for most of the measurements as they result from normal
fluctuations if the measurement is close to zero.

By default, the display of negative values is allowed as negative values may also result from a
wrong zeroing. Consequently, it may be useful to be aware of such problem when seeing
strongly negative values.

But as negative values have no theoretical meaning, it’s possible to replace them by zero by
selecting "NO" for this function.

Note that this function do not apply to the recorded measurements or to the MODBUS registers.

Second screen:

1 Scale factor

This field allows changing the scale factor of the channel.

Precautions must be taken before changing this value.

This factor is normally determined in factory to transform the basic measurement to a scaled
value in the final unit. It is has no reason to be changed except if optical elements are replaced.

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2 Averaging

This field displays the number of elemental acquisitions averaged to form one measurement.
The default value is 200.

3 Temperature coefficient

The fluorescence is slightly affected by the temperature. This coefficient is used for the
automatic temperature compensation.

4 Temperature adjustment

The value act as an offset on the temperature measurement to adjust the temperature. This
adjustment is not critical because the eventual error is taken in account during the calibration.

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4.7.3 Test

The check screen displays the measurement that corresponds directly to what is inside the flow
cell.

Press on the play key to update the measurement.

2 3 4 5 6 7

1 Measured value

The measurement can be updated by pressing on the play button.

2 Sampling pump button

This button run the sampling pump for 10 seconds to renew the sample

3 Reagent pump button

This button injects the cleaning solution. It can be used to check the zero.

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4 Stop button

This button stop an engaged measuring cycle or a running pump.

5 Signal test button

This button display the lamp signal. The PM (photomultiplier) value should normally be below
300 on pure water, while the PH (reference photodiode) should be between 1000 and 2500. The
screen is shown below.

6 Zero button

This button is used for zeroing the measurement.

Put first pure water inside the flow cell before pressing this button.

7 Start measurement

This button update the measured value directly with what is inside the flow cell.

Signal screen:

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4.7.4 Calibration

The calibration screen below enable to recalibrate the measurement channel.

See after this screen description the recommendations for recalibration.

1 Last calibration window

This window displays the 10 last calibrations done with the date, time and the new calibration
factor.

2 Scan last calibrations

These two buttons allow to scroll up and down the 10 last calibration records.

3 Calibration factor

This field allows changing manually the calibration factor of the channel.
This factor is normally changed automatically while doing a calibration procedure by pressing on
the “ADJUST” button.

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4 Offset

This field shows the internal offset applied for zeroing.

It is updated at each zeroing and has normally not to be changed.

5 Calibration procedure

This button start a calibration procedure.

The last measured value is displayed and must be validated.
Then the standard value must be entered.
When finished, a new calibration factor is determined and recorded on the calibration history.

6 Linearization curve

As the Beer-Lambert law is not linear for high absorbance values, a linearization curve is
entered to compensate automatically this non-linearity. This button display the linearization
curve and enable to enter or check the linearization values.

The Y-axis corresponds to the rough measurements entered on the M1 to M10 fields while the
X-axis corresponds to the standard or final measurement, entered on the S1 to S10 fields.

The M1-M10 and S1-S10 values must be strictly increasing. It’s recommended to put 0.0 and
0.0 as starting values for S1 and M1. The final value is linearly extrapolated between these
points. Unused points at the end of the table must strictly remains at 0.0 both for S and M.

Recommendations for recalibration

The analyser is factory calibrated on phenol. Depending on the aromatic ratio of the sample, a
recalibration based on a laboratory measurement is normally necessary.

To recalibrate the analyser, proceed as follow:

- Collect about two litres of a significant sample in two different bottles

- Do a laboratory analysis on one of the two bottles.

- When the laboratory result is obtained, do a manual measurement on the second bottle using
the check screen describe above.

- Go on the calibration screen (flask icon) and press on the “ADJUST” button. Enter first the
measured value, then the value determined by the laboratory.

The calibration is finished. A new calibration factor has been calculated and has been recorded
inside the calibration history displayed on the check screen. This new calibration factor will be
taken in account for all the further measurements.

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4.7.5 Maintenance and troubleshooting

Maintenance

The maintenance is limited to refilling of the 5% H2SO4 cleaning solution.

Troubleshooting

Symptoms Origin

Negative value - Bad zero or auto-zero: the cleaning solution has been prepared with
polluted water or fault in the acid cleaning system (no flow, pump
failure)

Value is too low - No water in the flow cell (clogging, bad inlet connection, fault in the
peristaltic pump)

- Bad zero or auto-zero: cleaning solution has been prepared with

polluted water or fault in the acid cleaning system (no flow, pump
failure)

- Bad calibration: check or redo a calibration

Value is too high - Bad calibration: check or redo the calibration.

- Bubble on the sample

Unstable value - Deposit or dirty on the flow cell, check the light level on the check
screen

- Moving optical part (check manually after transportation).

- Bad calibration, check with a standard.

Measuring errors

Error Signification Origin / Remediation

no

3 The light level is too - Check that the analyser door is closed to avoid the
high on the photomultiplier to receive ambient light
photomultiplier
- Check that there is no bubble inside the flow cell, if yes check
the fittings

- Failure on the photo detector board or cable (repair or

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replace)

4 The light level is too - Check that the analyser door is closed to avoid the photo
high on the reference detector to receive ambient light
photo detector
- Check that there is no bubble inside the flow cell, if yes check
the fittings

- Failure on the photo detector board or cable (repair or

replace)

6 The light level is too - Very turbid sample, recheck with pure water
low on the reference
photo detector - Deposit or dirty on the flow cell

- Failure on the photo detector cable (repair or replace)

- Failure on the photo detector board (replace)

- Failure on the xenon lamp circuit if no flashes are visible

during the measurement, check the connection of the
XENON400 board on the DSP400TFT board (the orange neon
lamp on the XENON400 board must always be on, if not
replace the XENON400 board) and check the lamp connection
on the XENON400 board

4.7.6 Specifications

Specifications

Calibrated range: 0 - 10 mg/l phenol

(equivalent to 0 - 100 ppm oil for 10% of aromatics)

Measuring time: 5 seconds

Accuracy: +/- 0.01 mg/l phenol or +/- 5% whichever is greater

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4.8 Iron

4.8.1 Principle

The measuring principle is based on the phenanthroline colorimetric method. A small quantity of
reagent and buffer are injected on the sample. After a fixed reaction time, the developed colour
is measured by the light absorption according to the Beer-Lambert law:

[C ] = k ⋅ log I 0 
 I1 

With [C ] : Sample concentration

k: Absorption coefficient (molecule specific)
I0: Light intensity before the sample
I1 : Light intensity after of the sample

The light is generated by a green LED centred at 525 nm.

1 Tri-color LED 4 Reagent pump(s)

2 Flow cell 5 Reagent tank(s)
3 Photo detector

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4.8.2 Settings

The settings are display on the two following screens.

First screen:

1 Label

This field allows changing the channel label displayed with the measurement. The label size is
limited to 6 characters.

2 Unit

This field allows changing the channel unit displayed with the measurement. The unit size is
limited to 6 characters.

3 High alarm value

The high alarm level can be changed by pressing on this field. A default value is preset when
the channel is created. The high alarm level is primary used to fix the high limit represented by a
red line on the recorded measurement graph. The limit is used as the minimum graph scale.

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The secondary use is to drive a high alarm relay if the relay button has been activated and if a
relay module has been installed on the selected module socket. In this case, the relay will be
activated as soon as the measurement overpass the high alarm value and will stay activated
until the measurement goes down below this value after subtracting the boundary value.

Refer to installation section for the relay wiring. Both functions NO (Normally Open) and NC
(Normally Closed) are available.

4 Low alarm value

The low alarm level can be changed by pressing on this field. A default value is preset when the
channel is created. The low alarm level is primary used to fix the low limit represented by a
green line on the recorded measurement graph.

The secondary use is to drive a low alarm relay if the relay button has been activated and if a
relay module has been installed on the selected module socket. In this case, the relay will be
activated as soon as the measurement underpass the low alarm value and will stay activated
until the measurement goes down below this value after adding the boundary value.

Refer to installation section for the relay wiring. Both functions NO (Normally Open) and NC
(Normally Closed) are available.

5 Number of decimals

This field allows changing the number of decimal displayed for the measurement value to adjust
the display to significant decimals only.

Note: this choice does not affect the measurements stored in the internal memory or the
MODBUS registers.

6 Negative values displayed

Negatives values are normal for most of the measurements as they result from normal
fluctuations if the measurement is close to zero.

By default, the display of negative values is allowed as negative values may also result from a
wrong zeroing. Consequently, it may be useful to be aware of such problem when seeing
strongly negative values.

But as negative values have no theoretical meaning, it’s possible to replace them by zero by
selecting "NO" for this function.

Note that this function do not apply to the recorded measurements or to the MODBUS registers.

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Second screen:

1 Scale factor

This field allows changing the scale factor of the channel.

Precautions must be taken before changing this value.

This factor is normally determined in factory to transform the basic measurement to a scaled
value in the final unit. It is has no reason to be changed except if optical elements are replaced.

2 Averaging

This field displays the number of elemental acquisitions averaged to form one measurement.
The default value is 50.

3 Sampling time

This field displays the sampling time specifically for this measurement.
A minimum time of 10 sec is recommended to allow a good renew of the sample.
It might be longer depending on the length and height of tubing to take the sample.

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4 Reagent time

This field displays the reagent time injection. The default value is 1 sec.
Shorter time may produce instable measurements or limiting the measuring range.
Longer time are not necessary and generate a waste of reagent.

5 Mixing delay or reaction time

This field displays the colorimetric reaction time. The default value is 120 sec and this delay is in
accordance with the standard method.
Shorter times may reduce the measurement stability, especially for low temperatures.

6 Waste time

This field displays the purge time after the measurement. The default value is 5 sec.
The waste can be collected during this time if a relay module declared with the "waste" function
is installed.

7 Temperature coefficient

The colorimetric reaction might be slightly affected by the temperature. This coefficient is used
for the automatic temperature compensation.

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Third screen:

1 Compensation

This button allows to compensate the sample turbidity. It's mandatory for turbid sample,
otherwise the measuring value should be underestimated or even negative.

2 LED selection

This field display the LED chosen for the measurement. Never change it, unless the colorimetric
method is changed.

3 Minimum graph Y scale

This field fix the minimum Y scale of the reaction curve.

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4.2.3 Test

The check screen displays the last measurement performed, whatever manual or automatic,
with the reaction curve.

5 6 7 8 9 10

1 Reaction curve

This curve corresponds to the last reaction curve, or to the current reaction during a measuring
cycle. Intermediate measurements are performed every 10 sec. The final measurement is the
average of the last 5 intermediate measurements.

2 Final value

This value in black corresponds to the final value of the last measuring cycle. It is display in grey
during a measuring cycle and correspond to the intermediate measurements.

3 Reference signal

This value corresponds to the reference signal before the reagent injection.
Typical values are between 1000 and 3000. It may be lower in case of turbid sample.

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4 Current signal

This value correspond to the current signal level during the measuring cycle, or to the final
signal value when finished.

5 Start cycle button

This button allows to start a new measuring cycle.

6 Stop button

This button stop an engaged measuring cycle or a running pump.

7 Sampling pump button

This button run the sampling pump for 10 seconds, for example to purge the circuit or the check
the sampling pump.

8 Reagent pump button

This button run the reagent pump for 10 seconds, for example to fill the reagent circuit during
the first installation.

9 Signal test button

This button display the LED signal. It should normally be between 1000 and 3000 on pure
water. The screen is shown below.

10 Test button

This button is reserved for factory testing.

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Signal screen:

4.2.4 Calibration

The calibration screen below enable to recalibrate the measurement channel.

See after this screen description the recommendations for recalibration.

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1 Last calibration window

This window displays the 10 last calibrations done with the date, time and the new calibration
factor.

2 Scan last calibrations

These two buttons allow to scroll up and down the 10 last calibration records.

3 Calibration factor

This field allows changing manually the calibration factor of the channel.
This factor is normally changed automatically while doing a calibration procedure by pressing on
the “ADJUST” button.

4 Offset

This field shows the internal offset applied for zeroing.

It is updated at each zeroing and has normally not to be changed.

5 Calibration procedure

This button start a calibration procedure.

The last measured value is displayed and must be validated.
Then the standard value must be entered.
When finished, a new calibration factor is determined and recorded on the calibration history.

6 Linearization curve

As the Beer-Lambert law is not linear for high absorbance values, a linearization curve is
entered to compensate automatically this non-linearity. This button display the linearization
curve and enable to enter or check the linearization values.

The Y-axis corresponds to the rough measurements entered on the M1 to M10 fields while the
X-axis corresponds to the standard or final measurement, entered on the S1 to S10 fields.

The M1-M10 and S1-S10 values must be strictly increasing. It’s recommended to put 0.0 and
0.0 as starting values for S1 and M1. The final value is linearly extrapolated between these
points. Unused points at the end of the table must strictly remains at 0.0 both for S and M.

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Recommendations for recalibration

The analyser is factory calibrated. However, periodical checking is recommended and a

recalibration might be necessary after several months depending of the conditions of use.

To recalibrate the analyser, proceed as follow:

- Prepare a 0.2 mg/l Fe standard solution.

For this add slowly 20 ml of concentrated sulphuric acid (H2SO4) in 50 ml of distilled water.
Weight 1.404 g of iron sulphate hex hydrate (NH4)2Fe(SO4)2·6H2O and dilute in this solution.
Then complete to 1litre of distilled water to obtain a mere solution of 0.2 g/l Fe.

Then take 1 ml of this mere solution and complete to 1 litre of distilled water.

- Do a manual measurement on this standard using the check screen (as describe above).

- Then go to the calibration screen and press on the “ADJUST” button. Validate the last
measured value and then enter the standard value on the keypad (0.2 mg/l).

The calibration is finished. A new calibration factor has been calculated and has been recorded
inside the calibration history displayed on the check screen.

This new calibration factor will be taken in account for all the further measurements.

4.8.5 Maintenance and troubleshooting

Maintenance

The maintenance is limited to refilling of the two reagent bottles and the 5% H2SO4 cleaning
solution.

Troubleshooting

Symptoms Origin

Negative value - Very high turbidity on the sample that is not compensated

Activate the polynomial compensation on the 3rd screen of

parameters.

Value is too low - No water in the flow cell (clogging, bad inlet connection, fault in the
peristaltic pump)

- No reagent or fault in the reagent injection (pump failure, tube

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pinched or clogged)

- Bad calibration: check or redo calibration.

Value is too high - Bad calibration: check or redo a calibration.

- Interferences in the sample

Unstable value - Deposit or dirty on the flow cell, check the light level on the check
screen with pure water

- Moving optical part (check manually after transportation).

- Bad calibration, check with a standard.

Measuring errors

Error Signification Origin / Remediation

no

1 No signal at all - Photo detector disconnected or faulty

2 The light level is too - Check that there is no bubble inside the flow cell, if yes check
high the fittings

- Failure on the photo-detector board

3 The light level is too - Sample concentration over range, check again with pure
low water

- Sample turbidity too high, check again with pure water

- Deposit or dirty on the flow cell

- Failure on the LED board

4 Alarm for no reagent - Refill the reagent tank

- Check the level detector connection if any

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4.8.6 Specifications

Specifications for the Iron phenanthroline method

Calibrated range: 0 – 1 mg/l Fe

Measuring range: 0 – 2 mg/l Fe

Measuring time: 120 seconds

Accuracy: +/- 0.01 mg/l or +/- 5% whichever is greater

Interferences

Known as interfering: strong oxidizing agents, cyanide, nitrite, phosphates, chromium, zinc in
concentrations exceeding 10 times that of iron, cobalt and copper in excess of 5 mg/L, nickel
in excess of 2 mg/L.

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4.9 Nitrate

4.9.1 Principle

The measuring principle is based on the UV light absorption around 215 nm according to the
Beer-Lambert law:

[C ] = k ⋅ log I 0 
 I1 

With [C ] : Sample concentration

k: Absorption coefficient (molecule specific)
I0: Light intensity before the sample
I1 : Light intensity after of the sample

The UV light is generated by a pulsed xenon lamp to reach an ultra long life time.

The light detection is performed by two photodiode detectors through interferential filters; one
centred around 215 nm while the other is used as reference to compensate turbidity, deposit
inside the flow cell and organic matter.

1 Xenon flash lamp

2 Sample flow cell
3 Beam splitter
4 Peak interferential filter
5 Peak photo detector
6 Reference interferential filter
7 Reference photo detector

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4.9.2 Settings

The settings are display on the two following screens.

First screen:

1 Label

This field allows changing the channel label displayed with the measurement. The label size is
limited to 6 characters.

2 Unit

This field allows changing the channel unit displayed with the measurement. The unit size is
limited to 6 characters.

3 High alarm value

The high alarm level can be changed by pressing on this field. A default value is preset when
the channel is created. The high alarm level is primary used to fix the high limit represented by a
red line on the recorded measurement graph. The limit is used as the minimum graph scale.

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The secondary use is to drive a high alarm relay if the relay button has been activated and if a
relay module has been installed on the selected module socket. In this case, the relay will be
activated as soon as the measurement overpass the high alarm value and will stay activated
until the measurement goes down below this value after subtracting the boundary value.

Refer to installation section for the relay wiring. Both functions NO (Normally Open) and NC
(Normally Closed) are available.

4 Low alarm value

The low alarm level can be changed by pressing on this field. A default value is preset when the
channel is created. The low alarm level is primary used to fix the low limit represented by a
green line on the recorded measurement graph.

The secondary use is to drive a low alarm relay if the relay button has been activated and if a
relay module has been installed on the selected module socket. In this case, the relay will be
activated as soon as the measurement underpass the low alarm value and will stay activated
until the measurement goes down below this value after adding the boundary value.

Refer to installation section for the relay wiring. Both functions NO (Normally Open) and NC
(Normally Closed) are available.

5 Number of decimals

This field allows changing the number of decimal displayed for the measurement value to adjust
the display to significant decimals only.

Note: this choice does not affect the measurements stored in the internal memory or the
MODBUS registers.

6 Mode

This button allows changing the display mode: NO3 or N of NO3 according to the molar weight.

Note: this choice applies only to the further measurements.

7 Negative values displayed

Negatives values are normal for most of the measurements as they result from normal
fluctuations if the measurement is close to zero.

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By default, the display of negative values is allowed as negative values may also result from a
wrong zeroing. Consequently, it may be useful to be aware of such problem when seeing
strongly negative values.

But as negative values have no theoretical meaning, it’s possible to replace them by zero by
selecting "NO" for this function.

Note that this function do not apply to the recorded measurements or to the MODBUS registers.

Second screen:

1 Scale factor

This field allows changing the scale factor of the channel.

Precautions must be taken before changing this value.

This factor is normally determined in factory to transform the basic measurement to a scaled
value in the final unit. It is has no reason to be changed except if optical elements are replaced.

2 Averaging

This field displays the number of elemental acquisitions averaged to form one measurement.
The default value is 200.

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4.9.3 Test

The check screen displays the measurement that corresponds directly to what is inside the flow
cell.

Press on the play key to update the measurement.

2 3 4 5 6 7

1 Measured value

The measurement can be updated after pressing on the play button.

2 Sampling pump button

This button run the sampling pump for 10 seconds to renew the sample.

3 Cleaning pump button

This button injects the cleaning solution. It can be used to check the zero.

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4 Stop button

This button stop an engaged measuring cycle or a running pump.

5 Signal test button

This button display the lamp signal. The PH1 (peak photo detector) value should normally be
between 1000 and 2500 on pure water, PH2 (reference photodiode) should be between 1000
and 2500 also on pure water. PH1 decrease according to the nitrate concentration and the
turbidity of the sample. PH2 is affected only by the turbidity of the sample. The screen is shown
below.

6 Zero button

This button is used for zeroing the measurement.

Put first pure water inside the flow cell before pressing this button.

7 Start measurement

This button update the measured value directly with what is inside the flow cell.

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Signal screen:

4.9.4 Calibration

The calibration screen below enable to recalibrate the measurement channel.

See after this screen description the recommendations for recalibration.

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1 Last calibration window

This window displays the 10 last calibrations done with the date, time and the new calibration
factor.

2 Scan last calibrations

These two buttons allow to scroll up and down the 10 last calibration records.

3 Calibration factor

This field allows changing manually the calibration factor of the channel.
This factor is normally changed automatically while doing a calibration procedure by pressing on
the “ADJUST” button.

4 Offset

This field shows the internal offset applied for zeroing.

It is updated at each zeroing and has normally not to be changed.

5 Calibration procedure

This button start a calibration procedure.

The last measured value is displayed and must be validated.
Then the standard value must be entered.
When finished, a new calibration factor is determined and recorded on the calibration history.

6 Linearization curve

As the Beer-Lambert law is not linear for high absorbance values, a linearization curve is
entered to compensate automatically this non-linearity. This button display the linearization
curve and enable to enter or check the linearization values.

The Y-axis corresponds to the rough measurements entered on the M1 to M10 fields while the
X-axis corresponds to the standard or final measurement, entered on the S1 to S10 fields.

The M1-M10 and S1-S10 values must be strictly increasing. It’s recommended to put 0.0 and
0.0 as starting values for S1 and M1. The final value is linearly extrapolated between these
points. Unused points at the end of the table must strictly remains at 0.0 both for S and M.

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Recommendations for recalibration

The analyser is factory calibrated. However, periodical checking is recommended and a

recalibration might be necessary after several months depending of the conditions of use.

To recalibrate the analyser, proceed as follow:

- Prepare a 50 mg/l NO3 standard. For this weight 1.629 g of dried KNO3 and dilute in 1litre of
distilled water to obtain a mere solution of 1g/l. Then take 50 ml of this mere solution and
complete to 1 litre of distilled water.

- Do a manual measurement on this standard using the check screen (as describe above).

- Go on the calibration screen and press on the “ADJUST” button of the check screen. Validate
the previous measurement and then enter the standard value on the keypad.

The calibration is finished. A new calibration factor has been calculated and has been recorded
inside the calibration history displayed on the check screen.

This new calibration factor will be taken in account for all the further measurements.

4.9.5 Maintenance and troubleshooting

Maintenance

The maintenance is limited to refilling of the 5% H2SO4 cleaning solution.

Troubleshooting

Symptoms Origin

Negative value - Bad zero or auto-zero: the cleaning solution has been prepared with
polluted water or fault in the acid cleaning system (no flow, pump
failure)

Value is too low - No water in the flow cell (clogging, bad inlet connection, fault in the
peristaltic pump)

- Bad zero or auto-zero: cleaning solution has been prepared with

polluted water or fault in the acid cleaning system (no flow, pump
failure)

- Bad calibration: check or redo a calibration

Value is too high - Bad calibration: check or redo the calibration.

Unstable value - Deposit or dirty on the flow cell, check the light level on the check
screen

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- Moving optical part (check manually after transportation).

- Bad calibration, check with a standard.

Measuring errors

Error Signification Origin / Remediation

no

1 No signal at all from - Peak photo detector PH1 disconnected or failure

the peak photo
detector PH1

2 No signal at all from - Reference photo detector PH2 disconnected or failure

the reference photo
detector PH2

3 The light level is too - Check that there is no bubble inside the flow cell, if yes check
high on the peak the fittings
photo detector PH1
- Failure on the photo detector board or cable (repair or
replace)

4 The light level is too - Check that there is no bubble inside the flow cell, if yes check
high on the reference the fittings
photo detector PH2
- Failure on the photo detector board or cable (repair or
replace)

5 The light level is too - Over range or very high turbidity. Recheck on pure water
low on the peak
photo detector PH1 - Dirty on the flow cell windows. Check if the automatic
cleaning system is working properly

- Lamp failure or lamp supply board failure. Check if flashes

are visible during the measurement. Check the neon indicator
N1 on the XENON400 board.

6 The light level is too - Over range or very high turbidity. Recheck on pure water
low on the reference
photo detector PH2 - Dirty on the flow cell windows. Check if the automatic
cleaning system is working properly

- Lamp failure or lamp supply board failure. Check if flashes

are visible during the measurement. Check the neon indicator
N1 on the XENON400 board that must be always lighted.

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4.9.6 Specifications

Specifications

Calibrated range: 0 - 100 mg/l NO3

Measuring range: 0 - 150 mg/l NO3

Measuring time: 5 seconds

Accuracy: +/- 0.1 mg/l NO3 or +/- 5% whichever is greater

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4.10 Nitrite (NO2)

4.10.1 Principle

The measuring principle is based on the Azo dye (US-EPA 353.3) colorimetric method. A small
quantity of reagent and buffer are injected on the sample. After a fixed reaction time, the
developed colour is measured by the light absorption according to the Beer-Lambert law:

[C ] = k ⋅ log I 0 
 I1 

With [C ] : Sample concentration

k: Absorption coefficient (molecule specific)
I0: Light intensity before the sample
I1 : Light intensity after of the sample

The light is generated by a green LED centred at 525 nm.

1 Tri-color LED 4 Reagent pump(s)

2 Flow cell 5 Reagent tank(s)
3 Photo detector

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4.10.2 Settings

The settings are display on the two following screens.

First screen:

1 Label

This field allows changing the channel label displayed with the measurement. The label size is
limited to 6 characters.

2 Unit

This field allows changing the channel unit displayed with the measurement. The unit size is
limited to 6 characters.

3 High alarm value

The high alarm level can be changed by pressing on this field. A default value is preset when
the channel is created. The high alarm level is primary used to fix the high limit represented by a
red line on the recorded measurement graph. The limit is used as the minimum graph scale.

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The secondary use is to drive a high alarm relay if the relay button has been activated and if a
relay module has been installed on the selected module socket. In this case, the relay will be
activated as soon as the measurement overpass the high alarm value and will stay activated
until the measurement goes down below this value after subtracting the boundary value.

Refer to installation section for the relay wiring. Both functions NO (Normally Open) and NC
(Normally Closed) are available.

4 Low alarm value

The low alarm level can be changed by pressing on this field. A default value is preset when the
channel is created. The low alarm level is primary used to fix the low limit represented by a
green line on the recorded measurement graph.

The secondary use is to drive a low alarm relay if the relay button has been activated and if a
relay module has been installed on the selected module socket. In this case, the relay will be
activated as soon as the measurement underpass the low alarm value and will stay activated
until the measurement goes down below this value after adding the boundary value.

Refer to installation section for the relay wiring. Both functions NO (Normally Open) and NC
(Normally Closed) are available.

5 Number of decimals

This field allows changing the number of decimal displayed for the measurement value to adjust
the display to significant decimals only.

Note: this choice does not affect the measurements stored in the internal memory or the
MODBUS registers.

6 Negative values displayed

Negatives values are normal for most of the measurements as they result from normal
fluctuations if the measurement is close to zero.

By default, the display of negative values is allowed as negative values may also result from a
wrong zeroing. Consequently, it may be useful to be aware of such problem when seeing
strongly negative values.

But as negative values have no theoretical meaning, it’s possible to replace them by zero by
selecting "NO" for this function.

Note that this function do not apply to the recorded measurements or to the MODBUS registers.

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Second screen:

1 Scale factor

This field allows changing the scale factor of the channel.

Precautions must be taken before changing this value.

This factor is normally determined in factory to transform the basic measurement to a scaled
value in the final unit. It is has no reason to be changed except if optical elements are replaced.

2 Averaging

This field displays the number of elemental acquisitions averaged to form one measurement.
The default value is 50.

3 Sampling time

This field displays the sampling time specifically for this measurement.
A minimum time of 10 sec is recommended to allow a good renew of the sample.
It might be longer depending on the length and height of tubing to take the sample.

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4 Reagent time

This field displays the reagent time injection. The default value is 1 sec.
Shorter time may produce instable measurements or limiting the measuring range.
Longer time are not necessary and generate a waste of reagent.

5 Mixing delay or reaction time

This field displays the colorimetric reaction time. The default value is 120 sec and this delay is in
accordance with the standard method.
Shorter times may reduce the measurement stability, especially for low temperatures.

6 Waste time

This field displays the purge time after the measurement. The default value is 5 sec.
The waste can be collected during this time if a relay module declared with the "waste" function
is installed.

7 Temperature coefficient

The colorimetric reaction might be slightly affected by the temperature. This coefficient is used
for the automatic temperature compensation.

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Third screen:

1 Compensation

This button allows to compensate the sample turbidity. It's mandatory for turbid sample,
otherwise the measuring value should be underestimated or even negative.

2 LED selection

This field display the LED chosen for the measurement. Never change it, unless the colorimetric
method is changed.

3 Minimum graph Y scale

This field fix the minimum Y scale of the reaction curve.

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4.10.3 Test

The check screen displays the last measurement performed, whatever manual or automatic,
with the reaction curve.

5 6 7 8 9 10

1 Reaction curve

This curve corresponds to the last reaction curve, or to the current reaction during a measuring
cycle. Intermediate measurements are performed every 10 sec. The final measurement is the
average of the last 5 intermediate measurements.

2 Final value

This value in black corresponds to the final value of the last measuring cycle. It is display in grey
during a measuring cycle and correspond to the intermediate measurements.

3 Reference signal

This value corresponds to the reference signal before the reagent injection.
Typical values are between 1000 and 3000. It may be lower in case of turbid sample.

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4 Current signal

This value correspond to the current signal level during the measuring cycle, or to the final
signal value when finished.

5 Start cycle button

This button allows to start a new measuring cycle.

6 Stop button

This button stop an engaged measuring cycle or a running pump.

7 Sampling pump button

This button run the sampling pump for 10 seconds, for example to purge the circuit or the check
the sampling pump.

8 Reagent pump button

This button run the reagent pump for 10 seconds, for example to fill the reagent circuit during
the first installation.

9 Signal test button

This button display the LED signal. It should normally be between 1000 and 3000 on pure
water. The screen is shown below.

10 Test button

This button is reserved for factory testings.

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Signal screen:

4.10.4 Calibration

The calibration screen below enable to recalibrate the measurement channel.

See after this screen description the recommendations for recalibration.

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1 Last calibration window

This window displays the 10 last calibrations done with the date, time and the new calibration
factor.

2 Scan last calibrations

These two buttons allow to scroll up and down the 10 last calibration records.

3 Calibration factor

This field allows changing manually the calibration factor of the channel.
This factor is normally changed automatically while doing a calibration procedure by pressing on
the “ADJUST” button.

4 Offset

This field shows the internal offset applied for zeroing.

It is updated at each zeroing and has normally not to be changed.

5 Calibration procedure

This button start a calibration procedure.

The last measured value is displayed and must be validated.
Then the standard value must be entered.
When finished, a new calibration factor is determined and recorded on the calibration history.

6 Linearization curve

As the Beer-Lambert law is not linear for high absorbance values, a linearization curve is
entered to compensate automatically this non-linearity. This button display the linearization
curve and enable to enter or check the linearization values.

The Y-axis corresponds to the rough measurements entered on the M1 to M10 fields while the
X-axis corresponds to the standard or final measurement, entered on the S1 to S10 fields.

The M1-M10 and S1-S10 values must be strictly increasing. It’s recommended to put 0.0 and
0.0 as starting values for S1 and M1. The final value is linearly extrapolated between these
points. Unused points at the end of the table must strictly remains at 0.0 both for S and M.

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Recommendations for recalibration

The analyser is factory calibrated. However, periodical checking is recommended and a

recalibration might be necessary after several months depending of the conditions of use.

To recalibrate the analyser, proceed as follow:

- Prepare a 1 mg/l NO2 standard.

For this weight 150 mg of sodium nitrite (NaNO2) and dilute in 1litre of distilled water to obtain a
mere solution of 0.1g/l NO2

Then take 10 ml of this mere solution and complete to 1 litre of distilled water to get a 1 mg/l
NO2 standard.

- Do a manual measurement on this standard using the check screen (as describe above).

- Then go to the calibration screen and press on the “ADJUST” button. Validate the last
measured value and then enter the standard value on the keypad (1 mg/l).

The calibration is finished. A new calibration factor has been calculated and has been recorded
inside the calibration history displayed on the check screen.

This new calibration factor will be taken in account for all the further measurements.

4.10.5 Maintenance and troubleshooting

Maintenance

The maintenance is limited to refilling of the two reagent bottles and the 5% H2SO4 cleaning
solution.

Troubleshooting

Symptoms Origin

Negative value - Very high turbidity on the sample that is not compensated

Activate the polynomial compensation on the 3rd screen of

parameters.

Value is too low - No water in the flow cell (clogging, bad inlet connection, fault in the
peristaltic pump)

- No reagent or fault in the reagent injection (pump failure, tube

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pinched or clogged)

- Bad calibration: check or redo calibration.

Value is too high - Bad calibration: check or redo a calibration.

- Interferences in the sample

Unstable value - Deposit or dirty on the flow cell, check the light level on the check
screen with pure water

- Moving optical part (check manually after transportation).

- Bad calibration, check with a standard.

Measuring errors

Error Signification Origin / Remediation

no

1 No signal at all - Photo detector disconnected or faulty

2 The light level is too - Check that there is no bubble inside the flow cell, if yes check
high the fittings

- Failure on the photo-detector board

3 The light level is too - Sample concentration over range, check again with pure
low water

- Sample turbidity too high, check again with pure water

- Deposit or dirty on the flow cell

- Failure on the LED board

4 Alarm for no reagent - Refill the reagent tank

- Check the level detector connection if any

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4.10.6 Specifications

Specifications for the nitrite with Azo dye method

Calibrated range: 0 – 1 mg/l NO2

Measuring range: 0 – 4 mg/l NO2

Measuring time: 120 seconds

Accuracy: +/- 0.03 mg/l or +/- 5% whichever is greater

Interferences

Known as interfering because of precipitation: Sb3+, Au3+, Bi3+, Fe3+, Pb2+, Hg2+, Ag+,
chloroplatinate (PtCl6), and metavanadate (VO3).

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4.11 ORP (Oxidation-reduction potential)

4.11.1 Principle

The reduction potential is a measure of the tendency of the solution to either gain or lose
electrons when it is subject to change by introduction of a new species.

A solution with a higher (more positive) reduction potential than the new species will have a
tendency to gain electrons from the new species (i.e. to be reduced by oxidizing the new
species) and a solution with a lower (more negative) reduction potential will have a tendency to
lose electrons to the new species (i.e. to be oxidized by reducing the new species).

The method of measurement is based on the potential between two half-cells (reference and
sensing) containing a conductor immersed in an appropriate electrolyte solution and ended by a
conductive glass membrane. These two half-cells are generally combined in a single body
electrode.

1 Internal electrode 4 Internal electrolyte solution

2 Reference electrode 5 Glass bulb
3 Reference electrolyte solution

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4.11.2 Settings

The settings are display on the two following screens.

First screen:

1 Label

This field allows changing the channel label displayed with the measurement. The label size is
limited to 6 characters.

2 Unit

This field allows changing the channel unit displayed with the measurement. The unit size is
limited to 6 characters.

3 High alarm value

The high alarm level can be changed by pressing on this field. A default value is preset when
the channel is created. The high alarm level is primary used to fix the high limit represented by a
red line on the recorded measurement graph. The limit is used as the minimum graph scale.

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The secondary use is to drive a high alarm relay if the relay button has been activated and if a
relay module has been installed on the selected module socket. In this case, the relay will be
activated as soon as the measurement overpass the high alarm value and will stay activated
until the measurement goes down below this value after subtracting the boundary value.

Refer to installation section for the relay wiring. Both functions NO (Normally Open) and NC
(Normally Closed) are available.

4 Low alarm value

The low alarm level can be changed by pressing on this field. A default value is preset when the
channel is created. The low alarm level is primary used to fix the low limit represented by a
green line on the recorded measurement graph.

The secondary use is to drive a low alarm relay if the relay button has been activated and if a
relay module has been installed on the selected module socket. In this case, the relay will be
activated as soon as the measurement underpass the low alarm value and will stay activated
until the measurement goes down below this value after adding the boundary value.

Refer to installation section for the relay wiring. Both functions NO (Normally Open) and NC
(Normally Closed) are available.

5 Number of decimals

This field allows changing the number of decimal displayed for the measurement value to adjust
the display to significant decimals only.

Note: this choice does not affect the measurements stored in the internal memory or the
MODBUS registers.

6 Negative values displayed

Negatives values are normal for most of the measurements as they result from normal
fluctuations if the measurement is close to zero.

By default, the display of negative values is allowed as negative values may also result from a
wrong zeroing. Consequently, it may be useful to be aware of such problem when seeing
strongly negative values.

But as negative values have no theoretical meaning, it’s possible to replace them by zero by
selecting "NO" for this function.

Note that this function do not apply to the recorded measurements or to the MODBUS registers.

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Second screen:

1 Scale factor

This field allows changing the scale factor of the channel.

Precautions must be taken before changing this value.

This factor is normally determined in factory to transform the basic measurement to a scaled
value in the final unit. It is has no reason to be changed except if elements are replaced.

2 Temperature adjustment

The temperature of the sample given by the internal temperature probe can be adjusted by this
field if it differs from the real temperature read on a accurately calibrated thermometer.

This adjustment is not really important as a difference of temperature is taken in account during
the calibration. The adjustment is supposed to stay within +/- 4 °C.

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4.11.3 Test

1 Read value

This value is continuously updated. It may be compared to a standard solution.

2 Signal test

This button is used to display the two analogue-digital converters of the conductivity module for
testing purpose only and is reserved to the manufacturer.

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4.11.4 Calibration

The calibration screen below enable to recalibrate the measurement channel.

See after this screen description the recommendations for recalibration.

1 Last calibration window

This window displays the 10 last calibrations done with the date, time and the new calibration
factor.

2 Scan last calibrations

These two buttons allow to scroll up and down the 10 last calibration records.

3 Calibration factor

This field allows changing manually the calibration factor of the channel.

This factor is normally changed automatically while doing the second point calibration.

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4 Offset

This field shows the internal offset applied.

It is updated while doing the zero calibration point and has normally not to be changed.

5 Calibration procedure

This button start a calibration procedure.

The last measured value is displayed and must be validated.
Then the standard value must be entered.
When finished, a new calibration factor is determined and recorded on the calibration history.

Recommendations for recalibration

The ORP electrode must be checked on a regular base depending on the condition of use, and
recalibrated if necessary.

To recalibrate the electrode, proceed as follow:

- Put the electrode in a zero buffer solution (or alternatively replace the electrode by a strap)

- Do manual measurement on this buffer using the check screen (as describe above), wait for
the stabilisation of the ORP value, then press on the ZERO key.

- Put the electrode in a standard buffer solution

- Do a manual measurement on this standard using the check screen (as describe above) and
wait for the stabilisation of the ORP value

- Then go to the calibration screen and press on the “ADJUST” button. Validate the last
measured value and then enter the standard value on the keypad.

The calibration is finished. New offset and calibration factor has been calculated and has been
recorded in the calibration history. These new offset and calibration factor will be taken in
account for all the further measurements.

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6.11.5 ORP maintenance and troubleshooting

Maintenance

The pH probe must be cleaned on a regular base (generally weekly) depending of the
application. It must be replace about every 6 months (depending of the application).

Troubleshooting

Symptoms Origin

Negative value - Bad calibration

- ORP probe disconnected

Value is too low - Bad calibration: check or redo a calibration

Value is too high - Bad calibration: check or redo a calibration.

Unstable value - Electrical interference on the probe cable, put the probe cable away
from power cables

- Dirty on the electrode

- Aging electrode (replace)

6.11.6 ORP specifications

Measuring range: - 2000 mV to +2000 mV

Measuring time: 2 seconds

Accuracy: +/- 1 mV or +/- 5% whichever is greater

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4.12 pH

4.12.1 Principle

The pH of a solution is the negative logarithm of the molar concentration of dissolved hydronium
ions (H3O+).

The method of measurement is based on the potential between two half-cells (reference and
sensing) containing a conductor immersed in an appropriate electrolyte solution and ended by a
conductive glass membrane. These two half-cells are generally combined in a single body
electrode.
The voltage between the half-cell follows the Nernst equation:

V = V0 + 2,3
RT [
H +m
⋅ log +
]
nF [H ref ]
With V0: voltage of the reference half-cell, R: ideal gas constant, T: absolute temperature, n
:valence, F: Faraday constant, H+m: concentration of H+ in the measured solution, H+ref:
reference concentration of H+.

At 25ºC, one pH unit corresponds to 59.16 mV. At pH 7, the output is 0V (for all temperatures).

As pH depends of temperature, a temperature sensor is generally include in the electrode (100

Ohm or 1000 Ohm Platinum resistor) and connected to the instrument for automatic
temperature compensation (ATC).

1 Internal electrode 4 Internal electrolyte solution

2 Reference electrode 5 Glass bulb
3 Reference electrolyte solution

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4.12.2 Settings

The settings are display on the two following screens.

First screen:

1 Label

This field allows changing the channel label displayed with the measurement. The label size is
limited to 6 characters.

2 Unit

This field allows changing the channel unit displayed with the measurement. The unit size is
limited to 6 characters.

3 High alarm value

The high alarm level can be changed by pressing on this field. A default value is preset when
the channel is created. The high alarm level is primary used to fix the high limit represented by a
red line on the recorded measurement graph. The limit is used as the minimum graph scale.

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The secondary use is to drive a high alarm relay if the relay button has been activated and if a
relay module has been installed on the selected module socket. In this case, the relay will be
activated as soon as the measurement overpass the high alarm value and will stay activated
until the measurement goes down below this value after subtracting the boundary value.

Refer to installation section for the relay wiring. Both functions NO (Normally Open) and NC
(Normally Closed) are available.

4 Low alarm value

The low alarm level can be changed by pressing on this field. A default value is preset when the
channel is created. The low alarm level is primary used to fix the low limit represented by a
green line on the recorded measurement graph.

The secondary use is to drive a low alarm relay if the relay button has been activated and if a
relay module has been installed on the selected module socket. In this case, the relay will be
activated as soon as the measurement underpass the low alarm value and will stay activated
until the measurement goes down below this value after adding the boundary value.

Refer to installation section for the relay wiring. Both functions NO (Normally Open) and NC
(Normally Closed) are available.

5 Number of decimals

This field allows changing the number of decimal displayed for the measurement value to adjust
the display to significant decimals only.

Note: this choice does not affect the measurements stored in the internal memory or the
MODBUS registers.

6 Boundaries

The purpose of boundary is avoiding too frequent changes of state of the alarm relays what can
harm process devices like pumps or electric-valves. The boundary value is subtracted from the
high level value before leaving the high alarm state and reciprocally added to the low alarm level
before leaving the low alarm state. More details including a schematic are provided in the relay
section.

7 Negative values displayed

Negatives values are normal for most of the measurements as they result from normal
fluctuations if the measurement is close to zero.

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By default, the display of negative values is allowed as negative values may also result from a
wrong zeroing. Consequently, it may be useful to be aware of such problem when seeing
strongly negative values.

But as negative values have no theoretical meaning, it’s possible to replace them by zero by
selecting "NO" for this function.

Note that this function do not apply to the recorded measurements or to the MODBUS registers.

Second screen:

1 Scale factor

This field allows changing the scale factor of the channel.

Precautions must be taken before changing this value.

This factor is normally determined in factory to transform the basic measurement to a scaled
value in the final unit. It is has no reason to be changed except if elements are replaced.

2 Temperature adjustment

The temperature of the sample given by the internal temperature probe can be adjusted by this
field if it differs from the real temperature read on a accurately calibrated thermometer.

This adjustment is not really important as a difference of temperature is taken in account during
the calibration. The adjustment is supposed to stay within +/- 4 °C.

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4.12.3 Test

1 Read value

This value is continuously updated. It may be compared to a standard solution.

2 Probe temperature

This value is the sample temperature given by the probe. If the probe temperature sensor is not
connected, this value remains fixed at 25.0 °C.

3 Signal test

This button is used to display the two analogue-digital converters of the conductivity module for
testing purpose only and is reserved to the manufacturer.

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4.12.4 Calibration

The calibration screen below enable to recalibrate the measurement channel.

See after this screen description the recommendations for recalibration.

1 Last calibration window

This window displays the 10 last calibrations done with the date, time and the new calibration
factor.

2 Scan last calibrations

These two buttons allow to scroll up and down the 10 last calibration records.

3 Calibration factor

This field allows changing manually the calibration factor of the channel.

This factor is normally changed automatically while doing the second point calibration.

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4 Offset

This field shows the internal offset applied.

It is updated while doing the pH 7 calibration point and has normally not to be changed.

5 pH 7 calibration point

This button enable to calibrate the pH electrode with a pH 7 buffer.

The current pH value is displayed. After stabilisation, press on the key "SET TO 7.0".
After this, the displayed value must be 7.0.

The offset is adjusted by this first calibration point.

6 pH 4 calibration point

This button enable to calibrate the pH electrode with a pH 4 buffer.

The current pH value is displayed. After stabilisation, press on the key "SET TO 4.0".
After this, the displayed value must be 4.0.

The calibration factor is adjusted by this second calibration point.

Recommendations for recalibration

The pH electrode must be checked on a regular base depending on the condition of use, and
recalibrated if necessary.

To recalibrate the electrode, proceed as follow:

- Put the electrode in a pH 7.0 buffer solution

- Press on the "pH=7" key of the calibration screen. Wait for the stabilisation of the pH value,
then press on the "SET TO 7.0" key.

- Put the electrode in a pH 4.0 buffer solution

- Press on the "pH=7" key of the calibration screen. Wait for the stabilisation of the pH value,
then press on the "SET TO 7.0" key.

The calibration is finished. New offset and calibration factor has been calculated and has been
recorded in the calibration history. These new offset and calibration factor will be taken in
account for all the further measurements.

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6.12.5 pH maintenance and troubleshooting

Maintenance

The pH probe must be cleaned on a regular base (generally weekly) depending of the
application. It must be replace about every 6 months (depending of the application).

Troubleshooting

Symptoms Origin

Negative value - Bad calibration

- pH probe disconnected

Value is too low - Bad calibration: check or redo a calibration

Value is too high - Bad calibration: check or redo a calibration.

Unstable value - Electrical interference on the probe cable, put the probe cable away
from power cables

- Dirty on the electrode

- Aging electrode (replace)

Measuring errors

Error Signification Origin / Remediation

no

1 Over range - Probe disconnected

- Failure on the pH board (replace)

6.12.6 pH specifications

Measuring range: 0 to 14

Measuring time: 30 seconds

Accuracy: +/- 0.01 pH or +/- 5% whichever is greater

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4.13 Phosphate

4.13.1 Principle

The measuring principle is based on the vanadomolybdophosphoric acid colorimetric method for
the high range or on the ascorbic acid colorimetric method for the low range.

A small quantity of reagent is injected on the sample. After a fixed reaction time, the developed
colour is measured by the light absorption according to the Beer-Lambert law:

[C ] = k ⋅ log I 0 
 I1 

With [C ] : Sample concentration

k: Absorption coefficient (molecule specific)
I0: Light intensity before the sample
I1 : Light intensity after of the sample

The light is generated by a tri-colour LED depending of the method selected.

1 Tri-color LED 4 Reagent pump(s)

2 Flow cell 5 Reagent tank(s)
3 Photo detector

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4.13.2 Settings

The settings are display on the two following screens.

First screen:

1 Label

This field allows changing the channel label displayed with the measurement. The label size is
limited to 6 characters.

2 Unit

This field allows changing the channel unit displayed with the measurement. The unit size is
limited to 6 characters.

3 High alarm value

The high alarm level can be changed by pressing on this field. A default value is preset when
the channel is created. The high alarm level is primary used to fix the high limit represented by a
red line on the recorded measurement graph. The limit is used as the minimum graph scale.

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The secondary use is to drive a high alarm relay if the relay button has been activated and if a
relay module has been installed on the selected module socket. In this case, the relay will be
activated as soon as the measurement overpass the high alarm value and will stay activated
until the measurement goes down below this value after subtracting the boundary value.

Refer to installation section for the relay wiring. Both functions NO (Normally Open) and NC
(Normally Closed) are available.

4 Low alarm value

The low alarm level can be changed by pressing on this field. A default value is preset when the
channel is created. The low alarm level is primary used to fix the low limit represented by a
green line on the recorded measurement graph.

The secondary use is to drive a low alarm relay if the relay button has been activated and if a
relay module has been installed on the selected module socket. In this case, the relay will be
activated as soon as the measurement underpass the low alarm value and will stay activated
until the measurement goes down below this value after adding the boundary value.

Refer to installation section for the relay wiring. Both functions NO (Normally Open) and NC
(Normally Closed) are available.

5 Number of decimals

This field allows changing the number of decimal displayed for the measurement value to adjust
the display to significant decimals only.

Note: this choice does not affect the measurements stored in the internal memory or the
MODBUS registers.

6 Negative values displayed

Negatives values are normal for most of the measurements as they result from normal
fluctuations if the measurement is close to zero.

By default, the display of negative values is allowed as negative values may also result from a
wrong zeroing. Consequently, it may be useful to be aware of such problem when seeing
strongly negative values.

But as negative values have no theoretical meaning, it’s possible to replace them by zero by
selecting "NO" for this function.

Note that this function do not apply to the recorded measurements or to the MODBUS registers.

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Second screen:

1 Scale factor

This field allows changing the scale factor of the channel.

Precautions must be taken before changing this value.

This factor is normally determined in factory to transform the basic measurement to a scaled
value in the final unit. It is has no reason to be changed except if optical elements are replaced.

2 Averaging

This field displays the number of elemental acquisitions averaged to form one measurement.
The default value is 50.

3 Sampling time

This field displays the sampling time specifically for this measurement.
A minimum time of 10 sec is recommended to allow a good renew of the sample.
It might be longer depending on the length and height of tubing to take the sample.

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4 Reagent time

This field displays the reagent time injection. The default value is 1 sec.
Shorter time may produce instable measurements or limiting the measuring range.
Longer time are not necessary and generate a waste of reagent.

5 Mixing delay or reaction time

This field displays the colorimetric reaction time. The default value is 120 sec and this delay is in
accordance with the standard method.
Shorter times may reduce the measurement stability, especially for low temperatures.

6 Waste time

This field displays the purge time after the measurement. The default value is 5 sec.
The waste can be collected during this time if a relay module declared with the "waste" function
is installed.

7 Temperature coefficient

The colorimetric reaction might be slightly affected by the temperature. This coefficient is used
for the automatic temperature compensation.

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Third screen:

1 Compensation

This button allows to compensate the sample turbidity. It's mandatory for turbid sample,
otherwise the measuring value should be underestimated or even negative.

2 LED selection

This field display the LED chosen for the measurement. Never change it, unless the colorimetric
method is changed.

3 Minimum graph Y scale

This field fix the minimum Y scale of the reaction curve.

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4.13.3 Test

The check screen displays the last measurement performed, whatever manual or automatic,
with the reaction curve.

5 6 7 8 9 10

1 Reaction curve

This curve corresponds to the last reaction curve, or to the current reaction during a measuring
cycle. Intermediate measurements are performed every 10 sec. The final measurement is the
average of the last 5 intermediate measurements.

2 Final value

This value in black corresponds to the final value of the last measuring cycle. It is display in grey
during a measuring cycle and correspond to the intermediate measurements.

3 Reference signal

This value corresponds to the reference signal before the reagent injection.
Typical values are between 1000 and 3000. It may be lower in case of turbid sample.

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4 Current signal

This value correspond to the current signal level during the measuring cycle, or to the final
signal value when finished.

5 Start cycle button

This button allows to start a new measuring cycle.

6 Stop button

This button stop an engaged measuring cycle or a running pump.

7 Sampling pump button

This button run the sampling pump for 10 seconds, for example to purge the circuit or the check
the sampling pump.

8 Reagent pump button

This button run the reagent pump for 10 seconds, for example to fill the reagent circuit during
the first installation.

9 Signal test button

This button display the LED signal. It should normally be between 1000 and 3000 on pure
water. The screen is shown below.

10 Test button

This button is reserved for factory testing.

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Signal screen:

4.13.4 Calibration

The calibration screen below enable to recalibrate the measurement channel.

See after this screen description the recommendations for recalibration.

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1 Last calibration window

This window displays the 10 last calibrations done with the date, time and the new calibration
factor.

2 Scan last calibrations

These two buttons allow to scroll up and down the 10 last calibration records.

3 Calibration factor

This field allows changing manually the calibration factor of the channel.
This factor is normally changed automatically while doing a calibration procedure by pressing on
the “ADJUST” button.

4 Offset

This field shows the internal offset applied for zeroing.

It is updated at each zeroing and has normally not to be changed.

5 Calibration procedure

This button start a calibration procedure.

The last measured value is displayed and must be validated.
Then the standard value must be entered.
When finished, a new calibration factor is determined and recorded on the calibration history.

6 Linearization curve

As the Beer-Lambert law is not linear for high absorbance values, a linearization curve is
entered to compensate automatically this non-linearity. This button display the linearization
curve and enable to enter or check the linearization values.

The Y-axis corresponds to the rough measurements entered on the M1 to M10 fields while the
X-axis corresponds to the standard or final measurement, entered on the S1 to S10 fields.

The M1-M10 and S1-S10 values must be strictly increasing. It’s recommended to put 0.0 and
0.0 as starting values for S1 and M1. The final value is linearly extrapolated between these
points. Unused points at the end of the table must strictly remains at 0.0 both for S and M.

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Recommendations for recalibration

The analyser is factory calibrated. However, periodical checking is recommended and a

recalibration might be necessary after several months depending of the conditions of use.

To recalibrate the analyser, proceed as follow:

- Prepare a 5 mg/l P-PO4 standard. For this weight 2.195 g of anhydrous monopotassium
phosphate (KH2PO4) and dilute in 1litre of distilled water to obtain a mere solution of 500 mg/l P-
PO4.

Then take 10 ml of this mere solution and complete to 1 litre of distilled water.

- Do a manual measurement on this standard using the check screen (as describe above).

- Then go to the calibration screen and press on the “ADJUST” button. Validate the last
measured value and then enter the standard value on the keypad (1 mg/l).

The calibration is finished. A new calibration factor has been calculated and has been recorded
inside the calibration history displayed on the check screen.

This new calibration factor will be taken in account for all the further measurements.

4.13.5 Maintenance and troubleshooting

Maintenance

The maintenance is limited to refilling of the two reagent bottles and the 5% H2SO4 cleaning
solution.

Troubleshooting

Symptoms Origin

Negative value - Very high turbidity on the sample that is not compensated

Activate the polynomial compensation on the 3rd screen of

parameters.

Value is too low - No water in the flow cell (clogging, bad inlet connection, fault in the
peristaltic pump)

- No reagent or fault in the reagent injection (pump failure, tube

pinched or clogged)

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- Bad calibration: check or redo calibration.

Value is too high - Bad calibration: check or redo a calibration.

- Interferences in the sample

Unstable value - Deposit or dirty on the flow cell, check the light level on the check
screen with pure water

- Moving optical part (check manually after transportation).

- Bad calibration, check with a standard.

Measuring errors

Error Signification Origin / Remediation

no

1 No signal at all - Photo detector disconnected or faulty

2 The light level is too - Check that there is no bubble inside the flow cell, if yes check
high the fittings

- Failure on the photo-detector board

3 The light level is too - Sample concentration over range, check again with pure
low water

- Sample turbidity too high, check again with pure water

- Deposit or dirty on the flow cell

- Failure on the LED board

4 Alarm for no reagent - Refill the reagent tank

- Check the level detector connection if any

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4.13.6 Specifications

Specifications for vanadomolybdophosphoric method

Calibrated range: 0 – 20 mg/l P-PO4

Measuring time: 180 seconds

Accuracy: +/- 0.05 mg/l P-PO4 or +/- 5% whichever is greater

Specifications for ascorbic acid method

Calibrated range: 0 – 2 mg/l P-PO4

Measuring time: 180 seconds

Accuracy: +/- 0.05 mg/l P-PO4 or +/- 5% whichever is greater

Interferences for vanadomolybdophosphoric method

Possible negative interferences from arsenate, fluoride, thorium, bismuth, sulfide,

thiosulfate, thiocyanate.

Ions that do not interfere up to 1000 mg/l:

Al3+, Fe3+, Mg2+, Ca2+, Ba2+, Sr2+, Li+, Na+, K+, NH4+, Cd2+, Mn2+,Pb2+, Hg+, Hg2+,
Sn2+, Cu2+, Ni2+, Ag+, U4+, Zr4+, AsO3–, Br–, CO32–, ClO4–, CN–, IO3–,SiO44–, NO3–,
NO2–, SO42–, O32–, pyrophosphate, molybdate, tetraborate, selenate, benzoate, citrate,
oxalate, lactate, tartrate, formate, and salicylate.

Interferences for ascorbic acid method

Arsenates interfere positively for concentration as low as 0.1 mg/l.

Hexavalent chromium and NO2 interfere negatively (about 3% low at

concentrations of 1 mg/L and 10 to 15% low at 10 mg/L).

Sulfide (Na2S) and silicate do not interfere at concentrations of 1.0 and 10 mg/L.

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4.14 Temperature

4.14.1 Principle

The temperature measurement is based on the resistance measurement of a standard platinum

element of 100 ohm or 1000 ohm at 0°C (Pt100 or Pt1000). This element is included inside the
pH or conductivity electrode.

The table below shows the Pt 100 resistance in ohm for temperature between 0°C and 100°C.

°C 0 1 2 3 4 5 6 7 8 9
0 100.00 100.39 100.78 101.17 101.56 101.95 102.34 102.73 103.12 103.51
10 103.90 104.29 104.68 105.07 105.46 105.85 106.24 106.63 107.02 107.40
20 107.79 108.18 108.57 108.96 109.35 109.73 110.12 110.51 110.90 111.28
30 111.67 112.06 112.45 112.83 113.22 113.61 113.99 114.38 114.77 115.15
40 115.54 115.93 116.31 116.70 117.08 117.47 117.85 118.24 118.62 119.01
50 119.40 119.78 120.16 120.55 120.93 121.32 121.70 122.09 122.47 122.86
60 123.24 123.62 124.01 124.39 124.77 125.16 125.54 125.92 126.31 126.69
70 127.07 127.45 127.84 128.22 128.60 128.98 129.37 129.75 130.13 130.51
80 130.89 131.27 131.66 132.04 132.42 132.80 133.18 133.56 133.94 134.32
90 134.70 135.08 135.46 135.84 136.22 136.60 136.98 137.36 137.74 138.12

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4.14.2 Settings

The settings are display on the two following screens.

First screen:

1 Label

This field allows changing the channel label displayed with the measurement. The label size is
limited to 6 characters.

2 Unit

This field allows changing the channel unit displayed with the measurement. The unit size is
limited to 6 characters.

3 High alarm value

The high alarm level can be changed by pressing on this field. A default value is preset when
the channel is created. The high alarm level is primary used to fix the high limit represented by a
red line on the recorded measurement graph. The limit is used as the minimum graph scale.

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The secondary use is to drive a high alarm relay if the relay button has been activated and if a
relay module has been installed on the selected module socket. In this case, the relay will be
activated as soon as the measurement overpass the high alarm value and will stay activated
until the measurement goes down below this value after subtracting the boundary value.

Refer to installation section for the relay wiring. Both functions NO (Normally Open) and NC
(Normally Closed) are available.

4 Low alarm value

The low alarm level can be changed by pressing on this field. A default value is preset when the
channel is created. The low alarm level is primary used to fix the low limit represented by a
green line on the recorded measurement graph.

The secondary use is to drive a low alarm relay if the relay button has been activated and if a
relay module has been installed on the selected module socket. In this case, the relay will be
activated as soon as the measurement underpass the low alarm value and will stay activated
until the measurement goes down below this value after adding the boundary value.

Refer to installation section for the relay wiring. Both functions NO (Normally Open) and NC
(Normally Closed) are available.

5 Number of decimals

This field allows changing the number of decimal displayed for the measurement value to adjust
the display to significant decimals only.

Note: this choice does not affect the measurements stored in the internal memory or the
MODBUS registers.

6 Boundaries

The purpose of boundary is avoiding too frequent changes of state of the alarm relays what can
harm process devices like pumps or electric-valves. The boundary value is subtracted from the
high level value before leaving the high alarm state and reciprocally added to the low alarm level
before leaving the low alarm state. More details including a schematic are provided in the relay
section.

7 Negative values displayed

Negatives values are normal for most of the measurements as they result from normal
fluctuations if the measurement is close to zero.

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By default, the display of negative values is allowed as negative values may also result from a
wrong zeroing. Consequently, it may be useful to be aware of such problem when seeing
strongly negative values.

But as negative values have no theoretical meaning, it’s possible to replace them by zero by
selecting "NO" for this function.

Note that this function do not apply to the recorded measurements or to the MODBUS registers.

Second screen:

1 Scale factor

This field allows changing the scale factor of the channel.

Precautions must be taken before changing this value.

This factor is normally determined in factory to transform the basic measurement to a scaled
value in the final unit. It is has no reason to be changed except if elements are replaced.

2 Temperature adjustment

The temperature of the sample given by the internal temperature probe can be adjusted by this
field if it differs from the real temperature read on a accurately calibrated thermometer.

The adjustment is supposed to stay within +/- 4 °C.

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4.14.3 Test

3 2

1 Read value

This value is continuously updated. It may be compared to a reference thermometer.

2 Zero button

This button is used for zeroing the measurement.

Put the electrode in a Dewar filled with ice and water and wait for stabilization before
pressing this button.

3 Signal test

This button is used to display the two analogue-digital converters of the conductivity module for
testing purpose only and is reserved to the manufacturer.

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4.14.4 Calibration

The calibration screen below enable to recalibrate the measurement channel.

See after this screen description the recommendations for recalibration.

1 Last calibration window

This window displays the 10 last calibrations done with the date, time and the new calibration
factor.

2 Scan last calibrations

These two buttons allow to scroll up and down the 10 last calibration records.

3 Calibration factor

This field allows changing manually the calibration factor of the channel.

This factor is normally changed automatically while doing a calibration procedure by pressing on
the “ADJUST” button.

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4 Offset

This field shows the internal offset applied for zeroing.

It is updated at each zeroing and has normally not to be changed.

5 Calibration procedure

This button start a calibration procedure.

The last measured value is displayed and must be validated.
Then the standard value must be entered.
When finished, a new calibration factor is determined and recorded on the calibration history.

6 Linearization curve

For more accuracy, a linearization curve can be entered. This button display the linearization
graph and enable to enter or check the linearization values.

Linearization is normally not necessary for temperature measurements.

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Recommendations for recalibration

The temperature probe is factory calibrated with an accuracy of +/- 1°C. It can be recalibrated if
a higher accuracy is requested.

To recalibrate the electrode, proceed as follow:

- Put the temperature probe in a Dewar filled of ice and water

- Do manual measurement using the check screen (as describe above), wait for the stabilisation
of the temperature value, and then press on the ZERO key.

- Put the temperature probe in a Dewar filled of water at a temperature between 50°C and 80°C
with a reference thermometer inside

- Do manual measurement using the check screen, wait for the stabilisation of the temperature
value

- Go to the calibration screen, then press on the ADJUST key. Validate the last measurement
then enter the value given by the reference thermometer on the keypad.

The calibration is finished. New offset and calibration factors has been calculated and has been
recorded inside the zero and calibration history displayed on the check screen.

These new calibration factors will be taken in account for all the further measurements.

4.4.5 Maintenance and troubleshooting

Maintenance

No maintenance is required.

Troubleshooting

Symptoms Origin

Value is too low - Bad calibration: check or redo a calibration

Value is too high - Bad calibration: check or redo a calibration.

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Measuring errors

Error Signification Origin / Remediation

no

1 Probe disconnected - Check the connection of the temperature probe

- Failure on the temperature module (replace)

2 Probe short-circuited - Check the probe with an ohmmeter, the value must be in a
100 to 120 ohm range, if not replace the probe

- Failure on the temperature module (replace)

4.14.6 Specifications

Specifications

Calibrated range: 0 - 50 °C

Measuring range: 0 - 100°C

Measuring time: 2 seconds

Accuracy: +/- 1 °C

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4.15 Turbidity by absorbance (external probe)

4.15.1 Principle

The measuring principle is based on the infra-red light absorbance at 880 nm over a defined
optical path.

Two models are available, both are designed for waste water:

- High range with a optical path of 6.4 mm (example biological reactor)

- Low range with an optical path of 25.4 mm (example clarifier or final effluent)

The detection system is placed at the end of an immersed probe in a robust PVC case.

An automatic cleaning of the windows can be done by sending compressed air on a regular
base.

1 LED source
2 Photo detector

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4.15.2 Settings

The settings are display on the two following screens.

First screen:

1 Label

This field allows changing the channel label displayed with the measurement. The label size is
limited to 6 characters.

2 Unit

This field allows changing the channel unit displayed with the measurement. The unit size is
limited to 6 characters.

3 High alarm value

The high alarm level can be changed by pressing on this field. A default value is preset when
the channel is created. The high alarm level is primary used to fix the high limit represented by a
red line on the recorded measurement graph. The limit is used as the minimum graph scale.

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The secondary use is to drive a high alarm relay if the relay button has been activated and if a
relay module has been installed on the selected module socket. In this case, the relay will be
activated as soon as the measurement overpass the high alarm value and will stay activated
until the measurement goes down below this value after subtracting the boundary value.

Refer to installation section for the relay wiring. Both functions NO (Normally Open) and NC
(Normally Closed) are available.

4 Low alarm value

The low alarm level can be changed by pressing on this field. A default value is preset when the
channel is created. The low alarm level is primary used to fix the low limit represented by a
green line on the recorded measurement graph.

The secondary use is to drive a low alarm relay if the relay button has been activated and if a
relay module has been installed on the selected module socket. In this case, the relay will be
activated as soon as the measurement underpass the low alarm value and will stay activated
until the measurement goes down below this value after adding the boundary value.

Refer to installation section for the relay wiring. Both functions NO (Normally Open) and NC
(Normally Closed) are available.

5 Number of decimals

This field allows changing the number of decimal displayed for the measurement value to adjust
the display to significant decimals only.

Note: this choice does not affect the measurements stored in the internal memory or the
MODBUS registers.

6 Modbus address

This field is the Modbus address of the probe that has been fixed inside the probe during the
initialisation sequence.
This value must not be changed, unless the RS485 scan screen indicates that the probe has not
the proper address.

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4.15.3 Test

The check screen displays measurement continuously.

1 Measured value

This value is continuously updated and may be compared to a standard.

Note that the response time at 90% is 60 seconds.

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4.15.4 Calibration

The calibration screen below enable to recalibrate the measurement channel.

See after this screen description the recommendations for recalibration.

4 5

1 Last calibration window

This window displays the 10 last calibrations done with the date, time and the new calibration
factor.

2 Scan last calibrations

These two buttons allow to scroll up and down the 10 last calibration records.

3 Calibration factor

This field allows changing manually the calibration factor of the channel.
This factor is normally changed automatically while doing a calibration procedure by pressing on
the “ADJUST” button.

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4 Zero

This button enable to dot the zero. Be sure that the probe is immersed on pure water before
pressing on this button.

5 Calibration procedure

This button start a calibration procedure.

The last measured value is displayed and must be validated.
Then the standard value must be entered.
When finished, a new calibration factor is determined and recorded on the calibration history.

Recommendations for recalibration

The analyser is factory calibrated. However, periodical checking is recommended and a

recalibration might be necessary after several months depending of the conditions of use.

To recalibrate the analyser, proceed as follow:

- Do a TSS laboratory measurement on a sample representative of the measuring range

- Do a manual measurement on the same sample using the check screen (as describe above).

- Go on the calibration screen and press on the “ADJUST” button of the check screen. Validate
or enter the measurement and then enter the laboratory value on the keypad.

The calibration is finished. A new calibration factor has been calculated and has been recorded
inside the calibration history displayed on the check screen.

This new calibration factor will be taken in account for all the further measurements.

4.15.5 Maintenance and troubleshooting

Maintenance

There is no special maintenance on this probe unless checking that the windows are clean.

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Troubleshooting

Symptoms Origin

Value is too low - Probe not immersed

- Wrong zero

- Bad calibration: check or redo a calibration

Value is too high - Bad calibration: check or redo a calibration.

Unstable value - Deposit or dirty on the windows

- Bad calibration, check with a standard.

Measuring errors

Error Signification Origin / Remediation

no

1 No connection - The probe is not connected or badly connected

- The probe is not properly configured (refer to the RS485

section for probe configuration).

A special initialisation must be done when the probe is

used for the first time

4.15.6 Specifications

Low range Specifications

Calibrated range: 0 - 1500 TSS

Measuring time: < 60 seconds for 90% of response

Accuracy: +/- 2 mg/l TSS or +/- 5% whichever is greater

High range Specifications

Calibrated range: 0 - 30 000 TSS

Measuring time: < 60 seconds for 90% of response

Accuracy: +/- 100 mg/l TSS or +/- 5% whichever is greater

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4.16 Turbidity by nephelometry with laser diode

4.16.1 Principle

The measuring principle is based on nephelometric method.

The light beam is generated by a pulsed laser diode while the light detection is performed by a
photodiode detector placed at 90° from the light beam as represented below.

Two models of laser diode are available: visible red at 655 nm or infrared at 850 nm.

The infra red model meet the IS07027 criteria. However, for the safety reasons and to ease the
troubleshooting, the visible red model is recommended.

Never observe directly the laser diode if dismounted. The infrared light might
damage the eye.

1 Laser diode source

2 Sample flow cell
3 Photo detector

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4.16.2 Settings

The settings are display on the two following screens.

First screen:

1 Label

This field allows changing the channel label displayed with the measurement. The label size is
limited to 6 characters.

2 Unit

This field allows changing the channel unit displayed with the measurement. The unit size is
limited to 6 characters.

3 High alarm value

The high alarm level can be changed by pressing on this field. A default value is preset when
the channel is created. The high alarm level is primary used to fix the high limit represented by a
red line on the recorded measurement graph. The limit is used as the minimum graph scale.

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The secondary use is to drive a high alarm relay if the relay button has been activated and if a
relay module has been installed on the selected module socket. In this case, the relay will be
activated as soon as the measurement overpass the high alarm value and will stay activated
until the measurement goes down below this value after subtracting the boundary value.

Refer to installation section for the relay wiring. Both functions NO (Normally Open) and NC
(Normally Closed) are available.

4 Low alarm value

The low alarm level can be changed by pressing on this field. A default value is preset when the
channel is created. The low alarm level is primary used to fix the low limit represented by a
green line on the recorded measurement graph.

The secondary use is to drive a low alarm relay if the relay button has been activated and if a
relay module has been installed on the selected module socket. In this case, the relay will be
activated as soon as the measurement underpass the low alarm value and will stay activated
until the measurement goes down below this value after adding the boundary value.

Refer to installation section for the relay wiring. Both functions NO (Normally Open) and NC
(Normally Closed) are available.

5 Number of decimals

This field allows changing the number of decimal displayed for the measurement value to adjust
the display to significant decimals only.

Note: this choice does not affect the measurements stored in the internal memory or the
MODBUS registers.

6 Negative values displayed

Negatives values are normal for most of the measurements as they result from normal
fluctuations if the measurement is close to zero.

By default, the display of negative values is allowed as negative values may also result from a
wrong zeroing. Consequently, it may be useful to be aware of such problem when seeing
strongly negative values.

But as negative values have no theoretical meaning, it’s possible to replace them by zero by
selecting "NO" for this function.

Note that this function do not apply to the recorded measurements or to the MODBUS registers.

Second screen:

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1 Scale factor

This field allows changing the scale factor of the channel.

Precautions must be taken before changing this value.

This factor is normally determined in factory to transform the basic measurement to a scaled
value in the final unit. It is has no reason to be changed except if optical elements are replaced.

2 Averaging

This field displays the number of elemental acquisitions averaged to form one measurement.
The default value is 50.

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4.16.3 Test

The check screen displays measurement continuously.

2 3 4 5 6 7

1 Measured value

This value is continuously updated and may be compared to a standard.

2 Sampling pump button

This button run the sampling pump for 10 seconds to renew the sample.

3 Cleaning pump button

This button injects the cleaning solution and may be used to check the zero.

4 Stop button

This button stop a running pump.

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5 Signal test button

This button display the laser diode signal. It should normally be below 300 on pure water. The
screen is shown below.

6 Zero button

This button is used for zeroing the measurement.

Put first pure water inside the flow cell before pressing this button.

7 Start measurement (unused)

Signal screen:

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4.16.4 Calibration

The calibration screen below enable to recalibrate the measurement channel.

See after this screen description the recommendations for recalibration.

1 Last calibration window

This window displays the 10 last calibrations done with the date, time and the new calibration
factor.

2 Scan last calibrations

These two buttons allow to scroll up and down the 10 last calibration records.

3 Calibration factor

This field allows changing manually the calibration factor of the channel.
This factor is normally changed automatically while doing a calibration procedure by pressing on
the “ADJUST” button.

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4 Offset

This field shows the internal offset applied for zeroing.

It is updated at each zeroing and has normally not to be changed.

5 Calibration procedure

This button start a calibration procedure.

The last measured value is displayed and must be validated.
Then the standard value must be entered.
When finished, a new calibration factor is determined and recorded on the calibration history.

6 Linearization curve

As the Beer-Lambert law is not linear for high absorbance values, a linearization curve is
entered to compensate automatically this non-linearity. This button display the linearization
curve and enable to enter or check the linearization values.

The Y-axis corresponds to the rough measurements entered on the M1 to M10 fields while the
X-axis corresponds to the standard or final measurement, entered on the S1 to S10 fields.

The M1-M10 and S1-S10 values must be strictly increasing. It’s recommended to put 0.0 and
0.0 as starting values for S1 and M1. The final value is linearly extrapolated between these
points. Unused points at the end of the table must strictly remains at 0.0 both for S and M.

Recommendations for recalibration

The analyser is factory calibrated. However, periodical checking is recommended and a

recalibration might be necessary after several months depending of the conditions of use.

To recalibrate the analyser, proceed as follow:

- Take a turbidity standard (formazine for example) representative of the measuring range

- Do a manual measurement on this standard using the check screen (as describe above).

- Go on the calibration screen and press on the “ADJUST” button of the check screen. Validate
the previous measurement and then enter the standard value on the keypad.

The calibration is finished. A new calibration factor has been calculated and has been recorded
inside the calibration history displayed on the check screen.

This new calibration factor will be taken in account for all the further measurements.

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4.16.5 Maintenance and troubleshooting

Maintenance

The maintenance is limited to refilling of the 5% H2SO4 cleaning solution.

Troubleshooting

Symptoms Origin

Negative value - Bad zero or auto-zero: the cleaning solution has been prepared with
polluted water or fault in the acid cleaning system (no flow, pump
failure)

Value is too low - No water in the flow cell (clogging, bad inlet connection, fault in the
peristaltic pump)

- Bad zero or auto-zero: cleaning solution has been prepared with

polluted water or fault in the acid cleaning system (no flow, pump
failure)

- Bad calibration: check or redo a calibration

Value is too high - Bubbles in the sample (check peristaltic pump inlet fitting).

- Bad calibration: check or redo a calibration.

Unstable value - Deposit or dirty on the flow cell, check the light level on the check
screen

- Moving optical part (check manually after transportation).

- Bad calibration, check with a standard.

Measuring errors

Error Signification Origin / Remediation

no

1 No signal at all from - Photo detector disconnected or failure

the photo detector

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2 The light level is too - Check that there is no bubble inside the flow cell, if yes check
high the fittings

- Failure on the photo detector board or cable (repair or

replace)

4.16.6 Specifications

Specifications

Calibrated range: 0 - 10 NTU

Measuring range: 0 - 100 NTU

Measuring time: 5 seconds

Accuracy: +/- 0.01 NTU or +/- 5% whichever is greater

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4.17 UV254

4.17.1 Principle

The measuring principle is based on the UV light absorption from organic matter at 254 nm
according to the Beer-Lambert law:

[C ] = k ⋅ log I 0 
 I1 

With [C ] : Sample concentration

k: Absorption coefficient (molecule specific)
I0: Light intensity before the sample
I1 : Light intensity after of the sample

The UV light is generated by a pulsed xenon lamp to reach an ultra long life time.

The light detection is performed by two photodiode detectors through interferential filters; one
centred at 254 nm while the other is used as reference to compensate turbidity, deposit inside
the flow cell and organic matter.

1 Xenon flash lamp

2 Sample flow cell
3 Beam splitter
4 Peak interferential filter
5 Peak photo detector
6 Reference interferential filter
7 Reference photo detector

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4.17.2 Settings

The settings are display on the two following screens.

First screen:

1 Label

This field allows changing the channel label displayed with the measurement. The label size is
limited to 6 characters.

2 Unit

This field allows changing the channel unit displayed with the measurement. The unit size is
limited to 6 characters.

3 High alarm value

The high alarm level can be changed by pressing on this field. A default value is preset when
the channel is created. The high alarm level is primary used to fix the high limit represented by a
red line on the recorded measurement graph. The limit is used as the minimum graph scale.

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The secondary use is to drive a high alarm relay if the relay button has been activated and if a
relay module has been installed on the selected module socket. In this case, the relay will be
activated as soon as the measurement overpass the high alarm value and will stay activated
until the measurement goes down below this value after subtracting the boundary value.

Refer to installation section for the relay wiring. Both functions NO (Normally Open) and NC
(Normally Closed) are available.

4 Low alarm value

The low alarm level can be changed by pressing on this field. A default value is preset when the
channel is created. The low alarm level is primary used to fix the low limit represented by a
green line on the recorded measurement graph.

The secondary use is to drive a low alarm relay if the relay button has been activated and if a
relay module has been installed on the selected module socket. In this case, the relay will be
activated as soon as the measurement underpass the low alarm value and will stay activated
until the measurement goes down below this value after adding the boundary value.

Refer to installation section for the relay wiring. Both functions NO (Normally Open) and NC
(Normally Closed) are available.

5 Number of decimals

This field allows changing the number of decimal displayed for the measurement value to adjust
the display to significant decimals only.

Note: this choice does not affect the measurements stored in the internal memory or the
MODBUS registers.

6 Mode

This button allows to choose between the Abs/m mode or the COD mode.

The Abs/m mode is the absolute measuring mode, giving directly the absorbance of the sample
at 254 nm for a one meter optical path, compensated at 350 nm to remove the turbidity effect.

The COD mode is based on an experimental correlation between the UV absorbance at 254 nm
and the COD (Chemical Oxygen Demand).

This correlation is excellent for river water and drinking water and generally good for food
industries and paper mills.

Precautions must be taken for chemical industries effluent as the UV254/COD ratio
may depends on the process, each molecule having a specific UV245/COD ratio.
Mixing different kind of effluent might give inaccurate or inconsistent results.

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Some saturated organic molecules as glucose or alcohol do not have UV light

absorption and consequently cannot be detected by UV absorbance.

The linearization curve is applied only in COD mode as the absorbance is inherently
linear.

7 Negative values displayed

Negatives values are normal for most of the measurements as they result from normal
fluctuations if the measurement is close to zero.

By default, the display of negative values is allowed as negative values may also result from a
wrong zeroing. Consequently, it may be useful to be aware of such problem when seeing
strongly negative values.

But as negative values have no theoretical meaning, it’s possible to replace them by zero by
selecting "NO" for this function.

Note that this function do not apply to the recorded measurements or to the MODBUS registers.

Second screen:

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1 Scale factor

This field allows changing the scale factor of the channel.

Precautions must be taken before changing this value.

The default scale factor is fixed according to the flow cell optical path to transform the
absorbance to absorbance by meter:

- Flow cell of 10 mm: 100

- Flow cell of 3 mm: 333.3
- Flow cell of 1 mm: 1000

2 Averaging

This field displays the number of elemental acquisitions averaged to form one measurement.
The default value is 200.

3 Flow cell size

This button must be selected depending on the flow cell optical path that represents the
distance between the two quartz cylinders visible inside the flow cell.

No change must be done unless the flow cell is changed. The scale factor is automatically set
according the flow cell size.

The size of the flow cell is factory selected depending of the application:

- 10 mm: drinking water, low turbidity river water and low turbidity effluents
- 3 mm: turbid river water and turbid effluents
- 1 mm: rough waste water, inlet of municipal waste water

4 Background factor

This field allows to add partially the turbidity measurement made on another channel to improve
the COD accuracy, considering that particles may contains organic matter that cannot been
measured directly by UV absorption. This factor is experimentally determined according to
laboratory measurements.

The final COD value is:

COD= COD + background_factor x turbidity

No turbidity compensation is done if this factor stay at zero (default value).

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5 Linked channel number for turbidity compensation

This field displays the turbidity channel number if the turbidity compensation is applied.
This number doesn't matter if the turbidity factor remains at zero.
.

4.17.3 Test

The check screen displays the current value of the sample inside the flow cell.

Press on the play key to update the measurement.

2 3 4 5 6 7

1 Measured value

Immediate measurement obtained with the sample inside the flow cell. Can be renewed by
pressing on the play button.

2 Sampling pump button

This button run the sampling pump for 10 seconds to renew the sample.

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3 Reagent pump button

This button injects the cleaning solution. It can be used to check the zero.

4 Stop button

This button stop an engaged measuring cycle or a running pump.

5 Signal test button

This button display the lamp signal. The PH1 (peak photo detector) value should normally be
between 1000 and 2500 on pure water, PH2 (reference photodiode) should be between 1000
and 2500 also on pure water.

PH1 decrease according to the organic matter concentration and the turbidity of the sample.
PH2 is affected only by the turbidity of the sample. The screen is shown below.

6 Zero button

This button is used for zeroing the measurement.

Put first pure water inside the flow cell before pressing this button.

7 Start measurement

This button update the measured value directly with what is inside the flow cell.

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Signal screen:

4.17.4 Calibration

The calibration screen below enable to recalibrate the measurement channel.

See after this screen description the recommendations for recalibration.

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1 Last calibration window

This window displays the 10 last calibrations done with the date, time and the new calibration
factor.

2 Scan last calibrations

These two buttons allow to scroll up and down the 10 last calibration records.

3 Calibration factor

This field allows changing manually the calibration factor of the channel.
This factor is normally changed automatically while doing a calibration procedure by pressing on
the “ADJUST” button.

4 Offset

This field shows the internal offset applied for zeroing.

It is updated at each zeroing and has normally not to be changed.

5 Calibration procedure

This button recalibrate the analyser according to a COD laboratory measurement.

The analyser must be in COD mode.

Enter the measured value obtained on the sample given to the laboratory
Then enter the laboratory COD value.

A new calibration factor is determined and recorded on the calibration history.

6 Linearization curve

As the Beer-Lambert law is not linear for high absorbance values, a linearization curve is
entered to compensate automatically this non-linearity. This button display the linearization
curve and enable to enter or check the linearization values.

The Y-axis corresponds to the rough measurements entered on the M1 to M10 fields while the
X-axis corresponds to the standard or final measurement, entered on the S1 to S10 fields.

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The M1-M10 and S1-S10 values must be strictly increasing. It’s recommended to put 0.0 and
0.0 as starting values for S1 and M1. The final value is linearly extrapolated between these
points. Unused points at the end of the table must strictly remains at 0.0 both for S and M.

Recommendations for recalibration

The analyser gives the result in absorbance by meter by default. No calibration is required in
this measuring mode regarding the accuracy of flow cell optical path (+/- 0.1 mm).

If the measuring mode is in COD and after than the UV254-COD relation has been tested, the
analyser must be calibrated according to a COD laboratory measurement as each kind of
effluent has a specific UV254/COD ratio.

The default calibration in COD mode corresponds to river water with a calibration factor of 0.5.
The calibration factor for municipal wastewater is around 10.

Note that many saturated organic compounds like glucose or alcohol do not have UV
absorption.

To recalibrate the analyser in COD mode, proceed as follow:

- Take a representative sample and bring it to a laboratory for a COD measurement.

- Do a manual measurement on this sample using the check screen (It's recommended to check
first the zero).

- When the laboratory measurement is know, go on the calibration screen and press on the
“ADJUST” button of the check screen. Enter the measurement given by the analyser and then
enter the laboratory value on the keypad.

The calibration is finished. A new calibration factor has been calculated and has been recorded
inside the calibration history displayed on the check screen.

This new calibration factor will be taken in account for all the further measurements.

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4.17.5 Maintenance and troubleshooting

Maintenance

The maintenance is limited to refilling of the 5% H2SO4 cleaning solution.

Troubleshooting

Symptoms Origin

Negative value - Bad zero or auto-zero: the cleaning solution has been prepared with
polluted water or fault in the acid cleaning system (no flow, pump
failure)

Value is too low - No water in the flow cell (clogging, bad inlet connection, fault in the
peristaltic pump)

- Bad zero or auto-zero: cleaning solution has been prepared with

polluted water or fault in the acid cleaning system (no flow, pump
failure)

- Bad calibration: check or redo a calibration

Value is too high - Bad calibration: check or redo the calibration.

Unstable value - Deposit or dirty on the flow cell, check the light level on the check
screen

- Moving optical part (check manually after transportation).

- Bad calibration, check with a standard.

Measuring errors

Error Signification Origin / Remediation

no

1 No signal at all from - Peak photo detector PH1 disconnected or failure

the peak photo
detector PH1

2 No signal at all from - Reference photo detector PH2 disconnected or failure

the reference photo
detector PH2

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3 The light level is too - Check that there is no bubble inside the flow cell, if yes check
high on the peak the fittings
photo detector PH1
- Failure on the photo detector board or cable (repair or
replace)

4 The light level is too - Check that there is no bubble inside the flow cell, if yes check
high on the reference the fittings
photo detector PH2
- Failure on the photo detector board or cable (repair or
replace)

5 The light level is too - Over range or very high turbidity. Recheck on pure water
low on the peak
photo detector PH1 - Dirty on the flow cell windows. Check if the automatic
cleaning system is working properly

- Lamp failure or lamp supply board failure. Check if flashes

are visible during the measurement. Check the neon indicator
N1 on the XENON400 board.

6 The light level is too - Over range or very high turbidity. Recheck on pure water
low on the reference
photo detector PH2 - Dirty on the flow cell windows. Check if the automatic
cleaning system is working properly

- Lamp failure or lamp supply board failure. Check if flashes

are visible during the measurement. Check the neon indicator
N1 on the XENON400 board that must be always lighted.

4.17.6 Specifications

Specifications in UV254 mode

Flow cell 1 mm 3 mm 10 mm

Measuring range 2000 Abs/m 600 Abs/m 200 Abs/m

Accuracy +/- 2 Abs/m or +/- 5%* +/- 0.6 Abs/m or +/- 5%* +/- 0.2 Abs/m or +/- 5%*

* whichever is greater

Measuring time: 5 seconds

Note: the specifications is COD mode are specific to the effluent.

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5 General Maintenance and troubleshooting

The maintenance is limited to the replacement of the peristaltic pump tubing, to the refilling of
the cleaning or reagent solution (depending on the configuration) and to the cleaning of the
external probe if no automatic air cleaning is installed.

The period of replacement of the pump tubing depends on the use. A weekly checking is
recommended. The typical lifetime with Pharmed® tubing is estimated to about 1000 hours of
continuous operation.

General troubleshooting:

Disconnect the power cord before servicing!

Symptoms Checking / Origin

The screen remains totally black after - Check the power socket
connecting the power cord.
- Check J1 connector (mains input, high voltage!)
AND
- Check J2 connector on the DSP400TFT (mains
The red LED D11 on the DSP400TFT input for the power supply, high voltage!)
board is OFF.
- Check J3 connector (12V DC output from the
power supply)

- Failure on the power supply of the DSP400TFT

board

The screen remains totally black after

connecting the power cord.
- Check the backlight connector of the screen J44.
AND
- Failure off the DSP400TFT board.
The red LED D11 on the DSP400TFT
board is ON.

The screen is lighted but nothing is - Check the screen connector J6 on the top right of
displayed or unstable display. the DSP400TFT board

- Failure off the DSP400TFT board.

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6 General Specifications

Sample temperature: 0 ºC to 80 ºC

Sample pressure: 4 bar (4000 hPa or 60 psi) without sampling pump or 1 bar (1000 hPa or
15 psi) with the sampling pump

Wet materials: Polypropylene (PP), quartz, glass, PTFE, FKM (Viton)

Sample Inlet/outlet: Double ring fittings for semi-rigid plastic tubing

External diameter (OD) 9.6 mm (3/8”)
Internal diameter (ID) 6.4 mm (1/4”)

Reagent Inlet/outlet: Barbed fittings for soft plastic tubing

Internal diameter (ID) 3.2 to 4 mm

Cleaning solution: Recommended: 5% sulphuric acid (H2SO4)

(optional) (Up to 20% for special applications)
Typical consumption: 220 ml per day

Display: Colour TFT LCD, Size: 5.7”, resolution: 320 x 240 pixels
LED backlight
Resistive touch screen

Memory: 5000 records (up to 16 measurement channels) with date and time

Communication: - RS232 - MODBUS protocol or HTTP/HTML5

- RS485 - MODBUS protocol for probes

- Wi-Fi (IEEE 802.11b), optional, SMA connector with antenna

Distance: about 30 meters indoors
MODBUS protocol or HTTP/HTML5

- Ethernet (10/100BASE-T) optional, RJ45 connector

MODBUS protocol or HTTP/HTML5

USB port For USB memory keys, any format (FAT16, FAT32)
Standard USB connector type A with IP68 protective cap
- Recorded measurement downloads (compatible with Excel®)
- Complete configuration backup/restore (proprietary format)
- Screen copy in BMP format (compatible with Windows®)
- Software update

Extensions: 12 internal sockets for input modules (electrodes, 4-20 mA input),

output modules (4-20 mA) or relays modules

Power supply: 90-264 VAC / maxi 40 VA / 50-60Hz

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Operating limits: 0 to 50 ºC

Safety standard: IEC 61010-1, EN 61010-1

EMC standard: EN61326/A1/A2/A3, IEC61000-3-2, IEC61000-3-3, IEC61000-4-2,

IEC61000-4-3, IEC61000-4-4, IEC61000-4-5, IEC61000-4-6, IEC61000-4-11

Enclosure: IP65 / Nema 4X, steel with epoxy coating

Dimensions: 420 x 360 x 200 mm

Weight: 14 kg approx. (depending of the configuration)

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