LAMBDA OMNICOLL Fraction Collector and Sampler Manual

LAMBDA OMNICOLL
Fraction Collector - Sampler
OPERATION MANUAL
LAMBDA Laboratory Instruments LAMBDA CZ s.r.o.

Dr. Pavel Lehky Lozibky 1
Imfeldsteig 12 CZ-61400 Brno
CH-8037 Zurich Czech Republic
Switzerland Tel/Fax: +420 545578643
Tel/Fax: +41 (0)444502071/72 Hotline: +420 603274677
www.lambda-instruments.com
LAMBDA Laboratory Instruments, Imfeldsteig 12, CH-8037 Zurich, Switzerland, [email protected]
LAMBDA OMNICOLL fraction collector-sampler Operation Manual 1
LAMBDA OMNICOLL fraction collector – sampler
The LAMBDA OMNICOLL introduces new concepts in fraction collection and sampling. The
OMNICOLL fraction collector and sampler can be used for all kinds of chromatography. In
the number of simultaneous chromatographic streams the OMNICOLL is not outperformed
by any other fraction collector on the market.
• Collects fractions in any racks of your choice

• Collects unlimited number of fractions in single or multiple fractions
• No danger of spilling; the complete collector is placed above the tubes
• Unlimited number of programs
• Extremely easy programming of rack and tube position by means of a simple pen
• Fractionation according to time (0.1-999.9 min and 1-9999 min) or volume
(0.05-500 ml or 0.1-30 l) or drops (with optional drop-counter)
• Sampling with pause (0.1-9999 min) and line washing; the fraction collector can be
used for sampling during cell cultures, fermentations, chemical reactions, etc.
• Can be placed in a cold bath or any other thermo-stabilized container
• Solvent resistant metal construction with tubes easily accessible from all sides
• Multicolumn attachment for simultaneous fraction collection (optional)
• Low voltage power supply – battery operation possible for field-applications)
• RS-232 interface (optional)
LAMBDA Laboratory Instruments

is developer and producer of special laboratory instruments mainly for biotechnology,
microbiology, food and agricultural, chemical and pharmaceutical research and development
as well as for general laboratory and research applications.
LAMBDA MINIFOR - innovative and compact laboratory fermenter / bioreactor
LAMBDA OMNICOLL - fraction collector with new concept for unlimited number of fractions
LAMBDA PRECIFLOW, MULTIFLOW, HIFLOW and MAXIFLOW peristaltic pumps -

practical, precise and extremely compact
LAMBDA SAFETY POWDER DOSER - allows automatic addition of powder without spoon.
Safe operation with hazardous material (GLP)
LAMBDA VIT-FIT polyvalent syringe pump with extremely robust mechanics - programmable
infusion and filling from micro syringes to large volume syringes of 150 ml without adapter
LAMBDA MASSFLOW is precise gas flow measurement and control with recording option
LAMBDA PUMP-FLOW INTEGRATOR - with LAMBDA pumps and doser allows the
visualization and recording of the pumped volume
www.lambda-instruments.com [email protected] Hotline: +420 603 274 677

TABLE OF CONTENTS
1. SETTING UP THE FRACTION COLLECTOR................................................. 3
2. PROGRAMMING OF THE FRACTION COLLECTOR .................................... 4

2.1 Principle of programming..............................................................................................4
2.2 Use of the OMNICOLL fraction collector with the supplied tube racks .........................4
2.3 Programming the OMNICOLL for any tube rack or recipients ......................................5
2.4 Start of the fraction collection .......................................................................................8
2.5 Fraction collection according to volume........................................................................9
2.6 Calibration of the peristaltic pump and the OMNICOLL collector .................................9
2.7 How to increase the capacity of the OMNICOLL fraction collector .............................10
2.8 Fraction collection with a time interval between fractions (“high” mode) ....................11
2.9 Remote control of the OMNICOLL fraction collector and sampler..............................12
3. PRACTICAL ADVICE.................................................................................... 13
4. MAINTENANCE ............................................................................................ 14
5. FOR YOUR SAFETY ..................................................................................... 14
6. ACCESSORIES AND SPARE PARTS.......................................................... 14
7. TECHNICAL SPECIFICATIONS ................................................................... 16

7.1 General specifications ................................................................................................16
7.2 Inputs/Outputs ............................................................................................................17
8. GUARANTEE ................................................................................................ 17
9. APPENDIX..................................................................................................... 18
9.1 Assembly of the fraction collecting tubing...................................................................18
9.2 Mounting of the front multiple stream adapter ............................................................19
9.3 Connecting the drop-counter detector ........................................................................22
9.4 RS communication protocol for fraction collector LAMBDA OMNICOLL ....................24
9.5 How to set the fraction collector address....................................................................26
9.6 RS-connection scheme...............................................................................................26

OPERATING INSTRUCTIONS
1. SETTING UP THE FRACTION COLLECTOR
• Unpack all components carefully.
• Push the tub-sheet into the slots of the yellow

supports (Fig. 1 A). Insert the black frame into the
corresponding slots of the yellow supports (Fig. 1 B).
Pay attention to proceed at the same speed on both
sides otherwise it will come to blocking. The tub-sheet
should not slip out of the slots.
Fig. 1
• Proceed in the same way for the opposite side.
• Fix both frames in position with four bolts (indicated by

the arrows in Fig. 2) using the hexagonal key.
Fig. 2
• Place the fixing mat in the tub-sheet (Fig. 3 A). When

needed clean the mat from dust and dirt with a humid
cloth. The mat has to be dry again before use.
• The metal rod fixed to the right side frame can be

used for fixing of a chromatography column, pump
etc. Push the metal bar through both fixation nuts and
fasten them to the frame with screws (Fig. 3 B, C).
• If needed, place the pump support on the metal bar

Fig. 3
(Fig. 3 D)
• It is recommended to use PTFE tubing of external

diameter of about 1.5 to 1.8 mm. Push the tubing
through the metal tube guide so that a tubing tip of
about 5 mm comes out of it (Fig. 4 A). Fix the tubing in
the right position with the screw (Fig. 4 B). Fix the
tubing carrying holder (tubing pipe) with the screw
(Fig. 4 C) so that it is about 1 cm above the tubes in
which the fractions will be taken. (See also Appendix
9.1 for a more detailed view and description of the
tubing assembly.)
Fig. 4

2. PROGRAMMING OF THE FRACTION COLLECTOR
2.1 Principle of programming
Laboratory practice shows that programming of instruments equipped with microprocessors

is not easy and mistakes are easily made, especially when such instruments are used only
occasionally.
We have developed a new method, which should eliminate such problems and make the
programming easy for all types of tube stands.
The fraction positions are indicated by white lines

drawn on the black magnetic coding band (Fig. 5).
The white lines should be about 2 mm broad and
full-length. These lines are detected by the photo-
detectors of the OMNICOLL fraction collector and
the fraction collector stops at these positions. The
actual fraction position is about 1 mm after the first Fig. 5
edge of the white lines.
The beginning and the end of a row are indicated by short lines (about half the length of the
normal stripes or less and ~5 mm broad) made on the lower edge (for the beginning) and
upper edge (for the end) of the coding band, respectively (Fig. 5 A and C). These beginning
and end signals determine at the same time the position of the first and the last
fraction in the row. The fraction position is about 2 mm after the first edge of the white line
of the beginning and end stripes.
The distance between the fraction rows is programmed in a similar way (Fig. 5 B). If you wish
the collector to switch off automatically after the last row, draw the last line broader (approx.
1 cm).
This simple coding principle allows the use of any racks and fraction stands or other
recipients with the LAMBDA OMNICOLL fraction collector and sampler.
2.2 Use of the OMNICOLL fraction collector with the supplied tube racks
The coding bands with lines are ready for use with the
Fig. 6
supplied racks.
• Place the longer and narrower coding band (Y-axis) on

the left inner metal frame just under the gear-bar (Fig. 6).
The position of this magnetic band can be modified easily.
The first line should lie approx. 10 cm from the front of the
frame.
• Push the X-axis band holder out of the fraction collector

control unit (Fig. 7 D) and place the shorter and broader
coding band inside (Fig. 5 C). Replace the holder into the
control unit, with the white stripes facing up (Fig. 7 D).
Place the coding strip strictly horizontally into the

corresponding slot! Otherwise, the optical detectors
could be damaged. Fig. 7

2.3 Programming the OMNICOLL for any tube rack or recipients
2.3.1 Setting of the tube distance in the row (X-axis)
• Measure the distance between the tube centres of your rack and choose the number of
fractions in a row.
• Draw the number of lines with the supplied white pen in the determined fraction distance.
You could also simply place the coding band on the tubes and mark the fraction position
with a normal pencil. On the marked places you can then draw the white lines correctly.
Reserve the first approx. 2 cm of the band for the beginning and end-position signals.
Draw these beginning and end signals on the lower and upper edge of the band similarly
as in Fig. 5 A and C. Leave a minimum of 6 mm of free space between the first and last
line of the row and the beginning- and end-signals. These signals in the lower or upper
edge of the band tell the microprocessor that the arm of the control unit is on the right or
the left side of the unit.
• Put the coding band into the holder and insert it into the control unit, with the white stripes
facing up (Fig. 7 D).
2.3.2 Setting of the row distance (Y-axis)
• Measure the distance between rows. When several tube racks are used pay attention to
different distance between the last row of the first rack and the first row of the second
rack.
• Mark the row distances with a pencil on the coding band for Y-axis and draw white lines
with the correction pen (Fig. 5 B). If you wish to stop the fraction collector after the last
row, make the last line broader (approx. 1 cm). This functions as the stop signal.
• Place the band on the left internal part of the metal frame just under the gear bar (Fig.6).
Since the coding band is kept in place by magnetic force it can be easily positioned
according your needs. The first row line should be ca 10 cm from the front. (When the
control unit is placed in the starting position on the front of the frame, the photoelectric
detector on the left side of the control unit must be situated before the first line. Otherwise
the control unit will stop at the position of the next row.)

2.3.3 The OMNICOLL fraction collector control panel
2.3.4 Selection of linear, meander-like or row collection
The LAMBDA OMNICOLL fraction collector-sampler allows the following operation modes:
Line: The fractions are collected from the left to the right. After the last fraction of
the row, the collecting arm moves to the left to the first position of the next
row.
Meander: The fractions are taken alternatively from the left to the right and in the
following row from the right to the left and so forth.
Row: The collecting arm does not move within the row. The collector moves only
from row to row. This collection mode is mainly used in the simultaneous
(multi-channel) fraction collection mode.
You can select the linear, meander or row collection in the following way:
• Switch off the plug-in power supply by removing it from the mains. Press continuously the
button LINE while plugging the power supply into the mains again. “Line”, “Mean” or
“rouu” will appear on the display. By pressing the LINE button select the desired
collection way. Confirm your choice by pressing button SET.
• In the row mode, the position of the fraction collecting arm can be adjusted by using the
STEP button.
A small difference in the fraction positions with respect to the tube centers may
occur between fractions taken in opposite collection directions. If the fraction lines
are not too broad (about 2 mm), this difference in position is of approx. 1 mm.

2.3.5 Time resolution (0.1 or 1 minute)
The time resolution of the OMNICOLL fraction collector can be selected in the following way:
Press the button COUNT/TIME while connecting the power supply to the mains. The actual
time resolution will appear on the display “0.1M” or “1M”. By pressing the button
COUNT/TIME below the COUNT and TIME LEDs you can select to use 0.1 minute steps
“0.1M” or 1 minute steps “1M”. Confirm your choice by pressing the button SET.
2.3.6 Drops and count divider (division coefficient)
This function allows increasing considerably (by 60 times) the volume of fractions either
using a drop counter (art. no. 6926) or motor impulsions counter (counts). The divider can be
set either to divide by one or divide by 60. In the first setting every drop and every impulsion
of motor are counted. In the second setting sixty drops or impulsions give just one signal.
See Appendix 9.3 for the drop-counter detector connecting instructions.
• Switch off the plug-in power supply by removing it from the mains. Press the button
START while plugging the power supply into the mains again. “div” and then “=01” or
“=60” will appear on the display. By pressing the COUNT/TIME button select the desired
division factor. Confirm your choice by pressing the button SET.
• The volume of fractions can be also varied by the selection of tubing of internal diameters
from 0.5 to 4 mm, when constant volume fractions are collected by counting pump
impulsions (in “COUN” operation mode – COUNT LED is on, see section 2.5).
2.3.7 Address setting (PC control)
When the OMNICOLL fraction collector and sampler has been equipped with the optional
RS-232 interface, it can be controlled digitally, e.g. from a PC.
Disconnect the fraction collector from the mains. While keeping the direction button STEP
pressed connect the fraction collector to the mains again. The message “A” and two
numbers will appear on the display. This number from 00 to 99 is the current address of the
fraction collector. To change the address press the buttons Λ Λ Λ Λ under the display until
the desired number is obtained. Confirm your choice by pressing the button SET.
2.3.8 Service tests
The LAMBDA OMNICOLL fraction collector and sampler contains a series of tests, which
can help to determine a possible problem over distance by our technicians in collaboration
with our client. These tests are not to be used by our clients alone.
• Switch off the plug-in power supply by removing it from the mains. Press the button STOP
while plugging the power supply into the mains again. “0000” will appear on the display.
• A password will be given to you by our technician to enter the tests. Confirm your
selection by pressing the button SET.

2.4 Start of the fraction collection
• Connect the cable of the control unit of the LAMBDA OMNICOLL fraction collector and
sampler with the cable of the power supply plug.
• Place the control unit just at the beginning of the gear rails (Fig. 4 and 7 A). The distance
from the edge should be the same on the left and right sides. The gear wheels of the
control unit must engage correctly in the gears of both rails. Be sure that nothing will
disturb the movement of the control unit during fraction collection.
• Connect the power supply to the AC mains (95-240 V/50-60 Hz). The control unit and its
arm move automatically to the first position of the first row.
• Press the SET button (a short acoustic signal is heard). By pressing the COUNT/TIME
button you can select fractionation according to time or volume. The yellow LED indicates
which collection mode has been selected (see also section 0).
• Use the four buttons Λ Λ Λ Λ under the display to select the desired value. Confirm it by
pressing the SET button again (a long acoustical signal is heard). The fraction time can be
set from 0.1 min to 999.9 min (about 16.6 hours) in 0.1 min steps or from 1 to 9999 min in
1 min steps. To change the time resolution, please refer to section 2.3.5. For the setting of
the volume, please refer to section 2.5.
• Put the dispensing tubing through the tubing holder as describe in appendix 9.1 and
tighten the tube holder in the collector arm so that the dispensing tubing is about 1 cm
above the tubes or recipients.
• Place the tube rack on the fixing mat, so that the first tube is just under the tip of the
tubing and the edge of the rack is parallel to the yellow frontal support (Fig. 7 B). Place the
additional tube racks immediately behind the first and following racks.
• Make sure that nothing will block the movement of the control unit during the fraction
collection and that the tubing length is sufficient for taking all fractions or samples.
• Press the START button to begin the collection of fractions or sample taking. The green
LED will be on and, if you use a LAMBDA peristaltic pump (PRECIFLOW, MULTIFLOW,
HIFLOW or MAXIFLOW), it will be activated automatically.
• You may use the magnetic block to stop the fraction collection. Place the supplied magnet
on the right inner side of the frame (Fig. 7 C).
It may happen that the position of the optical sensors lies outside the coding marks
of the coding strip inserted into the carriage. When started in such a position, the
fraction collecting arm will move either to the extreme left or right position according
to the instrument setting and is blocked there. After several seconds an automatic
protection will switch off the motor. To move the arm into the coding mark signals
just press the button STEP. The arm will move to the border coding line and will
afterward operate correctly within the marked limits of the inserted coding stripe.
If the moving arm is blocked in its movement or is operated without inserted coding
strip, the motor will stop and the message “Eror” will appear on the display. Switch
off the power, resolve the problem and start again as usual.

2.5 Fraction collection according to volume
If you use the OMNICOLL fraction collector together with LAMBDA peristaltic pumps
PRECIFLOW, MULTIFLOW, HIFLOW or MAXIFLOW, you can take fractions of precise
volume (from 0.05 to 500 ml or 0.6 to 30 liters per fraction). The heart of these pumps is a
stepping motor or BLDC motor, controlled by a generator of electric impulses
(microprocessor). After each impulse the pump motor moves by one step. This movement
displaces a very small and precise volume of liquid. The collector counts these impulses and
thus makes it possible to deliver an exact volume of liquid for each fraction. This method is
an attractive alternative to the old drop counting procedure, where the volume is affected by
the viscosity, surface tension etc. of the respective liquid.
Since the diameter of the tubing used in the peristaltic pumps affects the speed of the flow
(flow rate), the pump has to be calibrated before use in order to establish the relation
between the count number and the delivered liquid volume (see section 2.6).
2.6 Calibration of the peristaltic pump and the OMNICOLL collector
• The LAMBDA peristaltic pump is connected to the OMNICOLL fraction collector via the
remote control cable (Art. No. 4810-s) and the communication module box (Art. No. 6911
or Art. No. 6929). The plug-in power supply is also connected to this connection box. (It is
impossible to make a wrong connection because the connectors will not fit to the wrong
sockets).
• With the COUNT/TIME button select the count modus (the COUNT LED will be on).
• Press the SET button and select a certain count number with the buttons Λ Λ Λ Λ, for
example 100. Confirm it by pressing the SET button again.
• Select the speed control on the peristaltic pump (e.g. 999), choose the pump rotation
direction and switch the pump on.
• Press the START button on the collector to start fraction collection. If the tubing was not
filled completely with the liquid, for example water, take a second fraction.
• Measure the volume of this fraction. This volume corresponds to the preset count number.
• From this ratio (volume/count number) you can easily calculate the count number
corresponding to the desired fraction volume.
Since tubing of 0.5 to 4 mm of internal diameter (with a tubing wall thickness ~1 mm)
can be used in the LAMBDA peristaltic pumps, the fraction volume can be selected in
a wide range. For example, when tubing with an internal diameter of 3 mm is used,
then one count corresponds approximately to one drop.

2.7 How to increase the capacity of the OMNICOLL fraction collector
Since several lower parts of the collector can be coupled together, the capacity of the
collector can be increased many times. The control unit can move freely from one lower unit
to the other on the gear rails. Only two rectangular coupling bars are needed to keep two
units together.
• Remove the two blind bars from the rear side of the frame of the first lower part unit and
the front side of the second lower part unit. Insert the coupling bars (Art. No. 6912) on
their place and fix with the four bolts.
• Be aware that you must remove the fixed stop signal from the first frame unit and place it
onto the next one. You may also use the magnetic stop contact.
• Make sure that nothing will block the movement of the control unit during the fraction
collection.
The following pictures show how the tube capacity of the LAMBDA OMNICOLL fraction
collector and sampler can easily be increased. Virtually any number of capacity extensions
can be added.

2.8 Fraction collection with a time interval between fractions (“high” mode)
You can also use the LAMBDA OMNICOLL fraction collector and sampler for the collection
of samples with a time interval between consecutive fractions varying between 0.1 min and
16.6 hours (999.9 min) or 1 min and 166 hours (9999 min). This is particularly welcome
during fermentations and other biological and chemical processes. For this application you
have to switch the microprocessor of the control unit to the “high” mode.
Programming in the high mode:
• Pull out the plug-in power supply from the mains (the voltage on the microprocessor has
to be 0 V).
• Press the SET button on the control unit and connect the plug-in power supply again to
the mains.
• Use the button COUNT/TIME to select the “high” modus. Confirm the selection by
pressing the SET button (long beep signal).
• In the “high“ modus you can successively select the parameters TIME, COUNT, PAUSE
and NUMBER.
The programming of these parameters is the following:
After selection of the parameter with the COUNT/TIME button, press the SET button. After a
short beep the name of the parameter will appear during one second, followed by last used
value of the parameter. You can change this value by means of the four buttons Λ Λ Λ Λ
under the display. Press the button SET to confirm the new value (long beep). The
programmed parameter can be recognized also by means of the state of the LEDs,
according to the following table:
PARAMETER TIME LED COUNT LED DISPLAY

TIME on off tiMe
COUNT off on coUn
PAUSE on on PAUS
NUMBER off off nuMb
After setting of the parameters, the fraction collection is started by the START button. The
collector will take the number of samples as programmed under the NUMBER setting and
will stop for the time programmed under the PAUSE setting. This cycle will go on until a
STOP signal will be encountered. The LAMBDA peristaltic pump is activated automatically
only during fraction collection.

2.9 Remote control of the OMNICOLL fraction collector and sampler
It can sometimes useful to take samples during a process at a moment, which is not known
in advance. For example, when during fermentation (or any other biological, chemical or
physical processes) certain limit values have been attained and an alarm signal is activated.
This signal can be used to take a fraction. This fraction may be essential to recognize why
such an alarm has happened.
The fraction collector can take one or several samples after detection of a signal 3 to 12 V (or
12 to 30V with a 3300 ohm resistor switched in series). The LAMBDA peristaltic pump will be
activated automatically.
For safety reasons the voltage of the external signal must not exceed 48 V to earth!
2.9.1 Collection of single samples
If the collector operates in normal modus, it will take a single fraction of selected time or
volume after the reception of a signal impulsion. It will then move to the next position and
wait the following signal. This operation will go on until the detection of a stop signal.
2.9.2 Collection of multiple samples
If the collector operates in the high modus, after detection of a signal it will take the number
of fractions programmed under “NUMB”, e.g. from 1 to 999 (see section 2.8).
This feature is particularly interesting when it is necessary to wash tubing before taking the
fresh significant sample. This is very important during fermentation processes or cell
cultures, where the culture may be inactivated when kept in the tubing during long periods of
time. The same is true for any other process where the sample quality may be affected by
staying in the tubing.

3. PRACTICAL ADVICE
• Keep all position signal lines clean. If necessary you can remove old or damaged lines
with acetone and draw new lines at the same position. Instead of using a white pen you
may also use strips of white adhesive tape (~1.5 to 2 mm wide).
• Keep the fixing mat clean and dry. Humidity or dust will provide only low adhesion of the
fixing mat. The fixing mat can be washed with water to regain the original adhesion power.
• Take only 10 fractions in a row, even though your stand has more positions. It will help
you to find the desired fraction more easily and you will eliminate mistakes.
• The alignment of the tube rack is easier if you press it against the front part of the frame
supports. By pushing the magnetic Y-axis coding strip you will find the position
corresponding to this rack position.
• By removing the tub sheet from the frame unit it is possible to use even larger or taller
collecting containers. We can also supply tub sheets for use with funnels according to
your needs. This makes it possible to use even largest containers for taking fractions. For
such applications a modified tubing holder can also be made.
• On demand we can also supply an adapter for simultaneous collection of multiple

fractions (see below). This enables to collect simultaneously the streams of several
chromatographic columns. (See Appendix 9.2 for multi-stream operation instructions.)
Multiple fraction collection – multi stream Simultaneous fraction collection for more than 18
assembly (mobile front version, art. no. simultaneous fractions (fixed rear version, art. no.
6920) 6923)

4. MAINTENANCE
The LAMBDA OMNICOLL fraction collector does not require any special maintenance.
Keep you collector clean. If necessary clean it with damp cloth. You may use neutral
detergents or ethyl alcohol.
If you have any difficulties or questions concerning your OMNICOLL fraction collector, please
contact our service office.
5. FOR YOUR SAFETY

Thanks to the use of a plug-in power supply giving only a low voltage of 9 V DC the danger
of electrical shock during the use of the LAMBDA OMNICOLL fraction collector and sampler
has been virtually eliminated.
If the fraction collector is not used for an extended period of time, disconnect it from the
mains. A modern miniaturized switching power supply is used, which has only a negligible
consumption of electric current when the fraction collector is not in use.
6. ACCESSORIES AND SPARE PARTS

Art. No. Accessories
4801 PRECIFLOW peristaltic pump, 0-600 ml/hr, not programmable
4901 MULTIFLOW peristaltic pump, 0-600 ml/hr, programmable
5001 HIFLOW peristaltic pump, 0-3’000 ml/hr, programmable
6001 MAXIFLOW peristaltic pump, 0-10’000 ml/hr, programmable
6910 Remote control cable for fraction collector (analogue)
6910-rs RS-232 connection cable
6911 Communication module for pump switching and RS-232 connection
6912 Set for keeping two lower parts together
6913-1 Tube rack for tubes diam. 12/13 mm
6913-2 Tube rack for tubes diam. 16 mm
6920 Accessory for “moving” front side simultaneous fractions (incl. 3 pipe guides)
6923 Accessory for “fixed” rear side multiple fractions (up to more than 18 fractions)
6930 Gear bar extensions (2 pieces)
6926 Drop-counter (detector)
6927 Inert valve (one way)
6929 Communication module with RS-232, drop counting electronics, valve interface
6914 Pump remote control cable (2 poles, with open end)
4810-s Remote control cable for LAMBDA pumps (5 poles)
Spare parts
6902 Support (lower part of collector)
6903 Plug-in power supply (9V)
6904 Fixing mat
6905 Support for pump

6906-S Supporting rod for columns diam. 12 mm 60cm

6906-L Supporting rod for columns diam. 12 mm 100cm
6907 Lateral supports for rod
6908 Tube guide
6909 Stop magnet
6916 PTFE tubing ext. diam. 1.8 mm (5 m)
6917 Coding magnetic band for X-axis (5 pces)
6918 Coding magnetic band for Y-axis (5 pces)
6919 Support for coding magnetic band for X-axis
6921 Pipe guide for simultaneous fractions 1 pce
6922 Magnetic tubing guide
6924 Hexagonal key set
6925 O-ring for tubing fixing (5 pces)
6928 White pen for fraction position marking

7. TECHNICAL SPECIFICATIONS
7.1 General specifications
Type: LAMBDA OMNICOLL – microprocessor-controlled

programmable fraction collector – sampler
Collection mode: Linear (line), meander (zigzag) or row collection
Normal modus:
Time: 0.1 to 999.9 minutes (16.67 hours) in 0.1 min steps or
1 to 9999 minutes (166.7 hours) in 1 min steps
Volume: 0.01 to 500 ml or 0.6 to 30 liters (external counts using
LAMBDA peristaltic pump)
High modus: same as Normal modus, but with a pause between fractions
(from 0.1 to 999.9 minutes or 1 to 9999 minutes)
Remote control:
Normal modus: Collector takes a single fraction after an external voltage
pulse of 3-12 V (or 12-30 V with a 3300 ohm resistor)
High modus: Collector takes a 1 to 999 fractions after a single external
voltage pulse of 3-12 V (or 12-30 V with a 3300 ohm resistor)
Capacity: Any tube rack or container type with a surface smaller than
45x31cm
using supplied racks: 360 tubes of 12-13 mm diameter
240 tubes of 16 mm diameter
The capacity can be increased many times by coupling
several lower fraction collector parts together.
Non-volatile memory: Storage of all settings
Interface: RS-232 (optional)
Power supply: 95–240 V/50–60 Hz AC plug-in power supply with DC 9V/12W
output; possible field operation on 12 V accumulator
Dimensions: 34 (W) x 30 (H) x 49 (D) cm
Weight: 6.5 kg
Safety: CE, meets IEC 1010/1 norm for laboratory instruments
Operation temperature: 0-40 °C
Operation humidity: 0-90% RH, non-condensing
Remote control: 0-10 V; (option 0-20 or 4-20 mA)
Fuse: 1.5 A (on printed circuit board)
For safety reasons the voltage of the external signal must not exceed 48 V to earth!

7.2 Inputs/Outputs
No. Color Description

1 blue Input remote control +3-12 V
2 green Impulses of the stepping motor of the LAMBDA
peristaltic pump (0 and 12 V)
3 white Reserved for RS-232 TTTL
4 red Input voltage + 9 V
5 brown Output remote control for the pump (+ 9-12 V)
6 yellow Reserved for RS-232 RTTL shield =common zero
7 Shielding is the common ground
6-pole connector
8. GUARANTEE
LAMBDA provides a two-year guarantee on material and manufacturing defects, if the
instrument was used according to the operation manual.
Conditions of guarantee:
The instrument must be returned with a complete description of the defect or problem. In
order to send back the equipment for repair, you will need a returns authorization number
from LAMBDA.
The customer will send the instrument to our service office.
Damage or loss of items during transport will not be compensated for by LAMBDA.
Failure to fulfill these requirements will disqualify the customer from compensation.
Serial Number:
Guarantee from:
LAMBDA Laboratory Instruments LAMBDA CZ s.r.o.

Dr. Pavel Lehky Lozibky 1
Imfeldsteig 12 CZ-61400 Brno
CH-8037 Zurich, Switzerland Czech Republic
Tel/Fax: +41 444502071/72 Tel/Fax: +420 545578643
[email protected] Hotline: +420 603274677
www.lambda-instruments.com www.fractioncollector.info

9. APPENDIX
9.1 Assembly of the fraction collecting tubing
Assembly of the fraction collecting tubing:
(a) PTFE-tubing (ext. diameter 1.8 mm)

(b) Fixing nut
(c) O-ring (1 x 1.5 mm)
(d) Tubing guide
Push the delivered PTFE tubing (a) through

the nut (b).
Slide the o-ring (c) on the PTFE tubing (a).
Push the tubing (a) through the tubing guide

(d) so that few mm (~5 mm) of PTFE tubing
reach out of the tubing guide. This is
important so that drops form only at the tip
of PTFE tubing.
Screw the nut (b) onto the tubing guide (d)

to such an extent that the PTFE tubing does
not move freely inside the tubing guide. Do
not screw more strongly than necessary!
Fix the tubing guide (d) by the screw of the

holder (e) on the front of the moving arm of
the fraction collector at a convenient
distance above the tubes.
Let the PTFE tubing pass through the

magnetic tubing holder (f). This magnetic
tubing holder is conveniently placed in the
center of the fraction collector carriage and
control unit.

9.2 Mounting of the front multiple stream adapter
When using the front multiple stream adapter, the pump support plate with the fixing
rod must be removed.
Unscrew the tubing guide holder screw on

the moving arm (a) of the OMNICOLL
fraction collector.
Insert the rectangular bar (b) into the bar

holder (c).
Slide the right end of the bar (b) through the

opening on the guide plate (d).
Note: Please remove the pump support

plate and the fixing rod when using the front
multiple stream adapter.
Screw the bar holder (c) on the moving arm

of the fraction collector with the
corresponding socket key (hex-key), see (a)
above. Use e.g. a thin spatula to push the
screw through the moving arm.

Slide the tubing guide (e) into the tubing

guide holder (f), so that a convenient height
above the tubes is obtained.
Fix the tubing guide (e) by the worm screw

on the side of tubing guide holder (f) using
the corresponding socket key (hex-key) (g).
Place the tubing guide holder (f) at the

desired fraction position and secure it with
the socket head screw using the
corresponding socket key (Allen key) (h).
Place the tubing guide holder (f) in position

above the first fraction of each stream
(channel). If 3 streams of 4 fractions are
taken in the row, place the first tubing guide
over tube no. 1 (i), the second tubing guide
over tube no. 5 (j) and the third tubing guide
over tube no. 9 (k).
Prepare the position coding strip for

fractions. For 3 streams distributed in 3 x 4
fractions, the coding strip will have two end
marks (start and stop signal, corresponding
to fraction #1 and fraction #4, in this case)
and two fraction lines in between (fraction
#2 and fraction #3). The position of the
fraction collection is indicated by the arrows.
Place the coding strip strictly horizontally

into the corresponding slot! Otherwise, the
optical detectors could be damaged.
- Place the carriage of the OMNICOLL fraction collector on the beginning of gear bars.
Take care, that the distance from the edge is the same on left and right side.
- Switch the power on. The carriage and the moving arm will stop at the position
corresponding to the first fraction.
- If necessary, correct this position by changing the position of the coding strip inside the
instrument, the position of the rectangular multi-fraction assembly bar or the position of
the rack.
- You can also adjust the position of the row coding stripe on the side of the lower part of
the fraction collector.
- When the position is correct, tighten the rectangular bar (b) to the bar holder (c) with the
screw.

It may happen that the position of the optical sensors lies outside the coding marks
of the coding strip inserted into the carriage. When started in such a position, the
fraction collecting arm will move either to the extreme left or right position according
to the instrument setting and is blocked there. After several seconds an automatic
protection will switch off the motor. To move the arm into the coding mark signals
just press the button STEP. The arm will move to the border coding line and will
afterward operate correctly within the marked limits of the inserted coding stripe.
If the moving arm is blocked in its movement or is operated without inserted coding
strip, the motor will stop and the message “Eror” will appear on the display. Switch
off the power, resolve the problem and start again as usual.
This multi-stream assembly is technically very simple and polyvalent. It allows an easy
adaptation of various experimental needs directly by the user.
Here, an example of taking 4x2 fractions in a

4-channel (4 streams) configuration is
shown.
Here, an example of taking 6x2 fractions in a

6-channel (6 streams) configuration is
shown.

9.3 Connecting the drop-counter detector
Unscrew partially the tubing guide holder

screw (a), so that the tubing guide (b) can
be rotated. Loosen partially the nut (c), so
that the tubing (d) can turn freely in the
tubing guide (b).
Screw the drop-counter detector (e) on the

lower part of the tubing guide (b).
Tighten the tubing guide (b) again by the

tubing guide holder screw (a) until the
tubing guide (b) does not move freely.
Tighten the nut (c), to prevent the free
movement of the tubing (d) in the tubing
guide (b).
Fix the drop counter cable (f) to the tubing

guide (b). This can be done e.g. by a Teflon
or similar tape or by a spiral wrap, an o-ring
(g) and the like.
Let the drop-counter cable (f) pass together

with the tubing (d) through the magnetic
tubing holder spring (h), which is optimally
placed in the center of the fraction collector
carriage.

The fixing of the drop-counter cable (f) to

the tubing guide (b) and also to the tubing
can also be done by a spiral wrap (i).
Connect the drop-counter (detector) cable

to the “DROP” socket of the communication
module (art. no. 6929).
Connect the OMNICOLL fraction collector to

the “OMNICOLL” socket and, if required,
the LAMBDA pump or valve to the
“PUMP/VALVE” socket.
Connect the power supply to the “POWER”

socket.
For remote control of the OMNICOLL

fraction collector and sampler the
“REMOTE” socket is used.

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LAMBDA OMNICOLL

Fraction Collector - Sampler

LAMBDA Laboratory Instruments LAMBDA CZ s.r.o.

LAMBDA OMNICOLL fraction collector – sampler

• Collects fractions in any racks of your choice

LAMBDA Laboratory Instruments

LAMBDA MINIFOR - innovative and compact laboratory fermenter / bioreactor

LAMBDA PRECIFLOW, MULTIFLOW, HIFLOW and MAXIFLOW peristaltic pumps -

www.lambda-instruments.com [email protected] Hotline: +420 603 274 677

1. SETTING UP THE FRACTION COLLECTOR................................................. 3

2. PROGRAMMING OF THE FRACTION COLLECTOR .................................... 4

5. FOR YOUR SAFETY ..................................................................................... 14

6. ACCESSORIES AND SPARE PARTS.......................................................... 14

7. TECHNICAL SPECIFICATIONS ................................................................... 16

www.lambda-instruments.com [email protected] Hotline: +420 603 274 677

1. SETTING UP THE FRACTION COLLECTOR

• Unpack all components carefully.

• Push the tub-sheet into the slots of the yellow

• Fix both frames in position with four bolts (indicated by

• Place the fixing mat in the tub-sheet (Fig. 3 A). When

• The metal rod fixed to the right side frame can be

• If needed, place the pump support on the metal bar

• It is recommended to use PTFE tubing of external

www.lambda-instruments.com [email protected] Hotline: +420 603 274 677

2. PROGRAMMING OF THE FRACTION COLLECTOR

2.1 Principle of programming

Laboratory practice shows that programming of instruments equipped with microprocessors

The fraction positions are indicated by white lines

• Place the longer and narrower coding band (Y-axis) on

• Push the X-axis band holder out of the fraction collector

Place the coding strip strictly horizontally into the

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2.3 Programming the OMNICOLL for any tube rack or recipients

2.3.1 Setting of the tube distance in the row (X-axis)

2.3.2 Setting of the row distance (Y-axis)

www.lambda-instruments.com [email protected] Hotline: +420 603 274 677

2.3.3 The OMNICOLL fraction collector control panel

2.3.4 Selection of linear, meander-like or row collection

www.lambda-instruments.com [email protected] Hotline: +420 603 274 677

2.3.5 Time resolution (0.1 or 1 minute)

2.3.6 Drops and count divider (division coefficient)

2.3.7 Address setting (PC control)

2.3.8 Service tests

www.lambda-instruments.com [email protected] Hotline: +420 603 274 677

2.4 Start of the fraction collection

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2.5 Fraction collection according to volume

2.6 Calibration of the peristaltic pump and the OMNICOLL collector

www.lambda-instruments.com [email protected] Hotline: +420 603 274 677

2.7 How to increase the capacity of the OMNICOLL fraction collector

www.lambda-instruments.com [email protected] Hotline: +420 603 274 677

Programming in the high mode:

The programming of these parameters is the following:

PARAMETER TIME LED COUNT LED DISPLAY

www.lambda-instruments.com [email protected] Hotline: +420 603 274 677

2.9 Remote control of the OMNICOLL fraction collector and sampler

2.9.1 Collection of single samples

2.9.2 Collection of multiple samples

www.lambda-instruments.com [email protected] Hotline: +420 603 274 677