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Microbiology Practicals Semester 5 & 6

The document outlines the practical components of several courses in microbiology and clinical diagnostics. It lists identification and counting of blood cells, isolation of normal flora, case studies, enzyme kinetics assays, growth curve analysis, mutagenesis techniques, screening of microorganisms, fermentation processes, separation methods, bioinformatics tools, antibiotic susceptibility testing, clinical diagnostics including blood tests, urine analysis, and thalassemia screening.

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vivek pattani
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1K views6 pages

Microbiology Practicals Semester 5 & 6

The document outlines the practical components of several courses in microbiology and clinical diagnostics. It lists identification and counting of blood cells, isolation of normal flora, case studies, enzyme kinetics assays, growth curve analysis, mutagenesis techniques, screening of microorganisms, fermentation processes, separation methods, bioinformatics tools, antibiotic susceptibility testing, clinical diagnostics including blood tests, urine analysis, and thalassemia screening.

Uploaded by

vivek pattani
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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MB-501- : Immunology and Clinical Microbiology

(PRACTICAL)
1. Identification of blood cells through microscopy
2. Total count of RBC
3. Total count of WBC
4. Differential count of WBC
5. Isolation of normal flora of skin
6. Isolation of normal flora of mouth
7. Understanding of the medical problems (Case Study)

REFERENCE BOOKS
1 Medical Laboratory Technology – Vol – I , II , III – Mukherji
K.L.
2 A Text Book in Medical Laboratory Technology – P.B.Godkar
3 Medical Bacteriology, Mycology and AIDS – N.C. Dey, T.K.
Dey, D.Sinha

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MB-502 : Prokaryotic Metabolism
(PRACTICAL)
1. Determination of Vmax and Km for amylase enzyme by performing substrate curve
with line weaver Burk plot.
2. Determination of Vmax and Km for phosphatase by performing substrate curve
with line weaver Burk plot.
3. Effect of temperature on amylase activity
4. Effect of pH on amylase activity
5. Study of Diauxic growth curve in E. coli
6. Determination of amino acid decarboxylase activity
7. Preparation of winogradsky column (Demonstration)

REFERENCE BOOKS
1 International student edition: Microbiology- A laboratory Manual 4th
edition. By James G. Chappuccino & Natalie Sherman
2 Bacteriological Techniques By F.J. Baker
3 Introduction to Microbial Techniques By Gunasekaran

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MB-503: Molecular Biology and Bio-engineering
(PRACTICAL)
1. Isolation of DNA ( only demonstration experiment)
2. Estimation of DNA
3. Conjugation in E. coli by plate method
4. Isolation of plasmid (Only demonstration experiment)
5. Transformation of plasmid
6. Isolation of RNA (only demonstration experiment )
7. Estimation of RNA
8. Isolation of Lactose non fermenter mutant of E. coli by physical mutagenesis
9. Isolation of antibiotic resistant bacterial population by gradient-plate method.
10. Isolation of streptomycin resistant mutants by Replica plating technique.
11. The Ames test: For detecting potential carcinogen (only demonstration experiment)

REFERENCE BOOKS
1 Biotechnology – Trevan M.D.
2 Advanced Molecular Biology – Twyman R.M.
3 Microbiology – Atlas R.M.
4 Microbiology – Prescott L.M.
5 Microbial Genetics – Freifilder. D
6. Principles of Gene Manipulation – Old and Primrose

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MB-601: Bioprocess Technology
(PRACTICAL)
1 Primary screening of industrially important microorganisms capable of producing:
Antibiotics, Organic acids, amylases
2 Bioassay of penicillin using Bacillus. spp.
3 Laboratory fermentation & estimation of Ethyl Alcohol by Saccharomyces
4 Laboratory fermentation & estimation of amylase by Bacillus spp.
5 Sterility testing of fermentation products (Demo)
6 Immobilization of yeast cells by Ca- alginate entrapment method & Determination
of viability of immobilized cells by invertase activity

REFERENCE BOOKS
1 International student edition: Microbiology- A laboratory Manual 4th edition. By
James G. Chappuccino & Natalie Sherman
2 Bacteriological Techniques By F.J. Baker
3 Introduction to Microbial Techniques By Gunasekaran

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MB-602: Analytical Techniques and Bioinformatics
(PRACTICAL)

1. Determination of absorbance maxima of KMnO4


2. Separation of carbohydrates by ascending paper chromatography
3. Separation of amino acids by circular paper chromatography
4. Separation of amino acids by Thin layer chromatography
5. Separation of nucleic acid by agarose gel electrophoresis
6. Separation of proteins by SDS PAGE (Demonstration)
7. Retrieval of 16S r DNA sequence of bacterial species from NCBI
8. Demonstration of BLAST analysis

REFERENCE BOOKS
1. Patel. R.J., Patel. K.R., Experimental Microbiology, Vol-I, Aditya Publications,
Ahmedabad, India
2. Patel. R.J., Patel. K.R., Experimental Microbiology, Vol-II, Aditya Publications,
Ahmedabad, India
3. Dubey. R.C., Maheshwari. D.K., Practical Microbiology, S.Chand & Company
Ltd., New Delhi
4. Konika Sharma, Manual of Microbiology – Tools and Techniques , Ane Books,
Delhi

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MB-603: Clinical Diagnostic Microbiology
(PRACTICAL)
1. Antibiotic susceptibility of the pathogens isolated from the clinical specimen
2. Study of Agglutination by
a Blood grouping
b Serodiagnosis of enteric fever by Widal test
c Serodiagnosis of syphilis by RPR Test
3. Haemoglobin estimation by Drabkin’s method
4. Bleeding time by filter paper technique and clotting time by capillary method
5. Erythrocyte Sedimentation Rate (ESR-demonstration)
6. Blood sugar estimation by GOD / POD method
7. Determination of Serum bilirubin
8. Determination of Serum Cholesterol
9. Physical, chemical and microscopic analysis of urine
10.Screening of Thalassemia by NESTROFT
11.Total count of platelets

REFERENCE BOOKS
1 A Handbook of Practical and Clinical Immunology – G.P.Talwar and S. K. Gupta –
CBS Publication
2 Medical Laboratory Technology – Vol – I , II , III – Mukherji K.L.
3 A Text Book in Medical Laboratory Technology – P.B.Godkar
4 Medical Bacteriology, Mycology and AIDS – N.C. Dey, T.K. Dey, D.Sinha

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