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    Report (Chemical Kinematics) : Name: Muhammed Aadil Khan (M.A.K) Reg No: 21416042 Group: A Date: Aim

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    This document is a report on an experiment to calculate the specific rotation of sucrose using a polarimeter and to observe the cleavage kinetics of sucrose with acid and enzyme catalysts. The experiment involves dissolving sucrose in water and mixing it with hydrochloric acid and monochloroacetic acid solutions in polarimeter tubes. Polarization readings are taken over time to determine the rate constants and specific rotation.

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    Report (Chemical Kinematics) : Name: Muhammed Aadil Khan (M.A.K) Reg No: 21416042 Group: A Date: Aim

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    This document is a report on an experiment to calculate the specific rotation of sucrose using a polarimeter and to observe the cleavage kinetics of sucrose with acid and enzyme catalysts. The experiment involves dissolving sucrose in water and mixing it with hydrochloric acid and monochloroacetic acid solutions in polarimeter tubes. Polarization readings are taken over time to determine the rate constants and specific rotation.

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    This document is a report on an experiment to calculate the specific rotation of sucrose using a polarimeter and to observe the cleavage kinetics of sucrose with acid and enzyme catalysts. The experiment involves dissolving sucrose in water and mixing it with hydrochloric acid and monochloroacetic acid solutions in polarimeter tubes. Polarization readings are taken over time to determine the rate constants and specific rotation.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    30 views2 pages

    Report (Chemical Kinematics) : Name: Muhammed Aadil Khan (M.A.K) Reg No: 21416042 Group: A Date: Aim

    Uploaded by

    ash
    This document is a report on an experiment to calculate the specific rotation of sucrose using a polarimeter and to observe the cleavage kinetics of sucrose with acid and enzyme catalysts. The experiment involves dissolving sucrose in water and mixing it with hydrochloric acid and monochloroacetic acid solutions in polarimeter tubes. Polarization readings are taken over time to determine the rate constants and specific rotation.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    of 2

    Report (chemical kinematics)

    Name : Muhammed Aadil khan (M.A.K)

    Reg No : 21416042

    Group : A

    Date :

     Aim : Calculate the specific rotation of sucrose using a


    Polarimeter.
     Observe the cleavage kinetics of sucrose with an acid
    catalyst, hydrochloric acid.
     Observe the cleavage kinetics of sucrose with an enzyme
    catalyst, invertase.
     Calculate the rate constant for each run from the
    rotational readings

    Experimental :

    Apparatus. Polarimeter (described in the Appendix); sodium-


    vapor lamp; thermostat and
    circulating pump; two water-jacketed polarimeter tubes; two
    #3 rubber stoppers; pure sucrose;
    100 ml of 4 M hydrochloric acid; 100 ml of 4 M
    monochloroacetic acid; three 25-ml pipettes.

    Procedure. Twenty grams of pure cane sugar (sucrose) is


    dissolved in water (filtered, if
    necessary, to give a clear solution) and diluted to 100 ml. The
    sucrose solution, the 4 M
    hydrochloric acid solution, and the 4 M monochloroacetic
    acid are placed in the 25° thermostat
    and allowed to stand a few minutes to come to temperature.
    Two jacketed polarimeter tubes are connected in series with
    the circulating water from a
    thermostat at 25° . A zero reading is taken.
    Twenty-five milliliters of the sucrose solution is thoroughly
    mixed with 25 ml of the 4 M
    monochloroacetic acid solution, and small portions of the
    solution are used to rinse out one
    polarimeter tube. The tube is then filled and stoppered. The
    second polarimeter tube is filled in
    a similar manner after rinsing, using 25 ml of the sugar
    solution and 25 ml of the 4 M
    hydrochloric acid solution. The tubes are filled rapidly, and
    readings are taken as soon as
    possible after mixing. The first reading is taken on the
    hydrochloric acid solution and recorded,
    and subsequent measurements are recorded about every 10
    min for the first hour or so. As the
    reaction slows down, the observations may be taken less
    frequently. The observations should
    extend over a period of 3 hr or more. The reaction goes much
    more slowly with the
    monochloroacetic acid, and the readings are taken less
    frequently. Monochloroacetic acid
    readings are taken at convenient intervals of time when the
    polarimeter is not needed for the
    hydrochloric acid solution.

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