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    Dexamethasone Tds PDF

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    Clark Llamera
    Dexamethasone is a synthetic glucocorticoid that inhibits NF-kB and MAPK activation through TLR engagement. It is provided as a white powder that is soluble in ethanol and can be prepared as a stock solution of 1 mg/ml. Dexamethasone pretreatment of cells at 100 nM inhibits NF-kB and MAPKs and can be used to assess the role of these pathways in TLR signaling.

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    Dexamethasone Tds PDF

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    Clark Llamera
    66 views1 page
    Dexamethasone is a synthetic glucocorticoid that inhibits NF-kB and MAPK activation through TLR engagement. It is provided as a white powder that is soluble in ethanol and can be prepared as a stock solution of 1 mg/ml. Dexamethasone pretreatment of cells at 100 nM inhibits NF-kB and MAPKs and can be used to assess the role of these pathways in TLR signaling.

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    Dexamethasone is a synthetic glucocorticoid that inhibits NF-kB and MAPK activation through TLR engagement. It is provided as a white powder that is soluble in ethanol and can be prepared as a stock solution of 1 mg/ml. Dexamethasone pretreatment of cells at 100 nM inhibits NF-kB and MAPKs and can be used to assess the role of these pathways in TLR signaling.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    66 views1 page

    Dexamethasone Tds PDF

    Uploaded by

    Clark Llamera
    Dexamethasone is a synthetic glucocorticoid that inhibits NF-kB and MAPK activation through TLR engagement. It is provided as a white powder that is soluble in ethanol and can be prepared as a stock solution of 1 mg/ml. Dexamethasone pretreatment of cells at 100 nM inhibits NF-kB and MAPKs and can be used to assess the role of these pathways in TLR signaling.

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    © All Rights Reserved
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    of 1

    Dexamethasone

    NF-kB and MAPK inhibitor


    Catalog # tlrl-dex

    For research use only


    Version # 13K15-MM

    PrODucT iNFOrMATiON METHODS


    content: Preparation of stock solution (1 mg/ml)
    100 mg Dexamethasone To obtain a 1 mg/ml stock solution:
    1. Weigh 5 mg of dexamethasone.
    Storage and stability: 2. Add 5 ml ethanol to 5 mg dexamethasone. This provides a
    - Dexamethasone is shipped at room temperature. Store solution at 1 mg/ml (2.55 mM).
    at 4°C. Product is stable for 1 year when properly stored. 3. Vortex until complete solubilization.
    - Solutions of dexamethasone are stable for 1 month at 4°C. 4. Aliquote and store at 4˚C.
    Protect from light. 5. Once dexamethasone is solubilized, dilutions can be
    prepared by adding sterile culture medium. To obtain a
    solution at 20 mg/ml (51 mM), add 49 ml of culture medium
    DEScriPTiON to 1 ml of the stock solution. Aqueous solutions should not be
    Dexamethasone is a synthetic glucocorticoid compound with stored for more than one day.
    potent anti-inflammatory activities. It represses a large set of
    pro-inflammatory genes by blocking NF-kB and MAPK Working concentration: 100 nM (40 ng/ml)
    activation during TLR engagement inhibits NF-kB and
    specific MAPKs depending on the TLR ligand and whether the NF-kB and MAPK inhibition:
    adapters MyD88 and TRIF are activated individually or To assess the role of NF-kB and MAPK, pretreat cells, such as
    coincidently1. macrophages or HEK293 cells transfected with one or several
    TLR genes, for 1 hour with or without dexamethasone
    1. Bhattacharyya S. et al., 2010. TAK1 targeting by glucocorticoids at 100 nM and then incubate at 37°C with the appropriate TLR
    determines JNK and IkappaB regulation in Toll-like receptor- ligand for 1 to 6 hours. Detect NF-kB and MAPK inhibition
    stimulated macrophages. Blood. 115(10):1921-31. using the appropriate detection system.

    cHEMicAl PrOPErTiES rElATED PrODucTS


    cAS number: 50-02-2
    Synonym: Prednisolone F Product catalog code
    Formula: C22H29FO5
    Molecular weight: 392.46
    HO
    OH
    Molecular Weight: 228,24
    Chemical Formula: C14H12O3
    pUNO1-hTLR2 (plasmid with human TLR2 gene) puno1-htlr2
    Solubility:Dexamethasone
    OH

    n° CAS 501-36-0
    Resveratrol

    Molecular Weight: 331,34

    Piceatannol
    Chemical Formula: C20H14FN3O
    F

    N
    OH
    N
    H
    N
    n° CAS 152121-30-7

    SB202190

    pUNO-mTLR2 (plasmid with mouse TLR2 gene) puno-mtlr2


    Molecular Weight: 475,62
    Chemical Formula: C26H41N3O5

    O O
    H H
    O N N
    N H
    H
    O O

    n° CAS 133407-82-6
    MG 132

    Molecular Weight: 244,24


    Chemical Formula: C14H12O4
    O OH
    F H HO
    H3 C H CH3 OH
    HO OH
    CH3
    O

    OH OH
    n° CAS 10083-24-6
    n° CAS 50-02-2

    Dexamethasone Piceatannol

    approximately 25 mg/ml in ethanol


    Pam3CSK4 (TLR2 ligand) tlrl-pms
    Appearance: White powder
    n° CAS 50-02-2 pUNO-hTLR3 (plasmid with human TLR3 gene) puno-htlr3
    pUNO-mTLR3 (plasmid with mouse n°
    TLR3 gene)
    CAS puno-mtlr3
    10083-24-6
    OH OH
    Poly(I:C) HMW (TLR3 ligand) tlrl-pic

    O
    CH3
    HO OH
    H3 C H CH3 OH

    F H HO
    O OH

    Chemical Formula: C14H12O4


    Molecular Weight: 244,24

    TECHNICAL SUPPORT
    MG 132
    InvivoGen USA (Toll‑Free): 888‑457‑5873
    InvivoGen USA (International): +1 (858) 457‑5873
    n° CAS 133407-82-6
    InvivoGen Europe: +33 (0) 5‑62‑71‑69‑39 www.invivogen.com
    InvivoGen Hong Kong: +852 3622‑3480
    E‑mail: [email protected]
    O O
    H
    N H
    O N N

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