BD Accuri™ C6 Cytometer

Flow cytometry within reach.™

Flow Cytometry within Reach
The BD Accuri™ C6 is a personal flow cytometer that brings cell analysis
within reach by being easy to use, simple to maintain, and affordable.
The analytical power and versatility of Many BD Accuri C6 cytometer users can
today’s laser-based flow cytometry systems begin collecting and analyzing data with the
have unlocked the mysteries of cell biology help of a quick start guide. The intuitive
and empowered entirely new fields of interface of the software guides the user
research. As a result, flow cytometry has through workflows. A wide dynamic range
become a staple of modern laboratories of over 6 decades ensures that all data is
around the world. Innovations in ease of use available all of the time. Information
reflected in the BD Accuri C6 cytometer make obtained from the BD Accuri C6 can be
these powerful capabilities more accessible re-analyzed at any time if gating or
to a new generation of flow cytometry users. compensation changes are required, or
to accommodate new research.
The compact footprint and portable weight
of the BD Accuri C6 also make it a valuable The BD Accuri C6 is small enough to easily
personal use tool for experienced researchers fit on a benchtop and can be placed in a
who want a cytometer to be easily available laminar flow hood if biohazard containment
when and where they need it. is required. It measures 11 x 14.75 x 16.5
inches (H x W x D) (27.9 x 37.5 x 41.9 cm) and
weighs just 30 pounds (13.6 kg).

3
 Pre-Optimized Detectors,
Minimized Setup Time
The system is equipped with a blue and red laser, two light Data is digitally collected over a 6-decade dynamic range
scatter detectors, and four fluorescence detectors with optical (16 million channels of digital data), making all data available
filters optimized for the detection of FITC, PE, PerCP, and APC. to users as needed. Gating strategies and fluorescence
A compact optical design, fixed alignment, and pre-optimized compensation values can be set before, during, or after
detector settings make the system easier to use. data collection. After data is collected, the BD Accuri™ C6
software Zoom function allows visualization of data at any
Optional filters and the Selectable Laser Module expand the
scale, so that users can precisely set gates and regions.
available fluorochrome combinations. During manufacture,
laser and optical alignments are set and locked down. The Should updates in the values be required later, or if optimi-
result is that each BD Accuri C6 cytometer is manufactured zation is needed, simply change the settings and re-analyze
with standardized fluorescence performance so that users the data. This flexibility also allows data to be re-examined
do not need to adjust detector voltages. to accommodate new research findings.

The system has been put through intense testing to ensure

that the design can withstand rugged conditions. Provided
the system is anchored, it can run samples even if the
benchtop is in motion, for example, onboard a ship.

A06 CD4_CD8_CD3 A06 CD4_CD8_CD3

Gate: (P1 in all) Gate: (P1 in all)
10 6

10 6

CD3+ CD4+
CD3+ CD8+ 33.6%
10 5
10 5

7.1%
10 4
10 4
PE-Cy7 CD8-A

PE CD4-A
10 3
10 3

10 2
10 1.7

10 1

10 1.7 10 3 10 4 10 5 10 6 10 1.7 10 3 10 4 10 5 10 6
APC CD3-A APC CD3-A
A07 CD45_CD4_CD8_CD3 A07 CD45_CD4_CD8_CD3
T-cell phenotyping, 4-color analysis Gate: (CD3+ CD8+ in (P1 in all)) Gate: (CD3+ CD4+ in (P1 in all))
100

300

Thawed human PBMCs were stained with directly labeled anti- V1-L V1-R V2-L V2-R
15.5% 84.5%
CD45RA FITC, CD4 PE, CD8 PE-Cy™7, and CD3 APC in PBS + 1 mg/
mL of BSA, covered, on ice, for 30 minutes. Cells were acquired and
200

gated on lymphocytes to identify CD3+CD8+ cytotoxic (blue) and

50

CD3+CD4+ helper (red) T-cell populations.

Count

Count
100

After FITC staining, subpopulations of CD45RA+ and CD45RA–

cytotoxic and helper T cells were identified.
0

10 2 10 3 10 4 10 5 10 6 10 2 10 3 10 4 10 5 10 6
FITC CD45RA-A FITC CD45RA-A

4
 OPTICS

A B C
A02 K562 untreated JC-1 JC-1JC-1 A02 K562
A02A02untreated
K562K562 JC-1 JC-1JC-1
untreated
untreated A03 K562
A03A03CCCP
K562K562JC-1
CCCPCCCP
JC-1JC-1
Apoptosis detection A02A02
K562K562
untreated
untreated
5,093,085

Gate:Gate:
(P1Gate:
in(P1
all) Gate:Gate:
(P1Gate:
in(P1
all)
5,093,085
5,093,085

Gate:Gate:
[No Gating]
Gate:
[No[No
Gating]
Gating] (P1
in all)
in all) (P1
in all)
in all)
10 7

10 7
10 7

10 7

10 7

10 7

The BD Accuri C6 can perform most flow

cytometric apoptosis assays, including
Annexin V, caspase activation, PARP cleavage,
P9 P9 P9 P9 P9 P9
mitochondrial membrane potential (ΔΨm), P1 P1 P1 87.6%
87.6%
87.6% 12.9%
12.9%
12.9%
90.0%
90.0%
90.0%
JC-1 FL-2-A

JC-1 FL-2-A
10 6
JC-1 FL-2-A
JC-1 FL-2-A

10 6
JC-1 FL-2-A
JC-1 FL-2-A
10 6

10 6

10 6

10 6

and DNA fragmentation. (A) K562 cells

2,000,000

2,000,000
2,000,000
SSC-A

SSC-A
SSC-A

(human chronic myelogenous leukemia)

were treated with CCCP to induce apoptosis,
P10 P10P10 P10 P10P10
then stained with JC-1 according to the 10.6% 10.6%
10.6% 84.0%
84.0%
84.0%
10 5.1

10 5.1
10 5.1

10 5.1

10 5.1

10 5.1
0

0
0

BD™ MitoScreen (JC-1) protocol (Cat. No. 0 0 05,000,000 10,000,000

5,000,000
5,000,000 14,540,969
10,000,000
10,000,000
14,540,969 10 4.6
14,540,969 10 4.6
10 5
10 4.6 10 5 10 5 10 6 10 6 10 6 10 6.8 10 6.8
10 6.8 10
4.6 10 4.6
10 5
10 4.6 10 5 10 5 10 6 10 6 10 6 10 6.8 10 6.8
10 6.8
FSC-AFSC-A
FSC-A JC-1 FL-1-A
JC-1JC-1
FL-1-A
FL-1-A JC-1 FL-1-A
JC-1JC-1
FL-1-A
FL-1-A
551302). The cells were washed and collected
on the BD Accuri C6. CCCP treatment (C)
resulted in a shift in ΔΨm (red to green) vs
untreated cells (B), indicating apoptosis.

5
 Sample Flexibility with Optional
Walkaway Sample Loading
A unique low-pressure pumping system drives the fluidics. The non-pressurized system supports any brand of 12 x
A sheath-focused core enables event rates of up to 10,000 75-mm (or smaller) sample tubes, including microcentrifuge
events per second and a sample concentration of over tubes and tubes made of polypropylene or polystyrene. The
5 x 106 cells per mL. In addition, the system derives sample BD Accuri C6 cytometer simplifies system maintenance with
volume and can calculate absolute counts or sample automatic cleaning cycles on instrument shutdown. The
concentration per microliter. system can employ laboratory-grade water for sheath fluid,
reducing operating costs.

For walkaway convenience, the optional BD CSampler™

accessory offers reliable and easy-to-use automation. The
system supports 48- and 96-well plates and deep-well plates,
and is also supplied with a 24-tube rack for standard 12 x
75-mm tubes. They are processed directly in the BD Accuri
C6 cytometer, saving time. The BD CSampler adds minimal
footprint to the BD Accuri C6, about three feet square for
the pair, keeping the benchtop free for other uses.

To streamline sample processing, the BD CSampler allows

multiple collection settings to be applied to plate or tube
runs. To process a sample immediately, a run can be paused
using the Interrupt function. When the priority operation
is complete, the original plate can be returned to the
BD CSampler to resume the original run. Easy-to-read
software messages keep users informed of system status.

A. Beckman
A Beckman Coulter
Coulter Cyan ADP
Cyan ADP
Gap-free analysis of intracellular calcium
A The three left-hand cytograms show calcium data obtained on 104 104 104

a Beckman Coulter CyAn™ ADP using the “stop-flow” method,

103 103 103
488: 530/40: Fluo-4

488: 530/40: Fluo-4

488: 530/40: Fluo-4

showing time gaps when control and test compounds were added.

B The three right-hand cytograms show data obtained on a 102 102 102
BD Accuri C6, adding the same compounds in open Eppendorf®
tubes without interrupting sample acquisition. No time gaps were 101 101 101
observed; otherwise, both methods obtained comparable data.
Data from Vines A, McBean GJ, Blanco-Fernández A. A flow cytometric method for continuous 100 100 100
measurement of intracellular Ca2+ concentration. Cytometry Part A. 2010;77:1091-1097; 0 sec 2 min 30 sec 5 min 7 min 30 sec 10 min 0 sec 2 min 30 sec 5 min 7 min 30 sec 10 min 0s
Time Time
reproduced courtesy of the authors.
A23187 A23187 T

B. BD Accuri C6
Gate: [No Gating] Gate: [No Gating]
7.2

7.2

7.2
10

10

10

6
6

6
10

10

10
-4

-4

-4
5

5
 FLUIDICS

Applications in kinetic analysis of cellular responses

Changes to intracellular calcium (Ca2+) levels can occur
rapidly, in some cases within nanoseconds of stimulation,
and obtaining accurate data is a significant research
challenge. To add test compounds to the cell suspension,
a “stop-flow” method is often used in which sampling is
paused, the sample tube opened, the agonist added, and
the tube resealed. This technique leaves a gap in data
collection that may miss essential changes in Ca2+ levels.

The BD Accuri C6 employs non-pressurized peristaltic pumps

in an open fluidics system. Open tubes allow convenient
addition of test compounds to the cell suspension without
interrupting sampling. This “continuous-flow” method
A.A.Beckman
A.
Beckman
Beckman
Coulter
Coulter
Coulter
Cyan
Cyan
Cyan
ADPADP ADP
enables non-stop analysis of calcium flux and other kinetic 4 4 4 4 4 4
10 10 10 10 10 10 104 104 104
cellular responses, such as pH, reactive oxygen and nitro-
gen species, mitochondrial membrane potential, and
10 10 10 10 10 10 3 3 3 3 3 3 103 103 103
488: 530/40: Fluo-4
488: 530/40: Fluo-4
488: 530/40: Fluo-4

488: 530/40: Fluo-4

488: 530/40: Fluo-4
488: 530/40: Fluo-4

488: 530/40: Fluo-4

488: 530/40: Fluo-4
488: 530/40: Fluo-4

nanoparticle uptake. 10 10 10 10 10 10 2 2 2 2 2 2 102 102 102

101 101 101 101 101 101 101 101 101

100 100 100 100 100 100 100 100 100

0 sec0 sec
20min
sec
2 min
30 2sec
30
minsec
530
min
sec
5 min
75min
min
7 min
30 7sec
30
minsec
10
30min
sec
10 min
10 min
0 sec0 sec
20min
sec
2 min
30 2sec
30
minsec
530
min
sec
5 min
75min
min
7 min
30 7sec
30
minsec
10
30min
sec
10 min
10 min
0 sec0 sec
20min
sec
2 min
30 2sec
30
minsec
530
min
sec
5 min
75min
min
7 min
30 7sec
30
minsec
10
30min
sec
10 min
10 min
TimeTimeTime TimeTimeTime TimeTimeTime

A23187
A23187
A23187 A23187
A23187
A23187 Thapsigargin
Thapsigargin
Thapsigargin
A23187
A23187
A23187

B.BB.
BD
BDB.
BDAccuri
BDAccuri
Accuri
Accuri C6C6C6
C6
Gate:
Gate:
[No
Gate:
[No
Gating]
[No
Gating]
Gating] Gate:
Gate:
[No
Gate:
[No
Gating]
[No
Gating]
Gating] Gate:
Gate:
[No
Gate:
[No
Gating]
[No
Gating]
Gating]
7.2
7.2
7.2

7.2
7.2
7.2

7.2
7.2
7.2

104
10

10

10

10

10

10

10

10

10
6
6
6

6
6
6

6
6
6
10

10

10

10

10

10

10

10

10

103
488: 530/40: Fluo-4

488: 530/40: Fluo-4

104Fluo-4

Fluo-4

488: 530/40: Fluo-4

104Fluo-4

Fluo-4

488: 530/40: Fluo-4

104Fluo-4

Fluo-4
105

5
5

105

5
5

105

5
5
10

10

10

10

10

10

102
4
4

4
4

4
4
10

10

10

10

10

10
102 530/40:

488:2 530/40:

102 530/40:

488:2 530/40:

102 530/40:

488:2 530/40:
103

3
3

103

3
3

103

3
3
10

10

10

10

10

10

101
2

2
1488:

1488:

1488:
10

10

10

10

10

10

100
1
1

1
1

1
1
10

10

10

10

10

10

10

10

10

ec 10 min 0 sec 2 min 30 sec 5 min 7 min 30 sec 10 min 0.0s0.0s 0.0s 5m 5m
0.0s0.0s
5m 0.0s 10m10m
0.0s0.0s 0.0s0.0s0.0s 0.0s
10m 5m 5m
0.0s0.0s
5m 0.0s 10m10m
0.0s0.0s 0.0s0.0s0.0s 0.0s
10m 5m 5m
0.0s0.0s
5m 0.0s 10m10m
0.0s
10m
0.0s 0.0s
Time Time
TimeTime Time
TimeTime Time
TimeTime
Thapsigargin A23187 A23187 EtOH
A23187
A23187 EtOH
EtOH A23187
A23187
A23187 Thapsigargin
Thapsigargin
ThapsigarginA23187
A23187
A23187

Gate: [No Gating]

7.2
10

7
610
-4
5
 Intuitive Software—Master in Minutes
BD Accuri C6 software has an intuitive user interface that Sample data can be customized in the Statistics tab. Data
was developed based on hundreds of hours observing is displayed in a master table, and statistics can be easily
researchers using flow cytometers. copied and pasted into spreadsheets to facilitate reporting.
To simplify creating presentations, plots can be imported
As a result, most novice flow cytometry users find it so easy
into Microsoft® Office applications using drag and drop.
to use, that they can collect and analyze data in less than an
BD Accuri C6 software supports live gating, event coloring,
hour. Software options and instrument controls are clearly
export of publication-quality, vector-scalable graphics,
visible from the software’s tabbed interface which enables
and batch analysis, for review or modification of multiple
access to the collection, analysis, and statistics functions.
samples for the automatic creation of Microsoft
Data is acquired from the Collect tab. Users can create new PowerPoint® and Excel files.
plots, or copy and re-use plots from this tab. The software
BD Accuri C6 software files can be exported in FCS 3.0 format
supports a full range of selection regions including rectan-
for seamless importing of user data into flow cytometry
gular, polygon, quadrant, horizontal, and vertical markers.
analysis programs including FCS Express and FlowJo™.
The Analyze tab displays plots and samples in any combination.
In the Analyze tab, users can create color histogram overlays,
print multiple plots, and compare samples. Use the Zoom tool
to magnify areas of data, instead of voltage adjustments to set
the channel range viewed, to better visualize results.

Gate: (P1 in all)

1,500
Control

1,000
SSEA-4
Collect tab
Count

SSEA-1
500
0

10
1 10
2 10
3 10
4 10
5 10
6 10
7.2

Sample figure creation using Gate: (P1 in all)

Gate: (P1 in all) Gate: (P1 in all) Gate: (P1 in all)
1,500

Gate: (P1 in all)

1,500

1,500

1,500
1,500

BD Accuri C6 software
Control Control
Embryonal carcinoma cell line 2102Ep Control Control
was stained with antibodies corres-
1,000

Control
1,000

1,000

1,000
1,000

ponding to the proteins indicated on TRA-1-60 TRA-1-60

SSEA-4 SSEA-4
the histograms. Data was collected on TRA-1-60
Count

Count

a BD Accuri C6 cytometer. Data shows

Count

Count
Count

SSEA-1 SSEA-1 SSEA-4

500

500

500

500
500

overlays of single-parameter plots

after fluorescence compensation has
been applied. Labeling on plots was
added in presentation software after
0

0
0

1 102 103 4
1 5
2 63 47.2 5 6 7.2 1 2 3 4
1 5
2 63 47.2 5 1
6 27.2 3 4 5 6 7.2
plots were dragged and dropped 10
in 10
10 10
10 10
10 10
10 10 10 10 10 10 10 10
10 10
10 10
10 10
10 10 10
10 10
10 10 10 10 10 10

from the BD Accuri C6 software file.

8 Gate: (P1 in all)

Gate: (P1 in all) Gate: (P1 in all)
Gate: (P1 in all)
1,500

2,000
1,500

2,000
 SOFTWARE

Statistics tab Analyze tab Batch Analysis tab

A B C
Stem cell marker comparisons facilitated
1,000
1,000
1,000

1,000
1,000
1,000

800
800
800

with overlay plots

The human embryonal carcinoma (EC) cell line
Neg Neg
Neg Neg Neg
Neg
2102Ep can serve as a reference standard for
600
600
600

CD147
CD147
CD147 control SSEA-4
control
control SSEA-4
SSEA-4
Neg Neg
Neg control
control
control
human embryonic and induced pluripotent control
control
control
Partial data displayed
Partial data displayed
Partial data displayed

Partial data displayed

Partial data displayed
Partial data displayed

Partial data displayed

Partial data displayed
Partial data displayed

stem cells because it expresses most of the same Oct 3/4

Oct 3/43/4
Oct
Count500
Count500
500

Count500
Count500
500

200 Count400
200 Count400
400

markers. After staining with antibodies to stem

Count

Count

Count

TRA-1-60
TRA-1-60
TRA-1-60
cell markers, 2102Ep EC cells were collected
200

and analyzed on a BD Accuri C6. Overlays of

single-parameter histograms were drawn with SSEA-1
SSEA-1
SSEA-1
BD Accuri C6 software. Plots were dragged and
0
0
0

0
0
0

0
0
0

10 3 10 310 3 10 410 410 4 10 5 10 510 5 10 6 10 610 6 10 7.2 10 7.2

10 7.2 10 2 10 210 2 10 3 10 310 3 10 4 10 410 4 10 5 10 510 5 10 6 10 6.5
106 1066.5
10 10 6.5 10 1.7 10 1.7
10 1.7 10 3 10 310 3 10 4 10 410 4 10 5 10 510
1055.7 10 5.7
10 5.7
dropped into presentation software, where labels FL1FL1
(Multiple
FL1 names)
(Multiple
(Multiple names)
names) FL2FL2
(Multiple
FL2 names)
(Multiple
(Multiple names)
names) FL4FL4
(Multiple
FL4 names)
(Multiple
(Multiple names)
names)
were added. Note: The curve for SSEA-1 (gray
in plot A), a negative pluripotency marker, is
almost identical to the negative control (black).

9
 APPLICATIONS

Easy to Learn and Use—

Fits a Wide Range of Applications
Multiplexed soluble protein quantitation
With BD™ Cytometric Bead Array (CBA) reagents, the
BD Accuri C6 flow cytometer, and FCAP Array™ analysis
software, users can quantify multiple proteins simultaneously,
using the broad dynamic range of fluorescence detection
offered by flow cytometry and antibody-coated beads. This
method significantly reduces sample volume requirements
and time to results in comparison with traditional ELISA and
Western blot techniques. BD CBA Kits take advantage of the
standard BD Accuri C6 configuration. The optional Selectable
Laser Module available for the BD Accuri C6 introduces
flexibility, enabling detection of two parameters from the
red laser, a requirement for BD CBA Flex Sets.

A
A01 A08
Gate: R1
6.2
10
FL3 780-60BP-A
5 10
3.7 104
10

Quantitation of mouse IL-17A on the BD Accuri C6 10 3.7 5

10
FL4 675-25BP-A
10 6 6.6
10

The BD CBA portfolio includes assays for measurement of soluble

and intracellular proteins, including cytokines, chemokines, and
growth factors involved in the immune response. BD CBA solutions B C5 - ES Mouse IL-17A
R1=100.00% A: 6.923 B: 1.795 C: 14.234 D: 31.766 E: 5.147
are available in two formats. BD CBA Kits are preconfigured for Fitting type: 5 Parameter Logistic
MFI

ultimate ease of use with routine panels, while BD CBA Flex Sets
provide an open and configurable method of detection, so that 100000
researchers can build their own multiplexes.

A BD CBA Mouse Enhanced Sensitivity (ES) 11-plex resolved on 10000

the BD Accuri C6.

B Standard curve (concentration vs median fluorescence intensity) 1000

of the BD CBA Mouse ES IL-17A Flex Set 6 using FCAP Array 3.0
analysis software.
0
100 1000 10000 100000
Concentration

10
 Efficient gene expression analysis The BD Accuri C6 simplifies the detection of fluorescently
Screening thousands of cells for reporter gene expression tagged genes incorporated into cells. It can also measure the
levels is fundamental to understanding how genes are effects of gene knockdown when RNA interference (RNAi) is
regulated inside the cell. Advances in flow cytometry and used to reduce gene expression. With multiparameter flow
a full spectrum of fluorescent proteins now available allow cytometry, one fluorescent antibody can be used to measure
biomedical researchers to more quickly, easily, and affordably the intracellular level of the transfected siRNA target
leverage this technology in gene expression analysis. protein, while others can measure the cells’ functional
responses such as up- and downregulation of surface
Fluorescent proteins have come a long way since the
markers or intracellular protein phosphorylation.
original application of Green Fluorescent Protein (GFP) for
the detection of gene expression. Fluorescent proteins now
span the entire spectrum from short violet to long red, and
can be used to study a wide variety of cellular phenomena. E. coli wild type
3 103.4

3 103.4

3 103.4

3 103.4
400

400
10

10

10

10
P2 P2 P2 P2 P2
300

300

52.0% 52.0% 53.0% 53.0% 53.

Partial data displayed

Partial data displayed

20000 / 100000 (20%)

20000 / 100000 (20%)

events displayed

events displayed
2

2
200

200
10

10

10

10
SSC-H

SSC-H

Count

Count

SSC-H

SSC-H
M1 M1
0.1% 0.1%
100

100
1

1
10

10

10

10
0.3

0.3

0.3

0.3
0

0 1 0 102101 103102 104103 105104106105 6

Detection of GFP expression in bacteria 2 2 3 3 4.1 4.1
10

10

10

10

10 10 10 10
10 10 10 10 10 10 2 2
FL1: 530
FL1:BP
530 BP
10 10
Two E. coli cultures, one wild type and the other transfected with a
constitutive GFP-expressing plasmid, mixed in a 1:1 ratio.
Data courtesy of Tim F. Cooper, Department of Biology and Biological Chemistry, University
E. coli + GFP plasmid
of Houston, Houston, TX.
3,200
3 103.4

103.4
400
400

10 10

3 10 10
10

10

P2
P2 P2
P2 P2
P2 P2
P2
300
300

52.0%
52.0% 53.0%
53.0% 53.0%
53.0% 54.9%
54.9%
displayed
data displayed
(20%)
100000 (20%)

41956 / 209780 (20%)

Partial data displayed

2,000
displayed
events displayed

events displayed
2

2
200

10

10
200

SSC-H
10

10
20000 // 100000

Count
Count

SSC-H

Count
Partial data
events

M1
M1
events

1,000

M1
0.1%
Partial

0.1%
20000

100

41956
100

93.6%
Partial
1

1
10

10
10

10
0.3

0.3
0.3

0.3
00

00

22 33 4.1 00 11 22 33 44 55 66 22 3
10

10

4.1
10

10

1010 1010 1010 1010 10

10 10
10 10
10 10
10 10
10
22 3 4.1 10
10
0 10
10
1 2
10
10
3
10
10
44
10
10
55
10
10
66
10
10
10
10 10
10
FL1: 530
FL1: 530 BP
BP
10
10 10
10 10
10
FSC
FSC
FSC-H FL1: 530 BP
11
 Special Order Products for Unique Needs
The BD special order program allows customers to purchase To support the evolving needs of researchers, the BD Accuri
instruments configured to fit precise research and assay C6 can be custom configured by the BD special order team
needs. This innovative program is tailored to the special needs with a wide choice of innovative lasers. This expanded level of
of research at the leading edge of biomedical discovery, and flexibility and choice helps make the special order BD Accuri
offers a wide range of choices to help researchers create the C6 fit a wider choice of research application requirements.
ultimate customized instrument for their requirements.
Your BD Sales Representative is your point of contact to discuss
a special order product for your lab. Together with an engineer
from the special order team, they will follow a structured
process to define, design, and manufacture an instrument
that exactly matches your requirements. Strict manufacturing
controls are in place to ensure high-quality outcomes.

Analysis of DNA content and

ploidy in plants 4C
Arabidopsis thaliana root tissues A B C
106 Gated on P1
were chopped with a fresh razor 750
Number of Nuclei

blade, stained with PI, and acquired R1

105 106
on the BD Accuri C6. On an FL2 vs
FL3-A

FL3-A

500 2C
6C
FL3 plot, nuclear events cluster in 104
a narrow diagonal region (A, B). P1
1.8% 250 16C
Gating on these nuclear events, FL2 103
fluorescence (C) shows clear peaks 105
32C
corresponding to cell ploidy. 103 104 105 106 104 105
102 103 104 105 106
Data courtesy of David W. Galbraith, School of FL2-A FL2-A FL2-A
Plant Sciences and Bio5 Institute for Collabora-
tive Bioresearch, University of Arizona, Tucson,
AZ, USA.

12
CUSTOM CONFIGURATION

Speed analysis of marine and freshwater samples The BD Accuri C6 can help speed sample analysis for biologists
Environmental research in marine and freshwater studying marine and freshwater ecosystems. The system
ecosystems is predominantly focused on the microbiomes is portable enough to travel aboard ship and can handle
of those aquatic environments. Of critical concern to particles as small as 0.5 μm, quickly delivering multiparametric
environmental research is the primary productivity of measurements of particle size and autofluorescence in both
phytoplankton and the distribution of phytoplankton and cultures and environmental samples.
cyanobacteria species responsible for harmful algal blooms.

Site
Site 11 Site
Site 22
7.2

7.2
7.2

7.2
fluorescence

106 1010

106 1010
a,bfluorescence
670LPLP

P7 P7
5 5 10

5 5 10

P7 P7
em:670

50.0%
50.0% 70.4%
70.4%
104 1010

104 1010
488;em:

4
Chlorophylla,b

3 3 10

3 3 10
ex:488;

P6
P6 P6
P6
Chlorophyll

42.8% 22.8%
102 1010

102 1010

42.8% 22.8%
FL3ex:

2
FL3

1 1 10

1 1 10
1010

1010

1011 1022 1033 1044 1055 1066 107.2 1011 1022 1033 1044 1055 1066 107.2
10 10 10 10 10 10 107.2 10 10 10 10 10 10 107.2
Phycocyanin
Phycocyanin fluorescence
fluorescence
FL4
FL4 ex:
ex: 640;
640; em:
em: 675
675 ±12.5
±12.5 nm
nm

Analysis of aquatic microorganisms with the Site

Site 44 Site
Site 23
23
7.2

7.2

BD Accuri C6
7.2

7.2
fluorescence

106 1010

106 1010
a,bfluorescence

In water samples from four sites in Saginaw Bay,

670LPLP

Michigan, plots of phycocyanin fluorescence vs P7 P7

5 5 10

5 5 10

P7 P7
em:670

chlorophyll fluorescence were used to separate 79.1% 73.1%

73.1%
79.1%
104 1010

104 1010
488;em:

fluorescent phytoplankton into cyanobacteria (region

4

4
Chlorophylla,b

P6) and other fluorescent phytoplankton (P7).

3 3 10

3 3 10
ex:488;

Microorganism counts obtained by direct volume P6 P6

P6
P6
Chlorophyll

12.2% 17.6%
102 1010

102 1010

12.2% 17.6%
FL3ex:

on the BD Accuri C6 showed that sites 1 and 2, close

2

2
FL3

to the nutrient-rich mouth of the Saginaw River,

1 1 10

1 1 10

had at least six times more cyanobacteria than the

1010

1010

other two locations. 1011 1022 1033 1044 1055 1066 107.2 1011 1022 1033 1044 1055 1066 107.2
10 10 10 10 10 10 107.2 10 10 10 10 10 10 107.2
Data courtesy of Juli Dyble Bressie, National Oceanic and Atmospheric Phycocyanin
Phycocyanin fluorescence
fluorescence
Administration, Seattle, WA, USA. FL4
FL4 ex:
ex: 640;
640; em:
em: 675
675 ±12.5
±12.5 nm
nm

13
 SERVICES

Services and Support

BD Biosciences is fully committed to the success and Fast, easy installation
satisfaction of its customers and offers a range of options The BD Accuri C6 cytometer can be customer installed within
for BD Accuri support. just an hour of taking it out of the box. A step-by-step quick
start guide and online video simplify installation.

Preventative maintenance
Preventative maintenance procedures should be performed
every two months to change the sheath filter, pump tubing,
and fluidic filters, and to clean the SIP.

Technical application support

Our technical application support specialists are available to
provide field- or phone-based assistance and advice. Expert
in a diverse array of topics, technical application specialists
are well equipped to address customer needs in both
instrument and application support.

Training
If desired, optional hands-on training is available on
the BD Accuri C6 cytometer. The training combines flow
cytometry theory and practical skills to operate the
BD Accuri C6 cytometer.

14
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