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    Ethylenediaminetetraacetic Acid Disodium Salt Dihydrate

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    Muhamad Zunanda Aji
    This document summarizes information about ethylenediaminetetraacetic acid disodium salt dihydrate (EDTA), including its molecular formula, weight, structure, solubility properties, uses as a chelator and anticoagulant, storage instructions, and references. EDTA is a chelating agent that is soluble in water and inhibits metalloproteases. It is commonly used in electrophoresis and as an anticoagulant in blood at 1.5 mg per ml. A 0.5 M solution of EDTA at pH 8.5 is stable for 2 months at 4°C.

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    Ethylenediaminetetraacetic Acid Disodium Salt Dihydrate

    Uploaded by

    Muhamad Zunanda Aji
    35 views1 page
    This document summarizes information about ethylenediaminetetraacetic acid disodium salt dihydrate (EDTA), including its molecular formula, weight, structure, solubility properties, uses as a chelator and anticoagulant, storage instructions, and references. EDTA is a chelating agent that is soluble in water and inhibits metalloproteases. It is commonly used in electrophoresis and as an anticoagulant in blood at 1.5 mg per ml. A 0.5 M solution of EDTA at pH 8.5 is stable for 2 months at 4°C.

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    This document summarizes information about ethylenediaminetetraacetic acid disodium salt dihydrate (EDTA), including its molecular formula, weight, structure, solubility properties, uses as a chelator and anticoagulant, storage instructions, and references. EDTA is a chelating agent that is soluble in water and inhibits metalloproteases. It is commonly used in electrophoresis and as an anticoagulant in blood at 1.5 mg per ml. A 0.5 M solution of EDTA at pH 8.5 is stable for 2 months at 4°C.

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    © All Rights Reserved
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    Download as pdf or txt
    35 views1 page

    Ethylenediaminetetraacetic Acid Disodium Salt Dihydrate

    Uploaded by

    Muhamad Zunanda Aji
    This document summarizes information about ethylenediaminetetraacetic acid disodium salt dihydrate (EDTA), including its molecular formula, weight, structure, solubility properties, uses as a chelator and anticoagulant, storage instructions, and references. EDTA is a chelating agent that is soluble in water and inhibits metalloproteases. It is commonly used in electrophoresis and as an anticoagulant in blood at 1.5 mg per ml. A 0.5 M solution of EDTA at pH 8.5 is stable for 2 months at 4°C.

    Copyright:

    © All Rights Reserved
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    of 1

    Ethylenediaminetetraacetic acid disodium salt dihydrate

    Product Number E 5513


    Store at Room Temperature

    Product Description Preparation Instructions


    Molecular Formula: C10H14N2Na2O8 • 2H2O This product is slowly soluble in water at room
    Molecular Weight: 372.2 temperature up to 0.26 M, which is approximately
    CAS Number: 6381-92-6 96 mg in a final volume of 1 ml. The pH of this
    1
    pKa: 2.0, 2.7, 6.2, 10.3 solution will be approximately 4 to 6. EDTA salts are
    more soluble in water as the pH increases: the more
    Synonyms: EDTA, (Ethylenedinitrilo)tetraacetic acid EDTA there is in the salt form, the higher the pH of a
    water solution, and therefore, the higher the room
    This product is designated as Electrophoresis grade temperature solubility. This can be achieved by a
    and has been tested for use in electrophoresis buffer gradual addition of a concentrated sodium hydroxide
    systems. solution to the EDTA solution.

    EDTA is an inhibitor of metalloproteases and Storage/Stability


    metal-activated proteases, at effective concentrations A stock solution of 0.5 M EDTA at pH 8.5 is stable for
    2
    of 1-10 µM. The product acts as a chelator of the zinc months at 4 ºC.
    ion in the active site of metalloproteases, but EDTA
    can also inhibit other metal ion-dependent proteases Solutions of EDTA may be autoclaved.
    such as calcium-dependent cysteine proteases. EDTA
    may interfere with biological processes which are References
    2
    metal-dependent. 1. Data for Biochemical Research, 3rd ed., Dawson,
    R. M. C., et al., Oxford University Press (New
    For use as an anticoagulant, disodium or tripotassium York, NY: 1986), p. 404.
    salts of EDTA are most commonly used. The optimal 2. Proteolytic Enzymes: A Practical Approach, 2nd
    concentration is 1.5 mg per ml of blood. EDTA ed., Beynon, R. and Bond, J. S., eds., Oxford
    prevents platelet aggregation and is therefore the University Press (Oxford, UK: 2001), p. 322.
    3
    preferred anticoagulant for platelet counts. From a 3. Clinical Hematology: Principles, Procedures,
    2% EDTA solution, 1-2 drops per ml of whole blood Correlations. ed. Lotspeich-Steininger, C. A., et
    can be used as an anticoagulant. al., Lippincott (Philadelphia, PA: 1992), p. 18.
    4. Sorensen, K., An Easy Microtiter Plate-based
    4
    A chromogenic assay for EDTA has been reported. Chromogenic Assay for
    Ethylenediaminetetraacetic Acid and Similar
    Precautions and Disclaimer Chelating Agents in Biochemical Samples. Anal.
    For Laboratory Use Only. Not for drug, household or Biochem., 206(1), 210-211 (1992).
    other uses.
    GCY/RXR 11/02

    Sigma brand products are sold through Sigma-Aldrich, Inc.


    Sigma-Aldrich, Inc. warrants that its products conform to the information contained in this and other Sigma-Aldrich publications. Purchaser
    must determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply. Please see reverse side of
    the invoice or packing slip.

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