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PERHITUNGAN

The document discusses the calculation of standard curves and measurement of control and test samples. It presents absorbance data and concentration values at various time points to calculate absorption rate, permeability, and lag time for control and test samples.

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mufidah mawaddah
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52 views10 pages

PERHITUNGAN

The document discusses the calculation of standard curves and measurement of control and test samples. It presents absorbance data and concentration values at various time points to calculate absorption rate, permeability, and lag time for control and test samples.

Uploaded by

mufidah mawaddah
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as DOCX, PDF, TXT or read online on Scribd
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PERHITUNGAN

5.1. Kurva Baku

Konsentrasi Absorbansi
(ppm) A1 A2
5 0,0340 0,0356
10 0,1020 0,0990
15 0,1451 0,1426
20 0,2072 0,2066
25 0,3079 0,3085

Kurva Baku pH 1,2


0.35
0.3
y = 0.0129x - 0.0359
0.25
R² = 0.9782
Absorbansi

0.2
0.15

0.1

0.05

0
0 5 10 15 20 25 30
Konsentrasi (ppm)
5.2. Pengukuran Sampel Kontrol dan Uji

Absorbansi Rata-rata
Menit ke-
A1 A2
5 0,2570 0,2583 0,25765
10 0,1549 0,1567 0,1558
15 0,4322 0,4311 0,43165
30 0,2861 0,2855 0,2858
45 0,2708 0,2721 0,27145

5.1. Kurva Hubungan Qkum dengan waktu

Kontrol y = 25.687x - 7.538


R² = 0.9855
140
120
100
80
Qkum

60
40
20
0
5 10 15 30 45
t

Sampel Uji y = 23.072x - 9.296


R² = 0.9971
120

100

80

60

40

20

0
5 10 15 30 45
t
5.2. Kecepatan Absorbsi, Permeabilitas, dan Lag Time

Parameter pH 1,2
Kontrol Uji
Ka 25,687 23,072
Pm 98,8 88,73
Lag time 0,29 menit 0,4

LAMPIRAN

DATA ABSORBANSI
Waktu Rata-Rata
Absorbansi 1 Absorbansi 2 Absorbansi 3
(menit) Absorbansi
5 -0,2767 -0,2758 -0,2708 -0,2744
15 -0,2603 -0,2558 -0,2592 -0,2584
30 -0,3391 -0,3395 -0,3395 -0,3394
45 -0,3489 -0,3492 -0,3484 -0,3488
60 -0,2062 -0,2057 -0,2031 -0,2050

A. Perhitungan untuk Sampel Kontrol

1. Pengukuran Konsentrasi Sampel Kontrol

 Menit ke-5  Menit ke-30


y = 0,0129x – 0,0359 y = 0,0129x – 0,0359
0,25765 = 0,0129x – 0,0359 0,2858 = 0,0129x – 0,0359
0,0129x = 0,29355 = 0,3217
x = 22,755 ppm = 24,93 ppm
 Menit ke-10  Menit ke-45
y = 0,0129x – 0,0359 = 0,0129x – 0,0359
0,1558 = 0,0129x – 0,0359 0,27145 = 0,0129x – 0,0359
0,0129x = 0,1917 0,0129x = 0,30735
x = 14,86 ppm x = 23,82 ppm
 Menit ke-15
y = 0,0129x – 0,0359
0,43165 = 0,0129x – 0,0359
0,0129x = 0,46755
2. Perhitungan Jumlah Obat (Q)
Konsentrasi x 1mL
Menit ke-5 Menit ke-10 Menit ke-15 Menit ke-30 Menit ke-45
(Q5’) (Q10’) (Q15’) (Q30’) (Q45’)

22,755 14,86 36,24 24,93 23,82

3. Perhitungan Konsentrasi Kumulatif (Qkum)

 5’ = Q5’
t (menit) Qkum
= 22,755
 10’= Q5’+Q10
= 22,755+12,86 5’ 22,755
= 35,615
10’ 35,615
 15’= Q5’+Q10’+Q30’
15’ 71,855
= 22,755+12,86 + 36,24
= 71,855 30’ 96,785

 30’= Q5’+Q10’+Q30’+Q60’ 45’ 120.605


= 22,755+12,86 + 36,24+ 24,93
= 96,785
 45’= Q5’+Q10’+Q15’+Q30’+Q45’
=22,755+12,86 + 36,24+ 24,93+23,82
= 120,605
4. Parameter

Kecepatan Absorpsi (Ka)


y = 25,687x – 7,538
Ka = slope
Ka = 25,687/mL
Permeabilitas Membran (Pm)
𝐾𝑎
Pm =
𝐶𝑔
- Cg = 3,5 x 175 = 262,5 mg = 0,26 gram

25,687
Pm =
0,26

=98,8
Lag time
y=0
0 = 25,687x – 7,538
B. Perhitungan untuk Sampel Uji
1. Pengukuran Konsentrasi
 Menit ke-5  Menit ke-30
y = 0,0129x – 0,0359 y = 0,0129x – 0,0359
0,166665 = 0,0129x – 0,0359 0,241 = 0,0129x – 0,0359
0,0129x = 0,202565 0,0129x = 0,2769
x = 15,70 ppm x = 21,46 ppm
 Menit ke-10  Menit ke-45
y = 0,0129x – 0,0359 y = 0,0129x – 0,0359

0,19605 = 0,0129x – 0,0359 0,27525 = 0,0129x – 0,0359


0,0129x = 0,23195 0,0129x = 0,31115
x = 17,98 ppm x = 24,12 ppm
 Menit ke-15
y = 0,0129x – 0,0359
0,3173 = 0,0129x – 0,0359
0,0129x = 0,3532
x = 27,38 ppm

2. Perhitungan Jumlah Obat (Q)


Menit ke-5 Menit ke-10 Menit ke-15 Menit ke-30 Menit ke-45
(Q5’) (Q10’) (Q15’) (Q30’) (Q45’)

15,70 17,98 27,38 21,46 24,12

3. Perhitungan Konsentrasi Kumulatif (Qkum)

 5’= Q5’
t (menit) Qkum
= 15,70
 10’= Q5’+Q10’ 5’ 15,70
= 15,70+17,98
10’ 33,68
= 33,68
 15’= Q +Q +Q 15’ 61,06
5’ 10’ 30’

= 15,70+17,98+27,38 30’ 82,52

= 61,06 45’ 106,64


 30’= Q5’+Q10’+Q30’+Q60’
= 15,70+17,98+27,38+ 21,46
= 82,52
 45’= Q5’+Q10’+Q15’+Q30’+Q45’
=15,70+17,98+27,38+ 24,12
= 106,64
4. Parameter

Kecepatan Absorpsi (Ka)


y = 23.072x - 9.296
Ka = slope
Ka = 23,072/mL
Permeabilitas Membran (Pm)
𝐾𝑎
𝑃m =
𝐶𝑔
- Cg = 3,5 x 175 = 262,5 mg = 0,26 gram

23,072
Pm =
0,26

=88,73
Lag time
y=0
0 = 23.072x - 9.296
9,296
x=
23,072
x = 0,4 meni

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