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    Exp 5 HPLC

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    Anonymous XbbVyR
    This document provides a standard operating procedure for determining the caffeine content in soft drinks using high-performance liquid chromatography (HPLC). It involves preparing caffeine standards of known concentrations from 1000 ppm down to 5, 15, 25, 50, and 100 ppm. Soft drink samples are degassed under vacuum and filtered. The caffeine standards and soft drink samples are then analyzed using an HPLC with a UV detector set to 254nm, C18 column, and appropriate solvents and materials. The caffeine content in the soft drinks can be determined by comparing the samples to the caffeine standards.

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    Exp 5 HPLC

    Uploaded by

    Anonymous XbbVyR
    71 views2 pages
    This document provides a standard operating procedure for determining the caffeine content in soft drinks using high-performance liquid chromatography (HPLC). It involves preparing caffeine standards of known concentrations from 1000 ppm down to 5, 15, 25, 50, and 100 ppm. Soft drink samples are degassed under vacuum and filtered. The caffeine standards and soft drink samples are then analyzed using an HPLC with a UV detector set to 254nm, C18 column, and appropriate solvents and materials. The caffeine content in the soft drinks can be determined by comparing the samples to the caffeine standards.

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    Food analysis

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    exp 5 HPLC

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    This document provides a standard operating procedure for determining the caffeine content in soft drinks using high-performance liquid chromatography (HPLC). It involves preparing caffeine standards of known concentrations from 1000 ppm down to 5, 15, 25, 50, and 100 ppm. Soft drink samples are degassed under vacuum and filtered. The caffeine standards and soft drink samples are then analyzed using an HPLC with a UV detector set to 254nm, C18 column, and appropriate solvents and materials. The caffeine content in the soft drinks can be determined by comparing the samples to the caffeine standards.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    71 views2 pages

    Exp 5 HPLC

    Uploaded by

    Anonymous XbbVyR
    This document provides a standard operating procedure for determining the caffeine content in soft drinks using high-performance liquid chromatography (HPLC). It involves preparing caffeine standards of known concentrations from 1000 ppm down to 5, 15, 25, 50, and 100 ppm. Soft drink samples are degassed under vacuum and filtered. The caffeine standards and soft drink samples are then analyzed using an HPLC with a UV detector set to 254nm, C18 column, and appropriate solvents and materials. The caffeine content in the soft drinks can be determined by comparing the samples to the caffeine standards.

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    © All Rights Reserved
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    You are on page 1of 2

    Malaysian Institute of Chemical

    & Bioengineering Technology

    Food Technology Department Document SOP /TP/011 Version 01


    Title Determination of Caffeine in Soft Drink by HPLC Date 17/03/08
    Page 1 of 3
    1. Introduction
    Caffeine has a mildly addictive effect on the body. It is therefore interesting to know exactly how much
    caffeine is in certain beverages. One way to analyze caffeine content in beverages is by using high
    performance liquid chromatography (HPLC).

    2. Scope
    Prepare standard solution of known caffeine concentration and compared them to caffeinated sample (soft
    drink)

    3. Procedure
    3.1 Equipment and Supplies:
    Chemicals
    • HPLC solvent /mobile phase
    • Caffeine standard
    • Sample (soft drink)

    Materials
    • HPLC UV detector (254nm)
    • Column C18 Nova Pack
    • Vacuum filter
    • Syringe & micro syringe
    • Micro filter paper
    • Volumetric flasks/ small vial for filter samples

    3.2 Preparation of caffeine standard (stock solution)


    a. Calculate for prepare caffeine standard 1000ppm from the caffeine standard.
    b. From 1000ppm, prepare serial dilution for caffeine standard: 5ppm, 15ppm, 25ppm, 50ppm
    and 100ppm with ready filter de ionize water.

    3.3 Sample preparation


    a. Obtain sample, soft drink
    b. Degas the sample by placing it in a vacuum flask and connecting the flask to vacuum pump
    or water aspirator. Leave it under vacuum until no more bubbles appears in the soda samples.
    c. Filter the degas soda through 45чL filter syringe.

    3.4 Instrument set up


    a. Refers SOP /OP/060 –HPLC operation

    4. Precautions
    • All sample must be filtered
    • Never run the column dry, make sure there is enough solvent in the reservoir.

    5. Distribution List
    1. Original – Document controller
    Malaysian Institute of Chemical
    & Bioengineering Technology

    Food Technology Department Document SOP /TP/011 Version 01


    Title Determination of Caffeine in Soft Drink by HPLC Date 17/03/08
    Page 2 of 3
    2. Copy - Analysis Laboratory Food Technology Department

    6. Appendix
    • F/SOP/TP/011

    7. Document History
    Document No & Description Description Version Date
    Issued
    Determination of Caffeine in Soft Drink by HPLC New document 01 170308

    Document End

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