Tests To Detect Adulteration in Milk

TESTS TO DETECT ADULTERATION IN MILK
There are many methods known for detection of adulteration in milk but the methods discussed
below are simple but rapid and sensitive methods to detect adulteration.
I. Detection of Neutralizers in milk
1) Rosalic acid test (Soda Test)
In milk neutralizers like hydrated lime, sodium hydroxide, sodium carbonate or sodium
bicarbonate are added which are generally prohibited.
w to detect?
ke 5 ml of milk in a test tube and add 5 ml alcohol followed by 4-5 drops of rosalic acid. If the colour of milk changes to pinkish
, then it is inferred that the milk is adulterated with sodium carbonate / sodium bicarbonate and hence unfit for human
nsumption.
s test will be effective only if the neutralizers are present in milk. If the added neutralizers are nullified by the developed
dity, then this test will be negative. In that case, the alkaline condition of the milk for the presence of soda ash has to be
imated.
w to proceed?
Take 20 ml of milk in a silica crucible and then the water is evaporated and the contents are burnt in a muffle furnace. The ash
dispersed in 10 ml distilled water and it is titrated against decinormal (N/10) hydrochloric acid using phenolphthalein as an
icator. If the
titre value exceeds 1.2 ml, then it is construed that the milk is adulterated with neutralizers.
II. Test for detection of hydrogen peroxide
Take 5 ml milk in a test tube and then add 5 drops of paraphenylene diamine and shake it well.
Change of the colour of milk to blue confirms that the milk is added with hydrogen peroxide.
III. Test for detection of formalin
Formalin (40%) is poisonous though it can preserve milk for a long time.
How to detect?
Take 10 ml of milk in test tube and 5 ml of conc. sulphuric acid is added on the sides of the test tube
with out shaking. If a violet or blue ring appears at the intersection of the two layers, then it shows
the presence of formalin.
IV. Test for detection of sugar in milk
Generally sugar is mixed in the milk to increase the solids not fat content of milk i.e. to increase the
lactometer reading of milk, which was already diluted with water.
How to detect?
Take 10 ml of milk in a test tube and add 5 ml of hydrochloric acid along with 0.1 g of resorcinol.
Then shake the test tube well and place the test tube in a boiling water bath for 5 min. Appearance
of red colour indicates the presence of added sugar in milk.
V. Test for detection of starch
Addition of starch also increases the SNF content of milk. Apart from the starch, wheat flour,
arrowroot, rice flour are also added.
How to detect?
Take 3 ml milk in a test tube and boil it thoroughly. Then milk is cooled to room temperature and
added with 2 to 3 drops of 1% iodine solution. Change of colour to blue indicates that the milk
is adulterated with starch.
VI. Test for detection of glucose
Usually poor quality glucose is added to milk to increase the lactometer reading. There are two
tests available to detect theadulteration of milk with glucose.
How to proceed?
1. Phosphomolybdic or Barford Test
Take 3 ml of milk in a test tube and add 3 ml Barford’s reagent and mix it thoroughly. Then keep it
in a boiling water bath for 3 min and then cool it for 2 min by immersing in tap water with out
disturbance. Then add 1 ml of phosphomolybdic acid and shake. If blue colour is visible, then
glucose is present in the milk sample.
2. Diacetic test
Take a strip of diacetic strip and dip it in the milk for 30 sec to 1 min. If the strip changes colour,
then it shows that the sample of milk contains glucose. If there is no change in the colour of the
strip, then glucose is absent. In this method the presence of glucose in milk can be quantified by
comparing the colour developed with the chart strip.
VII. Test for detection of urea

1. Urea is generally added in the preparation of synthetic milk to raise the SNF value.
Five ml of milk is mixed well with 5 ml paradimethyl amino benzaldehyde (16%). If the solution
turns yellow in colour, then the given sample of milk is added with urea.
2. Take 5 ml of milk in a test tube and add 0.2 ml of urease (20 mg / ml). Shake well at room
temperature and then add 0.1 ml of bromothymol blue solution (0.5%). Appearance of blue colour
after 10-15 min indicates the adulteration milk with urea.
VIII. Test for detection of ammonium sulphate
The presence of sulphate in milk increases the lactometer reading.
How to proceed?
5 ml of hot milk is taken in a test tube and added with a suitable acid for e.g. citric acid and the
whey thus separated is filtered. Collect the whey in another test tube and add 0.5 ml of 5% barium
chloride. Appearance of precipitate indicates the presence of ammonium sulphate in milk.
IX. Test for detection of salt
Addition of salt in milk is mainly resorted to with the aim of increasing the corrected lactometer
reading.
How to detect?
Five ml of silver nitrate (0.8%) is taken in a test tube and added with 2 to 3 drops of 1% potassium
dichromate and 1 ml of milk and thoroughly mixed. If the contents of the test tube turn yellow in
colour, then milk contains salt in it. If it is chocolate coloured, then the milk is free from salt.
X. Test for detection of pulverized soap

Take 10 ml of milk in a test tube and dilute it with equal quantity of hot water and then add 1 – 2
drops of phenolphthalein indicator. Development of pink colour indicates that the milk is
adulterated with soap.
XI. Detection of detergents in milk
Take 5 ml of milk in a test tube and add 0.1 ml of bromocresol purple solution. Appearance of violet
colour indicates the presence of detergent in milk. Unadulterated milk samples show a faint violet
colour.
XII. Detection of water in milk

Though the adulteration of milk with water can be checked by lactometer reading, other
adulterations too affect the lactometer reading. Hence freezing point depression, recognized by
AOAC, is usually adopted.
Percentage of water added = Normal freezing point – Observed freezing point X 100
Normal freezing point
Normal freezing point of milk is taken as –0.55°C. A tolerance level of 3% is given which is
equivalent to specifying a minimum freezing point depression for authentic milk of –0.55°C.
XIII. Detection of skim milk powder in milk

If the addition of nitric acid drop by drop in to the test milk sample results in the development of
orange colour, it indicates the milk is adulterated with skim milk powder. Samples with out skim
milk powder shows yellow colour.
XIV. Detection of vegetable fat in milk
The characteristic feature of milk is its fatty acid composition, which mainly consists of short chain
fatty acids such as butyric, caproic, caprylic acid; whereas the vegetable fats consist mainly of long
chain fatty acids and hence adulteration of vegetable fat in milk can be easily found out by
analyzing the fatty acid profile by gas chromatography.
XV. Detection of buffalo milk in cow milk
The presence of buffalo milk in cow milk is tested by Hansa test. It is based on immunological
assay. One ml of milk is diluted with 4 ml of water and then it is treated with 1 ml of antiserum. The
characteristic precipitation reaction indicates the presence of buffalo milk in the sample taken.
(The antiserum is developed by injecting buffalo milk proteins into rabbits).
XVI. Detection of benzoic and salicylic acid in milk
Five ml of milk is taken in a test tube and acidified with concentrated sulphuric acid. 0.5% ferric
chloride solution is added drop by drop and mixed well. Development of buff colour indicates
presence of benzoic acid and violet colour indicates salicylic acid.
XVII. Detection of borax and boric acid in milk
Five ml of milk is taken in a test tube to which 1 ml of concentrated hydrochloric acid is added and
mixed well. Tip of a turmeric paper is dipped into the acidified milk and it is dried in a watch glass
at 100°C or over a small flame. If the turmeric paper turns red, it indicates the presence of borax or
boric acid.
Confirmation can be made by adding a drop of ammonia solution on the turmeric paper and if the
red colour changes to green, it shows the presence of boric acid.

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TESTS TO DETECT ADULTERATION IN MILK

VII. Test for detection of urea

X. Test for detection of pulverized soap

XII. Detection of water in milk

XIII. Detection of skim milk powder in milk

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