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A05006 SBG Broth Base Final

This document provides instructions for using SBG Broth Base, a selective medium for isolating Salmonella bacteria. The medium is made by suspending dehydrated powder and sodium selenite in water, heating it to 100°C for 10 minutes without autoclaving, then distributing it into test tubes. Specimens such as feces are inoculated and incubated at 37°C for 18-24 hours. Cultures are then plated on a selective medium to confirm the presence of Salmonella. The medium inhibits certain bacteria but permits the growth of Salmonella, though some Salmonella strains may not grow well in it. The product should be stored dry and cool to prevent discoloration.

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72 views1 page

A05006 SBG Broth Base Final

This document provides instructions for using SBG Broth Base, a selective medium for isolating Salmonella bacteria. The medium is made by suspending dehydrated powder and sodium selenite in water, heating it to 100°C for 10 minutes without autoclaving, then distributing it into test tubes. Specimens such as feces are inoculated and incubated at 37°C for 18-24 hours. Cultures are then plated on a selective medium to confirm the presence of Salmonella. The medium inhibits certain bacteria but permits the growth of Salmonella, though some Salmonella strains may not grow well in it. The product should be stored dry and cool to prevent discoloration.

Uploaded by

Raul Jr Marquez
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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SBG Broth Base

(Selenite Brilliant Green Broth Base, for isolation of Salmonella)

Directions SBG Sulfa Medium is more suitable than this

Suspend 19.7 g of the dehydrated medium and 4.0 g of medium for the isolation of Salmonella from eggs
sodium selenite in 1,000 mL of distilled water, mix well and egg products.
to dissolve the medium. Avoiding excessive heating,
keep at 100 °C for 10 minutes. Do not autoclave. Storage
Distribute aseptically about 10 mL amounts in to Keep dry and cool place to prevent powder product
sterilized test tubes. Inoculate about 1 g of the and prepared solution form color fading.
specimen such as feces. Mix well and incubate at 37 °
C for 18 - 24 hours. Take a loopful of the culture and Formula
then inoculate on a plate of the selective medium. Use
sodium selenite of high quality.

Remarks
The medium inhibits the growth of Alcaligenes,
Pseudomonas, Proteus and other bacteria that grow on
the selenite medium, and permits the growth of
Salmonella.
S. typhi and S. paratyphi A do not grow well in the
medium.

HyServe GmbH & Co.KG, Hechenrainerstr. 24, 82449 Uffing, Germany; www.hyserve.com; [email protected] produced by Nissui Pharmaceuticls CO., LTD

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