Scribd Logo
Upload

Welcome to Scribd!

  • 385 views

    Answer Sheet FINAL Lipid

    Uploaded by

    Faridah Magumpara
    The document summarizes an experiment analyzing various samples (butter, lard, oils) to determine their lipid content. It provides formulas, physical properties, and results from solubility, grease spot, emulsion, and Sudan tests. The grease spot test identifies lipids as they leave translucent spots upon evaporation. The Sudan test stains lipids red as Sudan dye mixes with the hydrophobic lipids, allowing them to be identified by red globules floating in water. Overall, the experiments show that all samples contain lipids and emulsions form when lipids are mixed with water.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd

    Answer Sheet FINAL Lipid

    Uploaded by

    Faridah Magumpara
    385 views3 pages
    The document summarizes an experiment analyzing various samples (butter, lard, oils) to determine their lipid content. It provides formulas, physical properties, and results from solubility, grease spot, emulsion, and Sudan tests. The grease spot test identifies lipids as they leave translucent spots upon evaporation. The Sudan test stains lipids red as Sudan dye mixes with the hydrophobic lipids, allowing them to be identified by red globules floating in water. Overall, the experiments show that all samples contain lipids and emulsions form when lipids are mixed with water.

    Original Description:

    this is lipid

    Original Title

    Answer Sheet FINAL lipid

    Copyright

    © © All Rights Reserved

    Available Formats

    DOCX, PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    The document summarizes an experiment analyzing various samples (butter, lard, oils) to determine their lipid content. It provides formulas, physical properties, and results from solubility, grease spot, emulsion, and Sudan tests. The grease spot test identifies lipids as they leave translucent spots upon evaporation. The Sudan test stains lipids red as Sudan dye mixes with the hydrophobic lipids, allowing them to be identified by red globules floating in water. Overall, the experiments show that all samples contain lipids and emulsions form when lipids are mixed with water.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    385 views3 pages

    Answer Sheet FINAL Lipid

    Uploaded by

    Faridah Magumpara
    The document summarizes an experiment analyzing various samples (butter, lard, oils) to determine their lipid content. It provides formulas, physical properties, and results from solubility, grease spot, emulsion, and Sudan tests. The grease spot test identifies lipids as they leave translucent spots upon evaporation. The Sudan test stains lipids red as Sudan dye mixes with the hydrophobic lipids, allowing them to be identified by red globules floating in water. Overall, the experiments show that all samples contain lipids and emulsions form when lipids are mixed with water.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    of 3

    NAME ________________________________________ DATE PERFORMED __________________

    DATE SUBMITTED __________________


    SCORE __________________

    Title of Experiment: Experiment No. __________

    FORMULA OF REAGENTS AND SAMPLES:

    Butter No definite formula Lard C21H42O5

    Olive oil C18H34O2 Coconut oil C4H8NNaO2

    Castor oil C57H104O9 Sudan IV C24H20N4O

    Ethanol C2H6O Diethyl ether (C2H5)2O

    PHYSICAL PROPERTIES OF SAMPLES

    Sample Odor State Color Appearance


    Minola Coconut Oil Sweet custard-like Liquid Yellow Clear with bubbles
    Anchor Butter Milk-like Solid Yellow Soft
    Castor Oil Oil-like Liquid Light Yellow Clear with bubbles
    Olive Oil Musty oil-like Liquid Yellow Clear with bubbles
    Vegetable Lard Oil-like Solid White Cloudy

    TEST FOR PROTEINS

    SOLUBILITY TEST

    Sample H2O Diethyl Ether


    Minola Coconut Oil Legend:
    Anchor Butter (+) - Sample is soluble in reagent
    Castor Oil (–) - Sample is insoluble in
    Olive Oil reagent
    Vegetable Lard

    GREASE SPOT TEST

    Sample (+)/(-) Size of Spot (cm) Indication Appearance


    Sample is a Slightly translucent grease
    Minola Coconut Oil + 0.6 cm
    lipid spot
    Sample is a Sample stayed in the
    Anchor Butter + 0.2 cm
    lipid center
    Sample is a Most translucent grease
    Castor Oil + 0.5 cm
    lipid spot
    Sample is a Slightly translucent grease
    Olive Oil + 0.8 cm
    lipid spot
    Sample is a Opaque; Grease spot did
    Vegetable Lard + 0.15 cm
    lipid not spread
    Legend: (+) – Presence of grease spot (–) – Absence of grease spot
    EMULSION TEST

    Sample (+)/(-) Indication Appearance


    White cloudy mixture with translucent layer
    Minola Coconut Oil + Sample is a lipid
    at top
    Yellow layer present at the top with white
    Anchor Butter Sample is a lipid
    + cloudy mixture below
    Castor Oil + Sample is a lipid White cloudy layer at top
    White cloudy mixture with translucent layer
    Olive Oil + Sample is a lipid
    at top
    White cloudy mixture with translucent layer
    Vegetable Lard + Sample is a lipid
    at top
    Legend: (+) – Presence of emulsion (–) – Absence of emulsion

    SUDAN TEST

    Appearance
    Sample (+)/(-) Indication
    Upper Layer Lower Layer
    Light red clear
    Minola Coconut Oil + Sample is a lipid Red layer of stain
    solution
    Orange
    Anchor Butter + Sample is a lipid Cloudy red layer
    heterogenous layer
    Clear intense red
    Castor Oil + Sample is a lipid Red clear solution
    layer
    Darker red Red homogenous
    Sample is a lipid
    Olive Oil + homogenous layer layer
    Red layer with
    Intense red layer with
    Vegetable Lard + Sample is a lipid presence of
    presence of globules
    globules
    Legend: (+) – Presence of Sudan stain (–) – Absence of Sudan stain

    Upper layer red color intensity comparison:


    Minola Coconut Oil > Castor Oil > Vegetable Lard > Olive Oil > Anchor Butter

    Lower layer red color intensity comparison:


    Olive Oil > Castor Oil > Vegetable Lard > Anchor Butter > Minola Coconut Oil

    QUESTIONS AND ANALYSIS

    1) How can the grease spot test determine if the sample is a lipid or not?

    The grease spot test can be used to determine if the sample is a lipid or not because of
    the given fact that most lipids have high boiling points, making them non-volatile. This
    means that room temperature wouldn't be enough to evaporate them. The samples
    leave a translucent spot on the filter paper because they can't absorb enough heat from
    the air and get evaporized. Therefore, the samples that leave a translucent spot on the
    paper are non-volatile and, thus, are lipids.

    2) How can the Sudan test indicate the presence of lipid?

    Sudan is a red hydrophobic fat-soluble dye. Because of this property, when Sudan is
    placed in a solution containing lipids, it mixes with the lipids, a reaction that stains the lipid
    red. Since both lipids and Sudan are hydrophobic, when the test tube containing water, a
    lipid sample, and Sudan stain is mixed vigorously, the lipid molecules, now bonded with
    the Sudan molecules and stained red, form globules and float in the water. When this type
    of appearance is observed in a test tube after conducting the Sudan test, that test tube
    holds a lipid sample.

    You might also like