Scribd
Upload
160 views4 pages

Pex 08 04

1. This document appears to be a lab report submitted by John Michael F. Ruiz on assessing lipase digestion of fat through a simulation experiment. 2. John scored 0% on the pre-lab quiz by answering none of the questions correctly about the chemical and physical processes of lipid digestion. 3. The experiment involved 6 tubes with different combinations of lipase, vegetable oil, bile salts, and buffer solutions of varying pH levels. The pH and time were recorded but John did not provide any analysis of the results.

Uploaded by

JohnMichaelFabellaRuiz
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOC, PDF, TXT or read online on Scribd
160 views4 pages

Pex 08 04

1. This document appears to be a lab report submitted by John Michael F. Ruiz on assessing lipase digestion of fat through a simulation experiment. 2. John scored 0% on the pre-lab quiz by answering none of the questions correctly about the chemical and physical processes of lipid digestion. 3. The experiment involved 6 tubes with different combinations of lipase, vegetable oil, bile salts, and buffer solutions of varying pH levels. The pH and time were recorded but John did not provide any analysis of the results.

Uploaded by

JohnMichaelFabellaRuiz
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOC, PDF, TXT or read online on Scribd
You are on page 1/ 4

Name: John Michael F.

Ruiz

Exercise 8: Chemical and Physical Processes of Digestion: Activity 4: Assessing Lipase Digestion of Fat Lab Report

Pre-lab Quiz Results


You scored 0% by answering 0 out of 4 questions correctly.

1. Which of the following is/are true of bile?


Correct answer: b. It works by a physical process.

You have not answered this question.

2. The substrate used in this simulation is


Correct answer: c. vegetable oil.

You have not answered this question.

3. When fatty acids are liberated by lipase, the


pH Correct answer: b. decreases.

You have not answered this question.

4. One of the products of the chemical digestion of lipids is


Correct answer: d. fatty acids.

You have not answered this question.

01/22/18 page 1
Experiment Results
Predict Question:
Predict Question: Which tube do you think will have the highest lipase activity?
You did not answer this question.

Stop & Think Questions:


What does the pH measure?
You did not answer this question.
Correct answer: d. both lipase activity and fatty acid release

Why is it difficult to detect whether lipase is active in tube 5?


You did not answer this question.
Correct answer: d. The pH is already very low, so a decrease in pH might be difficult to detect.

Experiment Data:

Tube No. Reagent 1 Reagent 2 Reagent 3 Reagent 4 Time Temp. pH


1 Lipase Vegetable Oil Bile Salts pH 7.0 Buffer 60 37 6.21
2 Lipase Vegetable Oil Deionized pH 7.0 Buffer 60 37 7.00
Water
3 Lipase Deionized Water Bile Salts pH 9.0 Buffer 60 37 9.00
4 Deionized Water Vegetable Oil Bile Salts pH 7.0 Buffer 60 37 7.00
5 Lipase Vegetable Oil Bile Salts pH 2.0 Buffer 60 37 2.00
6 Lipase Vegetable Oil Bile Salts pH 9.0 Buffer 60 37 9.00

01/22/18 page 2
Post-lab Quiz Results
You scored 0% by answering 0 out of 4 questions correctly.

1. What is the product of lipase hydrolysis?


You did not answer this question.

Correct answer: d. fatty acids

2. From your results, which pH is ideal for pancreatic lipase digestion?


You did not answer this question.

Correct answer: b. pH 7.0

3. Which tube confirms that there is no lipase in bile salts or vegetable oil?
You did not answer this question.

Correct answer: c. tube 4

4. From your results, where (in theory) would pancreatic lipase be


active? You did not answer this question.

Correct answer: d. mouth and pancreas

01/22/18 page 3
Review Sheet Results
1. Explain why you can't fully test the lipase activity in tube 5.
You did not answer this question.

2. Which tube had the highest lipase activity? How well did the results compare with your prediction? Discuss possible
reasons why it may or may not have matched.
You did not answer this question.

3. Explain why pancreatic lipase would be active in both the mouth and the pancreas.
You did not answer this question.

4. Describe the process of bile emulsification of lipids and how it improves lipase activity.
You did not answer this question.

01/22/18 page 4

You might also like