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    Bone Bank

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    Md Ahsanuzzaman Pinku
    A bone bank collects, processes, sterilizes, and stores allogenic cadaver bone for future orthopedic procedures and bone grafts. Donor bone undergoes sterilization through methods like freezing, irradiation, or chemical treatment to reduce antigenicity and disease transmission risk. Processed bone is cut and shaped, then rapidly frozen and stored for reconstitution and clinical use. Freezing, freeze-drying, or demineralization can reduce the immune response and potential rejection of allograft bone.

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    Bone Bank

    Uploaded by

    Md Ahsanuzzaman Pinku
    166 views2 pages
    A bone bank collects, processes, sterilizes, and stores allogenic cadaver bone for future orthopedic procedures and bone grafts. Donor bone undergoes sterilization through methods like freezing, irradiation, or chemical treatment to reduce antigenicity and disease transmission risk. Processed bone is cut and shaped, then rapidly frozen and stored for reconstitution and clinical use. Freezing, freeze-drying, or demineralization can reduce the immune response and potential rejection of allograft bone.

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    Bone bank

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    A bone bank collects, processes, sterilizes, and stores allogenic cadaver bone for future orthopedic procedures and bone grafts. Donor bone undergoes sterilization through methods like freezing, irradiation, or chemical treatment to reduce antigenicity and disease transmission risk. Processed bone is cut and shaped, then rapidly frozen and stored for reconstitution and clinical use. Freezing, freeze-drying, or demineralization can reduce the immune response and potential rejection of allograft bone.

    Copyright:

    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    166 views2 pages

    Bone Bank

    Uploaded by

    Md Ahsanuzzaman Pinku
    A bone bank collects, processes, sterilizes, and stores allogenic cadaver bone for future orthopedic procedures and bone grafts. Donor bone undergoes sterilization through methods like freezing, irradiation, or chemical treatment to reduce antigenicity and disease transmission risk. Processed bone is cut and shaped, then rapidly frozen and stored for reconstitution and clinical use. Freezing, freeze-drying, or demineralization can reduce the immune response and potential rejection of allograft bone.

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    © All Rights Reserved
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    Bone bank

    Introduction:
    It is the laboratory where allogenic cadaver bone is collected,
    processed including sterilization and stored for future grafting

    Purpose of bone bank:


    1. To provide large amount of bone at a time
    2. To reduce the donor site morbidity
    3. To provide grafts to the diseased donor site

    Aim:
    To provide bone grafts with low antigenicity and maximum osteogenic
    potential
    Bone banking should fulfill the following components
    A. Selection of donor – donor must meet the following criteria
    a) No microbial or viral infection
    b) No malignant disease
    c) No history of administration of long acting isotopes
    d) A complete diagnostic autopsy
    i. Bacteriological culture of blood & tissue
    ii. Serological test for syphilis, HIV
    iii. Determination of Australia antigen (HBsAg)
    Minimize the risk of disease transmission
    B. Excision – can be excised and collected in 2 ways
    a) Grafts taken aseptically from a previously selected donor
    b) Removal of graft from non selected donor and sterilized
    later on

    Source of graft
     Ileum, Femur, Tibia, humerus , ribs, or vertebra
     Cortical bones are cut by air cooled saw
     Cancellous bones are cut by osteotome
    Processing
    a) The periosteum stripped off immediately
    b) Irrigate copiously with saline and processed into
    various sizes and shapes to suit different orthopaedic procedure
    c) Then they are put into sterile glass container
    and sealed aseptically & labeled
    d) Samples from each cadaver bone are taken for
    bacteriological studies.

    Storage – Bone graft are frozen rapidly to -70 – 80 degree and stored as
    a. Frozen state – for clinical use
    b. Freeze dried or
    c. Demineralized freeze dried
    Sterilization
    a. If grafts are obtained aseptically from a
    selected donor – not necessary
    b. In other cases sterilization can be done by
    i. Ionizing radiation
    ii. Treating with ethylene oxide
    iii. Chemical method – treating with merthiolate
    Freeze drying
    Freeze dried bone must be reconstituted before use – this is done by
    injecting normal saline into the container. Time require for reconstitution is
    about
     2 hours for cancellous and
     24 hours for cortical bone
    Advantage
     Can be indefinitely stored at room temperature
     Apparently reduced the antigenicity

    Immunegenicity of allograft
    Fresh allografts, though dead, are not immunologically acceptable.
    They induce an inflammatory response in the host and this may lead to
    rejection.
    However, antigenicity can be reduced by
     Freezing (at –70°C),
     Freeze-drying or
     By ionizing radiation.
     Demineralization
     it may also enhance the osteoinductive properties of
    the graft
     Acid extraction of allograft bone yields demineralized
    bone matrix,
     This contains collagen and growth factors.
     It is available in a variety of forms (putty, powder,
    granules) and is sometimes combined with other types
    of bone substitutes.
     Osteoinductive property is not so impressive
     One way to supplement the properties of
    demineralized bone matrix is to use it as an autologous
    bone graft expander.

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