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Exercise

1. There was some variation observed in the shape and size of E. coli colonies, but the number of single colonies in quadrant four was clearly observed. 2. The purpose of the zig-zag motion when streaking is to prevent E. coli colonies from overlapping and to obtain well-separated single colonies, especially in the last quadrant. 3. The inoculating loop must be resterilized between streaks to avoid transferring microorganisms between streaks and depositing the original mixture everywhere, which could prevent isolated colonies from forming.

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Shalini Muthu
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115 views2 pages

Exercise

1. There was some variation observed in the shape and size of E. coli colonies, but the number of single colonies in quadrant four was clearly observed. 2. The purpose of the zig-zag motion when streaking is to prevent E. coli colonies from overlapping and to obtain well-separated single colonies, especially in the last quadrant. 3. The inoculating loop must be resterilized between streaks to avoid transferring microorganisms between streaks and depositing the original mixture everywhere, which could prevent isolated colonies from forming.

Uploaded by

Shalini Muthu
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as DOCX, PDF, TXT or read online on Scribd
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Exercise:

1. Write your results showed any variation?

There was some variation that was identified during the observation of result. It
is not a major variation however the shape, size of E.coli colony observed were the
same. But, the number of single E.coli colony in quadrant four is observed clearly.

2. In step 7, what is the proper purpose for the zig-zag motion?

The purpose for doing the zig-zag motion is so that the bacteria E.coli doesn’t not
overlap to obtaining the single colony. Other than that, it is also to gather fewer
number of colony so the last quadrant can establish single colony E.coli.

3. Why is the inoculating loop had to be resterilised between each new streak?

The loop is sterilized by heating the loop in the blue flame of the Bunsen burner,

between streaking and thus lesser microorganisms are deposited as the streaking

progresses. Besides that, also to avoid breathing on cultures or sterile instruments.

It is also can avoid original mixture culture from mixing everywhere.

4. Ideally, why should a new streak intersect the pervious one at only single point?

Ideally, it is important to get a new streak need to intersect with the previous one
at only a single point because by doing that it can get the isolated colonies. If this
intersection occurs with more than one point then it will end up getting isolated
colonies which are not individual because as microorganism has more than one
way to grow.
5. Describe, the appearance of a single streak E.coli colony? Why can it be considered
genetically homogenous?

The appearance of a single streak E.coli colony is its shape of the colony is circular
and its elevation is raised. The E.coli colony appeared smooth and shiny and there
is no pigmentation which is it had no colour. The sizes of the colony were
punctiform and some were small. The colony were opaque whereby the colony
cannot be seen through and the colony also entirely margined. E.coli is considered
to be genetically homogenous because when the colony is completely isolated
from other colonies, it is considered genetically dinstinct or purfe because the
colony arises from a single cell after many divisions. So, the same DNA was shared
by cells in the colony at original cells.

Mid-log suspension culture

1. What variables influence the length of time for E.Coli culture to reach mid-log phase?

The variables that influence the length of time for an E.coli culture to reach mid-
log phase is the incubation and agitation.

2. What are the disadvantages of beginning a mid-log culture from colony scraped off a plate
as opposed to an inoculum of overnight culture?

The disadvantage of beginning a mid-log phase culture from colony scraped off
agar compare to an inoculum of overnight culture are the colony which are scraped
off a plate will be direct transfer without agitation which does not provide a proper
aeration and nutrient exchange while an overnight culture will be incubated with
a continuous aeration which provide a rapid growth of colony.

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