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To prepare 10mL of a 0.02M acetate buffer at pH 4.30, one would need 1mL of 0.05M NaOH and 4mL of 0.05M acetic acid. Using the Henderson-Hasselbalch equation, the document calculates the molar ratios and volumes of each stock solution required. It then explains that the total moles of acetic acid needed accounts for generating the conjugate base during the reaction between the weak acid and strong base used to make the buffer.

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32 views1 page

Assignment Guide

To prepare 10mL of a 0.02M acetate buffer at pH 4.30, one would need 1mL of 0.05M NaOH and 4mL of 0.05M acetic acid. Using the Henderson-Hasselbalch equation, the document calculates the molar ratios and volumes of each stock solution required. It then explains that the total moles of acetic acid needed accounts for generating the conjugate base during the reaction between the weak acid and strong base used to make the buffer.

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That Keychain
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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How would you prepare 10mL of a 0.02M acetate buffer, pH 4.

30, from stock solutions


of 0.05M acetic acid (HAc) and 0.05M NaOH? pKa acetic acid = 4.76.

1. Use the Henderson Hasselbalch equation to find the ratio of A- to HA.


pH = pKa + log [A-] / [HA]
4.30 = 4.76 + log [A-] / [HA]
-0.46 = log [A-] / [HA]
0.34673685 = [A-] / [HA]
2. Calculate the decimal fraction (part/whole) of each buffer component.
A- = 0.34673685 / (1.00 + 0.34673685) = 0.34673685 / 1.34673685 =
0.257464441
HA = 1.00 / 1.34673685 = 0.742535559
3. Find the molarity (M) of each component in the buffer by simply multiplying the
molarity of the buffer by the decimal fraction of each component.
MA- = 0.02M x 0. 257464441= 0.005149289 = 5.15 x 10-3 M
MHA = 0.02M x 0. 742535559= 0.014850711 = 1.49 x 10-2 M
4. Calculate the moles of each component in the buffer. Moles = Molarity x Liters
of buffer
molesA- = 0.005149289M x 0.01L = 5.14929 x 10-5 moles
molesHA = 0.014850711M x 0.01L = 1.485071.47 x 10-4 moles

5. Note: Since this buffer is prepared by the reaction of a weak acid (HAc) with a
strong base (NaOH), you must determine the total moles of the weak acid
component needed because the conjugate base is made in situ.
Total moles = 5.15 x 10-5moles NaOH + 1.49 x 10-4 moles HAc = 2.00 x 10-4
moles HAc. This sum indicates that, although in the buffer one only needs 1.49 x 10-4 moles
HA, an additional 5.15 x 10-5moles is needed to generate the conjugate base in situ.
6. Calculate the volume of each stock solution required to make the buffer
Liters of stock = Moles of the buffer component / Molarity of the stock
LA- = 5.14929 x 10-5 moles / 0.05 M = 1.03 x 10-3L NaOH = 1.0mL
LHA = 2.00 x 10-4 moles / 0.05 M = 4.00 x 10-3 L CH3COOH = 4.0mL
7. To prepare this buffer, one would use appropriately sized pipets or cylinders to
measure and transfer each component to a 10 mL volumetric flask and bring the
solution to volume with H2O.

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