This document contains two kinetics practice problems involving enzyme inhibition. The first problem involves determining the kinetic parameters Km and Vmax from initial rate data, and identifying the type of inhibitor. The second problem involves determining the kinetic parameters and type of inhibitors A and B using Michaelis-Menten and Lineweaver-Burk plots with rate data in the absence and presence of inhibitors.
This document contains two kinetics practice problems involving enzyme inhibition. The first problem involves determining the kinetic parameters Km and Vmax from initial rate data, and identifying the type of inhibitor. The second problem involves determining the kinetic parameters and type of inhibitors A and B using Michaelis-Menten and Lineweaver-Burk plots with rate data in the absence and presence of inhibitors.
This document contains two kinetics practice problems involving enzyme inhibition. The first problem involves determining the kinetic parameters Km and Vmax from initial rate data, and identifying the type of inhibitor. The second problem involves determining the kinetic parameters and type of inhibitors A and B using Michaelis-Menten and Lineweaver-Burk plots with rate data in the absence and presence of inhibitors.
This document contains two kinetics practice problems involving enzyme inhibition. The first problem involves determining the kinetic parameters Km and Vmax from initial rate data, and identifying the type of inhibitor. The second problem involves determining the kinetic parameters and type of inhibitors A and B using Michaelis-Menten and Lineweaver-Burk plots with rate data in the absence and presence of inhibitors.
1. Consider the following set of data and answer the following questions:
[S] (M) V (umol/min) V (+ inhibitor) (umol/min)
-6 6 x 10 20.8 12 -5 1 x 10 29 15 -5 2 x 10 45 20 -5 6 x 10 67.6 24 -4 1.8 x 10 87 28
a. Plot the data on a Lineweaver-Burk plot (be sure to label axes)
b. Determine the Km c. Determine the Vmax d. The second set of velocities represents the rate of the reaction when an inhibitor is added. Plot these data on the same graph as above and determine the new Km and Vmax and the type of inhibitor (competitive, uncompetitive, non-competitive). e. Can the effects of the inhibitor be over-ridden by adding more substrate? Why?
2. The kinetics of an enzyme were analyzed in the absence of inhibitors, as
well as in the presence of Inhibitor A and Inhibitor B. Using the given data below, construct or calculate the following (Make sure to label graphs with appropriate axes and equations, and circle final answers):
a. Write out the Michaelis-Menten and Lineweaver-Burke equations
b. Plot all of the data on one plot as Michaelis-Menten saturation curves c. Plot all of the data on one plot in the Lineweaver-Burk format d. Define Km and Vmax e. Mathematically determine the Km and Vmax in the absence and in the presence of inhibitors f. Determine what type of inhibitors A and B are
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