Scribd
Upload
30 views4 pages

1/R0 Vs 1/ (Pyruvate) For Different (Oxamate)

The document contains data from experiments measuring the initial rate (1/R0) of an enzymatic reaction with varying concentrations of substrate (1/[pyruvate]) and inhibitor (Oxamate). Linear regression lines are fitted to the data sets for different Oxamate concentrations. Higher Oxamate concentrations result in lower reaction rates and shallower slopes, indicating Oxamate is a competitive inhibitor that binds the enzyme's active site.

Uploaded by

John Lane
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as XLSX, PDF, TXT or read online on Scribd
30 views4 pages

1/R0 Vs 1/ (Pyruvate) For Different (Oxamate)

The document contains data from experiments measuring the initial rate (1/R0) of an enzymatic reaction with varying concentrations of substrate (1/[pyruvate]) and inhibitor (Oxamate). Linear regression lines are fitted to the data sets for different Oxamate concentrations. Higher Oxamate concentrations result in lower reaction rates and shallower slopes, indicating Oxamate is a competitive inhibitor that binds the enzyme's active site.

Uploaded by

John Lane
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as XLSX, PDF, TXT or read online on Scribd
You are on page 1/ 4

1/pyru 1/r 1/R0 vs 1/[pyruvate] for

250 34.60208
300
4 -0.0289 1041.667 37.59398
250 f(x) = 0.0507282063x + 16.39027
0.96 -0.0266 2631.579 68.02721
0.38 -0.0147 4166.667 111.9821 200 f(x) = 0.0400019009x + 13.80369
0.24 -0.00893 5000 119.7605 150

1/R0
0.2 -0.00835
100 f(x) = 0.0196987097x + 22.82229

50

0
0 1000 2000 3000 4000 5000 6000
1/[pyruvate]

1/R0 vs 1/[pyru
250

1/pyru 1/r 200 f(x) = 0.0400019009x + 13


250 33.22259
150
4 -0.0301 1041.667 53.8503

1/R0
0.96 -0.01857 2631.579 108.6957 100
0.38 -0.0092 4166.667 163.9882
0.24 -0.006098 5000 232.8831 50

0.2 -0.004294 0
0 1000 2000 3000 400
1/[pyruvate]

1/R0 vs 1/[pyr
300

250 f(x) = 0.05072820

1/pyru 1/r 200


250 35.08772 150
1/R0

4 -0.0285 1041.667 66.22517


100
0.96 -0.0151 2631.579 140
0.38 -0.0198 4166.667 235.8491 50

0.24 -0.00424 5000 268.8172 0


0 1000 2000 30
0.2 -0.00372
1/[pyru
0
0 1000 2000 30
1/[pyru
vs 1/[pyruvate] for different [Oxamate]

= 0.0507282063x + 16.3902749037
0mM [Oxamate]
= 0.0400019009x + 13.8036903761 Li near (0mM [Oxamate])
0.2mM [Oxa mate]
Li near (0.2mM [Oxamate])
= 0.0196987097x + 22.8222904896 0.4 mM [Oxamate]
Li near (0.4 mM [Oxa mate])

2000 3000 4000 5000 6000


1/[pyruvate]

1/R0 vs 1/[pyruvate] for 0.2mM [Oxamate]

f(x) = 0.0400019009x + 13.8036903761

Col umn E
Li near (Col umn E)

1000 2000 3000 4000 5000 6000


1/[pyruvate]

1/R0 vs 1/[pyruvate] for 0.4mM [Oxamate]


300

250 f(x) = 0.0507282063x + 16.3902749037

200
Col umn E
150
Linea r (Col umn E)
100

50

0
0 1000 2000 3000 4000 5000 6000
1/[pyruvate]
0
0 1000 2000 3000 4000 5000 6000
1/[pyruvate]

You might also like