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Dissolution: How To Calculate Dissolution Calculation in Excel Sheet

The document describes the process for calculating drug dissolution using Excel. It involves: 1) Creating a standard calibration curve by measuring the absorbance of drug solutions of known concentrations; 2) Plotting the calibration curve as a scatter graph in Excel and determining the equation; 3) Using the equation to calculate concentrations of test solutions based on their absorbance readings over time; and 4) Calculating dissolution parameters like cumulative drug release percentage to assess drug release.

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Sagar kishor savale
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© © All Rights Reserved
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Download as PDF, TXT or read online on Scribd
1K views

Dissolution: How To Calculate Dissolution Calculation in Excel Sheet

The document describes the process for calculating drug dissolution using Excel. It involves: 1) Creating a standard calibration curve by measuring the absorbance of drug solutions of known concentrations; 2) Plotting the calibration curve as a scatter graph in Excel and determining the equation; 3) Using the equation to calculate concentrations of test solutions based on their absorbance readings over time; and 4) Calculating dissolution parameters like cumulative drug release percentage to assess drug release.

Uploaded by

Sagar kishor savale
Copyright
© © All Rights Reserved
Available Formats
Download as PDF, TXT or read online on Scribd
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Dissolution How to Calculate Dissolution Calculation in Excel Sheet

Dissolution: How to Calculate Dissolution Calculation in Excel Sheet

Step 1: Std. Calibrating curve

10 mg drug was dissolved in 100 ml pH 6.8 Phosphate Buffer, to prepared 100 ug/ml solution

0.2 ml solution is diluted with 10 ml Buffer 2 g/ml solution

0.4 ml solution is diluted with 10 ml Buffer 4 g/ml solution

0.6 ml solution is diluted with 10 ml water 6 g/ml solution

0.8 ml solution is diluted with 10 ml Buffer 8 g/ml solution

1.0 ml solution is diluted with 10 ml Buffer 10 g/ml solution

1.2 ml solution is diluted with 10 ml Buffer 12 g/ml solution

(Above all solutions are 10 g/ml solution)

Step 2: Take absorbance of all solution and Plot graph in MS Excel.

Step 3: After taking absorbance, to plot graph in Excel, Click Insert Chart Scatter

Select the Graph

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Dissolution How to Calculate Dissolution Calculation in Excel Sheet
Inserts

Scatter
Chart

Select Graph

Absorbance of Prepared
Concentrations (10 g/ml)

Generated Graph

Step 3: To give Chart Tile or Axis Tile

Click here to give chart title and Axis title

Title

Axis Title

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Dissolution How to Calculate Dissolution Calculation in Excel Sheet

Step 4: Generate the equation

To select line of the graph write click click Add Trendline

To click Format Trendline

Tic Display equation on chart

Tic Display R Squared value on chart

Generate Equation

Y = mx + c
Where, Y = Absorbance,
m= Slope or gradient,
x = Concentration,
c = Y intercept,

Write click

To select line of graph

Add Trendline

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Dissolution How to Calculate Dissolution Calculation in Excel Sheet

To click Format Trendline


This winow is open at write side of the

computer screen

Tic Display equation on chart

Tic Dispay R squared on chart

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Dissolution How to Calculate Dissolution Calculation in Excel Sheet

This winow is open at write side of the

computer screen

Generate this equation

Step 5: To Prepared Dissolution sheet

Conc Conc Conc Conc Conc


Time Abs DF CDR %CDR
g/ml g/ml mg/ml mg/5ml mg/900ml

0 0 0 0 0 0 0 0 0 0

First Reading is always zero


Time: Time for dissolution is vary dosage from to dosage from.
Abs.: Taking solution after suitable time interval, to take absorbance of this solution, if absorbance is
more than 1, to dilute the solution.

Conc g/ml: 1. To calculate Conc g/ml with the help of Y = mx + c (Equation: std.
calibrating curve) this equation,
2. To convert this equation in x = Y-c / m (Y = Abs.)
DF: dilution factor is indicate number time to dilute the given solution (10, 100 and 1000).
Conc g/ml: DF * Conc g/ml to get Conc g/ml.

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Dissolution How to Calculate Dissolution Calculation in Excel Sheet

Conc mg/ml: Conc g/ml / 1000 to get Conc mg/ml.


Conc mg/5ml: Conc mg/ml * 5 to get Conc mg/5ml.
Conc mg/900ml: Conc mg/ml * 900 to get Conc mg/900ml.
CDR (cumulative drug release): to select reading in below the zero in CDR column *
First reading 0 in Conc mg/900ml to get CDR.
%CDR: (% cumulative drug release): (CDR*100) / Labelled Claim of drug to get
%CDR.

IMPORTANT NOTE:

1. In every calculation in Excel sheet, Always starting the calculation with = this sine
(equal to sine), if youre not used this sine calculation was not done.

Always starting calculation with = this sine

2. After complete all calculation to press ENTER button to give your reading.

Mr. Sagar Kishor Savale


[Department of Pharmaceutics]
[R. C. Patel Institute of Pharmaceutical Education and Research Shirpur, 425405, MS, India]
[email protected]

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