Products For Microbiological Control
Products For Microbiological Control
Products For Microbiological Control
Microbiological Control
2 Microbiological Analysis
Benefit from Sartorius long-standing expertise in microbiological quality control while increasing
performance and enhancing your workflows. To help improve the safety of quality-critical processes,
we offer premium products for microbial limit testing, sterility testing, air monitoring and
Mycoplasma detection. Obtain reproducible,
Content reliable results for in-process and final release testing
to meet the industrys requirements of today and anticipate the demands of tomorrow.
Table of Contents
Microbiological Enumeration
7 Membrane Filters
18 Culture Media
28 Filtration Units
40 Filtration Equipment
Air Monitoring
101 Gelatine Membrane Filters (GMF), also DNA-free
102 BACTair
103 MD8 airscan
105 AirPort MD8
106 Accessories
110 EXTEND Instrument Services
Chemical Compatibility
114 Filter Materials
116 Filter Holder|O-ring Materials
118 Index
Intro Microbiological Enumeration 5
Microbiological Enumeration
Table of Contents
Membrane Filters
7 Gridded Cellulose Nitrate Membrane Filters
(Cellulose Mixed Ester)
12 Microsart e.motion Membrane Filters
13 Microsart e.motion Dispenser
14 Ungridded Cellulose Nitrate and Cellulose Acetate
Membrane Filters
16 Hydrophobic Edged Membrane Filters
Culture Media
19 Microsart @media
21 Nutrient Pad Sets
26 Culture Media in Bottles and Tubes
Filtration Units
29 Biosart 100 Monitors
32 Biosart 100 Nutrient Media
33 Microsart Funnel 100 | Microsart Funnel 250
35 Microsart @filter 100 | Microsart @filter 250
38 Biosart 250 Funnels
Filtration Equipment
41 Combisart The Sterile-Vented Filter Station
47 Microsart Combi.jet
49 How to Setup a Vacuum Filtration System
49 Traditional Multi-Branch Manifolds and Individual Filter Holders
52 Accessories for Vacuum Filter Holders and Manifold Systems
58 Electric Vacuum Pumps
6 Microbiological Enumeration Intro
Specifications
Design 7 or 50 mm in diameter, white, grey or green
4
and gridded
Growth Promotion Test No enhancement or inhibition by the grid lines
acc. to ISO 7704 No enhancement or inhibition due to chemical
extractables
No enhancement or inhibition by the sterilization
process
Sterility test Sterile
Thermal resistance 130C max.
Thickness acc. to DIN 53105 115145 m
Chemical compatibility Aqueous solutions (pH 4-8), hydrocarbons and several
other organic solvents. Detailed information in section
Chemical Compatibility under Cellulose Nitrate type 113
(page 113).
Ordering Information
White Membrane with Black Grid, for Detection of Bacteria with Dyed Media,
Particle Count and Microscopy, Type 114, Individually, Sterile Packaged
Pore Size Diameter Pack Size Order No.
0.2 m 47 mm 100 11407--47----ACN
47 mm 1,000 11407--47----ACR
50 mm 100 11407--50----ACN
50 mm 1,000 11407--50----ACR
0.45 m 47 mm 100 11406--47----ACN
47 mm 1,000 11406--47----ACR
50 mm 100 11406--50----ACN
50 mm 1,000 11406--50----ACR
0.45 m High-Flow* 47 mm 100 114H6--47----ACN
47 mm 1,000 114H6--47----ACR
50 mm 100 114H6--50----ACN
50 mm 1,000 114H6--50----ACR
0.65 m 47 mm 100 11405--47----ACN
50 mm 100 11405--50----ACN
0.8 m 47 mm 100 11404--47----ACN
47 mm 1,000 11404--47----ACR
50 mm 100 11404--50----ACN
1.2 m 47 mm 100 1
1403--47----ACN
47 mm 1,000 11403--47----ACR
50 mm 100 11403--50----ACN
50 mm 1,000 11403--50----ACR
White Membrane with Green Grid, for Detection of Bacteria with Dyed Media,
Particle Count and Microscopy, Type 139, Individually, Sterile Packaged
0.45 m 47 mm 100 13906--47----ACN
47 mm 1,000 13906--47----ACR
50 mm 100 13906--50----ACN
50 mm 1,000 13906--50----ACR
0.45 m High-Flow* 47 mm 100 139H6--47----ACN
47 mm 1,000 139H6--47----ACR
50 mm 100 139H6--50----ACN
0.65 m 47 mm 100 13905--47----ACN
1.2 m 47 mm 100 13903--47----ACN
Green Membrane with Dark-Green Grid, Providing Optimal Contrast to Light-
Colored or Transparent Bacteria Colonies, Type 138, Individually, Sterile Packaged
0.45 m 47 mm 100 13806--47----ACN
47 mm 1,000 13806--47----ACR
50 mm 100 13806--50----ACN
50 mm 1,000 13806--50----ACR
Grey Membrane (After Wetting Black) with White Grid, for Detection of Yeasts
and Molds, Particle Count and Microscopy, Type 130, Individually, Sterile Packaged
0.45 m 47 mm 100 13006--47----ACN
47 mm 1,000 13006--47----ACR
50 mm 100 13006--50----ACN
50 mm 1,000 13006--50----ACR
0.45 m High-Flow 47 mm 100 130H6--47----ACN
47 mm 1,000 130H6--47----ACR
50 mm 100 130H6--50----ACN
50 mm 1,000 130H6--50----ACR
0.65 m 47 mm 100 13005--47----ACN
50 mm 100 13005--50----ACN
50 mm 1,000 13005--50----ACR
0.8 m 47 mm 100 13004--47----ACN
47 mm 1,000 13004--47----ACR
50 mm 100 13004--50----ACN
10 Microbiological Enumeration Membrane Filters
Specifications
Design 25, 47 or 50 mm in diameter, white,
grey or green and gridded
Growth Promotion Test No enhancement or inhibition by the grid lines
acc. to ISO 7704 No enhancement or inhibition due to chemical
extractables
Thermal resistance 130C max.
Thickness acc. to DIN 53105 115145 m
Chemical compatibility Aqueous solutions (pH 4-8), hydrocarbons and several
other organic solvents. Detailed information in section
Chemical Compatibility under Cellulose Nitrate type 113
(page 113).
Ordering Information
White Membrane with Black Grid, for Detection of Bacteria with Dyed Media,
Particle Count and Microscopy, Type 114, Non-Sterile
Pore Size Diameter Pack Size Order No.
0.2 m 25 mm 100 11407--25------N
47 mm 100 11407--47------N
47 mm 1,000 11407--47------R
50 mm 100 11407--50------N
0.45 m 25 mm 100 11406--25------N
47 mm 100 11406--47------N
47 mm 1,000 11406--47------R
50 mm 100 11406--50------N
50 mm 1,000 11406--50------R
0.65 m 47 mm 100 11405--47------N
0.8 m 25 mm 100 11404--25------N
47 mm 100 11404--47------N
50 mm 100 11404--50------N
1.2 m 25 mm 100 11403--25------N
47 mm 100 11403--47------N
50 mm 100 11403--50------N
White Membrane with Green Grid, for Detection of Bacteria with Dyed Media,
Particle Count and Microscopy, Type 139, Non-Sterile
0.45 m 47 mm 100 13906--47------N
47 mm 1,000 13906--47------R
50 mm 100 13906--50------N
50 mm 1,000 13906--50------R
Grey Membrane (After Wetting Black) with White Grid, for Detection of Yeasts
and Molds, Particle Count and Microscopy, Type 130, Non-Sterile
0.45 m 25 mm 100 13006--25------N
47 mm 100 13006--47------N
47 mm 1,000 13006--47------R
50 mm 100 13006--50------N
0.65 m 47 mm 100 13005--47------N
50 mm 100 13005--50------N
0.8 m 47 mm 100 13004--47------N
50 mm 100 13004--50------N
12 Microbiological Enumeration Membrane Filters
Specifications
Please refer to the membrane type: Cellulose nitrate (cellulose ester),
gridded, individually, sterile packaged. See page 8
Ordering Information
Order Numbers for Microsart e.motion Membrane Filters
Diameter 47 mm or 50 mm, in Pack of 3 +100 Membranes, Individually,
Sterile Packaged, Without Protective Paper
Membrane Filter Pore Size Order No.
Color |Grid Color
White|black 0.2 m 11407Z-47----SCM
White|black 0.2 m 11407Z-50----SCM
White|black 0.45 m High-Flow 114H6Z-47----SCM
White|black 0.45 m High-Flow 114H6Z-50----SCM
White|black 0.45 m 11406Z-47----SCM
White|black 0.45 m 11406Z-50----SCM
White|black 0.8 m 11404Z-47----SCM
White|black 1.2 m 11403Z-47----SCM
White|black 1.2 m 11403Z-50----SCM
White|black 3 m 11402Z-47----SCM
White|green 0.45 m High-Flow 139H6Z-47----SCM
White|green 0.45 m 13906Z-47----SCM
White|green 0.45 m 13906Z-50----SCM
Green |dark green 0.45 m 13806Z-47----SCM
Green |dark green 0.45 m 13806Z-50----SCM
Grey*|white 0.45 m High-Flow 130H6Z-47----SCM
Grey*|white 0.45 m High-Flow 130H6Z-50----SCM
Grey*|white 0.45 m 13006Z-47----SCM
Grey*|white 0.45 m 13006Z-50----SCM
Grey*|white 0.65 m 13005Z-47----SCM
Grey*|white 0.65 m 13005Z-50----SCM
Grey*|white 0.8 m 13004Z-47----SCM
Grey*|white 0.8 m 13004Z-50----SCM
Specifications
Dimensions (L +H+ W) in mm 204+ 213+165
Weight 2.9 kg
Operating voltage 110 V/230 V optional
Frequency 5060 Hz
Max. power Consumption 10 W
Dispensing speed 0.5 sec
Dispenser delay 5 sec
Certificates CE Mark and EMC Directive,
European Standards EN 50081-1 and 2,
EN 50082-1 and -2, EN 61010
Ordering Information
Description Order No.
Microsart e.motion dispenser, 16712
fully automated membrane filter dispenser
Foot switch for Microsart e.motion dispenser 1ZE---0028
14 Microbiological Enumeration Membrane Filters
Specifications
Design 25, 47 or 50 mm in diameter, white
Growth Promotion Test No enhancement or inhibition by the
acc. to ISO 7704 sterilization process
No enhancement or inhibition due
to chemical extractables
Sterility test Sterile
Thermal resistance CN: 130C max. | CA: 180C max.
Thickness acc. to DIN 53105 CN: 115145 m | CA: 120 m (average value)
Chemical compatibility Aqueous solutions (pH 48), hydrocarbons and several
other organic solvents. Detailed information in section
Chemical Compatibility under Cellulose Nitrate type 113
and Cellulose Acetate type 111 (page 113).
Ordering Information
Cellulose Nitrate Membrane Filters, White, for Colony Counting, Sterility Testing,
Particle Count and Microscopy, Type 113, Individually, Sterile Packaged
Cellulose Acetate* Membrane Filters, White, for Colony Counting, Sterility Testing,
Particle Count and Microscopy, Type 111, Individually, Sterile Packaged
0.2 m 47 mm 100 11107--47----ACN
50 mm 100 11107--50----ACN
0.45 m 47 mm 100 11106--47----ACN
50 mm 100 11106--50----ACN
Specifications
Design 5, 47 or 50 mm in diameter, white or
2
white with black grid
Growth Promotion Test No enhancement or inhibition by the grid lines
acc. to ISO 7704 No enhancement or inhibition due to chemical
extractables
No enhancement or inhibition by the sterilization
process
Sterility test Sterile
Thermal resistance CN: 130C max. | CA and RC: 180C max.
Thickness acc. to DIN 53105 CN: 115145 m | CA: 120 m (average value) |
RC: 160200 m
Chemical compatibility Aqueous solutions (pH 48), hydrocarbons and
several other organic solvents, RC is resistant to almost
all solvents and is compatible in a pH-range of 3-12.
Detailed information in section Chemical Compatibility
under Cellulose Nitrate type 113, page 113, Cellulose
Acetate type 111 and Regenerated Cellulose type 184.
Membrane Filters Microbiological Enumeration 17
Ordering Information
Cellulose Nitrate Membrane Filters, White with Black Grid,
for Colony Counting and Sterility Testing, Type 131, Pack Size 100
Microsart @media
Advanced System for Agar and Membrane Transfer
Ordering Information
Media Type Target Typical Incubation Time Order No.
Microorganisms and Temperature
TSA (Tryptic Total Count 48 to 72 hrs (USP) or 14313--47----ACN
Soy Agar) 1 to 5 days (EP)
at 30 to 35C
SDA (Sabouraud Yeasts and molds 5 to 7 days at 20 to 25C 14314--47----ACN
Dextrose)
R2A Total Count 5 to 7 days at 20 to 28C 14322--47----ACN
TSA (Tryptic Soy Total Count 48 to 72 hrs (USP) or 14315--47----ACN
Agar) with Lecithin 1 to 5 days (EP)
& Polysorbate at 30 to 35C
SDA (Sabouraud Yeasts and molds 5 to 7 days at 20 to 25C 14316--47----ACN
Dextrose Agar)
with Lecithin,
Polysorbate 80
& Chloramphenicol
PCA (Plate Count Water, Waste 48 to 72 hrs at 32 to 35C 14317--47----ACN
Agar) Water and
dairy products
Accessories
Description Order No.
Microsart e.jet Transfer Pump 166MP-4
Combi.jet manifold 16848-CJ
Microsart Base 47 mm 1ZU---0002
Silicone Pressure Tubing, pressure sided, 1 meter lengths 1ZAS--0007
Silicone tubing pressure-sided, 2 m 1ZAS--0019
Silicone tubing pressure-sided, 10 m 1ZAS--0020
Minisart SRP25 vent filters 17575--------ACK
Culture Media Microbiological Enumeration 21
Ordering Information
NPS Type (Filter Type)1 Determination of Order No.2
1) The membrane filters are selected for optimum growth, together with the
corresponding nutrient media. The supplied membrane filter type is listed
within brackets:
(1) = Green with dark-green grid, 0.45 m pore size
(2) = White with green grid, 0.45 m pore size
(3) = Grey (after wetting black) with white grid, 0.65 m pore size
(4) = White with green grid, 0.65 m pore size
(5) = White with green grid, 1.2 m pore size
(6) = Grey (after wetting black) with white grid, 0.8 m pore size
(7) = White with black grid, 0.45 m pore size
(8) = Grey (after wetting black) with white grid, 0.45 m pore size
(9) = White with green grid, 0.45 m pore size, High-Flow (ideal for E. coli)
(10) = Grey (after wetting black) with white grid, 0.45 m pore size, High-Flow
Other NPS types and NPS with Microsart e.motion Membrane Filters on request.
Culture Media Microbiological Enumeration 25
Mixed culture from water E. coli colonies fluoresence in UV light E. coli and coliforms from river water E. coli and coliforms from river water E. coli and coliforms from waste water E. coli and coliforms from waste water E. coli and coliforms from waste water
Total colony count Caso, R2A
Yeasts and molds, Candida albicans Sabouraud
Other faecal bacteria Non-faecal, pathogenic bacteria Yeasts and molds Soft drinks, Acid-tolerant microorganisms, Orange Serum, VLB-S-7-S
concentrates Lactic-acid bacteria
Azide Bismuth Sulfite Cetrimide Chapman Lysine Malt Extract Sabouraud
Enterobacteria, E. coli and coliforms Endo, MacConkey
Mesophilic slime-forming bacteria, Weman
Leuconostoc
Total colony count Standard*, Standard TTC*,
TGE | Tryptone Glucose Extract
Yeasts and molds Malt Extract, m Green yeast and mold |
Schaufus Pottinger, Wallerstein Nutrient, Wort
Sugar, E. coli and coliforms Endo
Enterococcus faecalis Salmonella typhosa, streak Pseudomonas aeruginosa Staphylococcus aureus Torulopsis spec. Saccaromyces cerevisiae Alternaria humicola Torula lipolytica
sugar products Mesophilic slime-forming bacteria, Weman
Leuconostoc
Thermophilic spore formers and Glucose Tryptone
mesophilic bacteria
Yeasts and molds Malt Extract*, Schaufus Pottinger |
m Green yeast and mold, Wort*
Water Acid-tolerant microorganisms, Orange Serum
(general Lactic-acid bacteria
quality), Enterobacteria, E. coli and coliforms CHROMOCULT **, ECD, Endo, (MacConkey),
Enterococci from waste water Salmonellae from waste water Mixed culture with Pseudomonas aeruginosa Mixed culture of staphylococci Wild yeasts from lager beer Mixed culture from Saccaromyces and Rhodutorula Yeasts and molds from cough syrup Mixed culture from a soft drink mineral water, m FC, Teepol | Lauryl Sulphate, Tergitol TTC
natural water,
waste water Enterococci, Enterococcus faecalis Azide | KF Strep
Yeasts and molds Pseudomonas aeruginosa Cetrimide
Wallerstein (WL Nutrient) Glucose Tryptone Jus de Tomate Orange Serum VLB-S7-S Weman Salmonellae Bismuth Sulfite
Staphylococci, Staphylococcus aureus Chapman
Total colony count Caso, R2A, Standard, Standard TTC, TGE | Tryptone
Glucose Extract, Yeast Extract
Yeasts and molds, Candida albicans Sabouraud
Wine Acetobacter Orange Serum, Wort
(both wetted with 5-8% ethanol)
Acid-tolerant microorganisms, Orange Serum
Saccharomyces cerevisiae Saccharomyces cerevisiae Bacillus coagulans, the flat sour colony Lactic-acid bacteria, streak Rhodotorula spec. Lactobacillus brevis Leuconostoc mesenteroides
Lactic-acid bacteria
Oenococcus and other wine Jus de Tomate | Tomato Juice
* These NPS types are suitable for the determination of the mentioned microorganisms, although the media are not explicit declared in references.
The description of the typical results or any pictures show typical appearance of the mentioned microorganisms.
Lactobacillus plantarum Yeasts and molds from spoiled beer Mixed culture from canned vegetables Oenococcus oeni from wine Mixed culture from a soft drink Lactobacilli and pediococci from sediment, streak Mixed culture from sugar syrup In particular cases, color and shape of the colonies could vary from the expected habitus. Further tests may be necessary
to validate the result. Sartorius Stedim Biotech shall not be liable for consequential and | or incidental damage sustained
by any customer from the use of its products.
Nutrient Pad Sets (NPS) are subject to continuous product improvement as part of our product development program
to align our products with changing application requirements. For current specifications and lot release criteria please visit
our homepage under: www.sartorius-stedim.com/NPSSearch.
** Trade mark owner and manufacturer is Merck KGaA
Ordering Information
Agar Media in 250 ml Bottles, 4 Bottles per Box
Determination of Agar Type Order No.
Total count Nutrient 14144----------A
Yeasts and molds Wort 14157----------A
Wild yeasts Lysine 14143----------A
Lactobacilli and Pediococci and VLB-S7-S 14148----------A
other beer-spoiling organisms
Liquid Broth Media, for Drinking Water Analysis in 100 ml Bottles, 4 Bottles per Box
Determination of Media Type Order No.
Lactose fermenting Enterobacteria Lactose Broth 14155----------A
(E. coli and coliform bacteria)
Culture Media Microbiological Enumeration 27
Absorbent Pads, 47 mm, Sterile Packaged in 10 Magazines, Each with 100 Pads
Description Packaging Order No.
Absorbent Pads, 10+ 100 pads 1,000 per box, 15410--47----ALR
incl. one dispenser
Absorbent Pad Set, 10+ 100 pads 1,000 per box, 13906--47----APR
plus 1,000 membrane filters incl. two dispensers
(0.45 m, white|green)
Specifications
Housing Polystyrene
Membrane filter Cellulose nitrate (cellulose ester): choice of white,
green or grey, with grid;
Regenerated cellulose: white;
membranes removable for filing
Plug and adapter Polyethylene
Pad Cellulose
Capacity 100 ml, 10 ml graduations
Pore size 0.2 m, 0.45 m or 0.8 m
Filter diameter 47 mm
Filtration area 14.5 cm2
Max. operating pressure Vacuum only
Outlet 6.5+ 1.5 mm
Lot certificates Recovery rate, sterility and specifications
Ordering Information
Biosart 100 Monitors, 100 ml, 47 mm, Individually Packaged, Sterile, 48 Units
Pore Size Membrane Filter* Order No.
Color|Grid Color
0.2 m CN white |black 16401-47-07--ACK
0.45 m CN white |black 16401-47-06--ACK
0.45 m CN green|dark green 16402-47-06--ACK
0.45 m CN grey|white** 16403-47-06--ACK
Biosart 100 Monitors, 100 ml, 47 mm, Packaged on Trays, Sterile, 48 Units
0.2 m CN white|black 16401-47-07----K
0.45 m High-Flow CN white|black 16401-47-H6----K
0.45 m CN white|black 16401-47-06----K
0.45 m CN green|dark green 16402-47-06----K
0.45 m CN grey|white** 16403-47-06----K
0.8 m CN grey|white** 16403-47-04----K
0.45 m RC white 16404-47-06----K
Filtration Units Microbiological Enumeration 31
Each box of Biosart 100 Nutrient Media have been passed successfully. Biosart 100
contains 50 ampoules with sterile media, Nutrient Media are convenient in use and
each with 2.5 ml and a lot certificate. eliminating the handling of glass ampoules.
If stored under proper conditions (+4C),
the culture media have a shelf life of Application
12months (except for Endo, KF Strep, Microbial enumeration.
Lauryl Sulfate and Tergitol which have a
9month shelf life). Biosart 100 Nutrient Benefits
Media comply with international regula-
tions and recommendations: International Safe and Reliable
pharmacopoeias, DIN and ISO standards, Pre-sterilized media
the American Standards for Water and Certificate of Quality for every batch
Foods, mineral water regulations, guide- In compliance with international
lines of the food and beverage industries. standards
Consistent recovery
Within the scope of the quality assurance
procedure and the stringent quality control Economical
standards every batch has passed Sartorius Ready-to-use
in-house tests of growth promotion, Long shelf life
sterility, physical and technical parameters
Ordering Information
Biosart 100 Nutrient Media, 2.5 ml, Individually,
Sterile-Packaged in Ampoules, 50 Units
Media Type Determination of Order No.
Caso (acc. USP) Total count 16400-02----CA-K
R2A (acc. EP) Total count 16400-02----RA-K
TGE|Total Count Total count 16400-02----TC-K
Total Count TTC Total count 16400-02----TZ-K
m Endo E. coli and coliforms 16400-02----EN-K
m FC E. coli and coliforms 16400-02----MF-K
Lauryl Sulfate|Teepol E. coli and coliforms 16400-02----LS-K
Tergitol TTC E. coli and coliforms 16400-02----TT-K
KF Strep|Azide Enterococci 16400-02----KF-K
Cetrimide Pseudomonas aeruginosa 16400-02----CE-K
Sabouraud (acc. USP) Yeasts and molds 16400-02----SB-K
m Green yeast and mold| Yeasts and molds 16400-02----MG-K
Schaufus Pottinger
m Green yeast and mold Yeasts and molds 16400-02----GS-K
selective
Wort Yeasts and molds 16400-02----WZ-K
WL Nutrient | Yeasts and molds and bacteria 16400-02----WN-K
Wallerstein Nutrient
WL Differential | Bacteria in fermentation 16400-02----WL-K
Wallerstein Differential processes
Orange Serum Acid-tolerant microorganisms 16400-02----OS-K
MRS Lactobacilli and other soft 16400-02----MR-K
drink-spoiling microorganisms
Filtration Units Microbiological Enumeration 33
Specifications
Material Polypropylene
Capacity 100 ml, graduations at 20, 50 and 100 ml
250 ml, graduations at 50, 100, 200 and 250 ml
Filter diameter 47 mm, prefilter 40 mm (particle testing only)
Filtration area 13.2 cm2
Max. operating pressure Vacuum only
Sterilization Ethylene oxide
Lot certificate Sterility and performance test
Ordering Information
Microsart Funnel 100, Sterile Disposable Funnel, 100 ml, 100 Units
Description Order No.
Microsart Funnel 100, sterile in 5 sealed bags 16A07--10------N
Specifications
Materials Funnel: Polypropylene,
Base: ABS (Acrylnitril-Butadien-Styrol),
Membrane filter: Cellulose Nitrate (C. mixed Ester),
Regenerated Cellulose;
choice of various colours and pore sizes
Capacity 100 ml, graduations at 20, 50 and 100 ml
250 ml, 50, 100, 200 and 250 ml graduations
Filter diameter 47 mm, prefilter 40 mm (particle testing only)
Filtration area 13.2 cm2
Max. operating pressure Vacuum only
Sterilization Gamma irradiation
Lot certificate Recovery rate, sterility and performance test
Ordering Information
Microsart @filter 100, Sterile Disposable Filter Units with Lid, 47 mm, 100 ml,
Packaged on Trays, Ideal for the Use in Clean Benches, 24 Units
Pore Size Membrane Filter* Color|Grid Color Order No.
0.2 CN white|black 16D01--10-07--TG
0.45, High-Flow CN white|black 16D01--10-H6--TG
0.45, High-Flow CN grey|white** 16D03--10-H6--TG
0.45 CN green|dark green 16D02--10-06--TG
0.45 RC white (w/o grid) 16D05--10-06--TG***
0.45 CN white|black 16D01--10--06--TG
Microsart @filter 250, Sterile Disposable Filter Units with Lid, 47 mm, 250 ml,
Packaged on Trays, Ideal for the Use in Clean Benches, 16 Units
Pore Size Membrane Filter* Color|Grid Color Order No.
0.2 CN white|black 16D01--25-07--TF
0.45, High-Flow CN white|black 16D01--25-H6--TF
0.45, High-Flow CN grey|white** 16D03--25-H6--TF
0.45 CN green|dark green 16D02--25-06--TF
0.65 CN grey|white** 16D03--25-05--TF
0.45 CN white|black 16D01--25--06--TF
Filtration Units Microbiological Enumeration 37
Microsart @filter 100, Sterile Disposable Filter Units, 47 mm, 100 ml, Stacked and
Packaged in Bags, Ideal for the Use with Microsart Funnel Dispenser, 60 Units
Pore Size Membrane Filter* Color|Grid Color Order No.
0.2 CN white|black 16D01--10-07--BL
0.45, High-Flow CN white|black 16D01--10-H6--BL
0.45, High-Flow CN grey|white** 16D03--10-H6--BL
0.45 CN green|dark green 16D02--10-06--BL
0.45 RC white (w/o grid) 16D05--10-06--BL***
0.45 CN white|black 16D01--10--06--BL
Microsart @filter 250, Sterile Disposable Filter Units, 47 mm, 250 ml, Stacked and
Packaged in Bags, Ideal for the Use with Microsart Funnel Dispenser, 48 Units
Pore Size Membrane Filter* Color|Grid Color Order No.
0.2 CN white|black 16D01--25-07--BK
0.45, High-Flow CN white|black 16D01--25-H6--BK
0.45, High-Flow CN grey|white** 16D03--25-H6--BK
0.45 CN green|dark green 16D02--25-06--BK
0.65 CN grey|white** 16D03--25-05--BK
0.45 CN white|black 16D01--25--06--BK
Accessories
Description Order No.
Lids for Microsart @filter 100 and 250, Pack size: 100 1ZSF-K0007
(4 bags with 25 pieces each), gamma sterilized
Microsart Funnel Dispenser 16A08
Funnel dispenser for secure removal of single, sterile Microsart @filter packaged in bags.
In microbiological quality control, sterility sample volumes. The large inner diameter
of the equipment used for processing ensures a high-flow rate. The conical
samples is a necessary basic requirement. form allows a thorough rinsing of the
The reuseable funnels made of stainless- system subsequent to filtration. No liquid
steel or other materials which are used for is retained in the filter funnel.
membrane filtration are usually sanitized
between samples by flaming or with hot Applications
water. Both of these methods can be Microbial enumeration, particle testing
insufficiently reliable when not properly and microscopy
performed. Alternatively, the funnels could
be sterilized by autoclaving, but this is Benefits
too laborious for routine use. A disposable
sterile funnel in a certified quality is the Superior Performance
ideal solution. High-flow rate
High total throughput
Description
The Biosart 250 Funnel has been specifi- Safe and Reliable
cally designed for microbiological and Sterile or individually, sterile packaged
analytical quality assurance. Biosart 250 No risk of cross contaminations
are sterile funnels which allows for fast N o leakages due to proven closure
filtration required in the routine testing technique
of pharmaceutical and cosmetic products, No holding of hot funnels
water, food and beverages and other V isibility of the complete filtration
liquids. A Sartorius gridded membrane is
placed on a stainless-steel filter support. Economical
A Biosart 250 Funnel is simply fitted on R eady to connect and easy to use
and the sample is filtered. The funnel is Minimal amount of equipment needed
made of polypropylene and is sufficiently Autoclavable (to a limited extend)
elastic for optimal sealing with a bayonet-
type closure. Graduations are marked at
50, 100, 150, 200 and 250 ml for exact
Filtration Units Microbiological Enumeration 39
Specifications
Material Polypropylene
Capacity 250 ml, 50 ml graduations
Filter diameter 47 mm (or 50 mm), prefilter 40 mm
Filtration area 12.5 cm2
Max. operating pressure Vacuum only
Sterilization 16407--25----ALK: Ethylene oxide
16407--25----ACK: Gamma Irradiation
Lot certificates Sterility and performance tests
Ordering Information
Biosart 250 Funnels, Ready to Use Filter Funnels, 250 ml, 50 Units
Description Order No.
Biosart 250 Funnel, 50 units, individually, sterile-packaged 16407--25----ACK
Biosart 250 Funnel, 50 units, sterile-packaged 16407--25----ALK
Specifications
Stainless-steel quality High-grade stainless-steel: B.S. 304S31|AISI 304
Dimensions in mm (L|H|D) 3-branch manifold: 435|103|120
6-branch manifold: 910|103|120
Max. operating pressure Vacuum only
Sterilization By autoclaving (max. 134C),
By dry heat (max. 180C),
By flaming,
By other methods acc. to ISO 8199
Parts and materials Lid, funnel, base part, filter support, clamp and tap
made of stainless-steel. Silicone flat gasket. Silicone
lid seal
Flow rate per filter station 200 ml/min with 0.2 m membrane filter
for water at 90% vacuum 600 ml/min with 0.45 m membrane filter
Filtration area 12.5 cm2 (if using stainless-steel funnels)
Suitable membrane filter diameter 50 mm (47 mm, if using a 47 mm frit 6980103)
Outlet spout (individual system) 10 mm outer diameter
Inlet (branches only) Female thread, TR 20+ 2
Outlet (1- and 3-branches only) uick Connection Nipple DN 7
Q
(tubings with DN 10 are connectable)
Outlet (6-branch) Hose nipple DN 10
Filtration Equipment Microbiological Enumeration 43
Ordering Information
Combisart Individual System and Multi-Branch Manifolds, Made of High-Grade
Stainless-Steel, Pre-Assembled with Stainless-Steel Funnels and Lids
Description Capacity Order No.
Combisart individual filter holder, stainless-steel, 100 ml 1+ 100 ml 16219-CS
Combisart individual filter holder, stainless-steel, 500 ml 1+ 500 ml 16201-CS
Combisart 1-branch stainless-steel manifold, 100 ml 1+ 100 ml 16844-CS
Combisart 1-branch stainless-steel manifold, 500 ml 1+ 500 ml 16845-CS
Combisart 3-branch stainless-steel manifold, 100 ml 3+ 100 ml 16824-CS
Combisart 3-branch stainless-steel manifold, 500 ml 3+ 500 ml 16828-CS
Combisart 6-branch stainless-steel manifold, 100 ml 6+ 100 ml 16832-CS
Combisart 6-branch stainless-steel manifold, 500 ml 6+ 500 ml 16831-CS
6981001
stainless steel lid
6981004
funnel 40 ml 6981063
with clamp stainless steel lid
6981003
6981064 silicone lid seal
silicone lid seal
6981065
funnel 100 ml
with clamp
6980271 6981002
silicone O-ring funnel 500 ml with clamp
16840* 6980264
16 + 2 mm clamp
single base
6980264 clamp
16840*
single base
6980102 stainless steel frit 50 mm
6980103 stainless steel frit 47 mm
6980124 silicone flat gasket
6980104 PTFE flat gasket
6980270
stainless steel cap 16840* stainless steel single base
(incl. 50 mm frit and silcone gasket)
6980274 silicone O-ring 8 + 20
6980235 silicone O-ring (pack of 3)
6980267 6980225
stainless steel Plug conical
3-way tap (pack of 10) *) Alternatively to 16840 the Microsart Base 47 mm is highly recommended
for all 47 mm membrane filters, Microsart Funnels and Microsart @filter units.
Filtration Equipment Microbiological Enumeration 45
Funnels, Lids, Seals and Filter Holders to Connect on the Combisart System
Description Capacity Membrane Order No.
Filter Diameter
Stainless-steel funnel with 100 ml 47|50 mm 6981065
closure clamp
Lid, stainless-steel for 100 ml funnel 6981063
Lid seal, silicone for 100 ml funnel 6981064
Stainless-steel funnel with 500 ml 47|50 mm 6981002
closure clamp
Lid, stainless-steel for 500 ml funnel 6981001
Lid seal, silicone for 500 ml funnel 6981003
Stainless-steel funnel with 40 ml 47|50 mm 6981004
closure clamp
Polycarbonate filter holder, 250 ml 47 mm 16511
complete with filter support
and funnel
Glass filter holder, complete 30 ml 25 mm 16306
with filter support, funnel
and metal clamp
Glass filter holder, complete 250 ml 47|50 mm 16307
with filter support, funnel
and metal clamp
Microsart Combi.jet
2-Branch Stainless-Steel Manifold for Microbiological Analysis
Sterile Venting
A special feature of the Microsart
Combi.jet manifold are the stainless-steel
three-way valves (taps). They allow the
vacuum for each filter holder to be
individually controlled and each filter
station to be sterilely vented. This rules
out secondary contamination of the
underside of the filter.
48 Microbiological Enumeration Filtration Equipment
Specifications
Microsart Combi.jet
Stainless-steel quality High-grade stainless-steel: B.S. 304S31|AISI 304
Dimensions in mm (L|H|D) 246|98|130
Max. operating pressure Vacuum only
Sterilization By autoclaving (max. 134C)
Parts and materials Manifold: stainless-steel, silicone O-ring
Quick Connection Coupling PVDF, closure: stainless-steel, sealing: FKM|FPM
Inlet (manifold) Female thread, TR 20+2
Outlet Quick Connection Coupling (female),
inner diameter NW 7, non-shut-off
Microsart Base 47 mm
Materials stainless-steel, silicone O-ring
Suitable membrane filter diameter 47 mm
Filtration area (e. g. for the use 12.5 cm
with Microsart Funnels)
Ordering Information
Microsart Combi.jet 2-Branch Manifold, Made of High-Grade Stainless-Steel,
Without Frits and Funnels, to Accommodate Various Funnel Types
Description Order No.
Microsart Combi.jet 2-branch manifold, without base support 16848-CJ
and frits
Microsart Base 47 mm, with frit, stainless-steel base for 1ZU---0002
Combisart and Microsart Combi.jet
Optimized for the use with 47 mm membranes, Click-Fit closure for
Microsart Funnel, Microsart @filter and other funnel types sealed
by bayonet closure
Funnels and filter holders to connect onto the Microsart Combi.jet manifold are
equivalent to those for the use with the Combisart system (page 41).
Filtration Equipment Microbiological Enumeration 49
The filter stations are directly connected to the Transfer Pump for simultaneous transfer of the
filtrate to waste. Easy assembling thanks to Quick Connection technology.
Ordering Information
Pos. Description Qty. Order No. Detailed
Information
on Page
Microsart Combi.jet stainless-steel equipment: 48
1 Microsart Base 47 mm 2 1ZU---0002
2 Microsart Combi.jet 2-branch manifold 1 16848-CJ
Sterile venting of the filter station: 45
3 Minisart SRP25, 0.2 m 1 17575--------ACK
4 Silicone tubing, pressure-sided, 1 m 2* 1ZAS--0007 61
Silicone tubing, pressure-sided, 2 m 1ZAS--0019
Silicone tubing, pressure-sided, 10 m 1ZAS--0020
Vacuum Pump: 60
5 Microsart e.jet Transfer Pump, 1 166MP-4
230 V, 50 Hz
Additional accessories:
Microsart @filter 100, sterile filter 1 16D01--10-H6--TG 35
units, packaged on trays
Stainless-steel tweezers 1 16625 65
Colony Counter 1 17649 64
Incubator 1 18119 64
Container for anaerobic incubation 1 16671 65
Ordering Information
Pos. Description Qty. Order No. Detailed
Information
on Page
Combisart stainless-steel equipment: 41
1 Combisart single base, 50 mm 1 16840
2 Combisart 1-branch manifold 1 16844
Sterile venting of the filter station: 45
3 Minisart SRP25, 0.2 m 1 17575--------ACK
4 Rubber vacuum hose, 1 m 3* 16623 57
Suction flask and stopper: 56
5 Tube connector 1 17204
6 Silicone stopper 1 17173
7 Suction flask, 2 liters 1 16672
Water trap for pump protection: 57
8 Vacusart, 0.45 m 1 17804----------M
Vacuum Pump: 58
9 Microsart mini.vac, 230 V, 50 Hz 1 16694-2-50-06
Microsart mini.vac, 115 V, 60 Hz 16694-1-60-06
Additional accessories:
Microsart e.motion Dispenser 1 16712 13
Stainless-steel tweezers 1 16625 65
Colony Counter 1 17649 64
Incubator 1 18119 64
Stainless-steel prefilter attachment 1 16807 65
Container for anaerobic incubation 1 16671 65
* required length depends on distance between the filter station and the vacuum source
Filtration Equipment Microbiological Enumeration 51
Ordering Information
Pos. Description Qty. Order No. Detailed
Information
on Page
Combisart stainless-steel equipment: 41
1 Combisart single base, 50 mm 1 16840
2 Combisart 1-branch manifold 1 16844
Sterile venting of the filter station: 45
3 Minisart SRP25, 0.2 m 1 17575--------ACK
4 Silicone tubing with Quick Connection 1 1ZA---0006 61
Coupling, 20 cm, vacuum-sided
Silicone tubing with Quick Connection 1ZAS--0016
Coupling, 1 m, vacuum-sided
Vacuum Pump: 60
5 Microsart e.jet Transfer Pump 1 166MP-4
6 Silicone tubing, pressure-sided, 1 m 2* 1ZAS--0007 61
Silicone tubing, pressure-sided, 2 m 1ZAS--0019
Silicone tubing, pressure-sided, 10 m 1ZAS--0020
Additional accessories:
Microsart e.motion Dispenser 1 16712 13
Stainless-steel tweezers 1 16625 65
Colony Counter 1 17649 64
Incubator 1 18119 64
Stainless-steel prefilter attachment 1 16807 65
Container for anaerobic incubation 1 16671 65
Specifications
Stainless-steel quality High-grade stainless-steel: B.S. 304S31|AISI 304
Dimensions 3-branch manifold:
in mm (W|H|D) 3 + 100 ml: 432|184|120
3 + 500 ml: 442|262|132
6-branch manifold:
6 + 100 ml: 906|268|120
6+ 500 ml: 916|329|132
Max. operating pressure Vacuum or max. 2 bar pressure (29 psi)
Sterilization By autoclaving (max. 134C),
By dry heat (max. 180C),
By flaming,
By other methods acc. to ISO 8199
Parts and materials Lid, funnel, base part, filter support, clamp and tap made
of stainless-steel. Silicone flat gasket. Silicone lid seal
Flow rate per filter station 200 ml/min with 0.2 m membrane filter
for water at 90% vacuum 600 ml/min with 0.45 m membrane filter
Filtration area 12.5 cm2
Suitable membrane 50 mm
filter diameter (47 mm, if using a 47 mm frit filter support 6980103)
Outlet spouts 10 mm outside diameter
(individual system)
Outlet (branches only) Hose nipple, DN 10
54 Microbiological Enumeration Filtration Equipment
Ordering Information
Individual Stainless-Steel Filter Holders, Pre-Assembled with Stainless-Steel
Funnels and Lids
Description Capacity Order No.
Individual stainless-steel filter holder, 100 ml 1+ 100 ml 16219
Individual stainless-steel filter holder, 500 ml 1+ 500 ml 16201
Individual stainless-steel filter holder without lid, 40 ml 1+ 40 ml 16220
Ordering Information
Suction Flasks
Description Order No.
Suction flask, 5 liters acc. to DIN 12476, incl. stopper 75 D and glass tube 16672-----1
Suction flask, 2 liters acc. to DIN 12476, without stopper 16672
Tube connector for connecting a Combisart stainless-steel manifold 17204
to a suction flask 1 or 2 liters (not necessary when a Vacusart
is connected directly to the bored stopper)
Suction flask, 1 liter (not available in countries which have 16606
safety restrictions on glass hose nipples)
Replacement Parts
Description Order No.
Glass tube for silicon stopper 75 D for suction flask 5 liters 16672-----1 1EAQ--0017
Bored stopper 75 D for suction flask 5 liters 16672-----1 1EAS--0019
Assembling kit for hose barb for suction flask 5 liters 16672-----1 1EA---0018
Hose barb, complete, Polypropylene, for suction flask 2 liters 16672 6983003
Water Traps
Used between suction flask and vacuum
source, in order to prevent overflow of
filtrate into an electric vacuum pump.
Vacusart
Vacusart is a ready-to-connect filtration
unit, consisting of a polypropylene housing
and a hydrophobic, but air-permeable
PTFE membrane with a pore size of
0.45 m. Vacusart is perfectly suitable
for the protection of vacuum pumps.
It could be put directly into the hole of
the bored stopper and connected with
the rubber hose to the vacuum pump.
Ordering Information
Description Order No.
Vacusart water trap, pack of 3 17804----------M
Ordering Information
Description Order No.
Woulffs bottle, 500 ml 16610
Ordering Information
Description Order No.
Rubber vacuum hose (1 meter) 16623
58 Microbiological Enumeration Filtration Equipment
Specifications
Microsart maxi.vac Microsart mini.vac
16694-2-50-22 16694-2-50-06
16694-1-60-22 16694-1-60-06
Delivery 22 l/min 6 l/min
Ultimate Vacuum 100 mbar 100 mbar
Noise level [100 mbar] 57.559.0 dBA 53.5 dBA
Operating Pressure 1 bar 2.5 bar
Materials (contact with Aluminum, CR (Neoprene), PPS, EPDM, FPM
filtrate possible) NBR (Perbunan) (Fluoroelastomer)
Connectors for Tube (mm) ID 9 ID 4
Ambient Temperature 540C 540C
Mains 16694-2-50-22: 16694-2-50-06:
230 V|50 Hz 230 V|50 Hz
16694-1-60-22: 16694-1-60-06:
115 V|60 Hz 115 V|60 Hz
Motor Protection IP 44 IP 20
Power P1 130 W 65 W
Operating Current 0.9 A 0.63 A
Weight 7.1 kg 1.9 kg
Dimensions W|H|D (mm) 261|204|110 164|141|90
Recommended application All multi-branch manifolds Single filtration run up
to 3-branch manifolds
Filtration Equipment Microbiological Enumeration 59
Ordering Information
Description Order No.
Microsart maxi.vac for multiple filtration runs, 230 V, 50 Hz 16694-2-50-22
Microsart maxi.vac for multiple filtration runs, 115 V, 60 Hz 16694-1-60-22
Microsart mini.vac up to 3 filter stations in parallel, 230 V, 50 Hz 16694-2-50-06
Microsart mini.vac up to 3 filter stations in parallel, 115 V, 60 Hz 16694-1-60-06
Replacement Parts
Description Order No.
Replacement kit for 16694-2-50-22 and -1-60-22, 1ED---0055
set of one membrane, two valve springs and two head seals
Replacement kit for 16694-2-50-06 and -1-60-06, 1ED---0054
set of one membrane, two valve springs and two head seals
Sound absorber for 16694-2-50-22 and -1-60-22 1EH---0002
Sound absorber for 16694-2-50-06 and -1-60-06 1EH---0001
Fine adjustment head for 16694-2-50-22 and -1-60-22 1EV---0002
Fine adjustment head for 16694-2-50-06 and -1-60-06 1EV---0001
Fine adjustment head for 16694-2-50-06 and -1-60-06, 1EV---0003
for pressure filtration
60 Microbiological Enumeration Filtration Equipment
Specifications
Flow rate > 4.0 Nl/min
Max. vacuum 0.4 bar
Max. pressure 1.0 bar
Mains 100240 V|4763 Hz
Materials (in contact with filtrate) PTFE, ETFE, Polypropylene, EPDM, POM, PSU
Weight Pump: 1425.3 g
Power supply: 242.6 g
Dimensions (W|L|H) 120 + 170+190 mm
Max. ambient temp. +5+40C
Max. temp of liquid +5+80C
Max. viscosity <150 cSt
Protection type IP 64
Protection class III
Inlet|Outlet Quick Connection on hose nipples for DN 10 tubings
Filtration Equipment Microbiological Enumeration 61
1
5
2 3
4
Ordering Information
Description Order No. No. in
Picture
Microsart e.jet Transfer Pump with Quick Connection, 166MP-4 1
without tubings, inlet and outlet hose nipples for DN 10 tubings
Accessories
Description Order No. No. in
Picture
Tubing with Quick Connection Coupling (PSU), silicone, 1ZA---0006 2
20 cm, for vacuum-sided connection, inner diameter DN 10,
outer diameter DN 20, wall thickness 5 mm (when ordering,
please state length required in meters)
Silicone tubing with Quick Connection Coupling, 1 m, 1ZAS--0016
vacuum sided
Silicone tubing, 1 m, for pressure-sided connection, inner 1ZAS--0007 3
diameter DN 10, outer diameter DN 14, wall thickness 2 mm
Silicone tubing pressure-sided, 2 m 1ZAS--0019
Silicone tubing pressure-sided, 10 m 1ZAS--0020
Foot switch for Microsart e.jet Transfer Pump 1ZE---0053
Replacement Parts
Description Order No. No. in
Picture
Pump head complete for 166MP-3 and 166MP-4 1EP---0003
Power supply complete for 166MP-3 and 166MP-4 1EE---0012
Threaded Fittings
Quick Connection set, 2 Nipples (POM) on R3/8 male 1EAS--0027 4
thread and 2 Couplings (PSU) on DN 10 hose nipple
Quick Connection Nipple, stainless-steel 1EAS--0026 5
DN 10 hose nipple on R3/8 male thread 1EAF--0020
62 Microbiological Enumeration Filtration Equipment
Traditional Pumps
Description Order No.
Multiple filtration runs: 16612
13 mbar final vacuum, 26 l/min max., 220 V, 50 Hz
Multiple filtration runs: 16615
13 mbar final vacuum, 26 l/min max., 110 V, 60 Hz
Individual filtration run: 16692
100 mbar final vacuum, 20 l/min max., 220 V, 50 Hz
Individual filtration run: 16695
100 mbar final vacuum, 20 l/min max., 110 V, 60 Hz
Replacement Parts
Description Order No.
Set of two neoprene membranes, four valve springs 6986017
and two neoprene head seals for 16612/16615
Set of one neoprene membrane, two valve springs 6986105
and one neoprene head seal for 16692/16695
Rubber tubing 16623
Filtration Equipment Microbiological Enumeration 63
Ordering Information
Description Order No.
Water jet pump, with G 3/4 female thread 16611
Ordering Information
Description Order No.
Hand-operated vacuum pump with gauge 16673
Dosing Syringe
The most convenient way to moisten the is easily done by simply dropping the sinker
NPS with water is to use a dosing syringe at the end of the suction tubing into the
with an adapted Minisart syringe filter. water, then filling the dosing syringe and
Simultaneous sterilization and dispensing dispensing sterile water by operating the
of demineralized water in e.g. 3.5 ml steps trigger automatically.
Ordering Information
Description Order No.
Dosing syringe, 0.55 ml 16685-----2
Minisart, 0.2 m, individually, sterile-packaged 17597----------K
Replacement part: tubing with sinker for 16685-2 and 16685 6986125
Service Kit for Dosing Syringe 16685-----2 1EP---0002
64 Microbiological Enumeration Filtration Equipment
Colony Counter
Compact, handy battery-operated colony
counter, as simple to use as a ball point
pen, and has a 4-digit LCD-display.
The counter is supplied with an additional
marker refill.
Ordering Information
Description Order No.
Colony counter 17649
Replacement part: Black marker refill 6981540
Incubator
Compact, space-saving incubator for the The swing-up cover and removable
incubation of membrane filters on nutrient insertion plate simplify loading and
pads or other nutrient media. The incubator unloading. The cover is opaque, avoiding
has a capacity of 18 liters and is designed light penetration into the chamber.
to hold the following numbers and sizes of
petri dishes: 200+47 mm or 160+56 mm|
60 mm or 72+90 mm.
Specifications
Voltage 100240 V
Frequency 50|60 Hz
Rated power 0.045 kW
Weight 7.2 kg (15 lbs)
Max. shelf load 2 kg (4.4 lbs)
Dimensions W|H|D (mm) Inner 290|180|310
Outer 470|260|415
Temperature range 17C to 40C
Temperature stability at 37C Less than 0.2C
Temperature uniformity at 37C 1.2C
Capacity approx. 18 liters
Ordering Information
Description Order No.
Incubator 18119
Filtration Equipment Microbiological Enumeration 65
Stainless-Steel Tweezers
Membrane filters should only be handled can be flamed and are autoclavable.
with suitable tweezers to avoid contami They have blunt-edged tips for a careful,
nation since the stainless-steel tweezers firm hold of the membrane filter.
Ordering Information
Description Order No.
Stainless-steel tweezers 16625
Ordering Information
Description Order No.
Stainless-steel prefilter attachment 16807
Cellulose nitrate membranes with 50 mm diameter 11301--50----ACN
and 8 m pore size for the prefilter holder, pack of 100,
individually, sterile packaged
Replacement part: support plate, autoclavable, 6981139
flame treatment resistent
Ordering Information
Description Order No.
Anaerobic container 16671
Intro Sterility Testing 67
Intro
Sterility Testing
Table of Contents
78 EXPAND Training
Specifications
Pore size of the Sartochem 0.45 m, retention of 10 Million Serratia marcescens
membrane filter per cm2 of active filtration area
Filter area 5.7 cm2 in each Sterisart container
1
Flow rate (for water) 500 ml/min at 1 bar (approx. 15 psi)
Pore size of the air filters 0.2 m PTFE, validated acc. to HIMA
for the retention of B. diminuta
Sample container capacity 120 ml (graduation marks at 50, 75 and 100 ml)
Max. operating pressure 3 bar (approx. 44 psi) at 20C
Max. operating temperature 50C
Sterilization ETO (ethylene oxid gas) or gamma irradiation
Sterisart NF alpha
Disposable units for sterility testing in clean rooms, individually, sterile packaged,
ETO-sterilized, needles made of flame-sterilizable stainless-steel, 10 units
Ordering Information
Description Type of Type of Sample Order No.
Sample Container
Sterisart NF alpha with long LVPs Closed glass bottles 16466--------ACD
dual-needle metal spike, with septum
sterile-vented
Sterisart NF alpha with long LVPs Open containers (i.e. 16467--------ACD
needle and protective plate, SVPs glass ampoules, glass
inclusive sterile venting needle bottles), collapsible bags
Sterisart NF alpha with Luer or Medical Tubing systems and 16468--------ACD
Luer Lock connector, inclusive devices bags with Luer or
long needle and sterile venting Luer Lock connectors
needle
Sterisart NF Sterility Test Consumables Sterility Testing 71
Sterisart NF gamma
Disposable units for sterility testing in isolators, individually sterile, double-packaged,
gamma irradiated, needles made of flame-sterilizable stainless-steel, 10 units.
Ordering Information
Description Type of Type of Sample Order No.
Sample Container
Sterisart NF gamma with long LVPs Closed glass 16466--------GBD
dual-needle metal spike, bottles with
sterile-vented septum
Sterisart NF gamma with long LVPs Open containers 16467--------GBD
needle and protective plate, SVPs (i.e. glass ampoules,
inclusive sterile venting needle glass bottles),
collapsible bags
Sterisart NF gamma with Medical Tubing systems 16468--------GBD
Luer or Luer Lock connection, devices and bags with
inclusive, long needle and Luer or Luer Lock
sterile venting needle connectors
Sterisart NF gamma with Pre-filled Syringes with and 16469--------GBD
syringe-adapter and long syringes without needles
dual-needle metal spike,
sterile-vented
Sterisart NF gamma with Lyophilisates, Closed glass vials 16475--------GBD
two dual-needle metal spikes soluble with septum
of different length, one is powders,
sterile-vented liquid
antibiotics
Sterisart NF gamma with short SVPs Closed glass vials 16476--------GBD
dual-needle metal spike, with septum
sterile-vented
Sterisart NF gamma with long LVPs, Closed plastic 16477--------GBD
needle (side opening, with SVPs, containers|vials|
solid pointed tip, non-coring), eye drops ampoules, plastic
protective plate, inclusive sterile containers of Blow-
venting needle Fill-Seal fillings
Sterisart NF gamma with NEW Containers|bags 16478--------GBD
female Luer Lock connector Medical with Luer Lock
devices male connectors
72 Sterility Testing Sterisart NF Sterility Test Consumables
Disposable units for sterility testing in isolators, Sterisart NF containers with integrated
septum for reliable sterile sampling during incubation. Individually sterilized, double-
packaged, gamma irradiated, needles made of flame-sterilizable stainless-steel, 10 units.
Ordering Information
Description Type of Type of Sample Order No.
Sample Container
Sterisart NF gamma Septum LVPs Closed glass bottles 16466--------GSD
with long dual-needle metal with septum
spike, sterile-vented
Sterisart NF gamma Septum LVPs Open containers 16467--------GSD
with long needle and protective SVPs (i.e. glass ampoules,
plate, inclusive sterile venting glass bottles),
needle collapsible bags
Sterisart NF gamma Septum Pre-filled Syringes with and 16469--------GSD
with syringe-adapter and syringes without needles
long dual-needle metal spike,
sterile-vented
Sterisart NF gamma Septum Lyophilisates, Closed glass vials 16475--------GSD
with two dual-needle spikes Soluble with septum
of different length, one is powders,
sterile-vented Liquid
antibiotics
Sterisart NF gamma Septum SVPs Closed glass vials 16476--------GSD
with short dual-needle, with septum
sterile vented
Sterisart NF Sterility Test Consumables Sterility Testing 73
Accessories
Description Application Order No.
Sterisart NF gamma tubing Dissolving and diluting of 16470--------GBD
system with two dual-needle hardly soluble powders in
metal spikes of different length, closed containers
needles made of flamable
stainless-steel
Needle with venting filter, Sterile venting of containers with 16596--------HNK
4 cm, stainless-steel, rinsing solution and nutrient
individually sterile packaged, media, additional sterile venting
gamma-irradiated, needles, equal to the included
pack size 50 needles of the Sterisart NF units,
i.e. type 16467, 16468 and 16477
Specifications
Pump flow rate 70650 ml/min
Power requirements 100240 VAC
Frequency 5060 Hz
Power consumption 100 W
Dimensions
Pump approx. 336+260 + 210 mm (with lever) (W+ D+ H)
Pump with holding ring for
bottles, container approx. 440+365+ 485 mm (W+D + H)
Weight
Basic version 16419 approx. 13.5 kg
Upgraded version 16420 with
display and user software approx. 14.6 kg
Ordering Information
Description Order No.
Sterisart Universal pump, basic version 16419
Sterisart Universal pump, upgraded version 16420
with display and user software
Accessories
Description Order No.
Footswitch 1ZE---0033
Adapter for Sterisart NF units, fitting into container 1ZG---0014
for draining of Millipore Equinox pump
Transport trolley 1ZE---0039
Communication kit 1ZE---0040
Installation kit for isolators 1ZE---0050
Container for draining 1ZG---0028
Drain Tubing 1ZA---0002
Multi-packaged, ETO-sterile
The autoclaved filled bottles are packaged
into a polypropylene box which is then
sealed into a Stericlin bag and sterilized by
ethylene oxide (ETO). This treatment pro-
vides a sterile outer surface of the bottles
and the packaging. The multiple-packaging
allows the transfer of the bottles into clean
rooms from a non-sterile area into a
sterile area.
EXPAND
Training Courses and Seminars
Practical Exercises
Sterility testing of different sterile
products (LVPs|SVPs|ampoules|
syringes|medical devices)
Performing sterility test in isolators
EXTEND Instrument Services Sterility Testing 79
The reliability and the service life of your Prerequisites Required by the Customer
equipment significantly depend on its System is standing at the installation
installation and configuration according location
to the specifications. Unobstructed access to the system
Power is available at the installation
Installation and commissioning by location
our service professionals will ensure When ordering, a responsible person has
that your Sterisart pump successfully to be appointed
operates right from the start.
Specifications
Check of scope of delivery
Check of device for transport damage
Manufacturers ID label check
Installation of system components and
utilities connection
P ositioning of the system at the installa-
tion location (not installation in isolator*)
Initial start-up
B rief instructions to operating personnel
(not application training)
General maintenance discussion
Ordering Information
Description Order No.
Installation and Commissioning Sterisart Pump S8740INST
80 Sterility Testing EXTEND Instrument Services
Preventive Maintenance
Sterisart Pump
Specifications
Inspection visit at fixed intervals
or as needed
Visual inspection
Maintenance check according to
maintenance checklist
Check of flow rate capacity and
uniform performance
Start-up test
CONFIDENCE Validation Services Sterility Testing 81
Mycoplasma
Contamination Control
Table of Contents
87 Microsart AMP Mycoplasma
Media Preparation
Sampling
Fermentation Harvest
Specifications
Each kit contains reagents for 25 or 100 reactions. The expiration date of the unopened
package is specified on the package label. The kit components are to be stored until
use at +2 to +8C and must be stored after opening and rehydration below 18C.
The lot-specific Certificate of Analysis can be downloaded from the manufacturers
website (www.minerva-biolabs.com).
Ordering Information
Mycoplasma Kits
Description Quantity Order No.
Microsart AMP Mycoplasma 25 tests SMB95-1001
Microsart AMP Mycoplasma 100 tests SMB95-1002
Accessories
Microsart AMP Extraction 50 extractions SMB95-2003
Vivaspin 6, 100,000 MWCO 25 units VS0641
Vivaspin 20, 100,000 MWCO 12 units VS2041
Microsart AMP Coating Buffer 20 + 2 ml SMB95-2002
Microsart Validation Standard 3 vials SMB95-2011
Mycoplasma arginini
Microsart Validation Standard 3 vials SMB95-2012
Mycoplasma orale
Microsart Validation Standard 3 vials SMB95-2013
Mycoplasma gallisepticum
Microsart Validation Standard 3 vials SMB95-2014
Mycoplasma pneumoniae
Microsart Validation Standard 3 vials SMB95-2015
Mycoplasma synoviae
Microsart Validation Standard 3 vials SMB95-2016
Mycoplasma fermentans
Microsart Validation Standard 3 vials SMB95-2017
Mycoplasma hyorhinis
Microsart Validation Standard 3 vials SMB95-2018
Acholeplasma laidlawii
Microsart Validation Standard 3 vials SMB95-2019
Spiroplasma citri
Microsart Calibration Reagent 1 vial SMB95-2021
Mycoplasma arginini
Microsart Calibration Reagent 1 vial SMB95-2022
Mycoplasma orale
Microsart Calibration Reagent 1 vial SMB95-2023
Mycoplasma gallisepticum
Microsart Calibration Reagent 1 vial SMB95-2024
Mycoplasma pneumoniae
Microsart Calibration Reagent 1 vial SMB95-2025
Mycoplasma synoviae
Microsart Calibration Reagent 1 vial SMB95-2026
Mycoplasma fermentans
Microsart Calibration Reagent 1 vial SMB95-2027
Mycoplasma hyorhinis
Microsart Calibration Reagent 1 vial SMB95-2028
Acholeplasma laidlawii
Microsart Calibration Reagent 1 vial SMB95-2029
Spiroplasma citri
90 Mycoplasma Contamination Control Microsart ATMP Mycoplasma
Applications
The Microsart ATMP Mycoplasma real-
time PCR kit is especially designed for
all hospitals, institution and companies
which are involved in testing Mycoplasma
contaminatioin in ATMPs according to
EP2.6.7. or many other international guide-
lines like USP <63> and JP.
Specifications
Each Kit contains all required reagents for 25 or 100 reactions including polymerase as
part of the Mycoplasma Mix. The expiration date of the unopened package is specified on
the package label. The kit components are to be stored until use at +2 to +8C and must
be stored after opening and rehydration below -18C. The lot-specific Certificate of
Analysis can be downloaded from the manufacturers website (www.minerva-biolabs.com).
Ordering Information
Mycoplasma Kits
Description Quantity Order No.
Microsart ATMP Mycoplasma 25 SMB95-1003
Microsart ATMP Mycoplasma 100 SMB95-1004
Accessories
Microsart AMP Extraction 50 extractions SMB95-2003
Vivaspin 6, 100,000 MWCO 25 units VS0641
Vivaspin 20, 100,000 MWCO 12 units VS2041
Microsart AMP Coating Buffer 20 + 2 ml SMB95-2002
Microsart Validation Standard 3 vials SMB95-2011
Mycoplasma arginini
Microsart Validation Standard 3 vials SMB95-2012
Mycoplasma orale
Microsart Validation Standard 3 vials SMB95-2013
Mycoplasma gallisepticum
Microsart Validation Standard 3 vials SMB95-2014
Mycoplasma pneumoniae
Microsart Validation Standard 3 vials SMB95-2015
Mycoplasma synoviae
Microsart Validation Standard 3 vials SMB95-2016
Mycoplasma fermentans
Microsart Validation Standard 3 vials SMB95-2017
Mycoplasma hyorhinis
Microsart Validation Standard 3 vials SMB95-2018
Acholeplasma laidlawii
Microsart Validation Standard 3 vials SMB95-2019
Spiroplasma citri
Microsart Calibration Reagent 1 vial SMB95-2021
Mycoplasma arginini
Microsart Calibration Reagent 1 vial SMB95-2022
Mycoplasma orale
Microsart Calibration Reagent 1 vial SMB95-2023
Mycoplasma gallisepticum
Microsart Calibration Reagent 1 vial SMB95-2024
Mycoplasma pneumoniae
Microsart Calibration Reagent 1 vial SMB95-2025
Mycoplasma synoviae
Microsart Calibration Reagent 1 vial SMB95-2026
Mycoplasma fermentans
Microsart Calibration Reagent 1 vial SMB95-2027
Mycoplasma hyorhinis
Microsart Calibration Reagent 1 vial SMB95-2028
Acholeplasma laidlawii
Microsart Calibration Reagent 1 vial SMB95-2029
Spiroplasma citri
92 Mycoplasma Contamination Control Microsart RESEARCH Mycoplasma
Specifications
Each kit contains reagents for 25 or 100 reactions. The expiration date of the unopened
package is specified on the package label. The kit components are to be stored until
use at +2 to +8C, and must be stored after opening and rehydration below 18C.
The lot-specific Certificate of Analysis can be downloaded from the manufacturers
website (www.minerva-biolabs.com).
Ordering Information
Mycoplasma Kits
Description Quantity Order No.
Microsart RESEARCH Mycoplasma 25 SMB95-1005
Microsart RESEARCH Mycoplasma 100 SMB95-1006
Accessories
Microsart AMP Extraktion 50 extractions SMB95-2003
Microsart Calibration Reagent 1 vial SMB95-2021
Mycoplasma arginini
Microsart Calibration Reagent 1 vial SMB95-2022
Mycoplasma orale
Microsart Calibration Reagent 1 vial SMB95-2023
Mycoplasma gallisepticum
Microsart Calibration Reagent 1 vial SMB95-2024
Mycoplasma pneumoniae
Microsart Calibration Reagent 1 vial SMB95-2025
Mycoplasma synoviae
Microsart Calibration Reagent 1 vial SMB95-2026
Mycoplasma fermentans
Microsart Calibration Reagent 1 vial SMB95-2027
Mycoplasma hyorhinis
Microsart Calibration Reagent 1 vial SMB95-2028
Acholeplasma laidlawii
Microsart Calibration Reagent 1 vial SMB95-2029
Spiroplasma citri
94 Mycoplasma Contamination Control Microsart AMP Extraction
Specifications
Kit Component Quantity Required Supplements
Spin columns 50
Collection Tubes 350
Sample Storage Tubes 50
Buffer A1 15 ml
Buffer A2 25 ml
Buffer B 15 ml 15 ml Ethanol
Buffer C 12 ml 48 ml Ethanol
Buffer D 22 ml
Ordering Information
Description Quantity Order No.
Microsart AMP Extraction 50 SMB95-2003
Microsart Coating Buffer Mycoplasma Contamination Control 95
Ordering Information
Description Quantity Order No.
Microsart Coating Buffer 202 ml SMB95-2002
96 Mycoplasma Contamination Control Microsart Validation Standard
Specifications
Kit Component Quantity Cap Colour
1 Mycoplasma species or 3 tubes with green
1 Acholeplama species or 10 CFU each,
1 Spiroplasma species lyophilized
Negative Control 2 tubes with white
lyophilization matrix
as negative control,
lyophilized
Ordering Information
Description Order No.
Microsart Validation Standard SMB95-2011
Mycoplasma arginini
Microsart Validation Standard SMB95-2012
Mycoplasma orale
Microsart Validation Standard SMB95-2013
Mycoplasma gallisepticum
Microsart Validation Standard SMB95-2014
Mycoplasma pneumoniae
Microsart Validation Standard SMB95-2015
Mycoplasma synoviae
Microsart Validation Standard SMB95-2016
Mycoplasma fermentans
Microsart Validation Standard SMB95-2017
Mycoplasma hyorhinis
Microsart Validation Standard SMB95-2018
Acholeplasma laidlawii
Microsart Validation Standard SMB95-2019
Spiroplasma citri
Microsart Calibration Reagent Mycoplasma Contamination Control 97
For quantitative PCR titrated genomic DNA extracted with a classic phenol-chloroform
can serve for creating standard curves by protocol and further purified with adsorp-
using dilution series of the Microsart tion chromatography. The concentration of
Calibrations Reagents as sample in the PCR. the DNA was quantified photometrically
The cycler specific software can then be (OD260/280) and with an ultrasensitive flu-
used to determine unknown DNA concen- orescent nucleic acid stain for quantitating
trations. double-stranded DNA (dsDNA) calibrated
to weight reference standards and con-
This product provides isolated genomic trolled by qPCR (compared against exactly
DNA of a specific Mycoplasma species. quantified calibrator plasmids). The DNA
To obtain these highly purified genomic concentration was adjusted with regular
DNA standards, a special liquid culture TE80 buffer prior to the lyophilization.
medium was inoculated with early passage
strains and harvested at the end of the
logarithmic growth phase by repeated
washing and centrifugation. The DNA was
Specifications
Kit Component Quantity Cap Colour
1 Mycoplasma species or 1 tube with green
1 Acholeplasma species or 108 genome copies,
1 Spiroplasma species lyophilized
Buffer 32 ml tubes white
Ordering Information
Description Order nr.
Microsart Calibration Reagent SMB95-2021
Mycoplasma arginini
Microsart Calibration Reagent SMB95-2022
Mycoplasma orale
Microsart Calibration Reagent SMB95-2023
Mycoplasma gallisepticum
Microsart Calibration Reagent SMB95-2024
Mycoplasma pneumoniae
Microsart Calibration Reagent SMB95-2025
Mycoplasma synoviae
Microsart Calibration Reagent SMB95-2026
Mycoplasma fermentans
Microsart Calibration Reagent SMB95-2027
Mycoplasma hyorhinis
Microsart Calibration Reagent SMB95-2028
Acholeplasma laidlawii
Microsart Calibration Reagent SMB95-2029
Spiroplasma citri
Intro Air Monitoring 99
Air Monitoring
Table of Contents
102 BACTair
106 Accessories
Find out more about our Air Sampling solution and our
extensive service package:
U nique Gelatine Membrane Filter (GMF) for sampling
of microorganisms, viruses and bacteriophages
Portable, battery powered AirPort MD8
B ACTAir Media plates for direct sampling and
incubation
EXTEND Instrument Services
Gelatine Membrane Filters (GMF) Air Monitoring 101
Gelatine filters in conjunction with the Gelatine filters in conjunction with the
MD8 air samplers (gelatine filter method) MD8 air samplers offer the following
are used for the collection of airborne features and benefits:
microbes and viruses. Gelatine filter Absolute retention rate (99.9995%
disposables are individually packed, for Bac. sub. niger spores, 99.94% for
pre-sterilized and ready-to-connect units, T3 phages)
each consisting of a gelatine membrane The filter maintains the viability of
filter and a holder. collected microorganisms for a relevant
and meaningful sampling time
Gelatine membrane filters are still available Gelatine filters are completely water-
as filter discs, suitable for the filter holder soluble. Therefore, microbes in one sam-
17655 (80 mm diameter) supplied with the ple can be cultivated in|on different
MD8 airscan air samplers, as well as in nutrient media or low and high bacteria
smaller diameters. For DNA-based appli- counts can be measured. The sample is
cations such as detecting viruses, dissolvable not affected by inhibitors
DNA-free gelatine membranes are available The solubility of the gelatine filter is a
on request ([email protected]). prerequisite for virus sampling
Specifications
Gelatine filters ater soluble, pore size 3 m, 80 mm diameter,
W
thickness approx. 250 m
Thermal resistance Max. 60C
Air flow rate Approx. 2.7 l/min./cm2 at P = 0.05 bar
Retention rates 1. Bac. subtilis niger spores 99.9995% at 0.25 m/s inlet velocity.
2. Coli phages: phage T1, 99.9% at 0.3 m/s inlet velocity
and 50% rel. air humidity. Phage T3, 99.94% at 0.3 m/s inlet
velocity and 80% rel. humidity.
Filtration area 38.5 cm2
Conditions for use Room temperature, max. 30C, max. air humidity 85%
Sterilization Supplied pre-sterilized by gamma irradiation
Ordering Information
Disposable Gelatine Units, Sterile, Pack of 10
Description Order No.
Individually packed in 1 polyethylene bag each 17528--80----ACD
Individually packed in 3 polyethylene bags each 17528--80----BZD
Individually packed in 3 polyethylene bags each, 17528--80----VPD
but label on innermost bag
Gelatine Disc Filter, Sterile, Sealed in Units of Five Each in a Polyethylene Bag
Diameter Package Size Order No.
80 mm 50 12602--80----ALK
50 mm 100 12602--50----ALN
50 mm 50 12602--50----ALK
47 mm 100 12602--47----ALN
47 mm 50 12602--47----ALK
37 mm 50 12602--37----ALK
A new developed system for sampling This new method uses the AirPort MD8
airborne organisms that allows impaction air sampler to draw the air stream over
onto culture media plates, where the the BACTair Culture Media Plates.
plates function directly as collection BACTair is ready-to-connect to the
heads. This means that the collection AirPort MD8.
properties are integrated right into the
culture media plates. Metal sieve plates BACTair offers the following benefits:
or metal collection heads with slots, Individually, sterile packaged
which have to be sterilized for routine Integrated disposable sieve
samplings on a regular basis, are elimi- Pre-filled with agar media
nated. Now, non-sterile sieves or slots Samples 1 m3 in just 8 min
have become a thing of the past. Optimized geometry
Specifications
Material Polystyrene
Dimensions 116+ 24 mm
Number of impaction holes 400 holes, d 0.47 mm each
High retention of particles > 0.65 m
Sterilization Gamma irradiation
Ordering Information
BACTair Culture Media Plates with Agar, 110 mm, Individually,
Sterile Packaged, 10 Units
Determination of Medium Type Order No.
Total Count Tryptic Soy Agar (TSA) 14320-110----ACD
Yeasts and molds Sabouraud Agar (acc. USP) 14321-110----ACD
Air Sampler
Description Order No.
AirPort MD8 Air Sampler for BACTair incl. charger 16757
Special brochures are available on request. Order no. SM-4023-e and SL-2047-e
Air Sampler Air Monitoring 103
MD8 airscan
Air Sampler for Critical Applications
The system consists of the MD8 airscan The MD8 airscan air sampler allows
air sampler and disposable gelatine filter the user to make selective adjustments and
units. The system is routinely used for the easily sample air flow rate and removal
quantitative detection of air-borne organ- speed? By means of a specially developed
isms, mainly at filling lines in sterile areas calibration unit (see accessories), the user
of class A (classification according to EU can calibrate the MD8 airscan locally,
Guide for GMP), isolators, or blow-fill-seal e.g. within the scope of validation steps.
machines.
After removing the sample, the gelatine
The exceptionally high air flow rate of filter can be placed directly on the agar
8 m3/h enables isokinetic sampling at flow culture medium for incubation and colony
rates that are usual in laminar flow as growth.
well as filtration of 1 m3 air very quickly
(less than 8 minutes). The filter unit can
be placed separately from the air sampler
for remote sampling.
Specifications
MD8 airscan Air Sampler
Air flow rate 2.0 m3/h 8 m3/h adjustable in 100-liter steps
Timer 199 minutes, adjustable in 1-minute steps
Max. deviation 5% in a temperature range of 1535C
Noise level For gelatine membrane filters, max. 62 dB (A)
Weight Approx. 6.5 kg
Dimensions (L+ W+H) 375+ 242+ 228 mm
Correction of the air flow When the entered air flow rate cannot be attained,
rate setting the display shows the max. attainable flow
rate for a corresponding new setting below this value.
Included filter holder 17655 (Gelatine disc filters)
The MD8 airscan (16746; 16747) is not in compliance with the Restriction of Hazardous
Substances Directive (RoHS, 2011/65/EU) and therefore its (spare part) availability is
restricted for exchange and maintenance only.
104 Air Monitoring Air Sampler
Ordering Information
MD8 airscan Air Sampler
Description Order No.
MD8 airscan air sampler, 230 V, 50 Hz 16746
MD8 airscan air sampler, 115 V, 60 Hz 16747
Consumables
Disposable gelatine units, sterile, pack of 10 Order No.
Individually packed in 1 polyethylene bag each 17528--80----ACD
Individually packed in 3 polyethylene bags each 17528--80----BZD
Individually packed in 3 polyethylene bags each, 17528--80----VPD
but label on innermost bag
The MD8 airscan (16746; 16747) is not in compliance with the Restriction of Hazardous
Substances Directive (RoHS, 2011/65/EU) and therefore its (spare part) availability is
restricted for exchange and maintenance only.
Air Sampler Air Monitoring 105
AirPort MD8
Specifications
AirPort MD8
Volume flow regulation By an integrated impeller wheel.
Volume flow adjustable 30 l/min., 40 l/min., 50 l/min. and 125 l/min.
Fixed given sample volumes 25, 50, 100, 250, 500, 750 and 1000 liters. In addition,
the sample volume can be chosen manually in 5-liter
steps.
Operational life with Approx. 4.5 hours for 50 l/min
one battery battery charge
Noise level For gelatine membrane filters 48 dB (A)
Weight Approx. 2.5 kg
Dimensions (L +W + H) 300+ 135+ 165 mm
Inclusive adapter 17801 (for disposable gelatine filter units)
Power Supply
Battery NiMH 16.8 Volt/3800 mAh
Battery charger input 100240 V/4763 Hz/600 mA
Battery charger output 24 V/1000 mA
Charging time Approx. 4.5 hours for empty battery
Ordering Information
AirPort MD8
Description Order No.
AirPort MD8, complete with two adapters (17801 and 17803) 16757
and battery charger (69898525).
Accessories
For the MD8 Air Samplers
Calibration Unit
The user can calibrate the MD8 airscan supplied complete with battery charger |
and AirPort MD8 directly on the job by power supply unit (specific for the country
means of the calibration unit*. in which it is used), filter holder, connec-
tors set and connection tube (PVC, 2 m).
This is absolutely necessary above all within
the scope of validation steps, for which it * Alternatively, a maintenance agreement can
be signed. Within the scope of the contractual
is important that the shown air flow rate
services, Sartorius technicians will carry out a
(desired value at the MD8) corresponds to calibration of the MD8 at regular intervals.
the actual air amount (actual value at the
calibration device). The calibration unit is
Specifications
Calibration Unit
Dimensions L ength, 300 mm (without filter holder),
Width, 390 mm with handles
Height, 182 mm min., 200 mm max. (adjustable feet)
Connectors Quick locks (bayonet principle)
Operational life with full battery Approx. 4 hours
Charge time for empty battery Approx. 10 hours
Measuring range 116 m3/h
Max. error 116 m3/h, 2%
Type of protection IP 40
Allowable ambient temperature Min. 0C, max. 40C
Weight Approx. 11 kg
Ordering Information
Description Order No.
Calibration unit for the MD8 air samplers 16756
The MD8 airscan (16746; 16747) is not in compliance with the Restriction of Hazardous
Substances Directive (RoHS, 2011/65/EU), and therefore its (spare part) availability is
restricted for exchange and maintenance only.
Accessories Air Monitoring 107
Ordering Information
Description Order No.
Flexible PVC hose with reinforced ends (2 m) 17085
Flexible PVC hose with reinforced ends (5 m) 17088
Silicone tubing, sterilizable (1 m, state length required) 17662
Set of connectors (consisting of 17658 and 17659), aluminum 17657
Connector (air sampler inlet to flexible hose), aluminum 17658
Connector (flexible hose to filter holder|adapter), aluminum 17659
Case
A stable case for the transport and storage
of a MD8 airscan, incl. accessories.
Ordering Information
Description Order No.
Case for MD8 airscan 17208
Aluminum Stack
It consists of a middle part, 10 numbered middle part. After removing the sample,
filter holders and 2 end caps. The stack is the inserted filter holders are put on the
first sterilized (by 180C dry heat, 2 h), and other side of the middle part, so that used
then equipped with the filters under sterile and unused filter holders are separated
conditions (LF cleanbench). The prepared from each other.
filter holders are put on one side of the
Ordering Information
Aluminium Stack
Description Order No.
Aluminum stack for MD8 air samplers 17656
Replacement Parts
Description Order No.
Individual filter holders for gelatine filter type 12602--80----ALK 17655
Middle part 17660
End cap 17661
The MD8 airscan (16746; 16747) is not in compliance with the Restriction of Hazardous
Substances Directive (RoHS, 2011/65/EU) and therefore its (spare part) availability is
restricted for exchange and maintenance only.
108 Air Monitoring Accessories
Ordering Information
Description Order No.
Adapter (DN 25 hose barb to 11 1/2 sanitary flange) to connect 17016
MD8 airscan to an isolator via silicone tubing and a filter capsule,
stainless-steel
Adapter (DN 30 hose barb to 11 1/2 sanitary flange) to connect 17030
MD8 airscan to an isolator via flexible PVC hose and filter capsule,
stainless-steel
Clamp for 11 1/2 sanitary flanges, stainless-steel Clamp 17033
for 11 1/2 sanitary flanges, stainless-steel
Connector (flexible hose to filter holder|adapter), hose nipple, 17659---001
stainless-steel
Connector (flexible hose to filter holder|adapter), tri clamp, 17659---003
stainless-steel
Adapter for gelatine filter disposables, stainless-steel 17801---001
Sartofluor MidiCap Capsule with PTFE membrane and sanitary 5185307TS-----SS
flange inlet and outlet, for sterile air filtration inserted between
the MD8 airscan and isolator
Ordering Information
Description Order No.
Remote control (Interface) for MD8 airscan designed for PLC units 1ZE---0003
Remote control for MD8 airscan for use with PC 1ZE---0004
(dialog system software)
The MD8 airscan (16746; 16747) is not in compliance with the Restriction of Hazardous
Substances Directive (RoHS, 2011/65/EU) and therefore its (spare part) availability is
restricted for exchange and maintenance only.
Accessories Air Monitoring 109
Ordering Information
Gelatine Disc Filters, 3 m Pore Size, 80 mm, 50 Pieces/Pack
Description Order No.
Gelatine disc filter, sterile, sealed in units of five each in 12602-080 ALK
a polyethylene bag
Ordering Information
Cellulose Nitrate Membrane Filters, 80 mm Diameter, 100 Pieces/Pack
Description Order No.
Cellulose nitrate membrane filters, 0.8 m, white with black grid, 11404--80----ALN
pre-sterilized in bags of 5
Cellulose nitrate membrane filters, 0.8 m, grey with white grid, 13004--80----ALN
pre-sterilized in bags of 5
Cellulose nitrate membrane filters, 8 m, white no grid, 11301--80----ALN
pre-sterilized in bags of 5
The MD8 airscan (16746; 16747) is not in compliance with the Restriction of Hazardous
Substances Directive (RoHS, 2011/65/EU) and therefore its (spare part) availability is
restricted for exchange and maintenance only.
110 Air Monitoring EXTEND Instrument Services
Preventive Maintenance
AirPort MD8|MD8 airscan
The reliability and the service life of your Prerequisites Required by the Customer
equipment significantly depend on its System is standing at the installation
installation and configuration according location
to the specifications. Unobstructed access to the system
Power is available at the installation
Installation and commissioning by our location
service professionals will ensure that When ordering, a responsible person has
your MD8 successfully operates right to be appointed
from the start.
Specifications
Check of scope of delivery
Check of device for transport damage
Manufacturers ID label check
Initial start-up
B rief instructions to operating personnel
(not application training)
General maintenance discussion
Ordering Information
Description Order No.
Installation and Commissioning AirPort MD8|MD8 airscan S8739INST
The MD8 airscan (16746; 16747) is not in compliance with the Restriction of Hazardous
Substances Directive (RoHS, 2011/65/EU) and therefore its (spare part) availability is
restricted for exchange and maintenance only.
Intro Chemical Compatibility 113
Chemical Compatibility
Table of Contents
1. Filter Materials
Acids
Acetic acid, 25% ?
Acetic acid, 96% ? ?
Hydrofluoric acid, 25% ? ?
Hydrofluoric acid, 50% ? ?
Perchloric acid, 25% ? ? ?
Phosphoric acid, 25% ? ? ?
Phosphoric acid, 85% ? ?
Nitric acid, 25% ?
Nitric acid, 65% ?
Hydrochloric acid, 25% ?
Hydrochloric acid, 37% ?
Sulfuric acid, 25% ?
Sulfuric acid, 98% ? ?
Trichloroacetic acid, 25% ? ? ?
Bases
Ammonium, 1N
Ammonium hydroxide, 25%
Potassium hydroxide, 32%
Sodium hydroxide, 32%
Sodium, 1N
Aqueous Solutions
Formaline, 30%
Sodium hypochlorite, 5% ? ?
Hydrogen peroxide, 35% ? ? ?
Key to Symbols
= compatible = limited compatibility
= not compatible ? = not tested
E = compatible after replacing silicone O-ring with an EPDM O-ring
V = compatible after replacing the silicone O-ring with a Fluoroelastomer O-ring
Acids
Acetic acid, 25%
Acetic acid, 96% ?
Hydrofluoric acid, 25%
Hydrofluoric acid, 50%
Perchloric acid, 25%
Phosphoric acid, 25%
Phosphoric acid, 85%
Nitric acid, 25%
Nitric acid, 65%
Hydrochloric acid, 25%
Hydrochloric acid, 37%
Sulfuric acid, 25%
Sulfuric acid, 98%
Trichloroacetic acid, 25%
Bases
Ammonium, 1N
Ammonium hydroxide, 25%
Potassium hydroxide, 32%
Sodium hydroxide, 32%
Sodium, 1N
Aqueous Solutions
Formaline, 30%
Sodium hypochlorite, 5%
Hydrogen peroxide, 35%
Key to Symbols
= compatible = limited compatibility
= not compatible ? = not tested
Index
Products for
Microbiological Control Laboratory Weighing Products Sartorius Liquid
Laboratory Handling
Weighing Products
Products
Laboratory Water
Laboratory Filtration Products Purification Products
Europe Americas
Germany Italy USA Hong Kong
Sartorius Lab Instruments Sartorius Italy S.r.l. Sartorius Corporation Sartorius Hong Kong Ltd.
GmbH & Co. KG Viale A. Casati, 4 5 Orville Drive, Suite 200 Unit 1012, Lu Plaza
Otto-Brenner-Strasse 20 20835 Muggi (MB) Bohemia, NY 11716 2 Wing Yip Street
37079 Goettingen Kwun Tong
Phone +39.039.4659.1 Phone +1.631.254.4249 Kowloon, Hong Kong
Phone +49.551.308.0 Fax +39.039.4659.88 Toll-free +1.800.635.2906
Fax +49.551.308.3289 Fax +1.631.254.4253 Phone +852.2774.2678
Fax +852.2766.3526
Netherlands
France & Suisse Romande Sartorius Netherlands B.V. Argentina
Sartorius France Sartorius Argentina S.A. India
Phone +31.30.60.53.001
2, rue Antoine Laurent de Lavoisier Int. A. valos 4251 Sartorius Weighing India Pvt. Ltd.
Fax +31.30.60.52.917
ZA de la Gaudre B1605ECS Munro #69/2-69/3, NH 48, Jakkasandra,
91410 Dourdan [email protected] Buenos Aires Nelamangala Tq
562 123 Bangalore, India
Phone +33.1.70.62.50.00 Phone +54.11.4721.0505
Fax +33.1.64.59.76.39 Fax +54.11.4762.2333 Phone +91.80.4350.5250
Poland
Fax +91.80.4350.5253
Sartorius Poland sp.z o.o.
ul. Wrzesinska 70
Austria 62-025 Kostrzyn Brazil
Sartorius Austria GmbH Sartorius do Brasil Ltda Japan
Modecenterstrasse 22 Phone +48.61.6473830 Avenida Senador Vergueiro 2962 Sartorius Japan K.K.
1030 Vienna Fax +48.61.6473839 So Bernardo do Campo 4th Fl., Daiwa Shinagawa North Bldg.
CEP 09600-000 - SP- Brasil 8-11, Kita-Shinagawa 1-chome
Phone +43.1.7965760.0 Shinagawa-ku, Tokyo, 140-0001 Japan
Fax +43.1.7965760.24 Russian Federation Phone +55.11.4362.8900
Fax +55.11.4362.8901 Phone +81.3.3740.5408
LLC Sartorius RUS
Fax +81.3.3740.5406
Uralskaya str. 4, Lit. B
Belgium 199155 St. Petersburg
Sartorius Belgium N.V. Canada
Rue Colonel Bourg 105 Phone +7.812.327.53.27 Sartorius Canada Inc. Malaysia
1030 Bruxelles Fax +7.812.327.53.23 2179 Dunwin Drive #4 Sartorius Malaysia Sdn. Bhd
Mississauga, ON L5L 1X2 Lot L3-E-3B, Enterprise 4
Phone +32.2.756.06.90 Technology Park Malaysia
Fax +32.2.481.84.11 Spain & Portugal Phone +1.905.569.7977 Bukit Jalil
Sartorius Spain, S.A. Toll-Free +1.800.668.4234 57000 Kuala Lumpur, Malaysia
Avda. de la Industria, 32 Fax +1.905.569.7021
Finland & Baltics Phone +60.3.8996.0622
Edificio PAYMA
Sartorius Biohit Liquid Handling Oy Fax +60.3.8996.0755
28108 Alcobendas (Madrid)
Laippatie 1 Mexico
00880 Helsinki Phone Spain +34.913.586.095 Sartorius de Mxico, S.A. de C.V.
Phone Portugal +351.800.855.800 Libramiento Norte de Tepotzotlan s/n, Singapore
Phone +358.9.755.951 Fax Spain +34.913.589.623 Sartorius Singapore Pte. Ltd
Colonia Barrio Tlacateco,
Fax +358.9.755.95.200 Fax Portugal +351.800.855.799 1 Science Park Road,
Municipio de Tepotzotlan,
Estado de Mxico, The Capricorn, #05-08A,
C.P. 54605 Singapore Science Park II
Hungary Switzerland Singapore 117528
Sartorius Hungria Kft. Sartorius Mechatronics Switzerland AG Phone +52.55.5562.1102
Kagyl u. 5. Fax +52.55.5562.2942 Phone +65.6872.3966
Ringstrasse 24a
2092 Budakeszi Fax +65.6778.2494
8317 Tagelswangen (ZH) [email protected]
Phone +3623.457.227 Phone +41.44.746.50.00
Fax +3623.457.147 Fax +41.44.746.50.50 South Korea
Peru
Specifications subject to change without notice. Printed in the EU on paper bleached without chlorine. | W
Sartorius Korea Ltd.
Sartorius Peru S.A.C. 8th Floor, Solid Space B/D,
Ireland
U.K. Av. Emilio Cavenecia 264 San Isidro PanGyoYeok-Ro 220, BunDang-Gu
Sartorius Ireland Ltd.
Sartorius UK Ltd. 15073 Lima, Per SeongNam-Si, GyeongGi-Do, 463-400
Unit 41, The Business Centre
Stadium Business Park Longmead Business Centre Phone +51.1.441 0158 Phone +82.31.622.5700
Ballycoolin Road Blenheim Road, Epsom Fax +51.1.422 6100 Fax +82.31.622.5799
Dublin 11 Surrey KT19 9QQ
Phone +353.1.8089050 Phone +44.1372.737159
Publication No.: SL-1530-e161016 Order No.: 85034-538-81 Ver. 10 | 2016
Fax +353.1.8089388 Fax +44.1372.726171
Asia | Pacific Thailand
Sartorius (Thailand) Co. Ltd.
129 Rama 9 Road,
Ukraine Australia Huaykwang
LLS Sartorius RUS Sartorius Australia Pty. Ltd. Bangkok 10310
Post Box 440 B Unit 5, 7-11 Rodeo Drive
Dandenong South Vic 3175 Phone +66.2643.8361-6
01001 Kiev, Ukraine
Fax +66.2643.8367
Phone +380.44.411.4918 Phone +61.3.8762.1800
Fax +380.50.623.3162 Fax +61.3.8762.1828
China
Sartorius (Shanghai) Trading Co., Ltd.
3rd Floor, North Wing, Tower 1
No. 4560 Jinke Road
Zhangjiang Hi-Tech Park
Pudong District
Shanghai 201210, P.R. China
Phone +86.21.6878.2300
Fax +86.21.6878.2882
www.sartorius.com