ICS 2000 Manual
ICS 2000 Manual
ICS 2000 Manual
OPERATORS MANUAL
1 Introduction
1.1 Introduction to Ion Chromatography (IC) . . . . . . . . . . . . . . . . . . . . . . .1-1
1.2 Overview of the ICS-2000 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-4
1.3 About This Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-5
1.3.1 Safety Messages and Notes . . . . . . . . . . . . . . . . . . . . . . . . . . .1-6
1.3.2 Safety Labels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-8
2 Features
2.1 Operating Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1
2.1.1 Front Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1
2.1.2 Top Cover . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-6
2.1.3 Component Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-7
2.1.4 Rear Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-10
2.2 Flow Schematic . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-12
2.3 The Chromeleon Interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-14
2.3.1 The Chromeleon Main Window and Browser . . . . . . . . . . . .2-15
2.3.2 The ICS-2000 Control Panels . . . . . . . . . . . . . . . . . . . . . . . . .2-16
2.4 System Component Details . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-22
2.4.1 Vacuum Degas Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . .2-22
2.4.2 Eluent Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-24
2.4.3 Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-24
4 Troubleshooting
4.1 Alarms and Error Conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-3
4.2 Liquid Leaks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-13
4.3 Pump Difficult to Prime or Loses Prime . . . . . . . . . . . . . . . . . . . . . . .4-15
4.4 Pump Does Not Start . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-17
4.5 No Flow . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-18
4.6 Erratic Flow/Pressure Reading . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-20
4.7 Excessive System Backpressure . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-20
4.8 Peak Ghosting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-21
4.9 Nonreproducible Peak Height or Retention Time . . . . . . . . . . . . . . . .4-22
4.10 Abnormal Retention Time or Selectivity . . . . . . . . . . . . . . . . . . . . . .4-22
4.11 No Cell Response . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-23
4.12 High Cell Output . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-23
4.13 Baseline Noise or Drift . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-24
5 Service
5.1 Diagnostic and Calibration Procedures . . . . . . . . . . . . . . . . . . . . . . . . 5-1
5.1.1 Chromeleon Wellness Panel Overview . . . . . . . . . . . . . . . . . . 5-2
5.1.2 Diagnostic and Calibration Touch Screen Overview . . . . . . . 5-4
5.1.3 Calibrating the Conductivity Cell . . . . . . . . . . . . . . . . . . . . . . 5-5
5.1.4 Calibrating the Flow Rate . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-8
5.1.5 Calibrating the Vacuum Degas Assembly . . . . . . . . . . . . . . . 5-10
5.2 Isolating a Restriction in the Liquid Lines . . . . . . . . . . . . . . . . . . . . . 5-11
5.3 Replacing Tubing and Fittings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-14
5.4 Rebuilding the Injection Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-15
5.5 Cleaning and Replacing the Pump Check Valves . . . . . . . . . . . . . . . 5-16
5.6 Replacing a Pump Piston Seal and Piston Rinse Seal . . . . . . . . . . . . 5-18
5.7 Replacing a Pump Piston . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-22
5.8 Replacing the Waste Valve or Priming Valve O-Ring . . . . . . . . . . . . 5-23
5.9 Replacing the Conductivity Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-25
5.10 Replacing the Suppressor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-28
5.11 Replacing the Column Heater . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-29
5.12 Replacing the Column Heater Heat Exchanger . . . . . . . . . . . . . . . . . 5-32
5.13 Replacing the Eluent Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-33
5.14 Replacing the Leak Sensor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-35
5.15 Priming with Isopropyl Alcohol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-36
5.16 Changing Main Power Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-37
A Specifications
A.1 Electrical . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A-1
A.2 Physical . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A-1
A.3 Environmental . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A-1
A.4 Front Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A-2
A.5 Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A-2
A.6 Detector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A-3
A.7 Conductivity Cell with Heat Exchanger . . . . . . . . . . . . . . . . . . . . . . . A-3
A.8 Injection Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A-3
A.9 Vacuum Degas Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A-3
A.10 Column Heater . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A-4
A.11 Eluent Generator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A-4
B Installation
B.1 Facility Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . B-1
B.2 Unpacking the ICS-2000 System . . . . . . . . . . . . . . . . . . . . . . . . . . . . B-2
B.2.1 Unpacking the Computer (North America only). . . . . . . . . . . B-4
B.2.2 Unpacking the Computer (outside North America) . . . . . . . . B-4
B.3 Installing Chromeleon . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . B-5
B.4 Installing the Chromeleon Software License . . . . . . . . . . . . . . . . . . . B-6
E Reordering Information
F FAQ
F.1 How do I hook up an autosampler? . . . . . . . . . . . . . . . . . . . . . . . . . . . F-1
F.2 How do I print? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . F-1
F.3 Why are the retention times moving? . . . . . . . . . . . . . . . . . . . . . . . . . F-1
F.4 How do I adjust retention times? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . F-1
F.5 When should I remake standards? . . . . . . . . . . . . . . . . . . . . . . . . . . . . F-2
F.6 When should I replace the eluent generator cartridge? . . . . . . . . . . . F-2
F.7 How do I start Chromeleon? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . F-2
F.8 How do I delete data? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . F-2
F.9 How do I back up data? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . F-2
F.10 How do I shut off the system? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . F-2
G Glossary
6. Data Analysis
Conductivity
Cell 5. Detection
Suppressor
Eluent
4. Suppression
1. Eluent
Separator
Delivery Column
3. Separation
Pump Guard Column
Injection
Valve
Sample
2. Sample
Injection
Sample Loop
1. Eluent Delivery
Eluent, a liquid that helps to separate the sample ions, carries the
sample through the ion chromatography system. The ICS-2000
includes an eluent generator, which generates eluent online from
deionized water.
When the ICS-2000 is controlled from the front panel, only isocratic
eluent delivery is possible. This means that the eluent composition
and concentration remain constant throughout the run. Gradient
delivery (a change in concentration over time) is possible when the
ICS-2000 is controlled by Chromeleon (the data collection system
for the ICS-2000).
2. Sample Injection
The liquid sample is loaded into a sample loop either manually or
automatically (if an automated sampler is installed). When triggered,
the ICS-2000 injects the sample into the eluent stream.
The pump pushes the eluent and sample through the guard and
separator columns (chemically-inert tubes packed with a polymeric
resin). The guard column removes contaminants that might poison the
separator column.
3. Separation
As the eluent and sample are pumped through the separator column,
the sample ions are separated. In the ICS-2000, the mode of
separation is called ion exchange. This is based on the premise that
different sample ions migrate through the IC column at different rates,
depending upon their interactions with the ion exchange sites.
4. Suppression
After the eluent and sample ions leave the column, they flow through
a suppressor that selectively enhances detection of the sample ions
while suppressing the conductivity of the eluent.
5. Detection
A conductivity cell measures the electrical conductance of the sample
ions as they emerge from the suppressor and produces a signal based
on a chemical or physical property of the analyte.
6. Data Analysis
The conductivity cell transmits the signal to a data collection system.
The data collection system (for the ICS-2000, this is Chromeleon)
identifies the ions based on retention time, and quantifies each analyte
by integrating the peak area or peak height. The data is quantitated by
comparing the sample peaks in a chromatogram to those produced
from a standard solution. The results are displayed as a chromatogram
and the concentrations of ionic analytes can be automatically
determined and tabulated.
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pourrait entraner des blessures mineures modres. galement
utilis pour signaler une situation ou une pratique qui pourrait
gravement endommager l'instrument mais qui n'entranera pas de
blessures.
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Notes
Alternating current
Power supply is on
This chapter describes key ICS-2000 features and introduces the Chromeleon user
interface.
Injection Port
The sample to be analyzed can
be injected manually into the
injection port, using a syringe.
For automated sample
injection, the ICS-2000 must be
connected to an autosampler.
For more information about
Touch
sample injection, see
Screen
Section 3.10.
Power
Touch Screen
LED
The LCD touch screen provides
local control of most ICS-2000
functions. You can control
many of these functions
Injection
Port
directly on the touch screens
HOME page (see Figure 2-2).
From the HOME page you can
also go to a series of pages
where you can access all other
locally controlled ICS-2000
operating functions.
Figure 2-1. ICS-2000 Front Panel
Status Field
Edit Field
Command
Button
(selected)
Command
Button
(unselected)
NOTE To adjust the screen contrast, open the front door and
adjust the knurled knob under the screen (see
Figure 2-8).
Touch CANCEL
to cancel the
entry and return
Touch a
to the previous
number to
page.
enter the
value. Touch ENTER to
confirm the
entry and return
to the previous
page.
Touch a page
name to display
the page.
Touch to
select a
different
option.
Touch to
return to the
Home page.
Touch an
option to
select it.
Tubing Chase
The tubing chase under the EGC and CR-TC connectors routes tubing
from the eluent reservoir and EGC holder to the front of the ICS-2000.
EGC Service
The EGC service area holds the cartridge during installation and
replacement. See Section B.13 for instructions.
11
10
1 Eluent Valve
9
2 Leak Sensor
6 3 Pump Heads
7
4 Pressure Transducer
8
5 Injection Valve
6 Column Heater
and Columns
7 Suppressor
8 DS6 Cell
5
4
9 Mounting brackets for
a second suppressor
10 Screen Adjustment
Knob (under display)
3
2
11
1
Eluent Valve
The eluent valve controls the flow from the eluent reservoir. It opens
automatically when the pump is running and closes when the pump is off.
Leak Sensor
The leak sensor is installed in the drip tray at the bottom of the component
panel. If liquid accumulates in the tray, an error message is reported in
Chromeleon on the LCD touch screen.
Pump Heads
The ICS-2000 includes a serial isocratic dual-head pump. The flow rate
can be set to 0.00 and to between 0.05 and 5.0 mL/min. However, for
optimum performance, set the flow rate to between 0.4 and 2.0 mL/min.
Setting the flow rate to 0.00 turns off the pump. See Section 2.4.3 for
details about the pump.
Pressure Transducer
Injection Valve
Both the separator and guard columns are packed with resin and perform
the separation of the sample ions. The main function of the guard column
is to trap contaminants and remove particulates that might damage the
separator column.
Column Heater
The column heater controls the temperature of the separator and guard
columns. The temperature can be set to between 30 C and 60 C and it
must be set at least 5 C above the ambient temperature. See Section 2.4.6
for details about the column heater.
Suppressor
The suppressor reduces the eluent conductivity and enhances the
conductivity of the sample ions, thereby increasing detection sensitivity.
Either an AES Atlas Electrolytic Suppressor, SRS Self-Regenerating
Suppressor, or MMS MicroMembrane Suppressor can be used with
the ICS-2000. See Section 2.4.7 for details.
The upper tubing chase routes tubing from the top cover to the component
panel. The lower tubing chase routes tubing from the component panel
through the ICS-2000 interior to the rear panel.
Tubing Clips
Analog Output
Connector
Power
Switch
USB
Connections
Waste Lines
USB Connections
A USB receptacle is provided to allow connection to the Chromeleon
computer. Two USB ports are provided for connecting to other USB
devices. See Section B.5 for connection instructions.
Tubing Clips
The tubing clips hold tubing in place when routing it from the top cover.
Power Switch
The power switch provides on/off control of power to the ICS-2000.
The power supply cord is used as the main disconnect device. Make
sure the socket-outlet is located near the ICS-2000 and is easily
accessible.
1 EGC
DEIONIZED 6 OUT
WATER
OUT
IN OUT
OUT
PUMP 17
DEGAS EGC
8 DEGAS
SUPPRESSOR
IN OUT
HEAT EXCHANGER
13
CR-TC 14 IN
7 COND OUT
SEPARATOR COLUMN
16 CELL
15
COLUMN HEATER
PULSE IN
DAMPER
5 11
SAMPLE
IN
S L OUT
10 W 9 C
4 12
GUARD
L P PRESS.
XDUCER.
VALVE
IN
WASTE
WASTE
18
OUT OUT
3
PUMP PUMP
HEAD HEAD
IN IN
WASTE
IN
2 OUT
ELUENT
VALVE
Deionized water from the reservoirn flows first through the pump degas
assembly and then through the eluent valveo to the pumpp. It is then pushed
through the pressure transducerq , which measures the system pressure. From
there, the water flows through a pulse damperr, which smooths minor
pressure variations from the pump to minimize baseline noise.
Water then flows into the EluGen cartridge (EGC)s , which generates the
programmed concentration of eluent. Eluent exits the cartridge and flows
through the CR-TCt (which traps ionic contaminants), through the EGC
degas tubing assemblyu, and on to the injection valvev.
After sample is loaded into the sample loopw and the injection valve is
toggled to the Inject position, eluent passes through the sample loop.
The eluent/sample mixture is pumped through the heat exchanger 11 , which
heats the mixture to the column heater temperature. The mixture then goes to
the guard and separator columns 12 and through the suppressor 13 .
From the suppressor, the mixture flows through the cell 14 , where the analytes
are detected. A digital signal is sent to Chromeleon software. Analog output
can be collected simultaneously.
The mixture flows out of the cell and is recycled back into the suppressor 15 ,
where it is the water source for the regenerant chamber.
Regenerant waste from the suppressor is directed back to the CR-TC 16 , and
then to the EGC degas tubing 17 , where any released hydrogen or oxygen gas
is removed before it is sent to waste 18 .
System Control Panels for the ICS-2000 are located in the Dionex
Templates/Panels/Dionex_IC\ICS-2000 folder. Chromeleon provides
panels for each ICS-2000 system configuration. There are two styles of
panels:
Traditional system panels (see Figure 2-12) provide controls on a
single window with minimal graphics.
System panels (see Figure 2-13) provide a more graphical interface
than the traditional style. On these panels, clicking a control often
brings up another window in which you enter a value.
When you open a System Control Panel, select a panel that matches your
system configuration and choose the style that you prefer.
Datasource
Displays the database in which the chromatographic data for the sample
is saved.
Sequence
Displays the sequence in which this sample is included.
Sample
Displays the name of the sample that is running.
Program
Displays the name of the program that is running.
Elapsed Time
Displays the time that has elapsed since the sample analysis began.
Run Time
Displays the total run time.
System
System Startup
Starts the pump, suppressor, and eluent generator. The flow rate,
suppressor current, and eluent concentration settings that were in effect
at system shutdown (see below) are restored.
System Shutdown
Stops the pump, suppressor, and eluent generator. The flow rate,
suppressor current, and eluent concentration settings currently in effect
are remembered and will be restored at system startup (see above).
Audit Trail
The Audit Trail displays an account of every event that occurs during
ICS-2000 operation. This includes errors, status messages, operational
events, etc.
Pump
Eluent Bottle
The entry field and gauge indicate the level
of liquid (in liters) in the eluent bottle. This
is a user controlled feature. The level must
be entered by the user when the reservoir is
filled. See Section 3.4.2 for details.
Connected
Connects the ICS-2000 to the Chromeleon
Control Panel, enabling communication
between the PC and the module. Clearing
the check box disconnects the ICS-2000
from the PC.
Hold Mode
Indicates when an automatic (batch) run is on hold; pump flow is not
stopped.
Stop Mode
Indicates when an automatic (batch) run is stopped; pump flow is also
stopped.
Flow Rate
Sets the pump flow rate in mL/min.
Pressure
The sliders select the upper and lower pressure limits. The Pressure field
displays the current pressure.
On, Off, Prime
Turn the pump on and off or start the pump priming function.
Inject Valve
Displays the current position of the inject
valve. The Inject and Load buttons select the
position.
Eluent Flow Valve
Displays the current position of the eluent
flow valve. The Closed and Open buttons
select the position.
Column Heater
Set Temp
Sets the temperature of the column heater.
Detector
Connected
Connects the ICS-2000 to the Chromeleon Control Panel, enabling
communication between the PC and the module. Clearing the Connected
check box disconnects the ICS-2000 from the PC.
Autozero
Sets the conductivity to zero.
Ready
Indicates if the detector is ready to begin data acquisition.
Signal
Displays the offset conductivity.
Data Rate
Sets the rate at which data points are recorded.
Supp. Type
Selects the type of suppressor installed.
SRS Current
Sets the current supplied to the suppressor.
Cell Temp
Sets the temperature of the cell.
SRS Mode
Selects whether the suppressor is on or off.
Show Plot
Displays the ICS-2000 detector signal plot in a separate window.
under the timebase, and select Properties from the Edit menu. Select the
Options tab (see Figure 2-14).
2.4.3 Pump
The ICS-2000 pump is a microprocessor-based isocratic eluent delivery
system. Its variable speed, dual-piston series design ensures pulse-free
pumping for the most demanding applications.
The primary pump head pumps mobile phase into the secondary head (see
Figure 2-16). The check valves, which prevent reverse flow through the
pump, are located on the bottom (inlet) and top (outlet) of the primary
pump head. The priming valve is on the front of the pump head.
Outlet Check
Pressure Valve
Transducer
Primary
Pump Head
Secondary
Pump Head Priming
Valve
Inlet Check
Waste Valve Valve
To open the priming valve, turn the knob one-quarter to one-half turn
counterclockwise. When the priming valve is open, liquid can flow into
and out of the primary pump head via the port in the front of the valve.
Pressure Transducer
Pulse Damper
Flow output from the pressure transducer continues to the pulse damper,
which smooths minor pressure variations. From there, flow is directed to
the injection valve and then to the remainder of the chromatography
system.
The pump includes a piston seal wash assembly that can be set up to
continuously rinse the back of the piston seals to remove salt crystals and
prolong the life of the seals. To use this feature, an external water source
must be connected. See Section B.22 for connection instructions.
For continued protection of the pump, replace the piston rinse seals (see
Section 5.6) and O-rings in the seal wash assembly every 6 months, or
whenever you replace the main piston seals for the ICS-2000 pump.
To Column To Column
Sample Sample
Loop Loop
Eluent flows through either the Load or Inject path, depending on the
valve position.
In the Load position, sample is loaded into the sample loop, where it
is held until injection. Eluent flows from the pump, through the valve,
and to the column, bypassing the sample loop. Sample flows from the
syringe or automated sampler line (if installed), through the valve,
and into the sample loop. Excess sample flows out to waste.
In the Inject position, sample is swept to the column for analysis.
Eluent flows from the pump, through the sample loop, and on to the
column, carrying the contents of the sample loop with it. Section 3.10
describes how to inject samples.
Figure 2-18 shows the injection valve connections. The injection valve is
plumbed at the factory with all tubing and fittings for connection to the
pump, injection port, column, and waste. A 25-L PEEK sample loop
(P/N 042857) is installed between ports L (1) and L (4). Dionex offers
2.4.7 Suppressor
The suppressor reduces the eluent conductivity and enhances the
conductivity of the sample ions, thereby increasing detection sensitivity.
Either an Atlas Electrolytic Suppressor, Self-Regenerating Suppressor, or
MicroMembrane Suppressor can be used with the ICS-2000.
For details about any of the suppressors or for information about selecting
a suppressor for your application, refer to the suppressor manuals. The
manuals are on the Dionex Reference Library CD-ROM (P/N 053891),
located in the ICS-2000 Ship Kit (P/N 057905).
Temperature Control
Temperature Compensation
The cell front cover provides CELL IN and CELL OUT fittings for
connecting the cell to the suppressor (see Figure 2-8). The remaining cell
components are mounted behind the component panel. To replace, the cell
remove the screws on the cell front cover and pull the entire cell assembly
out through the component panel. See Section 5.9 for cell replacement
instructions.
This chapter describes routine operating and maintenance procedures for the ICS-
2000 Ion Chromatography System.
Start Chromeleon
Check all
connections
Set operating
conditions
Equilibrate the
system
Prepare samples
Verify operating
status
Process samples
1. If the Chromeleon Server is not running (the icon on the taskbar is crossed out
in red ), start the Server by right-clicking the icon and selecting Start
Server. When the server starts, the icon changes to gray .
Install the end-line filter on the end of the deionized water line, inside the
reservoir. Verify that the end of the filter extends to the bottom of the
reservoir and that the filter is submerged in deionized water. This prevents
air from being drawn through the lines. Refer to the Pressurizable
Reservoir Installation Instructions for details.
3. Make sure the CR-TC electrical cable (black) is connected to the CR-TC
connector.
4. Make sure the USB cable from the computer is connected to the USB
receptacle on the ICS-2000 rear panel.
1. Verify that the priming valve on the primary pump head (see Figure 3-11) is
closed (turned all the way clockwise).
2. Open the waste valve on the secondary pump head by turning the knob one-
quarter to one-half turn counterclockwise. Opening the valve directs the
eluent flow path to waste and eliminates backpressure.
Secondary
Pump Head
Waste Valve
(open)
Primary
Pump Head
Priming Valve
(close)
3. Press Prime on the Chromeleon Control Panel or the ICS-2000 touch screen
HOME page. A confirmation message is displayed, asking if you opened the
waste valve. Select Yes. The pump will begin pumping at approximately
3.0 mL/min.
4. Continue priming the ICS-2000 until all air and previous eluent are purged
and no air bubbles are exiting the waste line.
5. Press Pump Off.
6. Close the waste valve. Do not overtighten. The pump is now ready for
operation.
Set or verify system operating parameters from either the Chromeleon Control
Panel or from the touch screen HOME page. Operating parameters can also be set
automatically by loading a Chromeleon sequence.
1. Verify that the pump is on and set to the correct flow rate.
2. Verify that the suppressor current is on and that the setting is correct.
3. Verify that the eluent generator is turned on and the correct concentration is
set. Verify that the CR-TC is turned on.
4. Verify that the cell heater is set to the correct value.
5. Verify that the column heater is set to the correct value.
stand-alone operation. This requires a TTL cable connection between the AS50
and the ICS-2000. See Section D.3 for stand-alone setup instructions.
2. Fill the 1 cc syringe (P/N 016388) provided in the ICS-2000 Ship Kit
(P/N 057905) with a calibration standard or sample.
3. Insert the syringe into the injection port on the ICS-2000 front door.
4. Verify that the injection valve is in the Load position.
5. Overfill the sample loop with several sample loop volumes. Excess
sample will exit through the injection valve waste line.
6. Leave the syringe in the port.
7. Switch the injection valve to the Inject position (see Section 3.10.4).
NOTE When you run samples manually from the front panel
(without using Chromeleon), the data is saved only
temporarily in the ICS-2000 memory. For a permanent
record of the data, connect a recording device (chart
recorder, integrator, etc.) to the analog output. See
Section B.20 for analog output connection instructions.
7. If you are controlling the ICS-2000 from the touch screens, select the
PLOT button on the HOME page to view a plot of the conductivity
data.
3. To start the sample processing, make sure the System Control Panel is
open (see Section 3.3) and is the active window. Then, select Edit
from the Batch menu. The Batch dialog box opens (see Figure 3-13).
4. Click the Add button on the Batch List tab and add the sequence you
created in Step 2. See Figure 3-14 for an example.
Figure 3-14. Batch Dialog Box with an Example Sequence Added to the Batch List
3.12 Maintenance
This section describes routine maintenance procedures that users may perform.
All other maintenance procedures must be performed by Dionex personnel.
As Needed
Regularly check the eluent reservoir to see if it needs to be refilled.
Daily
Check the ICS-2000 component mounting panel (see Figure 2-8) for leaks or
spills. Wipe up spills. Isolate and repair leaks (see Section 4.2). Rinse off any
dried eluent with deionized water.
Check the waste container daily and empty when needed.
Weekly
Once a week, check fluid lines for crimping or discoloration. Relocate any
pinched lines. Replace damaged lines.
Check the junctions between the pump heads and the pump casting for
evidence of liquid leaks. If piston seal wash tubing is not connected, check the
drain tubes at the rear of the pump heads for evidence of moisture. Normal
friction and wear may gradually result in small liquid leaks around the piston
seal. If unchecked, these leaks can gradually contaminate the piston housing,
causing the pump to operate poorly. If leaks occur, replace the piston seals
(see Section 5.6).
Check the end-line filter (P/N 045987) and change if needed. When new, end-
line filters are pure white. If the system is in continuous operation, change the
end-line filter weekly, or whenever it becomes discolored. Replace the filter
more often if bacterial buildup is visible or if the eluent does not contain
solvent.
Yearly
Rebuild the injection valve (see Section 5.4).
Replace the AS40 Automated Sampler tip and tubing. The AS40/ASM
Maintenance Kit (P/N 055647) contains all of the components required to
replace the sampling tip and the tubing between the tip and the injection
valve. Instructions are included with the kit.
Perform the AS50 preventive maintenance procedure. The AS50 Maintenance
Kit (P/N 060581) contains all of the required components and instructions for
performing the procedure.
This error occurs when the column heater temperature is higher than the
maximum allowed. This error may occur if the ICS-2000 is operating in an
extreme environment (greater than 40 C).
To troubleshoot:
Refer to Section A.3 for environmental specifications.
This error message occurs when the column heater is unplugged from the
component panel.
To troubleshoot:
1. Check that the column heater is correctly plugged into the component
panel.
2. If the error persists, the column heater may be faulty. Replace the
column heater (see Section 5.11).
This error occurs when there is a short circuit on the thermistor input used to
measure the column heater temperature. This error indicates a faulty column
heater.
To troubleshoot:
Replace the column heater (see Section 5.11).
This error occurs when the current applied to the CR-TC exceeds the
maximum current allowed.
To troubleshoot:
1. Check the CR-TC cable connection.
2. If the error persists, contact Dionex for assistance. There may be a
malfunction in the CR-TC control electronics.
This error appears normally when you click the Shutdown button on the
Chromeleon Control Panel. The message also appears when you turn off the
pump flow while the EGC current (and CR-TC) is on. The CR-TC is
automatically turned off.
To troubleshoot:
If the pump stopped unexpectedly, see Section 4.5 for pump
troubleshooting information.
This error occurs when the degas vacuum does not reach the expected level
during calibration.
To troubleshoot:
1. Retry the calibration.
2. If calibration still fails, the vacuum degas assembly may need to be
replaced. Contact Dionex for assistance.
This error occurs if the measured current is outside the expected range when
running the eluent generator calibration procedure.
To troubleshoot:
Review the procedure to verify you have set up the ICS-2000 correctly
and then rerun the calibration.
If the error re-occurs, contact Dionex for assistance.
The maximum eluent concentration that can be set for a particular application
depends on the suppressor type, the EGC type, and the flow rate. This error
occurs if the selected concentration is too high for the current flow rate.
To troubleshoot:
Refer to the column manual, which is included on the Dionex Reference
Library CD-ROM (P/N 053891), for the concentration of eluent to use
with your application. The CD-ROM is located in the ICS-2000 Ship Kit
(P/N 057905). Reset the eluent concentration and/or the flow rate to the
recommended values.
This error occurs if the flow rate is set to a value not supported by the
eluent generator. The ICS-2000 allows a flow rate range of 0.00 to
5.00 mL/min without an eluent generator. With an eluent generator, the
allowed range is 0.10 to 3.00 mL/min.The recommended operating range is
0.4 to 2.00 mL/min.
To troubleshoot:
Set the flow rate to a value within the allowed range.
EGC-1 OVER-CURRENT
This error occurs when the current applied to the eluent generator cartridge
exceeds the maximum current allowed. The EGC current is turned off to
prevent damage to the EGC. This error may occur if the liquid flow to the
cartridge is interrupted.
To troubleshoot:
See Section 4.5 for troubleshooting no flow situations.
EGC-1 OVER-VOLTAGE
This error occurs when the current applied to the eluent generator cartridge
exceeds the maximum current allowed. The EGC current is turned off to
prevent damage to the EGC. This error may occur if the liquid flow to the
cartridge is interrupted.
To troubleshoot:
See Section 4.5 for troubleshooting no flow situations.
The leak sensor is installed in the drip tray at the bottom of the component
panel (see Figure 2-8). If liquid accumulates in the tray, the sensor signals the
problem and the Leak sensor wet error message appears.
To troubleshoot:
1. Locate the source of the leak by visually inspecting the tubing,
fittings, and components on the component panel.
2. Tighten fittings or replace tubing and fittings as required. Refer to
Section 4.2 for detailed troubleshooting of various types of leaks.
3. After fixing the leak, dry the drip tray thoroughly to prevent the leak
sensor from triggering additional error messages.
If the injection valve fails to switch position within 1 second of being toggled,
the ICS-2000 Moduleware reports an error to Chromeleon and the
Load/Inject valve error message appears.
To troubleshoot:
1. If a sequence is being executed, terminate the sequence by selecting
Stop on the Chromeleon Batch menu.
2. Turn off the ICS-2000 power briefly and then restart.
3. Try to toggle the valve from Load to Inject by clicking the Load and
Inject buttons on the ICS-2000 Control Panel in Chromeleon or on
the touch screen HOME page.
4. If the problem persists, contact Dionex for assistance.
This error occurs if you try to calibrate the pressure slope when the pressure is
less than 3 MPa (500 psi).
To troubleshoot:
This is a service level error message. Contact Dionex for assistance.
The ICS-2000 has a maximum system pressure limit of 34.5 MPa (5000 psi).
When the eluent generator cartridge is configured in Chromeleon (see
Section B.13.5), the maximum system pressure limit is reduced to 20.6 MPa
(3000 psi). The pressure limit can also be set on the touch screen PUMP page
(see Section C.7). If the system pressure exceeds the set limit for at least
0.5 seconds, the pump stops and the Pump over pressure error message
appears.
To troubleshoot:
1. Check for blockages in the liquid lines by working your way
backward from the cell to the pump. See Figure 2-10 for the flow
schematic).
2. Check that the flow rate is set to the correct value.
3. Check that the high pressure limit is set to the correct value.
4. Restart the pump.
The ICS-2000 has a minimum pressure limit of 0. When the eluent generator
cartridge is configured in Chromeleon (see Section B.13.5), the low pressure
limit is raised to 1.4 MPa (200 psi). The pressure limit can also be set on the
touch screen PUMP page (see Section C.7). If the system pressure falls below
the low pressure limit, the pump stops and the Pump under pressure error
message appears.
To troubleshoot:
1. Make sure the eluent reservoir is full.
2. Check for liquid leaks (see Section 4.2).
3. Check that the waste valve is closed.
4. Prime the pump (see Section B.14).
5. Restart the pump.
This error appears if you turn on the suppressor and the ICS-2000 cannot
establish a connection with the suppressor.
To troubleshoot:
1. Check the suppressor cable connection (see Section 5.10).
2. If the error persists, replace the suppressor (see Section 5.10).
This error appears when, in order to maintain the selected current, the ICS-
2000 is required to apply a higher voltage than the suppressor can support.
To troubleshoot:
Reduce the flow rate.
Rehydrate the suppressor. Refer to the suppressor manual for instructions.
Suppressor manuals are included on the Dionex Reference Library CD-
ROM (P/N 053891), located in the ICS-2000 Ship Kit (P/N 057905).
If the error persists, replace the suppressor (see Section 5.10).
This error appears if you turn on the suppressor and the ICS-2000 cannot
establish a connection with the suppressor.
To troubleshoot:
1. Check the suppressor cable connection (see Section 5.10).
2. If the error persists, contact Dionex for assistance. There may be a
problem in the suppressor controller electronics. The ICS-2000
electronics components cannot be serviced by the user.
This error appears normally when you click the Shutdown button on the
Chromeleon Control Panel. The message also appears anytime you turn off
the pump flow while the suppressor is on. The suppressor is automatically
turned off to prevent damage to the suppressor.
To troubleshoot:
If the pump stopped unexpectedly, see Section 4.5 for pump
troubleshooting information.
3. Verify that the Degas check box is enabled under Installed Options.
4. Check the Degas Mode settings. Select either the Monitor option or
the Cycle option. If you select Cycle, set the time on to 30 seconds
and the time off to 10 minutes. If Cycle is already selected, try
increasing the time on or decreasing the time off.
NOTE Degas Mode options can also be selected from the touch
screen PUMP page (see Section C.7).
4.5 No Flow
Pump waste or priming valve open (see Figure 4-2).
Close the valves by turning the knobs clockwise until fingertight. DO NOT
OVERTIGHTEN! Overtightening may damage the valve and the pump
head.
Priming Valve
Waste Valve
Incorrect eluent
Check the EGC concentration setting.
Inadequate system backpressure
Add backpressure tubing between the injection valve and the ELUENT OUT
port on the EGC to maintain a pressure of 16 MPa 0.7 MPa (2,300 psi
100 psi). See Section B.13.6 for details.
Rapid changes in ambient temperature
Make sure the ambient temperature is between 4 and 40 C (40 and 104 F).
Make sure air conditioning and heating vents are directed away from the ICS-
2000 and the ICS-2000 front door is closed.
Insufficient system equilibration following changes to operating
parameters; especially apparent when operating at high sensitivities
Allow a longer system equilibration time (up to 2 hours) before starting
operation.
Incorrect suppressor operating conditions
Refer to the suppressor manual for troubleshooting information. Suppressor
manuals are included on the Dionex Reference Library CD-ROM
(P/N 053891), located in the ICS-2000 Ship Kit (P/N 057905).
Cell above or below temperature
Contact Dionex for assistance.
Damaged piston seal
Replace the piston seal (see Section 5.6).
b. Under Installed Options, verify that the Degas check box is selected.
c. Under Degas Mode, verify that the Always Off option is not selected. If
it is, select one of the following settings:
Cycle, and select On: 30 seconds and Off: 10 minutes
Monitor
d. If the Degas Mode settings are correct, but the degas assembly still does
not run, test the assembly by selecting the Always On option. The pump
should turn on immediately. If it does not, the vacuum degas assembly
may need to be replaced. Contact Dionex for assistance.
NOTE Degas Mode options can also be selected from the touch
screen PUMP page (see Section C.7).
Select the Degas Mode, Always On option only when testing the vacuum
degas assembly. Do not select the Always On option for routine use.
e. If the degas pump runs when you select Always On, but does not run
when Monitor mode is selected, recalibrate the degas assembly (see
Section 5.1.5).
This chapter describes ICS-2000 service and repair procedures that the user can
perform. All procedures not included here, including electronics-related repair
procedures, must be performed by Dionex personnel. For assistance, contact
Dionex Technical Support. In the U.S., call 1-800-346-6390. Outside the U.S.,
call the nearest Dionex office.
Before replacing any part, refer to the troubleshooting information in Chapter 4 to
isolate the cause of the problem.
Substituting non-Dionex parts may impair ICS-2000 performance,
thereby voiding the product warranty. Refer to the warranty statement
in the Dionex Terms and Conditions for more information.
10. Select 1.00 mL/min to begin pumping 1.0 mM KCl through the cell at
1.0 mL/min.
11. Wait until the total conductivity reading stabilizes (in about
15 minutes) and then select Calibrate on the Wellness Panel or the
ICS-2000 touch screen.
After calibration, the conductivity reading should be 147.00 2 S
and the cell constant should be between 120 and 180. If this is not the
case, contact Dionex for help.
12. If you are using the Wellness Panel, click Log to record the new
calibration values in the Audit Trail.
13. If you are using the ICS-2000 touch screen, record the new
calibration values in the Chromeleon Wellness database. To do this,
open the Wellness Panel (see Section 5.1.1) and click Upload under
Update Wellness Database.
14. Flush the KCl solution from the system by pumping deionized water
through the cell. When the conductivity drops to less than 1 S/cm,
stop the pump.
15. Reconnect the pump to the injection valve and reconnect the line
from the suppressor to the cell inlet.
Items Needed
To Calibrate
If the restriction has caused such high pressure that the system cannot be
operated, you must work forward through the flow schematic, adding parts
one at a time until an abnormal pressure increase (and hence, the restriction) is
found.
3. If the restriction is in the tubing or fitting, remove the restriction by back
flushing or by replacing the tubing or fitting (see Section 5.3).
1 EGC
DEIONIZED 6 OUT
WATER
OUT
IN OUT
OUT
PUMP 17
DEGAS EGC
8 DEGAS
SUPPRESSOR
IN OUT
HEAT EXCHANGER
13
CR-TC 14 IN
7 COND OUT
SEPARATOR COLUMN
16 CELL
15
COLUMN HEATER
PULSE IN
DAMPER
5 11
SAMPLE
IN
S L OUT
10 W 9 C
4 12
GUARD
L P PRESS.
XDUCER.
VALVE
IN
WASTE
WASTE
18
OUT OUT
3
PUMP PUMP
HEAD HEAD
IN IN
WASTE
IN
2 OUT
ELUENT
VALVE
EGC VENT
EGC
Send To
Waste
NOTE: The EGC Degas fittings
cannot be serviced by the user.
INLET OUTLET
EGC EGC
IN OUT
Connected to
Pump Pulse TO PUMP/
DAMPER
Dampler
TO DEGAS
ELUENT IN EGC
DEGAS
TO DEGAS
ELUENT OUT
DEGAS
ELUENT OUT
CR-TC
Eluent
Out
Regen In
TO CR-TCEluent Out TO CR-TC
ELUENT OUT ELUENT IN
(yellow) WASTE,
Connected to (red)
GAS
TO INJ -TC Regen Out Eluent In
Inject Valve CR SEPARATOR
VALVE IN - P TO EN IN
Port P (2) E G g e)
R an
(or
TO CR-TC
REGEN OUT
Connected to (blue)
Connect to
TO SRS/AES Gas Separator
Suppressor REGEN OUT
Regen Out Assembly
10-32 fittings (P/N 043275) and ferrules (P/N 043276) are used for most
tubing connections. For tightening requirements, refer to Installation of
Dionex Liquid Line Fittings (Document No. 031432). The manual is on the
Dionex Reference Library CD-ROM (P/N 053891).
1/8-in fittings (P/N 052267) and ferrules (P/N 048949) are used for
connections to the suppressor REGEN OUT port and the eluent reservoir.
1. Turn off the pump from the ICS-2000 Control Panel in Chromeleon or from
the touch screen HOME page.
2. Open the ICS-2000 front door.
3. Disconnect each liquid line connected to the injection valve.
4. Follow the instructions provided in the Rebuild Kit to replace the rotor seal,
isolation seal, and stator face.
5. Reconnect all liquid lines to the injection valve (see Figure 5-9).
6. Close the door.
7. Turn on the pump.
Outlet Check
Valve
Primary
Pump Head
Secondary
Pump Head
Acorn Nuts
Inlet Check
Valve
NOTE The inlet check valve assembly housing has a 1/4-28 port.
The outlet check valve assembly housing has a 10-32 port.
7. Install the inlet check valve assembly (P/N 045722) on the bottom of the
primary pump head. Install the outlet check valve assembly (P/N 045721) on
the top of the head. Tighten the check valves fingertight, and then use a
wrench to tighten an additional one-quarter to one-half turn.
Overtightening may damage the pump head and check valve housing
and crush the check valve seats.
Preparation
1. Rinse the pump flow path with deionized water. Direct the flow to waste by
opening the waste valve on the secondary pump head (see Figure 5-13). To
open the valve, turn the knob one-quarter to one-half turn counterclockwise.
2. After rinsing, close the waste valve.
3. Close the eluent valve from the ICS-2000 Control Panel in Chromeleon (or
open the touch screen PUMP page and select Eluent Valve Closed).
4. To prevent contamination of pump parts, put on a pair of rubber gloves before
disassembling the pump head.
5. Place the head (front end down) on a clean work surface and lift off the spacer
to expose the piston seal (see Figure 5-11 or Figure 5-12).
O-Ring
(P/N 055752)
Piston Seal
(P/N 055870)
O-Ring
(P/N 055752)
Piston Seal
(P/N 055870)
Piston
(P/N 052840)
6. The piston does not come off as part of the pump head assembly because it is
captured by a magnetic retention system. After removing the pump head, hold
the shaft of the piston (near the base), tilt the piston slightly, and pull the
piston away from the pump.
c. Pull the seal off the end of the piston shaft and remove the piston from the
guide.
3. Hold the new piston rinse seal (P/N 048722) with the grooved side facing up.
4. Using your fingertip, gently press the piston rinse seal into the guide until the
edge of the seal is below the surface of the guide.
The piston rinse seal is made of soft plastic. Do not press on the seal
with anything hard or sharp, including your fingernail. If the seal is
nicked or gouged, it will not seal properly and may result in leaks.
5. Place the new O-ring (P/N 059283) into the groove in the guide.
6. Remove the O-ring from the groove in the flat side of the spacer and replace it
with the new O-ring (P/N 014895).
7. In one hand, hold the guide with the O-ring and piston rinse seal facing up
(this prevents the O-ring from falling out). In the other hand, hold the spacer
with the cavity facing down.
8. Gently press the guide into the cavity in the spacer until it is fully seated.
NOTE If the piston seal is not removed, make sure the 10-32
fitting plug(s) are tight and add more water. Make sure
the head contains no air bubbles, and then repeat Steps
2 and 5.
3. To seat the seal, push down on the spacer until it is flush with the head. A
clicking sound indicates that the seal is correctly seated.
4. Close the priming valve knob or waste valve knob.
Priming Valve
Waste Valve
4. If the O-ring is removed with the valve knob in Step 2, pull the O-ring off the
end of the knob (see Figure 5-14). If the O-ring is not removed with the valve
knob, insert a thin object (for example, the bent end of a paper clip) into the
cavity in the pump head and carefully pull out the O-ring. Do not scratch the
cavity.
Scratches in the cavity will cause leaks around the base of the knob
while the pump is being primed.
Valve Knob
O-Ring
(P/N 055752)
5. Slide a new O-ring (P/N 055752) over the end of the valve.
6. To reinstall the valve containing the new O-ring, turn the knob clockwise and
then tighten fingertight.
Unscrew
Disconnect
Unscrew
View A View B
Figure 5-15. Removing the Cell Front Cover
5. Slide the new cell assembly into the opening in the component panel until it
stops and the cell cover is flush against the component panel. The cells
electronic connector automatically plugs into a connector inside the ICS-2000
when you push the assembly into the opening.
6. Tighten the screws on the cell front cover fingertight.
7. Reconnect the inlet and outlet lines.
8. Turn on the ICS-2000 power.
9. Calibrate the new cell in Chromeleon or on the ICS-2000 touch screen
DIAGNOSTIC page. Cell calibration of a new cell consists of three parts:
Run the offset calibration, which measures the output of the cell
electronics alone and determines an offset value to be applied to raw
conductivity readings.
Run the slope calibration, which measures the output of the cell
electronics when a built-in dummy cell, which provides a constant
conductivity reading of 21 S, is automatically enabled. The slope value
is applied to raw conductivity readings.
Enter the cell constant measured at the factory. The ICS-2000 uses the cell
constant to determine the measured conductivity.
To calibrate the cell in Chromeleon:
a. Open the Wellness Panel (see Section 5.1.1)
b. Under Electric Conductivity Cell Calibration, click Offset Cal.
c. When the offset calibration is complete, click Slope Cal.
d. Click Calibration Details. Under Conductivity Cell Cal Details, enter
the Cell Constant value printed on the front of the cell.
e. In the field next to the Download button, verify that Current is selected
and then click Download to download the new value to the ICS-2000.
f. To ensure the new values are recorded in the ICS-2000 memory, do not
turn off the ICS-2000 power for at least 1 minute after downloading.
8. Connect the cable from the new suppressor to the ICS-2000 cable (align the
pins on the two connectors and push them together).
9. Orient the suppressor with the REGEN OUT fitting on top and the cables to the
right.
10. Push the cables into the opening in the component panel.
11. Align the top of the suppressor
with the guide line printed on the
component panel (see
Figure 5-19) and align the slots 1 Suppressor
Mounting Tabs
on the rear of the suppressor
with the tabs on the panel. 4 2 Suppressor Cable
Connector
12. Press the suppressor onto the
tabs and then slide it down until 3 Guideline for AES
3
it locks into place. Pull slightly
on the center of the suppressor to 1 4 Guideline for SRS
verify that it is securely fastened.
Note: Some suppressors require 2
more force to secure them onto 1
the tabs.
13. Connect the two eluent and two 1
regenerant lines to the new
suppressor.
14. Close the ICS-2000 front door.
7. Disconnect the heat exchanger outlet line from the guard column inlet.
8. Use an Allen wrench to remove the three screws from the heat exchanger
metal plate and remove the plate (with the tubing) from the heater.
9. Remove the plastic backing from the new heat exchanger (P/N 059979, with
0.25-mm (0.010-in) ID tubing; P/N 060943, with 0.125-mm (0.005-in) ID
tubing) and press the heat exchanger onto the heater. Replace the screws
removed in Step 8.
10. Connect the new heat exchanger inlet line to Port C on the injection valve and
connect the outlet line to the guard column inlet.
11. Reinstall the top metal plate and the heater cover.
Remove
11. Connect the cable from the new valve to the ICS-2000 cable. Feed the cables
back inside the ICS-2000.
12. Align the new valve on the component panel with the liquid ports facing up
and attach it with the two screws removed in Step 5. Reconnect the liquid
lines.
13. Turn on the power to the ICS-2000.
14. Prime the pump (see Section B.12).
Loosen
Leak
Sensor
7. Connect the cable from the new leak sensor to the ICS-2000 cable.
8. Feed the cables back inside the ICS-2000. Align the leak sensor with the
component panel opening and fingertighten the screw.
9. Make sure the leak sensor does not touch the bottom of the drip tray.
1. Connect a 10-mL syringe (P/N 054578) filled with isopropyl alcohol (IPA) to
the port in the primary pump head (see Figure B-23).
2. Open the waste valve on the secondary pump head (see Figure B-23) by
turning the knob one-quarter to one-half turn counterclockwise.
3. Click Pump On on the Chromeleon Control Panel to turn on the pump motor.
4. Open the priming valve on the primary pump head by turning it one-quarter to
one-half turn counterclockwise.
5. Use the syringe to slowly push alcohol through the pump.
6. Close the priming valve. Do not overtighten. Disconnect the syringe from
the priming valve.
7. Let the pump run for several minutes, to purge alcohol from the pump heads,
and then click Prime and flush the heads with the desired eluent.
Isopropyl alcohol may damage some columns. Be sure to thoroughly
rinse the alcohol from the pump, using the process described in
Step 7.
HIGH VOLTAGEDisconnect the main power cord from its source and
also from the rear panel of the ICS-2000.
2. The fuse holder is part of the main power receptacle (see Figure 5-28) on the
ICS-2000 rear panel. To remove the fuse holder, squeeze the tab on the top of
the holder to release it and pull the holder straight out of its compartment.
3. Replace the two fuses in the holder with new IEC 127 fast-blow fuses rated
3.15 amps (P/N 954745). Dionex recommends always replacing both fuses.
4. Reinsert the fuse holder into its compartment and push in until the tab clicks
in place.
5. Reconnect the main power cord and turn on the power.
Der EGC ist mit KOH, einer tzenden Lauge, oder MSA, einer tzenden
Sure befllt. Tragen Sie daher eine Schutzbrille und Handschuhe.
5. Install the luer cap (P/N 053981), provided with the EGC, onto the luer
adapter. This prevents leaks from the vent opening when you turn over the
EGC (see Step 7).
6. Lift the EluGen cartridge straight up and out of the cartridge holder.
7. Turn the cartridge upside down and set it in the EGC service position on top
of the ICS-2000 (see Figure 5-30 and Figure 5-31).
EGC Service
Position
Fitting Plugs
Inlet
Outlet
13. Connect EGC IN to the EGC INLET port (see Figure 5-34).
INLET Port
EGC IN
14. Locate the yellow 0.5 mL/min, 7 MPa (1000 psi) pressure restrictor coil
(P/N 053765) in the ICS-2000 Ship Kit (P/N 057905). Connect one end of this
coil to the EGC OUTLET port (see Figure 14). Leave the other end of the coil
unconnected.
Pressure
Restrictor
Coil
OUTLET
Port
15. Turn the EGC cartridge over, with the fittings facing down.Shake the EGC
vigorously and tap it with the palm of your hand 10 to 15 times. Check
that all trapped bubbles are dislodged.
Der EGC ist mit KOH, einer tzenden Lauge, oder MSA, einer tzenden
Sure befllt. Tragen Sie daher eine Schutzbrille und Handschuhe.
18. Remove the plug from the EGC vent opening and connect the EGC VENT line.
To avoid damaging the suppressor and CR-TC, always turn off the
suppressor and CR-TC before conditioning the EGC. The pump flow is
on during conditioning, however, no flow reaches the suppressor or
CR-TC.
12. Turn over the EGC (fittings facing down). Check for bubbles. If necessary,
shake and tap the EGC to remove bubbles.
13. Reinstall the EGC in the holder.
14. Reconnect the EGC cable.
15. Reconnect the CR-TC cable.
16. Record the serial number of the new cartridge in Chromeleon:
1234567890
NOTE You can also enter the cartridge serial number from the
Chromeleon Control Panel by clicking the EG Settings
button.
17. If you disabled the Automatically turn on with pump option in Step 3, re-enable
the option.
13. Reconnect the EGC IN and EGC OUT lines to the EGC inlet and outlet
ports.
14. Turn over the EGC (fittings facing down). Check for bubbles; if
necessary, shake and tap the EGC to remove bubbles.
15. Reinstall the EGC in the holder.
16. Remove the cap on the luer adapter by twisting. Save the cap.
17. Twist the luer lock and EGC VENT line into the luer adapter.
18. Do not connect the EGC cable until after hydrating the CR-TC.
4. Turn on the pump and set the flow to the rate recommended for your
application. Pump deionized water through the CR-TC for at least
10 minutes.
5. Turn off the pump and disconnect the coupler.
6. After hydrating the CR-TC,
Reconnect the line labeled TO INJ VALVE IN - P to port 2 on the
injection valve.
Reconnect the line labeled TO SRS/AES REGEN OUT to the
REGEN OUT port on the suppressor.
Der EGC ist mit KOH, einer tzenden Lauge, oder MSA, einer tzenden
Sure befllt. Tragen Sie daher eine Schutzbrille und Handschuhe.
6. Install the luer cap (P/N 053981), provided with the EGC, onto the luer
adapter. This prevents leaks from the vent opening when you turn over the
EGC (see Step 7).
7. Lift the EluGen cartridge straight up and out of the EGC holder.
8. Turn the cartridge upside down and set it in the EGC service position on top
of the ICS-2000 (see Figure 5-30 and Figure 5-31).
9. Disconnect the EGC IN and EGC OUT lines from the INLET and OUTLET ports
on the cartridge.
10. Lift the holder out of the top
cover and pull the liquid lines
through the tubing chase (see
Figure 5-44) to remove them
from the ICS-2000.
Tubing Chase
20. Connect the WASTE-GAS SEPARATOR to the gas separator waste tube.
21. Remove the union from the
EGC OUT and EGC IN lines EGC IN
EGC OUT
inside the holder and connect INLET
the lines to the OUTLET and Port
OUTLET
INLET ports on the cartridge. Port
22. Turn over the EGC (fittings facing down). Check for any bubbles, and if
necessary, shake and tap the EGC to remove them.
23. Reinstall the EGC in the holder.
24. Reconnect the EGC and CR-TC cables.
25. Remove the cap from the luer adapter on the EGC vent opening and re-
connect the EGC VENT line.
A.1 Electrical
Main Power 85 to 265 Vac, 47 to 63 Hz. The ICS-2000 power supply is auto-
sensing and requires no voltage adjustment
Fuses Two 3.15 amp fast-blow IEC 127 fuses (P/N 954745)
A.2 Physical
A.3 Environmental
Operating 35 MPa (5000 psi) maximum liquid path (tubing, valve, columns,
Pressure etc.)
LCD Touch VGA (320 x 240 pixels) liquid crystal display with touch screen,
Screen which provides:
Local control of ICS-2000 components: pump, detector,
suppressor, injection valve, eluent generator, CR-TC, column
heater, vacuum degas, TTLs, and relays
Display of conductivity data plot and operating status
Local control of calibration and diagnostic functions
A.5 Pump
Flow Rate Settable range: 0.00 to 5.00 mL/min (without eluent generator)
0.10 to 3.00 mL/min (with eluent generator), in 0.01 increments
Optimal Operational Range: 0.4 to 2.0 mL/min
Flow Accuracy <1% of set value within the optimal operational range
High Pressure 0.7 to 34.4 MPa (100 to 5000 psi) (without eluent generator)
Limit 2.1 to 20.6 MPa (300 to 3000 psi) (with eluent generator)
Low Pressure 0.0 to 33.7 MPa (0 to 4900 psi) (without eluent generator)
Limit 1.4 to 19.9 MPa (200 to 2900 psi) (with eluent generator)
Pressure Ripple <1% at 13.8 MPa (2000 psi) and 1.0 mL/min
A.6 Detector
This chapter provides instructions for the initial installation of the ICS-2000 Ion
Chromatography System.
EGC Holder
(upside down)
Eluent
Bottle
4. Remove the next layer of foam and remove the Ship Kit box (see Figure B-3).
Ship
Kit
Cutouts
for lifting
5. The bottom foam layer has two cutouts on either side of the ICS-2000
module; use these as hand holds when lifting the module (see Figure B-3).
6. Lift the ICS-2000 out of the bottom layer of foam and set it on a workbench.
Inspect the ICS-2000 for any shipping damage.
Two or more persons should lift the ICS-2000, which weighs 30 kg
(66 lb). Lift the ICS-2000 only from each side of the cabinet bottom.
Lifting from the front door will damage the door hinges.
Der ICS-2000 wiegt ber 20 kg. Daher sollte das Gert nur von zwei
oder mehr Personen angehoben werden. Greifen Sie dazu an beiden
Seiten unter das Gert. Heben Sie den Autosampler nicht an der
Vordertr an, da dadurch die Trangeln beschdigt werden knnten.
1. Remove the central processing unit (CPU), keyboard, mouse, and all
documentation from the computer box and place these items on a
workbench.
2. Remove the monitor from its box and place it on the workbench.
3. Follow the instructions in the computer installation guide to hook up
the monitor, mouse, keyboard, and CPU.
4. Go on to Section B.4, Installing the Chromeleon Software License..
IMPORTANT
Installing DX-LAN Devices
If you will also be installing a module that requires a DX-LAN interface
connection, verify that the following steps are completed before continuing:
1. If it is not already installed, install the DX-LAN card in the module. Refer
to the operators manual for the module for installation instructions.
2. Install the DX-LAN card in the computer.
3. Connect the module to the DX-LAN.
4. Install the DX-LAN card driver.
Instructions for Steps 2 through 4 are in Installing the Chromeleon IC System
(Document No. 031883).
1. Plug the dongle into the appropriate port on the computer (either the parallel
or USB port, depending on the type of dongle). Tighten the connector screws
on a parallel port dongle.
2. If it is not already running, start the Chromeleon Server Monitor (select
Start>Programs>Chromeleon>Server Monitor). The Server Monitor
window opens.
5. Select File>Preferences to open the Preferences dialog box (see Figure B-5).
Select the License tab and click Dongle. Enter the Key Code exactly as it
appears on the Chromeleon Software License key code sheet. Click OK.
6. Check the Server Monitor status. It should now display Chromeleon Server
is running idle. (see Figure B-6).
B
ICS-2000
Figure B-8. Example Connections: Two Dionex USB Modules Connected to the ICS-2000
The USB standard limits the USB cable length to 5 meters (16 ft). Each
USB device can be separated from the PC by no more than five hubs.
Thus, each USB device can be located no more than 30 meters
(32 yds) from the PC.
The power supply cord is used as the main disconnect device. Make
sure the socket-outlet is located near the ICS-2000 and is easily
accessible.
4. Go on to Section B.8.
12. The ICS-2000 System dialog box opens (see Figure B-13). The
General page shows the Device Name (Pump_ECD), the Mode
(Live), and the Module Serial No.
13. Close the dialog box and then save the configuration (select
File>Save Installation).
14. If you are going to add one or more DX-LAN devices to the timebase,
go on to Section B.8.2. Otherwise, exit the Server Configuration
program and then go on to Section B.9.
5. On the General tab page, clear the Demo Mode check box if it is
currently selected, and then select the DX-LAN Device ID number.
Note: Devices display their DX-LAN ID numbers when their power
is turned on.
6. Click OK to close the dialog box.
7. Save the configuration (select File>Save Installation).
8. Exit the Server Configuration program.
9. Go on to Section B.9
Before beginning:
Remove the guard column, separator column, and suppressor from their
boxes.
Remove the fitting plugs from the ends of each column and from all ports on
the suppressor.
Discard any tubing temporarily connecting the suppressor ports.
5. Go on to
Section B.9.2.
3. Orient the suppressor with the REGEN OUT fitting on top and the
cable to the right.
4. Feed the cable into the opening in the component panel.
5. Align the top of the suppressor with the guideline printed on the
component panel (see Figure B-19) and align the slots on the rear of
the suppressor with the tabs on the panel.
8. Remove the fitting plug from REGEN IN and connect it to the REGEN
IN port on the suppressor. Do not connect the REGEN OUT port until
after completing the eluent generator setup (Section B.13).
9. Replace the cover on the column heater, making sure the GUARD IN
line is aligned with the slot on the side of the heater cover.
10. Go on to Section B.10.
3. Place the gas separator waste tube assembly into the waste container.
Make sure the tubing junction (where the white Teflon tubing meets
the black polyethylene tubing) is above the top of the container and
that the gas separator waste tube and the waste container are open to
the atmosphere (see Figure B-22).
4. Go on to Section B.11.
Waste, Gas
Separator Line
Gas Separator
Waste Waste Tube Assembly
Container
5. Go on to Section B.12.
Secondary
Pump Head
Waste Valve
(close)
Port
Primary
Pump Head
6. Draw the syringe back to begin pulling eluent through the flow path.
It may take several syringe draws to remove all air or previous eluent
from the tubing. Draw out a minimum of 20 mL of eluent to ensure
that the vacuum degas assembly is primed.
7. After priming the lines thoroughly, close the priming valve. Do not
overtighten the priming valve.
8. Touch Eluent Valve Closed on the touch screen PUMP page.
1. Check that the priming valve on the primary pump head is closed (see
Figure B-25).
Secondary
Pump Head
Waste Valve
(open)
Primary
Pump Head
Priming Valve
(close)
2. Open the waste valve on the secondary pump head by turning the
knob one-quarter to one-half turn counterclockwise (see
Figure B-25). Opening the valve directs the eluent flow path to waste
and eliminates backpressure.
3. Touch Prime on the ICS-2000 touch screen HOME page. Confirm that
the waste valve is open by pressing OK when the reminder message
appears. The pump will begin pumping at approximately 3.0 mL/min.
4. Continue priming the ICS-2000 until no air bubbles are exiting the
pump waste line.
5. Press Pump Off.
6. Close the waste valve. Do not overtighten. The pump is now ready
for operation.
NOTE If the two standard priming procedures described above
are unsuccessful, prime the pump with isopropyl
alcohol. See Section 5.15 for instructions.
EGC
Note: The CR-TC is
installed inside the
holder and is not
EGC Holder visible after installation
EGC and
CR-TC Cable
Connections
Figure B-27 illustrates the liquid flow path through the eluent generator
components after all plumbing connections are complete. The EGC and CR-TC
are shipped in separate boxes. The EGC degas assembly is installed inside the
EGC holder at the factory. When installation is complete, all components fit inside
the EGC holder.
EGC VENT
EGC
Send To
Waste
INLET OUTLET
EGC EGC
IN OUT
Connected to
Pump Pulse TO PUMP/
DAMPER
Dampler
TO DEGAS
ELUENT IN EGC
DEGAS
TO DEGAS
ELUENT OUT
DEGAS
ELUENT OUT
CR-TC
Eluent
Out
Regen In
TO CR-TCEluent Out TO CR-TC
ELUENT OUT ELUENT IN
(yellow) WASTE,
Connected to (red)
GAS
TO INJ -TC Regen Out Eluent In
Inject Valve CR SEPARATOR
VALVE IN - P TO EN IN
G
Port P (2) R E an g e)
(or
TO CR-TC
REGEN OUT
Connected to (blue)
Connect to
TO SRS/AES Gas Separator
Suppressor REGEN OUT
Regen Out Assembly
3. Remove the plugs from the four ports, labeled P on Figure B-29.
Do not remove the two electrical connections, labeled E on the
figure.
4. With the CR-TC oriented as shown in Figure B-30, connect the lines
to the corresponding ports on the CR-TC. Match the color coded
labels on the EGC holder lines to the color coded labels for the CR-
TC ports.
NOTE To help retain the fittings and ferrules on the lines,
remove the union from each line just before
connecting the line to a port.
Blue
Red Yellow
Orange
5. Check that none of the lines are caught under the CR-TC and then
push the CR-TC onto the metal stud inside the EGC holder (see
Figure B-31). Push down until the CR-TC snaps into place.
6. Feed the CR-TC cable through the slot on the side of the EGC holder
(see Figure B-32).
CR-TC
Cable
Fitting Plugs
INLET
OUTLET
2. Set the EGC in the service position on top of the ICS-2000 (see
Figure B-34 and Figure B-35).
EGC Service
Position
3. Orient the EGC with the cable toward the cartridge holder (see
Figure B-36).
EGC Cable
6. Turn the EGC cartridge over (with the fittings facing down). Shake
the EGC vigorously, and tap it with the palm of your hand 10 to
15 times. Check that all trapped bubbles are dislodged.
Der EGC ist mit KOH, einer tzenden Lauge, oder MSA, einer tzenden
Sure befllt. Tragen Sie daher eine Schutzbrille und Handschuhe.
Der EGC ist mit KOH, einer tzenden Lauge, oder MSA, einer tzenden
Sure befllt. Tragen Sie daher eine Schutzbrille und Handschuhe.
5. Direct the EGC vent line to the rear of the ICS-2000 and snap it onto
one of the tubing clips on the rear panel. Direct the vent line to the
waste container and insert it down into the gas separator waste tube
about 4 to 6 inches.
6. Set a temporary waste container, such as a beaker, on top of the
ICS-2000 and direct the yellow backpressure coil from the EGC
OUTLET port to the container.
To avoid damaging the suppressor and CR-TC, always turn off the
suppressor and CR-TC before conditioning the EGC. The pump flow is
on during conditioning, however, no flow reaches the suppressor or
CR-TC.
5. Turn on the pump and set the flow to the rate recommended for your
application. Pump deionized water through the CR-TC for at least 10
minutes.
6. Turn off the pump and disconnect the coupler.
7. Connect the TO SRS/AES REGEN OUT line to the REGEN OUT port on
the suppressor.
8. Reconnect the TO INJ VALVE IN - P line to port P on the injection
valve.
9. Reconnect the EGC cable.
1234567890
053762 4-mm Pressure Restrictor 2.0 mL/min 3.5 MPa (500 psi)
1.0 mL/min 1.75 MPa (250 psi)
053764 2-mm Pressure Restrictor 0.50 mL/min 3.5 MPa (500 psi)
0.25 mL/min 1.75 MPa (250 psi)
Table B-4. Pressure Restrictor Coils
3. In the Browser, select File>New and select Sequence (using Wizard) (see
Figure B-47).
NOTE For details about a Sequence Wizard step, click the Help
button on the Sequence Wizard page.
4. Click Next>.
The Sequence Wizard Timebase page appears (see Figure B-49).
NOTE You can edit the sequence later, to add more samples
and select the injection parameters appropriate for your
application.
12. Click Next> to omit this step and define standard injections later. Or, define
one or more standard injections, and then click Next>.
The Methods & Reporting page appears
15. To select a report template, click the Browse button, go to the Dionex
Templates/Reports folder, and select a template (for example, default_letter).
16. Click Next>.
The Saving the Sequence page appears.
17. Enter a name for the sequence and a location (see the example in
Figure B-54).
18. Click Next> and then click Done on the last page of the Sequence Wizard.
The Browser appears, displaying the sequence you created (see Figure B-55).
Notice the three files in the top pane of the Browser window.
The .pgm file (program) contains commands and parameters for controlling
the ICS-2000, controlling the autosampler (if used), and acquiring sample
data.
The .qnt file (quantification method) contains peak identification and area
determination parameters.
The .pan file (Control Panel) lets you control and/or monitor the ICS-2000
and other modules that are connected to the timebase.
19. Double-click the .pan file to open the Control Panel for your system (see the
example in Figure B-56).
NOTE If the Connected check box is not selected, click the box
to connect the ICS-2000 to the Control Panel.
Notes
issued from the AS50 front panel or from Chromeleon) will control the
ICS-2000 injection valve. For Chromeleon control, the AS50 must be
added to the ICS-2000 timebase (see Section B.8). For front panel
control, the AS50 and ICS-2000 can be set up for stand-alone operation.
This requires a TTL cable connection between the AS50 and the ICS-
2000. See Section D.3 for stand-alone setup instructions.
Route AS50
needle seal line
to ICS-2000
injection valve
NOTE For PEEK waste lines, sharply bend the waste line
tubing about 5-mm from the end to ensure it stays in
place in the opening in the drip tray. When bending the
tubing, be sure not to restrict the inner diameter.
7. If you have not already done so, add the AS50 to the Chromeleon timebase
(see Section B.8).
Help Prompt
Figure B-62. AS50 Time Function Out Screen
4. Locate the relay control cable (P/N 047946) supplied with the AS40.
5. Remove the 2-position connector plug from the pair of wires labeled LOAD on
the relay control cable.
6. Locate the 12-position connector
plug (P/N 923686) in the ICS-
2000 Ship Kit (P/N 057905) (see Position 1
8. Connect the relay control cable's 10-position connector plug to the RELAY
CONTROL connector on the AS40 rear panel (see Figure B-65).
9. Plug the 12-position connector plug into the TTL and relay connector on the
ICS-2000 rear panel (see Figure B-65). Make sure the red wire connects to
position 1 and the green wire connects to position 2.
The AS40 Load relay is now dedicated to the ICS-2000 RELAY OUT 1. Use this
relay for running with Chromeleon. When you set up the ICS-2000 application
(see Section B.14), commands for controlling the AS40 Load relay and switching
the ICS-2000 injection valve are included by default in the program file (see
Figure B-66).
Connect to
gas source
1. Pull out the air regulator knob and turn it fully counterclockwise to
ensure there is no pressure to the reservoir when the gas source is
turned on.
2. Turn on the gas source.
3. Pull out the air regulator knob and turn it clockwise to pressurize the
eluent reservoir.
4. Adjust the pressure to between 30 and 40 kPa (5 and 6 psi). Push the
regulator knob back in.
Setzen Sie den Eluentbehlter auf keinen Fall einem Druck ber 0,07
MPa aus.
Drain Tube
Drain Tube
Primary Pump
Head
Figure B-68. Primary and Secondary Pump Heads with Drain Tubes
b. Push the 152-cm (60-in) piece of clear tubing onto the drain tube on the
top of the primary pump head. This is the waste line. Route this waste line
through the lower tubing chase and out the back of the ICS-2000.
c. Push the 15-cm (6-in) piece of clear tubing over the drain tube on the top
the secondary pump head. Push the other end of the tubing over the drain
tube on the bottom of the primary pump head.
d. Place a 10 mL syringe (P/N 054578) at the end of the waste line, press the
syringe against the line to form a seal, and draw out all of the air.
e. Push approximately 5 cm (2 in) of black 0.25-mm (0.01-in) ID tubing
(P/N 057057) into the end of the waste line.
When the Control Panel opens, the controls on the panel are not enabled (see
Figure B-70).
3. To connect the Control Panel to the ICS-2000, open the Control menu and
click the name of the ICS-2000 timebase at the bottom of the menu.
The Control Panel is now connected to the ICS-2000 and the controls are
enabled (see Figure B-71).
Figure B-71. ICS-2000 Traditional System Control Panel (Connected to the ICS-2000)
The LCD touch screen on the ICS-2000 front panel allows local control of most
ICS-2000 functions. This chapter describes how to operate the ICS-2000 from the
touch screen.
When the ICS-2000 starts up, the touch screen displays a startup page. This is
followed by the INFORMATION page, which displays version and serial numbers
and a list of installed options. After a few seconds, the HOME page (see
Figure C-1) is displayed. The HOME page provides controls for all normal, daily
operation of the ICS-2000.
Status Field
Edit Field
Command
Button
(selected)
Command
Button
(unselected)
NOTE To adjust the screen contrast, open the front door and
adjust the knurled knob under the screen (see
Figure 2-8).
Edit fields and command buttons have blue text on a lightly shaded
background. Fields that display status information are not shaded.
Edit fields have square corners and command buttons are rounded.
To edit a field or select a command button, touch and release the field or
button with your fingertip.
NOTE When you touch a button or edit field, the action takes
effect when you lift your finger. If you unintentionally
touch a button or field, slide your finger away from the
button or field before lifting, to cancel the action.
Touch CANCEL
to cancel the
entry and return
Touch a to the previous
number to page.
enter the
value. Touch ENTER to
confirm the
entry and return
to the previous
page.
Touch a page
name to display
the page.
Touching a page name on the menu of pages displays the selected page. For
example, touching SUPPRESSOR displays the SUPPRESSOR page (see
Figure C-4).
Touch to
select a
different
option.
Touch to
return to the
Home page.
Touching opens a list of options from which to choose (see Figure C-5).
Touch an
option to
select it.
Note: To cancel
and retain the
current selection,
touch outside of
the list.
HOME
STATUS
PUMP
Calibration Pages Diagnostic Pages
COLUMN
INFORMATION HEATER
INPUT/OUTPUT DEGAS
DIAGNOSTIC
Status Field
Edit Field
Command
Button
(selected)
Command
Button
(unselected)
Pressure Display
The HOME page indicates the current pressure reading from
the pressure transducer. The pressure unit can be set to psi
(the factory default), MPa, or bar (see Section C.7.1).
The minimum and maximum pressure limits are displayed
below the current pressure reading. If the pressure reading
goes outside these limits, the pump is stopped and an error
message appears in the Chromeleon Audit Trail and on the
touch screen. See Section 4.1 for troubleshooting
information. See Section C.7.1 for how to set the pressure
limits.
Flow Rate
The flow rate can be set to 0.00 (which turns off the pump) and to between 0.05
and 5.0 mL/min. The recommended operating flow rate is between 0.4 and
2.0 mL/min. Refer to the column manual for the flow rate to use with your column
and application. Column manuals are included on the Dionex Reference Library
CD-ROM (P/N 053891), located in the ICS-2000 Ship Kit (P/N 057905).
Prime
The Prime button is reserved for use during the pump head priming procedure.
Touching the Prime button displays a confirmation page that reminds you to open
the waste valve. Open the valve, and then touch the OK button to begin priming.
Or, to exit the page without priming, touch the Cancel button. To stop priming,
touch the Off button. During priming, the flow rate is approximately 3.0 mL/min.
See Section B.14 for detailed priming instructions.
Eluent Level
Use this field to monitor the amount of liquid in the eluent reservoir. When you
fill the reservoir, enter the amount of liquid in the reservoir. For example, if the
reservoir contains 200 mL and you add 1 liter, enter 1.2 liters. Thereafter, the ICS-
2000 determines the eluent usage by monitoring the flow rate and the length of
time the pump is on. The ICS-2000 updates the Eluent Level field as the eluent is
used up. A warning first appears if the level falls below 200 mL. Warnings are
repeated at 100 mL and 0 mL.
Conductivity Readings
The offset conductivity is displayed in large numbers above
the total conductivity. The offset conductivity is the total
conductivity minus the current offset.
To determine the offset, allow the system to equilibrate after
startup. At equilibration, the conductivity reading is the background conductivity
(the eluent conductivity before sample injection). Touch Autozero to zero the
conductivity and offset this background reading from the total reading.
Suppressor Controls
The Suppressor controls indicate the suppressor current
setting and the type of suppressor installed.
To set the suppressor type, change the current setting, or
turn the suppressor on or off, touch the suppressor button.
The SUPPRESSOR page appears (see Section C.9).
Plot Controls
The plot offset button offsets the current background conductivity reading
and sets the plot baseline to zero. Subsequent output is plotted in relation to
the new zero point. Offsetting the plot does not affect the data sent to
Chromeleon, the analog output, or the data displayed on other pages.
The range field sets the highest conductivity reading displayed on the plot
(the vertical scale). If the tops of peaks are cut off, select a higher range. If
peaks are short, select a lower range.
The elapsed time field displays the time since injection.
The plot time field sets the length of time plotted on the page (the horizontal
scale).
The eluent bottle depicts the level of liquid in the eluent reservoir. The scale is
always 0 to 4 liters (regardless of the size of reservoir installed). After filling
the reservoir, go to the HOME page and enter the amount of liquid in the
reservoir (see Section C.4.1). The ICS-2000 monitors the eluent usage, based
on the flow rate and the length of time the pump is on. The liquid level in
the bottle falls as the reservoir empties. An error message is first displayed on
the screen when the level falls below 200 mL. The message is repeated at
100 mL and then at 0 mL.
The fields to the right of the bottle display the status of four other operating
parameters. You can choose from eight possible parameters to display here.
Figure C-9 shows the default parameters: Eluent time, Flow rate, EGC conc,
and Suppressor. To select different parameters, touch the Setup button. See
Section C.6.1 for details about how to change the status parameters, and
Section C.6.2 for a description of each parameter.
Touching the Busy button displays the BUSY page (see Figure C-10).
2. Touch the down arrow next to the field you want to change. A list of
the available status parameters appears (see Figure C-12).
Select the
pressure unit
Select the
degas option
Duty Cycle: The degas pump cycles on and off according to the times
specified in the Duty Cycle and Time Off fields. Duty Cycle specifies
for how long the degas pump runs during a cycle; Time Off specifies
the time between cycles.
Monitor: The ICS-2000 monitors the degas pressure reading and
turns the degas pump on and off as required.
Touch here to
Touch here drop-down a
to enter a list of existing
new serial serial numbers
number
Section B.13.5), not on the ELUENT GENERATOR page. If you enter the
serial number first in Chromeleon, the number is displayed on the
ELUENT GENERATOR page. However, if you enter the serial number on
the ELUENT GENERATOR page, it will not be available in Chromeleon.
If you are running the ICS-2000 without ICS-2000 control, enter the
cartridge serial number by touching the Serial Number field on the
ELUENT GENERATOR page (see Figure C-16) and entering the serial
number on the keypad.
If you are reinstalling a cartridge that was used previously, touch the
down arrow instead of the number field. A list of the last five entered
serial numbers drops down. Choose the serial number of the cartridge
from the list.
NOTE The CR-TC power always turns off when the pump
is off, regardless of whether the Automatically turn
on with pump option is selected.
Suppressor Controls
The suppressor type is typically selected at initial installation and does not
need to be changed. However, if a different type of suppressor is installed
later (or if the suppressor is removed from the system), touch the Type field
and select the new suppressor type (None/MMS, SRS, or AES) from the list.
To set the current, touch the Current field and enter the desired setting (in
milliamperes) on the number pad.
The suppressor current setting to use depends on several variables: the
suppressor type, the column, the eluent, and the flow rate. Refer to the
suppressor manual for the recommended current setting for your application.
The suppressor manuals are included on the Dionex Reference Library CD-
ROM (P/N 053891), located in the ICS-2000 Ship Kit (P/N 057905).
To manually control the suppressor power, touch the Off and On buttons.
To automatically control the suppressor power simultaneously with the pump
flow, select Automatically turn on with pump.
NOTE The suppressor always turns off when the pump is off,
regardless of whether the Automatically turn on with
pump option is selected.
The Analog Out options on the DETECTOR page (see Figure C-19) let
you configure the output for your application.
Output: Select Normal to output a signal corresponding to the offset
conductivity reading from the detector (see Conductivity Readings
on page C-9) and the selected Analog Out parameters. Use the other
two settings to calibrate an analog-to-digital converter device. Select
Zero to set the output signal to zero volts. Select Full Scale to set the
output signal to the full-scale voltage (1000 mV).
% Offset: To adjust the zero position of the analog output when it is
plotted, enter a percentage in the % Offset field. The value entered is
a percentage of the full-scale analog output. An offset allows a
recording device to plot the signal if it becomes negative. The offset
percentage does not affect the magnitude of the output signal.
Polarity: Select the polarity of the analog output signal: positive (the
default settings) or negative. In applications in which the analyte
output is lower than the background conductance, the polarity must
be negative to have peaks instead of dips on the chromatogram.
Range: Select the range in microSiemens (S) of a full-scale detector
response. For the ICS-2000, the full-scale voltage is 1000 mV. For
example, if the range is 100 S, then 1 S is equal to 10 mV from the
analog output. The range to use depends on the conductivity readings
expected for the application.
shown in large bold type on the HOME and STATUS screens, with the other
value in smaller type.
Connector
Pin Function Position Description
1
1 Solid State Relay Contacts Output
RELAY 2
OUT 3
2 Solid State Relay Contacts Output
4
TTL OUT 1 5 TTL Output 1 (1 k pull up to +5, 100 mA sink)
(+) 2 6 TTL Output 2 (1 k pull up to +5, 100 mA sink)
1 7 TTL Input 1 Inject/Load
TTL IN 2 8 TTL Input 2 Autozero
(+) 3 9 TTL Input 3 Pump/Suppressor On
4 10 TTL Input 4 Mark
TTL GND 1 11 Ground Note: The TTL input
functions can be reassigned
(-) 2 12 Ground to different inputs.
b. Plug the connector into the 12-pin connector on the ICS-2000 rear panel.
c. Connect the wires from the ICS-2000 connector plug to the TTL or relay
connector pins on the other module(s). Additional connector plugs are
provided with other Dionex modules.
3. If you connected a TTL input, verify that the correct function is assigned to
the input and that the correct input control type is selected. Select different
settings if necessary. Input functions and control types are assigned from
either the INPUT/OUTPUT touch screen page or Chromeleon (see
Section D.1.1).
To select TTL input functions and the control type from the touch screen,
go to the INPUT/OUTPUT touch screen page (see Figure D-4).
Select a
function
Select a
Control Type
The action of the positive (rising) edge depends on the function: For
the Load/Inject function, the rising edge switches the injection valve
to the Inject position. For the Pump On function, the rising edge turns
off the pump (and suppressor). For Autozero and Mark, the rising edge
has no effect.
Inverted Edge: The inverted
edge mode works identically to
the normal edge mode except
that the positive and negative
edges are reversed in function.
The TTL outputs are normally at 5 volts. Setting a TTL output to 0 volts turns on
the action in the connected device.
The TTL and relay output states can be controlled from Chromeleon, either by
issuing direct control commands from the Chromeleon control panel or by
including the commands in a control program. Figure D-5 shows an example relay
program command for controlling an AS40 autosampler.
To control the TTL and relay output states from the touch screen, select
INPUT/OUTPUT from the menu of pages. The INPUT/OUTPUT page appears (see
Figure D-4).
To set a relay, touch the relay field and select the desired state (Open or
Closed). For example, if Relay 1 is connected to the Load input on an AS40
autosampler, touch the Relay 1 field and select Closed to start the AS40 load
cycle.
To set a TTL outputs, touch the TTL field and select the desired state (5 Volts
or 0 Volts).
To attach a wire to a connector plug, strip the ends of the wire, insert into the
desired connector position, and tighten the locking screw with a screwdriver.
When attaching wires to the connector plug, be careful not to allow
stray strands of wire to short to the adjoining position on the
connector.
3. Plug the connector plugs into their respective TTL/Relay connectors on the
rear panel of each module.
Make sure the red wires connect to active (+) pins on each module and the
black wires connect to ground (-) pins.
+ ANALOG
- OUTPUT
TTL 2 OUT (+) TTL 1 IN (+)
TTL 1 OUT (+) TTL 2 IN (+)
1
1 TTL 3 IN (+)
RLY 2 OUT
RELAY 2 TTL 4 IN (+)
OUT RLY 1 OUT TTL
3
2 IN/OUT (-)
4
TTL OUT 1 5 1 2 3 4 5 6 7 8 9 10 11 12
red pos. black pos.
(+) 2 6 1,3,5,6 4,11,12,12
1 7 red: pos. 7,8,9
TTL IN 2 8
(+) 3 9 Inject
Pump On/Off
4 10 Autozero
TTL GND 1 11
(-) 2 12 black: Start/Continue Schedule
pos. 11,12,12
Start Acquisition
Help Prompt
Figure D-9. AS50 Time Function Out Screen
Help Prompt
Figure D-11. Example AS50 Method for Stand-Alone Operation
Pump
057937 Primary pump head assembly
057938 Secondary pump head assembly
045721 Outlet check valve assembly, 10-32
045722 Inlet check valve assembly, 1/4-28
045994 Check valve cartridges
052840 Piston
055870 Piston seal
048722 Piston rinse seal
055752 O-ring for waste valve or priming valve
057945 Eluent valve
054578 10-cc syringe (for priming eluent lines)
Eluent Generator
058900 EluGen EGC II KOH cartridge (for anion separations)
058902 EluGen EGC II MSA cartridge (for cation separations)
058069 EGC holder and degas assembly
060080 CR-ATC (for anion separations)
060411 CR-CTC cation (for cation separations)
Relay/TTL
923686 12-position connector plug
043598 Twisted pair of wires
Miscellaneous
954745 IEC 127 fast-blow fuses, rated 3.15 amps
060494 USB cable, 5 m (16 ft)
060392 External USB hub
058053 Leak detector
Maintenance Kits
055647 AS40/ASM preventive maintenance kit
060581 AS50 preventive maintenance kit
057954 ICS-2000 preventive maintenance kit
Analytical Column
Synonymous with Separator Column.
Band Spreading
The broadening of the sample band as it travels through the column. Band
spreading can also occur in the injection valve, detector cell, and interconnecting
tubing.
Calibration Curve
A graph showing detector response in peak height or area versus analyte
concentration.
Capacity Factor (k)
The number of column volumes of eluent, pumped through the column, required
to elute an analyte. Capacity factor is a dimensionless measure of retention which
is independent of column length or eluent flow rate. It is calculated as follows:
tr to
k = -------------
-
to
k = G
The value of k depends upon the surface area of, and distance between, the
electrode faces in the conductivity detector cell.
k = lA
Where: l = length
A = area of one electrode (the other electrode is equal to
the first)
Channeling
The preferential flow of liquid along more open, less resistant paths through the
column packing. This causes Band Spreading.
Column Efficiency (N)
A measure of the narrowness of analyte bands as they elute from the column.
High efficiency is desirable because resolution between closely spaced bands
improves with greater efficiency. For a symmetrical (Gaussian) peak, column
efficiency can be determined by the following:
N = 5.54(t1/W1/2)2
Where: t1 = the peak retention time (in seconds)
W1/2 = the peak width at 1/2 height (in seconds)
Column efficiency is proportional to column length: for a given resin and column
diameter, increasing the column length increases the column efficiency.
Synonymous with Theoretical Plates.
Column Selectivity (a)
Describes the relative separation of the band maxima between two adjacent peaks.
Selectivity can be determined by the following:
a = (t2 - t0)/(t1 -t0)
Where: t1 and t2 = retention time of components 1 and 2, respectively
t0 = retention time of unretained components (void volume)
Concentrator Column
A short column used to retain and concentrate analytes from a measured volume
Packing
The material that fills a chromatographic column; usually a resin or silica-based
material.
Pellicular Resin
A resin with a solid, nonporous core coated with a thin layer of more porous
material. The exchange sites of pellicular ion exchange resins are located only on
the surface layer of the bead. These resins have a low ion-exchange capacity.
Pre-Column
Synonymous with Guard Column.
Regenerant
A dilute acid or base that converts ion exchange sites in a MicroMembrane
Suppressor back to the form that suppresses the eluent conductivity.
Resin
See Ion-Exchange Resin.
Resolution (R)
A measure of the separation between two sample components. This is expressed
as the ratio of the distance between the two peak maxima to the mean value of the
peak width at the baseline.
R = 2(t2 - t1)/(W2 + W1)
Where: t1 and t2 =the retention times of components 1 and 2,
respectively
W1 and W1 = the baseline width of peaks 1 and 2,
respectively (measured in the same units as the retention
time)
R is proportional to the square root of efficiency (N). A value of R = 1.5
represents baseline separation of the two peaks.
Retention Time
The time from injection to peak maximum; the basis for identification of a species
in chromatographic analysis.
Separator Column
The column used to perform a chromatographic separation; also called an
analytical column.
Siemens (S)
Unit measure of conductance; the reciprocal of the electrical resistance of a
solution.
Suppressor
A device used to minimize eluent conductivity and convert sample species to a
common form, thus increasing detection sensitivity.
Temperature Coefficient
The percent of change in the conductivity of a solution with a 1 oC change in
temperature. Every solution has a characteristic temperature coefficient which is
determined experimentally.
Theoretical Plates (N)
See Column Efficiency.
Void Volume (V0)
The volume occupied by the eluent in a packed column. This volume includes the
volume between the injection valve and the column, as well as between the
column and the detector cell. Unretained components are eluted in the void
volume.
D
Daily maintenance, 3-23
Data
Backing up, F-2
E Installation, B-29
Setting the concentration, C-21
EGC, 2-6, 2-27 Specifications, A-4
Concentration upper limits, 4-6, C-21 Eluent Generator page, C-19
EGC-1 connector, 2-6, 5-43, B-37 Eluent level, 3-7, B-53, C-7
Entering the serial number, C-20 Eluent reservoir
Expiration date, C-21 Connecting, 3-9, B-25
Home page controls, C-7 Filling, 3-7
Installation, B-29 Gas connection, B-63
Life remaining percentage, C-21 Monitoring liquid level in, 3-7, B-53, C-7
Service area, 2-6 Pressurization, B-64
Vent line installation, B-38 Storage on top cover, 2-6
EGC alarms Eluent valve, 2-8, B-26, C-19
EGC board not present, 4-5 Replacing, 5-33
EGC-1 calibration error, 4-5 EluGen cartridge
EGC-1 disconnected error, 4-6 See EGC
EGC-1 invalid concentration vs flow rate End-line filter
error, 4-6 See Filter, end-line
EGC-1 invalid flow rate error, 4-6 Environmental specifications
EGC-1 over-current, 4-7 Humidity, A-1
EGC-1 over-voltage, 4-7 Operating pressure, A-1
EGC Resistive Load, 5-4 Operating temperature, A-1
EGC Verification, 5-4 Equilibration time, 3-13
EGC-1 connector, 2-6, 5-43, B-37 Error messages, 4-3 4-12
EGC-2 connector (reserved for future use), 2-6 Column heater exceeds safe temperature,
Elapsed time, C-9, C-11 4-3
Electrical specifications Column heater open circuit, 4-3
Fuses, A-1 Column heater short circuit, 4-3
Main power, A-1 CR-TC over current error, 4-4
Eluent CR-TC stopped for zero flow rate, 4-4
Aqueous, 3-24, 4-17 Degas calibration failed, 4-5
Concentration, C-21 EGC board not present, 4-5
Degassing, 2-22 EGC-1 calibration error, 4-5
Delivery process, 1-2 EGC-1 disconnected error, 4-6
Filtering, 3-7 EGC-1 invalid concentration vs flow rate
See also Eluent generator error, 4-6
Eluent bottle EGC-1 invalid flow rate error, 4-6
See Eluent reservoir EGC-1 over-current, 4-7
Eluent generator, 2-6, 2-27 EGC-1 over-voltage, 4-7
Cartridge holder, 2-27, B-31 Hardware not present, 4-7
Cartridge replacement procedure, 5-38 Leak sensor wet, 4-7
Controlling automatically, C-21 Load/inject valve error, 4-9
Controlling manually, C-21 Option not installed, 4-9
Degas tubing assembly, 2-13, 2-27 2-28 Pressure slope calibration error, 4-9
PEEK Pressurizing
Conductivity cell body, 2-32 Eluent reservoir, B-64
Physical specifications Prime button, C-7
Decibel level, A-1 Priming, 3-10, 5-36, B-25 B-28
Dimensions, A-1 Pump is out of prime, 4-16
Weight, A-1 With a syringe, B-26
Piston seals With isopropyl alcohol, 5-36
Replacement procedure, 5-18 5-19, With the Prime button, B-28
5-21 5-22 Priming valve
Pistons Opening, 2-25
Replacement procedure, 5-22 O-ring replacement procedure, 5-23
Plot offset, C-11 Printing, F-1
Plot page, C-10 C-11 Processing samples, 3-2, 3-20 3-21
Elapsed time, C-11 Automatically (batch), 3-2, 3-21
Plot offset, C-11 Manually, 3-2, 3-20
Range, C-11 Product warranty, 5-1
Plotting detector output, 2-21, C-11 Programs (Chromeleon), 2-14, 2-18, 3-18
Plumbing 3-21
Columns and suppressor, B-25 Run time, 2-18
Injection valve, 5-15 PSI, C-17
Replacing tubing and fittings, 5-14 Pulse damper, 2-13, 2-26
Troubleshooting, 5-11 Pump, 2-24
Polarity Flow rate, 2-8, C-6
Selecting the conductivity polarity, C-25 Home page controls, C-6
Potassium hydroxide Leaks, 4-14
EluGen cartridge, 2-27 Operating pressure, A-2
Power Over pressure alarm, 4-10
Turning on, 3-3, B-12 Primary pump head, 2-24
Power cord, 5-37, B-11 Priming, 3-10, B-25
Power receptacle, 2-11, B-11 Priming problems, 4-15
Power requirements, A-1 Secondary pump head, 2-25
Power-up conditions, 3-3 Selecting the pressure unit, C-17
Pressure Setting pressure limits, C-17
Home page display, C-6 Troubleshooting, 4-17
Limit, 4-11, C-6, C-17 Under pressure alarm, 4-11
Pump operating specifications, A-2 Pump check valve leaking, 4-13
System, 4-10 4-11, C-17 Pump components, 2-24
Unit (selecting), C-17 Pump degas
Pressure ripple, A-2 See Vacuum degas assembly
Pressure slope calibration error, 4-9 Pump head waste valve
Pressure transducer, 2-8, 2-26 Leaking, 4-14
Leaking, 4-14 O-ring replacement procedure, 5-23
System backpressure, 2-26
V
Vacuum degas assembly, 2-22
Calibration, 5-10
Components, 2-22
Fails to run, 4-25
Setting operating parameters, C-18
Specifications, A-3
Troubleshooting, 4-15
Valve
See Eluent valve
See Injection valve
Version number, C-27
W
Warranty, voiding, 5-1
Waste container, 5-49
Waste lines
Blocked, 4-13
Connection, B-23
Connection to AS50, B-58
Waste valve, 2-25
Opening, 2-25, 5-18
O-ring replacement procedure, 5-23
Water samples, 3-14
Weekly maintenance, 3-24
Weight, A-1
Wellness Panel, 2-16, 5-1
Y
Yearly maintenance, 3-24
Z
Zero position (analog out), C-25
Zeroing the baseline, C-9