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March of The Proteins

The document is a song written by Dr. Kevin Ahern about protein purification techniques set to the tune of "The Ants Go Marching One by One". It describes different chromatography methods like size exclusion filtration, gel electrophoresis, and ion exchange chromatography. It also briefly mentions dialysis and encourages students to study HPLC and contact the author if they have any other questions about "protein hell".

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Dragana Modesty
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29 views2 pages

March of The Proteins

The document is a song written by Dr. Kevin Ahern about protein purification techniques set to the tune of "The Ants Go Marching One by One". It describes different chromatography methods like size exclusion filtration, gel electrophoresis, and ion exchange chromatography. It also briefly mentions dialysis and encourages students to study HPLC and contact the author if they have any other questions about "protein hell".

Uploaded by

Dragana Modesty
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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Music about proteins

Dr. Ahern (www.davincipress.com/metabmelodies.html) has created a march of proteins, inspired by The Ants
Go Marching One by One.

March of the Proteins


Oh there's a method you should know that's very huge
It's spinning round and round inside the centrifuge
The supernatant, pellet too
You choose the one that's right for you
And from there we pu-ri-fy
What's inside

To size exclude filtration is the way to go


The beads have pores small proteins can go in you know
The largest ones, they come out fast
The smallest ones eluting last
And the proteins purified
By their size

Electrons power gel e-lec-tro-pho-re-sis


The protein is denatured thanks to SDS
Proteins in a minus state
Get sorted by atomic weight
Smaller ones in speedy mode
To the anode

Ion exchange is special chromatography


To switch cations, you must have a minus bead
Upon this bead, the proteins bind
They're positive, not any kind
And the others wash right through
Out to you

Oh my this song has given you a mighty list


Perhaps we'll just skip over ol' dialysis
So study HPL and C
If you have questions, talk to me
You will get through protein hell

Music about proteins


Here it is one more music from Dr. Kevin Ahern (www.davincipress.com/metabmelodies.html), this time about
proteins. It was based on the Oh Little Town of Bethlehem.

http://www.mediafire.com/?hr9d8wj1501brqn
Oh Little Protein Molecule
Instructor sings
Oh little protein molecule
You're lovely and serene
With twenty zwitterions like
Cysteine and alanine

Everyone sings
Your secondary structure
Has pitches and repeats
Arranged in alpha helices
And beta pleated sheets

Instructor sings
The Ramachandran plots are
Predictions made to try
To tell the structures you can have
For angles phi and psi

Everyone sings
And tertiary structure
Gives polypeptides zing
Because of magic that occurs
In protein fol-ding

Instructor sings
A folded enzyme's active
And starts to catalyze
When activators bind into
The allosteric sites

Everyone sings
Some other mechanisms
Control the enzyme rates
By regulating synthesis
And placement of phosphates

Instructor sings
And all the regulation
That's found inside of cells
Reminds the students learning it
Of pathways straight from hell

Everyone sings
So here's how to remember
The phosphate strategies
They turn the GPb's to a's
And GSa's to b's

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