Operation Manual for

SoftWare
M. Wave Basic

PLEASE READ THIS MANUAL CAREFULLY BEFORE OPERATION

3, Hagavish st. Israel 58817 Tel: 972 3 5595252, Fax: 972 3 5594529 [email protected]

MRC.VER.01-1.12
 Contents
1. Function at a Glance . 1
1.1Main Function. 1
2. Install Software .... 2
2.1 System Requirement ... 2
2.2 Install M.Wave Basic2
2.3 Uninstall M.Wave Basic . 3
2.4 Run M.Wave Basic ..3
2.5 Set Communication Port... 4
2.6 Set Users Information ........................................................................................4
2.7 Connect to/Release PC... 4
3. Software Introduction..6
3.1 Main Interface.........6
3.2 Menu Bar & Tool Bar......... 7
4. Operation.9
4.1 Quantitative Test.....9
4.1.1 Measure... 9
4.1.2 Modify a Test Result 11
4.1.3 Delete a Test Result 11
4.1.4 Name a Sample 11
4.2 Kinetics....12
4.2.1 Measure a Sample12
4.2.2 Switch Display Mode13
4.2.3 Magnify the Selected Zoom 13
4.2.4 Rescale the Coordinates13
4.2.5 Switch the Display status13
4.2.6 Search Peaks13
4.3 Photometry..15
4.3.1 Measure15
4.3.2 Modify a test result..16
4.3.3 Delete a test result..16
4.3.4 Name a sample...16
4.3.5 Switch the photometric mode....16
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1. Function at a Glance
Well introduce you the main functions of M.Wave Basic in this chapter

1) Quantitative Analysis
We provide you 2 methods to establish a Standard Curve:

Coefficient Method
If you have known the coefficients of a curves equation, you can input them directly,
then the curve displays on the screen. You can measure unknown samples by the
curve.

Set up a curve by standard samples
You can use at most 20 standard samples to set up a new curve. 3 ways are under
your choice to fit the curve, which are Linear, Linear through zero and Quadratic.

2) Kinetics

You can choose the Time Intervals0.51.0, 2.0, 5.0, 10.0, 30.0 or 60.0 Seconds

You can choose different Photometric Mode to display the curve.
(Transmittance-Time & Absorbance-Time)

3) Photometry Measurement

The test result can be displayed in Transmittance Mode and Absorbance
Mode.

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2. Install Software
Well show you how to install M.Wave Basic on you PC in this chapter.

2.1 PC System Requirement

Pentium or above PC

CD-ROM

USB Port (At least 2)

32 MB Memory(256 MB or Above is strongly recommended

50 MB or above Hard-disc Space

Micorsoft Windows 2000 or Windows XP

2.2 Install M.Wave Basic

Put M.Wave Basic disc in the CD-ROM.

Open the files on the disc.

Double Click Setup.exe to begin the installationa welcome interface will appear on
the screen (Fig.2-1). Click Next.

Input the users information, then click Next.Fig. 2-2.

Choose install path, then click NextFig. 2-3.

Choose Shortcut file folder, then click Next Step.Fig. 2-4. All the setting information
will be displayed on the screen(Fig.2-5, click Next, all the files will be copied to the
Hard Disc.

A USB setup interface (Fig.2-6) will appear after last step. Click Install to install USB
driver. Click OK to confirm in the new frame. (Fig.2-7)

Click Finish to finish the installation. (Fig.2-8)

Fig. 2-1 Welcome Interface Fig. 2-2 Users Information

Fig. 2-3 Install Path Fig. 2-4 Choose Shortcut Folder

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Fig. 2-5 Display Users Information Fig.2-6 Install USB Driver

Fig.2-7 Finish Install USB Driver Fig. 2-8 Finish Total Install

2.3 Uninstall M.Wave Basic

Please find the Uninstall file in the listed path on your PCs desktop.

StartAll FilesM.Wave BasicUninstall M.Wave Basic 1.0 Click the last
menu, then click Next to Uninstall.(Fig. 2-9

After all the files are removedclick Finish to end. (Fig. 2-10

Fig. 2-9 Uninstall Information Fig. 2-10 Finish Uninstallation

2.4 Run M.Wave Basic

Two ways are under your choice to run M.Wave Basic.
1) Double click the icon on your PCs desktop.
2) StartAll filesM.Wave BasicM.Wave Basic1.0.

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2.5 Setup Communication Port

After M. Wave Professional being startedclick View then Option on the Menu Bar.
Click Search to find the communication port in the following frame(Fig 2-11 then
click OK to confirm(Fig.2-12). Click the icon in the Tools Bar to connect to PC.

Fig.2-11 Search the communication port

If you choose the option of Connect to Spectrophotometer When Startthen the

next time you run the software, the instrument will connect to the PC automatically.

Fig. 2-12 Setup Communication Port

2.6 Set Users Information
Click Users Information in Fig.2-12, then Input your information, click OK to confirm.
(Fig. 2-13)

Fig.2-13 Set Users Information

2.7 Connect to or Release PC.

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Click the icon in the Tools Bar to connect to or release PC.

If you can find the model name of the instrument in the lower corner of the screens
Left area (Status Bar), then the instrument connects to the computer successfully. Or
it displays Unconnected in this area. (Fig.3-1)

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3. Software Introduction
We will introduce you the total running environment of M.Wave Basic in this chapter.

3.1 Main Interface

This is the main interface after start.Fig. 3-1

Fig. 3-1 Main Interface

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3.2 Menus Bar and Tools Bar

Menus Bar and Tools Bar provide two different ways to operate this software.

You can finish all the operation through Keyboard or Mouse.

Most of the functions can be finished through the Shortcut Bar.

Main Class1 Tool

Class 2 Submenu Function
Menu Submenu Bar
Quantitative Analysis Start a New Quantitative Test
Kinetics Analysis Start a New Kinetics Test
New
Wavelength Scanning Start a New Wavelength Scan Test
Photometry Test Start a New Photeometric Test.
File Open Load a Saved File
Close Close the Current Test
Save Save the Current Test
Print Print Test Report
Exit Exit M.Wave Basic
Standard Display/Hide Tools Bar
Tools bar Display/Hide
Spectrum
View Spectrum Process Tools Bar
Status bar Display/Hide Status Bar
Options Set System Parameters
Connect/Release Connect to or Release the PC
Turn On/Off W
Turn On/Off W Lamp
UV-Spectro Lamp
photometer Turn On/Off D2
Turn On/Off D2 Lamp
Lamp
Goto Wavelength Set Wavelength
Start Test
Cancel Cancel Current Test
Modify Re-measure the Current Sample
Operation
Delete Delete Current Test Data
Zero/Full Set Zero and 100%T
Setting Set Test Parameters
Back See the Spectrums Upper Status
Forward See the Spectrums Latter Status
Abs. Set the Photometric Mode to Abs.
Display Mode
Tran. Set the Photometric Mode to Trans.
Zoom Range Reset Scales of the Coordinate
Spectrum Zoom Zoom Window Magnify the Selected Area
Zoom All Resume the Original Status
Peak Auto Search the Spectrum Peak
Height Setup Set the Height of the Peak

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Close Close the Current Test

Multi Windows Display in a

Cascade
Cascade
Window
Multi Windows Display in a
Tile Vertically
Vertically Tile
Multi Windows Display in a
Tile Horizontally
Horizontally Tile.
Help About Information About M.Wave Basic
Table 3-1 Menus and Shortcut Icons

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4. Operation
We will introduce you how to use M.Wave Basic

4.1 Quantitative Test

M.Wave Basic use Standard Curve to test the samples concentration at a fixed
wavelength point.

4.1.1 Measure
1. Click the icon in the Tools Bar to create a new Quantitative Test. (Fig. 4-1

Fig. 4-1 the Main Interface of Quantitative Test

2. Click the icon in the Tools Bar to set the parameters of Quantitative Test. (Fig.
4-2
D

F
B

C
E

Fig. 4-2 Parameters Setting Interface of Quantitative Test

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3. Input the wavelength you want in Area B (Fig.4-2).

4. Choose the Concentration Unit in Area C (Fig.4-2).
5. Choose curve fitting method in Area D (Fig.4-2)
6. Set up Standard Curve.
Two methods are under your choice to create a new curve.

Method 1 Coefficient
(1) Click Coefficient option in Area E. (Fig.4-2)
(2) Input the curve equations coefficients in corresponding frames in Area E.
(Fig. 4-2.
(3) Click OK to finish setting.

Method 2 Use Standard Samples to create a new curve

(1) Click Standard Sampling option in Area E (Fig.4-2).
(2) Choose the quantity (20of the standard samples in Area E. (Fig.4-2)
(3) Input the standard samples concentration in corresponding frame in Area F.
(Fig.4-2)
(4) Click OK to finish setting.
(5) Pull the Reference into the light path and click the icon then the system
will move the wavelength to the one you just input, it blanks then.(For the
model of V-1100D, the system will prompt you to set the wavelength at
the point you want.)
(6) Pull 1# standard sample into the light path and move the cursor on the first
frame of Abs. in Area C. (Fig.4-3) The samples Absorbance value will
appear in this frame after double click.
(7) Measure other standard samples in the same way as step (6) shows. The
curve displays automatically in Area B (Fig.4-3) when finish.

Fig. 4-3 Create Standard Curve and Measure

Note: You can modify the curves coordinates by clicking the icon . (Fig. 4-4.
You can also save the curve by clicking , and you can reload it by clicking
next time.

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Fig. 4-4 Re-scale the Curves Coordinates

7. Pull the reference into the light path, click the icon again to set Zero and Full scale.
Pull the unknown concentration sample into the light path, click the icon in the
tools bar to test. And the test result will display in the data sheet in Area A. (Fig.4-3)
8. Finish all the test following the way as step 8 does.

4.1.2 Modify a Test Result

1. Move the cursor on the data you want to amend and click.
2. Pull the sample into the light path.
3. Click the icon in the Tools Bar to re-measure the sample, and the new value will
replace the old one.

4.1.3 Delete a Test Result

1. Move the cursor on the data you want to delete and click.
2. Click the button in the Tools Menu to delete the data.

4.1.4 Name a Sample

1. Move the cursor on the sample line you want to name.
2. Input the sample name after double click the frame, then press Enter to confirm.

4.2 Kinetics
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Well introduce you how to measure a samples change of Absorbance or Transmittance

over a selected period of time.

4.2.1 Measure a Sample

1. Click the icon to begin a new test of Kinetics.Fig.4-5.

Fig.4-5 Main Interface of Kinetics

2. Click the button of to set the parameters (Fig.4-6

Fig.4-6 Set Parameters of Kinetics

3. Choose Photometric Display Modeinput the wavelength, Delay Time, Total Time,
Intervals and coordinates values
4. Click OK to finish and cancel setting.
5. Pull the reference into the light path and click icon in the Tools Bar to set zero
and full scale.(For the model of V-1100D, the system will prompt you to set the
wavelength at the point you want)
6. Pull the sample into the light path and click the button of to begin the test.Fig.
4-7you can click icon in the Tools Bar to cancel at any time.

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Fig. 4-7 Kinetics

4.2.2 Switch the Display Mode

Click the icon or in the Tools Bar to choose the photometric mode you want.
(Abs.-Time, Tran.-Time)

4.2.3 Magnify the Selected Zoom.

Click the icon in the Tools Bar, choose the area you want to magnify, then
youll see the magnified one, and the Mouses cursor turns into the form of + .
Click the icon again to cancel the current status.

4.2.4 Rescale the coordinates

Click the icon in the Tools Bar, input the new value you want in the new frame
(Fig. 4-8) and click OK to confirm. Then the curve with new coordinates will display
on the screen.

Fig.4-8 Rescale the Coordinate

4.2.5 Switch the Display Status

Click the icon or to display the curves upper or latter status, click to
recover the original status.

4.2.6 Search Peaks

Click the icon in the Tools Bar to search the peaks, they will be labeled
automatically on the curve, and the peaks height will be listed in the Right Frame.
(Fig.4-7)
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Click the icon in the Tools Bar. You should input the peaks height that you want
to find in the new frame and click OK. (Fig.4-9) Then the peaks whose height is
lower than the inputted value will be automatically labeled.

Fig.4-9 Set peaks height

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4.3 Photometry
This is a basic function of the spectrophotometer. You can measure the
photometric value in a fixed wavelength.

4.3.1Measure
1) Click the icon of in the Tools menu to begin a new photometry
test.(Fig.4-18)

Fig.4-18 The main Interface of Photometry

2) Click the icon of in the Tools Menu and input the wavelength value you
need.(Fig.4-19)

Fig.4-19

3) Turn the wavelength knob and set the wavelength at the point you just input.
Then click OK to confirm.

4) Pull the Reference in the light path, click the icon of to set zero.

5) Pull the sample in the light path, click the icon of to begin the test. And the
test result will display in the data sheet. (Fig. 4-20)

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Fig.4-20 test result

4.3.2 Modify a Test Result

1. Move the cursor on the data you want to amend and click.
2. Pull the sample into the light path.
3. Click the icon in the Tools Bar to re-measure the sample, and the new value will
replace the old one.

4.3.3 Delete a Test Result

1. Move the cursor on the data you want to delete and click.
2. Click the button in the Tools Menu to delete the data.

4.3.4 Name a Sample

1. Move the cursor on the sample line you want to name.
2. Input the sample name after double click the frame, then press Enter to confirm.

4.3.5 Switch the Photometric Mode

Click the icon of or to switch the photometric mode freely.

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