Scribd Logo
Upload

Welcome to Scribd!

  • 10 views

    Marina Eloi, Daniela Vargas Horvath, João Carlos Ortega, Monica Simon Prado, Luis Eduardo Coelho Andrade, Et Al

    Uploaded by

    Sitha Wisesa
    1) The study assessed vitamin D levels in 199 SLE patients and found that 71.4% had vitamin D deficiency, with levels below 30 ng/mL. 2) Vitamin D levels were significantly lower in patients with more severe SLE disease activity. Serum 25(OH)D levels were significantly different between patients with inactive/mild SLE versus those with severe disease activity. 3) However, measuring free and bioavailable vitamin D did not provide additional information about disease activity levels. While total 25(OH)D was associated with SLE severity, free and bioavailable fractions showed no such association.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd

    Marina Eloi, Daniela Vargas Horvath, João Carlos Ortega, Monica Simon Prado, Luis Eduardo Coelho Andrade, Et Al

    Uploaded by

    Sitha Wisesa
    10 views2 pages
    1) The study assessed vitamin D levels in 199 SLE patients and found that 71.4% had vitamin D deficiency, with levels below 30 ng/mL. 2) Vitamin D levels were significantly lower in patients with more severe SLE disease activity. Serum 25(OH)D levels were significantly different between patients with inactive/mild SLE versus those with severe disease activity. 3) However, measuring free and bioavailable vitamin D did not provide additional information about disease activity levels. While total 25(OH)D was associated with SLE severity, free and bioavailable fractions showed no such association.

    Original Description:

    sle dan vit d

    Original Title

    je sero1

    Copyright

    © © All Rights Reserved

    Available Formats

    DOCX, PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    1) The study assessed vitamin D levels in 199 SLE patients and found that 71.4% had vitamin D deficiency, with levels below 30 ng/mL. 2) Vitamin D levels were significantly lower in patients with more severe SLE disease activity. Serum 25(OH)D levels were significantly different between patients with inactive/mild SLE versus those with severe disease activity. 3) However, measuring free and bioavailable vitamin D did not provide additional information about disease activity levels. While total 25(OH)D was associated with SLE severity, free and bioavailable fractions showed no such association.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    10 views2 pages

    Marina Eloi, Daniela Vargas Horvath, João Carlos Ortega, Monica Simon Prado, Luis Eduardo Coelho Andrade, Et Al

    Uploaded by

    Sitha Wisesa
    1) The study assessed vitamin D levels in 199 SLE patients and found that 71.4% had vitamin D deficiency, with levels below 30 ng/mL. 2) Vitamin D levels were significantly lower in patients with more severe SLE disease activity. Serum 25(OH)D levels were significantly different between patients with inactive/mild SLE versus those with severe disease activity. 3) However, measuring free and bioavailable vitamin D did not provide additional information about disease activity levels. While total 25(OH)D was associated with SLE severity, free and bioavailable fractions showed no such association.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    of 2

    PLOS ONE | DOI:10.1371/journal.pone.

    0170323
    Journal Reading
    Name : Rositha Ratna Wisesa
    Moderator : Anna Tjandrawati
    Day/date : Wednesday, May 17th 2016
    Department of Clinical Pathology, Medical Faculty of Padjadjaran University / Dr. Hasan Sadikin
    Hospital, Bandung

    25-Hydroxivitamin D Serum Concentration, Not Free and Bioavailable Vitamin D, Is


    Associated with Disease Activity in Systemic Lupus Erythematosus Patients
    Marina Eloi, Daniela Vargas Horvath, Joo Carlos Ortega, Monica Simon Prado, Luis
    Eduardo Coelho Andrade, et al

    Introduction
    Systemic lupus erythematosus (SLE) is a chronic autoimmune disease that can affect
    almost any organ system. One possible explanation for the association between SLE and
    vitamin D deficiency is the immunological abnormalities in SLE. The correlation between
    high disease activity in SLE with low vitamin D serum concentrations has been reported, but
    these results are controversial. The aim of this study is to assess the prevalence of vitamin D
    deficiency in a cohort of patients with SLE and examine the association between total, free
    and bioavailable vitamin D serum measurements with disease activity.

    Methods
    From 350 patients who attend at the Rheumatology Outpatient Clinics in period of time
    February 2014 to January 2016, 199 were diagnosed with SLE according to the American
    College of Rheumatology (ACR) 1997 classification criteria and met the inclusion criteria.
    Anthropometric, demographic and clinical data collected from the electronic medical charts
    or clinical interview included age, sex, race, weight, height, disease duration and medication
    in use.
    Blood samples were collected by intravenous puncture and serum aliquots were stored at
    -80C for the biochemical analyses described below. The measurements of 25-(OH)D serum
    were performed on a Siemens ADVIA Centaur apparatus using chemiluminescence
    technique. Vitamin D binding protein (DBP) was measured by monoclonal antibody ELISA
    (Cloud-Clone Corp. Kit-USCN Life Science Inc.) The coefficient of variation (CV%) for
    DBP measurement is 4.8%.
    For prospective analyses, quantitative variables were compared using ANOVA and
    categorical variables were analyzed using Chi-square test with Bonferroni correction for
    multiple comparisons. Statistical analyzes were performed using SPSS software version 17.0
    (Chicago, IL) significance level was set as p< 0.05.

    Results
    The age of 199 SLE subjects were 37.2 11.1 years old (range 26 to 48 years old) and
    mainly women (96%). Of those 142 subjects (71.4%) had 25(OH)D serum concentrations
    below 30 ng/mL and significantly higher than that seen for healthy controls (p<0,001). Only
    57 subjects (28.6%) had 25(OH)D serum concentrations exceeding 30 ng/mL (vitamin D
    sufficiency). Vitamin D serum concentration was demonstrated a statistically significant
    difference between inactive disease (SLEDAI = 0) or light activity (SLEDAI 15) and severe
    activity (SLEDAI 20) (p<0.001; Tukey test).
    The two groups (25(OH)D < 30 ng/mL versus 30 ng/mL) differ significantly in the propor-
    tions of disease activity (p = 0.001). The proportion of patients with vitamin D sufficiency
    (25(OH)D 30 ng/mL) is significantly higher in the groups SLEDAI 0 and 15 when
    compared to 25(OH)D values below 30 ng/mL (p= 0.003). In patients with severe activity
    (SLEDAI 20), the proportion of patients with 25 (OH)D serum concentration lower than 30
    ng/mL was significantly higher as compared to values 30 ng/mL (p= 0.026).
    The Potential associations between vitamin D status and season, body mass index (BMI),
    Bone Mineral Density (BMD) and use of medication were also tested. There was no
    statistically significant difference in the mean serum concentrations of 25(OH)D (p= 0.179),
    free and bioavailable vitamin D (p= 0.041) between the different seasons. The concentration
    of 25(OH)D serum were not significantly associated with the disease duration or BMI. Both
    free and bioavailable vitamin D was also not associated with those variables.

    Discussion
    This study observed a very high prevalence of vitamin D deficiency and insufficiency
    among SLE patients. Only 28.6% of the patients were vitamin D sufficient (serum 25(OH)D
    30 ng/mL). Vitamin D deficiency was significantly more frequent in SLE patients than in
    healthy controls. They have also found that disease activity in SLE was associated with lower
    25(OH)D serum concentration. On the other hand, measuring DBP with a monoclonal ELISA
    in this clinical setting has not added information to the status of vitamin D: both free and
    bioavailable fractions of 25(OH)D did not differ between the various categories of SLE
    disease activity.
    It was recently demonstrated that DBP measurements using monoclonal ELISA are biased
    by DBP genotype and race. Vitamin D binding protein values varied significantly when
    measured by monoclonal or polyclonal ELISA or tandem mass spectrometry and that had
    implication on free and bioavailable vitamin D calculations. Vitamin D supplementation was
    associated with increased 25(OH)D serum concentrations. About 35% of patients using
    cholecalciferol 400 to 1000 IU/day do not seem to be sufficient to ensure adequate levels of
    vitamin D and still presented vitamin D deficiency or insufficiency in these patients.

    Conclusion
    This study have demonstrated a very high frequency of vitamin D deficiency and
    insufficiency among patients with SLE. In this clinical setting, the disease activity was
    associated with lower serum concentrations of 25(OH)D. Vitamin D binding protein
    measurements with monoclonal ELISA and free and bioavailable vitamin D calculations did
    not differ among different categories of SLE disease activity.

    You might also like