Curriculum Vitae

Hernani DM Cualing, MD

Hernani Del Mundo Cualing MD

Address: 18804 Chaville Rd., Lutz, Florida 33558
Telephone: 813-480-7849 (cell phone and blackberry )
813-909-4866 (fax )
Emails: [email protected]
[email protected]
[email protected]
Consultation Specialty: Hematopathology, Cutaneous Hematopoietic Lesions
Business: IHCFlow, Inc.
Website: WWW.IHCFLOW.com
Trademark: VirtualFlow IHCFlow™ Cytometer
Affiliation: Cutaneous Lymphoma Study Group, principal, USF/ Moffitt Cancer Center
Associate Professor, Department of Pathology and Cell Biology
University of South Florida College of Medicine
University of South Florida

12901 Bruce B. Downs Blvd.

Doctorate Education, Residency, Fellowship:

1970.1974 University of the Philippines

Quezon City, Philippines
B.S. Pre-Medicine
1974-1978 University of the Philippines School of Medicine
Manila, Philippines
M.D.
1987.1990 Resident in Anatomic and Clinical Pathology
Nassau County Medical Center, SUNY at Stony Brook Teaching Hospital
East Meadow, NY

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 1990-1991 Chief Resident in Pathology and Surgical Pathology Fellowship, Nassau
County Medical Center under Dr. Vincent Palladino
SUNY at Stony Brook Teaching Hospital
East Meadow, NY

1991.1992 Hematopathology Fellow of Dr. R. Neiman and A. Orazi

Indiana University Medical Center
Riley Children’s Hospital
Indianapolis, IN

Post Graduate Training:

1978.1979 Rotating Internship- Pediatrics, Surgery, Medicine
Capiz Emmanuel Hospital
Roxas City, Philippines

1979-1980 Preventive Medicine Rural Training

University of Philippines Outreach Program
Dao, Panay Island, Philippines

1980-1982 Resident in General Surgery, University of the Philippines

Cardinal Santos Memorial Hospital
Greenhills, Manila, Philippines

1982-1985 Microbiology and Genetics, Post Doctorate MS Courses

University of Illinois at Chicago
Chicago, Illinois, USA

1985-1987 Research Fellowship in Membrane Tumor Biochemistry

Dana-Farber Cancer Institute under Dr. Emil Frei III Laboratory
Harvard Medical School
Boston,MA

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Awards and Honors:

1969-1971 Scholastic Delegate to Physics, Chemistry, Biology Interscholastic Competitions

Academic Excellence-Top Academic Rank, Section Class # 1 of 26 classes
Senior High School, Ramon Magsaysay High School,
Manila, Philippines

1976-1977 President of Medical Student Society

University of the Philippines College of Medicine
Manila, Philippines

1989-1990 First Prize Award, Resident Presentation in Pathology,

“Zell Ballen” Patterns in Organ of Zukerkandl
Nassau Society of Pathologist
East Meadow, NY

1990-1991 Chief Resident, Department of Pathology

Nassau County Medical Center, SUNY at Stony Brook Teaching Hospital
East Meadow, NY

1992-1993 Finalist, American Society of Clinical Pathologist

Spring Resident Presentation Competition
Boston, MA

1994.1995 Biomedical Engineering Seed Grant Award-Top Score

1994.1995.1.1.1 I Image Analysis of Lymphomas
University of Cincinnati University- Seed Grant Competition
Cincinnati, Ohio

1997.2000 Biomedical Engineering Grant Award

“High Order Decision Theoretic Diagnosis of Lymphomas”
Whitaker Foundation ( National )

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2000-2001 Stratmore’s Who’s Who of North America

2003-2008 Marquis Who’s Who, 58th, 60th, 61st, in North America

2006-2008 Marquis Who’s Who, 6th Edition in Science and Engineering,

Marquis Who’s Who, 9th Edition in Medicine & Health

2003-2004 Awardee- Top Overseas Researcher

University of the Philippines College of Medicine
Medical School Alumni Association
Twenty-Five Year Homecoming Celebration, Manila, Philippines

2006 First Prize Research Presentation by Mentored resident

(Farah Khalil- Bone Marrow Quantitation )
Florida West Coast Assoc. of Pathologists/Pathology Alumni Assoc.
Longboat Key Conference, FL

2004-2005 Best Teacher of the Year Award

Awarded by AP/CP Pathology Residents
Department of Pathology
University of South Florida, Tampa, FL

2004.2007 Consultant: Immunohistochemistry and Histology

Instrumentation
Council of Advisors for Health Care
New York, NY

2007.2008 Panelist: Small Business Innovations Research

(SBIR/STTIR) Grant
National Science Foundation
Bethesda, MD

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 2007.2008 Mentor: Mentorship for Young scientist
International Society of Analytical Cytometry
Clinical Cytometry Society
Chicago, IL

2008 Consultant Adviser on Cutaneous Lymphomas

Advisory Board on Health Care
Eisai Corporation, Japan and USA

2007 FIRST Prize Research Retreat “Yoshinori Takahashi, D.

Coppola, N. Matshushita, H. Cualing, M. Sun, Y. Sato, C.
Liang, J. Jung, J, Cheng, J. Mule, WJ Pledger, Hong Gang Wang.
Bif-1 interacts with Beclin 1 through UVRAG and regulates
autophagy and tumorigenesis” 2007 Moffitt Scientific Retreat
publication, Blaise Alfano Conf.Center, Feb 24, 2007

2008 FIRST Prize Awardee Fellow Research Y.Takahashi,

D.Coppola,
N. Matshushita, H. Cualing, M. Sato, C. Liang, J. Mule, WJ
Pledger, Hong Gang Wang. H. Lee Mofttitt Cancer Center
Fellow Research Symphosium- Bif-1 in autophagy and tumorigenesis,
Stabile Bldg.
Basic Science Division, May 19, 2008

Licensures and Certifications:

2002-2010 Florida Medical License # ME 84854, Expiration Jan 31, 2012, Active, Clear
1998 4th International Symposium on Predictive Oncology and Therapy, Nice, France
1996 Lymph Node Pathology: ASCP Winter Seminar Certification, Orlando, FL

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 1994 T-cell Lymphomas-Comparison of Eastern and Western Experience: Hong Kong
1992 Ohio Medical License #35-06-4174, Inactive
1992 Diplomate of American Board of Pathology, Hematology Subspecialty
1992 Indiana University School of Medicine, Hematopathology Fellowship Training
1991 Diplomate of American Board of Pathology, Anatomic and Clinical Pathology
1991 Lymph Node Pathology in the 90s (Bharat Nathwani), Washington DC
1990 New York Medical License #184512, Inactive
1990 Iowa Medical License #27672, Inactive
1991 ACLS(Advanced Cardiac Life Support) Certified, NY
1987 Lymphomas, Diagnosis from both sides of the Atlantic: Karl Lennert, Nassau, NY
1981 ECFMG Certification #322-877-2
1978 National Medical Licensure Certification, Philippines

Academic Appointments and Employment

1990-1991 Chief Resident

Nassau Country Medical Center, SUNY Stony Brook Campus
East Meadow, NY

1992-2001 Assistant Professor, Clinical

Attending Staff of Hospital
Department of Pathology and Laboratory Medicine
University of Cincinnati Medical Center, Cincinnati, Ohio

1994-2002 Course Director

Diagnostic Hematopathology
Year IV Medical Student Elective- UC-150146
University of Cincinnati College of Medicine

1993-1997 Medical Director

Hospital Phlebotomy Service
University of Cincinnati Medical Center

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 1993-2002 Consultant: Hematopathology/Hematology Attending
Adams County Hospital Affiliate, Univ of Cincinnati
Cincinnati, Ohio

2001-2002 Co-Director, Hematopathology Fellowship Program

University of Cincinnati Department of Pathology and Lab Medicine
Cincinnati, Ohio

2002- Associate Professor, Anatomic and Clinical Hematopathology

University of Cincinnati College of Medicine
Cincinnati, Ohio

2002-present Associate Professor, Clinical

University of South Florida, Department of Pathology and Cell Biology
Tampa, Florida

2002-present Associate Member and Attending Staff,

Department of Oncologic Sciences, Division of Hematopathology and Laboratory
Medicine, Moffitt Cancer and Research Institute
Tampa, Florida

Clinical appointments

1987.1991 Resident in Anatomic and Clinical Pathology,

Nassau Country Medical Center, SUNY Stony Brook Campus
East Meadow, NY

1990.1991 Chief Resident

Nassau Country Medical Center, SUNY Stony Brook Campus
East Meadow, NY

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1991-1992 Clinical Fellow in Hematopathology
Indiana University Medical Center
Riley Children’s Hospital
Indianapolis, IN

1992- 2002 Surgical Pathologist Attending Staff

Department of Pathology and Laboratory Medicine
University of Cincinnati Medical Center and Holmes Hospital
Cincinnati, OH

1993- 2002 Consulting Hematopathology Staff

Department of Pathology, Veterans Administration Medical Center
Cincinnati, OH

1996- 2002 Consulting Hematopathology Staff, Adams County Hospital

Adams County, OH

2000-2002 Medical Director

Flow Cytometry and Immunology Division
Health Alliance of Greater Cincinnati, Inc.
Cincinnati, Ohio

2002-present Hematopathology Attending Staff,

Department of Interdisciplinary Oncology/ Oncologic Sciences
Moffitt Cancer Center and Research Institute
Univ of South Florida College of Medicine Clinics,
Tampa, Florida
Heme Malignancies Pathologist
Lymph Nodes, Extranodal Tissue and Bone Marrow Diagnostic Service
Duties: Signs out Flow cytometry, coagulation molecular testing, peripheral blood, bone
marrow, lymph nodes, spleen, cutaneous lymphomas, GI Renal Lung Brain and other

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 extranodal lymphoma work up biopsy, core biopsy specimens of suspected hematologic
malignancies, Reactive and Non neoplastic Hematopathology
Department Case Load: 3800 bone marrows, about 600 lymph nodes and FNAs, 1800
flow cytometry, 600 molecular and blood studies including CISH, FISH, and 22,000
immunohistochemistry tests per year.

2005-present Expert Consultant on Lymphomas and Cutaneous lymphomas

Contract consultant for lymphomas and leukemias involving lymph nodes and
cutaneous and other extranodal tissues
Quest Diagnostics, Fowler Av., Tampa, Florida

2009-present Expert Consultant on Cutaneous Lymphomas

Dermpath Diagnostics, Ameripath, Tampa, FL

Administrative:

2007 Course Co-Director: First Annual Cutaneous Lymphoma seminar, MCC

Cutaneous Cooperative Group: Dr. Frank Glass and Dr. Lubosh Sokol
University of South Florida, Tampa FL

2008 Course Director, 2nd Annual Cutaneous Lymphoma Seminar

With Patients Participation
Lake Buena Vista Resort,Orlando, Florida ( April 19 and April 20 )
Faculty from Boston, Rhode Is. and Florida : Organized the logistics, topics, scope and
faculty roles plus facilitated the direct participation of three lymphoma patients as
speakers.

2002.2008 Medical Director of Immunohistochemistry/Histology

Division of Pathology, Hematopathology and Laboratory Medicine
Department of Interdisciplinary Oncology,
H. Lee Moffitt Cancer Center and Research Institute,
Tampa, Florida.

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 Role: Guide strategic direction as well as supervise day to day quality assurance issues in
histology and immunohistochemistry, chromogenic and fluorescent in situ hybridization,
and special stains supervising 8 FTE histotechnologists and 2 Lead Techs and 1
supervisor with a total of 22,000 annual antibody and in situ hybridization panels.
:Quality Assurance and CAP survey reporting results
:Signed off on Histo-Immunology procedures
: Monitored technologist CAP inspection preparation
: Introduced panels and monitored the more than 135 monoclonal antibodies used in
Surgical and Hematopathology Divisions
: Performed Quality and Cost Effectiveness evaluations for histology and automated
immunohistochemistry instruments
5 Ventana Nexes and 2 Benchmarks, Leica Instruments

2006-2010 Program Director,

Hematopathology Fellowship Training Program
USF- Moffitt DIO and Department of Oncologic Sciences
University of South Florida
Tampa, Florida

Role: Administrative and Teaching-10 % time allotment

Programmatic direction and maintaining Accreditation in American Council of
Graduate Medical Education ( ACGME Hematopathology Program)
2008- Directed program that obtained the 5 year ACGME Full accreditation

Recruitment of fellows
2007 cycle – evaluated 33 applicants candidates for 1 year or 2 year track
2008 cycle – evaluated 32 applicants candidates
2009 cycle- evaluated 34 applicants
Supervision of Fellows
Immediate Past fellows:

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 Supervised and advised two fellows that graduated 2007 and 2008
Current Fellows:
Supervised and advised two 2-year track fellows

:Directed the fellowship rotation: location, content, and quality

: Program Information Plan- monitored and evaluated the competency-based
training of hematopathology fellows according to ACGME approved PIF
: Assignment of mentors and direction of research projects for fellows;
: Administrative meeting with fellows regularly for assessment and feedback;
: Liason with teaching faculty regarding fellows issues
: Directs 2 Fellowship Coordinators and 1 Secretarial Assistant
: Selection and directions for the teaching faculty - 4 hematopathologists, 1
Molecular Pathologist, 2 Heme Oncologist, 2 Molecular Cytogeneticist, and 1
Pediatric Hematopathologist that are based from MCC, VA Haley Medical
Center and All Children’s Hospital.

2000.2002 Medical Director

Health Alliance of Cincinnati, Immunology
Diagnostic Immunology: Protein Electrophoresis, Serology interpretation,
Flow Cytometry Interpretation of Leukemias and Lymphomas,
Diagnostic Flow Cytometry, Health Alliance of Greater Cincinnati
Diagnostic Immunology, FISH, CISH, Health Alliance of Greater Cincinnati
Cincinnati, Ohio

Role: Oversaw the medical /scientific direction and daily quality issues
Supervised Flow Cytometrist 5-FTE in Flow Cytometry and 6 immunology
FTE technologist in immunology division
Reviews daily results including those with discrepancies and gating issues
Liason with clinicians

1993-1996 Medical Director

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 University of Cincinnati Medical Center Phlebotomy Services
Liason with Clinicians and Nursing Staff regarding Blood Drawing Issues
Cincinnati, Ohio

1992-1999 Surgical Pathology and Hematopathology Sign-out including incorporation of flow

cytometry and gene rearrangement results in reports
University of Cincinnati Department of Pathology
Cincinnati, Ohio

1992-2002 Lymphoma Frozen Tissue Bank Database Director

University of Cincinnati Pathology and Hematopathology
Cincinnati, Ohio

Service:

Committee activities:

1990-2002 Deans Residency Review Committee, Resident Member

Nassau Country Medical Center, East Meadow, NY

1994-2002 Director
Telepathology Link Committee with Adams Country Hospital
Cincinnati, Ohio

1998.2002 Member
Scientific Advisory Committee
Confocal and Image analysis instrumentation

2000-2002 Co-Chair with Paul Hurtubise, PhD

Flow Cytometry and Immunology Methods Committees
Health Alliance of Greater Cincinnati
Cincinnati, Ohio

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 2000-2002 Member
Hematopathology Fellowship committee
ACGME Accredited Fellowship, University of Cincinnati
Cincinnati, Ohio

2001.2002 Member
Health Alliance of Greater Cincinnati Bone marrow CAP compliance
Committee, University of Cincinnati
Cincinnati, Ohio

2000-2002 Hematology Sophomore Teaching Block Course Review Committee,

University of Cincinnati College of Medicine, Cincinnati, Ohio

2004-present Member, Resident Teaching Task Force Committee in Pathology

Moffitt Cancer Center, USF and DIO
Tampa, FL

2005-present Member, Graduate Medical Education Committee,

Moffitt Cancer Center GME

2006-present Pathology Teaching Committee member

Department of Pathology and Cell Biology
University of South Florida, Tampa, Florida

2006-present Graduate Medical Education Committee Member,

University of South Florida College of Medicine
USF, Tampa, Florida

2007 Moffitt Cancer Center Appointment Promotion Tenure Committee Member

Department of Interdisciplinary Oncology, Tampa, Florida

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2007 Internal Reviewer faculty for USF GME office for Pediatric Pathology
Fellowship ACGME accreditation

2007-2007 Graduate Council- University Wide, University of South Florida,

member
Fellowship subcommittee: reviewed many Thesis and PhD dissertations for
Presidential and other Scholastic University Wide awards

2007-2010 Policy Review Team Committee, member, MCC Policy Administration,

Moffitt Cancer Center and Research Institute, Tampa, Florida

2009 Internal Reviewer faculty for USF GME office for DermatoPathology
Fellowship ACGME accreditation

Community Service:

1972-1974 Coordinator- Free Medical Clinic Foundation, Premedical Student Branch,

University of the Philippines Outreach to Indigent Urban localities that
schedules medical doctors, free medicines and patients

1973-1978 Volunteers Association of the Philippines Member

Coordinated Free Cataract Surgery for indigents in Lubang Is. Performed by
the Chiefs of Ophthalmic and Neurosurgery Divisions,
Philippine General Hospital, University of the Philippines.

1973-1978 Trained in Acupuncture Medicine by Chinese Acupuncturist and conducted

Free acupuncture services to indigent rural folks, also provided acupuncture
anaesthesia to the Department of Orthopedic Surgery, University of the
Philippines for senior citizens undergoing Decubitus Ulcer debridgement and
Relief of Post Surgical Ileus

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1974-1976 Organizer and Founder of Medical Student Annual Musical and Talent
Extravaganza –“Tao Rin Pala”- University of the Philipppines College of
Medicine,Manila, Philippines

1976-1978 Organizer
Medical Student Liason for Disaster Relief Prepareness committee
University of the Philippines Office of the Dean, College of Medicine

1979.1980 Organizer, Herbal Medicine Cooperative Group of Rural “Herbolarios”

Compiled the Dao, Panay Island indigenous Flora with Medicinal Uses and
Converted the extracts to pills and emulsions for free medicines

1988-1990 Resident In Charge of Seminar Projectors and AudioVisual Aids to Speakers,

Nassau County Society of Pathologists, East Meadow, NY

1998-2002 Cutaneous Lymphoma Cooperative Group, CTCL patient education seminars

and journal clubs

2003-2004 Alumni Fund raising committee member to benefit University of Philippines

College of Medicine Library, Manila, Philippines

2005.2007 Southwest Florida Committee member for Alumni Affairs

University of the Philippines College of Medicine, Tampa, Florida

2007 Telepathology -Outreach Tumor Board Hematopathologist, for MCC

Parish Medical Center Leukemia Lymphoma Cases

2009 Cutaneous Lymphoma Foundation Patient Education Series

Cutaneous Lymphoma Overview Speaker- Use of modern modalities in early
CTCL diagnosis Feb 21, 2009, Sheraton Venue, Tampa, FL

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Professional Association Memberships:

1987- 1991 American Association for Advancement of Science

1987- 1991 Nassau Society of Pathologists, resident member
1991- present Fellow of the College of American Pathologists, sustaining member
1992 – 2002 Cincinnati Society of Pathologists, member
1992- 2002 Society for Hematopathologists,member
1993- 2002 Southwest Oncology Group, member
1993- 2000 Cutaneous Lymphoma Cooperative Group, Univ of Cincinnati
1996- 2000 Ohio State Society of Pathologists
1996- present International Academy of Pathologists-USCAP
1996- 2000 American Society of Clinical Pathologists
1998- 2002 The International Society for Optical Engineering
1999- 2002 International Society of Preventive Oncology
2002 -present Moffitt Cancer Center and Research Inst. In-residence
2002 -present American Society of Hematology
2006-2010 International Society of Analytical Cytology
2010 American Society of Tropical Medicine and Hygiene

Teaching experience:

University Courses/Advisory Role:

1991-1992 Clinical Instructor

Indiana University Medical School
Sophomore Clinical Hematology Laboratory Course

1992- 2002 Course Instructor: Lecturer

Hodgkin and Non Hodgkin Lymphomas
University of Cincinnati College of Medicine
Medical Student Sophomore Pathology Lecture series

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1992- 2002 Course Instructor: Laboratory Session
Leukemias, Anemias, Lymphomas
University of Cincinnati College of Medicine
Medical Student Sophomore Pathology Lecture series

1994- 2002 Course Director

Medical Student Diagnostic Hematopathology
Year IV – Elective Course , University of Cincinnati
Hematopathology Student Papers/Reports: Andrew Mone, Alena Goldman, Yaron
Goldman and others

1994.1995 Thesis Advisor

Masters in Computer Science
Graduate student-Thia Balachander
“Algorithms in the Image Understanding of Features of Lymphoma Cells”
Master Degree of T. Balachander on Image Analysis of Lymphoma/Leukemia cells

1997-1998 Thesis Co-advisor

PhD in Computer Science
Graduate Student- T Balachander and R. Lotlikar
Image analysis and Neural Network Diagnosis of lymphoma/leukemia Cytologic
Romanosky stained Preps

1997-1998 Project Director for Students in programming an Automatic Image Acquisition using
X,Y,Z moveable microscope stage
Whitaker Foundation funded project with Professor R. Kothari
Lymphoma Diagnosis using Artificial Intelligence

1999 and 2001 Project Director for programmatic activity with MS Engineering Student using Oncor
Image, Seed Funding from Dr. Roger Smith
2000 Diagnostic Hematology Elective Medical Student Mentor – Yaron Goldman

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2001 Medical Student Adviser- Sukjinder Singh

2001 Diagnostic Hematology Medical Student (Year IV) Electives

Mentor- Andrew Mone

2002 Diagnostic Hematology Medical Student (Year IV) Electives

Mentor- Michael Tuchfarber, Alena Goldman that generated
case reports

2003 BMS 6600 Hematology Block Course Faculty,

Lectures and Laboratory Courses
University of South Florida

2006 USF Medical Student Teaching (Year IV )

Preceptorship Interdisciplinary Oncology
College of Medicine Course: BCC8117
Hematopathology preceptorship: Jeremy Bowers , T. Condarco that generated a case
report
University of South Florida

2007 Medical Student Mentor to Michael Fuller and Elizabeth Sundean

Year IV Medical Preceptorship in Pathology; BCC8117

Other Courses and National Symposia:

Regional seminars

Mar 17, 2000 Seminar Series sponsored by Cincinnati Society for Pathologists
“Flow Cytometric Immunophenotyping of Hematologic Disorders”
Cincinnati, Ohio

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May 26, 2001 “Cutaneous Lymphoma in a Child-“ invited speaker
TriState Midwest Pediatric Pathology Conference
Sponsored by Cincinnati Children’s Hospital
Cincinnati, Ohio

April 28, 2007 Seminar Lecture: “WHO-EORT Classification of Cutaneous Lymphomas”

Co-director of 1st Annual Cutaneous Lymphoma Seminar, Sponsored by USF Health.
Cutaneous Lymphoma Foundation, Moffitt Cancer Center, Tampa, Florida targeted
Florida Dermatologist, Pathologists, Dermatopathologists and Oncologist

Sept 4, 2007 COBRE Research Rounds- Core of Biomedical Research Excellence Invited Speaker on
“Emerging Vision Analysis applicable to Tissue Bioengineering and Research”-
Sponsored by Dept of Dermatology, Boston University: Roger Williams Medical Center
with Dr. Vince Falanga host, Providence, Rhode Island

National seminars

Apr 14, 1994 Course Lecturer: “Lymphomas of the Skin and the Role of the Laboratory”
Sponsored by Abbott Pharmaceutical and Nebraska-Iowa Medical Technologist
Societies, Des Moine, Nebraska

Apr 24, 1995 Course faculty Lecturer:

Hematologic Malignancies-overview
Peripheral Blood in Hematologic Malignancies
Review for Hematology Oncology Fellow Board Examination
Sponsored by Osler Institute Review
Cincinnati, Ohio

April 28, 2007 Co-director of 1st Annual Cutaneous Lymphoma Seminar, Moffitt Cancer Center,
Tampa, Sponsored by Moffitt Cancer Center Foundation and jointly with Cutaneous
Lymphoma Foundation- Update on Skin Lymphomas

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April 19, 20 2008 Director of 2nd Annual Cutaneous Lymphoma Seminar,
Sponsored by Cutaneous Lymphoma Foundation, USF Health, Moffitt Cancer Institute
and Funded by Eisai Corporation, and other National Pharmaceutical Corporations
Lake Buena Vista Resort, Orlando Florida
This two day seminar included three patients as speakers and Faculty from International
Cutaneous Lymphoma Study Group, Harvard Medical School Faculty and attended
nationally by Dermatologist, Pathologists, Oncologists and some attendees from out of
the country.

International lectures/ Presentations:

1994, Fall “Macrosplenomegaly with hemophagocytosis in AIDS”

Presented in oral platform in XX International Congress of the International Academy
of Pathology, Queen Hospital, Hong Kong

1998 October ” Immunophenotypic Diagnosis of Acute Leukemia Using Decision Tree Analysis”
Presentation in International Academy of Pathology, Nice France,
Serialized in Archives d’Anatomie et de cytologie pathologiques: Clinical and
Experimental Pathology.

1999 (July 8-24) Hematology Course Block Lecturer and Course Director for two weeks
Lectured to 2nd year medical Students for about 30 contact lecture/ laboratory hours on
Leukemias and Lymphomas and Benign Hematology
Distributed a 168 pages course handout and 2 hour block examination
Sponsored by St. Mathews University School of Medicine
Ambergis Cay, Belize

2000 Feb 25 Grand Rounds in Pathology. “REAL Classification of Lymphomas” .

Sponsored by Univ of the Philippines Department of Pathology seminar,

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 Pedro Gil, Metro Manila, Philippines

2001 Jan 15 Invited Course lecturer on “Lymphomas and the role of immunohistochemistry and
Flow Cytometry In diagnosis”
Department of Pathology Seminar Series
University of the Philippines College of Medicine
Metro Manila Philippines

Proceedings in Research Meetings:

1. Bernal SD, Cualing HM, Elias AD. Stability and shedding of small
cell carcinoma membrane antigens: applications to detection of
antigens in marrow and serum. Lung Cancer 1988; 4:79-80.

2. Bosserman L, Tanaka I, Cualing H, Elias A,

Skarin, Dreyfuss A, Bernal S. Serum
Radioimmunoassay Detects SM1 antigen in Lung
Cancer patients. Proc Am Soc Clin Onc 5:174
Mar 1986.

3. Wright J, Cucci C, Flatow J, Rosowski A,

Cualing H, Bernal S and Frei III E. Detection of two
active Dihydrofolate Acid Reductase forms in
Squamous cancer of the Head and Neck cell lines
using monoclonal antibodies. Proc of Am Soc Clin
Onc Mar 1987.

4. Wright J, Cucci C, Rosowski A, Cualing H, Bernal S,

Koch J, Waxman D and Frei E. Dihydrofolate
Reductase from Methotrexate Resistant Human
Squamous Cell Carcinoma for Monoclonal Antibody

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 generation. Proc of Am Assoc Cancer Res
28:1175 1987.

5. Yoshinori Takahashi, D. Coppola, N. Matshushita, H.

Cualing, M. Sun, Y. Sato, C. Liang, J. Jung, J, Cheng, J.
Mule, WJ Pledger, Hong Gang Wang. Bif-1 interacts with
Beclin 1 through UVRAG and regulates autophagy and
tumorigenesis, 2007 Moffitt Scientific Retreat
publication, Blaise Alfano Conf.Center, Feb 24, 2007-
FIRST Prize awardee presentation

6. Yoshinori Takahashi, D. Coppola, N. Matshushita, H.

Cualing, M. Sato, C. Liang, J. Mule, WJ Pledger, Hong
Gang Wang. H. Lee Mofttitt Cancer Center Fellow
Research Symphosium- Bif-1 in autophagy and
tumorigenesis.FIRST Prize awardee presentation
Basic Science Division, May 19, 2008

Other CME Lectures

Aug 1, 1992 “Peripheral T-cell lymphoma presenting as Angiocentric Waldeyer’s Ring Lesions “
Clinico Pathology Conference, Sponsored by Internal Medicine
University of Cincinnati Medical Center, Ohio

Aug 9, 1994 “Lymphocytes and their Markers in the Skin “

Joint Department of Dermatology and Hematology-Oncology Grand Rounds
University of Cincinnati Medical Center, Ohio

Sept 2, 1994 “Understanding the Histologic Classification of Hodgkin’s and Non-Hodgkin’s

Lymphoma” Hematology-Oncology special seminar

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 University of Cincinnati Medical Center, Ohio

Sept 22, 1995 ”Cytophagic Histiocytic Panniculitis”

Hematology -Oncology Grand Rounds
University of Cincinnati Medical Center, Ohio

May 11, 1996 “Sea Blue Histiocytes and Lecithin Cholesterol Acyltransferase Deficiency”
Hematology Oncology Grand Rounds
University of Cincinnati Medical Center, Ohio

Nov 8, 1996 Amyloid and Enlarged Tongue”

Clinical Pathology Conference Sponsored by Internal Medicine
University of Cincinnati Medical Center, Ohio

Jan 6, 1997 “REAL (Lymphoma) Classsification”

Grand Rounds in Pathology
University of Cincinnati Medical Center, Ohio

Dec 12, 1997 “Cat Scratch Disease”

Clinical Pathology Conference Sponsored by Internal Medicine
University of Cincinnati Medical Center, Ohio

Jan 5, 1998 “WHO Classification Hematologic Diseases”

Grand Rounds in Pathology
University of Cincinnati Medical Center, Ohio

June 22,1998 “Extranodal Lymphomas”

Grand Rounds in Pathology
University of Cincinnati Medical Center, Ohio

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Jan 11, 1999 “Update of WHO Classification of Lymphoid Neoplasms”
Grand Rounds in Pathology
University of Cincinnati Medical Center, Ohio

June 13, 2000. “Ocular adnexal Lymphoproliferations and Pseudotumors”

Department of Opthalmology Grand Rounds
University of Cincinnati Medical Center, Ohio

Aug 11, 2000 “Multiple Myeloma”

Clinico Pathology Conference, Sponsored by Internal Medicine
University of Cincinnati Medical Center, Ohio

Jan 26, 2001 “Mantle cell lymphomas”

Morbidity and Mortality Conference- Sponsored by Hematology Oncology
University of Cincinnati Medical Center, Ohio

April 11, 2001 “Gene Structure and Clonality”

Immunology Rounds Sponsored by Health Alliance of Greater Cincinnati
University of Cincinnati Medical Center, Ohio

April 16, 2001 “Reactive Lymphadenopathies”

Grand Rounds in Pathology
University of Cincinnati Medical Center, Ohio

April 25, 2001 “Immunoproliferative Diseases”

Immunology Rounds Sponsored by Health Alliance of Greater Cincinnati
University of Cincinnati Medical Center, Ohio

Aug 9, 2001 “Cutaneous Lymphoproliferative Diseases”

Dermatology Grand Rounds
University of Cincinnati Medical Center, Ohio

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Aug 27, 2001 “Lymphocytes – From Biology to Diseases” ( 2 hours)
Grand Rounds in Pathology
University of Cincinnati Medical Center, Ohio

Sept 21, 2001 “Advances in Image Cytometry in Hematopathology”

Moffit Cancer Center and Research Institute Grand Rounds in Pathology
Tampa, Florida

Jan 4, 2002 “Multidimensional diagnosis in Hematopathology”

Grand Rounds in Hematology Oncology
University of Cincinnati Medical Center, Ohio

Feb 4, 2002 “Diagnostic Advances using Image Cytometry”

Grand Rounds in Pathology
University of Cincinnati Medical Center, Ohio

Oct 21, 2002 “Understanding the WHO Classification of Lymphomas”

Moffitt Hematologic Malignancies Series-
Sponsored by Hematologic Malignancies
Moffitt Cancer Center, Tampa, Florida

April 10, 2003 “T-cell Prolymphocytic Leukemia”

Interesting Case Presentation- Tuesday VA Rounds
Hematologic Malignancies
Moffitt Cancer Center, Tampa, Florida

Nov 12, 2003 “Patterns in Benign and Malignant Lymphoproliferations”

Pathology Grand Rounds Series
University of South Florida
Tampa, Florida

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Mar 2, 2004 WHO Classification of Lymphomas-B cell Malignancies
Pathology Grand Rounds Series
University of South Florida
Tampa, Florida

Apr 7, 2004 WHO Classification of Lymphomas-T cell Malignancies

Pathology Grand Rounds Series
University of South Florida
Tampa, Florida

March 10, 2006 WHO/EORTC Cutaneous Lymphoma- From Biology to Disease-Update

Pathology Grand Rounds Series
University of South Florida
Tampa, Florida

April 14, 2006 Functional microanatomy and Diseases of the Spleen- Biology to Disease Series
Pathology Grand Rounds Series
University of South Florida
Tampa, Florida

Oct 13, 2006 Aggressive cutaneous peripheral T cell lymphomas and CD30 lymphomas
Pathology Grand Rounds Series
University of South Florida
Tampa, Florida

Nov 16, 2007 Update on Cutaneous Lymphomas- What’s New and What is Revised
Pathology Grand Rounds Series
University of South Florida
Tampa, Florida

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Nov 20, 2007 B-cell lymphomas- Biology to Disease Series
Pathology Grand Rounds Series
University of South Florida
Tampa, Florida

Nov 30, 2007 T-cell lymphomas- Biology to Disease Series

Pathology Grand Rounds Series
University of South Florida
Tampa, Florida

Residency Fellowship Directed Unknown Slides and Diagnostic Workshops

May 19, 2004 Hemepath unknowns slides resident conference-Preceptor

June 16, 2004 Hemepath unknowns slides resident conference-Preceptor

July 21, 2004 Lymphocytes-from Biology to Diseases.

August 18, 2004 Major Patterns of Reactive Lymph node Hyperplasia and Cases
Hemepath unknowns slide resident conference-Preceptor
Sept 1, 2004 Peripheral T cell lymphomas and Cases-Preceptor

Sept 29, 2004 Cutaneous Lymphomas and Cases

Oct 13, 2004 Hematology-Oncology Fellows Teaching Rounds- Immunohistochemistry: Applications

Oct 27, 2004 Pathology of Spleen and Cases

Nov 24, 2004 Pattterns of Lymph node Disorders and Cases

27
January 18,2005 WHO Classification of Lymphomas- B cell type

January 21, 2005 WHO Classification of Lymphomas- Peripheral T cell Lymphomas

July 19, 2005 Immunohistochemistry: Applications/ Theory/ Techniques in Histology

December 20, 2005 Hematopathology Resident Lecture Slides Series- Pathology of the Spleen

April 25, 2006 ASH paper Clinical Pathology presentation: New Technology of IHCFlow

May 16, 2006 Lymph node Reaction and Pattern of Benign Lymphadenopathies: Biology to Disease
Slide Series
June 13, 2006 Reactive lymph node- unknown slide workshop with residents and fellows

July 14, 2006 Advanced Immunohistochemistry Resident Lecture with Demos

Nov 21, 2006 Workshop on hematopathology diagnosis-competency based training on Potpourri of

Cases

Dec 20, 2006 Grand Round- B cell lymphomas

March 10, 2007 Teaching Rounds in Pathology- Splenic pathology and benign disorders

April 14, 2007 Grand Rounds- Cutaneous Lymphomas

July 24, 2007 Introduction to Histology and Special Immunostains II- Pathology Lecture

Nov 13, 2007 Lymphocytes from Biology to Disease Series- Reactive Lymph Nodes

Teaching Series

28
2005-2006 Hematopathology Lecture Series for Residents and Fellows-
Dec, 2005 to May, 2006
2007.2008 Hematopathology Fellow Teaching -Slide Weekly Slide Workshop Series
2007.2009 Hematopathology Fellow Teaching -Slide Weekly Slide Workshop Series

ACGME accredited Hematopathology Fellows

2006.2008 Dorna Rezania MD
2007.2008 Tina Yu MD
2008.2009 Elizabeth Sagatys MD
2009-current Mojdeh Naghashpour, MD

FELLOW SCHOLARLY ACTIVITY

A. List the articles authored or co-authored by fellows in your

program

Name of Fellow, Author(s) (Fellow Name in bold), Name of

Graduation year article, Name of journal, Volume, pages,
( Current Fellow, clinical) month Year
M. Naghashpour, H. Cualing, M.
Mojdeh Naghashpour
MD,2010
Szabunio, M. Bui, “Hyaline-Vascular
Castleman Disease: A Rare Cause of a
Solitary Subcutaneous
M. Naghashpour and H.Soft Tissue
Cualing
Mojdeh Naghashpour “Splenomegaly with Sea-Blue
MD,2010 Histiocytosis, Dyslipidemia and
Nephropathy in a Patient with Lecithin
Cholesterol Acyltransferase
Rebecca L. Olin, (LCAT)
Kim E. Nichols,
Mojdeh Naghashpour Mojdeh Naghashpour, Mariusz Wasik,
MD,2010 Brenda Shelly,Edward A. Stadtmauer,
and Dan T. Vogl1 “Successful use of
the anti-CD25 antibody daclizumab
Mojdeh Naghashpour and Lynn C. Moscinski,
Mojdeh Naghashpour
“Lymphoma”, in “Cancer Growth and
MD,2010
Progression, 2nd edition; Volume 2; Mechanisms
of Carcinogenesis”, Springer 2009.

Author(s) (Fellow Name in bold), Name of

Name of Fellow,
article, Name of journal, Volume, pages,
Graduation year
month Year

29
Elizabeth Sagatys MD Condarco T, Sagatys E, Prakash A,
2009 Rezania D, Cualing H. Primary
Cutaneous B-Cell Lymphoma in a Child.
Fetal and Pediatric Pathology.
Elizabeth Sagatys MD Sagatys E, Messina J, Rojiani A. 55-
2009 Year-Old Man with Paraspinal
Mass.Brain Pathology. 18(4):631-5.2008
Elizabeth Sagatys MD Saeed S, Sagatys E, Wasserman J,
2009 Morgan MB. Geriatric Fungal Infections.
In: Diagnosis of Aging Skin Diseases, RA
Norman (ed). pp 121-47.
Elizabeth Sagatys MD Sagatys E, Garrett C, Boulware D,
2009 Kelly S, Malafa M, Cheng J, Sebti S,
Coppola D. Activation of the
Serine/Threonine Protein Kinase Akt
Elizabeth Sagatys MD Saeed S, Sagatys E, Morgan MB.
2009 Acral Keratosis with Eosinophilic
Dermal Deposits: A Distinctive
Clinicopathologic Entity or Colloid
Yu, I-Ting (Tina), 2008 IT Yu, LC Moscinski, L Sokol, HD
Cualing. CD4+CD56+ hematodermic
neoplasm clinicopathologic study of
eight cases. AJSP (manuscript for

Author(s) (Fellow Name in bold), Name of

Name of Fellow,
Article, Name of Journal, Volume, pages,
Graduation year
Month Year
Dorna Rezania, 2007 Rezania D, Cualing HD, Ayala E. The diagnosis,
management, and role of hematopoietic stem cell
transplantation in aggressive peripheral T-cell
neoplasms. Cancer Control. 2007 Apr; 14 (2).
Dorna Rezania, 2007 Rezania D, Sokol L, Cualing H. Classification
and treatment of rare and aggressive types of
peripheral T-cell/natural killer-cell lymphomas
of the skin. Cancer Control. 2007 Apr; 14 (2).
Dorna Rezania, 2007 Rezania D, Ouban A, Marcet J, Coppola D.
CMV colitis mimicking recurrent inflammatory
bowel disease: report of three cases. Am Surg.
Dorna Rezania, 2007 2007
Todd J.Jan; 73 (1).
Alekshun, MD, Dorna Rezania, MD, Hernani Cualing,
MD, Ernesto Ayala, MD, Lubomir Sokol, MD. Skeletal Muscle
Peripheral T-Cell Lymphoma,
Journal of Clinical Oncology,Jan 20 ;26:501-3,2008

Dorna Rezania, 2007 Tania Condarco, MD, Elizabeth Sagatys, MD,

Dorna Rezania, MD, Amy Prakash, MD, Hernani
Cualing, MD. Primary Cutaneous B-cell Lymphoma in a
Child. Pediatric Dermatology,Fetal and Pediatric
Pathology, 2008 Dec 27:206-14

30
 Dorna Rezania, 2007 Erica Smithberger MD, Rezania Dorna MD,
Chavan R, Lein MH, Cualing H, Glass F, Messina JL.
Primary Cutaneous Angioimmunoblastic T-cell
lymphoma Histologically
Mimicking an Inflammatory Dermatosis. Submitted
Archives of Dermatology

B. List the research presentations by fellows (during the last three years) that
resulted from fellow research activity during the program.

Presenter(s) (Fellow Name in bold), Topic,

Name of Fellow,
Audience (or Name of Meeting), Location,
Graduation year
Date
Mojdeh Naghashpour, EM Sagatys, D Rezania, M
MD Naghashpour, LF Glass, ME Kadin and
HD Cualing “CLA, TRAF1, BCL2,
NFKB2, CD56, EMA and Fascin
Mojdeh Naghashpour, Naghashpour M, Sagatys E, Smith R,
MD Cualing H. “AIDS-Associated
Hemophagocytic Syndrome: A Study
of 100 Autopsy Cases” Oral Platform
Mojdeh Naghashpour, Mojdeh Naghashpour, Adam Bagg.
MD “The complementary role of molecular
genetic and conventional cytogenetic
detection of diagnostically and
Mojdeh Naghashpour, M. Naghashpour, Ahmed S Bedeir, Jed
MD Di-Paola, Yuan Yao, Roger Day and AR
Sepulveda1. (United States and
Canadian Academy of Pathology 98th
Annual Meeting Stowell-Orbison
Elizabeth Sagatys, MD 2009
Poster SessionBoston,
CLA, TRAF1, BCL2, NFKB2, CD56,Massachusetts
EMA and Fascin
Expression in CD30+ Lymphoproliferative Disorders of the Skin
EM Sagatys, D Rezania, M Naghashpour, LF Glass, ME Kadin,
HD Cualing
United States and Canadian Academy of
Pathology 98th Annual Meeting Stowell-
Orbison Poster Session
Elizabeth Sagatys, MD Boston, Massachusetts
Elizabeth Sagatys MD, 2009
Dorna Rezania MD,
2009 MD, Marshall Kadin MD, Frank Glass MD,
Hernani Cualing MD. CLA and TRAF1 are useful
in differentiating lymphomatoid papulosis,
cutaneous ALCL, and Systemic ALCL. Am
Society of Hematology poster, 2008, San
Francisco.
Elizabeth Sagatys, MD AIDS-Associated Hemophagocytic Syndrome: A
2009 Study of 100 Autopsy Cases M Naghashpour, EM
Sagatys, R Smith, HD Cualing
United States and Canadian Academy
of Pathology 98th Annual Meeting
Proffered Paper - Infections Session
Boston, Massachusetts, 2009

31
 Elizabeth Sagatys, MD Analysis of T-cell Subpopulations in
2009 the Lymph Nodes of Patients with
Mantle Cell LymphomaEM Sagatys,
L Moscinski, S Dessureault, E
Sotomayor, H Cualing American
Elizabeth Sagatys, MD Automated Determination of Bone
2009 Marrow Cellularity
EM Sagatys, H Cualing, L Kang, E
Zhong, L Moscinski
Yu, I-Ting (Tina),2008 IT Yu, D Rezania, LC Moscinski, JD
Messina, HD Cualing. Differentiating
Cd4+/Cd56+ Hematodermic
Neoplasm From Acute Monoblastic
Leukemia. United States and Canadian

Name of Fellow, Presenter(s) (Fellow Name in bold), Topic,

Graduation year Audience (or Name of Meeting), Location, Date

Dorna Rezania, 2007 Dorna Rezania, MD, Hernani D. Cualing, MD,

Eric Zhong MS, Lihua Li PhD. Conversion of Tissue
Chromogenic Staining to Virtual Flow Cytometry
by iHCFLOW System, United States and Canadian
Academy of Pathology (USCAP), annual meeting,
SanDiego, CA, 03/2007
Dorna Rezania, 2007 Ghayouri, M, Rezania, D, I-Ting YU , Moscinski, L, Zhong, E.
Cualing, H. Automated grading of the reticulin stain of bone
marrows using AUTORETIC algorithm. Accepted for United
States and Canadian Academy of Pathology (USCAP), annual
meeting, Denver, CO, 03/2008

Dorna Rezania, 2007 I-Ting Yu, MD, Dorna Rezania, MD, Lynn C.
Moscinski, MD, Jane D. Messina, MD, Hernani D.
Cualing, MD. Differentiating CD4+CD56+
Hematodermic neoplasms from acute monoblastic
leukemia. Accepted for United States and
Canadian Academy of Pathology (USCAP), annual
Dorna Rezania, 2007 meeting,
Elizabeth Denver
SagatysCO, 03/2008
Dorna Rezania MD, MD,
Marshall Kadin MD, Frank Glass MD, Hernani
Cualing MD. CLA and TRAF1 are useful in
differentiating lymphomatoid papulosis, cutaneous
ALCL, and Systemic ALCL. Am Society of
Hematology, 2008, San Francisco.

Research Support-Clinical Trials:

32
Title: A Phase II Trial Using a Universal GM-CSF-Producing and CD40L-Expressing Bystander Cell Line
(GM.CD40L) in the Formulation of Autologous Tumor Cell-Based Vaccines for Patients with Mantle Cell
Lymphoma.
Account : MCC# 13840
Type: Foundation Grant
Dates: 2005-2008
Source: Lymphoma Foundation of America
Role in the Study: Hematopathologist-Tissue Quantification of tumor infiltrating lymphocytes in mantle
cell lymphoma.

Title: Bortezomib and Vorinostat Trial in Refractory Mantle Cell Lymphoma

Account #: PI/Dr. Sokol Co Investigator: Hernani Cualing MD
Dates: 2008-2009
Source: H. Lee Moffitt Cancer Center
Role in the Study: Hematopathologist and quantification of target antigen

Title: “Medimmune anti CD2 targeted therapy in T cell lymphoma”

Account #: 14120 PI/Dr. Sokol Co Investigator, Hernani Cualing MD
Dates: 2006-2008
Source: H. Lee Moffitt Cancer Center
Role in the Study: Hematopathologist and quantifying target antigen

Title: “Single Agent Phase II study of Forodesine in the treatment of (CTCL) cutaneous T cell
lymphoma”
Account #: 14120 PI/Dr. Sokol Pathologist, Hernani Cualing MD
Dates: 2006-2008
Source: H. Lee Moffitt Cancer Center
Role in the Study: Hematopathologist and quantifying target antigen

Title: “Cytogenetic Studies in Leukemia Patients”

Account #: 14766 PI/Dr Lynn Moscinski, MD Collaborator/Hernani Cualing MD
Dates: 2007-2008

33
Source: H. Lee Moffitt Cancer Center

Account# 14794 PI/Lynn Moscinski, MD Collaborator/Hernani Cualing, MD

Date: 2007-2008
Source: H. Lee Moffitt Cancer Center
Title: Leukemia Centralized Reference Laboratories and Tissue Respositories, Ancillary

External Grant:
1. Account: RC1 Application PI/George Blanck, Ph.D. Co Investigator/Hernani Cualing, MD
Source: National Institute of Health
Title: “Mouse modeling of a CD30+ lymphoproliferative disorder: Role of IRF-1 defects in ALCLs”
% Effort 10%
Role in Study: Co PI: Hematopathologist comparing human CD30+ lymphomas with mouse CD30+
lymphomas
Date Submitted: June 14 , 2009- Not funded, Resubmission SBIR

2. New Multiinstitutional Grant submitted April 27, 2009: Exploratory/Developmental Research Grant
(R21) via American Recovery & Reinvestment Act of 2009 as RFA to Translational Science and
specific Challenge Topic 15-CA-105, The Biology of Cancer in Adolescents and Young Adults.:
using tissue blocks from SEER tissue banks from Hawaii, Iowa, RI, USC
Samples consist of diagnostic tumors from 527 Hodgkin lymphoma patients in the National
Cancer Institute Surveillance, Epidemiology and End-Results (SEER) program
Residual Tissue Repository (RTR). In Los Angeles, Hawaii and Iowa.
% effort: 3 %
Role: To provide hematopathology support and know-how for automated virtual flow analysis of T cell
subsets immunostains on these blocks.

COMPLETED
Internal Grant:
PI/Dr. R. Kothari Co PI Investigator.Hernani Cualing MD
Dates: 1994-1995

34
Source: University of Cincinnati/College of Engineering Biomedical Engineering Grant
Title: “Intelligent image analysis of lymphomas using artifical neural networks
% Effort: 10%
Role in the Study: Hematopathologist performing diagnosis and image analysis
Total Amount of Award: $ 24,000

Internal Grant:
PI: Director of Project Hernani Cualing MD
Dates: 1998-2000
Source: Dr. R Smith Discretionary Fund, Professor Univ of Cincinnati
Title: Color image segmentation of lymphocytes: Biomedical Engineering Grant
% Effort: 5 %
Role in the Study: Hematopathologist and quantification of target antigen

External Grant:
PI/Dr. R. Kothari Co Investigator.Hernani Cualing MD
Dates: 1997-2000
Source: Whitaker Foundation Biomedical Engineering Grant
Title: Microscopic Diagnosis of Lymphoma and Leukemia
% Effort: 15 %
Role in the Study: Hematopathologist performing diagnosis and image analysis
Total Amount of Award: $120,000

PATENTS and TRADEMARKS

1. USPTO/Virtual flow cytometry on immunostained tissue-Tissue cytometer
2. USPTO/ International PCT :Automated Cytomics: Bone Marrow Cellularity
Determination
3. USPTO/ Automated Cytomics: Bone Marrow Reticulin Fibrosis Determination
4. USPTO Registered Trademark: VirtualFlow IHCFLOW® Cytometer

PEER REVIEWED JOURNAL REVIEWER:

35
 Cytometry part B
Communications in Clinical Cytometry
Human Pathology
International Journal of Cancer
Transaction on Knowledge and Data Engineering
Leukemia Lymphoma
Pediatric Hematology
Pediatric Dermatology
Computer Methods and Programs in Biomedicine

Peer reviewed publications:

1) Bernal S, Cualing H, Elias A., Schwarting G and

McCluer R. Classification of Small Cell Carcinoma
Antigens by their Association with Subcellular
Components. Lung Cancer Journal 4: 99-102 1988.

2) Bernal S, Cualing H., Elias A and Siegel R. Stability and

Shedding of Small Cell Carcinoma Membrane
Antigens: Applications to Detection of Antigens in
Marrow and Serum. Lung Cancer Journal. 4: 79-80 1988.

3) Pliskin A, Cualing H, Stenger RJ. Primary Squamous Cell

Carcinoma of the Liver Originating in a Congenital Cyst
of the Liver. A Case Report and Review of Literature.
Arch of Path Lab Med. 116:105-107, Jan 1992.

36
4) Cualing H, Siegel R, Schwarting G, Suchy S, McCluer R
and Bernal,S. Expression of H-like blood group glycolipids
in Small Cell Carcinoma of the Lung. Hybridoma,
12(3):239-247, Jun 1993.*

5) Orazi A, Neiman R, Cualing H, Heerema N, John K. , CD34

Immunostaining of Bone marrow biopsy specimens is a reliable
way to classify the phases of chronic myeloid leukemia. Am J
Clin Pathol, 101(4):426-428, Apr 1994.

6) Kothari R, Cualing H. , Balachander T. Neural Network

Analysis of Flow Cytometric Immunophenotype Data.
IEEE Transactions in Biomedical Engineering, 43(8):803-
810,Aug 1996.

7) Cualing H, Povoski S, Fenoglio-Preiser C, Yassin R, Bell R.

Characterization of Hematologic malignancies in Syrian Golden
Hamsters, Carcinogenesis 17(9):1983-1988,1996.*

8) Balachander T, Kothari R, Cualing H. An empirical comparison

of dimensionality reduction techniques for Pattern Classification.
Gerstner W et al eds. Artificial Neural Networks ICANN ’97,
Lausanne, Switzerland. Lecture Notes in Computer Science1327:
589-594, 1997.

9) Cualing H, Vasireddy R. Adult T-cell leukemia and Cutaneous

pathology: An overview. J Geriat Dermatol 5(8): 377-382,
1997.*

37
10) Giza E*, Stern P, Cualing H. Aggressive Giant Cell Reparative
Granuloma of the Metacarpal. J Hand Surg AM 22A (4): 732-
736, 1997.

11) Massela F, Kowal-Vern A, Shrit A, Wibowo A, Rector J,

Cotelingham J, Collier J, Michael A, Cualing H,
Schumacher H. Acute Erythroleukemia. Am J Clin Pathol
110(5):590-598, Nov 1998.

12) Craig A*, Cualing H, Thomas G, Lamerson C, Smith R.

Cytophagic Histiocytic Panniculitis- a syndrome associated
with benign and malignant panniculitis: Case comparison and
review of the literature. J Amer Acad Dermatol 39(5):721-
736, part 1 Nov 1998.*

13) Cualing H, Balachander T, Kothari R. Immunophenotypic

diagnosis of acute leukemia using decision tree analysis.
Laboratory Investigation , 79(2): 205-212, Feb 1999.*

14) Nakanishi K, Cualing H, Husseinzadeh N. Dermatomyositis as

a presenting symptom of ovarian cancer. Obstetrics and
Gynecology 94(5): 836-838, part 2 Suppl S Nov 1999.

15) Marcelo A, Fontelo P, Farolan M, Cualing H. Effect of Image

Compression on Telepathology: A Randomized Controlled Trial.
Arch Pathol Lab Med, 124:1653-1656, Nov 2000.

16) Cualing H, Zellner D, Steele P. Blastic transformation of splenic

marginal zone B-cell lymphoma.. Arch Pathol Lab Medicine.
124(3):748-752 March 2000*

38
17) Cualing H. Perspectives: Automated Analysis in Flow
Cytometry.Communications in Clin Cytometry, 42:110-113,
April 2000.*

18) Cualing H. Wang G, Noffsinger A, Fenoglio-Preiser C.

Heterotopic ovarian splenoma:
report of a first case. Arch Pathol Lab Med. 2001
Nov;125(11):1483-5.

19) Cualing H, Schroder L Perme I. Allergic Granulomatosis

secondary to a Limited Churg-Strauss Lymphadenopathy: Case
Report with Histologic and Immunophenotypic analysis-
125:954-957, 2001 Arch of Pathol Lab Med July 2001

20) Gebrail F*, Knapp M, Perrotta G, Cualing H. Crystalline

Histiocytosis in Hereditary Cystinosis. 126:1135, Sept 2002,
Archives of Pathol Lab Med. (Direct supervision of medical
student elective in Diagnostic Hematopathology)

21) Kulow G, Breneman D, Cualing H, Steele P, Breneman J, Van

Horn J, Mutasim D. The Progression of Cutaneous B-cell
Pseudolymphoma (CBPL) to Cutaneous B-cell lymphoma
(CBCL). Vol 6(6), 519-528, Nov 2002, J of Cutaneous Med
and Surg.

22) Gebrail F*, Lowy A, Cualing H. Angiolymphoid hyperplasia

with eosinophilia: a pathologic quiz case. Vol 127, 113-114,
Jan 2003, Archives of Pathol Lab Med. (Direct
supervision of medical student elective in Diagnostic
Hematopathology)

39
23) Goldman A*, Bedolla G, Gebrail F, Cualing H, Bronchus-
Associated Lymphoid Tissue Lymphoma (BALT lymphoma).
Vol 127, 115-116, Jan 2003, Archives of Pathol and Lab Med.
(Direct supervision of medical student elective in Diagnostic
Hematopathology)

24) Breneman DL, Raju US, Breneman JC, Steele PE, McFadden
DW, Cualing HD, Nussbaum MS, Swerdlow SH.Lymph node
grading for staging of mycosis fungoides may benefit from
examination of multiple excised lymph nodes. J Am Acad
Dermatol. 2003 May;48(5):702-6.

25) Goldman A*, Gebrail F, Cualing H, Mott cell Granuloma: a

56 year old presenting with recurrent neck mass. Vol 127: June
2003 Archives of Pathology and Lab Medicine. (Direct
supervision of medical student elective in Diagnostic
Hematopathology)

26) Colvin JH, Lamerson CL, Cualing H, Mutasim DF. Cutaneous

lymphoplasmacytoid lymphoma (immunocytoma) with
Waldenstrom's macroglobulinemia mimicking rosacea.
J Am Acad Dermatol. 2003 Dec;49(6):1159-62.

27) Breneman DL, Raju US, Breneman JC, Steele PE, McFadden
DW, Cualing HD, Nussbaum MS, Swerdlow SH.Reply to
Letters to the Editor: Lymph node grading for staging of mycosis
fungoides may benefit from examination of multiple
excised lymph nodes. J Am Acad Dermatol. 2004
vol51:Sept 2004, 483-484.

40
28) Connie Keehn*, Cualing H., Marginal Zone Lymphoma with a
Dual Cutaneous and Leukemic Presentation and an Aberrant
MyeloidMonocytic Phenotype. Journal of Cutaneous Pathology
Volume 33: 6, 236-241, March 2006.

29) Rezania D*, Cualing H, Ayala E. Diagnosis, Management, And

The Role Of Hematopoietic Stem Cell Transplantation in
Aggressive Peripheral T Cell Neoplasms Cancer Control Journal,
vol 14, no.2, p 151, April 2007.

30) Rezania D*, Cualing H. Sokol L. Aggressive Peripheral T cell

Neoplasms and subtypes with high predilection in the Skin.
Cancer Control Journal, vol 14, no.2, p 116, April 2007.

31) Droc C*, Cualing H., Kadin M. The Need for an Improved
Classification For Therapy of CD30 + lymphomas. Cancer
Control Journal, vol 14, no.2, p 124, April 2007.

32) Takahashi Y, Coppola D, Matsushita N, Cualing HD, Sun M,

Sato Y, Liang C, Jung JU, Cheng JQ, Mulé JJ, Pledger WJ,
Wang HG. Bif-1 interacts with Beclin 1 through UVRAG and
regulates autophagy and tumorigenesis. Nature Cell Biol. 2007
Sep 23;

33) Khalil F*, Heekin J, Lili Miles, Cualing H. The rate and
morphometric pattern of bone marrow recovery post-
myelosuppressive therapy for acute leukemia: a quantitative
study- Archives of Pathology and Lab Medicine, Vol. 131, No.
8, pp. 1281–1289, Aug 2007

34) *Cualing HD,Moscinski LC, Zhong E. “Virtual Flow

Cytometry” of Immunostained Lymphocytes on Microscopic

41
 Tissue Slides, Clinical Cytometry part B, Volume 72B, Issue 1 , 
Pages 63 ­ 76 Jan 15, 2007.

35) Alekshun TJ, Rezania D, Ayala E, Cualing H, Sokol L.Skeletal

muscle peripheral T-cell lymphoma. J Clin Oncol. 2008 Jan
20;26(3):501-3. IF: 13.598 PMID: 18202425

36) J Pidala, Cualing H, Pinilla J. Acute basophilic leukemia arising

from chronic myelogenous leukemia with development of t(7;8)
(q34, q13) Cancer Genet Cytogenet. 2008 Apr 1;182 (1):46-9.

37) Raychaudhuri J, Cualing H, Haider K, Kharfan-Dabaja

MA.Granulocytic sarcoma and chronic lymphocytic leukemia of
the gastrointestinal tract after allogeneic hematopoietic cell
transplantation mimicking graft-versus-host disease. Leuk
Lymphoma. 2008 Feb;49(2):350-2. IF: 1.559 PMID: 18231925

38) Calder K, Khalil F, Schlauder S, Cualing H, Morgan M.

Immunohistochemical expression of survivin in cutaneous
sebaceous lesions. Am J Dermatopathol. 2008 Dec;30(6):545-
548. Pubmedid: 19033926.

39) Condarco T, Sagatys E, Prakash A, Rezania D, Cualing H.

Primary cutaneous B-cell lymphoma in a child. Fetal Pediatr
Pathol. 2008 Dec;27(4):206-214. Pubmedid: 18800263.

40) Bernal S, Speak J, Stahel R, Elias A, Mabry M, Cualing H.

Small cell carcinoma antigens. In: Rosen S, Mulshine J, Cuttita
F, Abrams P, eds. Biology in lung cancer: diagnosis and
treatment. New York: Marcel Dekker Inc., 1988:213-35: Book
Chapter

42
 41) Cualing H. Pathology of the Spleen. In Handbook of
Hematologic Pathology, Schumacher H, Stass S. Eds. 53-73,
Marcel Dekker, Inc., New York, April 2000.: Book Chapter

42) Mojdeh Naghashpour, Hernani Cualing* Splenomegaly with

sea-blue histiocytosis, dyslipidemia, and nephropathy in a patient
with lecithin-cholesterol acyltransferase deficiency: a
clinicopathologic correlation, in press 2009 Metabolism Clinical
and Experimental

43) Mojdeh Naghashpour, MD, PhD; Hernani D Cualing, MD;

Margaret Szabunio, MD;
Marilyn M Bui, MD, PhD Hyaline-Vascular Castleman Disease: A Rare Cause of Solitary
Subcutaneous Soft Tissue, in press, 2009 The American Journal of Dermatopathology
Manuscript Number: AJD-D-09-00143 *

* senior/corresponding author

Ongoing Research mentorship projects for fellows 2007 as topics in the monthly research
and planning meeting Hematopathology Mentorship:
Rezania D, Belongie I, Messina J, Cualing H, Glass F. Angioimmunoblastic T cell lymphoma
in skin- manuscript submitted for Archives of Dermatology

44) *Elizabeth Sagatys, MD, Lynn Moscinski, MD, Sophie

Dessureault, MD, Eduardo Sotomayor, MD and Hernani
Cualing, MD Analysis of T-cell Subpopulations in the Lymph
Nodes of Patients with Mantle Cell Lymphoma. Submitted to
49th ASH Annual Meeting and Exposition, Atlanta GA, Dec 8-
11, 2007.

43
45) Iriana Belongie, Jane Messina, Frank Glass, Hernani Cualing.
An unusual lymphoma evolving from CD8+ epidermotropic 
CTCL, 44th Annual Meeting,October 18-21, 2007 The
American Society of Dermatopathology, Baltimore Maryland
USA.

46) *Dorna Rezania, MD, Elizabeth Sagatys, MD, Marshall E.

Kadin, MD, Frank Glass, MD, Hernani Cualing, MD. CLA and
TRAF-1 are Useful in Differentiating Lymphomatoid Papulosis,
Cutaneous ALCL and Systemic ALCL, H. Lee Moffitt Cancer
Center and Research Institute1, Department of Pathology, The
University of South Florida College of Medicine, Tampa, FL and
Roger Williams Medical Center, Providence RI. Poster to be
submitted to USCAP 2009

47) *Tina Yu, Cualing H, Sagatys E, Rezania D, Moscinski L.

Hematodermic tumor and monocytic sarcoma: Differential
immunostaining for USCAP meeting, presented in March 2008
IAP Denver Meeting.

48) *Sagatys, E., H Cualing, E Zhong and L Moscinski.

Automated Determination of Bone Marrow Cellularity presented
in IAP. For submission to Cytometry part B.

49) *Sagatys E, Cualing H, Rezania D, Naghashpour M, Shaheen

G, Moscinski L, CD71 (Transferrin Receptor) Expression in
Precursor and Mature B- and T-cell lymphomas Recognized by
REAL/WHO Classification

50) Cualing H, Sagatys E, Naghashpour M, Smith R.

Macrosplenomegaly In AIDS. Manuscript for Archives of
Pathology and Lab Medicine

44
51) * Yu T, Calder K, Moscinski L, Sokol L, Morgan M, Cualing H.
Differential expression of myelomonocytic Markers in
Hematodermic Neoplasm and Acute Monocytic Leukemia.

52) Niessen Melissa, Blanck George, Cualing Hernani. Thymic

dysmorphogenesis and dysfunction in head trauma patients using
molecular and immunohistochemical markers. Dept of Molecular
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