Experimental Design 4 2 1 2 1 1

Biomedical Sciences Experimental Design 4.2.
1
Lydia Phillis and Maddy MacLachlan
1. Identify the Problem or Question
How do different activities affect heart rate?
2. Predict a solution to the problem or an answer to the question.
If activity is increased then heart rate will also increase.
Independent Variable: Activity done to change heart rate
Dependent Variable: Heart rate
During this experiment the outcome will be that as activity increases so will heart rate.
The independent variable would be the activity done to change the heart rate as that variable is
the one being manipulated. The dependent variable is a direct result of the independent therefor
being the actual heart rate.
3. Design the experiment to be used to test your hypothesis.
Materials:
Computer with Vernier Logger Prosoftware
Vernier LabQuest Mini with USB cable
Vernier Heart Rate Hand-Grip Monitor
Logger Pro resource sheet
Project 4.2.1 Medical History resource sheet
Ice water bath
Towel
Timer or watch
Colored pencils or markers
Laboratory journal
PBS Course File
PLTW Biomedical Science Experimental Design resource sheet

Control: Resting Heart Rate
Procedure:
Seated comfortably, find your carotid pulse.Your partner should act as a timer as you count the
number of beats you feel in 30 seconds. Multiply this number by two to obtain your heart rate in
beats per minute (bpm). We refer to this value as your resting heart rate.
Switch roles and allow your partner to determine his/her heart rate.
Repeat this process until you have measured your resting heart rate three times. Record these
values in your laboratory journal and calculate your average resting heart rate.
Refer to the Logger ProResource Sheet as you are working with Logger Pro software and Vernier
probes.
Obtain a Vernier Hand Grip Heart Rate monitor. You will use this probe to determine your heart rate
and compare this value to the resting heart rate you measured in Part II.
Turn off any cell phones and disable the wireless connection on the computer. These devices can
interfere with the hand-grip heart rate monitor because it is wireless.
Start Logger Pro software on your computer.
Click on File Open and open the Human Physiology with Vernier folder.
Open the program titled 03 Heart Rate Vital Sign.
Connect the LabQuest Mini to the computer using the USB cable.
Connect the Handgrip Heart Rate Monitor into CH 1 of the LabQuest Mini using the British Telecom
connector.
Notice the Handgrip Heart Rate Monitor has two parts. There is a small rectangular box with an
arrow, and there are two connected cylinders. The small box has the British Telecom connector and
is connected to the LabQuest Mini. The two cylinders are the hand grips, and they have clearly
marked hand outlines to show proper hand placement. Both grips must be held by the person being
tested.
Notice the arrow on one of the hand grips and the arrow on the small black receiver. These arrows
must be aligned to use the probe. Have one partner hold the receiver next to the hand grip with the
arrows aligned when you begin collecting data. Once data appears on the front panel, you will be
able to move the receiver up to 2 feet away and still take measurements.
Adjust the length of time for the experiment. To obtain initial heart rate data, set the timer for 60
seconds. Click on the button in the toolbar, adjust the Length accordingly, and click Done.
Click the green Collect arrow in the top toolbar.
Notice it may take 15 to 20 seconds after you press the Collect arrow for data to appear on the data
collection graphs. After that time, you should see a waveform graph appear in the Heart Beats graph
and the calculated beats per minute appear on the Heart Rate as a Vital Sign graph. Note that the
table on the left hand side of the screen charts the recorded heart rate at 5 second intervals.
Click the red Stop bar when you are finished collecting data. Note that if you set the experiment to a
predetermined length, the data collection will stop automatically.
Save the data to a file as directed by your teacher.
Record in your laboratory journal the average heart rate in beats per minute. This value will appear
in the white box on the left side of the screen. Compare this value to the average heart rate you
measured in Part I. Discuss why these two values may differ.
Copy the Heart Rate as a Vital Sign graph by placing the mouse cursor over the graph, left clicking,
and then pressing both the Control and the C keys simultaneously. Paste the graph into a Word
document and save it. To paste the graph, press both the Control and the V keys simultaneously.
Repeat this step in order to copy and paste the Data Table into the Word document. Save your
printed data in your laboratory journal.
Perform your experiment as you designed it. Follow the instructions presented in Part III to guide
your use of the heart rate probe.
Collect data in your laboratory journal. Print graphs and charts from the computer as needed and
include these with your written work. Make sure to clearly explain your results
4. Carry out the experiment.

Data:
Resting Heart Rate
Trail 1
Trail 2
Trail 3
10 Second Heart
Rate (bpm)
80 bpm
85 bpm
72 bpm
30 Second Heart
Rate (bpm)
82 bpm
88 bpm
70 bpm
45 Second Heart
Rate (bpm)
80 bpm
85 bpm
74 bpm
60 Second Heart
Rate (bpm)
83 bpm
83 bpm
80 bpm
Heart Rate During Jumping Jacks

Trail 1
Trail 2
Trail 3
10 Second Heart
Rate (bpm)
91 bpm
121 bpm
93 bpm
30 Second Heart
Rate (bpm)
94 bpm
100 bpm
94 bpm
45 Second Heart
Rate (bpm)
88 bpm
88 bpm
84 bpm
60 Second Heart
Rate (bpm)
81 bpm
79 bpm
82 bpm
Heart Rate While Holding Breath

Trail 1
Trail 2
Trail 3
10 Second Heart
Rate (bpm)
84 bpm
91 bpm
86 bpm
30 Second Heart
Rate (bpm)
82 bpm
93 bpm
86 bpm
45 Second Heart
Rate (bpm)
88 bpm
97 bpm
102 bpm
60 Second Heart
Rate (bpm)
96 bpm
5. Analyze the data and observations.
98 bpm
105 bpm
6. State the conclusion.

The hypothesis was correct because as the amount of activity rose, the heart rate also went up
due to that activity.
7. Summary Paragraph
From seeing the beginning rates of the resting heart rate to the heart rates of those who
included activity, the change is adamant. While resting, the heart rates were at about 85-80
bpm, but while doing some sort of exercise the rate dramatically increased. The heart rate from
doing jumping jacks went up to 88-120 and holding your breath made the heart rate go from 82105. As the heart pumps, if you're resting it can go at a normal pace to produce the right amount
of blood to be carried through the body, but when you exercise you need more blood faster.
Therefor your heart has to work harder, meaning it beats more per minute to achieve the
amount you need to sustain a healthy level.

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Biomedical Sciences Experimental Design 4.2.

Computer with Vernier Logger Prosoftware

Vernier LabQuest Mini with USB cable

Vernier Heart Rate Hand-Grip Monitor

Logger Pro resource sheet

Project 4.2.1 Medical History resource sheet

Ice water bath

Colored pencils or markers

PBS Course File

PLTW Biomedical Science Experimental Design resource sheet

4. Carry out the experiment.

Heart Rate During Jumping Jacks

Heart Rate While Holding Breath

5. Analyze the data and observations.

6. State the conclusion.

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