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Scale-Up Examples and Problems

This document provides examples and problems related to scaling up fermentation processes from laboratory to production scale. It addresses topics like determining dimensions and operating conditions for scaled-up fermenters to maintain geometric similarity, estimating mixing times for different viscosities and agitator speeds, calculating the minimum agitator speed needed to ensure turbulent mixing, comparing power requirements at different viscosities, and determining maximum stirrer speeds and mixing times based on power constraints.

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Rooth Adajet
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814 views2 pages

Scale-Up Examples and Problems

This document provides examples and problems related to scaling up fermentation processes from laboratory to production scale. It addresses topics like determining dimensions and operating conditions for scaled-up fermenters to maintain geometric similarity, estimating mixing times for different viscosities and agitator speeds, calculating the minimum agitator speed needed to ensure turbulent mixing, comparing power requirements at different viscosities, and determining maximum stirrer speeds and mixing times based on power constraints.

Uploaded by

Rooth Adajet
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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Examples and problems:

1. Consider the scale-up of a fermenter producing E-coli from nominal volume of 10L to 10m3 with aspect
ratio of 3. Impeller diameter is 30% of tank diameter. Assume geometric similarity. If agitator speed is
500 rpm with 3 rushton-turbine impellers, determine dimensions of the large fermenter and agitator
speed for constant;
a. Po/VL
b. Tip speed
c. NRe
d. Decide which operating condition is best suitable for e-coli and for a CHO cell line
e. Compare mixing speed if lab-scale is changed to 1 impeller-system with 500 rpm vs. 3 impellersystem. Determine speed of a 3-impeller system.
2. A fermentation broth with viscosity 10-2 Pa.s and density 1000 kg/m3 is agitated in a 2.7m3 working
volume using rushton turbine impellers with diameter 0.5m and stirrer speed of 1 s-1.
a. Estimate mixing time.
b. If speed is increased by 30%, what is the new mixing time?
3. To ensure turbulent conditions and minimum mixing time during agitation with a turbine impeller, the
Reynolds number must be at least 104.
a. A stirred laboratory-scale fermenter with a turbine impeller 5 cm in diameter. If the density of
broth being stirred is close to that of water with 3.33cp, what is the minimum impeller speed
necessary if adequate mixing is to be maintained?
b. The mixing system is scaled up so the tank and impeller are 15 times the diameter of the
laboratory equipment. The stirrer in the large vessel is operated so that the stirrer tip speed (tip
speed = pi. N.Di) is the same as in the laboratory apparatus. How does scale-up affect the
maximum viscosity allowable for maintenance of turbulent mixing conditions?
4. A cylindrical bioreactor diameter 3m has 4 baffles. A rushthon turbine mounted in the vessel has a
diameter 1/3 of tank diameter and is operated at a speed of 90 rpm. The density of the fluid is similar to
water. The reactor is used to culture an anaerobic organism in which broth initially can be assumed
Newtonian but as cells grow, viscosity increases. Compare power requirements when viscosity is;
a. Equal to water
b. 100x greater than water
c. 104x greater than water
5. Laboratory-scale fermenters are usually mixed using small stirrer motors between 100-500W. Once such
motor is used to drive a 10-cm turbine impeller in a small reactor containing fluid with the properties of
water. The stirrer speed is 900rpm. Estimate power requirements for this process if the actual stirrerdrive is a 300-W motor.
a. How do you explain the difference between the amount of electrical power consumed by motor
and power delivered to the fluid?

6. A cylindrical stirred bioreactor with diameter and height of liquid to 2m has a rushton turbine 1/3 of tank
diameter. The fermentation broth is a Newtonian fluid with viscosity 4cp.
a. If the specific power consumption must not exceed 1.5kW/m3, determine the maximum
allowable stirrer speed. What is the mixing time under these conditions?

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