Agilent G1316 90011 TCC A B C Ebook
Agilent G1316 90011 TCC A B C Ebook
Agilent G1316 90011 TCC A B C Ebook
Thermostatted Column
Compartment
G1316A/G1316B/G1316C
User Manual
1200 Series TCC User Manual
Agilent Technologies
Notices
Agilent Technologies, Inc. 1995,
1996-2008
No part of this manual may be reproduced
in any form or by any means (including electronic storage and retrieval or translation
into a foreign language) without prior agreement and written consent from Agilent
Technologies, Inc. as governed by United
States and international copyright laws.
Edition
09/2008
Printed in Germany
Agilent Technologies
Hewlett-Packard-Strasse 8
76337 Waldbronn
Manual Structure
The User Manual G1316-90011 (English)
and its localized versions contain a subset
of the Service Manual and is shipped with
the Thermostatted Column Compartment in
printed matter.
Latest versions of the manuals can be
obtained from the Agilent web.
Warranty
The material contained in this document is provided as is, and is subject to being changed, without notice,
in future editions. Further, to the maximum extent permitted by applicable
law, Agilent disclaims all warranties,
either express or implied, with regard
to this manual and any information
contained herein, including but not
limited to the implied warranties of
merchantability and fitness for a particular purpose. Agilent shall not be
liable for errors or for incidental or
consequential damages in connection
with the furnishing, use, or performance of this document or of any
information contained herein. Should
Agilent and the user have a separate
written agreement with warranty
terms covering the material in this
document that conflict with these
terms, the warranty terms in the separate agreement shall control.
Safety Notices
CAUTION
A CAUTION notice denotes a
hazard. It calls attention to an
operating procedure, practice, or
the like that, if not correctly performed or adhered to, could
result in damage to the product
or loss of important data. Do not
proceed beyond a CAUTION
notice until the indicated conditions are fully understood and
met.
Technology Licenses
The hardware and/or software described in
this document are furnished under a license
and may be used or copied only in accordance with the terms of such license.
WA R N I N G
A WARNING notice denotes a
hazard. It calls attention to an
operating procedure, practice,
or the like that, if not correctly
performed or adhered to, could
result in personal injury or
death. Do not proceed beyond a
WARNING notice until the indicated conditions are fully understood and met.
In This Guide
In This Guide
6 Test Functions
This chapter describes the TCCs built in test functions.
In This Guide
7 Maintenance
This chapter describes the maintenance of the TCC.
9 Appendix
This chapter provides addition information on safety, legal and web.
Contents
Contents
Main Features 10
System Overview 11
Column-Identification System 13
Column Switching Valve (Optional for G1316A/G1316B SL)
Electrical Connections 18
Instrument Layout 21
Agilent Diagnostic Software 22
2 Site Requirements and Specifications
23
15
30
33
51
52
Contents
55
61
56
66
71
79
91
Contents
9 Appendix
113
117
Contents
1
Introduction to the Column
Compartment
Main Features
10
System Overview
11
Column-Identification System
13
15
20
21
22
Agilent Technologies
Main Features
10
System Overview
11
IZbeZgVijg8
=ZViZmX]Vc\ZgiZbeZgVijgZ
8dajbciZbeZgVijgZ
:mVbeaZ[dgV[adlgViZd[*ba$b^cd[lViZg
I^bZb^c
Figure 1
12
Column-Identification System
6ciZccVh
8dajbc"^YZci^[^XVi^dciV\
8dajbcXa^e
Figure 2
Column-Identification System
Table 1 on page 13 shows the information that can be stored. The information
fields can be edited via the user interface.
Table 1
Item
Example
Product number
79916OD-552
Serial number
950522
Batch number
1675
Geometry [mm]
100 2.1
Stationary phase
ODS Hypersil
Particle size
10 m
Number of injections
1267
400
Comment
Date of manufacturing
13
Item
Example
70
Maximum pH recommended
12
Comment
The number of injections will be updated each run to create a column lifecycle
(history). The user interface allows to edit all information.
NOTE
14
If a column switching valve (see Column Switching Valve (Optional for G1316A/G1316B
SL) on page 15) is installed in the module, the update of the number of injections depends
on the position of the column switching valve. For example, if the left column is selected,
the right column is not updated, and vice versa. If no column switching valve is installed
both sides are updated at the same time.
Figure 3
15
NOTE
Before switching the valve, switch off the pump or set the flow to zero. Keeping the flow on
while the valve is switched can cause exceeding the maximum pressure. This will stop
method or sequence execution.
;gdbVjidhVbeaZg
=ZViZgVhhZbWan&
=ZViZgVhhZbWan'
8dajbc&
8dajbc'
IdYZiZXidg
Figure 4
Column 1 Active
;gdbVjidhVbeaZg
=ZViZgVhhZbWan'
=ZViZgVhhZbWan&
8dajbc'
8dajbc&
IdYZiZXidg
Figure 5
16
Column 2 Active
Precolumn Back-flushing
The sample is injected into series-connected precolumn and analytical
column. After the valve has switched, the analytical column flow continues in
normal direction. Only the precolumn is back-flushed, eluting highly retained
peaks directly to the detector.
;gdbVjidhVbeaZg
=ZViZgVhhZbWan'
=ZViZgVhhZbWan&
EgZXdajbc
6cVani^XVa
8dajbc
IdYZiZXidg
Figure 6
Precolumn Back-flushing
17
Electrical Connections
The CAN bus is a serial bus with high speed data transfer. The two
connectors for the CAN bus are used for internal Agilent 1200 Series
module data transfer and synchronization.
One analog output provides signals for integrators or data handling
systems.
The REMOTE connector may be used in combination with other analytical
instruments from Agilent Technologies if you want to use features such as
start, stop, common shut down, prepare, and so on.
With the appropriate software, the RS-232C connector may be used to
control the module from a computer through a RS-232C connection. This
connector is activated and can be configured with the configuration switch.
See your software documentation for further information.
The power input socket accepts a line voltage of 100 240 volts AC 10%
with a line frequency of 50 or 60 Hz. Maximum power consumption is
220 VA. There is no voltage selector on your module because the power
supply has wide-ranging capability. There are no externally accessible
fuses, because automatic electronic fuses are implemented in the power
supply. The security lever at the power input socket prevents the module
cover from being taken off when line power is still connected.
NOTE
18
Never use cables other than the ones supplied by Agilent Technologies to ensure proper
functionality and compliance with safety or EMC regulations.
HZXjg^inaZkZg
6E<GZbdiZ
GH"'('8
86C
8dc[^\jgVi^dchl^iX]
EdlZg
hV[ZinhiVcYVgYh
Xdc[^\jgVi^dchl^iX]
hZii^c\h
kdaiV\ZgVc\Z
edlZgXdchjbei^dc$
[gZfjZcXn
Figure 7
19
20
CCYWWSSSSS
Format
CC
country of manufacturing
DE = Germany
JP = Japan
CN = China
YWW
SSSSS
Instrument Layout
21
The Agilent Diagnostic Software is a standalone product that can be used with
or without data system. Agilent Lab Advisor helps to manage the lab for high
quality chromatographic results and can monitor in real time a single Agilent
LC or all the Agilent GCs and LCs configured on the lab intranet.
Agilent Lab Advisor provides diagnostic capabilities for all Agilent 1200 Series
HPLC modules. This includes tests and calibrations procedures as well as the
different injector steps to perform all the maintenance routines.
Agilent Lab Advisor also allows users to monitor the status of their LC
instruments. The Early Maintenance Feedback (EMF) feature helps to carry
out preventive maintenance. In addition, users can generate a status report for
each individual LC instrument. The tests and diagnostic features as provided
by the Agilent Lab Advisor Software may differ from the descriptions in this
manual. For details refer to the Agilent Lab Advisor help files.
This manual provides lists with the names of Error Messages, Not Ready
messages, and other common issues.
22
2
Site Requirements and Specifications
Site Requirements and Specifications
Power Consideration 24
Power Cords 25
Bench Space 26
Environment 26
Physical Specifications
24
27
Performance Specifications
28
Agilent Technologies
30
23
Power Consideration
The module power supply has wideranging capability (see Table 2 on page 27).
It accepts any line voltage in the range described in the above mentioned table.
Consequently there is no voltage selector in the rear of the module. There are
also no externally accessible fuses, because automatic electronic fuses are
implemented in the power supply.
WA R N I N G
WA R N I N G
Module is partially energized when switched off, as long as the power cord is
plugged in.
Repair work at the module can lead to personal injuries, e.g. shock hazard, when the
cover is opened and the module is connected to power.
Remove the power cable from the instrument before opening the cover.
Do not connect the power cable to the Instrument while the covers are removed.
24
CAUTION
unplugged.
Provide sufficient space behind the power socket of the instrument to unplug the
cable.
Power Cords
Different power cords are offered as options with the module. The female end
of all power cords is identical. It plugs into the power-input socket at the rear
of the module. The male end of each power cord is different and designed to
match the wall socket of a particular country or region.
WA R N I N G
The absence of ground connection and the use of an unspecified power cord can
lead to electric shock or short circuit.
Electric Shock
Never operate your instrumentation from a power outlet that has no ground
connection.
Never use a power cord other than the Agilent Technologies power cord designed
WA R N I N G
25
Bench Space
The column compartment dimensions and weight (see Physical
Specifications on page 27 ) allow to place this module on almost any desk or
laboratory bench. It needs an additional 2.5 cm (1.0 inches) of space on either
side and approximately 8 cm (3.1 inches) in the rear for the circulation of air
and electric connections.
If the bench should carry a complete Agilent Series system, make sure that
the bench is designed to carry the weight of all the modules.
The module should be operated in a horizontal position.
Environment
Your column compartment will work within specifications at ambient
temperatures and relative humidity as described in Physical
Specifications on page 27.
26
Physical Specifications
Table 2
Physical Specifications
Type
Specification
Weight
Dimensions
(width depth height)
Line voltage
Line frequency
50 or 60 Hz, 5%
Power consumption
Ambient operating
temperature
0 55 C (32 131 F)
Ambient non-operating
temperature
-4070 C (-4158 F)
Humidity
Operating Altitude
Non-operating altitude
Comments
Wide-ranging capability
Maximum
Non-condensing
27
Performance Specifications
Table 3
28
Type
Specification
Comments
Temperature
range
G1316A
G1316B (SL)/G1316C (SL Plus)
Temperature
stability
0.15 C
0.05 C
G1316A
G1316B (SL)/G1316C (SL Plus)
Temperature
accuracy
0.8 C
0.5 C
With calibration
Column
capacity
Three 30 cm
Warm-up/co
ol-down time
Dead volume
Communicati
ons
Safety and
maintenance
NOTE
Type
Specification
GLP features
Housing
Comments
All specifications are valid for distilled water at ambient temperature (25 C), set point at
40 C and a flow range from 0.25 ml/min.
29
A"h]VeZYegZ"Xdajbc
]ZViZg=ZViZg
adc\"Ydlc
A"h]VeZYegZ"Xdajbc]ZViZg
KdajbZ/&#+a
bdjciZYdcXVgg^Zg
ide[dgXdajbc&!
Wdiidb[dgXdajbc'
Figure 8
J"h]VeZYedhi"XdajbcXddaZg
KdajbZ/&#*a
bdjciZYdcXVgg^Zg
30
NOTE
If the additional heater and cooling devices are used as shown in Figure 8 on page 30, the
column identification system cannot be used. If the column identification system is
required, fix the heater and cooling devices in the upper or lower locations or fix them
right/left of the current location.
31
32
3
Installing the Column Compartment
Unpacking the Column Compartment
Delivery Checklist 34
Optimizing the Stack Configuration
Installing the Column Compartment
34
35
38
42
44
47
49
Agilent Technologies
33
If the delivery packaging shows signs of external damage, please call your
Agilent Technologies sales and service office immediately. Inform your service
representative that the module may have been damaged during shipment.
CAUTION
Delivery Checklist
Ensure all parts and materials have been delivered with the module. The
delivery checklist is shown below. Please report missing or damaged parts to
your local Agilent Technologies sales and service office.
Table 4
34
Description
Quantity
Power cable
CAN cable
optional
User Manual
If your column compartment is part of a Agilent 1200 Series system, you can
ensure optimum performance by installing the following configuration. This
configuration optimizes the system flow path and ensures minimum delay
volume.
For installations of the G1316C SL Plus as part of the Method Development
Solution, please refer to the Method Development Solution User and
Installation Guide part number: G4230-90000.
35
HdakZciXVW^cZi
KVXjjbYZ\VhhZg
Ejbe
AdXVa
JhZg>ciZg[VXZ
6jidhVbeaZg
8dajbcXdbeVgibZci
9ZiZXidg
Figure 9
36
68edlZg
GZbdiZXVWaZ
86C7jhXVWaZid
adXVajhZg^ciZg[VXZ
86C7jhXVWaZ
A6CidA8
8]ZbHiVi^dcadXVi^dc
YZeZcYhdcYZiZXidg
6cVad\YZiZXidg
h^\cVa
&dg'djiejiheZg
YZiZXidg
Figure 10
37
Parts required
Description
Column compartment
Power cord
For other cables see text below
Preparations
WA R N I N G
Module is partially energized when switched off, as long as the power cord is
plugged in.
Risk of stroke and other personal injury. Repair work at the module can lead to
personal injuries, e. g. shock hazard, when the module cover is opened and the
instrument is connected to power.
Never perform any adjustment, maintenance or repair of the module with the top
off when line power is still connected. Never plug the power line back in when cover
is removed.
CAUTION
Valve properties are read from the valve head RFID tag during initialization of the
module. Valve properties will not be updated, if the valve head is replaced while the
module is on.
Selection of valve port positions can fail, if the instrument does not know the
properties of the installed valve.
Always switch off the instrument when replacing the valve head.
38
CAUTION
The valve actuator contains sensitive optical parts, which need to be protected from
dust and other pollutions. Pollution of these parts can impair the accurate selection of
valve ports and therefore bias measurement results.
Always install a valve head for operation and storage. For protecting the actuator, a
dummy valve head (part of transportation lock kit part number: G1316-67001) can be
used instead of a functional valve. Do not touch parts inside the actuator.
2 Remove the dummy valve head by unscrewing the cap nut and removing it
from the valve drive (G1316C SL Plus only).
39
A^cZedlZghl^iX]
l^i]\gZZca^\]i
Figure 11
5 Connect the power cable to the power connector at the rear of the column
compartment.
6 Connect the CAN cable to other Agilent 1200 Series modules.
7 If Agilent ChemStation is the controller, connect either
40
NOTE
8 Connect the APG Remote cable (optional) for non-Agilent 1200 Series
instruments.
9 Turn ON power by pushing the button at the lower left side of the column
compartment. The status LED should be green.
HZXjg^inaZkZg
8dc[^\jgVi^dchl^iX]
86C
6E<GZbdiZ
GH"'('8
Figure 12
NOTE
EdlZg
The column compartment is turned on when the line power switch is pressed and the green
indicator lamp is illuminated. The column compartment is turned off when the line power
switch is protruding and the green light is OFF.
41
Several optional valve heads are available for the G1316C SL Plus, which can
be installed and exchanged easily.
Parts required
CAUTION
Part number
Description
5067-4107
5067-4108
Valve Damage
Using a low pressure valve on the high pressure side can damage the valve.
When using multiple column compartments as part of a method development
solution, make sure that the high pressure valve head is connected to the
autosampler and the low pressure valve head is connected to the detector.
For details, please refer to the Method Development Solution User and Installation
CAUTION
42
1 Put the valve head onto the valve drive such that the lobe 2 Screw the valve head onto the valve drive using the union
fits to the groove.
nut.
3 Install all required capillary connections to the valve head. 4 Push the valve head until it snaps in and stays in the rear
position.
43
Parts required
Description
Other modules
Parts from accessory kit, see Accessory Kits on page 102
Two wrenches 1/4 5/16 inch for capillary connections
Preparations
WA R N I N G
goggles, safety gloves and protective clothing) as described in the material handling
and safety data sheet supplied by the solvent vendor, especially when toxic or
hazardous solvents are used.
6ciZccVh
8dajbciV\
8dajbcXa^e
44
NOTE
NOTE
8a^e
IdYZiZXidg
NOTE
5 Fix the column with the column clip from the accessory
kit.
45
NOTE
46
Always operated the TCC with the front cover in place for proper thermostatting conditions
and to protect the column area against strong drafts from the ouside.
NOTE
Depending on the application, these heater and cooling devices can be fixed at various
places. Information about the usage of these heater and cooling devices can be found in
Technical Notes or in the Agilent 1200 Series Rapid Resolution LC System manual (part
number: G1312-90300).
]daZhidViiVX]XVgg^Zg
A"h]VeZYegZ"Xdajbc]ZViZg
KdajbZ/&#+a
bdjciZYdcXVgg^Zg
ide[dgXdajbc&!
Wdiidb[dgXdajbc'
Figure 13
NOTE
J"h]VeZYedhi"XdajbcXddaZg
KdajbZ/&#*a
bdjciZYdcXVgg^Zg
If the additional heat exchanger and cooling devices are used as shown in this figure, the
column identification system cannot be used. If the column identification system is
required, fix the heater and cooling devices in the upper or lower locations or right/left of
the current location.
47
7j^aY"^c]ZViZmX]Vc\Zg
Figure 14
48
Placing Columns
Column-Identification Tag
When correctly placed on the heat exchanger, the distance between the
column-identification tag and antenna is 12 mm. This is the optimum
distance for proper function. The identification tag can be easily removed from
the column.
NOTE
For columns with small diameter, a cable tie wrap should be used to fix the column
identification tag to the column. Assure that the tie wrap does not block the front cover.
NOTE
The tag needs to be placed differently, depending on whether the column is installed at the
left or right heat exchanger, see Figure 15 on page 49 and Figure 16 on page 50. The Agilent
logo should always be at front.
8dajbc"^YZci^[^XVi^dciV\
Figure 15
49
8dajbc"^YZci^[^XVi^dciV\
Figure 16
Column Clip
For better positioning of the column on the heat exchanger a column clip is
available (see Accessory Kits on page 102).
Figure 17
50
4
How to optimize the Column
Compartment
Optimizing the Performance of your Column Compartment
Using Additional Heater and Cooling Devices
52
53
Agilent Technologies
51
52
53
54
5
Troubleshooting and Diagnostics
Overview of the Column Departments Indicators and Test Functions
56
Status Indicators 57
Power Supply Indicator 57
Module Status Indicator 57
Available Tests depending on User Interfaces
Agilent Diagnostic Software
59
60
Agilent Technologies
55
Status Indicators
The instrument is provided with two status indicators which indicate the
operational state (prerun, run, and error states) of the instrument. The status
indicators provide a quick visual check of the operation of the instrument.
Error Messages
In the event of an electronic, mechanical or hydraulic failure, the instrument
generates an error message in the user interface. The following pages describe
the meaning of the error messages. For each message, a short description of
the failure, a list of probable causes, and a list of suggested actions to fix the
problem are provided.
56
Status Indicators
Two status indicators are located on the front of the module. The lower left
indicates the power supply status, the upper right indicates the instrument
status.
HiVijh^cY^XVidg
\gZZc$nZaadl$gZY
A^cZedlZghl^iX]
l^i]\gZZca^\]i
Figure 18
57
58
NOTE
Depending on the used interface, the available tests and the screens/reports may vary.
Preferred tool should be the Agilent Lab Advisor Software, see Agilent Diagnostic
Software on page 22.
Screenshots used in this document are based on the Agilent ChemStation. In future, the
user interface may not show the Diagnostics/Tests anymore. In this case use the Agilent
Lab Monitor Diagnostic (LMD) Software.
The Agilent ChemStation may not include any maintenance/test functions.
Table 5
Test
Agilent ChemStation
LMD Software
Yes
No
Yes
Temperature Calibration
Yes
Yes 1
Yes
section Maintenance
59
The Agilent Diagnostic Software is a standalone product that can be used with
or without data system. Agilent Lab Advisor helps to manage the lab for high
quality chromatographic results and can monitor in real time a single Agilent
LC or all the Agilent GCs and LCs configured on the lab intranet.
Agilent Lab Advisor provides diagnostic capabilities for all Agilent 1200 Series
HPLC modules. This includes tests and calibrations procedures as well as the
different injector steps to perform all the maintenance routines.
Agilent Lab Advisor also allows users to monitor the status of their LC
instruments. The Early Maintenance Feedback (EMF) feature helps to carry
out preventive maintenance. In addition, users can generate a status report for
each individual LC instrument. The tests and diagnostic features as provided
by the Agilent Lab Advisor Software may differ from the descriptions in this
manual. For details refer to the Agilent Lab Advisor help files.
This manual provides lists with the names of Error Messages, Not Ready
messages, and other common issues.
60
6
Test Functions
Thermostat Function Test
Pressure Test
62
65
68
Agilent Technologies
61
Test Functions
Thermostat Function Test
62
Test Functions
Thermostat Function Test
IZbeZgVijgZP8R
G^\]iEZai^ZgZaZbZci
AZ[iEZai^ZgZaZbZci
I^bZPb^cjiZhR
Figure 19
63
Test Functions
Thermostat Function Test
Suggested Actions
Ensure cover is installed correctly.
Ensure sufficient space is available for air circulation see Bench Space on
page 26.
Exchange the heater assembly.
64
Test Functions
Pressure Test
Pressure Test
For running a pressure test, please refer to the corresponding pump manual.
The pressure test may be used for testing the tightness of a valve installed in
the TCC.
CAUTION
as this will damage the valve. For example do not use 400 bar valve in a TCC in
combination with a 600 bar pump.
65
Test Functions
Column Thermostat Temperature Calibration
IZbeZgVijgZ
Y^[[ZgZcXZP8R
iZbeZgVijgZVi
bZVhjg^c\ed^ci
8dajbchZied^ciiZbeZgVijgZ
8Va^WgVi^dcViXgdhh"dkZged^ci(+8
IZbeZgVijgZP8R
Figure 20
66
Test Functions
Column Thermostat Temperature Calibration
67
Test Functions
Column Thermostat Temperature Calibration
Parts required
Description
Calibrated temperature measuring device
NOTE
NOTE
The figures in this procedure refer to a specific type of temperature sensor (Heraeus,
Quat340, quartz surface-temperature measurement sensor). Other sensors may require a
different fixing.
NOTE
Limits
After calibration, the measured temperature and the calibration temperature should be
within 0.5 C. The maximum deviation which can be adjusted is 1.6 C. If the measured
value and the calibration value differ by more than 1.6 C, this is an indication that a
problem exists, Column Thermostat Calibration Problems on page 69.
68
Test Functions
Column Thermostat Temperature Calibration
Hardware Failures
Probable hardware failures that can lead to a failed calibration procedure are:
Defective or wrongly calibrated measuring device.
Defective heater assembly.
Defective ambient-temperature sensor.
Defective CCM board.
NOTE
The figures below refer to a specific type of temperature sensor (Heraeus, Quat340, quartz
surface-temperature measurement sensor). Other sensors may require a different fixing.
69
Test Functions
Column Thermostat Temperature Calibration
3 Route the sensor wire through the slit in the leak tray.
70
7
Maintenance
Introduction to Maintenance and Repair
Warnings and Cautions
Overview of Maintenance
72
73
75
76
77
82
84
85
86
89
Agilent Technologies
71
Maintenance
Introduction to Maintenance and Repair
Simple Repairs
The column compartment is designed for easy repair. The most frequent
repairs such as change of column and column switching valve head parts can
be done from the front of the column compartment without removing the TCC
from the HPLC stack. These repairs are described in Maintenance on
page 71.
72
Maintenance
Warnings and Cautions
WA R N I N G
Module is partially energized when switched off, as long as the power cord is
plugged in.
Risk of stroke and other personal injury. Repair work at the module can lead to
personal injuries, e. g. shock hazard, when the module cover is opened and the
instrument is connected to power.
Never perform any adjustment, maintenance or repair of the module with the top
off when line power is still connected. Never plug the power line back in when cover
is removed.
WA R N I N G
areas.
WA R N I N G
goggles, safety gloves and protective clothing) as described in the material handling
and safety data sheet supplied by the solvent vendor, especially when toxic or
hazardous solvents are used.
73
Maintenance
Warnings and Cautions
CAUTION
CAUTION
74
Maintenance
Overview of Maintenance
Overview of Maintenance
Simple Repairs
Procedure
Typical Frequency
Changing Column
Identification Tags on page 77
Notes
75
Maintenance
Cleaning the Column Compartment
The column compartment case should be kept clean. Cleaning should be done
with a soft cloth slightly dampened with water or a solution of water and a
mild detergent. Make sure not to let liquid drip into your module.
WA R N I N G
76
Maintenance
Changing Column Identification Tags
6ciZccVh
8dajbc"^YZci^[^XVi^dciV\
8dajbcXa^e
Figure 21
Column-Identification System
When
If column is used on the opposite heat exchanger or a tag is added to a new column.
Parts required
Part number
Description
5062-8588
77
Maintenance
Changing Column Identification Tags
8dajbc"^YZci^[^XVi^dciV\
Figure 22
8dajbc"^YZci^[^XVi^dciV\
Figure 23
3 For columns with small diameter, a cable tie wrap should be used to fix the
column identification tag to the column. Assure that the tie wrap does not
block the front cover.
78
Maintenance
Replacing Head Parts of Column Switching Valve (G1316A/G1316B SL)
>hdaVi^dchZVa
GdidghZVa
HiVidgg^c\
HiVidg[VXZ
HiVidg]ZVY
Heg^c\h^YZ
h]dlhid
gZVg
HiVidghXgZlh
Figure 24
When
If valve leaks.
Tools required
Parts required
Description
For parts refer to Valve Options Overview on page 92.
79
Maintenance
Replacing Head Parts of Column Switching Valve (G1316A/G1316B SL)
2 Loosen each fixing stator screw two turns at a time. Remove bolts from
head.
NOTE
Valve Head, 8 Position/9 Port, High Pressure part number: 5067-4107 has no stator face.
80
Maintenance
Replacing Head Parts of Column Switching Valve (G1316A/G1316B SL)
NOTE
Valve Head, 8 Position/9 Port, High Pressure part number: 5067-4107 has no stator face.
10 Insert the stator screws in the stator head. Tighten the screws alternately
two turns at a time until the stator head is secure.
11 Reconnect the pump capillaries to the valve ports. Slide the waste tube into
the waste holder in the leak tray.
CAUTION
as this will damage the valve. For example do not use 400 bar valve in a TCC in
combination with a 600 bar pump.
81
Maintenance
Adding Heater and Cooling Devices (G1316B SL/G1316C SL Plus)
The addtional heater and cooling devices can be arranged in the G1316B SL in
various locations depending on the application needs . Some examples are
shown below.
Figure 25
NOTE
If the additional heater and cooling devices are used as shown above, the column
identification system cannot be used. If the column identification system is required, fix the
heater and cooling devices in the upper or lower locations or fix them right/left of the
current location.
The heater and cooling devices are mounted on a carrier that can be fitted to
the left and/or right heat exchangers.
82
Maintenance
Adding Heater and Cooling Devices (G1316B SL/G1316C SL Plus)
=ZViZmX]Vc\Zg
8Vgg^Zg[dg]ZViZgdgXddaZg
=ZViZgYZk^XZ
HXgZlYg^kZgIdgmdgEdh^Yg^k
Figure 26
For the heater device inlet capillary choose fittings which are compatible to
your column.
HlV\ZadX`XdbeVi^WaZXdajbch*%+*"))*)!e`d[&%!l^i][ZggjaZh
;^ii^c\hXgZladc\
HlV\ZadX`XdbeVi^WaZXdajbch!gZbdkVWaZ%&%%"'%-+
CjihZVai^\]i
Figure 27
83
Maintenance
Correcting Leaks
Correcting Leaks
When
If a leakage has occurred at the heat exchanger or at the capillary connections or at the column
switching valve.
Tools required
Tissue, pipette
Wrench 1/4 5/16 inch for capillary connections
NOTE
Depending on the column position or the use of additional heat-exchanger assemblies, the
view of Figure 28 on page 84 may vary.
AZV`hZchdgVhhZbWan
LVhiZ djiaZi
Figure 28
84
Maintenance
Replacing the Column Compartments Firmware
If new version solves problems of currently installed version or after exchange of the TCC main board
(CCM) the version on board is older than previous installed one.
Tools required
LAN/RS-232 Firmware Update Tool, or Instant Pilot G4208A or Control Module G1323B
Parts required
Description
Firmware, tools and documentation from Agilent web site
Preparations
NOTE
The G1316B SL requires firmware revision A.06.02 or higher (main and resident).
The G1316C SL Plus requires firmware revision A.06.10 or higher (main and resident).
85
Maintenance
Replacing Valve Heads (G1316C SL Plus)
Several optional valve heads are available for the G1316C, which can be
installed and exchanged easily.
Parts required
CAUTION
Part number
Description
5067-4107
5067-4108
Valve Damage
Using a low pressure valve on the high pressure side can damage the valve.
When using multiple column compartments as part of a method development
solution, make sure that the high pressure valve head is connected to the
autosampler and the low pressure valve head is connected to the detector.
For details, please refer to the Method Development Solution User and Installation
CAUTION
CAUTION
Valve properties are read from the valve head RFID tag during initialization of the
module. Valve properties will not be updated, if the valve head is replaced while the
module is on.
Selection of valve port positions can fail, if the instrument does not know the
properties of the installed valve.
Always switch off the instrument when replacing the valve head.
86
Maintenance
Replacing Valve Heads (G1316C SL Plus)
CAUTION
The valve actuator contains sensitive optical parts, which need to be protected from
dust and other pollutions. Pollution of these parts can impair the accurate selection of
valve ports and therefore bias measurement results.
Always install a valve head for operation and storage. For protecting the actuator, a
dummy valve head (part of transportation lock kit part number: G1316-67001) can be
used instead of a functional valve. Do not touch parts inside the actuator.
87
Maintenance
Replacing Valve Heads (G1316C SL Plus)
4 Put the new valve head onto the valve drive such that the 5 Screw the valve head onto the valve drive using the union
lobe fits to the groove.
nut.
6 Install all required capillary connections to the valve head. 7 Push the valve head until it snaps in and stays in the rear
position.
88
Maintenance
Preparing the G1316C SL Plus for Transportation
When
Tools required
Parts required
Part number
Description
G1316-67001
The module has been shipped with transportation locks, which must be used
for transportation protection. A transportation lock kit can be re-ordered
using
1 Remove the valve head as described in Replacing Valve
Heads (G1316C SL Plus) on page 86.
89
Maintenance
Preparing the G1316C SL Plus for Transportation
90
8
Parts and Materials for Maintenance
Valve Options Overview
92
93
95
97
109
110
Agilent Technologies
91
This overview gives a summary of the main parts and assemblies. More details
are available with each valve option in this chapter.
Table 7
Module
Valve Description
Valve
Rotor Seal
Stator
G1316A (#055)
G1316-68700
0101-0920 1
0100-1855 (Vespel)
0100-1854 (Tefzel)
0100-2233 (PEEK)
0100-1851 (Face)
0100-1850 (Head)
0100-1852 (Seal)
G1316A (#056)
0101-1051
0100-2087 (Vespel)
0101-2089
G1316A (#057)
G1316-68709
0101-1343 2
0101-1360
0101-1362
G1316B (#055)
0101-1420
0101-1409
0101-1417
G1316B (#057)
0101-1419
0101-1415
0101-1421
re-build kit 0101-1258 includes 3-groove rotor seal, stator face assy, isolation seal, instructions.
re-build kit 0101-1360 includes PEEK rotor seal, PEEK stator face, hex key.
Table 8
Kit
Kit Description
Valve Head
Rotor Seal
Stator
G4230A
5067-4108
5067-41131
5067-4112
G4230B
5067-4107
5067-4111
5067-4110
92
The use of these heater and cooling devices is described in Technical Notes or
in the Agilent 1200 Series Rapid Resolution LC System (1200 RRLC System)
manual.
Table 9
Item
Description
Part Number
G1316-80002
G1316-80003
G1316-80004
G1316-83200
0515-1052
93
Description
Part Number
5042-9918
G1316-89200
G1316-68706
A"h]VeZYegZ"Xdajbc]ZViZg
KdajbZ/&#+a
bdjciZYdcXVgg^Zg
ide[dgXdajbc&!Wdiidb[dg
Xdajbc'
J"h]VeZYEdhi"XdajbcXddaZg
KdajbZ/&#*a
bdjciZYdc8Vgg^Zg
]daZhidViiVX]XVgg^Zg
Figure 29
94
Table 11
Item
Description
Part Number
G1353-68700
0101-0920
G1316-44103
G1316-68708
G1313-87305
G1316-87300
0101-1258
Stator screws
1535-4857
Stator Head
0100-1850
Stator face
0100-1851
Stator ring
0100-1854
0100-1855
0100-2233
Isolation seal
0100-1852
95
+
*
)
(
'
&
Figure 30
96
Heg^c\h^YZ
h]dlhid
gZVg
Part Number
5067-4107
Stator Screws
1535-4857
Stator Head
5067-4110
5067-4111
Isolation Seal
0100-1852
)
(
'
&
Figure 31
97
Part Number
5067-4108
Stator Screws
1535-4857
Stator Head
5067-4112
3, 4
5067-4113
Isolation Seal
0100-1852
*
)
(
'
&
Figure 32
98
Table 14
Item
Description
Part Number
0101-1051
Stator face
0100-2089
0100-2087
Table 15
Item
Micro Column Switching Valve 2PS/6PT for G1316B SL, 600 bar
Description
Part Number
0101-1420
0101-1417
0101-1409
99
NOTE
Technical details can be found in the Technical Note that is supplied with the kit.
Table 16
Item
Description
Part Number
G1316-68709
Rebuild kit, includes PEEK rotor seal, PEEK stator face, hex key
0101-1360
2PS/10 PT valve
0101-1343
0101-1361
Stator face
0101-1362
G1316-68711
Table 17
Item
Table 18
From
Micro Column Switching Valve 2PS/10PT 600 bar for G1316B SL, 600 bar
Description
Part Number
0101-1419
0101-1421
0101-1415
ID [mm]
Length [mm]
Qty
Part number
Valve (port 2)
0.17
700
5065-9932
Remark
Capillaries
ALS 1
100
From
To
ID [mm]
Length [mm]
Qty
Part number
Remark
Valve (port 3)
TCC 3 l (In)
0.17
105
5021-1816
TCC 2 3 l (Out)
Column 1
0.17
105
5021-1816
Column 1
Valve (port 6)
0.17
105
5021-1816
Column 1
Valve (port 6)
0.17
200
5065-9931
Valve (port 7)
Detector (In)
0.17
280
5021-1818
Valve (port 1)
TCC 6 l (In)
0.17
105
5021-1816
TCC ** 6 l (Out)
Column 2
0.17
105
5021-1816
Column 2
Valve (port 8)
0.17
105
5021-1816
Column 2
Valve (port 8)
0.17
200
5065-9931
Valve (port 5)
0.17
105
5021-1816
Regeneration pump
Valve (port 4)
0.25
800
5065-9930
Valve (port 9)
Waste
0.6
2000
5062-2463
PTFE
5062-2418
10/pk
5062-8541
10/pk
10
5065-4454
10/pk
10
5065-9967
10/pk
front ferrule
5180-4108
10/pk
back ferrule
5180-4114
10/pk
0890-1763
8710-1930
8710-2462
8710-2391
0.18
1500
ALS - Autosampler
101
Accessory Kits
102
Description
Part Number
G1316-68705
5062-8588
5063-6526
5062-2463
5181-1516
8710-0510
8710-2409
G1316-87300
Description
Part Number
G1316-68725
5062-8588
5063-6526
5062-2463
5181-1516
8710-0510
8710-2409
G1316-87309
5022-2186
5001-3702
Description
Part Number
G1316-68735
5062-8588
5063-6526
5062-2463
5181-1516
8710-0510
8710-2409
103
Description
Part Number
8710-2509
5965-0028
G1316-87300
G1316-87321
G1316-87323
Item
Description
Part Number
G1316-83200
G1316-80002
G1316-80003
G1316-80004
G1316-68716
For items * see also Heater and Cooling Devices for G1316B SL/G1316C SL
Plus on page 93.
104
Description
Part Number
G1367-87303
G1315-87339
G1316-87319
G1316-87318
G1316-87317
G1316-87316
G1316-87315
G1316-87314
G1316-87313
G1316-87312
G1316-87327
G1316-87309
G1315-87307
Description1
Part Number
5067-4601
5067-1595
5067-1596
5067-1597
5190-1431
5190-1432
5190-1433
5190-1434
105
Description1
Part Number
5190-1435
5190-1436
for capillary kit contents, please refer to the Method Development Solution User and
Installation Guide (G4230-90000)
106
Description
Part Number
G1316-68745
5062-8588
5063-6526
5062-2463
8710-0510
8710-2409
8710-2509
5965-0028
5001-3702
G1316-68721
G1316-87300
G1316-87321
G1316-87323
Description
where used
Part Number
G1316-68721
column to cell
G1312-87304
switching capillary
G1316-27301
adapter capillary
G1316-87304
WPS to valve
G1316-87305
valve to heatexchanger
G1316-87306
column to cell
G1316-87312
column to cell
G1316-87313
valve to detector
G1316-87326
G1367-87303
G4240-43200
valve to waste
5022-6503
107
Figure 33
108
Description
Part Number
G1316-67001
Plastic Parts
Table 28
Plastic Parts
Item
Description
Part Number
G1316-68714
G1316-68724
G1316-68754
5042-8901
'
Figure 34
Plastic Parts
109
Leak Parts
Table 29
Item
Description
Part Number
Leak funnel
5041-8388
G1316-42300
G1316-60002
5062-2463
&
'
(
Figure 35
110
Leak Parts
Item
Desription
Part Number
G1316-68722
G1316-67000
Capillary Guide
G1316-42303
&
Figure 36
Capillary Guide
111
112
9
Appendix
Safety Symbols
114
117
118
119
120
Solvent Information
121
123
Agilent Technologies
113
Appendix
Safety Symbols
Safety Symbols
Safety Symbols
Table 31
Safety Symbols
Symbol
Description
The apparatus is marked with this symbol when the user should refer to the instruction manual
in order to protect risk of harm to the operator and to protect the apparatus against damage.
Indicates dangerous voltages.
Indicates a protected ground terminal.
Indicates eye damage may result from directly viewing the light produced by the deuterium lamp
used in this product.
The apparatus is marked with this symbol when hot surfaces are available and the user should
not touch it when heated up.
WA R N I N G
A WARNING
alerts you to situations that could cause physical injury or death.
Do not proceed beyond a warning until you have fully understood and met the
indicated conditions.
CAUTION
A CAUTION
alerts you to situations that could cause loss of data, or damage of equipment.
Do not proceed beyond a caution until you have fully understood and met the
indicated conditions.
114
Appendix
Safety Symbols
WA R N I N G
General
This is a Safety Class I instrument (provided with terminal for protective
earthing) and has been manufactured and tested according to international
safety standards.
Operation
Before applying power, comply with the installation section. Additionally the
following must be observed.
Do not remove instrument covers when operating. Before the instrument is
switched on, all protective earth terminals, extension cords,
auto-transformers, and devices connected to it must be connected to a
protective earth via a ground socket. Any interruption of the protective earth
grounding will cause a potential shock hazard that could result in serious
personal injury. Whenever it is likely that the protection has been impaired,
the instrument must be made inoperative and be secured against any intended
operation.
Make sure that only fuses with the required rated current and of the specified
type (normal blow, time delay, and so on) are used for replacement. The use of
repaired fuses and the short-circuiting of fuse holders must be avoided.
115
Appendix
Safety Symbols
Some adjustments described in the manual, are made with power supplied to
the instrument, and protective covers removed. Energy available at many
points may, if contacted, result in personal injury.
Any adjustment, maintenance, and repair of the opened instrument under
voltage should be avoided whenever possible. When inevitable, this has to be
carried out by a skilled person who is aware of the hazard involved. Do not
attempt internal service or adjustment unless another person, capable of
rendering first aid and resuscitation, is present. Do not replace components
with power cable connected.
Do not operate the instrument in the presence of flammable gases or fumes.
Operation of any electrical instrument in such an environment constitutes a
definite safety hazard.
Do not install substitute parts or make any unauthorized modification to the
instrument.
Capacitors inside the instrument may still be charged, even though the
instrument has been disconnected from its source of supply. Dangerous
voltages, capable of causing serious personal injury, are present in this
instrument. Use extreme caution when handling, testing and adjusting.
When working with solvents please observe appropriate safety procedures
(e.g. goggles, safety gloves and protective clothing) as described in the material
handling and safety data sheet by the solvent vendor, especially when toxic or
hazardous solvents are used.
116
Appendix
The Waste Electrical and Electronic Equipment Directive
Abstract
The Waste Electrical and Electronic Equipment (WEEE) Directive
(2002/96/EC), adopted by EU Commission on 13 February 2003, is
introducing producer responsibility on all electric and electronic appliances
starting with 13 August 2005.
NOTE
This product complies with the WEEE Directive (2002/96/EC) marking requirements. The
affixed label indicates that you must not discard this electrical/electronic product in
domestic household waste.
Product Category:
With reference to the equipment types in the WEEE Directive Annex I, this product is
classed as a Monitoring and Control Instrumentation product.
NOTE
117
Appendix
Lithium Batteries Information
WA R N I N G
Lithium batteries may not be disposed-off into the domestic waste. Transportation of
discharged Lithium batteries through carriers regulated by IATA/ICAO, ADR, RID,
IMDG is not allowed.
Danger of explosion if battery is incorrectly replaced.
Discharged Lithium batteries shall be disposed off locally according to national
manufacturer.
WA R N I N G
WA R N I N G
Lithiumbatteri - Eksplosionsfare.
Ved udskiftning benyttes kun batteri som anbefalt av apparatfabrikanten.
Brukt batteri returneres appararleverandoren.
NOTE
118
Bij dit apparaat zijn batterijen geleverd. Wanneer deze leeg zijn, moet u ze niet weggooien
maar inleveren als KCA.
Appendix
Radio Interference
Radio Interference
119
Appendix
Sound Emission
Sound Emission
Manufacturers Declaration
This statement is provided to comply with the requirements of the German
Sound Emission Directive of 18 January 1991.
This product has a sound pressure emission (at the operator position) < 70 dB.
Sound Pressure Lp < 70 dB (A)
At Operator Position
Normal Operation
According to ISO 7779:1988/EN 27779/1991 (Type Test)
120
Appendix
Solvent Information
Solvent Information
Flow Cell
To protect optimal functionality of your flow-cell:
Avoid the use of alkaline solutions (pH > 9.5) which can attack quartz and
thus impair the optical properties of the flow cell.
If the flow cell is transported while temperatures are below 5 degree C, it
must be assured that the cell is filled with alcohol.
Aqueous solvents in the flow cell can built up algae. Therefore do not leave
aqueous solvents sitting in the flow cell. Add a small % of organic solvents
(e.g. acetonitrile or methanol ~5%).
Use of Solvents
Observe the following recommendations on the use of solvents.
Brown glass ware can avoid growth of algae.
Small particles can permanently block capillaries and valves. Therefore
always filter solvents through 0.4 m filters.
Avoid the use of the following steel-corrosive solvents:
Solutions of alkali halides and their respective acids (for example,
lithium iodide, potassium chloride, and so on),
High concentrations of inorganic acids like sulfuric acid and nitric acid,
especially at higher temperatures (if your chromatography method
allows, replace by phosphoric acid or phosphate buffer which are less
corrosive against stainless steel),
Halogenated solvents or mixtures which form radicals and/or acids, for
example:
2CHCl3 + O2 > 2COCl2 + 2HCl
This reaction, in which stainless steel probably acts as a catalyst, occurs
quickly with dried chloroform if the drying process removes the
stabilizing alcohol,
121
Appendix
Solvent Information
Chromatographic grade ethers, which can contain peroxides (for
example, THF, dioxane, di-isopropylether) such ethers should be filtered
through dry aluminium oxide which adsorbs the peroxides,
Solvents containing strong complexing agents (e.g. EDTA),
Mixtures of carbon tetrachloride with 2-propanol or THF.
122
Appendix
Agilent Technologies on Internet
For the latest information on products and services visit our worldwide web
site on the Internet at:
http://www.agilent.com
Select Products/Chemical Analysis
It will provide also the latest firmware of the Agilent 1200 Series modules for
download.
123
Index
Index
Agilent
Diagnostic Software 22, 60
Lab Advisor Software 22, 60
on internet 123
algae 121, 121
ambient operating temperature 27
ambient non-operating temperature 27
dead volume 28
delivery checklist 34
Diagnostic
software 22, 60
dimensions 27
heaterexchanger
pre-column 30
heating concept 11
humidity 27
B
battery
safety information
bench space 26
118
C
calibration
temperature 56, 66
column switching valve (optional)
description 15
precolumn backflushing 17
column switching valve (optional)
two column selection 16
column clip 50
column identification 13
tag 49
column
changing column and tags 77
cool-down time 28
cooling concept 11
cooling device
post column 30
I
E
electrical connections
descriptions of 18
electronic waste 117
electrostatic discharge (ESD)
environment 26
error messages 56
external measuring device
information 68
74
F
features
GLP 28, 29
instrument layout 21
safety and maintenace 28
flow cell 121
solvent information 121
frequency range 27
function test
failed 64
result 62
G
GLP features
28
installation
bench space 26
capillaries and waste tubings 46
column 45, 45
flow connections 44
module 38
power cords 25
temperature sensor 69
unpacking 34
instrument layout 21
internet 123
introduction
concept of heating and cooling 11
system overview 11
L
Lab Advisor
software 22, 60
leaks, correcting 84
line frequency 27
line voltage 27
lithium batteries 118
N
non-operating altitude 27
non-operating temperature 27
124
Index
operating Altitude 27
operating temperature 27
optimization 51
optimizing the performance 52
P
parts identification
leak panels 110
parts identification
plastics 102
performance
Optimization 51
optimizing 52
specifications and features
physical specifications 27
physical specifications 27
post-column
cooling device 30
power consideration 24
power consumption 27
power cords 25
pre-column
heatexchanger 30
28
R
radio interference 119
repairs
changing the column 77
cleaning the instrument 76
column switching valve parts 79
correcting leaks 84
introduction 72
overview 75
types simple/internal 72
warnings and cautions 72
T
tag
U
unpacking 34
User Interfaces
diagnosic tests
59
V
verification
temperature 56
voltage range 27
W
warm-up time 28
waste
electrical and electronic
equipment 117
WEEE directive 117
weight 27
installation 49
temperature verification
principle 69
temperature calibration
description 66
problems 69
procedure 68
temperature verification
external measuring device 68
temperature
range 28
tests
available tests vs interface 59
test
thermostat function test failed 64
125
www.agilent.com
In This Book
Printed in Germany
09/2008
*G1316-90011*
*G1316-90011*
G1316-90011
Agilent Technologies