World J Gastrointest Oncol 2016 December 15; 8(12): 819-825

ISSN 1948-5204 (online)

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DOI: 10.4251/wjgo.v8.i12.819

2016 Baishideng Publishing Group Inc. All rights reserved.

ORIGINAL ARTICLE
Retrospective Study

Signet ring colorectal carcinoma: Do we need to improve

the treatment algorithm?
Anup Sunil Tamhankar, Parag Ingle, Reena Engineer, Munita Bal, Vikas Ostwal, Avanish Saklani
Anup Sunil Tamhankar, Parag Ingle, Department of Surgical
Oncology, Tata Memorial Centre, Mumbai, Maharashtra 400012,
India

Associate Professor, Department of Gastro-Intestinal Surgery,

Tata Memorial Centre, Mumbai, Maharashtra 400012,
India. [email protected]
Telephone: +91-996-9506719
Fax: +91-222-4177000

Reena Engineer, Department of Radiotherapy, Tata Memorial

Centre, Mumbai, Maharashtra 400012, India

Received: April 2, 2016

Peer-review started: April 7, 2016
First decision: June 6, 2016
Revised: July 11, 2016
Accepted: September 13, 2016
Article in press: September 18, 2016
Published online: December 15, 2016

Munita Bal, Department of Surgical Pathology, Tata Memorial

Centre, Mumbai, Maharashtra 400012, India
Vikas Ostwal, Department of Medical Oncology, Tata Memorial
Centre, Mumbai, Maharashtra 400012, India
Avanish Saklani, Department of Gastro-Intestinal Surgery, Tata
Memorial Centre, Mumbai, Maharashtra 400012, India
Author contributions: All authors contributed to the manuscript.

Abstract

Institutional review board statement: We hereby declare that

the institutional review board has inspected and approved this
retrospective study.

AIM
To elaborate about this peculiar variant from a tertiary
cancer center from India.

Informed consent statement: We hereby declare that waiver

of consent has been obtained for this retrospective study from
Institutional Review Board due to lack of direct patient contact.
Also this study doesnt disclose the identity or private information
of any of the study patients.

METHODS
Its a retrospective study (2011-2014) of all patients
diagnosed with signet ring colo-rectal cancer (SRCC).
Various clinico-pathological variables were studied.

Conflict-of-interest statement: No potential conflicts of interest

relevant to this article were reported.

RESULTS
One hundred and seventy consecutive patients with
SRCC were diagnosed (11.4% of all colorectal cancers).
Median Age of the cohort was 41 years. Most common
location was recto-sigmoid area (54.7%). Majority
patients presented in stage III and IV (91.2%). Most of
the stage IV patients had isolated peritoneal metastases
(86.5%). Colonic tumors had higher incidence of peri
toneal metastases (91.8% vs 83.3%) as well as isolated
peritoneal recurrences (37.5% vs 16.7%) than rectal
primaries. Thirty-seven point five percent of patients
recurred after curative surgery. Amongst them 63.63%
patients had isolated peritoneal recurrences. Circum
ferential resection margin (CRM) was involved in 17.9%
patients. Median relapse free survival (RFS) and overall

Open-Access: This article is an open-access article which was

selected by an in-house editor and fully peer-reviewed by external
reviewers. It is distributed in accordance with the Creative
Commons Attribution Non Commercial (CC BY-NC 4.0) license,
which permits others to distribute, remix, adapt, build upon this
work non-commercially, and license their derivative works on
different terms, provided the original work is properly cited and
the use is non-commercial. See: http://creativecommons.org/
licenses/by-nc/4.0/
Manuscript source: Invited manuscript
Correspondence to: Dr. Avanish Saklani, MS, FRCS,

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December 15, 2016|Volume 8|Issue 12|

Tamhankar AS et al . Signet ring cell colorectal carcinoma

survival (OS) of the cohort were 14.9 and 18.13 mo

respectively. CRM involvement, colonic primary were
associated with poorer RFS and OS.

MATERIALS AND METHODS

st

All patients diagnosed with colorectal carcinoma from 1

st
January 2011 to 31 December 2013, registered under
the Department of Gastro-intestinal Oncology services,
Tata Memorial Centre, were included. The data was
collected retrospectively from Electronic database as
well as case files from Department of Surgical Oncology.
The histopathology specimens of all these patients were
reviewed at Department of Surgical pathology, Tata
Memorial Centre. Signet ring cell colorectal cancers were
defined as per WHO criteria (AC with more than 50%
of signet-ring cells). Patients were staged as per AJCC
th
classification (7 edition). Response to neoadjuvant
chemoradioatherapy (NACTRT) was assessed as per
RECIST criteria. The decision about the same was taken
in the multidisciplinary meeting held for every patient.
Pathological complete response was defined as absence
of viable tumor cells in the primary, the lymph nodes
and peri-rectal soft tissue. Circumferential resection
margin (CRM) positivity was defined as presence of
viable tumor cells at or within 1 mm of it. Follow up
data was obtained from electronic medical records
and/or telephonic questionnaire. Recurrences were
based on biopsy or strong clinico-radiological evidence.
Peritoneal metastases or recurrences constituted
peritoneal deposits, malignant ascites, omental deposits
and ovarian deposits. Relapse free survival (RFS) was
assessed from the date of cancer directed surgery to
date of recurrence. Overall survival (OS) was measured
from the date of diagnosis of malignancy to date of
death. SPSS-21 (IBM corporation) was used for the
statistical analysis. Categorical variables were compared
2
with test. Survival functions were analyzed with
Kaplan Meir curves and compared with log rank test.

CONCLUSION
SRCC has predilection for peritoneal dissemination. More
aggressive and/or extended chemotherapy schedules
as well as prophylactic hyperthermic intra-peritoneal
chemotherapy at the time of primary surgery may be
attempted in these patients.
Key words: Colorectal cancer; Signet ring cell car
cinoma; Peritoneal metastases; Hyperthermic intraperitoneal chemotherapy
The Author(s) 2016. Published by Baishideng Publishing
Group Inc. All rights reserved.

Core tip: The incidence of Signet Ring Colo-Rectal

Cancer appears to be higher in Indian subcontinent
than the world literature. It has predilection for
peritoneal lining. It affects younger age group. Majority
cases present in stage III and IV. Recto-sigmoid region
is affected commonly. The most common metastatic
site and site of recurrence is peritoneal cavity. Probably
it should be treated with a different protocol than the
conventional adenocarcinoma with focus on aggressive
peritoneal cytoreductions and hyperthermic intraoperative intraperitoneal chemotherapy (HIPEC). Further
research is needed to evaluate molecular biology of this
variant and utility of prophylactic HIPEC during curative
surgery.
Tamhankar AS, Ingle P, Engineer R, Bal M, Ostwal V, Saklani
A. Signet ring colorectal carcinoma: Do we need to improve
the treatment algorithm? World J Gastrointest Oncol 2016;
8(12): 819-825 Available from: URL: http://www.wjgnet.
com/1948-5204/full/v8/i12/819.htm DOI: http://dx.doi.
org/10.4251/wjgo.v8.i12.819

RESULTS
st

INTRODUCTION
Colorectal cancer (CRC) is one of the most common
[1]
cancers worldwide . There are three subtypes
described in the literature based on the amount and
location of mucin in the tumor. These are conventional
adenocarcinoma (AC), mucinous carcinoma (MC) and
[2,3]
signet ring cell carcinoma (SRCC) . SRCC constitutes
[4-9]
1% of all colorectal carcinomas . It is an aggressive
variant which affects younger population and has poorer
[5]
prognosis . The literature explaining the biology as well
as the optimum treatment algorithm of this particular
variant is scarce due to its low incidence. So we look
into the incidence, demographics, clinico-radiological
presentation and outcome of treatment of this peculiar
variant from a tertiary cancer centre from India (Tata
Memorial Centre, Mumbai).

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st

From 1 January 2011-31 December 2013, 1487

patients with colorectal cancer got registered under the
department of Gastrointestinal Services Tata Memorial
Centre. Amongst them, signet ring cell carcinoma was
diagnosed in 170 consecutive patients (11.4%). Follow
up of 18 of 170 patients (10.58%) was inadequate (< 1
mo) (Table 1). Median Age of the cohort was 41 years.
Males were affected nearly twice more than females (M:
F = 1.8:1). Most tumors were located in the rectum and
sigmoid colon (Rectum: 41.2% and Sigmoid: 13.5%).
Majority patients presented in stage III (51.8%) and
stage IV (39.4%). Most of the stage IV patients had
isolated peritoneal metastases (58/67, 86.5%) (Table 2).
Curative surgery was feasible only in 51.76% (88/170)
patients. Thirty-seven point five percent (33/88)
patients recurred after curative surgery. Twenty-one
thirty-thirds (63.63%) patients had isolated peritoneal
recurrences (Table 3). Most patients had high nodal
burden, pN1 being 23.2% (22/95), pN2 being 57.9%
(55/95). Amongst node positive patients, 66.3% (53/77)

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Tamhankar AS et al . Signet ring cell colorectal carcinoma

Table 1 Demographic parameters
Parameter

Table 3 Pattern of recurrence after curative surgery

Statistics

Total No.
Sex ratio
Male
Female
Age (median), yr
Stage, n (%)
II
III
IV
Not available
Location, n (%)
Right colon
Transverse colon
Descending colon
Sigmoid colon
Rectum
Appendix
Not available

Locoregional
Distant
Isolated peritoneal
Peritoneal + second primary
Local + peritoneal

110
60
41
6 (3.5)
88 (51.8)
67 (39.4)
9 (5.3)

Site of metastases

Table 4 Factors affecting overall survival

49 (28.8)
13 (7.6)
11 (6.5)
23 (13.5)
70 (41.2)
1 (0.6)
3 (1.8)

Parameter
Location (After curative surgery)
Colon
Rectum
CRM
Positive
Negative
Metastases
Peritoneal
Non-peritoneal

n (%)

OS (mo)

Significance

32.3
40.1

0.058

19.9
41.5

0.018

14.85
11.14

0.729

CRM: Circumferential resection margin; OS: Overall survival.

1 (1.5)
1 (1.5)
58 (86.5)
2 (3.1)
5 (7.4)

males and females respectively. It is a cause of 8%

and 9% of cancer related deaths in males and females
[1]
respectively . Several histological subtypes have
[2,3]
been reported . It has two different subgroups apart
from classical AC. They are classified based on varying
amounts of signet-ring cell and/or mucinous component.
Signet-ring cell carcinoma (SRCC) is characterized by
intra-cytoplasmic mucin which displaces the nucleus
aside. MC is characterized by extracellular mucin pools.
SRCC or MC (defined as carcinoma with more than 50%
of signet-ring cells or mucinous component, respectively
as per WHO classification) constitutes approximately
1% or 5%-15% of CRC cases, respectively in the world
[4-9]
literature . As compared to the world literature, the
incidence of SRCC is much higher in our study (11.4%
vs 1%). The median age of the cohort in our study
was also lower than world literature (41 years vs 50-55
[5,6,10,11]
years)
. This could represent either a referral bias
being a tertiary cancer centre in India or definite distinct
disease biology in the Indian population. Further studies
regarding the demographic profile of this particular
variant in Indian population are under consideration
currently.
The literature is divided about the most common
site of colorectal cancer in young population with some
[12]
indicating proximal colon
and others suggesting it to
[13,14]
be recto-sigmoid region
. In our study, rectum and
sigmoid colon region was most commonly affected.
This may be related to preferential referral of locally
advanced rectal cases to our institute. One of the studies
has shown that colorectal cancers affecting younger age
group (< 40 years) have significantly higher incidence of
signet ring cell cancer. Such tumors also affect rectosig
moid area more commonly than rest of the colon in

had perinodal extension. The rate of lymph node

metastases and lympho-vascular invasion increased
progressively with increasing pathological T stage.
Median RFS and OS of the cohort were 14.9 mo
and 18.13 mo respectively. OS of peritoneal and nonperitoneal metastases were equivalent (16 mo vs 13
mo, P = 0.729) (Table 4).
Forty-eight rectal cancers were operated. Data
for patients undergoing NACTRT was available for 37
cases only. Pathological complete response was seen
in 21.6% (8/37) patients. CRM was involved in 17.9%
(7/39) patients (data on CRM was not available for 9
cases). CRM involvement was associated with poorer
RFS (15 mo vs 37.2 mo, P = 0.060) and OS (19.9 mo
vs 41.5 mo, P = 0.018) as compared to patients with
uninvolved CRM (Tables 4 and 5).
The location of primary had a significant impact
on the clinico-pathological outcome of the patient. As
compared to rectal primaries, colonic tumors had higher
incidence of peritoneal metastases (83.3% vs 91.8%,
P = 0.074) as well as isolated peritoneal recurrences
(16.7% vs 37.5%, P = 0.062). Colonic primaries were
associated with poorer OS than rectal tumors after
curative resection (32.298 mo vs 40.089 mo, P = 0.058)
and RFS (24.74 mo vs 34.02 mo, P = 0.048) (Table 6).

DISCUSSION
CRC is one of the most common cancers worldwide.
Worldwide, it leads to 10% and 9.2% of cancers in

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4 (12.12)
4 (12.12)
21 (63.63)
2 (6.06)
2 (3.4)

Regional recurrences: Regional lymph node recurrences; Distant

recurrences: Non-regional lymph nodal and visceral recurrences.

Table 2 Pattern of metastases in stage IV patients

Liver
Lung
Isolated peritoneal
Retroperitoneal lymphnodes
Others

n (%)

Pattern of recurrence

170

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Tamhankar AS et al . Signet ring cell colorectal carcinoma

Table 5 Factors affecting relapse free survival in operated
patients

Table 6 Impact of location on outcome

Parameter

Recurrence after curative resection

Peritoneal, n (%)
15/40 (37.5)
Non-peritoneal, n (%)
3/40 (7.5)
Pattern of Metastases at presentation
Peritoneal, n (%)
45/49 (91.8)
Non-peritoneal, n (%)
4/49 (9.2)
RFS (mo)
24.74
Overall Survival (mo)
26.011
OS after curative surgery (mo)
32.298

Parameter
CRM
Positive
Negative
Location
Colon
Rectum

RFS (mo)

Significance

15.003
37.202

0.060

24.74
34.02

0.048

RFS: Relapse free survival; CRM: Circumferential resection margin.

Rectum

Significance

8/48 (16.7)
7/48 (14.6)

0.062

15/18 (83.3)
3/18 (16.7)
34.02
30.32
40.089

0.074
0.048
0.062
0.058

RFS: Relapse free survival; OS: Overall survival.

[15]

young patients .
SRCC has been associated with peculiar genomic
changes such as high-degree microsatellite instability
(MSI-high) (up to 40%), high-frequency of CpG island
methylator phenotype, higher methylation level of long
interspersed nucleotide element-1 and frequent BRAF
[8,16-20]
mutation and low COX-2 expression
. Due to high
[21]
frequency of MSI-H mutations
and associated poor
prognosis, tumors with signet ring histo-morphology
are recommended to be screened for MSI-H mutations
[22]
as per revised Bethesda guidelines . The serrated
adenoma-carcinoma pathway has been proposed for
[23]
development of these tumors. Terada et al
found
that epithelial membrane antigen was downregulated
[24]
in colorectal SRCC. Kim et al
showed that focal loss
of epithelial cell adhesion molecule was associated with
development of SRCC in colonocytes. These molecular
changes may be related to preferential peritoneal
spread of this subtype. Currently the studies are under
consideration at our institute to assess genomic changes
related to this specific phenotype which may be the
cause of higher incidence of signet ring colorectal cancer
in Indian population than the world literature.
Our study revealed that, though SRCC has an
aggressive biology in general, it seems to respond well
to NACTRT with pathological complete response rate of
21%. Literature assessing response of SRCC to NACTRT
is scarce due to low incidence worldwide. Jayanand et
[25]
al showed that these tumors respond well to RT with
high pathological complete response rates. It may be
related to their aggressive nature and higher mitotic
index. So potentially NACTRT should be included in
the treatment protocol of rectal SRCC for improved
outcomes.
Patients with SRCC are more likely to present in
advanced stages (Stage III/IV) than AC. SRCC patients
more often present with metastatic disease and are
more likely to develop peritoneal metastases. This may
be related to their peculiar molecular origin which is yet
to be proven. It is also shown that SRCC metastasizes
to the lymph nodes, whereas AC metastasizes primarily
[6,9,11]
to the liver
. Our study also showed similar findings.
SRCC has been associated with a poor prognosis
[5,6,10,11]
compared with AC
. Studies have shown that
peritoneal metastases of SRCC are associated with a
poorer prognosis, and survival is even worse if other

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Colon

[26]

organs are also affected . But in our study, patients

with peritoneal metastases had similar OS as compared
to those with non-peritoneal metastases. This may
be due to small sample size of the study. Often, these
metastases cannot be treated with curative intent. As
of now, curative surgery is an option mainly limited to
liver and lung metastases, which are the most common
metastatic sites in AC patients. Systemic chemotherapy
for peritoneal metastases may not yield the same
results compared with hematogenous metastases due
to blood-peritoneal barrier. As a result, outcome is poor
[27]
in advanced SRCC cases .
The incidence of synchronous and metachronous
peritoneal metastases in colorectal carcinoma (AC)
seems to be in the range of 4%-5% (much lower than
[26,28]
with SRCC)
. Studies have revealed that peritoneal
carcinomatosis among patients with metastatic colore
ctal cancer is associated with a 30% reduction in overall
[29]
survival (10.7 mo vs 17.6 mo) . The overall survival of
these patients is found to be less than 6 mo despite the
[30,31]
use of 5FU and leucovorin based chemotherapy
.
But palliative surgery and systemic chemotherapy,
together have been shown to improve survival upto 12
[29,32]
mo in patients with isolated peritoneal metastases
.
Hyperthermic intra-operative intra-peritoneal chemo
therapy (HIPEC) has shown promising results for peri
[29]
toneal metastases of colorectal origin . Verwaal et
[29]
al reported outcome of 1427 patients with peritoneal
metastases of colorectal origin treated with cytoreductive
surgery (CRS) and HIPEC. Peri-operative morbidity
and mortality were 34% and 3% respectively. Median
hospital stay was 16 d. Median PFS was 15 mo and OS
was 33 mo. Three- and five-year survival rates were
46% and 31% respectively. So authors concluded
that CRS and HIPEC seems to be safe and beneficial
[33]
in peritoneal metastases of colorectal origin . But
literature assessing benefit of HIPEC for SRCC is
[34,35]
scarce and controversial with studies denying
and
[36]
implying benefit of HIPEC in this subgroup. But these
reports are retrospective and are fraught with small
sample sizes.
[37]
Recently, Hao et al have proposed a study assess
ing the benefit of monoclonal antibody blocking EpCAM
in CRC. This may be relevant in the further management

822

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Tamhankar AS et al . Signet ring cell colorectal carcinoma

of SRCC as EpCAM also has altered expression in this
subtype.
[38]
Klaver et al
have proposed a randomized con
trolled trial (COLOPEC) for assessing benefit of prophy
lactic HIPEC in patients at high risk of peritoneal carcino
matosis. They have included patients (non-metastatic)
with T4 disease or on table tumor site perforation
for prophylactic HIPEC followed by routine adjuvant
chemotherapy. It has been postulated in assumption
that very few patients with peritoneal carcinomatosis
become eligible for CRS and HIPEC; as a result they
have poor prognosis. So if a prophylactic HIPEC reduces
the occurrence of peritoneal metastases in future, it
may result in benefit in OS. The investigators have
not considered signet ring cell pathology as inclusion
criteria for the study; probably because of low incidence
(1%-2%) of it in the western literature. A similar study
may be considered in Indian patients with signet ring
cell carcinoma to assess benefit of prophylactic HIPEC
at the time of primary surgery as it has a peculiar
tendency for isolated peritoneal recurrences and the
incidence of this particular histopathological subtype
seems to be higher in them (11.4%) as suggested by
present study.
It is unclear whether different histological subtypes
should influence treatment decisions, since it is often
not addressed in clinical trials. In the literature, studies
concerning outcome after adjuvant or palliative chemo
therapy for SRCC are rare. However, due to the aggres
sive behavior and high incidence of SRCC in young
patients, it is imperative to develop understanding of
potential adjuvant treatment options as it is likely to
alter quality of life and have significant socio-economic
impact. Colonic SRCC are more likely to have peritoneal
dissemination and poorer survival than rectal SRCC.
So more aggressive treatment options, like HIPEC
may be useful in these patients at the time of primary
surgery or after peritoneal limited recurrence in order
to improve survival and quality of life. This can only be
addressed in a randomized control trial setting. Due to
high nodal disease burden and high incidence of failure
after curative surgery (up to 40%), more extended
and/or aggressive adjuvant chemotherapy options
should also be explored in this subset of population
which is younger and is likely to tolerate the aggressive
treatment better.
Signet ring colorectal cancer has poor prognosis. It
has a higher incidence in Indian subcontinent. It affects
young patients and has predilection for peritoneal
dissemination.
Isolated peritoneal metastases as well as isolated
peritoneal recurrences are very frequent in these
patients. SRCC responds well to radiation. So whenever
indicated, neoadjuvant radiation should be included in
the treatment protocol for rectal SRCC.
More aggressive and/or extended chemotherapy
schedules as well as prophylactic HIPEC at the time of
primary surgery, especially for colonic tumors, should
be explored in a trial setting in order to improve dismal

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survival in these patients.

COMMENTS
COMMENTS
Background

Signet ring colorectal cancer (SRCC) is a subtype of colorectal adenocarcinoma.

It tends to affect younger age group. Most of the patients present in stage III
or IV. The most common site affected is rectosigmoid region. It has a peculiar
affection for peritoneal lining. Most of the metastases and recurrences happen
exclusively in the peritoneal cavity. Visceral metastases are rare. Average
prognosis of these patients is poor. There is no effective adjuvant or palliative
treatment for this entity. Early studies in the field of cytoreductionandhyperthermic
intra-operative intraperitoneal chemotherapy (HIPEC) have shown promising
results and prolongation of survival in peritoneal carcinomatosis of colorectal
cancer. The trials are underway to test the impact of prophylactic HIPEC during
primary surgery for cT4N1/2 diseases. Since SRCC has a different natural course
than the conventional adenocarcinoma of colon, it may be worthwhile to evaluate
the possible role of extended chemotherapy or prophylactic HIPEC at the time of
curative surgery for SRCC.

Research frontiers

Currently trials are underway (COLOPEC and Prophylochip) to assess efficacy

of prophylactic HIPEC in high risk colorectal cancers to prevent occurrence of
peritoneal metastases and prolongation of survival. Though aggressive, SRCC
has shown its peculiar nature to remain confined to peritoneal cavity in majority
patients. This makes it a potential target for peritoneum directed therapies
(Cytoreduction and HIPEC). Also monoclonal antibodies blocking EpCAM are
being evaluated in CRC. This may be relevant in the further management of
SRCC as EpCAM also has altered expression in this subtype.

Innovations and breakthroughs

Cytoreduction and HIPEC has shown survival benefit in peritoneal carcinomatosis

of colorectal origin in a large randomized trial by Verwaal et al SRCC has not been
evaluated widely in the western literature, probably due to lower incidence. But in
Indian subcontinent, the incidence of this disease entity appears to be higher than
rest of the world. It also affects younger population; as a result has significant
bearing on the socioeconomic outcome of entire family. There is a strong need
to develop a modified treatment protocol for this disease than conventional
adenocarcinoma as the disease biology appears to be different and standard
chemotherapy doesnt act well on the peritoneal disease. Certain molecular
abnormalities are also noted in SRCC such as high microsatellite instability,
EpCAM mutations, high-frequency of CpG island methylator phenotype, higher
methylation level of long interspersed nucleotide element-1 and frequent BRAF
mutation and low COX-2 expression. Further research needs to be carried out to
understand the biology of this disease entity well which might give us an insight
into potential treatment options for the same.

Applications

To summarize, SRCC seems to be a suitable target for peritoneum directed

therapies which include aggressive cytoreduction and HIPEC. Extended/
modified chemotherapy protocols may improve survival. Further understanding of
molecular biology of this disease may open new methods for its treatment.

Peer-review

It is a retrospective study of an uncommon subtype of colorectal carcinoma. The

author statisized some information of this cancer including the age, location,
stages, metastasis, recurrence and survival.

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E- Editor: Wu HL

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